Microorganisms最新文献_第2页

When Precision Matters: Bone Marrow Cytology Meets qPCR in a Pilot Study Quantifying Leishmania infantum Load in Dogs. 当精度问题：骨髓细胞学与qPCR在一项初步研究定量利什曼原虫在狗的婴儿负荷。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-21 DOI: 10.3390/microorganisms13092211

Juliana Costa, Ana Rita Silva, Filipe Sampaio, Ana Patrícia Alves, Hugo Silva, Hugo Lima de Carvalho, Carlos Sousa, Manuel Simões, Cátia Fernandes, Ana Patrícia Lopes, Andreia Garcês, Elsa Leclerc Duarte, Ana Cláudia Coelho, Luís Cardoso, Ricardo Lopes

{"title":"When Precision Matters: Bone Marrow Cytology Meets qPCR in a Pilot Study Quantifying Leishmania infantum Load in Dogs.","authors":"Juliana Costa, Ana Rita Silva, Filipe Sampaio, Ana Patrícia Alves, Hugo Silva, Hugo Lima de Carvalho, Carlos Sousa, Manuel Simões, Cátia Fernandes, Ana Patrícia Lopes, Andreia Garcês, Elsa Leclerc Duarte, Ana Cláudia Coelho, Luís Cardoso, Ricardo Lopes","doi":"10.3390/microorganisms13092211","DOIUrl":"10.3390/microorganisms13092211","url":null,"abstract":"Leishmania infantum is the causative agent of canine leishmaniosis (CanL), a zoonotic disease of considerable clinical and epidemiological concern. Quantification of parasite load is valuable for clinical management, particularly in low-parasite-load cases. This pilot study evaluated the correlation between cytological and molecular techniques in bone marrow samples from dogs clinically affected with leishmaniosis. Amastigotes were quantified by two independent observers using light microscopy, and the same samples were analysed by real-time polymerase chain reaction (qPCR) employing Leishmania spp. specific commercial primers. Inter-observer agreement was moderate according to Cohen's kappa (κ = 0.47) and substantial according to the intraclass correlation coefficient (ICC = 0.63), respectively, confirming measurement reproducibility. A very strong inverse correlation was found between parasite counts and qPCR cycle threshold (Ct) values (Spearman's ρ = -0.89; p < 0.001). Furthermore, a robust logarithmic relationship (amastigotes/μL = 10(-0.158 × Ct + 7.61); R2 = 0.99994) was established allowing direct estimation of in vivo parasite concentration from molecular data. These preliminary findings suggest that qPCR can serve as a reliable, semi-quantitative tool, offering higher sensitivity in subclinical infections. The integration of molecular quantification with cytological methods enhances diagnostic accuracy and supports personalised therapeutic decision-making, advancing clinical management of CanL in a One Health context.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroAIbiome: Decoding Cancer Types from Microbial Profiles Using Explainable Machine Learning. MicroAIbiome：使用可解释的机器学习从微生物谱中解码癌症类型。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-21 DOI: 10.3390/microorganisms13092210

Md Motiur Rahman, Shiva Shokouhmand, Saeka Rahman, Nafisa Nawar Tamzi, Smriti Bhatt, Miad Faezipour

{"title":"MicroAIbiome: Decoding Cancer Types from Microbial Profiles Using Explainable Machine Learning.","authors":"Md Motiur Rahman, Shiva Shokouhmand, Saeka Rahman, Nafisa Nawar Tamzi, Smriti Bhatt, Miad Faezipour","doi":"10.3390/microorganisms13092210","DOIUrl":"10.3390/microorganisms13092210","url":null,"abstract":"Microbial communities within human tissues are increasingly recognized as promising biomarkers for cancer detection. However, leveraging microbiome data for multiclass cancer classification remains challenging due to its compositional structure, high dimensionality, and lack of model interpretability. In this study, we address these challenges by introducing MicroAIbiome, a machine learning-based artificial intelligence (AI) pipeline designed to classify five cancer types such as esophageal carcinoma (ESCA), head and neck squamous cell carcinoma (HNSC), stomach adenocarcinoma (STAD), colon adenocarcinoma (COAD), and rectum adenocarcinoma (READ), using genus-level microbial relative abundances. Our pipeline incorporates zero-replacement, centered log-ratio (CLR) transformation, correlation filtering, and recursive feature elimination (RFE) to enable robust learning from compositional data. Among five evaluated classifiers, XGBoost achieved the highest accuracy of 78.23%, outperforming prior work. We further enhance interpretability using SHapley Additive exPlanations (SHAP)-based feature attribution to uncover class-specific microbial signatures, such as Corynebacterium in ESCA and Bacteroides in COAD. Our results highlight the importance of compositional preprocessing and explainable AI in advancing microbiome-based cancer diagnostics.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12473104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Challenges of Cyclosporiasis in Chronic Diarrhea: A Case Study. 慢性腹泻中环孢子虫病的诊断挑战：一个案例研究。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-21 DOI: 10.3390/microorganisms13092209

Estera Banasik, Agnieszka Dobrowolska, Lucyna Woźnicka-Leśkiewicz, Piotr Eder

{"title":"Diagnostic Challenges of Cyclosporiasis in Chronic Diarrhea: A Case Study.","authors":"Estera Banasik, Agnieszka Dobrowolska, Lucyna Woźnicka-Leśkiewicz, Piotr Eder","doi":"10.3390/microorganisms13092209","DOIUrl":"10.3390/microorganisms13092209","url":null,"abstract":"Cyclosporiasis, caused by Cyclospora cayetanensis, is a rare opportunistic infection, particularly in immunosuppressed patients with inflammatory bowel disease (IBD). Its clinical presentation may mimic IBD, with chronic diarrhea and anemia resistant to standard therapy. We report the case of a 24-year-old woman with ulcerative colitis (UC) and a history of liver transplantation, treated with vedolizumab and immunosuppressants. Despite endoscopic remission, she experienced persistent abdominal pain, diarrhea, and iron deficiency anemia. Escalation of biologic therapy was ineffective. After exclusion of bacterial and viral causes, stool testing identified Cyclospora cayetanensis. Treatment with nitazoxanide led to rapid clinical and laboratory improvement. Biologic therapy was temporarily discontinued and later resumed without recurrence of symptoms. This case shows that chronic diarrhea in IBD patients may not always result from the underlying disease. Immunosuppression increases the risk of opportunistic infections. Early diagnosis and specific treatment can improve outcomes and allow safe continuation of IBD therapy.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Herpesvirus 6 Activates NF-κB Signalling and CD163-Positive Macrophage Recruitment in Alcohol-Induced Hepatic Injury. 人疱疹病毒6在酒精性肝损伤中激活NF-κB信号和cd163阳性巨噬细胞募集

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-20 DOI: 10.3390/microorganisms13092204

Anda Upane, Simons Svirskis, Valerija Groma, Sandra Skuja

{"title":"Human Herpesvirus 6 Activates NF-κB Signalling and CD163-Positive Macrophage Recruitment in Alcohol-Induced Hepatic Injury.","authors":"Anda Upane, Simons Svirskis, Valerija Groma, Sandra Skuja","doi":"10.3390/microorganisms13092204","DOIUrl":"10.3390/microorganisms13092204","url":null,"abstract":"Human herpesvirus 6 (HHV-6) establishes lifelong latency in immune cells and may contribute to the progression of ethanol-induced liver injury. To elucidate the contribution of HHV-6 to alcohol-induced hepatic injury, this study evaluated HHV-6 protein expression, NF-κB signalling, and CD163-positive macrophage recruitment in liver samples from control subjects, young individuals with recent alcohol exposure, and individuals with long-term chronic alcohol use. Liver lobules displaying HHV-6 positivity were more frequent in alcohol users (64% in young and 72% in chronic users) compared to controls (48%). CD163-positive macrophage counts were higher in both young and chronic alcohol users compared to controls, with the greatest increase in HHV-6-positive chronic users. NF-κB expression intensity was elevated in alcohol users (p < 0.005), and further increased in HHV-6-positive samples (p = 0.02). These findings indicate an association between HHV-6 persistence, NF-κB pathway activation, and CD163-positive macrophage-driven inflammatory responses in liver tissue under conditions of chronic alcohol use. Further research is warranted to uncover the mechanisms underlying the interaction between HHV-6 and ethanol in liver injury.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postbiotic Effects of Pediococcus acidophilus LS for Anti-Melanogenesis, Photoprotection, and Wound Repair. 嗜酸Pediococcus ophilus LS在抗黑素生成、光保护和伤口修复中的作用。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-20 DOI: 10.3390/microorganisms13092207

Chiung-Hung Chang, Jai-Sing Yang, Yen-Ju Lai, Bi Yu, Yuan-Man Hsu

{"title":"Postbiotic Effects of Pediococcus acidophilus LS for Anti-Melanogenesis, Photoprotection, and Wound Repair.","authors":"Chiung-Hung Chang, Jai-Sing Yang, Yen-Ju Lai, Bi Yu, Yuan-Man Hsu","doi":"10.3390/microorganisms13092207","DOIUrl":"10.3390/microorganisms13092207","url":null,"abstract":"Skin health is significantly impacted by factors such as melanin production, UV-induced photodamage, and wound healing. Excessive melanin leads to hyperpigmentation, while UVA radiation accelerates skin aging and oxidative stress. This study investigated the multi-functional dermatological potential of S strain LS-derived cell-free supernatant (CFS-LS) to address these concerns. Our findings demonstrate that CFS-LS effectively inhibits melanogenesis in B16F10 cells. It significantly reduced α-MSH-induced melanin synthesis, comparable to arbutin, by downregulating key melanogenic enzymes (tyrosinase, TRP-1, and TRP-2) and regulatory proteins (p-CREB, MITF, SOX9, and SOX10). Mechanistically, CFS-LS suppressed the phosphorylation of MEK, ERK, p38, and JNK, indicating a dual inhibitory effect on both PKA/CREB and MAPK pathways. Furthermore, CFS-LS mitigated UVA-induced photodamage in HaCaT cells by significantly reducing intracellular reactive oxygen species and suppressing the downstream phosphorylation of p53 and α-MSH levels. It also restored UVA-suppressed Nrf-2 and HO-1 expression, enhancing cellular antioxidant defenses. Lastly, CFS-LS promoted skin wound healing by significantly enhancing HaCaT cell migration in a scratch assay, associated with increased p-MEK1/2 and p-ERK1/2 levels, and notably elevated collagen type I synthesis. Collectively, these results highlight CFS-LS as a potent multi-functional agent for skin protection and repair, with significant potential for cosmetic and therapeutic applications. The active components of CFS-LS warrant further investigation.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12473142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Potential of Contamination Remediation and Carbon Fixation: Functional Resilience of Carbon Fixation in Petroleum Hydrocarbon-Degrading Microbial Communities Under Enhanced Natural Attenuation. 污染修复和固碳的协同潜力：自然衰减增强下石油烃降解微生物群落固碳的功能弹性。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-20 DOI: 10.3390/microorganisms13092205

Pingping Cai, Shuang Gan, Zhuo Ning, Min Zhang

{"title":"Synergistic Potential of Contamination Remediation and Carbon Fixation: Functional Resilience of Carbon Fixation in Petroleum Hydrocarbon-Degrading Microbial Communities Under Enhanced Natural Attenuation.","authors":"Pingping Cai, Shuang Gan, Zhuo Ning, Min Zhang","doi":"10.3390/microorganisms13092205","DOIUrl":"10.3390/microorganisms13092205","url":null,"abstract":"Enhanced Natural Attenuation (ENA) can accelerate pollutant degradation by adding electron acceptors or nutrients. However, its impact on carbon-fixing microorganisms, which are widely found in the natural attenuation process, remains unclear. In this study, four types of ENA materials were added in batch experiments. Chemical analysis and metagenomic sequencing were employed to analyze the degradation kinetics of petroleum hydrocarbons, the consumption pattern of nitrate, as well as the functional genes and population evolution characteristics of carbon-fixing microorganisms. Results showed that nitrate-based enhancement materials significantly improved the petroleum hydrocarbon degradation rate but suppressed the expression of some carbon fixation genes, such as those involved in the Calvin-Benson-Bassham cycle. Nevertheless, the overall abundance of carbon fixation genes did not show a notable decline. Dominant bacterial genera such as Pseudomonas and Achromobacter possessed both hydrocarbon degradation and carbon fixation capabilities. Although the calcium peroxide treatment group only achieved a 40% petroleum hydrocarbon degradation rate, it significantly promoted the abundance of carbon fixation genes involved in the reductive tricarboxylic acid cycle pathway. Therefore, ENA alters carbon fixation pathways but does not diminish carbon fixation potential, indicating its potential for synergistically achieving pollution remediation and carbon fixation.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Transcriptomics Reveals Distinct Adaptation Mechanisms for Degradation of n-Alkane and Branched Alkane in the Salt-Tolerant Bacterium Dietzia sp. CN-3. 比较转录组学揭示耐盐细菌Dietzia sp. CN-3降解正构烷烃和支构烷烃的不同适应机制。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-20 DOI: 10.3390/microorganisms13092206

Weiwei Chen, Jiawei Sun, Xin Zhang, Jiawen Zhang, Yuan Wang, Shiwei Cheng

{"title":"Comparative Transcriptomics Reveals Distinct Adaptation Mechanisms for Degradation of n-Alkane and Branched Alkane in the Salt-Tolerant Bacterium Dietzia sp. CN-3.","authors":"Weiwei Chen, Jiawei Sun, Xin Zhang, Jiawen Zhang, Yuan Wang, Shiwei Cheng","doi":"10.3390/microorganisms13092206","DOIUrl":"10.3390/microorganisms13092206","url":null,"abstract":"Although hydrocarbon-degrading bacteria utilize a wide range of alkanes, the global metabolic features and regulatory mechanisms governing their growth on alkanes with different chain lengths remain incompletely elucidated. In this study, we analyzed the comparative transcriptomics of a salt-tolerant bacterium, Dietzia sp. CN-3, to investigate molecular adaptations and metabolic processes when grown on n-hexadecane (C16), branched alkane (pristane), and glucose. A total of 1766 differentially expressed genes (DEGs) were identified in the C16 group compared to the glucose control, with 1024 (58.0%) being upregulated and 742 (42.0%) being downregulated. Notably, the pristane group exhibited 1542 DEGs, of which 488 (31.6%) were upregulated and 1054 (68.4%) were downregulated. Our results demonstrate that C16 and pristane induced common genes of alkane hydroxylation in the core alkane degradation pathway, while eliciting distinct transcriptional patterns of genes involved in lipid metabolism, energy metabolism, metal ion transportation, cell surface composition biosynthesis, and transcription regulation. The findings reveal that CN-3 employs diverse metabolic strategies to adapt to alkanes with different chain lengths, displaying considerable metabolic plasticity. This study significantly enhances our understanding of molecular adaptation of bacteria to hydrocarbon-containing environments and may provide valuable information for further studies of petroleum hydrocarbon bioremediation.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periorbital and Central Nervous System Infection Due to Arcanobacterium haemolyticum: Case Report and Review of the Literature. 溶血隐菌引起的眶周及中枢神经系统感染：病例报告及文献复习。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-20 DOI: 10.3390/microorganisms13092208

Pierangelo Chinello, Alessandro Capone, Samir Al Moghazi, Paolo Cirillo, Carla Fontana, Stefania Cicalini

{"title":"Periorbital and Central Nervous System Infection Due to Arcanobacterium haemolyticum: Case Report and Review of the Literature.","authors":"Pierangelo Chinello, Alessandro Capone, Samir Al Moghazi, Paolo Cirillo, Carla Fontana, Stefania Cicalini","doi":"10.3390/microorganisms13092208","DOIUrl":"10.3390/microorganisms13092208","url":null,"abstract":"Arcanobacterium haemolyticum is a facultative anaerobe, catalase-negative, Gram-positive pleomorphic rod, most commonly responsible for pharyngeal infections. Invasive A. haemolyticum infections are rare and typically involve immunocompromised patients; however, severe and invasive infections in immunocompetent patients have also been reported. Here we describe a case of sinusitis complicated by periorbital cellulitis, subdural empyema and cerebritis due to A. haemolyticum in an immunocompetent patient. The patient was initially treated with daptomycin + piperacillin/tazobactam and subsequently with linezolid + meropenem and required multiple surgical interventions to attain source control. Although uncommon, A. haemolyticum should be considered as a causative agent of severe infections complicating pharyngitis or sinusitis that may result from local extension or haematogenous spread, even in an immunocompetent host. We also present a literature review on central nervous system involvement by A. haemolyticum infection.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an RPA-CRISPR/LbaCas12a-Lateral Flow Assay for the Visual Detection of Chrysotila dentata (Haptophyta). RPA-CRISPR/ lbacas12a -侧流法视觉检测齿状黄霉的建立

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-20 DOI: 10.3390/microorganisms13092203

Jiating Yu, Yun Shen, Qinfei Zhang, Xuxu Luo, Yujie Zong, Chengxu Zhou, Hailong Huang, Haibo Jiang

{"title":"Development of an RPA-CRISPR/LbaCas12a-Lateral Flow Assay for the Visual Detection of Chrysotila dentata (Haptophyta).","authors":"Jiating Yu, Yun Shen, Qinfei Zhang, Xuxu Luo, Yujie Zong, Chengxu Zhou, Hailong Huang, Haibo Jiang","doi":"10.3390/microorganisms13092203","DOIUrl":"10.3390/microorganisms13092203","url":null,"abstract":"Chrysotila dentata (Haptophyta), a harmful algal bloom (HAB) species frequently occurring in coastal waters of China, is one with strong environmental adaptability that poses a serious threat to marine ecosystems and fisheries. Current molecular detection techniques and early warning systems for this species remain limited. To address this, we developed a rapid and highly sensitive detection method for C. dentata. This method integrates recombinase polymerase amplification (RPA) with CRISPR-LbaCas12a and lateral flow dipstick (LFD) technologies, enabling visual readout of results. Key parameters, including the single-stranded DNA (ssDNA) reporter concentration, reaction time, and temperature, were systematically optimized. Field water sample testing demonstrated high specificity and sensitivity, achieving a detection limit of 5 × 10-6 pg μL-1 for genomic DNA under laboratory conditions and 2.82 × 101 cells mL-1 in simulated environmental samples. The entire detection process takes only 1 h (at a constant 39 °C), and results can be directly interpreted via LFD strips. For early warning and prevention of C. dentata outbreaks, this assay provides a powerful, reliable, and field-ready monitoring tool.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12473113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Research of the Stress Response of Saccharomyces cerevisiae W303 Yeast to Metal Ions Eluted from Orthodontic Appliances. 酿酒酵母菌W303对正畸器中金属离子胁迫反应的蛋白质组学研究。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-19 DOI: 10.3390/microorganisms13092200

Lara Dežulović, Božena Ćurko-Cofek, Gordana Čanadi Jurešić

{"title":"Proteomic Research of the Stress Response of Saccharomyces cerevisiae W303 Yeast to Metal Ions Eluted from Orthodontic Appliances.","authors":"Lara Dežulović, Božena Ćurko-Cofek, Gordana Čanadi Jurešić","doi":"10.3390/microorganisms13092200","DOIUrl":"10.3390/microorganisms13092200","url":null,"abstract":"Although orthodontic appliances are widely used in daily practice, they also have their downsides due to the large amount of metal ions released from their surface. In this study, the influence of such released metal ions on the yeast Saccharomyces cerevisiae W303 as a model organism was investigated. Experimental yeast media in which metal ions (iron, aluminum, nickel, chromium, copper, and manganese) were eluted for 3, 7, 14, and 28 days were prepared and then used for yeast cultivation (up to the early stationary growth phase). The growth, increase, and viability of the cells were tested. The mitochondria were isolated from the spheroplasts, and the mitochondrial proteins were obtained and analyzed by liquid chromatography/mass spectrometry. Fortythree significantly altered proteins were identified. They showed significantly reduced expression in all metal-treated groups compared to the control. The metabolic processes for energy supply (glycolysis, gluconeogenesis, tricarboxylic acid cycle, and adenosine triphosphate synthesis) dominated with 50% of the total amount of significantly altered proteins in all samples, but their proportions changed at different time points. The downregulation of mitochondrial proteins such as Atp1, Atp2, and Pet9 under conditions of metal overload suggests a broader impairment of mitochondrial function. Three levels of response to stress can be observed-at relatively low metal ion concentrations in the medium (3 days of elution, approx. 3 mg/L), at medium concentrations (7 days of elution, approx. 5.5 mg/L), and at high concentrations (>8 mg/L, 14 and 28 days of elution), each affecting a specific group of proteins. The results show that mixtures of metal ions in experimental media led to a specific response (in terms of the amount and type of proteins) in each sample type to combat the provoked stress.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0