Microorganisms最新文献_第2页

PH Nonlinearly Dominates Soil Bacterial Community Assembly along an Altitudinal Gradient in Oak-Dominant Forests PH 非线性主导橡树林海拔梯度上的土壤细菌群落组合

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-11 DOI: 10.3390/microorganisms12091877

Litao Lin, Guixiang Li, Huiyi Yu, Keming Ma

{"title":"PH Nonlinearly Dominates Soil Bacterial Community Assembly along an Altitudinal Gradient in Oak-Dominant Forests","authors":"Litao Lin, Guixiang Li, Huiyi Yu, Keming Ma","doi":"10.3390/microorganisms12091877","DOIUrl":"https://doi.org/10.3390/microorganisms12091877","url":null,"abstract":"Soil bacteria, the predominant microbiota in soil, are subject to the law of minimum and the law of tolerance, but the assembly patterns of soil bacteria in response to environmental factors remain far from clear. Here, we took advantage of an altitudinal gradient (1020–1770 asl) in oak-dominant forests and assessed whether soil bacteria linearly or nonlinearly respond to environmental properties through the changes in the community diversity and composition. We found that soil bacteria decreased with increasing altitude in terms of the species richness and phylogenetic structure, while they were unchanged with increasing altitude in terms of community composition. The species richness was nonlinearly affected by the soil pH (19.9%), C:N ratio (14.3%), SOC (11.4%), and silt+clay content (9.9%). Specifically, the species richness peaked at a pH of 5.5–6.5, and an SOC of 25–50 g kg−1, and it showed abrupt decreases and increases at a C:N ratio of 14.5 and a silt+clay content of 70%. The community composition was significantly affected by the soil pH (28.2%), then by the SOC (3.6%), available phosphorus (1.0%), and silt+clay content (0.5%), and it showed less turnovers at a pH of 6.0, SOC of 50 g kg−1, and available phosphorus > 3.0 g kg−1. These findings imply that environmental filtering processes nonlinearly shape bacterial communities.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China 中国长距离引水工程蓄水池真核微生物群落的季节性变化

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-11 DOI: 10.3390/microorganisms12091873

Yingying Yang, Fangfang Ci, Ailing Xu, Xijian Zhang, Ning Ding, Nianxin Wan, Yuanyuan Lv, Zhiwen Song

{"title":"Seasonal Dynamics of Eukaryotic Microbial Communities in the Water-Receiving Reservoir of the Long-Distance Water Diversion Project, China","authors":"Yingying Yang, Fangfang Ci, Ailing Xu, Xijian Zhang, Ning Ding, Nianxin Wan, Yuanyuan Lv, Zhiwen Song","doi":"10.3390/microorganisms12091873","DOIUrl":"https://doi.org/10.3390/microorganisms12091873","url":null,"abstract":"Inter-basin water transfer projects, such as the Yellow River to Qingdao Water Diversion Project (YQWD), are essential for addressing water scarcity, but impact local aquatic ecosystems. This study investigates the seasonal characteristics of eukaryotic microbial communities in the Jihongtan Reservoir, the main water-receiving body of YQWD, over a one-year period using 18S rDNA amplicon sequencing. The results showed that the eukaryotic microbial diversity did not exhibit significant seasonal variation (p > 0.05), but there was a notable variance in the community structure (p < 0.05). Arthropoda and Paracyclopina, representing the most dominant phylum and the most dominant genus, respectively, both exhibited the lowest abundance during the winter. The Chlorophyta, as the second-dominant phylum, demonstrates its higher abundance in the spring and winter. The Mantel test and PLS-PM (Partial Least Squares Path Modeling) revealed that water temperature (WT), dissolved oxygen (DO), and pH influenced the seasonal dynamic of eukaryotic microbial communities significantly, of which WT was the primary driving factor. In addition to environmental factors, water diversion is likely to be an important influencing factor. The results of the co-occurrence network and robustness suggested that the spring network is the most complex and exhibits the highest stability. Moreover, keystone taxa within networks have been identified, revealing that these key groups encompass both abundant and rare species, with specificity to different seasons. These insights are vital for understanding the seasonal variation of microbial communities in the Jihongtan Reservoir during ongoing water diversions.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance Comparison of Four Hepatitis E Antibodies Detection Methods 四种戊型肝炎抗体检测方法的性能比较

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-11 DOI: 10.3390/microorganisms12091875

Milagros Muñoz-Chimeno, Nazaret Díaz-Sánchez, Lucía Morago, Vanessa Rodríguez-Paredes, Silvia Barturen, Álvaro Rodríguez-Recio, Maira Alejandra García-Lugo, Maria Isabel Zamora, María Mateo, Mónica Sánchez-Martínez, Ana Avellón

{"title":"Performance Comparison of Four Hepatitis E Antibodies Detection Methods","authors":"Milagros Muñoz-Chimeno, Nazaret Díaz-Sánchez, Lucía Morago, Vanessa Rodríguez-Paredes, Silvia Barturen, Álvaro Rodríguez-Recio, Maira Alejandra García-Lugo, Maria Isabel Zamora, María Mateo, Mónica Sánchez-Martínez, Ana Avellón","doi":"10.3390/microorganisms12091875","DOIUrl":"https://doi.org/10.3390/microorganisms12091875","url":null,"abstract":"HEV antibody detection constitutes the main screening test for HEV infection. The aim of this study is to compare the sensitivity and specificity of four techniques: LIAISON® MUREX DiaSorin anti-HEV IgG and anti-HEV IgM assays, Hepatitis E VIRCLIA® IgM and IgG monotests, WANTAI HEV-IgM and IgG ELISA and VIDAS® anti-HEV IgM and IgG tests in five panels of samples configurated according to the immunoblot (RecomLine, Mikrogen, Neuss, Germany). Anti-HEV IgM sensitivity in the acute phase was 100% in all techniques, while sensitivity, including the immediate convalescence phase, was 96.74% for LIAISON®, 83.14% for VIRCLIA®, 84.78% for WANTAI and 88.04% for VIDAS®. Anti-HEV IgM specificity was 100% for both LIAISON® and VIRCLIA®. Anti-HEV IgM WANTAI agreed with VIRCLIA® with a good Kappa coefficient (κ = 0.71). Anti-HEV IgG post-infection sensitivity was 100% for LIAISON®, VIDAS® and VIRCLIA® and 99% for WANTAI. Anti-HEV IgG specificity reached 97.17% for LIAISON and 88.68% for VIRCLIA®. Our results demonstrated a better capacity of LIAISON® MUREX anti-HEV IgM than that of competitors for detecting acute infections as well as accurate anti-HEV IgG results and in how to resolve them.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update of the Genetic Variability of Monkeypox Virus Clade IIb Lineage B.1 猴痘病毒 IIb 支系 B.1 遗传变异的最新情况

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-11 DOI: 10.3390/microorganisms12091874

Fabio Scarpa, Ilenia Azzena, Alessandra Ciccozzi, Francesco Branda, Chiara Locci, Maria Perra, Noemi Pascale, Chiara Romano, Giancarlo Ceccarelli, Giuseppe Terrazzano, Pier Luigi Fiori, Massimo Ciccozzi, Marco Casu, Daria Sanna

{"title":"Update of the Genetic Variability of Monkeypox Virus Clade IIb Lineage B.1","authors":"Fabio Scarpa, Ilenia Azzena, Alessandra Ciccozzi, Francesco Branda, Chiara Locci, Maria Perra, Noemi Pascale, Chiara Romano, Giancarlo Ceccarelli, Giuseppe Terrazzano, Pier Luigi Fiori, Massimo Ciccozzi, Marco Casu, Daria Sanna","doi":"10.3390/microorganisms12091874","DOIUrl":"https://doi.org/10.3390/microorganisms12091874","url":null,"abstract":"From 1 January 2022 to 31 May 2024, the World Health Organization (WHO) reported 97,745 laboratory-confirmed Mpox cases, including 203 deaths, across 116 countries. Despite a 2.3% decrease in new cases in May 2024 compared to April 2024, significant regional variations persist. The African Region reported the highest proportion of new cases, while other regions experienced mixed trends. Phylogenomic analyses of the Mpox virus Clade IIb lineage B.1 reveal stable genetic variability with minimal diversification. The Bayesian Skyline Plot indicates a generally stable viral population size with a modest peak in late 2023, followed by a decline. In general, the data indicate that the MPXV outbreak is primarily localized within a few consistent geographic clusters. The virus’s evolution is relatively slow, as indicated by its stable genetic variability, and Clade IIb lineage B.1 does not currently show signs of rapid genetic changes or population growth. The current low level of genetic diversity should not lead to complacency. Ongoing genomic surveillance is essential for effective outbreak management and understanding. This monitoring is crucial for identifying any shifts in the virus’s behavior or transmission, allowing for prompt public health responses and adjustments. In addition, continued vigilance is necessary to detect any new variants that might influence the outbreak’s trajectory.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypic Stability of Lactic Acid Bacteria in Industrial Rye Bread Sourdoughs Assessed by ITS-PCR Analysis 通过 ITS-PCR 分析评估工业化黑麦面包酸面团中乳酸菌基因型的稳定性

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-11 DOI: 10.3390/microorganisms12091872

Liis Lutter, Aljona Kuzina, Helena Andreson

{"title":"Genotypic Stability of Lactic Acid Bacteria in Industrial Rye Bread Sourdoughs Assessed by ITS-PCR Analysis","authors":"Liis Lutter, Aljona Kuzina, Helena Andreson","doi":"10.3390/microorganisms12091872","DOIUrl":"https://doi.org/10.3390/microorganisms12091872","url":null,"abstract":"Sourdough bread production relies on metabolically active starters refreshed daily with flour and water. The stability of sourdough microbial strains is crucial for consistent bread quality. However, many bakeries lack information on the persistence of starter cultures in ongoing sourdough production. Consequently, there is growing interest in identifying microbial strains from regularly used sourdoughs that possess good functional properties and resist changes in the complex growth environment. This study aimed to evaluate the composition and stability of lactic acid bacteria (LAB) in industrial wheat (WS) and rye (RS) sourdoughs propagated over a long period. LAB isolates (n = 66) from both sourdoughs, sampled over four seasons, were identified using phenotypic methods and genotyped via ITS-PCR and ITS-PCR/TaqI restriction analysis. Eight LAB species were detected, with Lactiplantibacillus plantarum being the most dominant and stable. Nineteen distinct LAB genotypes were observed, highlighting significant diversity. The presence of identical LAB genotypes in both sourdoughs suggests microbial transfer through the environment and bakery workers. LAB in RS were found to be more stable than those in WS. These findings underscore the importance of monitoring microbial stability and diversity in industrial sourdough production to maintain consistent bread quality.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Utilization of Prebiotics by Listeria monocytogenes 李斯特菌对益生元的体外利用

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-11 DOI: 10.3390/microorganisms12091876

Tereza Kodešová, Anna Mašlejová, Eva Vlková, Šárka Musilová, Kristýna Horváthová, Hana Šubrtová Salmonová

{"title":"In Vitro Utilization of Prebiotics by Listeria monocytogenes","authors":"Tereza Kodešová, Anna Mašlejová, Eva Vlková, Šárka Musilová, Kristýna Horváthová, Hana Šubrtová Salmonová","doi":"10.3390/microorganisms12091876","DOIUrl":"https://doi.org/10.3390/microorganisms12091876","url":null,"abstract":"Listeria monocytognes is an emerging pathogen responsible for the serious foodborne disease, listeriosis. The commensal gut microbiota is the first line of defense against pathogen internalization. The gut microbiome can be modified by prebiotic substrates, which are frequently added to food products and dietary supplements. Prebiotics should selectively support the growth of beneficial microbes and thus improve host health. Nevertheless, little is known about their effect on the growth of L. monocytogenes. The aim of this study was to evaluate the growth ability of four L. monocytogenes strains, representing the most common serotypes, on prebiotic oligosaccharides (beta-(1,3)-D-glucan, inulin, fructooligosaccharides, galactooligosaccharides, lactulose, raffinose, stachyose and 2′-fucosyllactose and a mixture of human milk oligosaccharides) as a sole carbon source. The results showed that only beta-(1,3)-D-glucan was metabolized by L. monocytogenes. These cell culture data suggest that beta-(1,3)-D-glucan may not be selectively utilized by healthy commensal bacteria, and its role in intestinal pathogen growth warrants further exploration in vivo.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Resistance of Staphylococcus spp. from Human Specimens Submitted to Diagnostic Laboratories in South Africa, 2012–2017 2012-2017 年南非诊断实验室采集的人类标本中葡萄球菌的抗菌药耐药性

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-09 DOI: 10.3390/microorganisms12091862

Themba Titus Sigudu, James Wabwire Oguttu, Daniel Nenene Qekwana

{"title":"Antimicrobial Resistance of Staphylococcus spp. from Human Specimens Submitted to Diagnostic Laboratories in South Africa, 2012–2017","authors":"Themba Titus Sigudu, James Wabwire Oguttu, Daniel Nenene Qekwana","doi":"10.3390/microorganisms12091862","DOIUrl":"https://doi.org/10.3390/microorganisms12091862","url":null,"abstract":"Antimicrobial resistance (AMR) poses a significant worldwide health challenge associated with prolonged illnesses, increased healthcare costs, and high mortality rates. The present study examined the patterns and predictors of AMR among human Staphylococcus isolates obtained from diagnostic laboratories in South Africa between 2012 and 2017. This study examined data from 404 217 isolates, assessing resistance rates across different characteristics such as age, sample origin, Staphylococcus species, and study period. The highest resistance was observed against cloxacillin (70.3%), while the lowest resistance was against Colistin (0.1%). A significant (p < 0.05) decreasing trend in AMR was observed over the study period, while a significant increasing temporal trend (p < 0.05) was observed for multidrug resistance (MDR) over the same period. A significant (p < 0.05) association was observed between specimen type, species of organism, and year of isolation with AMR outcome. Significant (p < 0.05) associations were observed between specimen type and season with MDR. The observed high levels of AMR and a growing trend in MDR are concerning for public health. Clinicians should take these findings into account when deciding on therapeutic options. Continued monitoring of AMR among Staphylococcus spp. and judicious use of antimicrobials in human medicine should be promoted.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Slope-Matrix-Graph Algorithm to Analyze Compositional Microbiome Data 分析微生物组合成数据的新型斜坡矩阵图算法

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-09 DOI: 10.3390/microorganisms12091866

Meng Zhang, Xiang Li, Adelumola Oladeinde, Michael Rothrock, Anthony Pokoo-Aikins, Gregory Zock

{"title":"A Novel Slope-Matrix-Graph Algorithm to Analyze Compositional Microbiome Data","authors":"Meng Zhang, Xiang Li, Adelumola Oladeinde, Michael Rothrock, Anthony Pokoo-Aikins, Gregory Zock","doi":"10.3390/microorganisms12091866","DOIUrl":"https://doi.org/10.3390/microorganisms12091866","url":null,"abstract":"Networks are widely used to represent relationships between objects, including microorganisms within ecosystems, based on high-throughput sequencing data. However, challenges arise with appropriate statistical algorithms, handling of rare taxa, excess zeros in compositional data, and interpretation. This work introduces a novel Slope-Matrix-Graph (SMG) algorithm to identify microbiome correlations primarily based on slope-based distance calculations. SMG effectively handles any proportion of zeros in compositional data and involves: (1) searching for correlated relationships (e.g., positive and negative directions of changes) based on a “target of interest” within a setting, and (2) quantifying graph changes via slope-based distances between objects. Evaluations on simulated datasets demonstrated SMG’s ability to accurately cluster microbes into distinct positive/negative correlation groups, outperforming methods like Bray–Curtis and SparCC in both sensitivity and specificity. Moreover, SMG demonstrated superior accuracy in detecting differential abundance (DA) compared to ZicoSeq and ANCOM-BC2, making it a robust tool for microbiome analysis. A key advantage is SMG’s natural capacity to analyze zero-inflated compositional data without transformations. Overall, this simple yet powerful algorithm holds promise for diverse microbiome analysis applications.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 Genotyping Highlights the Challenges in Spike Protein Drift Independent of Other Essential Proteins SARS-CoV-2 基因分型凸显了尖峰蛋白漂移与其他重要蛋白无关的难题

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-09 DOI: 10.3390/microorganisms12091863

Jeremy W. Prokop, Sheryl Alberta, Martin Witteveen-Lane, Samantha Pell, Hosam A. Farag, Disha Bhargava, Robert M. Vaughan, Austin Frisch, Jacob Bauss, Humza Bhatti, Sanjana Arora, Charitha Subrahmanya, David Pearson, Austin Goodyke, Mason Westgate, Taylor W. Cook, Jackson T. Mitchell, Jacob Zieba, Matthew D. Sims, Adam Underwood, Habiba Hassouna, Surender Rajasekaran, Maximiliano A. Tamae Kakazu, Dave Chesla, Rosemary Olivero, Adam J. Caulfield

{"title":"SARS-CoV-2 Genotyping Highlights the Challenges in Spike Protein Drift Independent of Other Essential Proteins","authors":"Jeremy W. Prokop, Sheryl Alberta, Martin Witteveen-Lane, Samantha Pell, Hosam A. Farag, Disha Bhargava, Robert M. Vaughan, Austin Frisch, Jacob Bauss, Humza Bhatti, Sanjana Arora, Charitha Subrahmanya, David Pearson, Austin Goodyke, Mason Westgate, Taylor W. Cook, Jackson T. Mitchell, Jacob Zieba, Matthew D. Sims, Adam Underwood, Habiba Hassouna, Surender Rajasekaran, Maximiliano A. Tamae Kakazu, Dave Chesla, Rosemary Olivero, Adam J. Caulfield","doi":"10.3390/microorganisms12091863","DOIUrl":"https://doi.org/10.3390/microorganisms12091863","url":null,"abstract":"As of 2024, SARS-CoV-2 continues to propagate and drift as an endemic virus, impacting healthcare for years. The largest sequencing initiative for any species was initiated to combat the virus, tracking changes over time at a full virus base-pair resolution. The SARS-CoV-2 sequencing represents a unique opportunity to understand selective pressures and viral evolution but requires cross-disciplinary approaches from epidemiology to functional protein biology. Within this work, we integrate a two-year genotyping window with structural biology to explore the selective pressures of SARS-CoV-2 on protein insights. Although genotype and the Spike (Surface Glycoprotein) protein continue to drift, most SARS-CoV-2 proteins have had few amino acid alterations. Within Spike, the high drift rate of amino acids involved in antibody evasion also corresponds to changes within the ACE2 binding pocket that have undergone multiple changes that maintain functional binding. The genotyping suggests selective pressure for receptor specificity that could also confer changes in viral risk. Mapping of amino acid changes to the structures of the SARS-CoV-2 co-transcriptional complex (nsp7-nsp14), nsp3 (papain-like protease), and nsp5 (cysteine protease) proteins suggest they remain critical factors for drug development that will be sustainable, unlike those strategies targeting Spike.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactic Acid Bacteria–Gut-Microbiota-Mediated Intervention towards Inflammatory Bowel Disease 乳酸菌-肠道微生物群介导的炎症性肠病干预措施

IF 4.5 2区生物学

Microorganisms Pub Date : 2024-09-09 DOI: 10.3390/microorganisms12091864

Diantong Li, Zhenjiang Liu, Xueni Fan, Tingting Zhao, Dongxu Wen, Xiaodan Huang, Bin Li

{"title":"Lactic Acid Bacteria–Gut-Microbiota-Mediated Intervention towards Inflammatory Bowel Disease","authors":"Diantong Li, Zhenjiang Liu, Xueni Fan, Tingting Zhao, Dongxu Wen, Xiaodan Huang, Bin Li","doi":"10.3390/microorganisms12091864","DOIUrl":"https://doi.org/10.3390/microorganisms12091864","url":null,"abstract":"Inflammatory bowel disease (IBD), encompassing ulcerative colitis (UC) and Crohn’s disease (CD), arises from intricate interactions involving genetics, environment, and pharmaceuticals with an ambiguous pathogenic mechanism. Recently, there has been an increasing utilization of lactic acid bacteria (LAB) in managing IBD, attributed to their ability to enhance intestinal barrier function, mitigate inflammatory responses, and modulate gut microbiota. This review initiates by elucidating the pathogenesis of IBD and its determinants, followed by an exploration of the mechanisms underlying LAB therapy in UC and CD. Special attention is directed towards their influence on intestinal barrier function and homeostasis regulated by gut microbiota. Furthermore, the review investigates the complex interplay among pivotal gut microbiota, metabolites, and pathways associated with inflammation. Moreover, it underscores the limitations of LAB in treating IBD, particularly in light of their varying roles in UC and CD. This comprehensive analysis endeavors to offer insights for the optimized application of LAB in IBD therapy.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0