Microorganisms最新文献

{"title":"Current Approaches on Nurse-Performed Interventions to Prevent Healthcare-Acquired Infections: An Umbrella Review.","authors":"Joana Teixeira, Neuza Reis, Ewelina Chawłowska, Paula Rocha, Barbara Czech-Szczapa, Ana Catarina Godinho, Grażyna Bączyk, João Agrelos, Krystyna Jaracz, Carlos Fontoura, Pedro Lucas, M Rosário Pinto","doi":"10.3390/microorganisms13020463","DOIUrl":"10.3390/microorganisms13020463","url":null,"abstract":"<p><p>To analyze nursing interventions for preventing healthcare-associated infections (HAIs), major complications in acute care impacting length of stay, costs, morbidity, and mortality, an umbrella review was conducted between 1 February and 26 February 2024, following the Joanna Briggs Institute methodology and PRISMA reporting guidelines, resulting in the inclusion of 22 articles. The 22 final articles obtained addressed the following Centers for Disease Control and Prevention (CDC) categories: surgical site infections (e.g., skin antisepsis, antibiotic prophylaxis), catheter-related bloodstream infections (e.g., taurolidine lock solutions), ventilator-associated pneumonia (e.g., oral hygiene, semi-recumbent positioning), and catheter-associated urinary tract infections (e.g., catheter duration management). Using Neuman's holistic framework, the review emphasized patient-environment interactions. Further primary research is needed to refine these interventions and enhance interprofessional care. The protocol was registered in PROSPERO (CRD42024506801).</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging Microorganisms to Combat Skin Cancer.","authors":"Hayden J Oyler, Austen W Callister, Makenzi N Kutch, Mark R Wakefield, Yujiang Fang","doi":"10.3390/microorganisms13020462","DOIUrl":"10.3390/microorganisms13020462","url":null,"abstract":"<p><p>Skin cancer, including melanoma and non-melanoma types, presents a significant and growing global health challenge due to its increasing incidence and mortality rates. While conventional treatments such as surgical excision, immunotherapy, and targeted therapies are well-established, microorganism-based approaches represent an innovative and promising alternative. These therapies employ live, genetically engineered, or commensal bacteria, viral vectors, or bacterial components to achieve various therapeutic mechanisms, including tumor targeting, immune system modulation, vascular disruption, competitive exclusion, drug delivery, and direct oncolysis. Despite their potential, these approaches require further investigation to address safety concerns, optimize treatment protocols, and gain a comprehensive understanding of their long-term outcomes.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous Bio-Recycling: Recovering Bioactive Molecules Through Endogenous Microbial Maceration of Hemp Residues.","authors":"Leonardo Donati, Angela Conti, Debora Casagrande Pierantoni, Chiara Ruspi, Martina Cerri, Carla Emiliani, Gianluigi Cardinali, Laura Corte","doi":"10.3390/microorganisms13020455","DOIUrl":"10.3390/microorganisms13020455","url":null,"abstract":"<p><p>Biomass residues represent a major issue for industries. On the other hand, residues enclosed major classes of bioactive compounds that could be extracted and used across various fields. This study aimed to elucidate the role of the endogenous microbial community in the lignocellulosic degradation of hemp residues for biorefineries or other industrial processes, simultaneously characterizing the composition of three extracts recovered at different stages of maceration. The process was examined from different perspectives: plant tissue degradation and microbial dynamics were monitored using histological, cultural-dependent, and independent analysis. Extracts were characterized through FTIR, NMR, and SDS-PAGE analyses, also evaluating their potential as biostimulants for microbial growth. Results revealed that the composition of the endogenous prokaryotic community remained stable during the maceration period, while fluctuations in various fungal genera were observed. The taxonomical composition of hemp residues at different stages may account for the increased accumulation of amide-containing compounds, sugars, and other metabolites detected in long-term bioconversion. Finally, the extracts recovered from the microbial degradation of hemp residues were able to support the growth of the yeast <i>Cryptococcus phenolicus</i> and the bacterium <i>Peribacillus simplex</i> as the sole source of nitrogen, paving the way for their potential use as biostimulants.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Could the Microbial Profiling of Normal Pancreatic Tissue from Healthy Organ Donors Contribute to Understanding the Intratumoral Microbiota Signature in Pancreatic Ductal Adenocarcinoma?","authors":"Francesca Tavano, Alessandro Napoli, Domenica Gioffreda, Orazio Palmieri, Tiziana Latiano, Matteo Tardio, Fabio Francesco di Mola, Tommaso Grottola, Markus W Büchler, Marco Gentile, Anna Latiano, Tommaso Mazza, Francesco Perri","doi":"10.3390/microorganisms13020452","DOIUrl":"10.3390/microorganisms13020452","url":null,"abstract":"<p><p>Pancreatic ductal adenocarcinoma (PDAC) is associated with intratumoral microbiota changes. However, defining the normal pancreatic microbial composition remains a challenge. Herein, we tested the hypothesis that the microbial profiling of normal pancreatic tissue from healthy organ donors (HC) could help in determining the signature of microbiota in PDAC. Matched pairs of tumor and normal tissues from PDAC patients (n = 32) and normal pancreatic tissues from HC (n = 17) were analyzed by 16S rRNA gene sequencing. Dissimilarities in all the beta metrics emerged in both normal samples and tumor samples, compared to HC (Bray-Curtis dissimilarity and Jaccard distance: <i>p</i> = 0.002; weighted UniFrac distances: <i>p</i> = 0.42 and <i>p</i> = 0.012, respectively; unweighted UniFrac distance: <i>p</i> = 0.009); a trend toward a lower Faith's phylogenetic distance was found at the tumor level vs. HC (<i>p</i> = 0.08). Within PDAC, a lower Faith's phylogenetic distance (<i>p</i> = 0.003) and a significant unweighted UniFrac distance (<i>p</i> = 0.024) were observed in tumor samples vs. normal samples. We noted the presence of a decreased abundance of bacteria with potential beneficial effects (<i>Jeotgalicoccus</i>) and anticancer activity (<i>Acinetobacter_guillouiae</i>) in PDAC vs. HC; bacteria involved in immune homeostasis and suppression of tumor progression (<i>Streptococcus_salivarius</i>, <i>Sphingomonas</i>) were reduced, and those implicated in tumor initiation and development (<i>Methylobacterium-Methylorubrum</i>, <i>g_Delftia</i>) were enhanced in tumor samples vs. normal samples. Metagenomic functions involved in fatty acid synthesis were reduced in normal samples compared to HC, while peptidoglycan biosynthesis IV and L-rhamnose degradation were more abundant in tumor samples vs. normal samples. Future prospective studies on larger populations, also including patients in advanced tumor stages and considering all potential existing confounding factors, as well as further functional investigations, are needed to prove the role of microbiota-mediated pathogenicity in PDAC.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HNP-1: From Structure to Application Thanks to Multifaceted Functions.","authors":"Jiaqi Zhang, Zhaoke Liu, Zhihao Zhou, Zile Huang, Yifan Yang, Junzhu Wu, Yanhong Liu","doi":"10.3390/microorganisms13020458","DOIUrl":"10.3390/microorganisms13020458","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are critical components of innate immunity in animals and plants, exhibiting thrilling prospectives as alternatives to traditional antibiotics due to their ability to combat pathogens without leading to resistance. Among these, Human Neutrophil Peptide-1 (HNP-1), primarily produced by human neutrophils, exhibits broad-spectrum antimicrobial activity against bacteria and viruses. However, the clinical application of HNP-1 has been hampered by challenges associated with mass production and inconsistent understanding of its bactericidal mechanisms. This review explores the structure and function of HNP-1, discussing its gene expression, distribution, immune functions and the regulatory elements controlling its production, alongside insights into its antimicrobial mechanisms and potential clinical applications as an antimicrobial agent. Furthermore, the review highlights the biosynthesis of HNP-1 using microbial systems as a cost-effective alternative to human extraction and recent studies revealing HNP-1's endogenous bactericidal mechanism. A comprehensive understanding of HNP-1's working mechanisms and production methods will pave the way for its effective clinical utilization in combating antibiotic-resistant infections.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and Anti-Inflammatory Effects of <i>Akkermansia muciniphila</i>-Derived Extracellular Vesicles.","authors":"Sasa Zhao, Jie Xiang, Minhazul Abedin, Jingyi Wang, Zhiwen Zhang, Zhongwei Zhang, Hua Wu, Junsong Xiao","doi":"10.3390/microorganisms13020464","DOIUrl":"10.3390/microorganisms13020464","url":null,"abstract":"<p><p>Bacterial extracellular vesicles (EVs) play a pivotal role in host-microbe communication. <i>Akkermansia muciniphila</i>, a symbiotic bacterium essential for intestinal health, is hypothesized to exert its effects via EVs. Here, we successfully isolated and characterized EVs derived from <i>A. muciniphila</i> (Am-EVs) using ultracentrifugation. Am-EVs exhibited a double-membrane structure, with an average diameter of 92.48 ± 0.28 nm and a proteomic profile comprising 850 proteins. In an in vitro model of lipopolysaccharide (LPS)-induced inflammation in human colorectal adenocarcinoma cells (Caco-2), treatment with both 25 and 50 μg/mL Am-EVs significantly reduced oxidative stress markers, including reactive oxygen species (ROS), nitric oxide (NO), and malondialdehyde (MDA), while restoring catalase activity (CAT). Am-EVs also suppressed the expression of pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). Subsequent transcriptomic sequencing and Western blot experiments revealed that Am-EVs attenuate the MAPK signaling pathway by downregulating TRIF, MyD88, p38 MAPK, and FOS while upregulating TGFBR2. These findings suggest that Am-EVs mediate anti-inflammatory effects through modulation of MAPK signaling, highlighting their potential as therapeutic agents in intestinal inflammation.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiofilm Effects of Plant Extracts Against <i>Staphylococcus aureus</i>.","authors":"Alexia Barbarossa, Antonio Rosato, Roberta Tardugno, Antonio Carrieri, Filomena Corbo, Francesco Limongelli, Luciana Fumarola, Giuseppe Fracchiolla, Alessia Carocci","doi":"10.3390/microorganisms13020454","DOIUrl":"10.3390/microorganisms13020454","url":null,"abstract":"<p><p>The global rise in antimicrobial resistance poses a significant threat to public health, necessitating alternative therapeutic options. One critical challenge is treating infections caused by biofilm-forming bacteria, which are notably resistant to conventional antibiotics. <i>Staphylococcus aureus</i>, including methicillin-resistant strains (MRSA), is a major pathogen in biofilm-related infections, complicating treatment and leading to chronic cases. Plant extracts have emerged as promising alternatives, offering new avenues for effective treatment. This study evaluated the antibacterial and antibiofilm activities of commercial extracts of <i>Vitis vinifera</i> L. (grape), <i>Camellia sinensis</i> L. (green tea), <i>Olea europaea</i> L. (olive), <i>Quercus robur</i> (oak), and <i>Coffea arabica</i> L. (coffee) against <i>S. aureus</i> strains from ATCC collections and clinical isolates. Preliminary screening using the disk diffusion test assessed the zones of inhibition, which was followed by minimum inhibitory concentration (MIC) determination via broth microdilution, with <i>Quercus robur</i> L. showing the best overall MIC results. The results obtained demonstrate the strong antibacterial activity of the extracts, with the MIC values ranging from 0.2 to 12.4 mg/mL. Using the XTT reduction assay, the extracts inhibited biofilm growth by 80-85% after 24 h of incubation, with <i>Coffea arabica</i> L. achieving interesting antibiofilm activities. These findings suggest that the investigated plant extracts hold potential as antimicrobial agents and biofilm inhibitors, offering an alternative approach to tackling antimicrobial resistance. Further research is needed to explore their potential applications in developing novel adjuvant therapies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Soil Microbial Composition in Rhizospheres Between Wilt Disease-Resistant and Susceptible Melon Varieties.","authors":"Lulu Qiu, Yu Zhu, Xinni Li, Yuchen Qin, Guifen Li, Yunfeng Ye, Yi He, Jinyan Huang, Shangdong Yang","doi":"10.3390/microorganisms13020444","DOIUrl":"10.3390/microorganisms13020444","url":null,"abstract":"<p><p>To screen out the bio-control soil microorganisms for preventing melon wilt, soil microbial compositions in rhizospheres between wilt-resistant and susceptible melon varieties were analyzed. The results showed that the soil fungal richness in rhizospheres of wilt-resistant melon varieties (MT) was significantly higher than that of wilt-susceptible melon varieties (MS). Additionally, in comparison with MS, soil bacterial compositions, such as Proteobacteria, Bacteroidota, <i>Acidibacter, Streptomyces</i>, etc., and the soil fungal compositions, such as <i>Penicillium</i>, <i>Derxomyces</i>, <i>Aspergillus</i>, and <i>Talaromyces</i>, enriched; also, <i>Trichoderma</i>, <i>Gibellulopsis</i>, and <i>Pseudallescheria</i> decreased in rhizospheres of wilt-resistant melon varieties (MT). Moreover, <i>Mycothermus</i>, <i>Zopfiella</i>, and <i>Cladorrhinum</i> were the unique soil-dominant fungal genera in rhizospheres of MT. All the above results suggested that the soil bacterial communities, such as Proteobacteria, Bacteroidota, Acidibacter, <i>Streptomyces</i>, etc., and the soil fungal communities, such as <i>Penicillium</i>, <i>Derxomyces</i>, <i>Aspergillus</i>, <i>Talaromyces Mycothermus</i>, <i>Zopfiella</i>, and <i>Cladorrhinum</i>, could be speculated as the potential soil bio-control microorganisms for preventing melon wilt.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration in Tracheal Morphology and Transcriptomic Features in Calves After Infection with <i>Mycoplasma bovis</i>.","authors":"Fan Liu, Fei Yang, Lei Guo, Mengmeng Yang, Yong Li, Jidong Li, Yanan Guo, Shenghu He","doi":"10.3390/microorganisms13020442","DOIUrl":"10.3390/microorganisms13020442","url":null,"abstract":"<p><p><i>Mycoplasma bovis</i> is one of the most important pathogens in animal husbandry, and the current infection and morbidity rates are increasing year by year, causing great losses to the farming industry and seriously affecting animal welfare. In this study, we took tracheal tissues from calves infected with <i>M. bovis</i> to make pathological tissue sections for observation, and selected tracheal tissues for transcriptome sequencing to screen differentially expressed genes based on the threshold |log2FoldChange| > 1 and Padjust < 0.05 and functional enrichment, to explore in depth the potential mechanisms of bovine tracheal damage caused by bovine tracheitis. Experiments were conducted to observe the changes in tracheal tissues after <i>M. bovis</i> infection through pathological sections of the trachea of <i>M. bovis</i>-infected calves. From the transcriptome sequencing results, we mined the main differential genes and important metabolic pathways of <i>M. bovis</i> causing damage to the trachea of calves. It was found that the cricoid cartilage tissue of the trachea was congested and hemorrhagic after <i>M. bovis</i> infection in calves, and the pathological sections showed localized necrosis of epithelial cells, disorganization, high inflammatory cell infiltration in the interepithelial and lamina propria, and some epithelial cell detachment. Transcriptome sequencing identified 4199 DEGs, including 1378 up-regulated genes and 2821 down-regulated genes. KEGG enrichment analysis indicated that the differential genes were enriched to 59 significantly differing signaling pathways, and a number of important metabolic pathways related to tracheitis induced by <i>M. bovis</i>-infected calves were unearthed. The major ones included IL-17, the Toll-like receptor, JAK/STAT, the PI3K-Akt signaling pathway, etc. In this study, we found that <i>M. bovis</i> infection of calves caused inflammatory damage to the trachea, and transcriptome sequencing results also showed significant differences in the expression of key genes such as IL-6 inflammatory factor, CASP8, and APOA1.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral Infection and Dissemination Through the Lymphatic System.","authors":"Morgan E Brisse, Heather D Hickman","doi":"10.3390/microorganisms13020443","DOIUrl":"10.3390/microorganisms13020443","url":null,"abstract":"<p><p>Many viruses induce viremia (virus in the blood) and disseminate throughout the body via the bloodstream from the initial infection site. However, viruses must often pass through the lymphatic system to reach the blood. The lymphatic system comprises a network of vessels distinct from blood vessels, along with interconnected lymph nodes (LNs). The complex network has become increasingly appreciated as a crucial host factor that contributes to both the spread and control of viral infections. Viruses can enter the lymphatics as free virions or along with migratory cells. Once virions arrive in the LN, sinus-resident macrophages remove infectious virus from the lymph. Depending on the virus, macrophages can eliminate infection or propagate the virus. A virus released from an LN is eventually deposited into the blood. This unique pathway highlights LNs as targets for viral infection control and for modulation of antiviral response development. Here, we review the lymphatic system and viruses that disseminate through this network. We discuss infection of the LN, the generation of adaptive antiviral immunity, and current knowledge of protection within the infected node. We conclude by sharing insights from ongoing efforts to optimize lymphatic targeting by vaccines and pharmaceuticals. Understanding the lymphatic system's role during viral infection enhances our knowledge of antiviral immunity and virus-host interactions and reveals potential targets for next-generation therapies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}