Microorganisms最新文献

{"title":"<i>Faecalibacterium prausnitzii</i> Is Associated with Disease Severity in MASLD but Its Supplementation Does Not Improve Diet-Induced Steatohepatitis in Mice.","authors":"Eliane Münte, Greta Viebahn, Amit Khurana, Jumpei Fujiki, Tomohiro Nakamura, Sonja Lang, Münevver Demir, Bernd Schnabl, Phillipp Hartmann","doi":"10.3390/microorganisms13030675","DOIUrl":"10.3390/microorganisms13030675","url":null,"abstract":"<p><p>The gut microbiota plays an important role in the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD). In this study, we aimed to evaluate the role of the butyrate-producing bacterium <i>Faecalibacterium prausnitzii</i> in MASLD and whether supplementation with butyrate-producing bacteria, in particular <i>Faecalibacterium prausnitzii</i>, can ameliorate diet-induced steatohepatitis in mice. The relative abundance of the genus <i>Faecalibacterium</i> and its most abundant strain <i>Faecalibacterium prausnitzii</i> was determined by 16S rRNA sequencing and quantitative polymerase chain reaction (qPCR), respectively, in 95 participants with MASLD and 19 healthy control subjects. Butyrate and butyrate-producing bacteria (<i>Faecalibacterium prausnitzii</i> and <i>Coprococcus comes</i>) were gavaged to C57BL/6 mice fed a steatohepatitis-inducing diet. The fecal relative abundance of <i>Faecalibacterium</i> and <i>Faecalibacterium prausnitzii</i> was decreased in subjects with MASLD versus healthy controls and lower in individuals with MASLD and stage 3-4 fibrosis versus those with stage 0-2 fibrosis. Sodium-butyrate supplementation improved hepatic steatosis in mice on high-fat diet (HFD). Gavage of various butyrate-producing bacteria including <i>Faecalibacterium prausnitzii</i> and <i>Coprococcus comes</i> isolated from humans did not improve HFD-induced liver disease in mice. Although the abundance of <i>Faecalibacterium prausnitzii</i> is associated with MASLD severity in humans, its gavage to mice does not improve experimental diet-induced liver disease.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Distribution in the Glacier Borehole Meltwater on the Eastern Broknes Peninsula of the Larsemann Hills and Adjacent Lake Water, East Antarctica.","authors":"Hongpeng Cui, Jibin Han, Bing Li, Youhong Sun, Da Gong, Xiaopeng Fan, Talalay Pavel, Dayi Zhang, Liang Gao, Hongchen Jiang","doi":"10.3390/microorganisms13030679","DOIUrl":"10.3390/microorganisms13030679","url":null,"abstract":"<p><p>The distribution and assembly mechanisms of microorganisms in Antarctic lakes and glaciers remain poorly understood, despite their ecological significance. This study investigates the bacterial diversity and community composition in glacier borehole meltwater samples from the eastern Broknes Peninsula of the Larsemann Hills and adjacent lake water samples in East Antarctica using high-throughput 16S rRNA gene sequencing. The results show that bacterial diversity in glacier borehole meltwater increased with depth, but remained lower than in lake water. Significant compositional differences were observed between lake and glacier borehole bacterial communities, with higher relative abundances of <i>Actinobacteria</i>, <i>Bacteroidia</i>, <i>Cyanobacteriia,</i> and <i>Verrucomicrobiae</i> in glacier borehole water samples, while <i>Alphaproteobacteria</i>, <i>Gammaproteobacteria</i>, <i>OLB14</i> (phylum <i>Chloroflexi</i>), <i>Acidimicrobiia</i>, and <i>Thermoleophilia</i> were more abundant in lake samples. These differences were attributed to distinct community assembly mechanisms: stochastic processes (ecological drift and dispersal limitation) dominated in lakes, while both stochastic (ecological drift and homogeneous dispersal) and deterministic (homogeneous selection) processes played key roles in glacier boreholes. This study enhances our understanding of bacterial community assembly and distribution patterns in Antarctic glacier ecosystems, providing insights into microbial biodiversity and biogeochemical cycling in these extreme environments.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Lignocellulose Degradation and Mycotoxin Reduction in Co-Composting with Bacterial Inoculation.","authors":"Cheng Chen, Xiaolong Tang, Chaosheng Liao, Xiaokang Huang, Mingjie Zhang, Yubo Zhang, Pan Wang, Siqi Yang, Ping Li, Chao Chen","doi":"10.3390/microorganisms13030677","DOIUrl":"10.3390/microorganisms13030677","url":null,"abstract":"<p><p>The burgeoning global silage industry has precipitated challenges related to the sustainable utilization of mycotoxin-contaminated silage. To understand the effect of bio-enhancement on lignocellulose degradation and mycotoxin reduction, mycotoxin-contaminated silage and rape straw were co-composted without (CK) or with different bacterial agents and their combinations. Compared to CK, the inoculation of <i>Weissella paramesenteroides</i> and <i>Bacillus subtilis</i> could increase the degradation rate of cellulose by 39.24% and lignin by 22.31% after composting. Inoculation of <i>W. paramesenteroides</i> and <i>Paenibacillus</i> sp. significantly enhanced cellulose and lignin degradation rates by 26.75% and 15.48%, respectively. Furthermore, this treatment significantly reduced mycotoxin levels (<i>p</i> < 0.05), including Aflatoxin B1 (AFB1, 64.48% reduction), T-2 toxin (65.02%), Ochratoxin A (OTA, 61.30%), Zearalenone (ZEN, 67.67%), and Vomitoxin (DON, 48.33%). Inoculation with <i>Paenibacillus</i> sp. and other bacteria increased total nitrogen by 48.34-65.52% through enhancing microbiological activity. Therefore, <i>Paenibacillus</i> sp. in combination with other bacteria could increase compost efficiency and reduce mycotoxin presence for better and safer utilization of agricultural waste by-products, enabling faster conversion of contaminated silage into safe soil amendments, which could reduce agricultural waste management costs.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline Characteristics of Bronchial Secretions and Bronchoalveolar Lavage Fluid in Patients with Ventilator-Associated Pneumonia.","authors":"Rodopi Stamatiou, Efrosyni Gerovasileiou, Maria Angeli, Konstantina Deskata, Vasiliki Tsolaki, Konstantinos Mantzarlis, Epameinondas Zakynthinos, Demosthenes Makris","doi":"10.3390/microorganisms13030676","DOIUrl":"10.3390/microorganisms13030676","url":null,"abstract":"<p><p>Mechanically ventilated (MV) patients often develop ventilator-associated pneumonia (VAP) with increased mortality risk, especially in VAP caused by multidrug-resistant (MDR) microorganisms. We evaluated MV patients and monitored VAP presentation, microbiologically confirmed. The patients underwent bronchoalveolar lavage (BAL) and blind bronchial aspiration (AC) at baseline. Systematic bronchial secretion and radiologic assessments were performed daily. The patients were classified as MDR-VAP, non-MDR-VAP, or non-VAP. The APACHE II and SOFA scores, microbiology, inflammatory markers, respiratory system characteristics, and ventilator settings were evaluated. BAL and AC were assessed for total protein levels, cellular number and profile, and IL-1β and TNF-α levels. Of the VAP patients, 46.1% presented with MDR-VAP due to <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, or <i>Stenotrophomonas maltophilia</i>, and 53.8%-with non-MDR-VAP. The VAP patients had higher APACHE II scores and airway pressure but a lower baseline PO₂/FIO₂ compared to the non-VAP patients, while PO₂/FIO₂ was increased in MDR-VAP compared to non-MDR-VAP. BAL protein, IL-1β, and cellular levels were increased in VAP vs. non-VAP and in non-MDR-VAP compared to MDR-VAP. Macrophages and polymorphonuclears were 34.36% and 23.76% in VAP, statistically significant increased compared to non-VAP. Their percentages were also increased in non-MDR-VAP compared to MDR-VAP. These differences imply a different immunological profile in non-MDR-VAP patients. In conclusion, MDR-VAP patients may present significant differences in baseline clinical characteristics and molecular biomarkers, which may help in prompt diagnosis and an improved therapeutic approach.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Metagenomic Next-Generation Sequencing, Sanger Sequencing, and Conventional Culture for Detecting Common Pathogens Causing Lower Respiratory Tract Infections in Clinical Samples.","authors":"Qiaolian Yi, Ge Zhang, Tong Wang, Jin Li, Wei Kang, Jingjia Zhang, Yali Liu, Yingchun Xu","doi":"10.3390/microorganisms13030682","DOIUrl":"10.3390/microorganisms13030682","url":null,"abstract":"<p><p>Metagenomic next-generation sequencing (mNGS) has emerged as a revolutionary tool for infectious disease diagnostics. The necessity of mNGS in real-world clinical practice for common Lower Respiratory Tract Infections (LRTI) needs further evaluation. A total of 184 bronchoalveolar lavage fluid (BALF) samples and 322 sputa associated with LRTI were fully examined. The detection performance was compared between mNGS and standard microbiology culture, using Sanger sequencing as the reference method. 52.05% (165/317) of sputa showed identical results for all three methods. Compared to Sanger sequencing, the same results obtained by mNGS were 88.20% (284/322). In 2.80% (9/322) of cases, Sanger sequencing detected more microorganisms, while mNGS detected more in 9% (29/322) of cases. For BALF, 49.41% (85/172) of cases showed identical results for all three methods. In 91.30% (168/184) of cases, identical results were produced by both mNGS and Sanger sequencing. mNGS detected more species in 7.61% (14/184) of cases, whereas in 2.80% (2/184) instances, the Sanger sequencing detected more microorganisms than mNGS. In the 184 BALF samples, 66 samples were identified as having co-infections by mNGS, Sanger sequencing identified 64 samples, and cultures identified 22 samples. Our study demonstrates that mNGS offers a significant advantage over conventional culture methods in detecting co-infections. For common bacterial pathogens, conventional culture methods are sufficient for detection. However, mNGS provides comprehensive pathogen detection and is particularly useful for identifying rare and difficult-to-culture pathogens.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Axillary Actinomycosis: A Case Report on the Integration of Culture and Molecular Diagnostics for Accurate Diagnosis of Polymicrobial Infections.","authors":"Junko Tezuka, Noriyuki Abe, Hiroshi Tanabe","doi":"10.3390/microorganisms13030671","DOIUrl":"10.3390/microorganisms13030671","url":null,"abstract":"<p><p>Actinomycosis is a chronic suppurative granulomatous disease caused by <i>Actinomyces</i> spp. Although cutaneous actinomycosis is rare, dermatologists must consider it due to its potential coexistence with other pathogens, often as part of polymicrobial infections. We present a rare case of primary axillary cutaneous actinomycosis in a young woman, likely triggered by cosmetic axillary hair removal and home shaving practices. Histological examination revealed characteristics of actinomycosis, including sulfur granules and Gram-positive filamentous structures. Bacterial cultures failed to isolate <i>Actinomyces</i>, but identified <i>Staphylococcus epidermidis</i>, <i>S. aureus</i> (MRSA), and <i>Corynebacterium simulans</i>, suggesting a polymicrobial infection contributing to the inflammatory response. Molecular analysis of DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue yielded a 675 bp PCR product using <i>Actinomyces</i>-specific primers. BLAST analysis confirmed the species as <i>A. gerencseriae</i>, establishing the diagnosis of actinomycosis. However, a 1000 bp PCR product obtained using universal 16S rDNA primers could not be sequenced successfully, likely due to the presence of multiple bacterial species. Notably, <i>Actinomyces</i> was detected only through molecular methods, while bacterial cultures identified the aforementioned bacteria. This discrepancy between FFPE-PCR results and bacterial culture findings demonstrates a key challenge in the microbiological diagnosis of polymicrobial infections. This case highlights the importance of integrating histopathological, microbiological, and molecular techniques for accurate pathogen identification in polymicrobial infections. The failure to detect <i>Actinomyces</i> in bacterial cultures, despite its presence in FFPE-PCR, suggests that conventional culture methods alone may be insufficient for diagnosing such infections. Extended culture durations, selective anaerobic culture techniques, and molecular diagnostic methods are essential for a comprehensive evaluation. Recognizing <i>Actinomyces</i> as more than a contaminant is important for timely diagnosis and effective treatment. Increased awareness of its potential involvement in polymicrobial infection should improve clinical outcomes.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and Antimicrobial Resistance Trends in Bacterial Keratitis: A Hospital-Based 10-Year Study (2014-2024).","authors":"Qingquan Shi, Deshuo Mao, Zijun Zhang, Ahyan Ilman Qudsi, Mingda Wei, Zhen Cheng, Yang Zhang, Zhiqun Wang, Kexin Chen, Xizhan Xu, Xinxin Lu, Qingfeng Liang","doi":"10.3390/microorganisms13030670","DOIUrl":"10.3390/microorganisms13030670","url":null,"abstract":"<p><p>Bacterial keratitis (BK) is a severe ocular infection that can lead to vision loss, with antimicrobial resistance (AMR) posing a growing challenge. This study retrospectively analyzed 1071 bacterial isolates from corneal infections over a 10-year period (2014-2024) at a tertiary ophthalmic center in Beijing, categorizing them into three distinct phases: pre-COVID-19, during COVID-19, and post-COVID-19. The results indicated significant changes in pathogen distribution, including a marked decrease in Gram-positive cocci (from 69.8% pre-COVID-19 to 49.3% in post-COVID-19, <i>p</i> < 0.001), particularly in <i>Staphylococcus epidermidis</i>. In contrast, Gram-positive bacilli, particularly <i>Corynebacterium</i> spp., increased from 4.2% to 16.1% (<i>p</i> < 0.001). The susceptibility to gatifloxacin, moxifloxacin, and ciprofloxacin significantly declined in both Gram-positive cocci and bacilli during the COVID-19 period (all <i>p</i> < 0.01). Gatifloxacin resistance in <i>Staphylococcus</i> rose from pre-COVID-19 (15.2%) to COVID-19 (32.7%), remaining high post-COVID-19 (29.7%). A similar trend was observed in <i>Streptococcus</i> and <i>Corynebacterium</i>, where resistance rose sharply from 12.0% and 22.2% pre-COVID-19 to 42.9% during COVID-19, and remained elevated at 40.0% and 46.4% post-COVID-19, respectively (<i>p</i> < 0.01). These findings emphasize the rapid rise of fluoroquinolone resistance in several bacterial groups, underscoring the urgent need for continuous surveillance and improved antimicrobial stewardship to enhance treatment outcomes.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Status and Trends of Gut Microbiota and Intestinal Diseases Based on Bibliometrics.","authors":"Xiao Sun, Jiancheng Zhai","doi":"10.3390/microorganisms13030673","DOIUrl":"10.3390/microorganisms13030673","url":null,"abstract":"<p><p>Gut microbiota plays an important role in gut health, and its dysbiosis is closely related to the pathogenesis of various intestinal diseases. The field of gut microbiota and intestinal diseases has not yet been systematically quantified through bibliometric methods. This study conducted bibliometric analysis to delineate the evolution of research on gut microbiota and intestinal diseases. Data were sourced from the Web of Science Core Collection database from 2009 to 2023 and were scientometrically analyzed using CiteSpace. We have found that the number of annual publications has been steadily increasing and showing an upward trend. China and the Chinese Academy of Sciences are the country and institution with the most contributions, respectively. <i>Frontiers in Microbiology</i> and <i>Nutrients</i> are the journals with the most publications, while <i>Plos One</i> and <i>Nature</i> are the journals with the most citations. The field has shifted from focusing on traditional descriptive analysis of gut microbiota composition to exploring the causal relationship between gut microbiota and intestinal diseases. The research hotspots and trends mainly include the correlation between specific intestinal diseases and gut microbiota diversity, the mechanism of gut microbiota involvement in intestinal diseases, the exploration of important gut microbiota related to intestinal diseases, and the relationship between gut microbiota and human gut health. This study provides a comprehensive knowledge map of gut microbiota and intestinal diseases, highlights key research areas, and outlines potential future directions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Succession of Microbiome Communities in the Early Establishing Process of an Epilithic Algal Matrix in a Fringing Reef.","authors":"Beiye Zhang, Simin Hu, Chen Zhang, Tiancheng Zhou, Tao Li, Hui Huang, Sheng Liu","doi":"10.3390/microorganisms13030672","DOIUrl":"10.3390/microorganisms13030672","url":null,"abstract":"<p><p>An epilithic algal matrix (EAM) exhibits rapid expansion, recovery capacity, and high adaptability, leading to widespread distribution in degraded coral reef habitats. However, limited research on the dynamic processes of succession hinders a comprehensive understanding of EAM formation. To examine the influence of succession processes and environmental factors on the composition of EAM microbial communities, a three-factor (time × depth × attached substrate type) crossover experiment was conducted in the Luhuitou Reef Area, Sanya, China. Microbial community compositions were analyzed through 16S rRNA gene amplicon sequencing. The community was predominantly composed of proteobacteria (61.10-92.75%), cyanobacteria (2.47-23.54%), bacteroidetes (0.86-8.49%), and firmicutes (0.14-7.76%). Successional processes were found to significantly shape the EAM-associated microbial communities in the Luhuitou Reef Area. Proteobacteria played a crucial role in biofilm formation during this process, while cyanobacteria contributed to the structural complexity of microhabitats within the EAM. A chaotic aggregation stage of approximately one month was observed before transitioning into an expansion stage, eventually stabilizing into a low-diversity community. Although the relatively smooth substrate supported high biodiversity, microorganisms displayed no preference for the three different substrates. While no significant differences in community composition were observed at small-scale depths, cyanobacteria and bacteroidetes showed positive correlations with light and temperature, respectively. The EAM-associated microbial community exhibited higher complexity in the shallower regions under increased light intensity and temperature. Given the characteristics of the microbial community succession process, continuous monitoring of changes in microbial community structure and key taxa (such as proteobacteria and cyanobacteria) during EAM formation is recommended.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Newly Isolated <i>Rosenblumvirus</i> Phage Infecting <i>Staphylococcus aureus</i> from Different Sources.","authors":"Paloma Cavalcante Cunha, Pedro Samuel de Souza, Ana Julia Dill Rosseto, Isabella Ribeiro Rodrigues, Roberto Sousa Dias, Vinícius da Silva Duarte, Davide Porcellato, Cynthia Canêdo da Silva, Sérgio Oliveira de Paula","doi":"10.3390/microorganisms13030664","DOIUrl":"10.3390/microorganisms13030664","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is a globally significant pathogen associated with severe infections, foodborne illnesses, and animal diseases. Its control has become increasingly challenging due to the spread of antibiotic-resistant strains, highlighting the urgent need for effective alternatives. In this context, bacteriophages have emerged as promising biocontrol agents. This study aimed to characterize the newly isolated <i>Staphylococcus</i> phage CapO46 and evaluate its efficacy in reducing <i>S. aureus</i> in milk. Identified as a new species within the <i>Rosenblumvirus</i> genus, CapO46 exhibited a podovirus-like structure and a small linear dsDNA genome (17,107 bp), with no lysogeny-related, antimicrobial resistance, or virulence genes. Host range assays demonstrated its ability to infect all 31 <i>S. aureus</i> isolates from two different countries and in diverse environmental contexts, achieving high efficiency of plating (EOP > 0.5) in 64.5% of cases. Kinetic analyses revealed rapid adsorption and a short latent period, with a burst size of approximately 30 PFU/cell. In UHT whole-fat milk, CapO46 achieved a maximum reduction of 7.2 log10 CFU/mL in bacterial load after 12 h, maintaining significant suppression (1.6 log10 CFU/mL) after 48 h. Due to its genetic safety, high infectivity across multiple isolates, and antimicrobial activity in milk, CapO46 can be considered a promising candidate for <i>S. aureus</i> biocontrol applications.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}