Microorganisms最新文献_第4页

Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type Listeria monocytogenes, and Listeria innocua During a Real-Time Shelf-Life Study.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-15 DOI: 10.3390/microorganisms13030668

Ranju Kafle, Aliyar Cyrus Fouladkhah

{"title":"Impact of Nisin on Proliferation of Background Microbiota, Pressure-Stressed and Wild-Type Listeria monocytogenes, and Listeria innocua During a Real-Time Shelf-Life Study.","authors":"Ranju Kafle, Aliyar Cyrus Fouladkhah","doi":"10.3390/microorganisms13030668","DOIUrl":"10.3390/microorganisms13030668","url":null,"abstract":"With the rapid implementation of high-pressure processing in many sectors of the food industry, considerations associated with pressure-stressed microorganisms are emerging. Nisin was utilized in this study for controlling the proliferation of Listeria monocytogenes and L. innocua inoculated on cold-smoked trout during a 4-week refrigerated shelf-life trial. Wild-type and pressure-stressed phenotypes of Listeria were compared in this study. The pressure-stressed phenotypes were prepared by treating the surrogate strain and pathogen mixture at 103.4 MPa (15K PSI) for 20 min. L. monocytogenes multiplied extensively during the 4-week refrigerated trial and counts were increased (p < 0.05) from 3.68 ± 0.1 log CFU/g on the first week to 6.03 ± 0.1 log CFU/g. Both phenotypes and the surrogate microorganisms illustrated similar (p ≥ 0.05) multiplication trends. Unlike samples subjected to water treatment, nisin was effective (p < 0.05) in keeping the microbial counts lower compared with the controls, particularly earlier during the shelf-life trial. Our study illustrates that the selected surrogate microorganism has comparable sensitivity to nisin relative to L. monocytogenes and thus could be used interchangeably in future public health microbiology challenge studies with similar scope. Additionally, we observed that pressure-stressed L. monocytogenes has proliferation and sensitivity to nisin comparable to wild-type pathogen.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Advances in Leptospirosis: A Comparative Analysis of Paraclinical Tests with a Focus on PCR.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-15 DOI: 10.3390/microorganisms13030667

Elena Ciurariu, Catalin Prodan-Barbulescu, Diana-Maria Mateescu, Paul Tutac, Virgiliu-Bogdan Sorop, Monica Susan, Norberth-Istvan Varga

{"title":"Diagnostic Advances in Leptospirosis: A Comparative Analysis of Paraclinical Tests with a Focus on PCR.","authors":"Elena Ciurariu, Catalin Prodan-Barbulescu, Diana-Maria Mateescu, Paul Tutac, Virgiliu-Bogdan Sorop, Monica Susan, Norberth-Istvan Varga","doi":"10.3390/microorganisms13030667","DOIUrl":"10.3390/microorganisms13030667","url":null,"abstract":"Leptospirosis is a zoonotic disease with a varied clinical presentation that can mimic other infectious diseases, posing diagnostic challenges. While the Microscopic Agglutination Test (MAT) remains the gold standard for serological diagnosis, its limitations have led to increasing interest in polymerase chain reaction (PCR) as a rapid and sensitive diagnostic tool. This systematic review evaluates the role and clinical applications of PCR for diagnosing human leptospirosis. We analyzed the sensitivity and specificity of PCR, compared its performance with other diagnostic tests, and assessed the comparative utility of blood and urine samples for PCR testing. Our findings demonstrate that PCR has a high sensitivity and specificity, particularly in the early stages of the disease. Combining PCR with serological tests like MAT can maximize the diagnostic accuracy across different stages of illness. We recommend that PCR be considered a first-line diagnostic test for suspected leptospirosis, especially when rapid diagnosis is crucial. Further research is needed to standardize PCR protocols and explore its potential in differentiating Leptospira species and serotypes. By leveraging the strengths of PCR and combining it with other diagnostic methods, we can significantly improve the diagnosis and management of leptospirosis.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Perspective on Weissella confusa as a Promising Biocontrol Agent Against Fusaria.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-15 DOI: 10.3390/microorganisms13030666

Sukumaran Vipin Krishnan, Prakasan A Anaswara, Kesavan Madhavan Nampoothiri, Szilvia Kovács, Cintia Adácsi, Ida Miklós, Szabina Király, István Pócsi, Tünde Pusztahelyi

{"title":"Unveiling the Perspective on Weissella confusa as a Promising Biocontrol Agent Against Fusaria.","authors":"Sukumaran Vipin Krishnan, Prakasan A Anaswara, Kesavan Madhavan Nampoothiri, Szilvia Kovács, Cintia Adácsi, Ida Miklós, Szabina Király, István Pócsi, Tünde Pusztahelyi","doi":"10.3390/microorganisms13030666","DOIUrl":"10.3390/microorganisms13030666","url":null,"abstract":"The biotechnological potential of the lactic acid bacterial genus Weissella has not been fully unearthed. Since Weissella have not been tested against Fusaria and their mycotoxins, newly isolated Weissella confusa strains were characterized and tested for their antifungal capacities on Fusarium plant pathogens. W. confusa BF2 and ML2 successfully inhibited Fusarium verticillioides NCIM 1100, F. verticillioides NCIM 1099, Fusarium graminearum MTCC 2089, and Fusarium oxysporum MTCC 284 in co-cultures. Ethyl acetate extracts of the cell-free culture supernatants (CFCS) of W. confusa also exhibited antifungal activity against the tested fungal cultures. The main mycotoxins of Fusaria were tested against the Weissella isolates. In MRS broth, W. confusa BF2 was resistant to the Fusarium mycotoxins (deoxynivalenol, zearalenone, T2, and fumonisin B1), while the ML2 strain showed 22.1-24.5% growth inhibition. Meanwhile, neither bacterium showed potential in mycotoxin reduction. The study highlighted that W. confusa BF2 and ML2 and their CFCS are suitable for Fusarium growth inhibition, as shown on surface-sterilized peanuts and wheat grains, but not for mycotoxin elimination.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Antimicrobial Efficacy of Amine-Containing Surfactants Against Cysts and Trophozoites of Acanthamoeba spp.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-15 DOI: 10.3390/microorganisms13030665

Dharanga Ratnayake, Michael Ansah, Brian Batham, Daniel Keddie, Gavin McNee, Wayne Heaselgrave

{"title":"The Antimicrobial Efficacy of Amine-Containing Surfactants Against Cysts and Trophozoites of Acanthamoeba spp.","authors":"Dharanga Ratnayake, Michael Ansah, Brian Batham, Daniel Keddie, Gavin McNee, Wayne Heaselgrave","doi":"10.3390/microorganisms13030665","DOIUrl":"10.3390/microorganisms13030665","url":null,"abstract":"Microbial keratitis, a vision-threatening infection commonly linked to contact lens use, poses a significant challenge, particularly when caused by Acanthamoeba species. Acanthamoeba keratitis (AK) is difficult to treat due to the organism's ability to form resilient cysts, necessitating prolonged and complex therapeutic interventions. This study evaluated novel amidopropyl dimethylamines (APDs) and amidopropyl quaternary trimethylammoniums (APTs) for their antimicrobial efficacy against Acanthamoeba castellanii and Acanthamoeba polyphaga cysts. Minimum effective concentrations were determined, and time-kill assays assessed microbial inactivation over 24 h. The results indicated that certain APTs, particularly elaidamidopropyl trimethylammonium (EAPT) and oleamidopropyl trimethylammonium (OAPT), demonstrated superior cysticidal activity compared to the commercially used MAPD, achieving greater log reductions within 24 h (p < 0.0001) at a concentration of 25 µM. The enhanced efficacy of these compounds is potentially attributed to their unsaturated alkyl chains and positive charge, improving antimicrobial activity through the greater disruption of the Acanthamoeba cell membrane. These findings highlight the potential of APTs as alternative agents for incorporation into multipurpose lens disinfectants and AK treatment, offering improved disinfection efficacy. Further investigation is justified to optimise formulations for clinical and commercial applications.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Molnupiravir in Reducing the Risk of Severe Outcomes in Patients with SARS-CoV-2 Infection: A Real-Life Full-Matched Case-Control Study (SAVALO Study).

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-15 DOI: 10.3390/microorganisms13030669

Ivan Gentile, Riccardo Scotto, Maria Michela Scirocco, Francesco Di Brizzi, Federica Cuccurullo, Maria Silvitelli, Luigi Ametrano, Francesco Antimo Alfè, Daria Pietroluongo, Irene Irace, Mariarosaria Chiariello, Noemi De Felice, Simone Severino, Giulio Viceconte, Nicola Schiano Moriello, Alberto Enrico Maraolo, Antonio Riccardo Buonomo, Agnese Giaccone, On Behalf Of Federico Ii Covid Team

{"title":"Efficacy of Molnupiravir in Reducing the Risk of Severe Outcomes in Patients with SARS-CoV-2 Infection: A Real-Life Full-Matched Case-Control Study (SAVALO Study).","authors":"Ivan Gentile, Riccardo Scotto, Maria Michela Scirocco, Francesco Di Brizzi, Federica Cuccurullo, Maria Silvitelli, Luigi Ametrano, Francesco Antimo Alfè, Daria Pietroluongo, Irene Irace, Mariarosaria Chiariello, Noemi De Felice, Simone Severino, Giulio Viceconte, Nicola Schiano Moriello, Alberto Enrico Maraolo, Antonio Riccardo Buonomo, Agnese Giaccone, On Behalf Of Federico Ii Covid Team","doi":"10.3390/microorganisms13030669","DOIUrl":"10.3390/microorganisms13030669","url":null,"abstract":"We conducted a real-life case-control study among outpatients with Omicron SARS-CoV-2 infection to assess the effectiveness of molnupiravir (MNP) in reducing hospital admission, admission to the intensive care unit, and death at day 28. Cases were SARS-CoV-2-positive patients seeking medical care within five days of symptom onset from 1 January to 31 December 2022, who received MNP. Controls were selected from a regional database among positive subjects who did not receive antiviral treatment for SARS-CoV-2. A total of 1382 patients were included (146 cases, 1236 controls). Vaccinated patients had a lower risk of mortality and of the composite outcome (hospital admission, ICU admission, or all-cause death) than unvaccinated ones (0.6% vs. 7.8%, p < 0.001 and 2% vs. 7.8%, p = 0.001, respectively). After full-matching propensity score analysis, MNP-treated subjects had a lower incidence of the composite outcome, although no effect was observed on individual outcomes. In subgroup analyses by vaccination status, MNP was effective in preventing all outcomes among unvaccinated patients and reduced the risk of ICU admission in both vaccinated and unvaccinated patients. Molnupiravir treatment effectively reduced the composite outcome risk in outpatients with SARS-CoV-2 infection, with a more pronounced benefit in unvaccinated patients. These findings highlight MNP's potential to help prevent disease progression in high-risk patients, thereby supporting its role as an outpatient therapeutic option for COVID-19.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol Potential of Bacillus velezensis RS65 Against Phytophthora infestans: A Sustainable Strategy for Managing Tomato Late Blight.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-14 DOI: 10.3390/microorganisms13030656

Hasna Elhjouji, Redouan Qessaoui, Hafsa Houmairi, Khadija Dari, Bouchaib Bencharki, El Hassan Mayad, Hinde Aassila

{"title":"Biocontrol Potential of Bacillus velezensis RS65 Against Phytophthora infestans: A Sustainable Strategy for Managing Tomato Late Blight.","authors":"Hasna Elhjouji, Redouan Qessaoui, Hafsa Houmairi, Khadija Dari, Bouchaib Bencharki, El Hassan Mayad, Hinde Aassila","doi":"10.3390/microorganisms13030656","DOIUrl":"10.3390/microorganisms13030656","url":null,"abstract":"This study aimed to investigate the biocontrol activity of rhizosphere isolates against late blight disease of tomatoes caused by the fungus Phytophthora infestans. A total of 30 rhizospheric bacterial isolates were evaluated for their antagonistic activity against P. infestans in vitro and in vivo. The results demonstrated that among the 30 isolates tested, six (RS65, RP6, RS47, RS46, RP2, and RS61) exhibited a highly significant inhibitory effect (p < 0.001) on the mycelial growth of P. infestans in vitro, with the inhibition rate exceeding 67%. Among the isolates, RS65 exhibited the highest inhibition rate at 78.48%. For antagonistic mechanisms, the results demonstrated that the six isolates exhibited significant enzymatic activity, including proteolytic, lipolytic, and chitinolytic activity, as well as the production of HCN, cellulase, and pectinase. Isolate RS65, which showed the highest inhibition rate, was further evaluated under greenhouse conditions. This investigation revealed significant differences in the severity of late blight between the control and the RS65 treatment. The control showed a severity level of 31.26%, whereas the RS65 treatment achieved the lowest severity of 16.54%. Molecular identification results indicated that the RS65 isolate (accession numbers PV208381) is a Bacillus genus with 99% proximity to Bacillus velezensis. This finding suggests that the Bacillus RS65 treatment could provide effective protection against P. infestans infection in tomato plants. These findings highlight the potential of Bacillus RS65 as a biocontrol agent in integrated disease management for tomato late blight.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Fertilization Alleviates Microplastic Effects on Soil Protist Communities and Rape (Brassica napus L.) Growth.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-14 DOI: 10.3390/microorganisms13030657

Ge Wang, Maolu Wei, Qian Sun, Ting Shen, Miaomiao Xie, Dongyan Liu

{"title":"Nitrogen Fertilization Alleviates Microplastic Effects on Soil Protist Communities and Rape (Brassica napus L.) Growth.","authors":"Ge Wang, Maolu Wei, Qian Sun, Ting Shen, Miaomiao Xie, Dongyan Liu","doi":"10.3390/microorganisms13030657","DOIUrl":"10.3390/microorganisms13030657","url":null,"abstract":"Agricultural plastic mulch enhances crop yields but leads to persistent microplastic contamination in soils. Concurrently, nitrogen (N) fertilization and atmospheric deposition profoundly reshape microbial ecosystems. This study examined the individual and interactive effects of polyethylene microplastics (PE, 1% w/w) and nitrogen addition (N, 180 kg ha-1 yr-1) on soil protist communities and rape (Brassica napus L.) productivity. High-throughput sequencing and soil-plant trait analyses revealed that PE alone reduced the soil water retention and the rape biomass while elevating the soil total carbon content, C/N ratios, and NH₄⁺-N/NO₃--N levels. Conversely, N addition significantly boosted the rape biomass and the chlorophyll content, likely through enhanced nutrient availability. Strikingly, the combined PE_N treatment exhibited antagonistic interactions; protist diversity and functional group composition stabilized to resemble the control conditions, and the rape biomass under the PE_N treatment showed no difference from the CK (with basal fertilizer only), despite significant reductions under the PE treatment alone. Soil nutrient dynamics (e.g., the SWC and the C/N ratio) and the protist community structure collectively explained 96% of the biomass variation. These findings highlight the potential of nitrogen fertilization to mitigate microplastic-induced soil degradation, offering a pragmatic strategy to stabilize crop productivity in contaminated agricultural systems. This study underscores the importance of balancing nutrient management with pollution control to sustain soil health under global microplastic and nitrogen deposition pressures.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

McFarland Standards-Based Spectrophotometry Method for Calculating Approximate Multiplicity of Infection for an Obligate Intracellular Bacterium Anaplasma phagocytophilum.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-14 DOI: 10.3390/microorganisms13030662

P P Mahesh, Jaydeep Kolape, Hameeda Sultana, Girish Neelakanta

{"title":"McFarland Standards-Based Spectrophotometry Method for Calculating Approximate Multiplicity of Infection for an Obligate Intracellular Bacterium Anaplasma phagocytophilum.","authors":"P P Mahesh, Jaydeep Kolape, Hameeda Sultana, Girish Neelakanta","doi":"10.3390/microorganisms13030662","DOIUrl":"10.3390/microorganisms13030662","url":null,"abstract":"Anaplasma phagocytophilum is an obligate intracellular Gram-negative bacterium that causes human granulocytic anaplasmosis. Assessing the number of these bacteria is important for in vitro and in vivo infection studies. Colony count is used to set references for the multiplicity of infections in the case of culturable bacteria. However, the number of bacteria present inside the host cells, in which the bacteria are maintained, can be considered in the case of obligate intracellular bacteria. McFarland standards are a series of turbidity-based standards used to visually assess the approximate number of culturable bacteria. The turbidity of each standard can be related to their respective absorbances or optical densities (ODs). In this study, we describe a simple method to assess the approximate number of A. phagocytophilum based on McFarland standards. The ODs of cell-free crude extracts of A. phagocytophilum were used to assess the approximate number of bacteria while considering that the cell debris also contributes to the ODs. The consistency of this method was also tested using the bacterial cultures grown at different times. In summary, we provide a simple method to estimate the number of obligate intracellular bacteria for use in in vitro infection studies.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Feeding Fermented Cassava Leaves on Intestinal Morphology, Cecal Microbiota, and Metabolome in Hybrid Geese.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-14 DOI: 10.3390/microorganisms13030660

Mao Li, Tieshan Xu, Xuejuan Zi, Renlong Lv, Lihong Gu

{"title":"Effects of Feeding Fermented Cassava Leaves on Intestinal Morphology, Cecal Microbiota, and Metabolome in Hybrid Geese.","authors":"Mao Li, Tieshan Xu, Xuejuan Zi, Renlong Lv, Lihong Gu","doi":"10.3390/microorganisms13030660","DOIUrl":"10.3390/microorganisms13030660","url":null,"abstract":"In this study, we characterized the effects of a diet supplemented with fermented cassava leaves (FCLs) on growth performance, intestinal morphology, the cecal microbiota, and cecal metabolites in hybrid geese. We found that the FCL diet was beneficial to goose growth performance and also promoted a healthy intestinal morphology, as reflected by better morphology properties of the duodenum, jejunum, ileum, and cecum. Moreover, the FCL diet significantly altered cecal microbial diversity and composition, increasing the diversity and abundance of the beneficial Bacteroides. Further, the FCL diet increased the complexity and stability of cecal microbial co-occurrence network interactions as a result of altered topological distributions in the network, such as edges, density, degree, and betweenness. The FCL diet had clear impacts on the composition and abundance of cecal metabolites, with increases in metabolites involved in amino acid biosynthesis, digestion, and absorption, as well as an upregulation of associated metabolic pathways. Based on these benefits to growth performance, intestinal development, and cecal microbe-mediated metabolism in geese, FCLs can be utilized as a reliable feed resource for geese in tropical and subtropical regions.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection with Jujube Witches' Broom Phytoplasma Alters the Expression Pattern of the Argonaute Gene Family in Ziziphus jujuba.

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-03-14 DOI: 10.3390/microorganisms13030658

Jia Yao, Zesen Qiao, Ziming Jiang, Xueru Zhao, Ziyang You, Wenzhe Zhang, Jiancan Feng, Chenrui Gong, Jidong Li

{"title":"Infection with Jujube Witches' Broom Phytoplasma Alters the Expression Pattern of the Argonaute Gene Family in Ziziphus jujuba.","authors":"Jia Yao, Zesen Qiao, Ziming Jiang, Xueru Zhao, Ziyang You, Wenzhe Zhang, Jiancan Feng, Chenrui Gong, Jidong Li","doi":"10.3390/microorganisms13030658","DOIUrl":"10.3390/microorganisms13030658","url":null,"abstract":"The cultivation of jujube (Ziziphus jujuba) in China is threatened by jujube witches' broom (JWB) disease, a devastating infectious disease associated with JWB phytoplasma ('Candidatus Phytoplasma ziziphi'). In many plants, proteins in the Argonaute (AGO) family, as main components of the RNA-induced silencing complex (RISC), play important roles in RNA silencing and pathogen resistance. The jujube telomere-to-telomere genome was searched by BLAST using Arabidopsis AGOs as probes. A total of nine jujube AGO gene members were identified, with each containing the conserved N-terminal, PZA, and PIWI domains. Phylogenetic analysis revealed that the nine jujube AGOs scattered into all three Arabidopsis AGO clades. Expression patterns of the ZjAGO genes were analyzed in response to phytoplasma in transcriptome data and by qRT-PCR. The jujube-phytoplasma interaction altered the expression of jujube AGO genes. ZjAGO1 and ZjAGO8 were up-regulated in the majority of the eight sampling periods subjected to qRT-PCR analysis. In the transcriptome data, ZjAGO1 and ZjAGO8 were also up-regulated during the key stages 37 and 39 weeks after grafting (WAG) with phytoplasma-infected material. These two jujube Argonaute genes may play important roles in response to JWB phytoplasma infection.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0