Microorganisms最新文献

{"title":"Inhibitory Effects of Sulfur Derivatives on <i>Leishmania tarentolae</i> Cell Viability and Secreted Acid Phosphatase In Vitro.","authors":"Henry H Shang, Zaryna Z Zelaya, Christopher G Hamaker, Marjorie A Jones","doi":"10.3390/microorganisms12122641","DOIUrl":"https://doi.org/10.3390/microorganisms12122641","url":null,"abstract":"<p><p>Sulfonamide drugs were the original class of antibiotics, demonstrating the antibacterial potential of dithiocarbazate and thiosemicarbazone Schiff base derivatives of syringaldehyde and 4-hydroxy-3,5-dimethylbenzaldehyde. We synthesized unique Schiff bases via the condensation of the aldehydes with hydrazine derivatives, which allows for the easy synthesis of several related compounds. These Schiff base derivatives were tested for antileishmanial properties against the parasitic protozoan <i>Leishmania tarentolae</i>. The inhibitory properties of these sulfur compounds were tested using a series of cell viability and secreted acid phosphatase (SAP) assays. The results demonstrated that compounds ZZ1-04 and ZZ1-20 had potent inhibitory effects on parasite cell viability and SAP, an enzyme that may play a role in infectivity. These results increase our understanding of the role of sulfur in inhibiting <i>Leishmania</i>, providing more knowledge of the structural activity relationships that may prove critical for their development into possible antileishmanial treatments.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological Detection of Ovine Gammaherpesvirus 2 Antibodies in Dairy Farms from Southern Brazil.","authors":"Selwyn Arlington Headley, Dawn Marie Grant, Juliana Torres Tomazi Fritzen, Felippe Danyel Cardoso Martins, Stefany Lia Oliveira Camilo, Eloiza Teles Caldart, Júlio Augusto Naylor Lisbôa, Amauri Alcindo Alfieri, George Cameron Russell","doi":"10.3390/microorganisms12122629","DOIUrl":"https://doi.org/10.3390/microorganisms12122629","url":null,"abstract":"<p><p>Sheep-associated malignant catarrhal fever (SA-MCF) is a severe lymphoproliferative vascular disease of cattle that is caused by ovine gammaherpesvirus 2 (OvGHV2), which is a <i>Macavirus</i> within the <i>Gammaherpesvirinae</i> subfamily. SA-MCF occurs worldwide in several mammalian hosts. Alternatively, alcelaphine gammaherpesvirus 1 (AlGHV1) is a <i>Macavirus</i> that causes wildebeest-associated malignant catarrhal fever (MCF), which principally occurs in cattle from Africa. Previous serological assays to evaluate the presence of MCF in mammals used a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA). This CI-ELISA is based on the 15A antigenic epitope that is common to all <i>Macavirus</i> associated with the development of MCF in their respective hosts. This study evaluated an indirect MCF-specific ELISA assay based on the AlGHV1 C500 strain to detect antibodies against OvGHV2 in 43 closed dairy cattle farms from Southern Brazil. These farms are located in a region where subclinical infections by OvGHV2 have been detected in free-ranging wild boars (<i>Sus scrofa</i>). Sheep or goats were not reared at these farms or within the proximity of these farms. Risk factors associated with seropositivity to OvGHV2 were evaluated, while the possible participation of subclinically infected wild boars in the dissemination of OvGHV2 was estimated using spatial analysis. Sera from 29 dairy cows from 16 farms demonstrated sample/positive (S/P) values considered positive with this MCF-specific ELISA (cutoff S/P, 0.063). The S/P values for the positive dairy cows varied between 0.0633 and 0.2510 (mean, 0.0998; standard deviation, 0.0476). At least one cow was seropositive in 16/43 (37.2%) of these farms, with seropositivity identified in 29/367 (7.9%) of dairy cows maintained at these farms. Additionally, dairy cows raised within the intensive system had a more than threefold higher chance of being seropositive to OvGHV2 relative to those reared within the semi-intensive system. Furthermore, the spatial evaluation revealed that cows on dairy farms within a 50 km radius of the home range of subclinically infected wild boars had an increased risk of being seropositive to this assay. These findings demonstrated that the AlGHV1 C500-specific MCF ELISA can be efficiently used to monitor the occurrence of OvGHV2 in cattle. In addition, the occurrence of subclinically infected free-ranging wild boars within a radius of 50 km from susceptible cattle may be a possible risk factor for the occurrence of OvGHV2-related infections in these animals from Southern Brazil. These initial results are fundamental to understanding the epidemiology of OvGHV2-associated infections and clinical SA-MCF in mammals in Brazil.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mercury-Resistant Bacteria Isolated from an Estuarine Ecosystem with Detoxification Potential.","authors":"Marynes Quintero, Sol D Zuluaga-Valencia, Lady Giselle Ríos-López, Olga Sánchez, Cesar A Bernal, Niza Sepúlveda, Javier Gómez-León","doi":"10.3390/microorganisms12122631","DOIUrl":"https://doi.org/10.3390/microorganisms12122631","url":null,"abstract":"<p><p>Mercury pollution is a significant environmental issue, primarily resulting from industrial activities, including gold mining extraction. In this study, 333 microorganisms were tested in increasing mercury concentrations, where 158 bacteria and 14 fungi were able to grow and remain viable at concentrations over 5.0 mg/L of mercuric chloride (II). One of the bacterial strains, <i>Stenotrophomonas</i> sp. INV PRT0231, isolated from the mouth of the San Juan River in the Chocó region in Colombia, showed a high mercury resistance level (MIC₉₀ of 27 ± 9 mg/L), with a removal rate of 86.9%, an absorption rate of 1.2%, and a volatilization rate of 85.7% at pH 6.0 and 30.0 °C. The FTIR analysis showed changes in the functional groups, including fatty acid chains and methyl groups, proteins, and lipopolysaccharides associated with the carboxylate group (COO^-), suggesting an important role of these biomolecules and their associated functional groups as mechanisms employed by the bacterium for mercury detoxification. Our study contributes to the understanding of the mechanisms of mercury biotransformation in microbial environmental isolates to help develop bioremediation strategies to mitigate mercury pollution caused by anthropogenic activities.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome Structures and Beneficial Bacteria in Soybean Roots Under Field Conditions of Prolonged High Temperatures and Drought Stress.","authors":"Sandeep Gouli, Aqsa Majeed, Jinbao Liu, David Moseley, M Shahid Mukhtar, Jong Hyun Ham","doi":"10.3390/microorganisms12122630","DOIUrl":"https://doi.org/10.3390/microorganisms12122630","url":null,"abstract":"<p><p>Drought stress has a significant impact on agricultural productivity, affecting key crops such as soybeans, the second most widely cultivated crop in the United States. Endophytic and rhizospheric microbial diversity analyses were conducted with soybean plants cultivated during the 2023 growing season amid extreme weather conditions of prolonged high temperatures and drought in Louisiana. Specifically, surviving and non-surviving soybean plants were collected from two plots of a Louisiana soybean field severely damaged by extreme heat and drought conditions in 2023. Although no significant difference was observed between surviving and non-surviving plants in microbial diversity of the rhizosphere, obvious differences were found in the structure of the endophytic microbial community in root tissues between the two plant conditions. In particular, the bacterial genera belonging to Proteobacteria, <i>Pseudomonas</i> and <i>Pantoea</i>, were predominant in the surviving root tissues, while the bacterial genus <i>Streptomyces</i> was conspicuously dominant in the non-surviving (dead) root tissues. Co-occurrence patterns and network centrality analyses enabled us to discern the intricate characteristics of operational taxonomic units (OTUs) within endophytic and rhizospheric networks. Additionally, we isolated and identified bacterial strains that enhanced soybean tolerance to drought stresses, which were sourced from soybean plants under a drought field condition. The 16S rDNA sequence analysis revealed that the beneficial bacterial strains belong to the genera <i>Acinetobacter</i>, <i>Pseudomonas</i>, <i>Enterobacter</i>, and <i>Stenotrophomonas</i>. Specific bacterial strains, particularly those identified as <i>Acinetobacter pittii</i> and <i>Pseudomonas</i> sp., significantly enhanced plant growth metrics and reduced drought stress indices in soybean plants through seed treatment. Overall, this study advances our understanding of the soybean-associated microbiome structure under drought stress, paving the way for future research to develop innovative strategies and biological tools for enhancing soybean resilience to drought.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Polyphosphate as an Inorganic Chaperone to Prevent Protein Aggregation Under Copper Stress in <i>Saccharolobus solfataricus</i>.","authors":"José Acevedo-López, Gabriela González-Madrid, Claudio A Navarro, Carlos A Jerez","doi":"10.3390/microorganisms12122627","DOIUrl":"https://doi.org/10.3390/microorganisms12122627","url":null,"abstract":"<p><p>Polyphosphates are biopolymers composed of phosphate monomers linked by high-energy phosphoanhydride bonds. They are present across all life domains, serving as a source of energy, metal chelators, and playing a crucial role in stress defense. In <i>Escherichia coli</i>, polyphosphates also function as inorganic molecular chaperones. The present study aims to investigate whether polyphosphate serves a similar chaperone function in archaea, using <i>Saccharolobus solfataricus</i> as a model organism. To this end, polyphosphate was extracted and quantified, the ADP/ATP ratio was determined, insoluble protein extracts were analyzed at different time points after copper exposure, and qPCR was performed to measure the expression of stress-related genes. PolyP was extracted after exposing the archaeon <i>S. solfataricus</i> to different copper concentrations. We determined that polyP degradation is directly correlated with metal concentration. At the minimum inhibitory concentration (MIC) of 2 mM Cu²⁺, polyP degradation stabilized 2 h after exposure and showed no recovery even after 24 h. The ADP/ATP ratio was measured and showed differences in the presence or absence of polyP. The analysis of proteins precipitated under copper stress showed a higher proportion of insoluble proteins at an elevated metal concentration. On the other hand, increased protein precipitation was detected in the absence of polyP. Gene expression analysis via qPCR was conducted to assess the expression of genes involved in chaperone and chaperonin production, copper resistance, oxidative stress response, and phosphate metabolism under prolonged copper exposure, both in the presence and absence of polyP. The results indicated an upregulation of all the chaperonins measured in the presence of polyP. Interestingly, just some of these genes were upregulated in polyP's absence. Despite copper stress, there was no upregulation of superoxide dismutase in our conditions. These results highlight the role of polyP in the copper stress response in <i>S. solfataricus</i>, particularly to prevent protein precipitation, likely due to its function as an inorganic chaperone. Additionally, the observed protein precipitation could be attributable to interactions between copper and some amino acids on the protein structures rather than oxidative stress induced by copper exposure, as previously described in <i>E. coli</i>. Our present findings provide new insights into the protective role of polyP as an inorganic chaperone in <i>S. solfataricus</i> and emphasize its importance in maintaining cellular homeostasis under metal stress conditions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Diversity and Virulence Factors of <i>Clostridium perfringens</i> Isolated from Healthy and Necrotic Enteritis-Affected Broiler Chicken Farms in Quebec Province.","authors":"Sara Heidarpanah, Kevin Li, Alexandre Thibodeau, Ilhem Meniaï, Valeria R Parreira, Sylvain Quessy, Mariela Segura, Nahuel Fittipaldi, Marie-Lou Gaucher","doi":"10.3390/microorganisms12122624","DOIUrl":"https://doi.org/10.3390/microorganisms12122624","url":null,"abstract":"<p><p>Avian necrotic enteritis due to the Gram-positive bacterium <i>Clostridium perfringens</i> has re-emerged following the ban on antibiotic growth promoters in many poultry producing countries. The limited number of previous studies has left important gaps in our understanding of the genetic diversity and virulence traits of the pathogen. To address these knowledge gaps, in this study, we sequenced the genomes of 41 <i>Clostridium perfringens</i> isolates recovered from commercial broiler chicken flocks in Quebec, Canada, including isolates from healthy birds and those affected by necrotic enteritis. We sought to understand the pangenome diversity and interrogated the genomes for key virulence factors involved in necrotic enteritis pathogenesis. On average, the genomes had a GC content of 28% and contained 3206 coding sequences. A variable presence of toxins, degradative hydrolytic enzymes, and collagen-binding proteins was also found. Through pangenome analysis, we revealed a total of 10,223 genes, 652 (6.4%) of which formed the core genome. Additionally, we identified 17 different plasmids, 12 antibiotic resistance genes, and nine prophage regions. Overall, our results demonstrated a relatively high genetic diversity among chicken <i>Clostridium perfringens</i> isolates collected from the same geographical location, offering new insights into potential virulence mechanisms and adaptation of the pathogen within poultry populations.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain).","authors":"Nereida M Rancel-Rodríguez, Nicole Sausen, Carolina P Reyes, Antera Martel Quintana, Barbara Melkonian, Michael Melkonian","doi":"10.3390/microorganisms12122625","DOIUrl":"https://doi.org/10.3390/microorganisms12122625","url":null,"abstract":"<p><p>A total of 96 strains of Nostocales (Cyanobacteria) were established from the phyllosphere of the laurel forests in the Canary Islands (Spain) and the Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized by light microscopy and SSU rRNA gene comparisons. Morphologically, most strains belonged to two different morphotypes, termed \"<i>Nostoc</i>-type\" and \"<i>Tolypothrix</i>-type\". Molecular phylogenetic analysis of 527 SSU rRNA gene sequences of cyanobacteria (95 sequences established during this study plus 392 sequences from Nostocales and 40 sequences from non-heterocyte-forming cyanobacteria retrieved from the databases) revealed that none of the SSU rRNA gene sequences from the phyllosphere of the laurel forests was identical to a database sequence. In addition, the genetic diversity of the isolated strains was high, with 42 different genotypes (44% of the sequences) recognized. Among the new genotypes were also terrestrial members of the genus <i>Nodularia</i> as well as members of the genus <i>Brasilonema</i>. It is concluded that heterocyte-forming cyanobacteria represent a component of the phyllosphere that is still largely undersampled in subtropical/tropical forests.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations in Gut Microbiota Composition Are Associated with Changes in Emotional Distress in Children with Obstructive Sleep Apnea.","authors":"Chung-Guei Huang, Wan-Ni Lin, Li-Jen Hsin, Yu-Shu Huang, Li-Pang Chuang, Tuan-Jen Fang, Hsueh-Yu Li, Terry B J Kuo, Cheryl C H Yang, Chin-Chia Lee, Li-Ang Lee","doi":"10.3390/microorganisms12122626","DOIUrl":"https://doi.org/10.3390/microorganisms12122626","url":null,"abstract":"<p><p>Emerging evidence underscores the pivotal role of the gut microbiota in regulating emotional and behavioral responses via the microbiota-gut-brain axis. This study explores associations between pediatric obstructive sleep apnea (OSA), emotional distress (ED), and gut microbiome alterations before and after OSA treatment. Sixty-six children diagnosed with OSA via polysomnography participated, undergoing adenotonsillectomy alongside routine educational sessions. ED was assessed using the OSA-18 questionnaire, categorizing participants into high ED (scores ≥ 11, 52%) and low ED (scores < 11, 48%) groups. Gut microbiome analysis revealed significant diversity differences, with high ED linked to a reduced Shannon index (<i>p</i> = 0.03) and increased beta diversity (<i>p</i> = 0.01). Three months post-treatment, significant improvements were observed in OSA symptoms, ED scores, and gut microbiome alpha diversity metrics among 55 participants (all <i>p</i> < 0.04). Moreover, changes in the relative abundances of <i>Veillonella</i>, <i>Bifidobacterium</i>, <i>Flavonifractor</i>, and <i>Agathobacter</i>, as well as ultra-low frequency power and low frequency power of sleep heart rate variability, were independently associated with ED score alterations. These findings underscore the gut microbiome's critical role in the emotional and behavioral symptoms associated with pediatric OSA, suggesting that microbiome-targeted interventions could complement traditional treatments for ED reduction and emphasizing the need for further research.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Genes and Clonal Relationships of Duck-Derived <i>Salmonella</i> in Shandong Province, China in 2023.","authors":"Zhiyuan Lu, Yue Zheng, Shaopeng Wu, Xiaoyue Lin, Huiling Ma, Xiaofei Xu, Shumin Chen, Jiaqi Huang, Zheng Gao, Guisheng Wang, Shuhong Sun","doi":"10.3390/microorganisms12122619","DOIUrl":"https://doi.org/10.3390/microorganisms12122619","url":null,"abstract":"<p><p><i>Salmonella</i> is a major threat to both human and animal health. However, the diversity and antibiotic resistance of animal-derived <i>Salmonella</i> and their association with human infections remain largely unexplored. In this study, <i>Salmonella</i> strains were isolated, identified, and sequenced from dead embryos and cloacal swab samples obtained from 278 large-scale duck farms in 11 cities in Shandong Province. The results show that a total of 57 <i>Salmonella</i> strains were isolated, with the dominant sequence types (ST) being ST17 (15/57) and ST19 (9/57), while the dominant serotypes were <i>S. Indiana</i> (15/57) and <i>S. Typhimurium</i> (11/57). Furthermore, genomic analysis has revealed the presence of prevalent antibiotic resistance genes (ARGs), which are often associated with co-transfer mechanisms. Over 52.63% of the strains were observed to carry two or more ARGs, especially one <i>Salmonella</i> strain that carried twenty-eight distinct ARGs. Furthermore, core genome multilocus sequence typing analysis (cgMLST) indicated that the 57 <i>Salmonella</i> strains may have a close relationship, which could be clonally transmitted among different cities. The results demonstrated a close relationship between the <i>Salmonella</i> strains identified in diverse geographical regions, suggesting that these strains may have been widely disseminated through clonal transmission. The mutation analysis reveals significant mutations at <i>parC</i> (T57S), <i>gyrA</i> (S83F), <i>parC</i> (S80R), <i>gyrA</i> (D87N), and <i>gyrA</i> (S83Y). These findings emphasize the necessity for monitoring and controlling <i>Salmonella</i> infections in animals, as they may serve as a reservoir for ARGs with the potential to affect human health or even be the source of pathogens that infect humans.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pasteurella multocida</i> Serotype D Infection Induces Activation of the IL-17 Signaling Pathway in Goat Lymphocytes.","authors":"Yujing Fu, Yong Meng, Hejie Qian, Taoyu Chen, Xiangying Chen, Qiaoling Chen, Hongyan Gao, Churiga Man, Li Du, Si Chen, Fengyang Wang","doi":"10.3390/microorganisms12122618","DOIUrl":"https://doi.org/10.3390/microorganisms12122618","url":null,"abstract":"<p><p>(1) Background: Pasteurellosis is a global zoonotic bacterial disease, which has caused significant economic impacts in animal husbandry. Nevertheless, there is limited understanding of the immune response between goat peripheral blood lymphocytes (PBLs) and goat-derived <i>Pasteurella multocida</i> (<i>P. multocida</i>). (2) Methods: To investigate the immune response of host PBLs during infection with <i>P. multocida</i> type D, we established an <i>in vitro</i> cell model utilizing isolated primary goat PBLs. Utilizing this <i>in vitro</i> infection model, we employed an enzyme-linked immunosorbent assay (ELISA) to assess the cytokine profile variation in goat PBLs following infection. Meanwhile, RNA sequencing and quantitative PCR (qPCR) methods were employed to analyze the gene expression profile. (3) Results: The ELISA test results indicated that the expression levels of pro-inflammatory cytokines, such as IL-6, IFN-γ, CXCL10, and IL-17A, were significantly elevated within 12 h after infection with <i>P. multocida</i>. In contrast, the levels of the anti-inflammatory cytokine IL-10 were found to be reduced. RNA sequencing and functional enrichment analysis identified 2114 differentially expressed genes (DEGs) that were primarily associated with cytokine-cytokine receptor interactions, viral protein-cytokine interactions, and the IL-17 signaling pathway. Furthermore, protein-protein interaction (PPI) network analysis and qPCR highlighted <i>CD86</i>, <i>CCL5</i>, <i>CD8A</i>, <i>CXCL8</i>, <i>CTLA4</i>, <i>TNF</i>, <i>CD274</i>, <i>IL-10</i>, <i>IL-6</i>, <i>CXCL10</i>, <i>IFNG,</i> and <i>IL-17A</i> that were crucial for the response of PBLs to <i>P. multocida</i> infection. (4) Conclusions: This study systematically revealed the characteristics of PBLs in goats following infection with goat-derived <i>P. multocida</i> type D through the analysis of cytokines and gene expression, providing important theoretical insights for a deeper understanding of the defense mechanisms in goats against <i>P. multocida</i>.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}