Microorganisms最新文献

{"title":"Regulatory Mechanisms of Exogenous Acyl-Homoserine Lactones in the Aerobic Ammonia Oxidation Process Under Stress Conditions.","authors":"Chen Qiu, Kailing Pan, Yuxuan Wei, Xiaolin Zhou, Qingxian Su, Xuejun Bi, Howyong Ng","doi":"10.3390/microorganisms13030663","DOIUrl":"10.3390/microorganisms13030663","url":null,"abstract":"<p><p>This study investigated the mechanism by which N-acyl-homoserine lactone (AHL) signaling molecules influence ammonia-oxidizing microorganisms (AOMs) under inhibitory conditions. In laboratory-scale sequential batch reactors (SBRs), the effects of different AHLs (C6-HSL and C8-HSL) on the metabolic activity, microbial community structure, and quorum sensing (QS) system response of AOMs were examined. Caffeic acid, 1-octyne, and allylthiourea were used as ammoxidation inhibitors. The results indicated that under inhibitory conditions, AHLs effectively reduced the loss of ammonia oxidation activity and enhanced the resistance of AOMs to unfavorable environments. Additionally, AHLs enriched AOMs in the microbial community, wherein C6-HSL significantly increased the abundance of <i>amoA</i> genes in AOMs. Furthermore, AHLs maintained the activity of QS-related genes and preserved the communication ability between microorganisms. Correlation analysis revealed a positive relationship between AOMs and QS functional bacteria, suggesting that AHLs can effectively regulate the ammonia oxidation process. Overall, exogenous AHLs can improve the metabolic activity and competitive survival of AOMs under inhibitory conditions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Soil Physicochemical Properties and Inter-Root Microbial Communities on the Inhibition of Anthracnose in Peppers.","authors":"Yongbin Ma, Miaomiao Liu, Yuting Hong, Yichao Wang, Xiaoke Chang, Gongyao Shi, Huaijuan Xiao, Qiuju Yao, Fan Yang","doi":"10.3390/microorganisms13030661","DOIUrl":"10.3390/microorganisms13030661","url":null,"abstract":"<p><p>Anthracnose is a widespread plant disease affecting vegetables, flowers, crops, and fruit trees, causing significant economic losses. It occurs at various stages of pepper growth, leading to rotting and shedding in later stages. The aim of this study was to explore the relationship with anthracnose occurrence by analyzing the physicochemical properties and microbiota changes in the inter-root soil of pepper under different susceptibility levels to reveal the key microecological factors and dominant microbial populations and to provide reference for ecological control. Illumina Miseq sequencing was first used to evaluate the physicochemical properties and microbial taxa in pepper inter-root soil across different health statuses and identify key parameters associated with anthracnose. Subsequently, PICRUSt2 (systematic genetic Investigation of communities by Reconstruction of observed States 2) and FUNGuild (Fungi Functional Guild) V1.0 online platform were used to predict the activities of inter-root bacteria and fungi. The findings indicated that healthy peppers had significantly higher inter-root soil nutrient levels and enzyme activity compared to sensitive peppers. There were significant differences between their community structures. In alpha-diversity analysis, inter-root soil microbial richness and diversity were significantly higher in healthy peppers than in susceptible peppers. At the bacterial taxonomic level, the comparative prevalence of Acidobacteria in highly resistant plants, resistant plants, and susceptible plants decreased sequentially. At the genus level, the relative abundance of <i>Vicinamibacteraceae</i> and <i>RB41</i> was markedly elevated in disease-resistant inter-root soils than in disease-susceptible soils. At the fungal level, the comparative prevalence of <i>Ascomycetes</i> in highly resistant plants, resistant plants, and susceptible plants increased sequentially. Differences in function are mainly manifested in apoptosis and mycelial development.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Insights into Selenate Reduction by <i>Anaerobacillus</i> Species.","authors":"Qidong Wang, Jian Zhang, Jinhui Liang, Yanlong Wang, Chongyang Ren, Xinhan Chen, Dongle Cheng, Huanxin Zhang, Huaqing Liu","doi":"10.3390/microorganisms13030659","DOIUrl":"10.3390/microorganisms13030659","url":null,"abstract":"<p><p>Selenium (Se), a potentially toxic trace element, undergoes complex biogeochemical cycling in the environment, largely driven by microbial activity. The reduction in selenate or selenite to elemental selenium is an environmentally beneficial process, as it decreases both Se toxicity and mobility. This reduction is catalyzed by enzymes encoded by various related genes. The link between Se reduction gene clusters and specific taxonomic groups is significant for elucidating the ecological roles and processes of Se reduction in diverse environments. In this study, a new species of Se-reducing microorganism belonging to the genus <i>Anaerobacillus</i> was isolated from a mining site. A comparative analysis of the growth characteristics reveals that <i>Anaerobacillus</i> species exhibit notable metabolic versatility, particularly in their fermentation abilities and utilization of diverse electron donors and acceptors. Genome analysis identified a diverse array of gene clusters associated with selenate uptake (<i>sul</i>, <i>pst</i>), selenate reduction (<i>ser</i>), and selenite reduction (<i>hig</i>, <i>frd</i>, <i>trx</i>, and <i>bsh</i>). Since selenate reduction is the first crucial step in Se reduction, genes linked to selenate reductase are the focus. The <i>serA</i> gene clusters analysis suggests that the <i>serA</i> gene is highly conserved across <i>Anaerobacillus</i> species. The surrounding genes of <i>serA</i> show significant variability in both presence and gene size. This evolutionary difference in coenzyme utilization and <i>serA</i> regulation suggests distinct survival strategies among <i>Anaerobacillus</i> species. This study offers insights into Se bio-transformations and the adaptive strategies of Se-reducing microorganisms.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Glyphosate Resistance and Degradation Profile of <i>Caballeronia zhejiangensis</i> CEIB S4-3 and Genes Involved in Its Degradation.","authors":"Manuel Isaac Morales-Olivares, María Luisa Castrejón-Godínez, Patricia Mussali-Galante, Efraín Tovar-Sánchez, Hugo Albeiro Saldarriaga-Noreña, Alexis Rodríguez","doi":"10.3390/microorganisms13030651","DOIUrl":"10.3390/microorganisms13030651","url":null,"abstract":"<p><p>Herbicides are the most employed pesticides in agriculture worldwide; among them, glyphosate is the most successful herbicide molecule in history. The extensive use of glyphosate has been related to environmental pollution and toxic effects on non-target organisms. Effective remediation and treatment alternatives must be developed to reduce the environmental presence of glyphosate and its adverse effects. Bioremediation using microorganisms has been proposed as a feasible alternative for treating glyphosate pollution; due to this, identifying and characterizing microorganisms capable of biodegrading glyphosate is a key environmental task for the bioremediation of polluted sites by this herbicide. This study characterized the glyphosate resistance profile and degradation capacity of the bacterial strain <i>Caballeronia zhejiangensis</i> CEIB S4-3. According to the results of the bacterial growth inhibition assays on agar plates, <i>C. zhejiangensis</i> CEIB S4-3 can resist exposure to high concentrations of glyphosate, up to 1600 mg/L in glyphosate-based herbicide (GBH) formulation, and 12,000 mg/L of the analytical-grade molecule. In the inhibition assay in liquid media, <i>C. zhejiangensis</i> CEIB S4-3 resisted glyphosate exposure to all concentrations evaluated (25-400 mg/L). After 48 h exposure, GBH caused important bacterial growth inhibition (>80%) at concentrations between 100 and 400 mg/L, while exposure to analytical-grade glyphosate caused bacterial growth inhibitions below 15% in all tested concentrations. Finally, this bacterial strain was capable of degrading 60% of the glyphosate supplemented to culture media (50 mg/L), when used as the sole carbon source, in twelve hours; moreover, <i>C. zhejiangensis</i> CEIB S4-3 can also degrade the primary glyphosate degradation metabolite aminomethylphosphonic acid (AMPA). Genomic analysis revealed the presence of genes associated with the two reported metabolic pathways for glyphosate degradation, the sarcosine and AMPA pathways. This is the first report on the glyphosate degradation capacity and the genes related to its metabolism in a <i>Caballeronia</i> genus strain. The results from this investigation demonstrate that <i>C. zhejiangensis</i> CEIB S4-3 exhibits significant potential for glyphosate biodegradation, suggesting its applicability in bioremediation strategies targeting this contaminant.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Diversity in Pet Rabbits: Implications for Public Health, Zoonotic Risks, and Antimicrobial Resistance.","authors":"Smaranda Crăciun, Cristiana Ştefania Novac, Nicodim Iosif Fiţ, Cosmina Maria Bouari, Lucia Victoria Bel, George Cosmin Nadăş","doi":"10.3390/microorganisms13030653","DOIUrl":"10.3390/microorganisms13030653","url":null,"abstract":"<p><p>This study examined epidemiological aspects of rabbit pathologies, identified bacterial strains, and assessed their antimicrobial resistance, emphasizing rabbits as potential reservoirs for zoonotic multidrug resistant (MDR) bacteria and the need for continuous monitoring and antimicrobial stewardship. Samples from rabbits were cultivated and then identified using Vitek^® 2 and MALDI-TOF. Antimicrobial susceptibility was assessed by disk diffusion testing. This study analyzed 170 individuals with various pathologies, with males (58.24%) outnumbering females (41.76%). Dental abscesses (35.29%) and respiratory infections (28.24%) were most common. Antibiotic exposure was noted in 47.06% of cases, primarily involving trimethoprim (35.56%). Of the total samples, 91.18% tested positive, revealing 200 isolates from 23 bacterial genera, with <i>Staphylococcus</i> spp. (31%) and <i>Escherichia coli</i> (12%) being most frequently identified as well as species with zoonotic potential, such as <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, and <i>Enterococcus faecium</i>. Antimicrobial susceptibility testing showed high efficacy for florfenicol (75%), ciprofloxacin (74.12%), and amikacin (68.65%), while significant resistance was found for kanamycin, neomycin, and trimethoprim. Nearly 49% of strains were MDR, with Gram-positive cocci, Enterobacteriaceae, and non-Enterobacteriaceae showing varying resistance, across 18 MDR genera. In conclusion, pet rabbits are potential reservoirs of zoonotic and MDR bacterial species, posing a risk for their owners.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of Potato Dextrose Agar Culture Medium for Actinobacteria Growth.","authors":"Elian Chaves Ribeiro, Emanuelle Ketthlen Nunes Araújo, Margareth Santos Costa Penha, Adriana Silva do Nascimento, Darlan Ferreira da Silva, Rita de Cássia Mendonça de Miranda","doi":"10.3390/microorganisms13030654","DOIUrl":"10.3390/microorganisms13030654","url":null,"abstract":"<p><p>The objective of this study was to optimise the potato dextrose agar (PDA) culture medium in terms of its potential for use in the growth of actinobacteria. The strain used in this study was a species of actinobacteria previously identified as <i>Streptosporangium</i> sp. (P1C3), characterised by slow growth (20 days of incubation), low aerial mycelium production, and no pigment production. To determine the optimal formulation, the <i>Streptosporangium</i> sp. (P1C3) strain was tested for incubation time and aerial mycelium growth across 27 formulations based on the PDA culture medium. A central composite rotational design (CCRD) experimental methodology was employed, where glucose concentration (g/L), yeast extract concentration (g/L), pH, and temperature were tested. Among the tested formulations, 01, 05, 09, and 13 showed a reduction in incubation time and complete aerial mycelium growth, which was linearly influenced by the four tested variables. Response surface analysis indicated that the optimal values for promoting aerial mycelium growth in the shortest incubation time were 10 g/L glucose concentration, from 1 g/L to 3 g/L yeast extract concentration, pH levels between 5.7 and 7.2, and temperatures between 24 °C and 32 °C. The optimisation of the PDA medium proved effective in improving the isolation of actinobacteria and enhancing the production of metabolites with potential antimicrobial activity.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candida auris</i>: A Continuing Threat.","authors":"Ashish Bhargava, Katherine Klamer, Mamta Sharma, Daniel Ortiz, Louis Saravolatz","doi":"10.3390/microorganisms13030652","DOIUrl":"10.3390/microorganisms13030652","url":null,"abstract":"<p><p><i>Candida auris</i> is a World Health Organization critical-priority fungal pathogen that has variable resistance to antifungal treatments. Multiple clades have been identified through genomic analysis and have appeared in different geographic locations simultaneously. Due to a combination of factors including antifungal resistance, ability to colonize and persist in the environment, and thermotolerance, it can thrive. Infected patients are associated with a high mortality rate, especially those with multiple health risk factors like those associated with other <i>Candida</i> species. This review highlights the current situation of this pathogen to help provide guidance for future work.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outpatient Testing for HIV in Italy, 2018-2023-Preliminary Data.","authors":"Claudio Galli, Vincenza Regine, Anna Caraglia, Francesca Centrone, Maria Chironna, Gianluca Cruschelli, Massimo Farinella, Valentina Annachiara Orlando, Chiara Pasqualini, Monia Puglia, Lucia Pugliese, Laura Rancilio, Lara Tavoschi, Fabio Voller, Barbara Suligoi","doi":"10.3390/microorganisms13030655","DOIUrl":"10.3390/microorganisms13030655","url":null,"abstract":"<p><p>HIV testing is crucial towards the control of the Acquired Immune Deficiency Syndrome (AIDS) epidemic. Monitoring trends of human immunodeficiency virus (HIV) testing over time may help interpret the incidence of new HIV diagnoses and effectiveness of HIV testing strategies. We started a research project aimed at assessing testing rates for HIV infection among Italian outpatients in 2018-2023. Numeric data for screening, confirmatory, and monitoring tests obtained by a national register were compared with the numbers of adult residents, newly diagnosed HIV infections, and patients undergoing treatment. The number of screening tests declined from 1,133,377 in 2018 to 889,972 in 2020 and increased to 1,096,822 in 2023. HIV-RNA tests showed a similar pattern, whereas confirmatory immunoblots did not vary significantly over time. The ratio of screening tests to adult residents was higher in North-West (2.87%) and North-East (2.31%) Italy compared to South Italy and the islands (1.47%), indicating that screening should be enhanced in the latter area. We observed differences between the ratio of screening tests and the incidence of newly diagnosed HIV infections by geographic area. Discrepancies in the number of screening and confirmatory tests needed for each new diagnosis suggest repeated testing on people already diagnosed and possible data reporting issues. The monitoring of HIV screening tests at the national and regional levels can provide essential data to interpret trends in HIV epidemiology and plan relevant testing strategies over time.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process Technologies for Disinfection of Food-Contact Surfaces in the Dry Food Industry: A Review.","authors":"Harleen Kaur Dhaliwal, Shivani Sonkar, Prithviraj V, Luis Puente, M S Roopesh","doi":"10.3390/microorganisms13030648","DOIUrl":"10.3390/microorganisms13030648","url":null,"abstract":"<p><p>The survival characteristics of bacterial pathogens, including <i>Salmonella</i> spp., <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus,</i> and <i>Escherichia coli,</i> in foods with a low water activity (<i>a_w</i>) have been extensively examined and reported. Microbial attachment on the food-contact surfaces can result in cross-contamination and compromise the safety of low-<i>a_w</i> foods. The bactericidal potential of various conventional and novel disinfection technologies has been explored in the dry food industry. However, the attachment behavior of bacterial pathogens to food-contact surfaces in low-<i>a_w</i> conditions and their subsequent response to the cleaning and disinfection practices requires further elucidation. The review summarizes the elements that influence disinfection, such as the presence of organic residues, persistent strains, and the possibility of microbial biotransfer. This review explores in detail the selected dry disinfection technologies, including superheated steam, fumigation, alcohol-based disinfectants, UV radiation, and cold plasma, that can be used in the dry food industry. The review also highlights the use of several wet disinfection technologies employing chemical antimicrobial agents against surface-dried microorganisms on food-contact surfaces. In addition, the disinfection efficacy of conventional and novel technologies against surface-dried microorganisms on food-contact surfaces, as well as their advantages and disadvantages and underlying mechanisms, are discussed. Dry food processing facilities should implement stringent disinfection procedures to ensure food safety. Environmental monitoring procedures and management techniques are essential to prevent adhesion and allow the subsequent inactivation of microorganisms.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"(Re)Emerging Arboviruses of Public Health Significance in the Brazilian Amazon.","authors":"Kyndall C Dye-Braumuller, Rebecca A Prisco, Melissa S Nolan","doi":"10.3390/microorganisms13030650","DOIUrl":"10.3390/microorganisms13030650","url":null,"abstract":"<p><p>Brazil is one of the most important countries globally in regard to arboviral disease ecology and emergence or resurgence. Unfortunately, it has shouldered a majority of arboviral disease cases from Latin America and its rich flora, fauna (including arthropod vectors), and climate have contributed to the vast expansion of multiple arboviral diseases within its borders and those that have expanded geographically outside its borders. Anthropogenic landscape changes or human-mediated changes such as agriculture, deforestation, urbanization, etc. have all been at play within the country in various locations and can also be attributed to arboviral movement and resurgence. This review describes a brief history of landscape changes within the country and compiles all the known information on all arboviruses found within Brazil (endemic and imported) that are associated with human disease and mosquitoes including their original isolation, associated vertebrate animals, associated mosquitoes and other arthropods, and human disease symptomology presentations. This information is crucial as the Western Hemisphere is currently experiencing multiple arbovirus outbreaks, including one that originated in the Brazilian Amazon. Understanding which arboviruses are and have been circulating within the country will be pertinent as anthropogenic landscape changes are consistently being perpetrated throughout the country, and the occurrence of the next arbovirus epidemic will be a matter of when, not if.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}