Microorganisms最新文献

{"title":"Geosmin Events Associated with <i>Dolichospermum circinale</i> Abundance Promoted by Nitrogen Supply in a Chinese Large Tropical Eutrophic Reservoir.","authors":"Li-Juan Xiao, Yanru Jiang, Zihan Chen, Liang Peng, Yali Tang, Lamei Lei","doi":"10.3390/microorganisms12122610","DOIUrl":"https://doi.org/10.3390/microorganisms12122610","url":null,"abstract":"<p><p>Taste and odor (T/O) compounds are a global threat in drinking water, mainly produced by cyanobacteria in freshwater environments. Temperature plays a crucial role in regulating geosmin dynamics in temperate and subtropical lakes, while its influence may be lower in tropical waters. To better understand the factors affecting geosmin occurrence in tropical waters, a dataset from a field investigation conducted in a large tropical reservoir was analyzed. The water temperature varied between 16 °C and 32 °C, with geosmin concentration ranging from below the detection limit (3 ng/L) to as high as 856 ng/L. Elevated geosmin levels exceeding > 10 ng/L were observed over the whole year except for in September, suggesting that the annual temperature was suitable for geosmin production. Among the diverse cyanobacteria, <i>Dolichospermum circinale</i> was identified as the main producer of geosmin in the reservoir, both by correlation analysis and cells' geosmin measurements. Geosmin concentration was also significantly related to the abundance of <i>D. circinale.</i> None of the environmental variables (temperature, pH, transparency and nutrients) were significantly directly correlated with geosmin concentration. But the high total nitrogen significantly explained the increase in <i>D. circinale</i> abundance associated with geosmin elevation. Our results suggest that nutrients, particularly nitrogen, directly affected the competitive advantage and abundance of key geosmin producers and thus modified geosmin levels in this tropical reservoir. Our study thus hints at the possible management of the geosmin problem through nutrient reduction in tropical reservoirs.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoarchitectonics for Advancing Bone Graft Technology: Integration of Silver Nanoparticles Against Bacteria and Fungi.","authors":"Leticia Ramos Dantas, Maria Alice Witt, Everdan Carneiro, Felipe Francisco Tuon","doi":"10.3390/microorganisms12122616","DOIUrl":"https://doi.org/10.3390/microorganisms12122616","url":null,"abstract":"<p><p>Silver nanoparticles have garnered significant attention for their antimicrobial applications. The aim of this study was to develop and characterize a silver nanoparticle-enhanced bone graft and assess its antimicrobial and antibiofilm activities. Bone granules from bovine cancellous femur were impregnated with silver nanoparticles (50 nm). The antimicrobial and antibiofilm activity was tested against various pathogens, including Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis, Acinetobacter baumannii, and Escherichia coli. Biocompatibility and resorption were evaluated in a mouse calvaria model. All the tested pathogens showed susceptibility to silver nanoparticles, with minimal inhibitory concentrations ranging from 0.25 to 4 mg/L. The silver nanoparticle scaffolds demonstrated a significant reduction in biofilm formation across all microorganisms. The graft exhibited a biocompatibility comparable to that of autologous bone, with reduced resorption rates. Additionally, the presence of nanoparticles did not impact radiolucency, and cytotoxicity remained minimal. Bone grafts impregnated with silver nanoparticles effectively reduce biofilm formation, suggesting their potential as a strategic material for various implant applications.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Climate and Edaphic Factors on the Community Composition of Biocrusts Along an Elevation Gradient in the High Arctic.","authors":"Isabel Mas Martinez, Ekaterina Pushkareva, Leonie Agnes Keilholz, Karl-Heinz Linne von Berg, Ulf Karsten, Sandra Kammann, Burkhard Becker","doi":"10.3390/microorganisms12122606","DOIUrl":"https://doi.org/10.3390/microorganisms12122606","url":null,"abstract":"<p><p>Biological soil crusts are integral to Arctic ecosystems, playing a crucial role in primary production, nitrogen fixation and nutrient cycling, as well as maintaining soil stability. However, the composition and complex relationships between the diverse organisms within these biocrusts are not well studied. This study investigates how the microbial community composition within Arctic biocrusts is influenced by environmental factors along an altitudinal gradient (101 m to 314 m). Metagenomic analyses were used to provide insights into the community composition, revealing that temperature, pH, and nutrient availability significantly shaped the community. In contrast, altitude did not directly influence the microbial composition significantly. Eukaryotic communities were dominated by Chloroplastida and fungi, while Proteobacteria and Actinobacteria prevailed among prokaryotes. Cyanobacteria, particularly orders such as Pseudoanabaenales, Pleurocapsales, and Nostocales, emerged as the most abundant photoautotrophic organisms. Our findings highlight the impact of environmental gradients on microbial diversity and the functional dynamics of biocrusts, emphasizing their critical role in Arctic tundra ecosystems. Arctic biocrusts are intricate micro-ecosystems, whose structure is strongly shaped by local physicochemical parameters, likely affecting essential ecological functions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Recombinant Clostridial Neurotoxin by <i>C. tetani</i>.","authors":"Brieana M Gregg, Sonal Gupta, William H Tepp, Sabine Pellett","doi":"10.3390/microorganisms12122611","DOIUrl":"https://doi.org/10.3390/microorganisms12122611","url":null,"abstract":"<p><p>Tetanus neurotoxins (TeNT) and botulinum neurotoxins (BoNTs) are closely related ~150 kDa protein toxins that together comprise the group of clostridial neurotoxins (CNTs) expressed by various species of <i>Clostridia</i>. While TeNT is expressed as a single polypeptide, BoNTs are always produced alongside multiple non-toxic proteins that form a stabilizing complex with BoNT and are encoded in a conserved toxin gene cluster. It is unknown how <i>tent</i> evolved without a similar gene cluster and why complex-free TeNT is secreted as a stable and soluble protein by <i>C. tetani</i>, whereas complexing proteins appear to be essential for BoNT stability in culture supernatants of <i>C. botulinum</i>. To assess whether the stability of TeNT is due to an innate property of the toxin or is a result of <i>C. tetani</i>'s intra- and extra-cellular environment, both TeNT and complex-free BoNT/A1^ERY were expressed recombinantly in atoxic <i>C. tetani</i> and analyzed for expression and stability. The strong clostridial ferredoxin (<i>fdx</i>) promotor resulted in the expression of recombinant TeNT at greater levels and earlier time points than endogenously produced TeNT. Recombinant BoNT/A1^ERY was similarly expressed by atoxic <i>C. tetani</i>, although partial degradation was observed. The rBoNT/A1^ERY produced in <i>C. tetani</i> was also partially proteolytically processed to the dichain form. Investigations of bacterial growth media and pH conditions found that the stability of rTeNT and rBoNT/A1^ERY in spent media of <i>C. tetani</i> or <i>C. botulinum</i> was affected by growth media but not by pH. These data indicate that the distinct metabolism of <i>C. tetani</i> or <i>C. botulinum</i> under various growth conditions is a primary factor in creating a more or less favorable environment for complex-free CNT stability.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunnel Infection and Peritonitis Induced by <i>Staphylococcus aureus</i> Due to Decubitus Change of the Anterior Abdominal Wall in a Patient on Peritoneal Dialysis: Case Report.","authors":"Marko Baralić, Ana Bontić, Jelena Pavlović, Vidna Karadžić-Ristanović, Selena Gajić, Jovan Jevtić, Pavle Popović, Kristina Petrović, Lara Hadži-Tanović, Aleksandra Kezić","doi":"10.3390/microorganisms12122608","DOIUrl":"https://doi.org/10.3390/microorganisms12122608","url":null,"abstract":"<p><p>The occurrence of anterior abdominal wall ulcer at the site of the peritoneal catheter (PC) is one of the rarest complications of peritoneal dialysis (PD). When present, it is mainly caused by <i>staphylococci</i> which respond well to vancomycin therapy. Despite well-conducted therapy, there is a tendency to relapse and induce peritonitis, which makes it necessary to remove the PC and change the dialysis model of treatment and/or re-insert the catheter at another place to preserve PD as a treatment method. In the present study, we discuss a case of a 53-year-old patient with end-stage kidney disease treated with PD and with decubitus changes at the PC exit site; the change occurred due to migration of the catheter middle part by protruding from the abdominal cavity to the skin, thus allowing ulcer appearance. Although the PC site was treated with antibiotics, as advised by the surgeon, the patient was finally transferred to hemodialysis as the repositioning of the catheter was not performed. This leads to the conclusion that the antibiotic treatment and catheter repositioning are mandatory to preserve peritoneal dialysis as an end-stage kidney disease (ESKD) treatment model.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistant <i>Rhodococcus</i> for Biodegradation of Diesel Fuel at High Concentration and Low Temperature.","authors":"Irina Ivshina, Maria Kuyukina, Anastasiia Krivoruchko, Andrey Elkin, Tatyana Peshkur, Colin J Cunningham","doi":"10.3390/microorganisms12122605","DOIUrl":"https://doi.org/10.3390/microorganisms12122605","url":null,"abstract":"<p><p>The resistance of 16 <i>Rhodococcus</i> strains to diesel fuel was studied. The minimal inhibitory concentrations of diesel fuel against <i>Rhodococcus</i> were 4.0-64.0 vol. % and 0.5-16.0 vol. % after 7 days of incubation in Luria-Bertani broth and a mineral \"<i>Rhodococcus-</i>surfactant\" medium, respectively. The three most resistant strains (<i>R. ruber</i> IEGM 231, IEGM 442 and <i>Rhodococcus</i> sp. IEGM 1276) capable of overcoming the toxicity of diesel fuel at a high (8.0 vol. %) concentration and at a low (4 °C) temperature were selected. Respiration activities, growth kinetics, and changes in the diesel fuel composition during the biodegradation process were elucidated using gas chromatography with mass spectrometry, respirometry, and Bradford analysis. Growth conditions were optimised for the improved biodegradation of diesel fuel by <i>Rhodococcus</i> cells using multifactor analysis. They included the simultaneous addition of 1.3 g·L^-1 of granular sugar and 0.25 g·L^-1 of yeast extract. The twofold stimulation of the biodegradation of individual hydrocarbons in diesel fuel (<i>n-</i>pentadecane, <i>n-</i>hexadecane and <i>n-</i>heptadecane) was demonstrated when glycolipid <i>Rhodococcus-</i>biosurfactants were added at a concentration of 1.4 g·L^-1. A total removal of 71-91% of diesel fuel was achieved in this work.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Poultry Nutrition to Combat <i>Salmonella</i>: Insights from the Literature.","authors":"Muhammad Naeem, Dianna Bourassa","doi":"10.3390/microorganisms12122612","DOIUrl":"https://doi.org/10.3390/microorganisms12122612","url":null,"abstract":"<p><p><i>Salmonella</i> infections in poultry production are a significant and pervasive concern, posing substantial risks to both animal and human health. This comprehensive literature review examines the current body of research on the use of various nutritional manipulations as a promising strategy to effectively control and mitigate the prevalence of <i>Salmonella</i> in poultry. The review covers a range of dietary interventions, particularly the utilization of probiotics, prebiotics, organic acids, and phytochemicals, and thoroughly evaluates their efficacy in reducing <i>Salmonella</i> colonization within poultry flocks. Furthermore, the review delves into the potential underlying mechanisms of action for these nutritional approaches to control <i>Salmonella</i> and the far-reaching implications for overall food safety. By providing a thorough analysis of the existing literature, this review aims to elucidate the most promising nutritional strategies that can be employed to control <i>Salmonella</i> in poultry production, ultimately safeguarding animal welfare and public health.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating How Genomic Contexts Impact IS5 Transposition Within the <i>Escherichia coli</i> Genome.","authors":"Jonathan Onstead, Zhongge Zhang, Jialu Huo, Jack W Ord, Sofia Smith, Milton H Saier","doi":"10.3390/microorganisms12122600","DOIUrl":"https://doi.org/10.3390/microorganisms12122600","url":null,"abstract":"<p><p>Insertions of the transposable element IS5 into its target sites in response to stressful environmental conditions, DNA structures, and DNA-binding proteins are well studied, but how the genomic contexts near IS5's native loci impact its transpositions is largely unknown. Here, by examining the roles of all 11 copies of IS5 within the genome of <i>E. coli</i> strain BW25113 in transposition, we reveal that the most significant copy of IS5 is one nested within and oriented in the same direction as the <i>nmpC</i> gene, while two other copies of IS5 harboring point mutations are hardly transposed. Transposition activity is heavily reliant on the upstream <i>nmpC</i> promoter that drives IS5 transposase gene <i>ins5A</i>, with more transpositions resulting from greater promoter activity. The IS5 element at <i>nmpC</i> but not at other loci transcribed detectable amounts of <i>ins5A</i> mRNA. By increasing expression of the <i>ins5CB</i> operon harbored in IS5, we demonstrate that Ins5B and Ins5C appear to exert a stimulatory role in IS5 transposition, suggesting that the downstream genomic regions near the native loci are involved in overall IS5 transposition as well. Using a strain that carries IS5 only at the <i>nmpC</i> locus, we confirm that IS5 primarily uses a copy/paste mechanism for transposition, although we cannot rule out the cut/paste mechanism.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Betaherpesviruses in Neotropical Bats on the Caribbean Island of St. Kitts: First Report from Antillean Tree Bats (<i>Ardops nichollsi</i>) and Evidence for Cross-Species Transmission.","authors":"Jessica L Kulberg, Sarah Hooper, Yashpal S Malik, Souvik Ghosh","doi":"10.3390/microorganisms12122603","DOIUrl":"https://doi.org/10.3390/microorganisms12122603","url":null,"abstract":"<p><p>To date, limited information is available on herpesviruses in bats from the Caribbean region. We report here high detection rates (24.24%, n = 66) of herpesviruses in oral samples from apparently healthy bats (<i>Ardops nichollsi</i> (75%, 9/12) and <i>Molossus molossus</i> (28%, 7/25)) on the Lesser Antillean Island of St. Kitts. Based on analysis of partial DNA polymerase (DPOL) sequences (~225 amino acid (aa) residues), we identified two distinct groups of herpesviruses (BO-I and -II) that were unique to <i>A. nichollsi</i> and <i>M. molossus</i>, respectively. Within the subfamily <i>Betaherpesvirinae</i>, the BO-I DPOL sequences shared low deduced aa identities (<70%) with other herpesviruses, and phylogenetically, they formed a distinct cluster, representing a putative novel betaherpesvirus. The BO-II DPOL sequences were closely related to a putative novel betaherpesvirus from a <i>M. molossus</i> in Lesser Antillean Island of Martinique, indicating possible transmission of herpesviruses by bat movement between the Caribbean Islands. Phylogenetically, the BO-I and -II betaherpesviruses exhibited species-specific (<i>A. nichollsi</i> and <i>M. molossus</i>, respectively) as well as family-specific (<i>Phyllostomidae</i> and <i>Molossidae</i>, respectively) clustering patterns, corroborating the hypothesis on host specificity of betaherpesviruses. Interestingly, a single <i>M. molossus</i> betaherpesvirus strain clustered with the <i>A. nichollsi</i> betaherpesviruses, indicating possible interspecies transmission of herpesviruses between <i>Phyllostomidae</i> and <i>Molossidae</i>. To our knowledge, this is the first report on detection of herpesviruses from Antillean tree bats (<i>A. nichollsi</i>), expanding the host range of betaherpesviruses. Taken together, the present study identified putative novel betaherpesviruses that might be unique to chiropteran species (<i>A. nichollsi</i> and <i>M. molossus)</i>, indicating virus-host coevolution, and provided evidence for interspecies transmission of betaherpesviruses between chiropteran families.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discordant β-Lactam Susceptibility in Clinical <i>Staphylococcus aureus</i> Isolates: A Molecular and Phenotypical Exploration to Detect the BORSA/MODSA Isolates in Bogotá, Colombia.","authors":"Angie Lorena Fonseca-Fernández, María Alejandra Mancera-García, Aura Lucia Leal-Castro, Chad Leidy, Sandra Rincón, Lina P Carvajal, Jinnethe Reyes, Adriana Marcela Celis Ramírez","doi":"10.3390/microorganisms12122598","DOIUrl":"https://doi.org/10.3390/microorganisms12122598","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is a human pathogen responsible for a wide range of diseases, such as skin and soft tissue infections, pneumonia, toxic shock syndrome, and urinary tract infections. Methicillin-resistant <i>S. aureus</i> (MRSA) is a well-known pathogen with consistently high mortality rates. Detecting the <i>mecA</i> resistance gene and phenotypical profile to β-lactams allows for the differentiation of MRSA from methicillin-susceptible <i>S. aureus</i> (MSSA) isolates. In this study, we characterized 57 <i>S. aureus</i> clinical isolates for β-lactam susceptibility and <i>mecA</i> presence. We classified 52.63% as MRSA and 45.61% as MSSA. However, some isolates evidenced different oxacillin resistance profiles, such as borderline oxacillin-resistant or modified <i>S. aureus</i> (BORSA/MODSA). The cefazolin inoculum effect (CzIE) was established for these samples, emphasizing the relevance of these isolates as a source of therapeutic failure. We also performed the detection of the Panton-Valentine Leucocidin virulence genes as well as the <i>S. aureus spa</i>-type clonality. As expected, <i>spa</i>-types t002 and t008 were the most prevalent clones, demonstrating the success of well-established clones. These findings emphasize the importance of establishing sensitivity profiles, especially in isolates with poor resistance mechanisms, to determine their prevalence and their impact on public health.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11679903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}