Microorganisms最新文献_第7页

The Effect of Apple and Pear Cultivars on In Vitro Fermentation with Human Faecal Microbiota. 苹果和梨品种对人类粪便微生物群体外发酵的影响。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-11 DOI: 10.3390/microorganisms13081870

Anna M E Hoogeveen, Christine A Butts, Caroline C Kim, Carel M H Jobsis, Shanthi G Parkar, Halina M Stoklosinski, Kevin H Sutton, Patricia Davis, Duncan I Hedderley, Jason Johnston, Pramod K Gopal

{"title":"The Effect of Apple and Pear Cultivars on In Vitro Fermentation with Human Faecal Microbiota.","authors":"Anna M E Hoogeveen, Christine A Butts, Caroline C Kim, Carel M H Jobsis, Shanthi G Parkar, Halina M Stoklosinski, Kevin H Sutton, Patricia Davis, Duncan I Hedderley, Jason Johnston, Pramod K Gopal","doi":"10.3390/microorganisms13081870","DOIUrl":"https://doi.org/10.3390/microorganisms13081870","url":null,"abstract":"Apples and pears are among the most popular and frequently consumed fruits worldwide. The polyphenol and dietary fibre components of these fruits are known to influence the gut microbiota and the subsequent human health outcomes. This study investigated the effects of New Zealand grown apples and pears with differing polyphenol contents on the structure and function of the human gut microbiota. Five apple and two pear cultivars underwent in vitro human digestion and microbial fermentation. Samples taken at 0 and 18 h were analysed for changes in pH, microbial composition, and organic acid production. The change in pH after faecal fermentation was influenced by the type of fruit (apple or pear), with lower pH being observed in the apples. Significant apple or pear cultivar effects were observed for the gut microbiome and organic acid production. The apple cultivar 'Golden Hornet' produced the least butyrate and the greatest microbial alpha diversity, while the pear 'PremP009' showed greater butyrate production with increases in a butyrogenic species (Acidaminococcus intestini). Further studies are needed to investigate the effect of cultivar and type of fruit on nutrient absorption and microbial fermentation and the impact of these on human health.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serrapeptase Eliminates Escherichia coli Biofilms by Targeting Curli Fibers, Lipopolysaccharides, and Phosphate Metabolism. Serrapeptase通过靶向Curli纤维，脂多糖和磷酸盐代谢消除大肠杆菌生物膜。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-11 DOI: 10.3390/microorganisms13081875

Georgios Katsipis, Michalis Aivaliotis, Anastasia A Pantazaki

{"title":"Serrapeptase Eliminates Escherichia coli Biofilms by Targeting Curli Fibers, Lipopolysaccharides, and Phosphate Metabolism.","authors":"Georgios Katsipis, Michalis Aivaliotis, Anastasia A Pantazaki","doi":"10.3390/microorganisms13081875","DOIUrl":"https://doi.org/10.3390/microorganisms13081875","url":null,"abstract":"Escherichia coli biofilms are implicated in the development of persistent infections and increased antibiotic resistance, posing a significant challenge in clinical settings. These biofilms enhance bacterial survival by forming protective extracellular matrices, rendering conventional treatments less effective. Serrapeptase (SPT), a proteolytic enzyme, has emerged as a potential anti-biofilm agent due to its ability to degrade biofilm components and disrupt bacterial adhesion. In this study, we report the inhibitory effect of SPT against E. coli biofilm and its effect on key virulence factors. In vitro assays, including crystal violet staining, optical and fluorescence microscopy, and viability measurements, revealed the dose-dependent inhibition of biofilm formation (IC50 = 14.2 ng/mL), reduced biofilm (-92%, 500 ng/mL) and planktonic viability (-45%, 500 ng/mL), and a marked loss of amyloid curli fibers. SPT treatment also lowered the levels of key virulence factors: cellular and secreted lipopolysaccharides (-76%, 8 ng/mL; -94%, 32 ng/mL), flagellin (-63%, 8 ng/mL), and peptidoglycan (-29%, 125 ng/mL). Mechanistically, SPT induced a phosphate-dysregulating response: secreted alkaline phosphatase activity rose (+70%, 125 ng/mL) while cellular DING/PstS proteins declined (-84%, 64 ng/mL), correlating strongly with biofilm inhibition. In silico docking further suggests direct interactions between SPT and the curli subunits CsgA and CsgB, potentially blocking fiber polymerization. Together, these findings position SPT as a powerful non-antibiotic biofilm disruptor against E. coli, offering a promising strategy to undermine bacterial persistence and resistance by targeting both structural matrix components and metabolic regulatory pathways.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Changes in the Epidemiology of Group A Streptococcus Infections: Observations and Implications. A群链球菌感染流行病学的最新变化：观察和启示。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-11 DOI: 10.3390/microorganisms13081871

Susanna Esposito, Marco Masetti, Carolina Calanca, Nicolò Canducci, Sonia Rasmi, Alessandra Fradusco, Nicola Principi

{"title":"Recent Changes in the Epidemiology of Group A Streptococcus Infections: Observations and Implications.","authors":"Susanna Esposito, Marco Masetti, Carolina Calanca, Nicolò Canducci, Sonia Rasmi, Alessandra Fradusco, Nicola Principi","doi":"10.3390/microorganisms13081871","DOIUrl":"https://doi.org/10.3390/microorganisms13081871","url":null,"abstract":"Streptococcus pyogenes (Group A Streptococcus, GAS) is a major human pathogen capable of causing infections ranging from mild pharyngitis and impetigo to severe invasive diseases such as bacteremia, necrotizing fasciitis, and streptococcal toxic shock syndrome (STSS). Historically, the incidence of GAS infections declined during the early antibiotic era but began rising again from the early 2000s, driven partly by the emergence of hyper-virulent strains such as emm1 and emm12. From 2005 onward, significant increases in GAS infections were reported globally, accompanied by rising antibiotic resistance, particularly to macrolides and tetracyclines. During the COVID-19 pandemic, widespread public health measures led to a sharp decline in GAS infections, including invasive cases, but this trend reversed dramatically in late 2022 and 2023, with surges exceeding pre-pandemic levels, notably in children. Recent data implicate factors such as \"immunity debt,\" viral co-infections, and the spread of virulent clones like M1UK. Looking forward, continued surveillance of GAS epidemiology, virulence factors, and resistance patterns is critical. Moreover, the emergence of GAS isolates with reduced susceptibility to beta-lactams underscores the need for vigilance despite the absence of fully resistant strains. The development of an effective vaccine remains an urgent priority to reduce GAS disease burden and prevent severe outcomes. Future research should focus on vaccine development, molecular mechanisms of virulence, and strategies to curb antimicrobial resistance.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification and Comparison of Different Biofilm Components from Methicillin-Susceptible Staphylococcus aureus Treated with Tranexamic Acid Using an In Vitro Model. 氨甲环酸处理甲氧西林敏感金黄色葡萄球菌不同生物膜成分的体外模型定量比较

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-11 DOI: 10.3390/microorganisms13081874

Marta Díaz-Navarro, Antonio Benjumea, Andrés Visedo, Patricia Muñoz, Javier Vaquero, Francisco Chana, María Guembe

{"title":"Quantification and Comparison of Different Biofilm Components from Methicillin-Susceptible Staphylococcus aureus Treated with Tranexamic Acid Using an In Vitro Model.","authors":"Marta Díaz-Navarro, Antonio Benjumea, Andrés Visedo, Patricia Muñoz, Javier Vaquero, Francisco Chana, María Guembe","doi":"10.3390/microorganisms13081874","DOIUrl":"https://doi.org/10.3390/microorganisms13081874","url":null,"abstract":"As we previously demonstrated that tranexamic acid (TXA), an antifibrinolytic, showed an antibacterial effect alone and in combination with vancomycin and gentamicin, we now wanted to analyze its own efficacy using new, different fluorescent staining reagents that target different components of the biofilm matrix and compare which one quantifies biofilm reduction better. A 108 cfu/mL suspension of the Staphylococcus aureus (ATCC29213) strain was placed into the wells of a 24-multiwell plate covered with glass slides coated with 10% poly-L-lysine under agitation for 24 h at 37 °C. After 3 washes with PBS, wells were treated with either TXA 10 mg/mL or sterile water and incubated for 24 h at 37 °C. After three washes with PBS, the density area of the following biofilm components was calculated using confocal laser scanning microscopy: extracellular proteins (Sypro Ruby), α-extracellular polysaccharides (ConA-Alexa fluor 633), α or β-extracellular polysaccharides (GS-II-Alexa fluor 488), bacterial DNA (PI), and eDNA (TOTO®-1). We observed a statistically significant reduction in the occupied area by all components of the S. aureus biofilm (p < 0.001) after TXA 10 mg/mL treatment, compared to the positive control. All biofilm components' reduction percentages reached ≥90.0%. We demonstrated that TXA reduced both bacteria and extracellular matrix components of S. aureus biofilm by using five different stain reagents, with all being equally valid for quantification.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burkholderia Phages and Control of Burkholderia-Associated Human, Animal, and Plant Diseases. 伯克氏菌噬菌体与与伯克氏菌相关的人、动物和植物疾病的控制。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-11 DOI: 10.3390/microorganisms13081873

Bingjie Wang, Jiayi Zhang, Lei Chen, Munazza Ijaz, Ji'an Bi, Chenhao Li, Daixing Dong, Yanxin Wang, Bin Li, Jinyan Luo, Qianli An

引用次数: 0

Combating Antimicrobial Resistance: Innovations and Strategies. 抗微生物药物耐药性：创新和战略。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-11 DOI: 10.3390/microorganisms13081869

Vasiliki Koumaki

引用次数: 0

Widespread Presence of SPX and Its Potential Role as a Phosphorus Nutrient Regulator in Dinoflagellates. SPX在鞭毛藻中的广泛存在及其作为磷营养调节剂的潜在作用。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-10 DOI: 10.3390/microorganisms13081867

Jiashun Li, Jingtian Wang, Xiaoyu Wang, Kaidian Zhang, Senjie Lin

{"title":"Widespread Presence of SPX and Its Potential Role as a Phosphorus Nutrient Regulator in Dinoflagellates.","authors":"Jiashun Li, Jingtian Wang, Xiaoyu Wang, Kaidian Zhang, Senjie Lin","doi":"10.3390/microorganisms13081867","DOIUrl":"https://doi.org/10.3390/microorganisms13081867","url":null,"abstract":"SPX domain-containing proteins (SPXc) are crucial for regulating phosphorus (P) homeostasis in plants. Recently, the SPX gene was identified in the diatom model Phaeodactylum tricornutum and shown to serve as a negative regulator of P acquisition. Whether SPXc occurs in dinoflagellates is unclear. Here, we report the presence and potential functions of genes encoding SPXc in dinoflagellates (dino-SPXc). Four classes of SPXc were identified in dinoflagellates, including the three known classes-the stand-alone SPX, SPX-EXS, and SPX-VTC-and SPX-other, with SPX and SPX-EXS being dominant. Using the TARA Oceans database, we investigated the taxonomic and geographic distributions of dino-SPXc and found variations in dino-SPXc expression among size classes of dinoflagellates. The harmful algal bloom-causative species Prorocentrum shikokuense possesses all four classes of SPXc proteins, showing a fluctuating expression pattern under different nutrient conditions and during different phases of the cell cycle and algal bloom. In addition, the SPXc genes in Symbiodiniaceae respond not only to P stress but also to thermal variations. These results are consistent with a role of dino-SPXc in maintaining P homeostasis in dinoflagellates and suggest the importance of SPX-related genes in enabling dinoflagellates to sustain population growth in nutrient-variable oceans, warranting further research.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BST-2 Promotes N Protein Degradation and Inhibits Viral Replication Through the MARCHF8/NDP52 Autophagy Pathway. BST-2通过MARCHF8/NDP52自噬途径促进N蛋白降解并抑制病毒复制

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-09 DOI: 10.3390/microorganisms13081865

Chenchen Zhao, Yan Qin, Haixin Huang, Yuying Li, Xinyu Zhang, Lin Zhou, Lulu Xie, Yimin Zhou, Yanqing Hu, Wei Chen, Tian Lan, Wen-Chao Sun

{"title":"BST-2 Promotes N Protein Degradation and Inhibits Viral Replication Through the MARCHF8/NDP52 Autophagy Pathway.","authors":"Chenchen Zhao, Yan Qin, Haixin Huang, Yuying Li, Xinyu Zhang, Lin Zhou, Lulu Xie, Yimin Zhou, Yanqing Hu, Wei Chen, Tian Lan, Wen-Chao Sun","doi":"10.3390/microorganisms13081865","DOIUrl":"https://doi.org/10.3390/microorganisms13081865","url":null,"abstract":"Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a recently discovered enteric coronavirus that has caused considerable economic losses in the pig industry. SADS-CoV was first reported in 2017 in Guangdong Province, China, and subsequently in Fujian, Guangxi, Henan and Jiangxi Provinces. Bone marrow stromal cell antigen 2 (BST-2), also known as tetherin, acts as an antiviral protein to limit the release of a wide range of enveloped viruses. However, the relationship between BST-2 and SADS-CoV has rarely been studied. Here, we showed that endogenous BST-2 expression is downregulated by SADS-CoV infection in Vero-E6 and ST cells by 2- to 3-fold. The overexpression of BST-2 inhibited SADS-CoV replication, whereas the knockdown of the BST-2 gene in Vero cells restored SADS-CoV replication. Further study revealed that BST-2 targets the SADS-CoV nucleocapsid protein (N) and decreases N protein expression, and that the BST-2 transmembrane (TM) domain is essential for this activity. Moreover, the degradation of the SADS-CoV N protein promoted by BST-2 is mediated by the membrane-associated ring-CH-type finger 8 (MARCHF8)/calcium binding and coiled-coil domain 2 (NDP52) autophagosome pathway. Overall, we found that BST-2 suppresses viral proliferation by inducing the breakdown of the SADS-CoV N protein via the MARCHF8/NDP52 pathway.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Desulfitobacterium elongatum sp. nov. NIT-TF6 Isolated from Trichloroethene-Dechlorinating Culture with Formate. 从甲酸三氯乙烯脱氯培养中分离得到的长形脱硫菌nni - tf6。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-09 DOI: 10.3390/microorganisms13081863

Udaratta Bhattacharjee, Ryuya Tomita, Li Xie, Naoko Yoshida

{"title":"Desulfitobacterium elongatum sp. nov. NIT-TF6 Isolated from Trichloroethene-Dechlorinating Culture with Formate.","authors":"Udaratta Bhattacharjee, Ryuya Tomita, Li Xie, Naoko Yoshida","doi":"10.3390/microorganisms13081863","DOIUrl":"https://doi.org/10.3390/microorganisms13081863","url":null,"abstract":"A strictly anaerobic bacterium denoted as strain NIT-TF6 of the genus Desulfitobacterium was isolated from a trichloroethene-dechlorinating culture with formate. Cells were straight rods of 1.6-6 µm long and 0.25-0.5 µm in diameter and used H2, lactate, pyruvate, and malate as electron donors and thiosulfate and Fe (III)-citrate as electron acceptors. The genome of strain NIT-TF6 was 4.8 Mbp in size and included nine 16S rRNA genes. Phylogenetic analysis based on 16S rRNA sequences showed that NIT-TF6 shared the highest sequence similarity (96.39%) with Desulfitobacterium hafniense DCB-2ᵀ, forming an independent clade in the phylogenetic tree. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain NIT-TF6 and other Desulfitobacterium species ranged from 15.9 to 16.9% and from 71.68 to 72.51%, respectively. These are well below the thresholds for species delineation. A distinguishing feature of strain NIT-TF6 was its possession of both L-lactate dehydrogenase (L-LDH) and D-lactate dehydrogenase (D-LDH), in contrast to other Desulfitobacterium strains that exclusively express D-LDH. Based on the dDDH and ANI results, combined with physiological, phylogenetic, morphological, biochemical, genomic, and metabolic iron-related characteristics, strain NIT-TF6 has been proposed as a novel species within the genus Desulfitobacterium. The name Desulfitobacterium elongatum sp. nov. has been proposed for this strain, with NIT-TF6ᵀ designated as the type strain.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Characterization of Marine Staphylococcus shinii Strain SC-M1C: Potential Genetic Adaptations and Ecological Role. 海洋希氏葡萄球菌SC-M1C的基因组特征：潜在的遗传适应和生态作用

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-08-09 DOI: 10.3390/microorganisms13081866

Manar El Samak, Hasnaa Lotfy, Abdelrahman M Sedeek, Yehia S Mohamed, Samar M Solyman

{"title":"Genomic Characterization of Marine Staphylococcus shinii Strain SC-M1C: Potential Genetic Adaptations and Ecological Role.","authors":"Manar El Samak, Hasnaa Lotfy, Abdelrahman M Sedeek, Yehia S Mohamed, Samar M Solyman","doi":"10.3390/microorganisms13081866","DOIUrl":"https://doi.org/10.3390/microorganisms13081866","url":null,"abstract":"Staphylococcus shinii (S. shinii) is a coagulase-negative species primarily associated with the degradation of organic matter, contributing to nutrient cycling in natural environments. This species has been mainly studied in clinical and terrestrial contexts, with no previous reports of its presence in marine environments. In this study, we report the first isolation of S. shinii from a marine habitat. The strain SC-M1C was isolated from the Red Sea sponge Negombata magnifica. Whole-genome sequencing confirmed its taxonomic identity as S. shinii. The genome uncovers potential adaptive characteristics that facilitate survival in marine ecosystems, comprising genes associated with osmoregulation, nutrient acquisition, stress response, and resistance to heavy metals. Moreover, multiple genomic islands and plasmids were identified, suggesting a potential role in horizontal gene transfer and environmental adaptability. The presence of biosynthetic gene clusters linked to non-ribosomal peptides, siderophores, and terpene production indicates potential for biochemical versatility beyond traditional metabolic expectations. This study presents the first genomic insights into S. shinii in a marine context, highlighting its ecological significance and adaptive mechanisms in a high-salinity environment. These findings expand our understanding of staphylococcal ecology beyond terrestrial and clinical origins and provide a foundation for exploring the role of S. shinii in marine microbial interactions and environmental resilience.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 8","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12388102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0