Microorganisms最新文献_第7页

Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria. 保加利亚HIV-1亚型A1、A6和A7的不同分子流行病学、传播模式和耐药性突变

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-12 DOI: 10.3390/microorganisms13051108

Aleksandra Partsuneva, Anna Gancheva, Reneta Dimitrova, Lyubomira Grigorova, Asya Kostadinova, Maria Nikolova, Radoslava Emilova, Nina Yancheva, Rusina Grozdeva, Ivailo Alexiev

{"title":"Distinct Molecular Epidemiology, Transmission Patterns, and Resistance Mutations of HIV-1 Subtypes A1, A6, and A7 in Bulgaria.","authors":"Aleksandra Partsuneva, Anna Gancheva, Reneta Dimitrova, Lyubomira Grigorova, Asya Kostadinova, Maria Nikolova, Radoslava Emilova, Nina Yancheva, Rusina Grozdeva, Ivailo Alexiev","doi":"10.3390/microorganisms13051108","DOIUrl":"10.3390/microorganisms13051108","url":null,"abstract":"By 2022, Bulgaria's National Reference Laboratory had confirmed 4024 HIV cases. We analyzed 132 pol gene sequences to characterize the molecular epidemiology of HIV-1 subtypes A1, A6, and A7 (2001-2022). A1 accounted for 50.0% (66/132) of cases, increasing after 2014, with peaks in 2019 and 2022. A6 comprised 48.5% (64/132), dominating from 2005 to 2014 before stabilizing. A7 was rare (1.5%, 2/132), detected in 2003 and 2011. Transmission patterns varied: A1 was linked to men who have sex with men (MSM) (62.1%), while A6 was primarily heterosexual (HET) (82.8%) with a balanced gender distribution (56.3% male, 43.8% female). Resistance mutations were identified in 29.6% of cases, with A6 showing higher rates of nucleoside reverse transcriptase inhibitor (NRTI) (20.3%) and non-nucleoside reverse transcriptase inhibitor (NNRTI) (7.8%) resistance than A1. Phylogenetic analysis revealed that 13 Bulgarian sequences (9.8%) were involved in transmission clusters, including 10 (7.6%) from sub-subtype A1 and 3 (2.3%) from sub-subtype A6, highlighting distinct genetic diversity and transmission patterns. Despite significant migration from Ukraine in 2022, A6 prevalence remained unchanged, suggesting localized transmission dynamics. These findings highlight a shifting HIV-1 sub-subtype distribution in Bulgaria and emphasize the need for targeted prevention, diagnosis, and treatment strategies tailored to the evolving molecular landscape.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Community Structure, Assembly and Interactions of Nitrospira Nitrite-Oxidizing Bacteria in Sediments of the Eastern China Marginal Seas. 中国东部边缘海沉积物中亚硝酸盐氧化亚硝基螺旋菌的群落结构、聚集和相互作用。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-12 DOI: 10.3390/microorganisms13051112

Hao Dong, Hui He, Min Wang

{"title":"Community Structure, Assembly and Interactions of Nitrospira Nitrite-Oxidizing Bacteria in Sediments of the Eastern China Marginal Seas.","authors":"Hao Dong, Hui He, Min Wang","doi":"10.3390/microorganisms13051112","DOIUrl":"10.3390/microorganisms13051112","url":null,"abstract":"Nitrite oxidation, a pivotal process in the nitrogen cycle driven by microorganisms, is primarily carried out by nitrite-oxidizing bacteria (NOB). While extensive studies on Nitrospira have been conducted in terrestrial habitats, knowledge of Nitrospira in marine sediments remains limited. Therefore, we employed high-throughput sequencing analysis to systematically explore the community structure, assembly processes and potential interactions of Nitrospira in the eastern China marginal seas. Our results exhibit pronounced spatial heterogeneity in Nitrospira communities across seas. Widespread distribution of Nitrospira taxa was observed, with Nitrospira lineage II emerging as the most important group in this study. Based on the neutral community model (NCM), normalized stochasticity ratio (NST) and beta nearest-taxon-index (βNTI) analysis, deterministic processes predominantly shaped the community assembly of Nitrospira. Complex interspecies interactions among Nitrospira were observed with molecular ecological network analysis, and the community in the East China Sea showed the highest complexity, while the community displayed the greatest stability in the South Yellow Sea. In addition, the Nitrospira communities were notably influenced by geographic distances and environmental factors, including salinity, temperature, dissolved oxygen concentration and dissolved inorganic nutrient concentration. These results may expand our understanding of Nitrospira in marine environments and enhance our insights into the marine nitrogen cycle.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Funneliformis mosseae and Cu Additives on the Astragalus sinicus Root Growth and Cd Uptake Under the Modeled Conditions. 模拟条件下，苔藓漏斗虫和Cu添加剂对黄芪根系生长和Cd吸收的影响。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-12 DOI: 10.3390/microorganisms13051109

Yuxin Li, Rui Cai, Jindian Hu, Hongling Liu, Xiancan Zhu

{"title":"Effect of Funneliformis mosseae and Cu Additives on the Astragalus sinicus Root Growth and Cd Uptake Under the Modeled Conditions.","authors":"Yuxin Li, Rui Cai, Jindian Hu, Hongling Liu, Xiancan Zhu","doi":"10.3390/microorganisms13051109","DOIUrl":"10.3390/microorganisms13051109","url":null,"abstract":"Cadmium (Cd) contamination in soil poses a serious threat to plant growth and productivity, while arbuscular mycorrhizal (AM) fungi play a vital role in enhancing plant growth, improving tolerance to heavy metals, and restoring polluted ecosystems. To enhance the tolerance of Astragalus sinicus to Cd stress, a pot experiment was conducted to investigate the effects of inoculation and copper (Cu) addition on growth, Cd accumulation, and translocation under Cd-stressed soil conditions. The results showed that Cd inhibited the root growth of A. sinicus, and AM fungi inoculation and Cu + AM significantly increased root biomass and root volume (p < 0.05). Under Cd stress, AM fungi inoculation reduced Cd concentration by 72.40% in the shoots, while it increased by 92.69% in the roots. Both AM fungi inoculation and Cu + AM enhanced Cd uptake in the roots, while inhibiting Cd translocation to the shoots. After the application of Cu and inoculation with AM fungi, the roots have a strong absorption and enrichment ability for Cd; the bioconcentration factor of Cd in the roots of A. sinicus reached 1018.59% and 366.08%, respectively. Cu + AM increased the enrichment of Cd in the roots and restricted its translocation to the shoots. Moreover, the combination of AM fungi inoculation and Cu addition significantly increased soluble sugar (by 77.29%) and proline contents (by 445.62%) and reduced CAT activity (by 74.67%) under Cd stress. In summary, both Cu addition and AM fungi inoculation promoted the growth of A. sinicus under Cd stress, improved its physiological metabolism, and reduced Cd content in the soil, with the combined Cu and AM fungi treatment showing the most significant effect.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microorganisms in Macroalgae Cultivation Ecosystems: A Systematic Review and Future Prospects Based on Bibliometric Analysis. 基于文献计量学分析的大型藻类栽培生态系统微生物研究综述与展望

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-12 DOI: 10.3390/microorganisms13051110

Yinglong Chen, Pengbing Pei, Muhammad Aslam, Muhamad Syaifudin, Ran Bi, Ping Li, Hong Du

{"title":"Microorganisms in Macroalgae Cultivation Ecosystems: A Systematic Review and Future Prospects Based on Bibliometric Analysis.","authors":"Yinglong Chen, Pengbing Pei, Muhammad Aslam, Muhamad Syaifudin, Ran Bi, Ping Li, Hong Du","doi":"10.3390/microorganisms13051110","DOIUrl":"10.3390/microorganisms13051110","url":null,"abstract":"Microorganisms play an essential role in the biogeochemical processes of macroalgal cultivation ecosystems by participating in a complex network of interactions, significantly influencing the growth and development of macroalgae. This study used bibliometric analysis and VOSviewer based on Web of Science data to provide an overview by tracing the developmental footprint of the technology. Countries, institutions, authors, keywords, and key phrases were tracked and mapped accordingly. From 1 January 2003 to 31 December 2023, 619 documents by 2516 authors from 716 institutions in 51 countries were analyzed. Keyword co-occurrence network analysis revealed five main areas of research on microbes in macroalgal cultivation ecosystems: (1) identification of microbial species and functional genes, (2) biogeochemical cycling of carbon in microbial communities, (3) microbial influences on macroalgae growth and development, (4) bioactivities, and (5) studies based on database. Thematic evolution and map research emphasized the centrality of microbial diversity research in this direction. Over time, the research hotspots and the core scientific questions of the microorganisms in the macroalgal cultivation ecosystems have evolved from single-organism interactions to the complex dynamics of microbial communities. The application of high-throughput techniques had become a hotspot, and the adoption of systems biology approaches had further facilitated the integrated analysis of microbial community composition and function. Our results provide valuable guidance and information for future researches on algal-bacterial interactions and microbe-driven carbon cycling in coastal ecosystems.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Widespread Detection of Fowl Adenovirus Serotype 2/11 Species D Among Cases of Inclusion Body Hepatitis-Hydropericardium Syndrome in Chickens in Egypt. 埃及鸡包涵体肝炎心包积液综合征病例中禽腺病毒2/11种D型的广泛检测

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-12 DOI: 10.3390/microorganisms13051107

Doaa M Abdellatif, Azza A El-Sawah, Magdy F Elkady, Ahmed Ali, Khaled Abdelaziz, Salama A S Shany

{"title":"Widespread Detection of Fowl Adenovirus Serotype 2/11 Species D Among Cases of Inclusion Body Hepatitis-Hydropericardium Syndrome in Chickens in Egypt.","authors":"Doaa M Abdellatif, Azza A El-Sawah, Magdy F Elkady, Ahmed Ali, Khaled Abdelaziz, Salama A S Shany","doi":"10.3390/microorganisms13051107","DOIUrl":"10.3390/microorganisms13051107","url":null,"abstract":"Fowl adenoviruses (FAdVs) are important emerging pathogens affecting the poultry industry in Egypt as they are the primary etiology of inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) associated with severe economic losses. This study aims to identify the circulating FAdVs from cases of IBH-HPS in 5 Egyptian provinces during the period from October 2020 through September 2022. Out of the 210 examined flocks, liver samples from 66 flocks were positive for FAdVs (31.4%) using conventional polymerase chain reaction targeting loop 1 of the major hexon gene, with varying rates of mortality (1% to 14%). In the positive samples detected during the study, the histopathological examination revealed pathognomonic lesions of FAdVs, including basophilic and eosinophilic intra-nuclear inclusion bodies (INIBs). The percentage of FAdV positivity increased with the flock age; from samples collected at ages 1 to10, 11 to 20, 21 to 30, and >30 days of age, 10% (5/50), 25.6% (11/43), 34.3% (23/67), and 54% (27/50) were found positive for FAdVs, respectively. Notably, the positivity percentages among the flocks reared in cages were higher than for those reared in the deep litter system of housing. The gene sequencing and phylogenetic analysis of 19 strains revealed clustering into FAdV species D serotype 2/11, demonstrating that serotype 2/11 is most prevalent in the targeted Egyptian provinces during the period of the study. Several point mutations in the sequenced region among different strains were reported. These findings underscore the prevalence of FAdV and provide a basis for further research on circulating strains to develop effective control strategies.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil Native C/N Ratio Affects Diazotrophic Bacterial Composition and N Fixation by Regulating SOC Distribution in Soil Particles After Residue Incorporation. 土壤天然碳氮比通过调节土壤颗粒中有机碳分布影响重氮营养细菌组成和固氮作用。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-11 DOI: 10.3390/microorganisms13051104

Pengfei Duan, Di Zhao, Shuqiong Yang, Jibao Chen, Zhaojin Chen, Lingling Cao

{"title":"Soil Native C/N Ratio Affects Diazotrophic Bacterial Composition and N Fixation by Regulating SOC Distribution in Soil Particles After Residue Incorporation.","authors":"Pengfei Duan, Di Zhao, Shuqiong Yang, Jibao Chen, Zhaojin Chen, Lingling Cao","doi":"10.3390/microorganisms13051104","DOIUrl":"10.3390/microorganisms13051104","url":null,"abstract":"Residue incorporation is a universally accepted agronomic practice around the world. However, the relationship between C and N turnover in soil is still not fully understood. We performed a soil microcosm incubation experiment to study the effects of residue incorporation with different C-to-N ratios (C/N) (low, 31.8; high, 84.5) on the SOC distribution, diazotrophic bacterial composition, and N accumulation in different particle size fractions (PSFs) of three types of soil parent material with different native C/N ratios. Our results demonstrate that SOC is 1.34 to 2.50 times more easily retained and accumulated in clay compared to baseline levels at the early stage of soil development. The native C/N ratio markedly affects the microbial metabolic characteristics and the SOC accumulation processes. Specifically, low C/N residue enhanced SOC accumulation in high-native-C/N soil, while the high-C/N residue maximized SOC in low-native-C/N soil. These shifts regulated the composition of the diazotrophic bacterial community, increasing the nitrogenase coding gene (nifH) abundance 5.57- and 8.1-fold in low-C/N residue treatments and 6.6-fold in the high-C/N residue treatment, thereby promoting soil N accumulation. Moreover, we highlight the potential role of Bradyrhizobiaceae, Rhodospirillaceae, Micrococcaceae, Rhizobiaceae, Comamonadaceae, Nitrospiraceae, and Burkholderiaceae in biological N fixation during pedogenesis. This study explored the coupling relationship between soil native and residue C/N and soil biological N fixation at the soil particle level, and the results suggest that residue incorporation to improve soil fertility needs to be further explored according to soil native C/N.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Meso- and Bathypelagic Archaeal and Bacterial Communities of the Southern Gulf of Mexico Are Dominated by Nitrifiers and Hydrocarbon Degraders. 墨西哥湾南部中深海古细菌和细菌群落以硝化菌和烃类降解菌为主。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-11 DOI: 10.3390/microorganisms13051106

Lizt Selene Osorio-Pando, Mario Hernández-Guzmán, Karla Sidón-Ceseña, Yamne Ortega-Saad, Victor F Camacho-Ibar, Jennyfers Chong-Robles, Asunción Lago-Lestón

{"title":"The Meso- and Bathypelagic Archaeal and Bacterial Communities of the Southern Gulf of Mexico Are Dominated by Nitrifiers and Hydrocarbon Degraders.","authors":"Lizt Selene Osorio-Pando, Mario Hernández-Guzmán, Karla Sidón-Ceseña, Yamne Ortega-Saad, Victor F Camacho-Ibar, Jennyfers Chong-Robles, Asunción Lago-Lestón","doi":"10.3390/microorganisms13051106","DOIUrl":"10.3390/microorganisms13051106","url":null,"abstract":"The Gulf of Mexico (GoM) is a complex oceanic basin with a maximum depth of 4000 m. It is a complex hydrodynamic system formed by different water masses with distinctive physical and biological characteristics that shape its rich biodiversity. In this study, as a contribution to better understanding the microbial communities inhabiting the meso- and bathypelagic zones of the Mexican Exclusive Economic Zone (EEZ) of the GoM, an extensive set of seawater samples was collected at three depths (350-3700 m) during three oceanographic cruises. The V4-16S rRNA gene analysis identified Pseudomonadota (27.1 ± 9.8%) and Nitrosopumilales (26.4 ± 2.3%) as the dominant bacterial and archaeal members, respectively. The depth, salinity, and apparent oxygen utilization were key environmental drivers, which explained 35% of the community variability. The mesopelagic zone presented a more homogeneous structure characterized by a nitrifier community, while the bathypelagic was more heterogeneous, with hydrocarbon-degrading bacteria and methanogens serving as the key players. This study is the first to report the archaeal community in the deeper waters of the Mexican EEZ of the GoM, playing crucial roles in the nitrogen and carbon cycles, highlighting the region's ecological complexity and the need for further research to understand the broader biogeochemical implications of these processes.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remarks on Life Feasibility on the Red Planet. 关于火星上生命存在可能性的评论。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-11 DOI: 10.3390/microorganisms13051105

Fiorella Mancini, Giuseppe Graziano

引用次数: 0

Ureolysis-Driven Microbially Induced Carbonate Precipitation by a Facultatively Anaerobic Thermophilic Bacterium Under High-Temperature and Anaerobic Conditions. 高温厌氧条件下嗜热兼性厌氧细菌解尿驱动微生物诱导碳酸盐沉淀。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-10 DOI: 10.3390/microorganisms13051102

Xiulun Shen, Sijia He, Yutaro Takaya, Tomoyoshi Yakata, Kotaro Yoshida, Hajime Kobayashi

{"title":"Ureolysis-Driven Microbially Induced Carbonate Precipitation by a Facultatively Anaerobic Thermophilic Bacterium Under High-Temperature and Anaerobic Conditions.","authors":"Xiulun Shen, Sijia He, Yutaro Takaya, Tomoyoshi Yakata, Kotaro Yoshida, Hajime Kobayashi","doi":"10.3390/microorganisms13051102","DOIUrl":"10.3390/microorganisms13051102","url":null,"abstract":"Microbially induced carbonate precipitation (MICP) is the precipitation of CaCO3 crystals, induced by microbial metabolic activities such as ureolysis. Various applications of MICP have been proposed as innovative biocementation techniques. This study aimed to verify the feasibility of ureolysis-driven MICP applications in deep-subsurface environments (e.g., enhanced oil recovery and geological carbon sequestration). To this end, we screened sludge collected from a high-temperature anaerobic digester for facultatively anaerobic thermophilic bacteria possessing ureolytic activity. Then, we examined the ureolysis-driven MICP using a representative isolate, Bacillus haynesii strain SK1, under aerobic, anoxic, and strict anaerobic conditions at 30 °C, 40 °C, and 50 °C. All cultures showed ureolysis and the formation of insoluble precipitates. Fourier transform infrared spectroscopy analysis revealed precipitates comprising CaCO3 at 30 °C, 40 °C, and 50 °C under aerobic conditions but only at 50 °C under anoxic and strict anaerobic conditions, suggesting efficient MICP at 50 °C. Interestingly, an X-ray diffraction analysis indicated that calcium carbonate crystals that were produced under aerobic conditions were in the form of calcite, while those that were produced under anoxic and strict anaerobic conditions at 50 °C were mostly in the form of vaterite. Thus, we demonstrated ureolysis-driven MICP under high-temperature and O2-depletion conditions, suggesting the potential of MICP applications in deep-subsurface environments.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, Identification, and Control of Pathogenic Endophytic Fungi in Nymphaea candida Presl Tissue Culture. 念珠莲组织培养病原菌的分离、鉴定及防治。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-10 DOI: 10.3390/microorganisms13051103

Yuwei Xing, Cong Liu, Xumeng Cui, Haonan Lv, Jun Wang

{"title":"Isolation, Identification, and Control of Pathogenic Endophytic Fungi in Nymphaea candida Presl Tissue Culture.","authors":"Yuwei Xing, Cong Liu, Xumeng Cui, Haonan Lv, Jun Wang","doi":"10.3390/microorganisms13051103","DOIUrl":"10.3390/microorganisms13051103","url":null,"abstract":"Nymphaea candida Presl is a rare hardy water lily at risk of extinction and has been included on the 'Red List' of threatened species of the International Union for the Conservation of Nature. To protect germplasm resources and propagate N. candida seedlings, this study conducted tissue culture and found that pathogenic endophytic fungal infection was the main reason for failure of tissue culture. Compared with the stems and leaves, the roots of N. candida had the highest rates of fungal infection during tissue culture. Subsequently, three isolated endophytic fungi, Fusarium oxysporum, Phytopythium helicoides, and Alternaria sp., showed the highest frequency of occurrence in tissue culture. Furthermore, an antifungal formulation comprising 0.1 μg/mL pyrimidin suspension, 1 μg/mL mancozeb wettable powder, and 1 μg/mL carbendazim was constructed and could reduce the infection rates of root and stem tissues to 7.5% and 0%, respectively. Finally, the usefulness of this antifungal formulation for inhibition of endophytic fungi in tissue culture of N. candida was validated. This study not only provides important technical support for mass production of seedlings of N. candida, but also provides a scientific reference for the protection of endangered aquatic plant species.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0