Microorganisms最新文献

{"title":"Immunosenescence: How Aging Increases Susceptibility to Bacterial Infections and Virulence Factors.","authors":"Nikolaos Theodorakis, Georgios Feretzakis, Christos Hitas, Magdalini Kreouzi, Sofia Kalantzi, Aikaterini Spyridaki, Zoi Kollia, Vassilios S Verykios, Maria Nikolaou","doi":"10.3390/microorganisms12102052","DOIUrl":"https://doi.org/10.3390/microorganisms12102052","url":null,"abstract":"<p><p>The process of aging leads to a progressive decline in the immune system function, known as immunosenescence, which compromises both innate and adaptive responses. This includes impairments in phagocytosis and decreased production, activation, and function of T- and B-lymphocytes, among other effects. Bacteria exploit immunosenescence by using various virulence factors to evade the host's defenses, leading to severe and often life-threatening infections. This manuscript explores the complex relationship between immunosenescence and bacterial virulence, focusing on the underlying mechanisms that increase vulnerability to bacterial infections in the elderly. Additionally, it discusses how machine learning methods can provide accurate modeling of interactions between the weakened immune system and bacterial virulence mechanisms, guiding the development of personalized interventions. The development of vaccines, novel antibiotics, and antivirulence therapies for multidrug-resistant bacteria, as well as the investigation of potential immune-boosting therapies, are promising strategies in this field. Future research should focus on how machine learning approaches can be integrated with immunological, microbiological, and clinical data to develop personalized interventions that improve outcomes for bacterial infections in the growing elderly population.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance Evaluation of Novaplex^TM Multiplex Real-Time PCR Assay for Detection of <i>Streptococcus agalactiae</i> Serotypes.","authors":"Mallikarjun Handigund, Jaehyeon Lee","doi":"10.3390/microorganisms12102043","DOIUrl":"https://doi.org/10.3390/microorganisms12102043","url":null,"abstract":"<p><p><i>Streptococcus agalactiae</i>, or group B streptococcus (GBS), is a Gram-positive pathogen with an extended track record of colonization in the gastrointestinal and genitourinary tracts. GBS can induce disease in individuals across all age demographics, yet it predominantly triggers infections in neonates and the elderly. Identification of the serotype is vital for effective management of the disease as it provides critical information for clinicians on the cause of the disease. In this study, we evaluated the rapid, simple, and easy-to-adopt multiplex real-time PCR technique, Novaplex^TM (NovaPCR). A total of 131 clinical isolates of different serotypes were tested using NovaPCR. Observations revealed that 129 isolates showed the same observations as LA and conventional mPCR. NovaPCR accurately identified serotypes IV and V, which were first classified as serotype Ia in the LA test and mPCR, and the difference between the traditional (LA test and mPCR) and NovaPCR methods is only 1.52%. Accurate serotype identification is helpful for monitoring the epidemics and achieving optimal clinical outcomes, and NovaPCR showed a reliable, fast, easy-to-interpret, and cost-efficient performance in GBS serotyping.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of <i>Bacillus altitudinis</i> 5-DSW with Protease Activity from Deep-Sea Mineral Water and Preparation of Functional Active Peptide Fractions from Chia Seeds.","authors":"Hao Jin, Eun-Gyo Lee, Faiza Khalid, Seung-Wha Jo, Sang-Ho Baik","doi":"10.3390/microorganisms12102048","DOIUrl":"https://doi.org/10.3390/microorganisms12102048","url":null,"abstract":"<p><p>In this study, we successfully isolated <i>Bacillus</i> strains with high protease activity from deep-sea mineral water in Korea and used them to obtain functional peptide fractions from chia seeds. The obtained <i>Bacillus</i> strains showed a high similarity of 99% with <i>B. altitude</i> with a long rod type (named <i>B. altitudinis</i> 5-DSW) and high protease activity at 40 °C, and 70% of the activity remained even at 70 °C. The defatted chia seed protein (15-50 kDa) was treated with crude protease from <i>B. altitudinis</i> 5-DSW and digested into small peptides below 20 kDa. The obtained chia seed peptides showed 3 times and 1.5 times higher antioxidant activity in DPPH and ABT radical scavenging assays, respectively. Moreover, chia seed peptides showed enhanced AChE inhibitory activity with an IC₅₀ value of 14.48 ± 0.88 μg/mL and BChE inhibition activity with an IC₅₀ value of 10.90 ± 0.80 μg/mL. Our results indicate that the newly isolated <i>B. altitudinis</i> 5-DSW and chia seed protein hydrolysates have potential applications in biotechnology and functional food development, enhancing the nutritional quality and value-added utilization of chia byproducts.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbohydrate Metabolism Differentiates <i>Pectinatus</i> and <i>Megasphaera</i> Species Growing in Beer.","authors":"Manuel J Arnold, Stefan W Ritter, Matthias A Ehrmann, Yohanes N Kurniawan, Koji Suzuki, Thomas M Becker, Wolfgang Liebl","doi":"10.3390/microorganisms12102045","DOIUrl":"https://doi.org/10.3390/microorganisms12102045","url":null,"abstract":"<p><p>Obligate anaerobic beer spoilage bacteria have been a menace to the brewing industry for several decades. Technological advances in the brewing process aimed at suppressing aerobic spoilers gave rise to problems with obligate anaerobes. In previous studies, the metabolic spectrum of <i>Pectinatus</i> and <i>Megasphaera</i> species has been described, but their metabolism in the beer environment remains largely unknown. We used high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS) to further characterize beer spoiled by 30 different strains from six beer-spoiling species of <i>Pectinatus</i> and <i>Megasphaera</i> (<i>P. cerevisiiphilus</i>, <i>P. frisingensis</i>, <i>P. haikarae</i>, <i>M. cerevisiae</i>, <i>M. paucivorans</i>, and <i>M. sueciensis</i>). We detected differences in carbohydrate utilization and the volatile organic compounds (volatilome) produced during beer spoilage by all six species. We were able to show that glycerol, one of the basic components of beer, is the common carbon source used by all strains. It appears that this carbon source allows for anaerobic beer spoilage by <i>Pectinatus</i> and <i>Megasphaera</i> despite the spoilage-preventing intrinsic barriers of beer (iso-α-acids, ethanol, low pH, scarce nutrients); thus, extrinsic countermeasures are key for prevention.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into <i>Porphyromonas somerae</i> in Bladder Cancer Patients: Urinary Detection by ddPCR.","authors":"Filippo Russo, Speranza Esposito, Lorella Tripodi, Savio Domenico Pandolfo, Achille Aveta, Felice Amato, Carmela Nardelli, Ciro Imbimbo, Lucio Pastore, Giuseppe Castaldo","doi":"10.3390/microorganisms12102049","DOIUrl":"https://doi.org/10.3390/microorganisms12102049","url":null,"abstract":"<p><p>To date, the increased awareness of the impact of microbes on human health has promoted scientific interest in microbiome studies for diagnostic and therapeutic purposes, revealing correlations between specific taxa and cancer. In particular, numerous species of <i>Porphyromonas</i> have been associated with several types of tumors. Previously, we studied the urobiome using Next-Generation Sequencing (NGS), and found an increase in <i>Porphyromonas somerae</i> in first morning urine of subjects affected by bladder cancer (BCa). Here, we aimed to confirm the presence of <i>P. somerae</i> in BCa patients by using droplet digital Polymerase Chain Reaction (ddPCR), testing a cohort of 102 male subjects over 50 years. Our findings showed a significant increase in <i>P. somerae</i> in the urine of the BCa group within both ddPCR and NGS, and a correlation between the two methods was observed at a statistical level. Moreover, <i>P. somerae's</i> identification with ddPCR confirmed a significant association between this bacterium and the presence of BCa, highlighting its potential role as a biomarker. This allows us to propose the ddPCR as a suitable method for first-stage BCa screening and follow-up.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota Alterations in Alzheimer's Disease: Relation with Cognitive Impairment and Mediterranean Lifestyle.","authors":"David Mateo, Nerea Carrión, Cristian Cabrera, Luis Heredia, Montse Marquès, Eva Forcadell-Ferreres, Maria Pino, Josep Zaragoza, Alfons Moral, Lluís Cavallé, José M González-de-Echávarri, Paloma Vicens, José L Domingo, Margarita Torrente","doi":"10.3390/microorganisms12102046","DOIUrl":"https://doi.org/10.3390/microorganisms12102046","url":null,"abstract":"<p><p>Recently, an association between dysbiosis of the gut microbiota (GMB) and the development of several diseases, such as Alzheimer's disease (AD), has been proposed. Dysbiosis involves changes in microbial diversity influenced by environmental factors, like diet or lifestyle. In this study, we investigated the role of GMB parameters in Spanish AD patients, assessing the impact of adherence to the Mediterranean lifestyle (ML), as well as to characterize these parameters in relation to neuropsychological, neuropsychiatric, emotional, and functionality parameters. A case-control study was conducted to investigate the association between the composition of the GMB and cognitive, emotional, neuropsychiatric, and functionality status in Spanish AD patients, along with a shotgun metagenomics approach. Richness and alpha-diversity were significantly lower in the AD group compared to the controls. PERMANOVA and ANOSIM tests of Bray-Curtis dissimilarity, Aitchison distance, and Jaccard similarity did not showed significant differences in beta-diversity between the two groups. Moreover, associations between various phyla of the AD group and orientation performance, food consumption, and activities of daily living were identified. Dysbiosis observed in Spanish AD patients is characterized by reductions in richness and alpha-diversity, alongside alterations in GMB composition, which may be linked to adherence to the ML and cognitive and functionality symptoms.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Evidence for Upper Respiratory Infections as a Potential Risk Factor for Meniere's Disease: A Korean National Health Sample Cohort Study.","authors":"Mi Jung Kwon, Ho Suk Kang, Joo-Hee Kim, Ji Hee Kim, Woo Jin Bang, Dae Myoung Yoo, Na-Eun Lee, Kyeong Min Han, Nan Young Kim, Hyo Geun Choi, Min-Jeong Kim, Eun Soo Kim","doi":"10.3390/microorganisms12102047","DOIUrl":"https://doi.org/10.3390/microorganisms12102047","url":null,"abstract":"<p><p>Meniere's disease (MD) is a chronic inner ear disorder characterized by tinnitus, ear fullness, episodic vertigo, and fluctuating hearing loss, which significantly impacts quality of life and poses management challenges. Recent evidence suggests that upper respiratory infections (URIs) may contribute to MD's onset. This study examines the potential link between URIs and MD using data from the Korean National Health Insurance Service-National Sample Cohort (2002-2019). We analyzed 19,721 individuals with MD and 78,884 matched controls, adjusting for demographic factors and comorbidities using propensity score matching. Our results showed that individuals with a URI within one year prior to the index date exhibited a 2.01-fold greater likelihood of developing MD (95% confidence interval [CI] = 1.91-2.11, <i>p</i> < 0.001), while those with URIs within two years demonstrated a 1.54-fold higher probability (95% CI = 1.50-1.59, <i>p</i> < 0.001). Furthermore, we found that even remote URIs occurring up to two years before the index date significantly increased the risk of developing MD, underscoring the need for long-term patient follow-up. Overall, our study suggests that individuals with a history of URI may have an elevated risk of developing MD over multiple time frames, regardless of demographic or health profiles.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil Microorganism Interactions under Biological Fumigations Compared with Chemical Fumigation.","authors":"Hui Li, Huali Man, Jia Han, Xixia Jia, Li Wang, Hongyu Yang, Guiying Shi","doi":"10.3390/microorganisms12102044","DOIUrl":"https://doi.org/10.3390/microorganisms12102044","url":null,"abstract":"<p><strong>Background: </strong>Biological fumigation, a potential alternative to chemical fumigation, shows a wide range of prospective applications. In this study, we carried out biological fumigation experiments to evaluate its effect on alleviating consecutive cropping problems (CRPs) when compared with chemical fumigation.</p><p><strong>Methods: </strong>We designed five treatments, namely, CR (no treatment), LN (chemical fumigation with lime nitrogen), Ta (fumigation with marigold), Ra (fumigation with radish), and Br (fumigation with mustard), for soils for replanting eggplant and measured the crop's growth status, soil bacterial and fungal communities, and soil physicochemical properties.</p><p><strong>Results: </strong>The results showed that the Br and Ra treatments formed similar microbial communities, while the Ta treatment formed unique microbial communities. The genera Olpidiomycota and Rozellomycota could be used as indicator species for the transformation process of soil microbial communities after the Br and Ta treatments, respectively. When compared with the CR and LN treatments, the soil's physicochemical properties were optimized under the Br treatment, and the soil organic matter content increased by 64.26% and 79.22%, respectively. Moreover, under the Br treatment, the soil's biological properties enhanced the bacterial and fungal alpha diversity, and the saprotrophic fungi increased with the depletion of pathotrophic fungi, while some specific probiotic microorganisms (such as Olpidiomycota, Microascales, <i>Bacillus</i>, etc.) were significantly enriched. In contrast, under the Ta treatment, soil nutrient levels decreased and the soil's biological indices deteriorated, whereas the bacterial diversity decreased and the pathogenic fungi increased.</p><p><strong>Conclusions: </strong>Among these three biological fumigation methods, the Br pre-treatment was the best way to alleviate the crop's CRPs and may be a good substitute for chemical fumigation in some situations. However, the Ta treatment also had some risks, such as the loss of land quality and reduced productivity.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Hepatitis C Treatment Monitoring: Is Sustained Virologic Response at 4 Weeks Becoming the New Standard?","authors":"Ivana Milošević, Ana Filipović, Branko Beronja, Nikola Mitrović, Maja Ružić, Jelena Simić, Nataša Knežević, Maria Pete, Nevena Todorović, Nataša Nikolić","doi":"10.3390/microorganisms12102050","DOIUrl":"https://doi.org/10.3390/microorganisms12102050","url":null,"abstract":"<p><p>This study, conducted at two university-based infectious disease clinics, included 216 patients with chronic hepatitis C. The primary objective was to assess the positive and negative predictive values, sensitivity, and specificity of achieving a sustained virological response (SVR) at 4 weeks compared to 12 weeks post-therapy. The results demonstrated a maximum sensitivity of 100% for achieving SVR at 12 weeks after reaching SVR at 4 weeks for all analyzed genotypes, except for genotype 1b treated with EBR/GZR therapy, where the specificity was 75%. Additionally, younger age and less advanced liver fibrosis were identified as independent predictors of achieving a sustained virological response at both 4 and 12 weeks. The significant normalization of various biochemical parameters was observed after treatment, indicating an overall improvement in liver function. This study suggests that shortening the monitoring period to 4 weeks might be effective for younger patients without significant fibrosis, potentially reducing loss to follow-up, which is a critical issue in HCV treatment. These findings align with the \"test and treat\" approach. Further research is needed to confirm these findings and incorporate them into official guidelines, which could simplify and enhance the effectiveness of HCV treatment protocols, aiding global efforts to eliminate HCV as a public health issue by 2030.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory-Simulated Inhibitory Effects of the Floating-Bed Plants on <i>Microcystis aeruginosa</i> and Their Microbial Communities' Responses to Microcystins.","authors":"Shuwen Zhang, Yuanpu Sha, Yuanyuan Tang, Longjie Li, Feihu Wang, Jing Dong, Xuejun Li, Yunni Gao, Xiaofei Gao, Huatao Yuan, Jingxiao Zhang","doi":"10.3390/microorganisms12102035","DOIUrl":"https://doi.org/10.3390/microorganisms12102035","url":null,"abstract":"<p><p>Three common floating bed plants, <i>Eichhornia crassipes</i>, <i>Pistia stratiotes</i>, and <i>Ipomoea aquatica</i>, were selected in the present study to investigate their inhibitory effects on toxic <i>Microcystis aeruginosa</i>. The results showed that all three types of floating-bed plants could considerably inhibit the growth of <i>M. aeruginosa</i> and effectively remove the microcystins (MCs) from water systems, among which, <i>E. crassipes</i> and <i>P. stratiotes</i> were more effective in resisting <i>M. aeruginosa</i>, and the removal rate of the intracellular MCs could be up to 100%. In addition, the roots and leaves of the three plants were enriched with a large number of MCs and demonstrated significant antioxidant responses, as evidenced by the increase in the content of catalase (CAT), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), and malondialdehyde (MDA) in the roots, stems, and leaves of the plants. Furthermore, this study also showed that Proteobacteria, Bacteroidota, Myxococcota, Verrucomicrobiota, and Actinobacteriota dominated the root microorganisms of the three plants. Moreover, a variety of MC-degrading bacteria, including <i>Sphingomonas</i>, <i>Acinetobacter</i>, <i>Novosphingobium</i>, and <i>Pseudomonas</i>, were found at the genus level, which further provides important basic data for the regulation of eutrophic water bodies and the removal of MCs.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}