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Triple-Probiotic-Fermented Goji (Lycium barbarum L.) Ameliorates Metabolic Disorders Associated with Hyperuricemia in Mice. 三益生菌发酵枸杞(枸杞)改善小鼠高尿酸血症相关代谢紊乱。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061367
Lu Ren, Yuechan Li, Shiting Liu, Xiaoke Jia, Hongpeng He, Feiliang Zhong, Fuping Lu, Xuegang Luo
{"title":"Triple-Probiotic-Fermented Goji (<i>Lycium barbarum</i> L.) Ameliorates Metabolic Disorders Associated with Hyperuricemia in Mice.","authors":"Lu Ren, Yuechan Li, Shiting Liu, Xiaoke Jia, Hongpeng He, Feiliang Zhong, Fuping Lu, Xuegang Luo","doi":"10.3390/microorganisms13061367","DOIUrl":"10.3390/microorganisms13061367","url":null,"abstract":"<p><p>Hyperuricemia (HUA) is a metabolic disorder characterized by excessive uric acid (UA) production and impaired excretion. Goji, as a representative medicinal food, holds significant research and development value, while probiotic fermentation technology is finding increasingly widespread applications in the functional food sector. This study developed a novel goji fermented with three probiotic strains (<i>Lactoplantibacillus plantarum</i> CGMCC8198, <i>Lactococcus lactis</i> LTJ28, and <i>Lactocaseibacillus casei</i> YR2-2) and investigated its anti-HUA effects. Optimal fermentation conditions (7.913 material-liquid ratio, 3.92% inoculation, 7.49 h at 37 °C with 1:1:2 strain ratio) yielded a beverage with enhanced flavor profiles (19 aroma compounds) and high viable counts. In HUA cell models, the 15% fermented goji juice significantly reduced UA levels by 56% (<i>p</i> < 0.01). In potassium oxonate-induced HUA mice, the beverage effectively lowered serum UA, xanthine oxidase activity, and renal function markers (blood urea nitrogen and creatinine, <i>p</i> < 0.0001) while improving hepatic parameters (alanine aminotransferase, aspartate Aminotransferase). The goji-fermented juice significantly reduced the expression of renal UA transporters GLUT9 and URAT1 (<i>p</i> < 0.0001) while improving gut microbiota composition, as evidenced by increased beneficial SCFAs (acetic acid, butyric acid, <i>p</i> < 0.0001) and elevated Lactobacillus abundance 2.14-fold. Our findings demonstrate that this triple-probiotic-fermented goji beverage represents an effective dietary strategy for HUA management by simultaneously inhibiting UA production, enhancing excretion, and restoring gut microbiota homeostasis, providing a scientific basis for developing probiotic-based functional foods against HUA.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Analysis of Bacteriome in Hair Follicle Layers of Patients with Female Pattern Androgenic Alopecia. 女性型雄激素性脱发患者毛囊层细菌组的比较分析。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061365
Yujun Park, Seoyeon Kyung, Seyoung Mun, Byung Sun Yu, Kyengeui Yun, Chaeyun Baek, Dong-Geol Lee, Seunghyun Kang, Soon Re Kim, Ju-Hee Kim, Yeji Lee, Byung-Cheol Park, Kyudong Han
{"title":"Comparative Analysis of Bacteriome in Hair Follicle Layers of Patients with Female Pattern Androgenic Alopecia.","authors":"Yujun Park, Seoyeon Kyung, Seyoung Mun, Byung Sun Yu, Kyengeui Yun, Chaeyun Baek, Dong-Geol Lee, Seunghyun Kang, Soon Re Kim, Ju-Hee Kim, Yeji Lee, Byung-Cheol Park, Kyudong Han","doi":"10.3390/microorganisms13061365","DOIUrl":"10.3390/microorganisms13061365","url":null,"abstract":"<p><p>Androgenetic alopecia (AGA) is the most common form of patterned hair loss, exhibiting gender-specific clinical features. Recent studies highlight the importance of the skin microbiome in maintaining skin health, but the relationship between the hair follicle microbiome and hair loss, particularly AGA, remains understudied. Hair follicle layer samples were collected directly from the crown region of female pattern hair loss (FPHL), male pattern hair loss (MPHL), and healthy adult women (control) groups. Microbial DNA was extracted and analyzed using Illumina 16S rRNA V3-V4 gene amplicon sequencing. Alpha-diversity and beta-diversity analyses and taxonomic and functional profiling were conducted through relative abundance, LEfSe, and PICRUSt2 analyses. The alpha-diversity analysis showed a significant decrease in microbial richness in the hair loss groups. Unweighted UniFrac-based beta-diversity analysis revealed significant clustering between the control group and the FPHL group. Taxonomic profiling and LEfSe analysis identified differences in microbial composition and biomarkers. PICRUSt2 analysis further revealed altered pathways related to porphyrin metabolism, fatty acid biosynthesis, and steroid hormone metabolism. Additionally, differences in microbiome composition and potential functions were found between the FPHL and MPHL groups. This study provides comprehensive insights into the hair follicle microbiome, revealing unique microbial patterns and functional alterations associated with FPHL. Understanding these microbiome characteristics may contribute to targeted approaches for addressing AGA. Further research is warranted.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Survey and Genetic Analysis of Ehrlichia canis in Rhipicephalus sanguineus Ticks Infesting Dogs in Northern Taiwan. 台湾北部犬染血头蜱犬埃利希体分子调查及遗传分析。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061372
Chien-Ming Shih, Pei-Yin Ko, Li-Lian Chao
{"title":"Molecular Survey and Genetic Analysis of <i>Ehrlichia canis</i> in <i>Rhipicephalus sanguineus</i> Ticks Infesting Dogs in Northern Taiwan.","authors":"Chien-Ming Shih, Pei-Yin Ko, Li-Lian Chao","doi":"10.3390/microorganisms13061372","DOIUrl":"10.3390/microorganisms13061372","url":null,"abstract":"<p><p><i>Ehrlichia canis</i> is a tick-transmitted zoonotic pathogen in dogs. We conducted a molecular survey for screening of <i>E. canis</i> infection in <i>Rhipicephalus sanguineus</i> ticks infesting dogs and identified its genetic identity in Taiwan. A total of 1195 <i>R. sanguineus</i> ticks were collected and examined for <i>Ehrlichia</i> infection by nested-PCR assay targeting the 16S ribosomal RNA (rRNA) gene. In general, <i>Ehrlichia</i> infection was detected in 1.5% of examined ticks, and was detected in nymph, male and female stages with infection rates of 0.6%, 1.31% and 2.76%, respectively. The highest monthly prevalence was observed in August with an infection rate of 5.91%. Genetic identity was analyzed by comparing the 16S rRNA gene sequences obtained from 11 Taiwan strains and 15 other strains representing five genospecies of <i>Ehrlichia</i> spp., including two outgroups (<i>Anaplasma phagocytophilum</i> and <i>Rickettsia rickettsii</i>). Results revealed that all Taiwan strains were genetically affiliated to the same clade within various <i>E. canis</i> strains documented in GenBank with a high sequence similarity (99.7-100%) and that they can be clearly distinguished from other genospecies of <i>Ehrlichia</i>. This study provides the first evidence of <i>E. canis</i> identified in <i>R. sanguineus</i> ticks and highlights the potential threat for human infections in Taiwan.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification, Comparison, and Profiling of Selected Diarrhoeagenic Pathogens from Diverse Water Sources and Human and Animal Faeces Using Whole-Genome Sequencing. 利用全基因组测序从不同水源和人畜粪便中鉴定、比较和分析选定的腹泻致病菌
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061373
Arinao Murei, Maggy Ndombo Benteke Momba
{"title":"Identification, Comparison, and Profiling of Selected Diarrhoeagenic Pathogens from Diverse Water Sources and Human and Animal Faeces Using Whole-Genome Sequencing.","authors":"Arinao Murei, Maggy Ndombo Benteke Momba","doi":"10.3390/microorganisms13061373","DOIUrl":"10.3390/microorganisms13061373","url":null,"abstract":"<p><p>Consumption of contaminated drinking water is known to cause waterborne diseases such as diarrhoea, dysentery, typhoid, and hepatitis. This study applied whole-genome sequencing (WGS) to detect, identify, compare, and profile diarrhoeagenic pathogens (<i>Vibrio cholerae</i>, Shiga toxin-producing <i>Escherichia coli</i>, and <i>Escherichia coli</i> O157:H7) from 3168 water samples and 135 faecal samples (human and animal). Culture-based methods, MALDI-TOF mass spectrometry, and PCR were employed prior to WGS for identification of pathogens. Culture-based results revealed high presumptive prevalence of STEC (40.2%), <i>V. cholerae</i> (37.1%), and <i>E. coli</i> O157:H7 (22.7%). The MALDI-TOF confirmed 555 isolates with <i>V. cholerae</i> identified as <i>Vibrio albensis</i>. Shiga toxin-producing <i>Escherichia coli</i> (STEC) was more prevalent in wastewater (60%), treated water (54.1%), and groundwater (36.8%). PCR detected 46.4% of virulence genes from the water isolates and 66% of virulence genes from the STEC stool isolates. WGS also revealed STEC (92.9%) as the most prevalent species and found common virulence (e.g., <i>hcp1/tssD1</i> and <i>hlyE</i>) and resistance (e.g., <i>acrA</i> and <i>baeR</i>) genes in all three types of samples. Five resistance and thirteen virulence genes overlapped among treated water and stool isolates. These findings highlight the diarrhoeagenic pathogens' public health risk in water sources and underscore the need for better water quality monitoring and treatment standards.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Competitive Edge: T6SS-Mediated Interference Competition by Vibrionaceae Across Marine Ecological Niches. 竞争优势:t6ss介导的弧菌科海洋生态位干扰竞争
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061370
Perla Jazmin Gonzalez Moreno, Michele K Nishiguchi
{"title":"The Competitive Edge: T6SS-Mediated Interference Competition by <i>Vibrionaceae</i> Across Marine Ecological Niches.","authors":"Perla Jazmin Gonzalez Moreno, Michele K Nishiguchi","doi":"10.3390/microorganisms13061370","DOIUrl":"10.3390/microorganisms13061370","url":null,"abstract":"<p><p>Interference competition, wherein bacteria actively antagonize and damage their microbial neighbors, is a key ecological strategy governing microbial community structure and composition. To gain a competitive edge, bacteria can deploy a diverse array of antimicrobial weapons-ranging from diffusible toxins to contact-mediated systems in order to eliminate their bacterial rivals. Among Gram-negative bacteria, the type VI secretion system (T6SS) has emerged as a potent and sophisticated contact-dependent mechanism that enables the delivery of toxic cargo into neighboring cells, thereby promoting the colonization and dominance of a bacterial taxon within an ecological niche. In this review, we examine the ecological significance of T6SS-mediated interference competition by members of the <i>Vibrionaceae</i> family across a range of marine habitats that include free-living microbial communities and host-associated niches such as coral and squid symbioses. Additionally, we explore the ecological impact of T6SS-mediated competition in modulating biofilm community structure and promoting horizontal gene transfer within those complex microbial populations. Together, these insights underscore the ecological versatility of the T6SS and emphasize its role in driving antagonistic bacterial interactions and shaping microbial community dynamics within marine ecosystems.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial for the Gut Microbiota Section. 肠道微生物组社论。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061364
Pramod K Gopal
{"title":"Editorial for the Gut Microbiota Section.","authors":"Pramod K Gopal","doi":"10.3390/microorganisms13061364","DOIUrl":"10.3390/microorganisms13061364","url":null,"abstract":"<p><p>The gut microbiota-a diverse and dynamic consortium of trillions of microorganisms inhabiting the gastrointestinal tract-plays a pivotal role in host health across species [...].</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypomagnetic Field and Its Effect on the Growth and Survival of Microorganisms. 低磁场及其对微生物生长和存活的影响。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061362
Miroslava Sincak, Kateřina Benediktová, Jana Adámková, Jana Sedlakova-Kadukova
{"title":"Hypomagnetic Field and Its Effect on the Growth and Survival of Microorganisms.","authors":"Miroslava Sincak, Kateřina Benediktová, Jana Adámková, Jana Sedlakova-Kadukova","doi":"10.3390/microorganisms13061362","DOIUrl":"10.3390/microorganisms13061362","url":null,"abstract":"<p><p>As humanity embarks on interplanetary exploration and envisions future colonies beyond Earth, understanding the impact of extreme environments on life becomes paramount. Among these factors, the hypomagnetic field (HMF)-a condition where the protective geomagnetic field is absent-remains poorly understood, especially regarding its effects on (micro)organisms. To our knowledge, this is the first study to examine how short-term exposure to an HMF (24 h to 7 days) affects the growth of three different microorganisms, <i>Saccharomyces cerevisiae</i>, <i>Acidithiobacillus ferrooxidans</i>, and <i>Lactobacillus plantarum</i>, using a specialized hypomagnetic chamber and advanced spectrophotometric analysis. We demonstrate significant growth inhibition in <i>S. cerevisiae</i> (23%) and <i>A. ferrooxidans</i> (68%), with <i>L. plantarum</i> remaining unaffected. This inhibitory effect appears reversible, diminishing as organisms return to normal geomagnetic conditions. These findings reveal that the HMF acts as a temporary environmental stressor, underscoring the need for deeper exploration of its biological effects. Our work sets the stage for further research into how the space environment may shape microbial ecosystems critical to future human endeavors in space.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Characterization of Lactic Acid Bacteria from an Italian Traditional Raw Milk Cheese: Probiotic Properties and Technological Performance of Selected Strains. 意大利传统原料奶酪中乳酸菌的分离与鉴定:益生菌特性及筛选菌株的工艺性能
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061368
Marianna Roselli, Federica Colafranceschi, Valentina Cipriani, Alessandra Valle, Paola Zinno, Barbara Guantario, Emily Schifano, Daniela Uccelletti, Chiara Devirgiliis
{"title":"Isolation and Characterization of Lactic Acid Bacteria from an Italian Traditional Raw Milk Cheese: Probiotic Properties and Technological Performance of Selected Strains.","authors":"Marianna Roselli, Federica Colafranceschi, Valentina Cipriani, Alessandra Valle, Paola Zinno, Barbara Guantario, Emily Schifano, Daniela Uccelletti, Chiara Devirgiliis","doi":"10.3390/microorganisms13061368","DOIUrl":"10.3390/microorganisms13061368","url":null,"abstract":"<p><p>The increasing interest in fermented foods stems from their health benefits, mediated by foodborne microorganisms. This study aimed to characterize the fermentative microbiota of Pecorino di Picinisco, a traditional Italian cheese made from ovine raw milk, and to evaluate the probiotic and technological potential of selected lactic acid bacteria strains. Three strains representative of the different species found (<i>Lactococcus lactis</i>, <i>Lactiplantibacillus plantarum</i> and <i>Latilactobacillus curvatus</i>) were chosen and analyzed. All three strains were able to adhere to human intestinal Caco-2 cells, were resistant to simulated in vitro digestion and significantly prolonged the lifespan of <i>Caenorhabditis elegans</i>, used as a simplified <i>in vivo</i> model, with respect to the commercial probiotic strain <i>Lacticaseibacillus rhamnosus</i> GG. The <i>L. plantarum</i> Pic37.4 strain was particularly promising; therefore, its cell-free supernatant was employed to evaluate the antimicrobial activity against indicator strains of foodborne and intestinal pathogens or spoilage bacteria. The results demonstrated the effectiveness of the supernatant against all strains tested, with the strongest effect on the intestinal pathogen enterotoxigenic <i>Escherichia coli</i> K88. In addition, the inhibitory effect on pathogen adhesion to intestinal mucosa was investigated on Caco-2 cells, resulting in a significant reduction in adhesion mediated by the <i>L. plantarum</i> Pic37.4 supernatant. The antimicrobial properties of the <i>L. plantarum</i> strain were confirmed <i>in vivo</i> in <i>C. elegans</i>. These promising results lay the ground for further investigations aimed at substantiating the probiotic and technological potential of the <i>L. plantarum</i> Pic37.4 investigated in this work.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Impact of Jujube Witches' Broom Phytoplasma on the Community Structure of Endophytes in Jujube. 枣树巫帚菌原体对枣树内生菌群落结构的影响。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061371
Nian Wang, Mengli Wang, Ziming Jiang, Wenzhe Zhang, Ziyang You, Xueru Zhao, Jia Yao, Chenrui Gong, Assunta Bertaccini, Jidong Li
{"title":"The Impact of Jujube Witches' Broom Phytoplasma on the Community Structure of Endophytes in Jujube.","authors":"Nian Wang, Mengli Wang, Ziming Jiang, Wenzhe Zhang, Ziyang You, Xueru Zhao, Jia Yao, Chenrui Gong, Assunta Bertaccini, Jidong Li","doi":"10.3390/microorganisms13061371","DOIUrl":"10.3390/microorganisms13061371","url":null,"abstract":"<p><p>Evidence from an increasing number of studies indicates that plant endophytic microorganisms play a significant role during biotic and abiotic stress resistance. To date, however, only a handful of studies on endophytes in response to the presence of phytoplasmas have been conducted. The production of jujube (<i>Ziziphus jujuba</i>) is threatened by jujube witches' broom (JWB) disease, which is associated with the presence of the JWB phytoplasma '<i>Candidatus</i> Phytoplasma ziziphi'. To investigate the impact of jujube witches' broom phytoplasma on the endophyte populations in jujube, high-throughput sequencing was performed in healthy and JWB-infected orchard jujube trees and <i>in vitro</i> jujube shoots. The results showed that the presence of JWB phytoplasma in jujube altered the abundance, diversity, and community structure of endophytic bacteria and fungi. In the branches and the roots, the presence of JWB phytoplasma was associated with an increase in the richness of the endophytic communities and a decrease in their diversity, with the phyla Proteobacteria, Firmicutes, and Bacteroidota and the genus '<i>Ca</i>. Phytoplasma' becoming the most abundant. The presence of phytoplasmas was also associated with the remodeling of the endophytic microorganisms' interaction network, shifting to a simpler biodiversity state. These results demonstrate the response of the jujube endophytic community to the presence of JWB phytoplasmas and shed light on the possible antagonistic agents that could be further evaluated for JWB disease biocontrol.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening and Isolation of Bacterial Strains Able to Degrade Trimethylamine. 降解三甲胺细菌的筛选与分离。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061369
Sebastião V T F de Almeida, Kilian Neves, Carla C C R de Carvalho
{"title":"Screening and Isolation of Bacterial Strains Able to Degrade Trimethylamine.","authors":"Sebastião V T F de Almeida, Kilian Neves, Carla C C R de Carvalho","doi":"10.3390/microorganisms13061369","DOIUrl":"10.3390/microorganisms13061369","url":null,"abstract":"<p><p>Methylamines are present in numerous organisms and microorganisms capable of de novo trimethylamine (TMA) production are widely distributed, including microalgae. However, such compounds may hamper the application of microalgae biomass in commercially interesting products, such as food and feed products, due to the strong fishy smell. In the present study, several bacteria able to degrade TMA were isolated. Among them, a <i>Staphylococcus saprophyticus</i> strain was found particularly suitable to degrade TMA. After finding the best culture conditions, a bioprocess system was developed allowing the degradation of TMA from microalgae in a reactor by <i>S. saprophyticus</i> cells present in a second reactor without direct contact with media from both reactors. The system was found to be limited by TMA transfer through the gas phase, with the cells being able to degrade all available TMA.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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