Microorganisms最新文献_第8页

Exploring the Gut Microbiota-Retina Axis: Implications for Health and Disease. 探索肠道微生物群-视网膜轴：对健康和疾病的影响。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-10 DOI: 10.3390/microorganisms13051101

Nicola Schiavone, Giulia Isoldi, Sara Calcagno, Elisabetta Rovida, Emiliano Antiga, Carolina Vieira De Almeida, Matteo Lulli

{"title":"Exploring the Gut Microbiota-Retina Axis: Implications for Health and Disease.","authors":"Nicola Schiavone, Giulia Isoldi, Sara Calcagno, Elisabetta Rovida, Emiliano Antiga, Carolina Vieira De Almeida, Matteo Lulli","doi":"10.3390/microorganisms13051101","DOIUrl":"10.3390/microorganisms13051101","url":null,"abstract":"The gut microbiota represents a rich and adaptive microbial network inhabiting the gastrointestinal tract, performing key functions in nutrient processing, immune response modulation, intestinal wall protection, and microbial defense. Its composition remains highly personalized and responsive to external influences, including lifestyle patterns, physical activity, body composition, and nutritional intake. The interactions of the gut microbiota with bodily systems are conventionally interpreted as broad systemic impacts on organ balance. Yet, emerging research-exemplified by the gut microbiota-brain axis-suggests the potential existence of more targeted and direct communication mechanisms. Dysbiosis, characterized by microbial ecosystem disturbance, generates multiple metabolic compounds capable of entering systemic circulation and reaching distant tissues, notably including ocular structures. This microbial imbalance has been associated with both systemic and localized conditions linked to eye disorders. Accumulating scientific evidence now supports the concept of a gut-retina axis, underscoring the significant role of microbiota disruption in generating various retinal pathologies. This review comprehensively investigates gut microbiota composition, functional dynamics, and dysbiosis-induced alterations, with specific focus on retinal interactions in age-related macular degeneration, diabetic retinopathy, glaucoma, and retinal artery occlusion. Moreover, the review explores microbiota-targeted therapeutic strategies, including precision nutritional interventions and microbial transplantation, as potential modulators of retinal disease progression.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring the Occurrence of Aspergillus in the Air of Intensive Care Units. 重症监护病房空气中曲霉的监测。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-09 DOI: 10.3390/microorganisms13051099

Anna Inglot, Agnieszka Gniadek, Zuzanna Tokarz, Wirginia Krzyściak, Monika Papież, Paweł Krzyściak

{"title":"Monitoring the Occurrence of Aspergillus in the Air of Intensive Care Units.","authors":"Anna Inglot, Agnieszka Gniadek, Zuzanna Tokarz, Wirginia Krzyściak, Monika Papież, Paweł Krzyściak","doi":"10.3390/microorganisms13051099","DOIUrl":"10.3390/microorganisms13051099","url":null,"abstract":"Poor air quality within hospitals can contribute to a range of health issues, collectively known as sick hospital syndrome, encompassing respiratory, skin, and nonspecific symptoms. Aspergillus poses a significant risk of severe respiratory infections, particularly within intensive care unit (ICU) patients often with compromised immune systems. This study was conducted in the intensive care units of three selected hospitals in Cracow, Poland. Air samples were obtained using the single-stage impactor MAS-100 NT Ex (Merck). The air samples were taken from each ward, seasonally, four times a year. Sampling of intensive care units' air for Aspergillus revealed the significant diversity in fungal concentration and unveiled seasonal fluctuations in culturable fungi levels. The highest concentration of Aspergillus species complexes was detected during autumn, while the lowest was in spring. The disturbing occurrence of Aspergillus in hospitals' air emphasizes the importance of monitoring fungal air bioburden and assessing air control efficiency and highlights the urgent need to develop and validate microbiological standards for fungal air contamination in hospitals.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling Pediatric Group A Streptococcus Meningitis: Lessons from Two Case Reports and a Systematic Review. 儿童A组链球菌性脑膜炎：来自两个病例报告和系统回顾的教训。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-09 DOI: 10.3390/microorganisms13051100

Lavinia Di Meglio, Maia De Luca, Laura Cursi, Lorenza Romani, Mara Pisani, Anna Maria Musolino, Stefania Mercadante, Venere Cortazzo, Gianluca Vrenna, Paola Bernaschi, Roberto Bianchi, Laura Lancella

{"title":"Unraveling Pediatric Group A Streptococcus Meningitis: Lessons from Two Case Reports and a Systematic Review.","authors":"Lavinia Di Meglio, Maia De Luca, Laura Cursi, Lorenza Romani, Mara Pisani, Anna Maria Musolino, Stefania Mercadante, Venere Cortazzo, Gianluca Vrenna, Paola Bernaschi, Roberto Bianchi, Laura Lancella","doi":"10.3390/microorganisms13051100","DOIUrl":"10.3390/microorganisms13051100","url":null,"abstract":"Streptococcus pyogenes meningitis is a rare invasive disease, accounting for less than 2% of bacterial meningitis. We presented two case reports and conducted a systematic review using PUBMED, covering the database from its inception up to 31 December 2024, of pediatric cases of Streptococcus pyogenes meningitis. Only case reports and case series were included. Differences in clinical and laboratory parameters were compared between uneventful course and complicated admissions. A total of 57 cases were included. The median age at diagnosis was 4 years. A primary infection focus outside the brain was identified in 61.39% of cases. S. pyogenes was identified from cerebrospinal fluid in 66.66% of cases and from blood in 15.79%. Septic shock occurred in 24.56% of cases, and 36.84% had brain anatomical anomalies. All patients received broad-spectrum empiric antibiotics, while protein-synthesis inhibitors were administered in 26.31% of cases. A total of 17% of patients died, and 28.07% experienced sequelae. The identification of S. pyogenes from blood and a Phoenix Sepsis Score ≥ 2 were significantly associated with a complicated clinical course. Our findings may offer useful insights for the clinical management of Streptococcus pyogenes meningitis.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Revegetation Alters Habitat Conditions, Bacterial Components, and Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Communities in Aged PAH-Polluted Soils. 自然植被恢复改变了多环芳烃污染土壤的生境条件、细菌组成和多环芳烃降解群落

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-09 DOI: 10.3390/microorganisms13051098

Jinrong Huang, Heng Liang, Lilong Huang, Qi Li, Lei Ji, Yingna Xing, Chang Zhou, Jianing Wang, Xiaowen Fu

{"title":"Natural Revegetation Alters Habitat Conditions, Bacterial Components, and Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Communities in Aged PAH-Polluted Soils.","authors":"Jinrong Huang, Heng Liang, Lilong Huang, Qi Li, Lei Ji, Yingna Xing, Chang Zhou, Jianing Wang, Xiaowen Fu","doi":"10.3390/microorganisms13051098","DOIUrl":"10.3390/microorganisms13051098","url":null,"abstract":"The vegetation restoration of contaminated sites plays a critical role in ensuring the sustained stability and functional integrity of natural ecosystems. However, during the natural revegetation process, the variations in habitat conditions, bacterial community structure, and metabolic functions in aged, polluted soil are still unclear. In the present study, we investigated aged, polycyclic aromatic hydrocarbon (PAH)-polluted soils at closed, abandoned oil well sites from the Yellow River Delta. Using gene amplification and real-time qPCR methods, the abundance, taxonomy, and diversity characteristics of indigenous bacterial communities and functional bacteria carrying C12O genes in both vegetated soils and bare soils were investigated. The results show that natural revegetation significantly changes the physicochemical parameters, PAH content, and bacterial community structure of aged, PAH-polluted soils. When comparing the abundance and components of PAH-degrading bacterial communities in vegetated and bare soils, the PAH-degrading potential was revealed to be stimulated by vegetation communities. Through correlation analysis, dual stress from soil salinity and PAH contamination in bacterial communities was revealed to be mediated through alterations in the soil's physicochemical properties by local vegetation. The network analysis revealed that bacterial communities in vegetated soils have higher network connectivity. These results elucidate the alterations in habitat conditions, bacterial components, and PAH-degrading communities following vegetation restoration, providing critical insights for optimizing ecological rehabilitation strategies in salinized and contaminated ecosystems.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brevibacterium Species Infections in Humans-A Narrative Review. 人类短杆菌感染研究述评

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-09 DOI: 10.3390/microorganisms13051097

Takis Panayiotou, Anastasia Vasilopoulou, Stella Baliou, Andreas G Tsantes, Petros Ioannou

{"title":"Brevibacterium Species Infections in Humans-A Narrative Review.","authors":"Takis Panayiotou, Anastasia Vasilopoulou, Stella Baliou, Andreas G Tsantes, Petros Ioannou","doi":"10.3390/microorganisms13051097","DOIUrl":"10.3390/microorganisms13051097","url":null,"abstract":"Brevibacterium species are Gram-positive, non-sporulating, coryneform, aerobic rods that are catalase positive and exhibit a distinctive transition from diptheroid to coccoid morphology during culture. Infections by these species are seldom identified. Objective: This narrative review aims to present all the reported cases of Brevibacterium spp. infections in humans, focusing on data about epidemiology, antimicrobial resistance, antimicrobial treatment, and mortality. A narrative review based on a literature search of PubMed/MedLine and Scopus databases was performed. In total, 41 studies providing data on 42 patients with Brevibacterium spp. infections were included in the present analysis. The median age was 48 years, while 57.5% were male. The presence of a central venous catheter and malignancy, and end-stage renal disease on peritoneal dialysis were the main predisposing factors. Bacteremia was the most common type of infection, with peritoneal dialysis-associated infections being the second most common. B. casei was the most commonly identified species. Microbial identification required the use of advanced molecular techniques, such as 16s rRNA sequencing or matrix-assisted laser desorption/ionization time of flight mass spectrometry in most cases. Brevibacterium spp. was highly resistant to the combination of trimethoprim with sulfamethoxazole, clindamycin, and common beta-lactams. The most commonly used antimicrobials were vancomycin and aminoglycosides. The mortality was about 10%. Clinicians and laboratory personnel should consider this pathogen in the differential diagnosis in patients with malignancy or peritoneal dialysis-associated peritonitis. Vancomycin should be used for empirical treatment and while antimicrobial susceptibility testing results are pending.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico Characterization of Resistance and Virulence Genes in Aeromonas jandaei Strains Isolated from Oreochromis niloticus in Brazil. 巴西nilochromis jandaei气单胞菌抗性和毒力基因的硅片分析。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051094

Marcela Laryssa Oliveira Duarte, Diego Lucas Neres Rodrigues, Gabryel Bernardo Vieira de Lima, Juan Carlos Ariute, Gisele Veneroni Gouveia, João José de Simoni Gouveia, Vasco Azevedo, Bertram Brenig, Eric Guédon, Guilherme Campos Tavares, Mateus Matiuzzi da Costa, Ulisses de Pádua Pereira, Flávia Figueira Aburjaile

{"title":"In Silico Characterization of Resistance and Virulence Genes in Aeromonas jandaei Strains Isolated from Oreochromis niloticus in Brazil.","authors":"Marcela Laryssa Oliveira Duarte, Diego Lucas Neres Rodrigues, Gabryel Bernardo Vieira de Lima, Juan Carlos Ariute, Gisele Veneroni Gouveia, João José de Simoni Gouveia, Vasco Azevedo, Bertram Brenig, Eric Guédon, Guilherme Campos Tavares, Mateus Matiuzzi da Costa, Ulisses de Pádua Pereira, Flávia Figueira Aburjaile","doi":"10.3390/microorganisms13051094","DOIUrl":"10.3390/microorganisms13051094","url":null,"abstract":"Understanding the genetic characteristics of Aeromonas jandaei in Brazilian aquaculture is crucial for developing effective control strategies against this fish pathogen. The present study conducted a genomic analysis of Brazilian A. jandaei strains with the objective of investigating their virulence potential and resistance profiles. Four Brazilian isolates were subjected to sequencing, and comparative genomic analyses were conducted in conjunction with 48 publicly available A. jandaei genomes. The methods employed included quality assessment, de novo assembly, annotation, and analyses of antimicrobial resistance and virulence factors. The results demonstrated the presence of fluoroquinolone resistance genes within the core genome. Notably, these antibiotics are not authorized for use in aquaculture in Brazil, suggesting that their resistance determinants may originate from other selective pressures or horizontal gene transfer unrelated to aquaculture practices. The analysis identified significant virulence mechanisms, including T2SS, T3SS, and notably T6SS (vgrG3 gene), which was more prevalent in Brazilian isolates. Additionally, genes associated with motility, adhesion, and heavy metal resistance were identified. These findings highlight the enhanced adaptability of Brazilian A. jandaei strains and raise concerns about antimicrobial resistance in aquaculture, emphasizing the need for improved regulatory oversight and control strategies.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Vineyard Microbiome: How Climate and the Main Edaphic Factors Shape Microbial Communities. 葡萄园微生物群落：气候和主要土壤因子如何塑造微生物群落。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051092

Vanessa Silva, Isabel Brito, Ana Alexandre

引用次数: 0

Reducing Functional Domain of Histatin 5 Improves Antifungal Activity and Prevents Proteolytic Degradation. 减少组蛋白5的功能域提高抗真菌活性和防止蛋白水解降解。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051091

Carolina R Zambom, Gabriel Bernardes, Fauller Henrique da Fonseca, Gabriela Vieira Silva Zolin, Mariana de Melo Faceto Portella, Lina Maria Marin, Edson Crusca, Ed S Krol, Walter L Siqueira, Saulo Santesso Garrido

{"title":"Reducing Functional Domain of Histatin 5 Improves Antifungal Activity and Prevents Proteolytic Degradation.","authors":"Carolina R Zambom, Gabriel Bernardes, Fauller Henrique da Fonseca, Gabriela Vieira Silva Zolin, Mariana de Melo Faceto Portella, Lina Maria Marin, Edson Crusca, Ed S Krol, Walter L Siqueira, Saulo Santesso Garrido","doi":"10.3390/microorganisms13051091","DOIUrl":"10.3390/microorganisms13051091","url":null,"abstract":"Histatin 5 (Hst5) is an antifungal peptide (AFP) naturally produced by parotid glands with strong activity against Candida albicans. One of its mechanisms of action is the generation of reactive oxygen species (ROS) inside the C. albicans cells. Despite being an important peptide for the human innate immune response, its activity is reduced or inactivated by proteolytic degradation caused by salivary enzymes. To overcome this barrier, we used solid phase peptide synthesis (SPPS) to modify the Hst5 amino acid sequence improving its antifungal action and minimizing its degradation. We synthesized five peptides, three of which were based on the Hst5 functional domain. We determined that the smallest peptides (8WH5, 7WH5 and 6WH5) demonstrated the greatest antifungal action against C. albicans, including one fluconazole-resistant strain. Besides that, cationic-PAGE and HPLC assays showed that the degradation in saliva was slower for the smaller peptides than for 0WHst5 and WP113. Furthermore, 8WH5, 7WH5 and 6WH5 were found in the samples even after 8 h in whole saliva, while 0WHst5 and WP113 completely disappear after 1.5 h. Finally, we found that the smaller peptides were less fragmented than the 0WHst5 and WP113, so they were the smallest fragments of Hst5 to preserve its antifungal action with reduced degradation in whole saliva. Thus, they can be considered promising molecules for the treatment of C. albicans in the oral cavity.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porcine Epidemic Diarrhea Virus Is Inhibited by GS-441524 During an In Vitro Infection. GS-441524对猪流行性腹泻病毒体外感染的抑制作用

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051089

Shijuan Dong, Rujing Sun, Bingqing Chen, Fusheng Si, Chunhua Li, Daojing Zhang, Ruisong Yu, Huili Liu

{"title":"Porcine Epidemic Diarrhea Virus Is Inhibited by GS-441524 During an In Vitro Infection.","authors":"Shijuan Dong, Rujing Sun, Bingqing Chen, Fusheng Si, Chunhua Li, Daojing Zhang, Ruisong Yu, Huili Liu","doi":"10.3390/microorganisms13051089","DOIUrl":"10.3390/microorganisms13051089","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV), the etiology of porcine epidemic diarrhea (PED), continues to impose severe economic burdens on pig farms in China. The continuous emergence of new variant strains makes it difficult for vaccinated sows to provide protective immunity to piglets. Hence, there is an urgent need for efficacious therapeutic drugs in clinical practice. In the present study, the antiviral activity of GS-441524, a nucleoside analogue, against PEDV was evaluated. It can efficiently inhibit the proliferation of trypsin-dependent and trypsin-independent PEDVs in a dose-dependent manner, exhibiting greater efficacy against the trypsin-independent strain. GS-441524 can inhibit trypsin-independent PEDV proliferation in Vero cells with EC50 and CC50 values of 2.6 μM and 104.4 μM, respectively. As expected, GS-441524 exerts its inhibitory effect during the replication phase of the four stages of the PEDV proliferation cycle. Even at a high viral infection dose of MOI 0.5 or added 6 h post-viral infection, 20 μM GS-441524 can still effectively inhibit PEDV proliferation. These findings emphasize the potent antiviral activity of GS-441524 against PEDV, and its therapeutic efficacy on PEDV-infected piglets warrants further investigation.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Lectin AtTL-2 Obtained from Acropora aff. tenuis Induced Stimualation of Phagocytosis of Symbiodiniaceae. 鹿茸凝集素AtTL-2诱导刺激共生菌科植物的吞噬作用。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051095

Mitsuru Jimbo, Nami Kuniya, Yuna Fujimaki, Daiki Yoshikawa, Naoki Kamiya, Haruna Amano, Ko Yasumoto, Ikuko Yuyama, Go Suzuki, Saki Harii

{"title":"A Lectin AtTL-2 Obtained from Acropora aff. tenuis Induced Stimualation of Phagocytosis of Symbiodiniaceae.","authors":"Mitsuru Jimbo, Nami Kuniya, Yuna Fujimaki, Daiki Yoshikawa, Naoki Kamiya, Haruna Amano, Ko Yasumoto, Ikuko Yuyama, Go Suzuki, Saki Harii","doi":"10.3390/microorganisms13051095","DOIUrl":"10.3390/microorganisms13051095","url":null,"abstract":"The coral Acropora aff. tenuis selectively acquired various zooxanthella (Symbiodiniaceae) strains, and one of the selective factors was lectins. The A. aff. tenuis lectin AtTL-2 was identified as a factor for Symbiodiniaceae acquisition by the coral, but the mechanism is not fully known. The acquisition process involves three steps: chemotaxis, entry into the coral, and phagocytosis. In this study, we examined the function of AtTL-2 in more detail. Immunohistochemistry analysis was performed to examine the distribution of AtTL-2. The effect of AtTL-2 on the number of Symbiodiniaceae acquired was measured in A. aff. tenuis juvenile polyps with and without AtTL-2 siRNA treatment. The effect of AtTL-2 fixation was examined by monitoring the acquisition of AtTL-2-fixed beads by A. aff. tenuis. AtTL-2 was distributed in nematocysts, spirocysts, and around Symbiodiniaceae. AtTL-2 siRNA inhibited the acquisition of Symbiodiniaceae by juvenile polyps. Fixation of AtTL-2 promoted bead acquisition by juvenile polyps more than fixation of bovine serum albumin (BSA). Moreover, more AtTL-2-fixed beads were bound to the Symbiodiniaceae-enclosed cells than BSA-fixed beads. AtTL-2 is released from spirocysts and binds to Symbiodiniaceae. AtTL-2 then promotes the phagocytosis of Symbiodiniaceae by gastrodermal cells of A. aff. tenuis.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12114434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0