Microorganisms最新文献

{"title":"Correction: Baghdadi et al. Contribution of Manure-Spreading Operations to Bioaerosols and Antibiotic Resistance Genes' Emission. <i>Microorganisms</i> 2023, <i>11</i>, 1797.","authors":"Mahsa Baghdadi, Patrick Brassard, Stéphane Godbout, Valérie Létourneau, Nathalie Turgeon, Florent Rossi, Émie Lachance, Marc Veillette, Marie-Lou Gaucher, Caroline Duchaine","doi":"10.3390/microorganisms13030625","DOIUrl":"10.3390/microorganisms13030625","url":null,"abstract":"<p><p>In the original publication [...].</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do Hospitalized Adult Patients with Acute Pharyngotonsillitis Need Empiric Antibiotics? The Impact on Antimicrobial Stewardship.","authors":"Chih-Wei Liang, Mei-Cheng Hsiao, Shin-Huei Kuo, Shang-Yi Lin, Nai-Hwa Shih, Min-Han Hsieh, Tun-Chieh Chen, Po-Liang Lu","doi":"10.3390/microorganisms13030628","DOIUrl":"10.3390/microorganisms13030628","url":null,"abstract":"<p><p>Acute pharyngotonsillitis is a common reason to visit primary care providers. Group A Streptococcal (GAS) pharyngitis is the most common bacterial infection which needs antibiotic treatment. GAS accounts for only 10-15% of adult acute pharyngitis cases. The overuse of antibiotics for viral pharyngotonsillitis is common and may lead to inappropriate antimicrobial stewardship and the emergence of bacterial resistance. However, the etiology of acute pharyngotonsillitis for hospitalized adult patients is rarely studied. So, we reported the 10-year surveillance data of hospitalized adult patients with acute pharyngotonsillitis in a regional hospital in Taiwan. Every consecutive adult patient admitted with acute pharyngotonsillitis in 2011-2021 was recruited for a complete etiology study. The etiology of acute pharyngotonsillitis was identified in 117 patients. Overall, 42 herpes simplex virus cases, 26 adenovirus cases, 16 acute human immunodeficiency virus cases, 12 influenza cases, three parainfluenza cases, six Epstein-Barr virus cases, one cytomegalovirus case, four enterovirus cases, one varicella-zoster virus case, four <i>Mycoplasma pneumoniae</i> cases, one <i>Chlamydophila pneumoniae</i> case, and only one GAS case were identified. The average of the points for the Modified Centor Criteria was 1.38 (55% of patients with 0-1 points and 45% with 2-3 points). However, 88.9%of patients received antibiotics at the emergency department, and 76.9%also received antibiotics while hospitalized. Only a few patients required antibiotic treatment, while the majority of patients with viral illness needed only symptomatic treatment. However, distinguishing viral etiology from GAS pharyngitis is challenging even in the presence of tonsil exudates, high C-reactive protein, and leukocytosis. A diagnostic algorithm and the application of the Modified Centor Criteria should be considered for hospitalized adults with acute pharyngotonsillitis to improve antimicrobial stewardship.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bifidobacterium adolescentis PRL2019</i> in Pediatric Irritable Bowel Syndrome: A Multicentric, Randomized, Double-Blind, Placebo-Controlled Trial.","authors":"Valentina Giorgio, Giovanna Quatrale, Maurizio Mennini, Marisa Piccirillo, Silvia Furio, Giuseppe Stella, Alessandro Ferretti, Pasquale Parisi, Melania Evangelisti, Enrico Felici, Paolo Quitadamo, Giovanni Di Nardo","doi":"10.3390/microorganisms13030627","DOIUrl":"10.3390/microorganisms13030627","url":null,"abstract":"<p><p>The gut microbiota plays a pivotal role in gastrointestinal inflammation and immune response since changes in microbiota may result in abnormal neurotransmitter expression, inducing changes in gastrointestinal sensory-motor function and leading to symptom onset in irritable bowel syndrome (<i>IBS</i>) patients. The <i>Bifidobacterium adolescentis</i> species has a documented immunomodulatory effect through its ability to produce γ-aminobutyric acid (<i>GABA</i>), the primary inhibitory neurotransmitter in the mammalian central nervous system, which is reduced in <i>IBS</i> patients. This is a multicentric, randomized, double-blind, placebo-controlled, parallel-arm trial aimed at evaluating the effectiveness of <i>Bifidobacterium adolescentis PRL2019</i> in children with <i>IBS. IBS</i> children diagnosed according to Rome IV criteria were enrolled and randomized into two groups to receive one stick containing 20 × 10⁹ colony-forming unit of <i>Bifidobacterium adolescentis PRL2019</i> (Gabapral, Pontenure, Italy) or an equivalent placebo once a day, in a 1:1 ratio, for 12 weeks. Clinical evaluation of symptoms was performed every four weeks using validated scores. Bowel habit characteristics were assessed using the Bristol Stool Chart (<i>BSC</i>). Seventy-two subjects (mean age 12.2 ± 1.8 years, 30 males) were enrolled and randomized into two groups, each of thirty-six patients. No significant differences were observed between the two groups regarding demographic characteristics, distribution of <i>IBS</i> subtypes, or baseline measures of <i>IBS</i> severity and <i>BSC</i>. The proportion of patients achieving complete remission was significantly higher in the <i>BA</i> Group (19/36; 52.8%) than in the Placebo Group (7/36; 19.4%, <i>p</i> = 0.003, odds ratio [OR] 0.216, 95% confidence interval [CI] 0.075-0.619). Both groups obtained a reduction in Total <i>IBS</i> Symptom Severity Scale (<i>IBS SSS</i>), Pain Intensity Score (<i>PIS</i>), Pain Frequency Score (<i>PFS</i>), and Life Interference Score (<i>LIS</i>) from T0 to T12. However, upon intergroup comparison, only in the <i>BA</i> group did the <i>IBS-SSS</i> (<i>p</i> = 0.001), <i>PIS</i> (<i>p</i> = 0.001), <i>LIS</i> (<i>p</i> = 0.015), and <i>PFS</i> (<i>p</i> = 0.005) significantly improve between T0 and T12. <i>BSC</i> showed a greater representation of normal stools (type 3-4) at the end of treatment in the <i>BA</i> group compared with baseline (25% vs. 58.3%, <i>p</i> = 0.004), especially in patients who presented an <i>IBS</i>-constipation subtype at T0 (44.5% vs. 19.4%, <i>p</i> = 0.02). In our study, <i>Bifidobacterium adolescentis PRL2019</i> reduces the severity and frequency of symptoms in children with <i>IBS</i>, positively affecting bowel habits in children with the <i>IBS</i>-constipation subtype.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiology and Risk Factors of Recurrent Urinary Tract Infections in Women in a Multidisciplinary Hospital in Romania.","authors":"Corina-Ioana Anton, Ion Ștefan, Mădălina Zamfir, Constantin Florin Ghiațău, Cristian Sorin Sima, Coralia Luciana Osman, Teodora Alexia Ștefan, Adrian Streinu-Cercel","doi":"10.3390/microorganisms13030626","DOIUrl":"10.3390/microorganisms13030626","url":null,"abstract":"<p><strong>Background: </strong>Urinary tract infections (UTIs) are among the most prevalent bacterial infections affecting millions of people worldwide each year. They are characterized by the presence of pathogenic microorganisms in the urinary system, leading to symptoms such as frequent urination, burning sensations during urination, and lower abdominal pain. While UTIs can affect individuals of all ages and genders, they are particularly common in women due to anatomical factors. A significant concern regarding UTIs is their tendency to recur, with some patients experiencing multiple episodes within a year.</p><p><strong>Methods: </strong>This study included 674 female patients that were admitted into \"Dr. Carol Davila\" Central Military Emergency University Hospital in Bucharest over a period of 3 years.</p><p><strong>Results: </strong>Of the 674 women with urinary tract infection, 435 (79.3%) had more than one positive culture, and 239 (35.4%) experienced at least one recurrent urinary tract infection 6-12 months after the initial diagnosis. The mean age of women with urinary tract infection was 63 (±15.61 years). Recurrent urinary tract infections were most prevalent in those aged 65-80 years (52%). <i>Escherichia coli</i> was detected in 71% of the positive cultures.</p><p><strong>Conclusions: </strong>The high prevalence of UTIs among women is a significant public health concern that warrants attention. Women are particularly susceptible to UTIs due to various anatomical and physiological factors. This increased vulnerability underscores the importance of understanding the current epidemiological landscape of UTIs to develop effective preventive strategies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of Soil Microbial Communities Associated with Phosphorus Transformation to Land-Use Alternations in a Meadow Grassland, Northeast China.","authors":"Li Yu, Ying Zhang, Zhenbo Cui, Chengyou Cao","doi":"10.3390/microorganisms13030624","DOIUrl":"10.3390/microorganisms13030624","url":null,"abstract":"<p><p>Land-use changes in meadow grasslands in semi-arid areas usually significantly affect soil environment and microbiota. However, studies on the response of soil P-cycle-related microbial communities to land-use conversions are still limited. In this study, a series of land-use types including upland field, paddy field, poplar plantation, and their adjacent natural meadow grassland in the Horqin Sandy Land of Northeast China were selected, and the diversities and structures of soil microbial communities involved in organic P mineralization (<i>phoD</i>-harboring community) and inorganic phosphate solubilization (<i>gcd</i>-harboring community) were investigated by the high-throughput sequencing technique. Land-use type had significant influences on soil physicochemical properties, enzymatic activities, and P conversion rates, thereby altering the structures of soil <i>gcd</i> and <i>phoD</i> communities. Soil <i>phoD</i> microbes are more abundant and have more contributions to available P than <i>gcd</i> microbes. The responses of <i>gcd</i> or <i>phoD</i> communities to land-use type were characterized as the quantitative shift in the relative abundance of dominant taxa; however, the basic compositions of the two communities were slightly affected. Soil pH, EC, and nutrient contents (including organic matter and total and available N, P, and K) all significantly affected soil <i>gcd</i> and <i>phoD</i> microbial communities. The abundance of <i>phoD</i> and <i>gcd</i> genes varied with land-use type and could be used as indicators for estimating the bioavailability of soil P.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Culturable Skin Microbiome of Horses from Southern Germany.","authors":"Mahdis Matinpour, Nadine Zettner, Kristin Neumann, Lisa Bäumer, Andreas Burkovski","doi":"10.3390/microorganisms13030623","DOIUrl":"10.3390/microorganisms13030623","url":null,"abstract":"<p><p>Horses have close interactions with humans and are important as working animals and livestock. In contrast to smaller companion animals like cats and dogs, there is only little information available about their skin microbiome. The objective of this study was to identify and characterize the culturable cutaneous microbiome of healthy horses. Samples were taken from 14 horses from Southern Germany which were randomly enrolled in this study. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a method to detect the culturable microorganisms of horse skin. The most abundant culturable species of horse skin identified in this study include <i>Bacillus cereus</i>, <i>Bacillus pumilus</i>, <i>Carnobacterium inhibens</i>, <i>Exiguobacterium sibiricum</i>, <i>Macrococcus carouselicus</i>, <i>Macrococcus goetzii</i> and <i>Staphylococcus equorum</i>. Analyses of the bacteria across different body regions indicated the specific preferences of species for certain skin areas. In addition, our data hinted to an influence of the age of the horses tested and an influence between the four stables studied.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Potential Link Between Oral Microbiota and Female Reproductive Health.","authors":"Justyna Marcickiewicz, Małgorzata Jamka, Jarosław Walkowiak","doi":"10.3390/microorganisms13030619","DOIUrl":"10.3390/microorganisms13030619","url":null,"abstract":"<p><p>Oral cavity dysbiosis is associated with numerous inflammatory diseases, including diabetes, inflammatory bowel diseases, and periodontal disease. Changes in the oral microenvironment lead to bidirectional interactions between pathogens and individual host systems, which may induce systemic inflammation. There is increasing evidence linking the condition of the oral cavity with the most common causes of female infertility, such as polycystic ovary syndrome and endometriosis, as well as gestational complications, e.g., low birth weight, preterm delivery, and miscarriages. This review highlights the composition of the female oral microbiome in relation to infertility-related disorders, such as endometriosis and polycystic ovary syndrome, and provides a comprehensive overview of the current state of knowledge on the relationship between a dysbiotic oral microbiome, pregnancy, and its impact on the female reproductive tract.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of NatureKnit™, a Blend of Fruit and Vegetable Fibers Rich in Naturally Occurring Bound Polyphenols, on the Metabolic Activity and Community Composition of the Human Gut Microbiome Using the M-SHIME^® Gastrointestinal Model.","authors":"Marlies Govaert, Cindy Duysburgh, Brendan Kesler, Massimo Marzorati","doi":"10.3390/microorganisms13030613","DOIUrl":"10.3390/microorganisms13030613","url":null,"abstract":"<p><p>This study evaluated the impact of a proprietary blend of fruit and vegetable fibers rich in naturally occurring bound polyphenols (commercially marketed as NatureKnit^TM), compared to purified fibers (inulin and psyllium), on the human gut microbiome using the validated M-SHIME^® gastrointestinal model. A short-term single-stage colonic M-SHIME^® experiment (with fecal inoculum from three healthy human donors) was used to evaluate the test products compared to a negative control. Samples were assessed for pH, gas pressure, short-chain fatty acid (SCFA) production, lactate, and ammonium from 0 h to 48 h. Microbial community composition was assessed at 0 h (negative control only), 24 h, and 48 h (lumen) or 48 h (mucosal). All test products were fermented well in the colon as demonstrated by decreases in pH and increases in gas pressure over time; these changes occurred faster with the purified fibers, whereas NatureKnit™ demonstrated slow, steady changes, potentially indicating a gentler fermentation process. SCFA production significantly increased over the course of the 48 h experiment with all test products versus negative control. SCFA production was significantly greater with NatureKnit™ versus the purified fibers. Shifts in the microbial community composition were observed with all test products versus negative control. At the conclusion of the 48 h experiment, the absolute bacterial abundance and the richness of observed bacterial taxa in the lumen compartment was significantly greater with NatureKnit™ compared with inulin, psyllium, and negative control. Overall, NatureKnit™ demonstrated greater or similar prebiotic effects on study measures compared with established prebiotic fibers.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Affecting D-Lactic Acid Production by Flocculant <i>Saccharomyces cerevisiae</i> Under Non-Neutralizing Conditions.","authors":"Dianti Rahmasari, Prihardi Kahar, Arthur Vinícius de Oliveira, Filemon Jalu Nusantara Putra, Akihiko Kondo, Chiaki Ogino","doi":"10.3390/microorganisms13030618","DOIUrl":"10.3390/microorganisms13030618","url":null,"abstract":"<p><p>Integrating heterogeneous genes is widely used in metabolic engineering to produce D-lactic acid (D-LA), an essential compound in bioplastics and pharmaceuticals. However, research on the effects of integrating various loci on gene expression, especially regarding flocculation behavior, remains limited. This study constructed <i>Saccharomyces cerevisiae</i> strains by incorporating a codon-optimized <i>D-LDH</i> gene from <i>Leuconostoc pseudomesenteroides</i> (LpDLDH) into the specific genomic loci of the <i>CYB2</i>, <i>PDC1</i>, <i>MPC1</i>, <i>PDC6</i>, <i>ADH1</i>, and <i>PDC5</i> genes to redirect pyruvate toward lactic acid. Strains with the <i>LpDLDH</i> gene integrated at the <i>PDC1</i> locus achieved the highest D-LA titers (51 g/L) with minimal ethanol byproduct, followed by strains with integrations into the <i>CYB2</i> locus at 31.92 g/L, the <i>MPC1</i> locus at 10 g/L, and the <i>PDC6</i> locus at 0.026 g/L. In contrast, strains with <i>LpDLDH</i> integrated at the <i>ADH1</i> and <i>PDC5</i> loci failed to produce detectable levels of D-LA and exhibited a complete loss of flocculation. Gene expression analysis revealed a significant expression of genes related to flocculation (<i>FLO5</i>), stress adaptation (<i>HSP150</i>), and cell wall integrity (<i>YGP1</i>, <i>SED1</i>, and <i>SCW11</i>). The <i>CYB2</i>-integrating strain showed strong flocculant properties, contributing to its robustness. These findings highlight the influence of genomic locus selection on metabolic flux and stress adaptation, offering insights into optimizing D-LA production in flocculant <i>S. cerevisiae</i> yeast.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serogroup Distribution of <i>Leptospira</i> Among Humans and Rodents in Zakarpattia Oblast, Ukraine (2018-2023).","authors":"Pavlo Petakh, Oleksandr Kamyshnyi","doi":"10.3390/microorganisms13030614","DOIUrl":"10.3390/microorganisms13030614","url":null,"abstract":"<p><p>Leptospirosis is a zoonotic disease caused by <i>Leptospira</i> bacteria, which affects both humans and animals. This study investigated the prevalence of <i>Leptospira</i> serogroups in human and rodent reservoirs across Zakarpattia Oblast, Ukraine, from 2018 to 2023. The objective was to identify epidemiological patterns and assess potential public health risks. Data were sourced from the Public Health Center of Ukraine and regional surveillance initiatives, encompassing reported human cases and rodent-carrier detection. Six main serogroups-Icterohaemorrhagiae, Pomona, Grippotyphosa, Hebdomadis, Canicola, and Tarassovi-were included in the analysis. The results showed that Pomona and Hebdomadis serogroups became more common in 2023. The data from different districts also showed regional differences, with Icterohaemorrhagiae and Pomona being the most frequent serogroups in both humans and rodents. Other serogroups, like Grippotyphosa and Canicola, were found less often. Rodents may be an important source of leptospirosis in Zakarpattia. The growing number of cases in recent years shows the need for improved monitoring, control, and prevention in the region.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}