Microorganisms最新文献_第8页

Transcriptome of Kurthia gibsonii TYL-A1 Revealed the Biotransformation Mechanism of Tylosin.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-16 DOI: 10.3390/microorganisms12122597

Ye Wang, Cuizhu Zhao, Boyu Zhao, Xinran Duan, Peng Hao, Xiaojun Liang, Lianyu Yang, Yunhang Gao

{"title":"Transcriptome of Kurthia gibsonii TYL-A1 Revealed the Biotransformation Mechanism of Tylosin.","authors":"Ye Wang, Cuizhu Zhao, Boyu Zhao, Xinran Duan, Peng Hao, Xiaojun Liang, Lianyu Yang, Yunhang Gao","doi":"10.3390/microorganisms12122597","DOIUrl":"https://doi.org/10.3390/microorganisms12122597","url":null,"abstract":"Tylosin (TYL) pollution has aroused widespread concern, and its existence poses a serious threat to the environment and human health. Microbial degradation of antibiotics is considered to be an effective strategy to reduce the environmental impact of antibiotics, but its degradation mechanism is still unclear. In this study, transcriptome analysis was combined to explore the response mechanism of K. gibsonii strain TYL-A1 under TYL stress. The results showed that the strain showed a significant antioxidant response under TYL stress to cope with TYL-induced cell damage. TYL also increased the level of intracellular reactive oxygen species (ROS), damaged the integrity of the cell membrane, and inhibited the growth of strain TYL-A1. Transcriptome sequencing showed that under TYL exposure conditions, 1650 DEGs in strain TYL-A1 showed expression changes, of which 806 genes were significantly up-regulated and 844 genes were significantly down-regulated. Differentially expressed DEGs were significantly enriched in pathways related to metabolism, biosynthesis, and stress response, and tricarboxylic acid cycle, oxidative phosphorylation, and carbon metabolism genes were significantly up-regulated. In conclusion, this study provides novel insights regarding the degradation of TYL by K. gibsonii TYL-A1.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facultatively Anaerobic Staphylococci Enable Anaerobic Cutibacterium Species to Grow and Form Biofilms Under Aerobic Conditions.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-16 DOI: 10.3390/microorganisms12122601

Jeffrey B Kaplan, Michael Assa, Noor Mruwat, Miloslav Sailer, Suresh Regmi, Khalaf Kridin

{"title":"Facultatively Anaerobic Staphylococci Enable Anaerobic Cutibacterium Species to Grow and Form Biofilms Under Aerobic Conditions.","authors":"Jeffrey B Kaplan, Michael Assa, Noor Mruwat, Miloslav Sailer, Suresh Regmi, Khalaf Kridin","doi":"10.3390/microorganisms12122601","DOIUrl":"https://doi.org/10.3390/microorganisms12122601","url":null,"abstract":"Facultatively anaerobic Staphylococcus spp. and anaerobic Cutibacterium spp. are among the most prominent bacteria on human skin. Although skin microbes generally grow as multispecies biofilms, few studies have investigated the interaction between staphylococci and Cutibacterium spp. in dual-species biofilms. Here, we measured the mono- and dual-species biofilm formation of four staphylococcal species (S. epidermidis, S. hominis, S. capitis, and S. aureus) and two Cutibacterium spp. (C. acnes and C. avidum) cultured in vitro under both aerobic and anaerobic conditions. The biofilms were quantitated by rinsing them to remove planktonic cells, detaching the biofilm bacteria via sonication, and enumerating the cells by dilution plating. When cultured alone, staphylococci formed biofilms under both aerobic and anaerobic conditions, whereas Cutibacterium spp. formed biofilms only under anaerobic conditions. In co-culture, staphylococcal biofilm formation was unaffected by the presence of Cutibacterium spp., regardless of oxygen availability. However, Cutibacterium spp. biofilm formation was significantly enhanced in the presence of staphylococci, enabling robust growth under both anaerobic and aerobic conditions. Fluorescence confocal microscopy of the aerobic dual-species biofilms suggested that staphylococci create anaerobic niches at the base of the biofilm where C. acnes can grow. These findings demonstrate that staphylococci facilitate the colonization of Cutibacterium spp. in oxygen-rich environments, potentially explaining their presence in high numbers on the oxygen-exposed stratum corneum.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiproliferative and Morphological Analysis Triggered by Drugs Contained in the Medicines for Malaria Venture COVID-Box Against Toxoplasma gondii Tachyzoites.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-16 DOI: 10.3390/microorganisms12122602

Andréia Luiza Oliveira Costa, Mike Dos Santos, Giulia Caroline Dantas-Vieira, Rosálida Estevam Nazar Lopes, Rossiane Claudia Vommaro, Érica S Martins-Duarte

{"title":"Antiproliferative and Morphological Analysis Triggered by Drugs Contained in the Medicines for Malaria Venture COVID-Box Against Toxoplasma gondii Tachyzoites.","authors":"Andréia Luiza Oliveira Costa, Mike Dos Santos, Giulia Caroline Dantas-Vieira, Rosálida Estevam Nazar Lopes, Rossiane Claudia Vommaro, Érica S Martins-Duarte","doi":"10.3390/microorganisms12122602","DOIUrl":"https://doi.org/10.3390/microorganisms12122602","url":null,"abstract":"Toxoplasma gondii is a protozoan, and the etiologic agent of toxoplasmosis, a disease that causes high mortality in immunocompromised individuals and newborns. Despite the medical importance of toxoplasmosis, few drugs, which are associated with side effects and parasite resistance, are available for its treatment. Here, we show a screening of molecules present in COVID-Box to discover new hits with anti-T. gondii activity. COVID-Box contains 160 molecules with known or predicted activity against SARS-CoV-2. Our analysis selected 23 COVID-Box molecules that can inhibit the tachyzoite forms of the RH strain of T. gondii in vitro by more than 70% at 1 µM after seven days of treatment. The inhibitory curves showed that most of these molecules inhibited the proliferation of tachyzoites with IC50 values below 0.80 µM; Cycloheximide and (-)-anisomycin were the most active drugs, with IC50 values of 0.02 μM. Cell viability assays showed that the compounds are not toxic at active concentrations, and most are highly selective for parasites. Overall, all 23 compounds were selective, and for two of them (apilimod and midostaurin), this is the first report of activity against T. gondii. To better understand the effect of the drugs, we analyzed the effect of nine of them on the ultrastructure of T. gondii using transmission electron microscopy. After treatment with the selected drugs, the main changes observed in parasite morphology were the arrestment of cell division and organelle alterations.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Profiling of Endophytic Strain B.L.Ns.14 from Nigella sativa Reveals Potential for Agricultural Bioenhancement.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-16 DOI: 10.3390/microorganisms12122604

Dimitra Douka, Tasos-Nektarios Spantidos, Polina C Tsalgatidou, Panagiotis Katinakis, Anastasia Venieraki

{"title":"Whole-Genome Profiling of Endophytic Strain B.L.Ns.14 from Nigella sativa Reveals Potential for Agricultural Bioenhancement.","authors":"Dimitra Douka, Tasos-Nektarios Spantidos, Polina C Tsalgatidou, Panagiotis Katinakis, Anastasia Venieraki","doi":"10.3390/microorganisms12122604","DOIUrl":"https://doi.org/10.3390/microorganisms12122604","url":null,"abstract":"Endophytic microbes in medicinal plants often possess beneficial traits for plant health. This study focuses on the bacterial endophyte strain B.L.Ns.14, isolated from Nigella sativa leaves, which demonstrated multiple plant growth-promoting properties. In vitro tests showed that B.L.Ns.14 supports plant growth, colonization, and tolerance to abiotic stress. The strain also exhibited antifungal activity against phytopathogens such as Rhizoctonia solani, Colletotrichum acutatum, Verticillium dahliae, and Fusarium oxysporum f. sp. radicis-lycopersici. Whole-genome analysis, supported by ANI and dDDH values, identified B.L.Ns.14 as Bacillus halotolerans. Genome mining revealed 128 active carbohydrate enzymes (Cazymes) related to endophytism and biocontrol functions, along with genes involved in phosphate solubilization, siderophore and IAA production, biofilm formation, and motility. Furthermore, genes for osmolyte metabolism, Na+/H+ antiporters, and stress response proteins were also identified. The genome harbors 12 secondary metabolite biosynthetic gene clusters, including those for surfactin, plipastatin mojavensin, rhizocticin A, and bacilysin, known for their antagonistic effects against fungi. Additionally, B.L.Ns.14 promoted Arabidopsis thaliana growth under both normal and saline conditions, and enhanced Solanum lycopersicum growth via seed biopriming and root irrigation. These findings suggest that Bacillus halotolerans B.L.Ns.14 holds potential as a biocontrol and plant productivity agent, warranting further field testing.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Genome Sequence of a Novel Azospirillum Strain TA Isolated from Western Siberia Chernevaya Taiga Soil.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-16 DOI: 10.3390/microorganisms12122599

Mikhail Rayko, Irina Kravchenko, Alla Lapidus

{"title":"Complete Genome Sequence of a Novel Azospirillum Strain TA Isolated from Western Siberia Chernevaya Taiga Soil.","authors":"Mikhail Rayko, Irina Kravchenko, Alla Lapidus","doi":"10.3390/microorganisms12122599","DOIUrl":"https://doi.org/10.3390/microorganisms12122599","url":null,"abstract":"A whole genome sequence of a new strain of the nitrogen-fixing bacterium Azospirillum doebereinerae, known for its diverse plant growth-promoting bacteria (PGPB), was obtained for the first time. The strain, designated Azospirillum doebereinerae AT, was isolated during a soil analysis in the Chernevaya taiga of Western Siberia, a unique and fertile forest ecosystem known for its diverse plant growth-promoting bacteria (PGPB). The A. doebereinerae genome under study is fully assembled into seven circular molecules, none of which are unequivocally plasmids, with a total length of 6.94 Mb and a G + C content of 68.66%. A detailed phylogenomic analysis confirmed its placement within the genus Azospirillum, specifically closely related to A. doebereinerae GSF71T. Functional annotation revealed genes involved in nitrogen metabolism, highlighting the potential of strain TA as a biofertilizer and plant growth-promoting agent. The findings contribute to our understanding of the genomic diversity and metabolic potential of the Azospirillum genus, and they are of interest for further study in the field of comparative bacterial genomics, given the strain's multi-chromosomal genome structure.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11679736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas-Based Pen-Side Diagnostic Tests for Anaplasma marginale and Babesia bigemina.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-15 DOI: 10.3390/microorganisms12122595

Robert Muriuki, Maingi Ndichu, Samuel Githigia, Nicholas Svitek

{"title":"CRISPR-Cas-Based Pen-Side Diagnostic Tests for Anaplasma marginale and Babesia bigemina.","authors":"Robert Muriuki, Maingi Ndichu, Samuel Githigia, Nicholas Svitek","doi":"10.3390/microorganisms12122595","DOIUrl":"https://doi.org/10.3390/microorganisms12122595","url":null,"abstract":"Anaplasma marginale and Babesia bigemina are tick-borne pathogens, posing significant threats to the health and productivity of cattle in tropical and subtropical regions worldwide. Currently, detection of Babesia bigemina and Anaplasma marginale in infected animals relies primarily on microscopic examination of Giemsa-stained blood or organ smears, which has limited sensitivity. Molecular methods offer higher sensitivity but are costly and impractical in resource-limited settings. Following the development of a pen-side test for detecting Theileria parva infections in cattle, we have created two additional CRISPR-Cas12a assays targeting Anaplasma marginale and Babesia bigemina. The assays target the major surface protein 5 (MSP5) for A. marginale and rhoptry-associated protein 1a (RAP1a) for B. bigemina. These additional tests involve a 20 min recombinase polymerase amplification (RPA) reaction followed by a 60 min CRISPR-Cas12a detection with a lateral strip readout. Results demonstrate high specificity, with no cross-reactivity against other tick-borne parasites, and a limit of detection down to 102 DNA copies/µL of each target marker. The findings pave the way for sensitive and user-friendly pen-side tests to diagnose A. marginale and B. bigemina infections.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards Reliable Methodology: Microbiome Analysis of Fresh Frozen vs. Formalin-Fixed Paraffin-Embedded Bladder Tissue Samples: A Feasibility Study.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-15 DOI: 10.3390/microorganisms12122594

Dominik Enderlin, Uwe Bieri, Jana Gadient, Yasser Morsy, Michael Scharl, Jan Hendrik Rüschoff, Lukas John Hefermehl, Anna Nikitin, Janine Langenauer, Daniel Stephan Engeler, Beat Förster, Fabian Obrecht, Jonathan Surber, Thomas Paul Scherer, Daniel Eberli, Cédric Poyet

{"title":"Towards Reliable Methodology: Microbiome Analysis of Fresh Frozen vs. Formalin-Fixed Paraffin-Embedded Bladder Tissue Samples: A Feasibility Study.","authors":"Dominik Enderlin, Uwe Bieri, Jana Gadient, Yasser Morsy, Michael Scharl, Jan Hendrik Rüschoff, Lukas John Hefermehl, Anna Nikitin, Janine Langenauer, Daniel Stephan Engeler, Beat Förster, Fabian Obrecht, Jonathan Surber, Thomas Paul Scherer, Daniel Eberli, Cédric Poyet","doi":"10.3390/microorganisms12122594","DOIUrl":"https://doi.org/10.3390/microorganisms12122594","url":null,"abstract":"Studies have shown that the human microbiome influences the response to systemic immunotherapy. However, only scarce data exist on the impact of the urinary microbiome on the response rates of bladder cancer (BC) to local Bacillus Calmette-Guérin instillation therapy. We launched the prospective SILENT-EMPIRE study in 2022 to address this question. We report the results of the pilot study of SILENT-EMPIRE, which aimed to compare the microbiome between fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) samples in the cancerous tissue and adjacent healthy tissue of BC patients. Our results show that alpha diversity is increased in FF samples compared to FFPE (coverage index p = 0.041, core abundance index p = 0.008). No significant differences concerning alpha diversity could be detected between cancerous and non-cancerous tissue in the same BC patients. This study demonstrates that microbiome analysis from both FF and FFPE samples is feasible. Implementing this finding could aid in the translation of research findings into clinical practice.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Release of Biopolymers from Saccharomyces cerevisiae Biomass Through Thermal and Non-Thermal Technologies.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-15 DOI: 10.3390/microorganisms12122596

Marianna Ciccone, Muhammad Rehan Khan, Junior Bernardo Molina Hernandez, Joel Armando Njieukam, Lorenzo Siroli, Davide Gottardi, Rosalba Lanciotti, Pietro Rocculi, Francesca Patrignani

{"title":"Release of Biopolymers from Saccharomyces cerevisiae Biomass Through Thermal and Non-Thermal Technologies.","authors":"Marianna Ciccone, Muhammad Rehan Khan, Junior Bernardo Molina Hernandez, Joel Armando Njieukam, Lorenzo Siroli, Davide Gottardi, Rosalba Lanciotti, Pietro Rocculi, Francesca Patrignani","doi":"10.3390/microorganisms12122596","DOIUrl":"https://doi.org/10.3390/microorganisms12122596","url":null,"abstract":"Components of yeast cell walls, such as β-glucans and mannoproteins, show promise for developing sustainable biopolymers for food packaging. Efficient extraction, however, is challenging due to the complexity of the yeast cell wall. This study explored high-pressure homogenisation (HPH) and pulsed electric fields (PEFs), alone and with heat treatment (TT), on bakery yeast (BY) and brewery spent yeast (BSY) biomasses. In the treated samples we assessed carbohydrates, proteins, β-glucans, and mannoproteins and evaluated cell wall disruption microscopically. HPH caused complete cell disintegration, enhancing intracellular release, while PEF primarily permeabilised the membranes. Combined HPH and PEF treatments significantly increased cell wall stress, leading to partial disintegration. Notably, the β-glucans released reached 3.90 g/100 g dry matter in BY and 10.44 g/100 g dry matter in BSY, demonstrating significant extraction improvements. These findings highlight the potential of HPH and PEF for enhancing β-glucan recovery from yeast biomass, offering a promising route for sustainable biopolymer production for food packaging.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigenic Imprinting Dominates Humoral Responses to New Variants of SARS-CoV-2 in a Hamster Model of COVID-19.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-14 DOI: 10.3390/microorganisms12122591

Joran Degryse, Elke Maas, Ria Lassaunière, Katrien Geerts, Yana Kumpanenko, Birgit Weynand, Piet Maes, Johan Neyts, Hendrik Jan Thibaut, Yeranddy A Alpizar, Kai Dallmeier

{"title":"Antigenic Imprinting Dominates Humoral Responses to New Variants of SARS-CoV-2 in a Hamster Model of COVID-19.","authors":"Joran Degryse, Elke Maas, Ria Lassaunière, Katrien Geerts, Yana Kumpanenko, Birgit Weynand, Piet Maes, Johan Neyts, Hendrik Jan Thibaut, Yeranddy A Alpizar, Kai Dallmeier","doi":"10.3390/microorganisms12122591","DOIUrl":"https://doi.org/10.3390/microorganisms12122591","url":null,"abstract":"The emergence of SARS-CoV-2 variants escaping immunity challenges the efficacy of current vaccines. Here, we investigated humoral recall responses and vaccine-mediated protection in Syrian hamsters immunized with the third-generation Comirnaty® Omicron XBB.1.5-adapted COVID-19 mRNA vaccine, followed by infection with either antigenically closely (EG.5.1) or distantly related (JN.1) Omicron subvariants. Vaccination with the YF17D vector encoding a modified Gamma spike (YF-S0*) served as a control for SARS-CoV-2 immunity restricted to pre-Omicron variants. Our results show that both Comirnaty® XBB.1.5 and YF-S0* induce robust, however, poorly cross-reactive, neutralizing antibody (nAb) responses. In either case, total antibody and nAb levels increased following infection. Intriguingly, the specificity of these boosted nAbs did not match the respective challenge virus, but was skewed towards the primary antigen used for immunization, suggesting a marked impact of antigenic imprinting, confirmed by antigenic cartography. Furthermore, limited cross-reactivity and rapid decline in nAbs induced by Comirnaty® XBB.1.5 with EG.5.1 and, more concerning, JN.1, raises doubts about sustained vaccine efficacy against recent circulating Omicron subvariants. In conclusion, we demonstrate that antigenic imprinting plays a dominant role in shaping humoral immunity against emerging SARS-CoV-2 variants. Future vaccine design may have to address two major issues: (i) overcoming original antigenic sin that limits the breadth of a protective response towards emerging variants, and (ii) achieving sustained immunity that lasts for at least one season.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Distiller's Grains Biochar and Lactobacillus plantarum on the Remediation of Cd-Pb-Zn-Contaminated Soil and Growth of Sorghum-Sudangrass.

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-12-14 DOI: 10.3390/microorganisms12122592

Guangxu Zhu, Yufeng Li, Dandan Cheng, Rongkun Chen, Yunyan Wang, Qiang Tu

{"title":"Effects of Distiller's Grains Biochar and Lactobacillus plantarum on the Remediation of Cd-Pb-Zn-Contaminated Soil and Growth of Sorghum-Sudangrass.","authors":"Guangxu Zhu, Yufeng Li, Dandan Cheng, Rongkun Chen, Yunyan Wang, Qiang Tu","doi":"10.3390/microorganisms12122592","DOIUrl":"https://doi.org/10.3390/microorganisms12122592","url":null,"abstract":"Soil contamination with heavy metals is a significant environmental issue that adversely affects plant growth and agricultural productivity. Biochar and microbial inoculants have emerged as a promising approach to solving this problem, and previous studies have focused more on the remediation effects of single types of materials on heavy metal soil pollution. This study examined the impact of both standalone and combined applications of distiller's grains biochar, Lactobacillus plantarum thallus, and the bacterial supernatant on the availability of cadmium (Cd), lead (Pb), and zinc (Zn) in soil, its physicochemical features, and its enzyme activities; this study also examined the growth, physiological and biochemical characteristics, and heavy metal accumulation of Sorghum-sudangrass. The findings suggest that the application of distiller's grains biochar, Lactobacillus plantarum thallus, and the bacterial supernatant can improve the soil's physical and chemical properties and enhance soil enzyme activity while reducing the availability of heavy metals in the soil. Furthermore, the addition of these materials promoted plant growth, increased stress resistance, and significantly decreased the accumulation of heavy metals in the plants. A thorough analysis of the results shows that applying 0.025% Lactobacillus plantarum thallus along with 4.4% distiller's grains biochar produced the best results.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0