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A Novel Neotropical Bacillus siamensis Strain Inhibits Soil-Borne Plant Pathogens and Promotes Soybean Growth. 一株新热带芽孢杆菌抑制土传植物病原体并促进大豆生长。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061366
Rodrigo F Moreira, Elizabeth B E Pires, Odaiza F Sousa, Giselly B Alves, Luis O Viteri Jumbo, Gil R Santos, Luís J Maia, Bergmann M Ribeiro, Guy Smagghe, Elvio H B Perino, Rudolf Hausmann, Eugenio E Oliveira, Raimundo W S Aguiar
{"title":"A Novel Neotropical <i>Bacillus siamensis</i> Strain Inhibits Soil-Borne Plant Pathogens and Promotes Soybean Growth.","authors":"Rodrigo F Moreira, Elizabeth B E Pires, Odaiza F Sousa, Giselly B Alves, Luis O Viteri Jumbo, Gil R Santos, Luís J Maia, Bergmann M Ribeiro, Guy Smagghe, Elvio H B Perino, Rudolf Hausmann, Eugenio E Oliveira, Raimundo W S Aguiar","doi":"10.3390/microorganisms13061366","DOIUrl":"10.3390/microorganisms13061366","url":null,"abstract":"<p><p>Soil-borne fungal pathogens such as <i>Sclerotium</i> spp., <i>Rhizoctonia</i> spp., and <i>Macrophomina</i> spp. pose significant threats to global agriculture, with soybean crops among the most severely affected due to damping-off disease. These pathogens cause substantial yield losses, making their management a critical concern. In this study, we investigated the potential of <i>Bacillus siamensis</i> BCL, a novel Neotropical strain, as an eco-friendly solution for managing <i>Sclerotium</i>, <i>Rhizoctonia</i>, and <i>Macrophomina</i> species. The strain exhibited strong antifungal activity, significantly inhibiting fungal growth in vitro, with the greatest suppression observed against <i>Macrophomina</i> spp., reaching up to 81%. In vivo assays further confirmed the biocontrol potential of <i>B. siamensis</i>. When applied at 10<sup>6</sup> colony-forming units (CFU)/mL, the strain reduced disease symptoms and improved plant growth parameters-including root length, shoot biomass, and leaf number-compared to untreated, infected controls. The protective effect varied by pathogen, with the most significant recovery in root length observed against <i>Macrophomina</i> spp. (85%) and <i>Sclerotium</i> spp. (78%). In preventive treatments, fermentation extracts of the <i>B. siamensis</i> strain suppressed disease progression, although they did not promote seedling growth. A genomic analysis of <i>B. siamensis</i> BCL revealed genes encoding antimicrobial secondary metabolites, including terpenes, fengycins, and surfactins. These findings highlight <i>B. siamensis</i> BCL as a promising candidate for sustainable crop protection and a valuable resource for developing novel antimicrobial strategies in agriculture.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence of Malodorous Chloroanisoles in "Mold Houses" Was Omitted When Indoor Air Research Evolved. 在室内空气研究的发展过程中,“霉菌屋”中含有氯苯甲醚的证据被忽略了。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-12 DOI: 10.3390/microorganisms13061363
Johnny C Lorentzen, Gunnar Johanson
{"title":"Evidence of Malodorous Chloroanisoles in \"Mold Houses\" Was Omitted When Indoor Air Research Evolved.","authors":"Johnny C Lorentzen, Gunnar Johanson","doi":"10.3390/microorganisms13061363","DOIUrl":"10.3390/microorganisms13061363","url":null,"abstract":"<p><p>Herein, we address the peculiar lack of scientific reporting on odor potent chloroanisoles (CAs) in the built environment. We have searched and critically examined sources beyond peer-reviewed scientific journals, namely research conferences, parliamentary records, newspaper articles, and cartoons. We provide evidence that CAs evolved on a large scale in Swedish buildings in the early 1970s and evoked a typical sticky malodor that was attributed to mold and gave rise to the term \"mold houses\". The term first appeared in Swedish newspapers in 1978, and the media attention increased rapidly. The malodorous \"mold houses\" reached the Swedish parliament and led to economic compensation for afflicted homeowners. The \"mold houses\" became \"sick houses\" as researchers, predominantly from Sweden, introduced and became world leaders on the \"sick buildings syndrome\" (SBS). Researchers became aware of the CAs but did not mention them in peer-reviewed articles, just as they did not mention a well-known source of the sticky malodor, namely, legacy preserved wood where CAs were formed through microbial methylation of toxic chlorophenols (CPs). Thus, the mold story from the early 1970s was maintained and prevented the malodorous CAs from becoming recognized as indicators of the presence of hazardous CPs. Our study is the first to report the impact of an indoor malodor, not only on a few people, but on society.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Early Response of Rhizosphere Microbial Community Network Characteristics to Thinning Intensity in Pinus massoniana Plantations. 马尾松人工林根际微生物群落网络特征对间伐强度的早期响应
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061357
Size Liu, Haifeng Yin, Yu Su, Xianwei Li, Chuan Fan
{"title":"Early Response of Rhizosphere Microbial Community Network Characteristics to Thinning Intensity in <i>Pinus massoniana</i> Plantations.","authors":"Size Liu, Haifeng Yin, Yu Su, Xianwei Li, Chuan Fan","doi":"10.3390/microorganisms13061357","DOIUrl":"10.3390/microorganisms13061357","url":null,"abstract":"<p><p>Rhizosphere microorganisms mediate the material exchange and chemical cycling between plant roots and soil. However, the response mechanisms of the rhizosphere microbial community, especially its co-occurrence patterns, to thinning remain poorly understood. We investigated the rhizosphere microbial communities of <i>Pinus massoniana</i> under different thinning intensities, including control (CK, 0%), light-intensity thinning (LIT, 10%), moderate-intensity thinning (MIT, 30%), and high-intensity thinning (HIT, 50%). Basic taxonomic information was obtained through high-throughput sequencing, while R software was utilized to identify thinning-sensitive operational taxonomic units (tsOTUs), construct co-occurrence networks, and perform other statistical analyses. Although no discernible patterns were observed in α-diversity changes, the Kruskal-Wallis test indicated that season was the primary factor driving α-diversity variation. Meanwhile, thinning intensity significantly shaped the rhizosphere microbial community structures, with each intensity harboring a specific tsOTUs subset. Although the top three modules of the meta-co-occurrence networks in summer and winter exhibited consistent tsOTU composition, winter triggered changes in network connectivity. Regardless of summer or winter, the number of network nodes under MIT was the highest. Additionally, after thinning, the relative abundances of most keystone taxa declined; however, MIT facilitated the enrichment of certain keystone taxa. Collectively, thinning profoundly shapes microbial community composition and network characteristics. Moderate thinning intensity may represent the optimal thinning intensity for the studied <i>P. massoniana</i> plantations.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Impact of Tank Disinfectants on the Development of Microbiota in Gilthead Seabream (Sparus aurata) Larviculture Systems. 池内消毒剂对黄颡鱼(Sparus aurata)幼虫养殖系统微生物群发育的影响。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061359
Georgia Apostolopoulou, Naima Bel Mokhtar, Elias Asimakis, Eva Dionyssopoulou, Kosmas Toskas, George Koumoundouros, George Tsiamis, Panagiota Stathopoulou
{"title":"The Impact of Tank Disinfectants on the Development of Microbiota in Gilthead Seabream (<i>Sparus aurata</i>) Larviculture Systems.","authors":"Georgia Apostolopoulou, Naima Bel Mokhtar, Elias Asimakis, Eva Dionyssopoulou, Kosmas Toskas, George Koumoundouros, George Tsiamis, Panagiota Stathopoulou","doi":"10.3390/microorganisms13061359","DOIUrl":"10.3390/microorganisms13061359","url":null,"abstract":"<p><p>Aquaculture play a vital role in enhancing human nutrition by producing commercially valuable fish, with gilthead seabream (<i>Sparus aurata</i>) being a key species in the Mediterranean region. In seabream larviculture, disinfection is commonly used to control pathogens and prevent microbial imbalances. However, this process may also remove beneficial microbiota that contribute to ecosystem stability. This study aims to investigate the impact of tank disinfection operations on the bacterial communities associated with seabream larvae and their rearing water in a commercial hatchery using 16S rRNA amplicon sequencing. For further comparison, the bacterial communities present in eggs and feed were also analyzed for comparison. Results showed that the use of different disinfectants significantly altered the bacterial composition of the larvae, while the duration of the dry period had no measurable effect. Across all larval samples, the phylum Pseudomonadota dominated, with members of the genus <i>Psychrobacter</i> consistently detected regardless of disinfection treatment. This suggests that <i>Psychrobacter</i> may be transmitted from eggs or acquired through the feed, mainly rotifers and <i>Artemia nauplii</i>. In contrast, the bacterial communities in the rearing water were more diverse and showed only minor differences in relative abundance across disinfection methods.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences in Ct Values in qPCR Tests for the Diagnosis of Mpox: Results of a Cross-Sectional Study. 诊断m痘的qPCR检测中Ct值的差异:一项横断面研究的结果。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061355
Clara Esperanza Santacruz Tinoco, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Julio Elias Alvarado Yaah, Bernardo Martínez Miguel, Yu-Mei Anguiano Hernández, Alfonso Vallejos Parás, José Esteban Muñoz Medina, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Yadira Pérez Andrade
{"title":"Differences in Ct Values in qPCR Tests for the Diagnosis of Mpox: Results of a Cross-Sectional Study.","authors":"Clara Esperanza Santacruz Tinoco, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Julio Elias Alvarado Yaah, Bernardo Martínez Miguel, Yu-Mei Anguiano Hernández, Alfonso Vallejos Parás, José Esteban Muñoz Medina, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Yadira Pérez Andrade","doi":"10.3390/microorganisms13061355","DOIUrl":"10.3390/microorganisms13061355","url":null,"abstract":"<p><p>There is little evidence regarding whether Ct values are influenced by age, type of care, and the timing from the date of symptom onset to obtaining samples. The aim of this study was to determine the differences in the qPCR Ct values for mpox by age, type of care, and sampling opportunity. This descriptive, retrospective study analyzed the qPCR Ct values for mpox. The odds of a low Ct value were assessed by age group, type of care, sampling opportunity, and type of exudate. To determine the odds of a low Ct value, a multivariate bivariate analysis of the variables was performed, and a logistic regression model was developed. A total of 520 positive mpox tests were identified; the general median Ct value was 23.67. There was a difference in Ct values between inpatients and outpatients. Pharyngeal exudate samples had the highest Ct value (34.24), and pustule and scab exudate samples had the lowest values. Samples with a low Ct value (OR = 2.45, 95% CI: 1.13-5.29) were associated with hospital care. Low Ct values were more likely for samples from individuals being cared for in a hospital setting and with pustule skin lesions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Progress on the Application of Neutralizing Nanobodies in the Prevention and Treatment of Viral Infections. 中和性纳米体在防治病毒感染中的应用研究进展。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061352
Qingling Duan, Tong Ai, Yingying Ma, Ruoyu Li, Hanlin Jin, Xingyi Chen, Rui Zhang, Kunlu Bao, Qi Chen
{"title":"Research Progress on the Application of Neutralizing Nanobodies in the Prevention and Treatment of Viral Infections.","authors":"Qingling Duan, Tong Ai, Yingying Ma, Ruoyu Li, Hanlin Jin, Xingyi Chen, Rui Zhang, Kunlu Bao, Qi Chen","doi":"10.3390/microorganisms13061352","DOIUrl":"10.3390/microorganisms13061352","url":null,"abstract":"<p><p>Public health crises triggered by viral infections pose severe threats to individual health and disrupt global socioeconomic systems. Against the backdrop of global pandemics caused by highly infectious diseases such as COVID-19 and Ebola virus disease (EVD), the development of innovative prevention and treatment strategies has become a strategic priority in the field of biomedicine. Neutralizing antibodies, as biological agents, are increasingly recognized for their potential in infectious disease control. Among these, nanobodies (Nbs) derived from camelid heavy-chain antibodies exhibit remarkable technical advantages due to their unique structural features. Compared to traditional neutralizing antibodies, nanobodies offer significant cost-effectiveness in production and enable versatile administration routes (e.g., subcutaneous injection, oral delivery, or aerosol inhalation), making them particularly suitable for respiratory infection control and resource-limited settings. Furthermore, engineered modification strategies-including multivalent constructs, multi-epitope recognition designs, and fragment crystallizable (Fc) domain fusion-effectively enhance their neutralizing activity and suppress viral immune escape mechanisms. Breakthroughs have been achieved in combating pathogens such as the Ebola virus and SARS-CoV-2, with mechanisms involving the blockade of virus-host interactions, induction of viral particle disintegration, and enhancement of immune responses. This review comprehensively discusses the structural characteristics, high-throughput screening technologies, and engineering strategies of nanobodies, providing theoretical foundations for the development of novel antiviral therapeutics. These advances hold strategic significance for addressing emerging and re-emerging infectious diseases.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction Between Enterococcus faecalis and Fusobacterium nucleatum Regulated Macrophage Transcriptional Profiling and Reprogrammed Cellular Immune and Metabolic Response. 粪肠球菌和核梭杆菌相互作用调节巨噬细胞转录谱和重编程细胞免疫和代谢反应。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061351
Jingheng Liang, Wenling Huang, Poukei Chan, Lihong Guo
{"title":"Interaction Between <i>Enterococcus faecalis</i> and <i>Fusobacterium nucleatum</i> Regulated Macrophage Transcriptional Profiling and Reprogrammed Cellular Immune and Metabolic Response.","authors":"Jingheng Liang, Wenling Huang, Poukei Chan, Lihong Guo","doi":"10.3390/microorganisms13061351","DOIUrl":"10.3390/microorganisms13061351","url":null,"abstract":"<p><p>Refractory apical periodontitis (RAP), a persistent infection after root canal treatment, still has no effective treatment. <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) and <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>) are frequently detected in the lesion. We previously found that coaggregation altered gene expression of <i>E. faecalis</i> and <i>F. nucleatum</i> and promoted immune evasion by suppressing pro-inflammatory cytokine secretion of macrophages (Mφs) while sustaining low-grade inflammation. In this study, we further investigated the synergistic effect of coaggregated <i>E. faecalis</i> and <i>F. nucleatum</i> on modulating Mφ immune and metabolic responses. Using transmission electron microscope, flow cytometry, RNA-seq and functional assays, we demonstrated that coaggregated <i>E. faecalis</i> and <i>F. nucleatum</i> caused nuclear shrinkage and increased mitochondria in Mφ while inducing M1 polarization, ROS production, and lipid accumulation of Mφ. The key driver genes causing the difference between single species-infected and coaggregated bacteria-infected Mφ mainly included IFN-stimulated genes and genes related to the chemokine signaling pathway. These findings indicate that the synergism of <i>E. faecalis</i> and <i>F. nucleatum</i> can regulate the immune and metabolic response of Mφ, offering novel insights into therapeutic targets for refractory apical periodontitis.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Animal Models of Pathogenic New World Arenaviruses. 致病性新世界沙粒病毒的动物模型。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061358
Alexander V Alvarado, Robert W Cross, Thomas W Geisbert, Courtney Woolsey
{"title":"Animal Models of Pathogenic New World Arenaviruses.","authors":"Alexander V Alvarado, Robert W Cross, Thomas W Geisbert, Courtney Woolsey","doi":"10.3390/microorganisms13061358","DOIUrl":"10.3390/microorganisms13061358","url":null,"abstract":"<p><p>Since the emergence of Junín virus in 1953, pathogenic New World arenaviruses have remained a public health concern. These viruses, which also include Machupo virus, Guanarito virus, Sabiá virus, and Chapare virus, cause acute viral hemorrhagic fever and neurological complications, resulting in significant morbidity and mortality. Given the dearth of licensed therapeutics or vaccines against these pathogens, animal models of infection that recapitulate human manifestations of disease remain critically important to the development of efficacious medical countermeasures. Rodents and non-human primates have been successfully used to model human New World arenaviral infections, with guinea pigs, rhesus macaques, and cynomolgus macaques being the most successful models of infection for most major pathogenic New World arenaviruses. Here, we provide a highly comprehensive review of publicly reported animal models of pathogenic New World arenavirus infections, with a discussion of advantages and disadvantages for each model.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Enzyme-Mediated Duplex Exponential Amplification Assay for Detection and Identification of Meloidogyne enterolobii in Field. 酶介导双指数扩增法在田间检测和鉴定肠曲线虫的建立。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061353
Bingxue Sun, Bo Gao, Rongyan Wang, Shulong Chen, Xiuhua Li, Yonghao Dong, Juan Ma
{"title":"Development of Enzyme-Mediated Duplex Exponential Amplification Assay for Detection and Identification of <i>Meloidogyne enterolobii</i> in Field.","authors":"Bingxue Sun, Bo Gao, Rongyan Wang, Shulong Chen, Xiuhua Li, Yonghao Dong, Juan Ma","doi":"10.3390/microorganisms13061353","DOIUrl":"10.3390/microorganisms13061353","url":null,"abstract":"<p><p>The root-knot nematode <i>Meloidogyne enterolobii</i> has emerged as a devastating pathogen in global agricultural systems. Its geographic distribution is progressively expanding from tropical to temperate zones, leading to difficulties in discerning the symptoms it causes from those of congeners such as <i>M. incognita</i>. Currently, some molecular diagnostic technologies (e.g., qPCR) have been established for detecting <i>M. enterolobii</i>, but these methods fail to meet field-based detection demands due to their reliance on laboratory-grade thermocyclers. We thus developed a method for detecting <i>M. enterolobii</i> based on enzyme-mediated duplex exponential amplification (EmDEA) technologies to address this issue. The EmDEA detection method demonstrated strict specificity for the target species, showing no amplification in 13 non-target nematodes or host tissue samples. Sensitivity analyses revealed detection limits of 3.6 × 10<sup>-4</sup> ng/μL (purified DNA), 1/1000 of an individual nematode (single-organism detection), 8.97 nematodes/g sweet potato, and 4.08 nematodes/100 g soil, achieving equivalent performance to qPCR. Field validation confirmed successful on-site detection, with significantly higher nematode loads in root tissues (50.41-97.62 nematodes/g) than in rhizospheric soil (1.07-1.28 nematodes/g). The established detection method employs a 42 °C isothermal amplification technology paired with a palm-sized thermal module, enabling field-deployable detection. Its unique duplex exponential amplification mechanism achieves threshold determination 10 cycles (~10 min) faster than conventional qPCR. When integrated with rapid DNA extraction protocols, the entire workflow is completed within 40 min, improving detection efficiency. This study provides a molecular tool for the precise monitoring of <i>M. enterolobii</i>, offering critical support for formulating targeted control strategies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nasal Colonizers from Sows in the Federal District of Brazil Showed a Diverse Phenotypic Resistance Profile. 来自巴西联邦区母猪的鼻腔定植菌显示出不同的表型抗性谱。
IF 4.1 2区 生物学
Microorganisms Pub Date : 2025-06-11 DOI: 10.3390/microorganisms13061354
Luciana Lana Rigueira, Fabiano José Ferreira de Sant'Ana, Bruno Stéfano Lima Dallago, Rômulo Salignac Araújo de Faria, Maurício Macedo Rodrigues, Pau Obregon-Gutierrez, Virginia Aragon, Simone Perecmanis
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