Microorganisms最新文献

{"title":"Molecular Characterization of <i>Streptococcus pyogenes</i> Isolates Recovered from Hospitalized Patients During the Years 2023-2024.","authors":"Adile A Muhtarova, Vasil S Boyanov, Alexandra S Alexandrova, Raina T Gergova","doi":"10.3390/microorganisms13092148","DOIUrl":"10.3390/microorganisms13092148","url":null,"abstract":"<p><p>In recent years, the incidence of severe <i>Streptococcus pyogenes</i> (group A Streptococcus, GAS) infections has been increasing worldwide, similar to trends observed prior to the COVID-19 pandemic, alongside a rise in antibiotic resistance. In the present study, we identified the circulating 12 <i>emm</i> types and 8 clusters of 70 GAS isolates among inpatients, investigated their association with antibiotic susceptibility, and compared these findings with earlier research conducted in our country. The predominant <i>emm</i> types and clusters were <i>emm</i>1, <i>emm</i>3, and <i>emm</i>4, and A-C3, E4, and, A-C5, respectively. <i>emm</i>1 was the most common among patients with skin and soft tissue infections or pneumonia, while <i>emm</i>3 was detected in patients with peritonsillar abscesses. All isolates demonstrated susceptibility to penicillin and linezolid, whereas the prevalence of resistance to macrolides, lincosamides, and tetracyclines was found to be 14.3%, 14.3%, and 18.6%, respectively. A notable change in the distribution of <i>emm</i>-types/clusters has been observed, with emm1/A-C3 now identified as the most prevalent, differing from our previous study conducted in the pre-COVID-19 period. Additionally, we noted a decrease in resistance to macrolides attributed to a lower prevalence of <i>emm</i>28 clone. The current research is important for monitoring isolates responsible for severe infections, which is crucial for GAS surveillance.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Ancient Philosophy to Endosymbiotic Theory: The Bacterial Origin and Key Role of Mitochondria in Immune Responses.","authors":"Alexandra Mpakosi, Christiana Kaliouli-Antonopoulou, Vasileios Cholevas, Stamatios Cholevas, Ioannis Tzouvelekis, Maria Mironidou-Tzouveleki, Emmanuel A Tsantes, Deny Tsakri, Marianna Vlachaki, Stella Baliou, Petros Ioannou, Rozeta Sokou, Stefanos Bonovas, Andreas G Tsantes","doi":"10.3390/microorganisms13092149","DOIUrl":"10.3390/microorganisms13092149","url":null,"abstract":"<p><p>The endosymbiotic theory, which is the crucial starting point of eukaryogenesis, was first mentioned in the philosophy of the pre-Socratic Greek philosopher Empedocles. According to him, everything merges into units with differential survival. Similarly, during eukaryogenesis, the fusion of two distinct units resulted in the creation of a new cell type that possessed a newly formed organelle, the mitochondrion. Since then, the mitochondrion has been a key regulator of health and immunity. Furthermore, many of its characteristics and functions are due to its endosymbiotic bacterial origin. For example, it possesses damage-associated molecular patterns that can activate inflammatory signaling pathways, has circular DNA with CpG-rich motifs, as well as a double phospholipid membrane, and divides by fission. Mitochondrial function plays a critical role in maintaining cellular homeostasis, as they meet the cell's energy needs and regulate many of its functions. However, after cellular damage due to infection, radiation, or toxins, mitochondrial stress and dysfunction can occur and mitochondrial DNA can be released into the cytosol. Cytosolic mitochondrial DNA can then activate proinflammatory signaling pathways, mediated by TLR9 and cGAS, as well as inflammasomes, triggering inflammation and autoimmunity.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals from <i>Euclea natalensis</i> Modulate Th17 Differentiation, HIV Latency, and Comorbid Pathways: A Systems Pharmacology and Thermodynamic Profiling Approach.","authors":"Ernest Oduro-Kwateng, Nader E Abo-Dya, Mahmoud E Soliman, Nompumelelo P Mkhwanazi","doi":"10.3390/microorganisms13092150","DOIUrl":"10.3390/microorganisms13092150","url":null,"abstract":"<p><p>HIV/AIDS remains a major global health challenge, with immune dysfunction, chronic inflammation, and comorbidities sustained by latent viral reservoirs that evade antiretroviral therapy. <i>Euclea natalensis</i>, a medicinal plant widely used in Southern African ethnomedicine, remains underexplored for its potential against HIV. An integrative systems pharmacology and molecular modeling framework was employed, including ADME profiling, target mapping, PPI network analysis, GO and KEGG pathway enrichment, BA-TAR-PATH analysis, molecular docking, MD simulations, and MM/GBSA calculations, to investigate the mechanistic roles of <i>E. natalensis</i> phytochemicals in HIV pathogenesis. Sixteen phytochemicals passed ADME screening and mapped to 313 intersecting host targets, yielding top ten hub genes with GO annotations in immune-metabolic, apoptotic, and nuclear signaling pathways. KEGG analysis revealed the enrichment of HIV-relevant pathways, including Th17 cell differentiation (hsa04659), PD-L1/PD-1 checkpoint (hsa05235), IL-17 signaling (hsa04657), HIF-1 signaling pathway (hsa04066), and PI3K-Akt (hsa04151). Lead phytochemicals, diospyrin and galpinone, strongly targeted key hub proteins (<i>NFκβ1</i>, <i>STAT3</i>, <i>MTOR</i>, <i>HSP90AA1</i>, and <i>HSP90AB1</i>), demonstrating favorable binding affinities, conformational stability, and binding free energetics compared to reference inhibitors. <i>E. natalensis</i> phytochemicals may modulate Th17 differentiation, HIV latency circuits, and comorbidity-linked signaling by targeting multiple host pathways, supporting their potential as multi-target therapeutic candidates for adjunct HIV/AIDS treatment and immunotherapy.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Gut Microbiota and Its Metabolites in Mitigating Radiation Damage.","authors":"Hansheng Zhu, Xin Yan, Hao Shi, Yiping Chen, Changyi Huang, Yue Zhou, Shiying Yan, Nan Zhang, Jia Wang, Jian Zhang, Chaoyi Han, Qian Chen, Jian Zhao, Mei Cao","doi":"10.3390/microorganisms13092151","DOIUrl":"10.3390/microorganisms13092151","url":null,"abstract":"<p><p>With the widespread use of ionizing radiation (IR) in medical and industrial settings, irradiation has become increasingly common, posing significant risks to human health. Among the various organs affected, the gut is particularly sensitive to radiation-induced damage, leading to conditions such as radiation-induced intestinal damage (RIID). Recent studies have emphasized the critical role of gut microbiota and its metabolites in mitigating radiation-induced injury. This review discusses the effects of IR on the mammalian and human gut microbiota. We examine the dynamics of gut microbiota composition during and after irradiation, and emphasize the protective role of the gut flora and the metabolites in the pathophysiological mechanisms exhibited during radiation injury. In addition, this article investigates how specific metabolites, such as short-chain fatty acids and indole derivatives, may contribute to the mitigation of inflammation and promotion of gut barrier integrity. In addition, various therapeutic strategies based on modulating the gut microbiota, such as probiotics, antibiotics, and fecal microbiota transplantation, are discussed to understand their potential to prevent or mitigate RIID. Understanding the interactions between IR, gut microbiota and their metabolites provides new avenues for developing innovative therapeutic approaches to improve patient outcomes during and after radiotherapy. Future research directions could focus on optimizing microbiota-based therapies and exploring the role of diet and lifestyle in enhancing intestinal health during irradiation.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Microbiome as an Immunoregulatory Axis: Mechanisms, Dysbiosis, and Therapeutic Modulation.","authors":"Matías Cortés, Paula Olate, Rodrigo Rodriguez, Rommy Diaz, Ailín Martínez, Genisley Hernández, Nestor Sepulveda, Erwin A Paz, John Quiñones","doi":"10.3390/microorganisms13092147","DOIUrl":"10.3390/microorganisms13092147","url":null,"abstract":"<p><p>The human microbiome plays a central role in modulating the immune system and maintaining immunophysiological homeostasis, contributing to the prevention of immune-mediated diseases. In particular, the gut microbiota is a key ecosystem for immune system maturation, especially in early life. This review aimed to analyze the molecular and cellular mechanisms linking the microbiome to immune and neuronal functions, as well as the impact of dysbiosis and emerging therapeutic strategies targeting the microbiome. The analysis was based on scientific databases, prioritizing studies published since 2000, with special emphasis on the past decade. The microbiome influences immune signaling through microorganism-associated molecular patterns (MAMPs) and pattern recognition receptors (PRRs), including Toll-like receptors (TLRs). Additionally, microbial metabolites-such as short-chain fatty acids (SCFAs), tryptophan derivatives, and secondary bile acids-exert significant immunomodulatory effects. The intestinal epithelial barrier is also described as an active immunological interface contributing to systemic regulation. The literature highlights innovative therapies, including fecal microbiota transplantation (FMT), probiotics, and microbiome editing with CRISPR-Cas technologies. These strategies aim to restore microbial balance and improve immune outcomes. The growing body of evidence positions the microbiome as a valuable clinical and diagnostic target, with significant potential for application in personalized medicine.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145175971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Region-Specific Gut Microbiome Variation Between Changle Geese and Yellow-Feathered Broilers: Correlations with Growth and Intestinal Development.","authors":"Dingcheng Ye, Jianxing Qiu, Zitao Fan, Luwei Zhu, Chengyong Lv, Pingting Guo","doi":"10.3390/microorganisms13092145","DOIUrl":"10.3390/microorganisms13092145","url":null,"abstract":"<p><p>This study comparatively analyzed the spatial heterogeneity of the gut microbiome across gastrointestinal segments in Changle geese versus yellow-feathered broilers to discover their links with growth and intestinal development. Twelve 63-day-old male yellow-feathered broilers and twelve 70-day-old male Changle geese were selected. Body weight (BW), slaughter weight (SW), absolute lengths of the small intestine (LSI) and cecum (LC), and their relative lengths normalized to body size (RLSI/RLC) were measured. Additionally, 16S rDNA sequencing of crop, proventriculus, gizzard, jejunum, cecum, and rectum microbiota was conducted to assess microbial diversity, composition, and its correlation with phenotypes. Results demonstrated higher BW, SW, LSI, LC and lower RLSI and RLC in geese versus broilers (<i>p</i> < 0.001). Alpha diversity analysis revealed lower microbial richness and diversity in broilers across most gastrointestinal segments (<i>p</i> < 0.05), while beta diversity analysis confirmed distinct community structures between two species (<i>p</i> = 0.001). Firmicutes dominated broiler gut microbiota (94.49%), whereas geese exhibited greater phylum-level diversity (<i>p</i> < 0.05). Random forestry analysis identified Top 15 core Amplicon Sequencing Variants in both the cecum and rectum, with ASV12260 (unclassified Lachnospiraceae) and ASV12412 (<i>uncultured Faecalibacterium</i> sp.) as key biomarkers. Correlation analyses found 21 phenotype-related ASVs (<i>p</i> < 0.05). Specially, two <i>Lactobacillus ingluviei</i> strains showed negatively correlated with LSI and RLSI in the chicken foregut (<i>p</i> < 0.05). And two <i>Gallibacterium anatis</i> strains were associated with RLSI, with one strain also showing an inverse correlation with LSI in the goose foregut (<i>p</i> < 0.05). Interestingly, one <i>Peptococcus</i> strain was negatively correlated with BW and SW, while the other was inversely associated with LC and RLC in the goose hindgut (<i>p</i> < 0.05). These findings provide insights into species-specific distribution patterns of gut microbiota across poultry species and their correlation with growth performance and intestinal development, developing a theoretical foundation for advancing avian digestive physiology research and optimizing feeding strategies.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal Parasites, Ectoparasites, and Fungi in Rabbits Attending Clinical Consultations and from Private Owners and Breeders in Portugal.","authors":"Carolina Vale, João Lozano, Ana Reisinho, Mariana Louro, Manuela Oliveira, Eva Cunha, Patrícia Lopes, Lídia Gomes, Luís Madeira de Carvalho","doi":"10.3390/microorganisms13092146","DOIUrl":"10.3390/microorganisms13092146","url":null,"abstract":"<p><p>Few studies have investigated gastrointestinal (GI) and external parasites, as well as environmental fungi, in rabbits using a One Health approach. Between September 2023-May 2024, fecal, hair and skin scraping samples were collected from 72 rabbits that attended clinical consultations and from private owners and breeders in Portugal. Diagnostic techniques included Mini-FLOTAC, direct immunofluorescence antibody, and the analysis of the virulence profile of fur fungi. A total of 58% of the rabbits were positive for GI parasites, namely <i>Eimeria</i> spp. (45%), <i>Cryptosporidium</i> spp. (32%), <i>Trichostrongylus retortaeformis</i> (17%), <i>Passalurus ambiguus</i> (13%), <i>Graphidium strigosum</i> (13%), and <i>Giardia</i> spp. (9%), with only 12% of the infected animals showing clinical signs (diarrhea). In addition, 10% of the animals were positive for <i>Cheyletiella</i> sp. infestations. Environmental fungi of the genera <i>Penicillium</i>, <i>Rhizopus</i>, and <i>Scopulariopsis</i> were isolated from 7% of these animals, with the <i>Scopulariopsis</i> sp. isolate S1 testing positive for proteinase, lecithinase, and gelatinase activities. Frequent sanitization and regular deworming emerged as essential factors to minimize parasitic frequency. This integrated diagnosis procedure proved to be effective in the search for parasitic and fungal agents in rabbit medicine. Further research is needed to improve the knowledge on the transmission and pathogenicity of these agents in rabbits.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Adeno-Associate Virus (AAV) Serotype for Endometriosis Therapy and Effect of AAV-Mediated RNAi Delivery on Gene Expression and Cell Proliferation in In Vitro Endometrial Cell Culture.","authors":"Jin Kyung Baek, Jaekyung Lee, Yun Soo Chung, Seokkyo Seo","doi":"10.3390/microorganisms13092144","DOIUrl":"10.3390/microorganisms13092144","url":null,"abstract":"<p><p>Endometriosis is a chronic estrogen-dependent condition with limited treatment options, often requiring surgery and long-term hormonal therapy that may impair ovarian function. Despite advancements in gene therapy for other diseases, its application in endometriosis remains largely unexplored. This study aimed to evaluate the potential of adeno-associated virus (AAV) vectors for targeted gene therapy in endometriosis. We screened multiple AAV serotypes for infectivity in primary human ectopic and eutopic endometrial cells as well as normal ovarian stromal cells. AAV serotype 3 (AAV3) demonstrated selective infectivity toward endometrial cells while sparing ovarian tissue. AAV3-mediated delivery of small interfering RNA targeting estrogen receptor 2 reduced Estrogen receptor beta (ERβ) expression to 27% in ectopic and 49% in eutopic cells. Under estradiol and inflammatory stimulation, ERβ knockdown led to modest reductions in cellular metabolic activity in eutopic cells, whereas effects in ectopic cells did not reach statistical significance. Dual targeting of ERβ and prostaglandin-endoperoxide synthase 2 (PTGS2) showed numerically lower metabolic activity than controls under some conditions but without consistent statistical significances. These findings suggest that AAV3 can serve as an ovary-sparing, endometriosis-specific vector that facilitates gene silencing while yielding limited phenotypic effects. This gene delivery system may provide a basis for developing future gene-based therapies for endometriosis.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compassionate Use of Encapsulated MKB-01 Fecal Microbiota Transplantation for Recurrent <i>Clostridioides difficile</i> Infection: A Single-Center Experience.","authors":"Ángela Cano, Elisa Ruiz Arabi, Lourdes Ruiz, Borja José Nadales, Andrés Baumela, Manuel Recio, Isabel Machuca, Juan José Castón, Elena Pérez-Nadales, Julian Torre Cisneros","doi":"10.3390/microorganisms13092134","DOIUrl":"10.3390/microorganisms13092134","url":null,"abstract":"<p><p>Fecal microbiota transplantation (FMT) is a safe and effective treatment for recurrent <i>Clostridiodes difficile</i> infection (rCDI). However, experience with the oral biologic product MKB-01 remains limited. We describe a series of 13 patients with rCDI treated with FMT using MKB-01 capsules administered orally. Each patient received a single dose of 4 capsules (≥2.1-2.5 × 10¹¹ microorganisms) with water after a 2 h fasting period. Antibiotic therapy was discontinued pre FMT. Clinical evaluation was performed at weeks 8 and 12. The mean number of prior recurrences was 1.5 (range: 1-3 episodes). In 12 patients (92.3%), FMT was administered after resolution of the current episode; in one patient (7%), it was administered on day 3 of fidaxomicin therapy, prior to symptom resolution. At week 8, clinical cure (Absence of baseline symptoms for at least 72 h) was achieved in 11 patients (84.6%). An additional patient (7%) responded to a second FMT. One recurrence occurred at 8 weeks and was resolved with a second FMT. Therefore, the overall clinical response rate after one or more FMTs was 12 out of 13 patients (92.3%). The procedure was well tolerated; only one patient experienced self-limited diarrhea. These findings support oral FMT with MKB-01 capsules as a safe and effective option for treating rCDI.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Two Lateral Flow Immunochromatographic Assays for Rapid Detection of KPC, NDM, IMP, VIM and OXA-48 Carbapenemases in Gram-Negatives.","authors":"Clara Morales Dominguez, Saoussen Oueslati, Nahed Al Laham, Réva Nermont, Hervé Volland, Thierry Naas","doi":"10.3390/microorganisms13092140","DOIUrl":"10.3390/microorganisms13092140","url":null,"abstract":"<p><p>The spread of carbapenemase-producing Gram-negative bacteria poses a significant clinical challenge due to their association with severe Difficult-to-Treat nosocomial infections, as available therapies are drastically reduced. Rapid and accurate detection of carbapenemase-producing Gram-negative bacteria is critical for effective patient management, guiding appropriate antibiotic therapy, and implementing infection control measures to limit their dissemination within healthcare settings. Lateral flow immunoassays that detect the five main carbapenemases have become cornerstones in the fight against carbapenemase-producing Gram-negative bacteria. Carbapenemases evolve in response to antibiotic exposure, and therefore regular evaluation of these lateral flow immunoassays is crucial. Here, we have evaluated a novel assay, the KINVO assay (Medomics Medical Technology) and compared it to the Gold Standard of LFIAs for carbapenemase detection, the NG-TEST CARBA 5 assay (NG-Biotech) on a large panel of carbapenemase variants. The comparison between the two assays highlighted that both share key advantages such as rapidity and simplicity. However, NG-Test CARBA 5 demonstrated superior performance overall, particularly in accurately detecting IMP-type carbapenemases and the OXA-48 variant OXA-505. In contrast, the KINVO assay was more effective at detecting a broader range of KPC variants, including some that have lost carbapenem-hydrolyzing activity but gained resistance to ceftazidime/avibactam. If we consider these variants no longer as carbapenemases, and thus that they should not be detected, the NG-Test CARBA 5 performed better for KPC carbapenemase detection.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}