马尾松人工林根际微生物群落网络特征对间伐强度的早期响应

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Size Liu, Haifeng Yin, Yu Su, Xianwei Li, Chuan Fan
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引用次数: 0

摘要

根际微生物介导植物根与土壤之间的物质交换和化学循环。然而,根际微生物群落对间伐的响应机制,特别是其共生模式,仍然知之甚少。研究了不同间伐强度下马尾松根际微生物群落,包括对照(CK, 0%)、光强间伐(LIT, 10%)、中强间伐(MIT, 30%)和高强度间伐(HIT, 50%)。通过高通量测序获得基本的分类信息,并利用R软件鉴定薄敏感操作分类单元(tsOTUs)、构建共现网络等进行统计分析。虽然α-多样性的变化规律不明显,但Kruskal-Wallis检验表明,季节是影响α-多样性变化的主要因素。间伐强度显著影响根际微生物群落结构,每种间伐强度都有特定的tsOTUs亚群。虽然夏季和冬季元共现网络的前3个模块呈现一致的tsOTU组成,但冬季引发了网络连通性的变化。无论夏季还是冬季,MIT下的网络节点数量都是最多的。此外,经过间伐后,大部分关键类群的相对丰度都有所下降;然而,MIT促进了某些关键分类群的丰富。总的来说,变薄深刻地塑造了微生物群落组成和网络特征。中等间伐强度可能是马尾松人工林的最佳间伐强度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Early Response of Rhizosphere Microbial Community Network Characteristics to Thinning Intensity in Pinus massoniana Plantations.

Rhizosphere microorganisms mediate the material exchange and chemical cycling between plant roots and soil. However, the response mechanisms of the rhizosphere microbial community, especially its co-occurrence patterns, to thinning remain poorly understood. We investigated the rhizosphere microbial communities of Pinus massoniana under different thinning intensities, including control (CK, 0%), light-intensity thinning (LIT, 10%), moderate-intensity thinning (MIT, 30%), and high-intensity thinning (HIT, 50%). Basic taxonomic information was obtained through high-throughput sequencing, while R software was utilized to identify thinning-sensitive operational taxonomic units (tsOTUs), construct co-occurrence networks, and perform other statistical analyses. Although no discernible patterns were observed in α-diversity changes, the Kruskal-Wallis test indicated that season was the primary factor driving α-diversity variation. Meanwhile, thinning intensity significantly shaped the rhizosphere microbial community structures, with each intensity harboring a specific tsOTUs subset. Although the top three modules of the meta-co-occurrence networks in summer and winter exhibited consistent tsOTU composition, winter triggered changes in network connectivity. Regardless of summer or winter, the number of network nodes under MIT was the highest. Additionally, after thinning, the relative abundances of most keystone taxa declined; however, MIT facilitated the enrichment of certain keystone taxa. Collectively, thinning profoundly shapes microbial community composition and network characteristics. Moderate thinning intensity may represent the optimal thinning intensity for the studied P. massoniana plantations.

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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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