诊断m痘的qPCR检测中Ct值的差异:一项横断面研究的结果。

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Clara Esperanza Santacruz Tinoco, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Julio Elias Alvarado Yaah, Bernardo Martínez Miguel, Yu-Mei Anguiano Hernández, Alfonso Vallejos Parás, José Esteban Muñoz Medina, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Yadira Pérez Andrade
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引用次数: 0

摘要

很少有证据表明Ct值是否受到年龄、护理类型以及从症状出现日期到获取样本的时间的影响。本研究的目的是确定年龄、护理类型和采样机会在m痘qPCR Ct值上的差异。这项描述性、回顾性研究分析了m痘的qPCR Ct值。低Ct值的几率根据年龄组、护理类型、取样机会和渗出物类型进行评估。为了确定低Ct值的几率,对变量进行了多变量双变量分析,并建立了逻辑回归模型。共查明520例m痘试验呈阳性;一般中位Ct值为23.67。住院患者与门诊患者Ct值存在差异。咽部分泌物的Ct值最高(34.24),脓疱和结痂分泌物的Ct值最低。低Ct值的样本(OR = 2.45, 95% CI: 1.13-5.29)与医院护理相关。低Ct值更可能来自在医院接受护理的个体和皮肤脓疱病变的样本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differences in Ct Values in qPCR Tests for the Diagnosis of Mpox: Results of a Cross-Sectional Study.

There is little evidence regarding whether Ct values are influenced by age, type of care, and the timing from the date of symptom onset to obtaining samples. The aim of this study was to determine the differences in the qPCR Ct values for mpox by age, type of care, and sampling opportunity. This descriptive, retrospective study analyzed the qPCR Ct values for mpox. The odds of a low Ct value were assessed by age group, type of care, sampling opportunity, and type of exudate. To determine the odds of a low Ct value, a multivariate bivariate analysis of the variables was performed, and a logistic regression model was developed. A total of 520 positive mpox tests were identified; the general median Ct value was 23.67. There was a difference in Ct values between inpatients and outpatients. Pharyngeal exudate samples had the highest Ct value (34.24), and pustule and scab exudate samples had the lowest values. Samples with a low Ct value (OR = 2.45, 95% CI: 1.13-5.29) were associated with hospital care. Low Ct values were more likely for samples from individuals being cared for in a hospital setting and with pustule skin lesions.

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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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