Clara Esperanza Santacruz Tinoco, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Julio Elias Alvarado Yaah, Bernardo Martínez Miguel, Yu-Mei Anguiano Hernández, Alfonso Vallejos Parás, José Esteban Muñoz Medina, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Yadira Pérez Andrade
{"title":"诊断m痘的qPCR检测中Ct值的差异:一项横断面研究的结果。","authors":"Clara Esperanza Santacruz Tinoco, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Julio Elias Alvarado Yaah, Bernardo Martínez Miguel, Yu-Mei Anguiano Hernández, Alfonso Vallejos Parás, José Esteban Muñoz Medina, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Yadira Pérez Andrade","doi":"10.3390/microorganisms13061355","DOIUrl":null,"url":null,"abstract":"<p><p>There is little evidence regarding whether Ct values are influenced by age, type of care, and the timing from the date of symptom onset to obtaining samples. The aim of this study was to determine the differences in the qPCR Ct values for mpox by age, type of care, and sampling opportunity. This descriptive, retrospective study analyzed the qPCR Ct values for mpox. The odds of a low Ct value were assessed by age group, type of care, sampling opportunity, and type of exudate. To determine the odds of a low Ct value, a multivariate bivariate analysis of the variables was performed, and a logistic regression model was developed. A total of 520 positive mpox tests were identified; the general median Ct value was 23.67. There was a difference in Ct values between inpatients and outpatients. Pharyngeal exudate samples had the highest Ct value (34.24), and pustule and scab exudate samples had the lowest values. Samples with a low Ct value (OR = 2.45, 95% CI: 1.13-5.29) were associated with hospital care. Low Ct values were more likely for samples from individuals being cared for in a hospital setting and with pustule skin lesions.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 6","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196091/pdf/","citationCount":"0","resultStr":"{\"title\":\"Differences in Ct Values in qPCR Tests for the Diagnosis of Mpox: Results of a Cross-Sectional Study.\",\"authors\":\"Clara Esperanza Santacruz Tinoco, Porfirio Felipe Hernández Bautista, David Alejandro Cabrera Gaytán, Julio Elias Alvarado Yaah, Bernardo Martínez Miguel, Yu-Mei Anguiano Hernández, Alfonso Vallejos Parás, José Esteban Muñoz Medina, Lumumba Arriaga Nieto, Leticia Jaimes Betancourt, Yadira Pérez Andrade\",\"doi\":\"10.3390/microorganisms13061355\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>There is little evidence regarding whether Ct values are influenced by age, type of care, and the timing from the date of symptom onset to obtaining samples. The aim of this study was to determine the differences in the qPCR Ct values for mpox by age, type of care, and sampling opportunity. This descriptive, retrospective study analyzed the qPCR Ct values for mpox. The odds of a low Ct value were assessed by age group, type of care, sampling opportunity, and type of exudate. To determine the odds of a low Ct value, a multivariate bivariate analysis of the variables was performed, and a logistic regression model was developed. A total of 520 positive mpox tests were identified; the general median Ct value was 23.67. There was a difference in Ct values between inpatients and outpatients. Pharyngeal exudate samples had the highest Ct value (34.24), and pustule and scab exudate samples had the lowest values. Samples with a low Ct value (OR = 2.45, 95% CI: 1.13-5.29) were associated with hospital care. Low Ct values were more likely for samples from individuals being cared for in a hospital setting and with pustule skin lesions.</p>\",\"PeriodicalId\":18667,\"journal\":{\"name\":\"Microorganisms\",\"volume\":\"13 6\",\"pages\":\"\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12196091/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microorganisms\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/microorganisms13061355\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms13061355","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Differences in Ct Values in qPCR Tests for the Diagnosis of Mpox: Results of a Cross-Sectional Study.
There is little evidence regarding whether Ct values are influenced by age, type of care, and the timing from the date of symptom onset to obtaining samples. The aim of this study was to determine the differences in the qPCR Ct values for mpox by age, type of care, and sampling opportunity. This descriptive, retrospective study analyzed the qPCR Ct values for mpox. The odds of a low Ct value were assessed by age group, type of care, sampling opportunity, and type of exudate. To determine the odds of a low Ct value, a multivariate bivariate analysis of the variables was performed, and a logistic regression model was developed. A total of 520 positive mpox tests were identified; the general median Ct value was 23.67. There was a difference in Ct values between inpatients and outpatients. Pharyngeal exudate samples had the highest Ct value (34.24), and pustule and scab exudate samples had the lowest values. Samples with a low Ct value (OR = 2.45, 95% CI: 1.13-5.29) were associated with hospital care. Low Ct values were more likely for samples from individuals being cared for in a hospital setting and with pustule skin lesions.
期刊介绍:
Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.