Microorganisms最新文献_第9页

Assessment of Antimicrobial Activity and Safety of Pediococcus pentosaceus Isolated from Ginseng as a Functional Cosmetic Ingredient. 人参功能性化妆品原料戊糖Pediococcus的抗菌活性及安全性评价。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051093

Xiangji Jin, Qiwen Zheng, Trang Thi Minh Nguyen, Gyeong-Seon Yi, Su-Jin Yang, Tae-Hoo Yi

{"title":"Assessment of Antimicrobial Activity and Safety of Pediococcus pentosaceus Isolated from Ginseng as a Functional Cosmetic Ingredient.","authors":"Xiangji Jin, Qiwen Zheng, Trang Thi Minh Nguyen, Gyeong-Seon Yi, Su-Jin Yang, Tae-Hoo Yi","doi":"10.3390/microorganisms13051093","DOIUrl":"https://doi.org/10.3390/microorganisms13051093","url":null,"abstract":"Lactic acid bacteria (LAB) are gaining increasing attention as functional ingredients in the cosmetic industry, particularly those derived from natural plant sources. Although various LAB strains have been widely applied in cosmetic formulations, studies investigating the effects of naturally derived LAB on the skin remain limited. In this study, we isolated an LAB strain from ginseng and evaluated its potential as a functional cosmetic ingredient. The antimicrobial activity of the strain was assessed against skin-associated pathogens Staphylococcus aureus and Staphylococcus epidermidis, while cytotoxicity was evaluated using HaCaT and Caco-2 cells. Considering the limitations of vertebrate animal testing, infection and survival assays were conducted using Galleria mellonella larvae as an alternative in vivo model. The ginseng-derived strain exhibited 99.93% similarity to Pediococcus pentosaceus and was designated P. pentosaceus THG-219. It exhibited an MIC of 0.625 mg/mL and 1.25 mg/mL against S. aureus KCTC 3881 and S. epidermidis KCTC 1917, respectively. Its antimicrobial activity was further enhanced following ethyl acetate fractionation. P. pentosaceus THG-219 showed no toxicity in G. mellonella larvae and exerted antibacterial effects in this model. No cytotoxicity was observed in HaCaT and Caco-2 cells. Furthermore, P. pentosaceus THG-219 promoted host cell adhesion while inhibiting pathogen adhesion. It also exhibited excellent acid, bile, and heat tolerance, suggesting strong survivability under harsh conditions. Collectively, these results indicate that P. pentosaceus THG-219, isolated from ginseng, is a promising, safe, and stable candidate for development as a functional cosmetic ingredient.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of the Microbial Community Profiles of Sichuan and Guizhou Smoke-Cured Sausages Using a High-Throughput Sequencing Approach. 四川和贵州烟熏香肠微生物群落特征的高通量测序比较分析

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051096

Xiangyong Zeng, Chaoyang Wei, Dounan Li, Wentao Cao, Qiang Lin

{"title":"Comparative Analysis of the Microbial Community Profiles of Sichuan and Guizhou Smoke-Cured Sausages Using a High-Throughput Sequencing Approach.","authors":"Xiangyong Zeng, Chaoyang Wei, Dounan Li, Wentao Cao, Qiang Lin","doi":"10.3390/microorganisms13051096","DOIUrl":"https://doi.org/10.3390/microorganisms13051096","url":null,"abstract":"Autochthonous microorganisms play critical roles in shaping the quality of Chinese sausages and may be influenced by local climate and/or processing conditions. The present study aimed to reveal the interprovincial differences in microbial community between Sichuan and Guizhou sausages, as well as driving factors based on high-throughput sequencing and bioinformatic analysis. The results indicated that Cobetia, Debaryomycetaceae, Kurtzmaniella, and Candida zeylanoides served as biomarkers for Sichuan sausages. In contrast, Enterococcus, unclassified Cyanobacteriales, Lactobacillales, Aspergillus vitricola, Mortierella, Fusarium, and Penicillium were identified as biomarkers for Guizhou sausages. Furthermore, salt content and moisture level showed positive correlations with Cobetia, Staphylococcus, Debaryomyces, and Kurtzmaniella, mainly found in Sichuan sausages. Conversely, pH and water activity (Aw) were positively associated with potential pathogenic bacteria (e.g., Vibrio, Cyanobacteria, Enterococcus, and Aeromonas) and fungi (e.g., Aspergillus, Fusarium, and Penicillium), which were mainly distributed in Guizhou sausages. Notably, microbial composition discrepancies between Sichuan and Guizhou sausages were primarily driven by processing conditions rather than regional climate factors. Collectively, these findings provide valuable insight for developing novel specific starters.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of SARS-CoV-2 P.1 Variant Infection on the Nasopharyngeal Commensal Bacterial Microbiome of Individuals from the Brazilian Amazon. SARS-CoV-2 P.1变异感染对巴西亚马逊地区个体鼻咽共生细菌微生物群的影响

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051088

Amanda Mendes Silva Cruz, Jedson Ferreira Cardoso, Kenny Costa Pinheiro, Jessylene Almeida Ferreira, Luana Soares Barbagelata, Sandro Patroca Silva, Wanderley Dias Chagas Junior, Patrícia Santos Lobo, Dielle Monteiro Teixeira, Walter André Junior, Inaiah Ordenes Silva, Mirleide Cordeiro Santos, Luana Silva Soares Farias, Maisa Silva Sousa, Fernando Neto Tavares

{"title":"Impact of SARS-CoV-2 P.1 Variant Infection on the Nasopharyngeal Commensal Bacterial Microbiome of Individuals from the Brazilian Amazon.","authors":"Amanda Mendes Silva Cruz, Jedson Ferreira Cardoso, Kenny Costa Pinheiro, Jessylene Almeida Ferreira, Luana Soares Barbagelata, Sandro Patroca Silva, Wanderley Dias Chagas Junior, Patrícia Santos Lobo, Dielle Monteiro Teixeira, Walter André Junior, Inaiah Ordenes Silva, Mirleide Cordeiro Santos, Luana Silva Soares Farias, Maisa Silva Sousa, Fernando Neto Tavares","doi":"10.3390/microorganisms13051088","DOIUrl":"https://doi.org/10.3390/microorganisms13051088","url":null,"abstract":"It is important to understand which bacterial taxa are most abundant during SARS-CoV-2 infection and to promote mitigation strategies for conditions subsequent to infection. Nasopharyngeal swab samples were collected from patients infected with SARS-CoV-2 and their family contacts (uninfected and asymptomatic) during the outbreak of the P.1 variant of SARS-CoV-2 in Parintins, Amazonas-Brazil, in March 2021. The samples were investigated by a shotgun sequencing metagenomic approach using the NextSeq 500 Illumina® system. The samples were stratified according to the presence or absence of SARS-CoV-2, household group, sex, and age. Of the total of 63 individuals, 37 (58.73%) were positive for SARS-CoV-2 and 26 (41.27%) were negative for SARS-CoV-2 and other respiratory viruses (FLU, AdV, HBoV, HCoV, HMPV, RSV, PIV, HRV). The alpha diversity indexes Chao1, species observed, Simpson, and Inv Simpson demonstrated a significant difference (p < 0.05) in both the diversity of observed species and the abundance of some taxa between positive and negative individuals. We also observed an abundance of opportunists such as Klebsiella pneumoniae, Staphylococcus spp, and Shigella sonnei, previously associated with the severity of COVID-19. Our results suggest that SARS-CoV-2 infection causes changes in the microenvironment of the nasopharyngeal region, allowing greater proliferation of opportunistic bacteria and decreased abundance of commensal bacteria.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Laboratory Validation of Rapid, Bird-Side Molecular Diagnostic Assays for Avian Influenza Virus Including Panzootic H5Nx. 开发和实验室验证禽流感病毒快速，鸟侧分子诊断分析，包括大流行性H5Nx。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-08 DOI: 10.3390/microorganisms13051090

Matthew Coopersmith, Remco Dijkman, Maggie L Bartlett, Richard Currie, Sander Schuurman, Sjaak de Wit

{"title":"Development and Laboratory Validation of Rapid, Bird-Side Molecular Diagnostic Assays for Avian Influenza Virus Including Panzootic H5Nx.","authors":"Matthew Coopersmith, Remco Dijkman, Maggie L Bartlett, Richard Currie, Sander Schuurman, Sjaak de Wit","doi":"10.3390/microorganisms13051090","DOIUrl":"https://doi.org/10.3390/microorganisms13051090","url":null,"abstract":"Avian influenza A viruses (AIV) significantly impact both animal and human health. Reliable diagnostics are crucial for controlling AIV, including the highly pathogenic strains like H5Nx. In this study, we developed and validated the on-site Alveo Sense Poultry Avian Influenza Tests to rapidly detect the AIV M-gene and subtypes H5, H7, and H9 in unprocessed samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and impedance-based measurements. The Alveo Sense tests, using single-use microfluidic cartridges, deliver results within 45 min. Each cartridge includes assays for the AIV M gene and specific H5 and H7 or H9 subtypes, with internal process controls. The laboratory validation involved specificity, limit of detection (LoD), diagnostic sensitivity, reproducibility, and robustness tests using various AIV strains, other avian pathogens, and field samples. The assays showed 100% specificity for AIV subtypes without cross-reactivity with non-AIV pathogens. The LoD95 for H5, H7, and H9 ranged between RT-PCR Ct values of 29-33 in both cloacal and oropharyngeal samples and were able to detect avian influenza virus in both spiked samples and field samples. Reproducibility and repeatability studies showed perfect agreement across operators and laboratories and remained stable and accurate under different pre-analytical conditions. The Alveo Sense tests offer rapid, accurate, and reliable on-site diagnostics for AIV subtypes H5, H7, and H9 on samples from fresh dead and sick birds, valuable for early flock-level detection and outbreak control. Further field validation will improve the understanding of their diagnostic performance across various avian species.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Basis of Gap Formation Between Migrating Helicobacter pylori Colonies in Soft Agar Assays. 软琼脂试验中幽门螺杆菌菌落迁移间隙形成的遗传基础。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-07 DOI: 10.3390/microorganisms13051087

Yasmine Elshenawi, Skander Hathroubi, Shuai Hu, Xiaolin Liu, Karen M Ottemann

{"title":"Genetic Basis of Gap Formation Between Migrating Helicobacter pylori Colonies in Soft Agar Assays.","authors":"Yasmine Elshenawi, Skander Hathroubi, Shuai Hu, Xiaolin Liu, Karen M Ottemann","doi":"10.3390/microorganisms13051087","DOIUrl":"https://doi.org/10.3390/microorganisms13051087","url":null,"abstract":"Helicobacter pylori is a motile bacterial pathogen that causes severe gastric diseases. H. pylori motility and chemotaxis are key colonization factors. Motility and chemotaxis are studied in many microbes, including H. pylori, using soft agar assays. In these assays, bacteria are inoculated into low-percentage agar and expand in a motility- and chemotaxis-dependent manner. H. pylori similarly expands in soft agar, but, if a plate was inoculated at multiple points, the expanded H. pylori colonies did not merge and left gaps. The basis of these gaps was unknown. We report here that gap formation was not affected by media components such as nutrient and agar concentrations, nor did it require chemotaxis, but it did rely on quorum sensing. To broaden our understanding of this H. pylori property, an H. pylori Tn7 transposon library was screened for mutants that lost gap formation. Fourteen mutants were identified, with transposon sites mapped to genes encoding outer membrane proteins, cysteine-rich proteins, phosphatidyl glycerophosphate synthase, an endorestriction nuclease, and several hypothetical proteins. Our results suggest that H. pylori may use specific proteins to avoid contact with other H. pylori, a behavior that may relate to previous observations that different H. pylori strains do not mix populations in stomach glands.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut Microbiota: An Immersion in Dysbiosis, Associated Pathologies, and Probiotics. 肠道微生物群：沉浸在生态失调、相关病理和益生菌中。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-07 DOI: 10.3390/microorganisms13051084

Valentina Origüela, Alvaro Lopez-Zaplana

引用次数: 0

Probiotic Administration Contributes to the Improvement in Intestinal Dysregulation Induced by Allergic Contact Dermatitis. 给予益生菌有助于改善过敏性接触性皮炎引起的肠道失调。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-07 DOI: 10.3390/microorganisms13051082

Eduardo Mendes, Evelyn Roxana Perez Umana, Daniel Di Pace Soares Penna, Fernando Augusto de Oliveira, Leandro Nascimento Lemos, Willian Rodrigues Ribeiro, Mateus Barbosa Casaro, Mariana Lazarini, Valéria Maia Oliveira, Caroline Marcantonio Ferreira

{"title":"Probiotic Administration Contributes to the Improvement in Intestinal Dysregulation Induced by Allergic Contact Dermatitis.","authors":"Eduardo Mendes, Evelyn Roxana Perez Umana, Daniel Di Pace Soares Penna, Fernando Augusto de Oliveira, Leandro Nascimento Lemos, Willian Rodrigues Ribeiro, Mateus Barbosa Casaro, Mariana Lazarini, Valéria Maia Oliveira, Caroline Marcantonio Ferreira","doi":"10.3390/microorganisms13051082","DOIUrl":"https://doi.org/10.3390/microorganisms13051082","url":null,"abstract":"Recent studies have emphasized the impact of gut microbiota on skin health, but the reverse, how skin diseases affect gut homeostasis, has received less attention. Allergic contact dermatitis (ACD), a common skin disorder affecting one in four people worldwide, can be accompanied by intestinal disturbances. To explore this, we used an experimental model of ACD to investigate the intestinal changes induced by the disease. Parameters assessed included intestinal microbiota, short-chain fatty acids (SCFAs), gene expression related to intestinal permeability, inflammatory cytokines, and mucus production. To evaluate potential therapeutic interventions, the probiotic Bifidobacterium longum strain BB536 was administered via gavage, starting 10 days before dermatitis induction and continuing until the last day of disease induction. ACD caused alterations in the composition of intestinal microbiota compared to naïve mice but did not affect SCFA production. The probiotic altered microbiota composition and increased acetate production in dermatitis-induced mice. ACD decreased the gene expression of TjP1, ATHO1, and MUC2, while probiotic treatment restored TjP1 and ATHO1 to normal levels. The cytokine IL-6 increased in the ACD group compared to naïve mice, whereas IL-10 decreased; probiotic treatment also restored these levels. Intestinal mucus production, affected by ACD, was partially restored by probiotic treatment. The findings suggest that probiotics could be a therapeutic strategy to prevent intestinal issues caused by skin diseases.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Novel 2018 Influenza Virus Outbreak on the Yucatan Peninsula, Mexico, in the Summer. 墨西哥尤卡坦半岛2018年夏季新型流感病毒爆发特征分析

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-07 DOI: 10.3390/microorganisms13051086

Lumumba Arriaga-Nieto, David Alejandro Cabrera-Gaytán, Alfonso Vallejos-Parás, Porfirio Felipe Hernández-Bautista, Clara Esperanza Santacruz-Tinoco, Julio Elías Alvarado-Yaah, Yu-Mei Anguiano-Hernández, Bernardo Martínez-Miguel, María Erandhí Prieto-Torres, Concepción Grajales-Muñiz, Nancy Sandoval-Gutiérrez, Horacia Celina Velarde-Scull

{"title":"Characterization of a Novel 2018 Influenza Virus Outbreak on the Yucatan Peninsula, Mexico, in the Summer.","authors":"Lumumba Arriaga-Nieto, David Alejandro Cabrera-Gaytán, Alfonso Vallejos-Parás, Porfirio Felipe Hernández-Bautista, Clara Esperanza Santacruz-Tinoco, Julio Elías Alvarado-Yaah, Yu-Mei Anguiano-Hernández, Bernardo Martínez-Miguel, María Erandhí Prieto-Torres, Concepción Grajales-Muñiz, Nancy Sandoval-Gutiérrez, Horacia Celina Velarde-Scull","doi":"10.3390/microorganisms13051086","DOIUrl":"https://doi.org/10.3390/microorganisms13051086","url":null,"abstract":"During the 2017-2018 influenza season, there was high influenza activity, with a predominance of influenza A(H1N1)pdm09 circulation in the country. The influenza circulation pattern in the area of the Yucatan Peninsula was different from that of the rest of the country. However, in the summer of 2018, there was a sudden increase in the number of identified cases. A retrospective analysis was performed using data generated by four molecular diagnostic laboratories of the Mexican Social Security Institute. Demographics, influenza positivity, seasonality and case fatality rates were recorded. We used odds ratios to compare outpatients who were confirmed by laboratory tests to be positive with those who were confirmed to be negative. The Kaplan-Meier method and Cox multivariate analysis were used to calculate cumulative risk. There were 4460 cases of ILI/SARI between Yucatan and Quintana Roo, which represented 53.1% of the total number of cases reported. Compared with that in 2009, the epidemic wave in 2018 was shorter and more expansive, with a greater number of reported cases, as well as a greater number of people who required hospitalization. The dominant pattern of A(H1N1)pdm09 influenza activity on the Yucatan Peninsula in the summer of 2018 has not been observed since the influenza pandemic of 2009.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival of Filamentous Cyanobacteria Through Martian ISRU: Combined Effects of Desiccation and UV-B Radiation. 丝状蓝藻在火星ISRU中的存活：干燥和UV-B辐射的联合影响。

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-07 DOI: 10.3390/microorganisms13051083

Miguel Arribas Tiemblo, Inês P E Macário, Antonio Tornero, Ana Yáñez, Slavka Andrejkovičová, Felipe Gómez

{"title":"Survival of Filamentous Cyanobacteria Through Martian ISRU: Combined Effects of Desiccation and UV-B Radiation.","authors":"Miguel Arribas Tiemblo, Inês P E Macário, Antonio Tornero, Ana Yáñez, Slavka Andrejkovičová, Felipe Gómez","doi":"10.3390/microorganisms13051083","DOIUrl":"https://doi.org/10.3390/microorganisms13051083","url":null,"abstract":"Cyanobacteria are a widespread group of photosynthesizing prokaryotes potentially relevant for space exploration, as they can produce both oxygen and organic matter. These organisms have been repeatedly proposed as tools for colonizing planetary bodies in the solar system. We used several Martian regolith simulants to support the growth of three widespread filamentous cyanobacteria (Desmonostoc muscorum UTAD N213, Anabaena cylindrica UTAD A212 and an uncharacterized Desmonostoc sp.). All cyanobacteria grew well on the surface of the commercial simulants MGS-1 and MMS-2 and in soluble extracts obtained from them, suggesting that these Martian regolith analogs contain everything necessary to sustain cyanobacterial growth, at least in the short term. We also evaluated the survival of the two Desmonostoc species under desiccation and UV-B radiation, using the same regolith simulants and two clays: Montmorillonite and nontronite. Desiccation hindered growth, but both cyanobacteria were able to recover in less than 30 days in all cases after desiccation. Short irradiation times (up to 1000 kJ/m2) did not consistently affect survival, but longer ones (24,000 kJ/m2) could fully sterilize some samples, although cyanobacteria within MGS-1, montmorillonite and nontronite showed signs of recovery in the long term (>70 days). Clays led to very fast recoveries, particularly montmorillonite.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Vascular Catheter Removal Versus Retention in Non-ICU Patients with CRBSI or CABSI in Retrospective, Single-Center Study. 回顾性单中心研究：非icu CRBSI或CABSI患者血管导管拔除与保留的有效性

IF 4.1 2区生物学

Microorganisms Pub Date : 2025-05-07 DOI: 10.3390/microorganisms13051085

Giovanni De Capitani, Marta Colaneri, Claudia Conflitti, Fabio Borgonovo, Lucia Galli, Giovanni Scaglione, Camilla Genovese, Rebecca Fattore, Monica Schiavini, Beatrice Caloni, Daniele Zizzo, Nicola Busatto, Antonio Gidaro, Alba Taino, Maria Calloni, Francesco Casella, Arianna Bartoli, Chiara Cogliati, Emanuele Palomba, Spinello Antinori, Andrea Gori, Antonella Foschi

{"title":"Effectiveness of Vascular Catheter Removal Versus Retention in Non-ICU Patients with CRBSI or CABSI in Retrospective, Single-Center Study.","authors":"Giovanni De Capitani, Marta Colaneri, Claudia Conflitti, Fabio Borgonovo, Lucia Galli, Giovanni Scaglione, Camilla Genovese, Rebecca Fattore, Monica Schiavini, Beatrice Caloni, Daniele Zizzo, Nicola Busatto, Antonio Gidaro, Alba Taino, Maria Calloni, Francesco Casella, Arianna Bartoli, Chiara Cogliati, Emanuele Palomba, Spinello Antinori, Andrea Gori, Antonella Foschi","doi":"10.3390/microorganisms13051085","DOIUrl":"https://doi.org/10.3390/microorganisms13051085","url":null,"abstract":"Catheter-associated bloodstream infections (CABSIs) and catheter-related bloodstream infections (CRBSIs) are significant causes of morbidity and mortality worldwide. The current practice favors the removal of vascular access devices (VADs); however, the evidence on this topic remains inconclusive. This study evaluates the clinical outcomes in terms of in-hospital mortality and catheter retention vs. removal in CABSI and CRBSI cases. A retrospective, observational, single-center study was conducted at Luigi Sacco Hospital, Milan, Italy (May 2021-December 2023), and it analyzed non-ICU adult patients with VADs diagnosed with CRBSIs or CABSIs. Clinical and microbiological data were collected to assess the outcomes based on catheter management. Among 1874 patients with VADs, 147 were included, with 164 VAD infection events (92 CABSIs and 72 CRBSIs). Overall, 35 (23.8%) patients with CABSIs and CRBSIs died. Out of those who retained the catheter 19 (35.8%) patients died, while among removal patients 16 (17%) died (p = 0.018). A Candida spp. isolation was found to be significantly associated with a higher likelihood of catheter removal (p = 0.04). Our findings suggest that, in non-ICU CRBSI and CABSI cases, VAD removal may be associated with improved outcomes when feasible.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 5","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0