Microorganisms最新文献_第9页

Oxford Nanopore Technology-Based Identification of an Acanthamoeba castellanii Endosymbiosis in Microbial Keratitis. 基于牛津纳米孔技术鉴定微生物角膜炎中的棘阿米巴内共生。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112292

Sebastian Alexander Scharf, Lennart Friedrichs, Robert Bock, Maria Borrelli, Colin MacKenzie, Klaus Pfeffer, Birgit Henrich

{"title":"Oxford Nanopore Technology-Based Identification of an Acanthamoeba castellanii Endosymbiosis in Microbial Keratitis.","authors":"Sebastian Alexander Scharf, Lennart Friedrichs, Robert Bock, Maria Borrelli, Colin MacKenzie, Klaus Pfeffer, Birgit Henrich","doi":"10.3390/microorganisms12112292","DOIUrl":"https://doi.org/10.3390/microorganisms12112292","url":null,"abstract":"(1) Background: Microbial keratitis is a serious eye infection that carries a significant risk of vision loss. Acanthamoeba spp. are known to cause keratitis and their bacterial endosymbionts can increase virulence and/or treatment resistance and thus significantly worsen the course of the disease. (2) Methods and Results: In a suspected case of Acanthamoeba keratitis, in addition to Acanthamoeba spp., an endosymbiont of acanthamoebae belonging to the taxonomic order of Holosporales was detected by chance in a bacterial 16S rDNA-based pan-PCR and subsequently classified as Candidatus Paracaedibacter symbiosus through an analysis of an enlarged 16S rDNA region. We used Oxford Nanopore Technology to evaluate the usefulness of whole-genome sequencing (WGS) as a one-step diagnostics method. Here, Acanthamoeba castellanii and the endosymbiont Candidatus Paracaedibacter symbiosus could be directly detected at the species level. No other microbes were identified in the specimen. (3) Conclusions: We recommend the introduction of WGS as a diagnostic approach for keratitis to replace the need for multiple species-specific qPCRs in future routine diagnostics and to enable an all-encompassing characterisation of the polymicrobial community in one step.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H9 Consensus Hemagglutinin Subunit Vaccine with Adjuvants Induces Robust Mucosal and Systemic Immune Responses in Mice by Intranasal Administration. 含佐剂的 H9 共识血凝素亚单位疫苗通过鼻内给药诱导小鼠产生强大的粘膜和全身免疫反应。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112294

Liming Lin, Shunfan Zhu, Beibei Yang, Xin Zhang, Huimin Wu, Shixiang Wu, Li Wu, Jianhong Shu, Yulong He, Huapeng Feng

{"title":"H9 Consensus Hemagglutinin Subunit Vaccine with Adjuvants Induces Robust Mucosal and Systemic Immune Responses in Mice by Intranasal Administration.","authors":"Liming Lin, Shunfan Zhu, Beibei Yang, Xin Zhang, Huimin Wu, Shixiang Wu, Li Wu, Jianhong Shu, Yulong He, Huapeng Feng","doi":"10.3390/microorganisms12112294","DOIUrl":"https://doi.org/10.3390/microorganisms12112294","url":null,"abstract":"The H9N2 subtype avian influenza viruses mainly cause respiratory symptoms, reduce the egg production and fertility of poultry, and result in secondary infections, posing a great threat to the poultry industry and human health. Currently, all H9N2 avian influenza commercial vaccines are inactivated vaccines, which provide protection for immunized animals but cannot inhibit the spread of the virus and make it difficult to distinguish between the infected animals and vaccinated animals. In this study, a trimeric consensus H9 hemagglutinin (HA) subunit vaccine for the H9N2 subtype avian influenza virus based on a baculovirus expression system was first generated, and then the effects of three molecular adjuvants on the H9 HA subunit vaccine, Cholera toxin subunit B (CTB), flagellin, and granulocyte-macrophage colony-stimulating factor (GM-CSF) fused with H9 HA, and one synthetic compound, a polyinosinic-polycytidylic acid (PolyI:C) adjuvant, were evaluated in mice by intranasal administration. The results showed that these four adjuvants enhanced the immunogenicity of the H9 HA subunit vaccine for avian influenza viruses, and that GM-CSF and PolyI:C present better mucosal adjuvant activity for the H9 HA subunit vaccine. These results demonstrate that we have developed a potential universal H9 HA mucosal subunit vaccine with adjuvants in a baculovirus system that would be helpful for the prevention and control of H9N2 subtype avian influenza viruses.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accessing Fungal Contributions to the Birch Effect: Real-Time Respiration from Pore-Scale Microfluidics. 获取真菌对桦树效应的贡献：从孔隙级微流控技术中获得实时呼吸作用

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112295

Yi-Syuan Guo, Karl K Weitz, Aramy Truong, Adam G Ryan, Leslie M Shor, Arunima Bhattacharjee, Mary S Lipton

{"title":"Accessing Fungal Contributions to the Birch Effect: Real-Time Respiration from Pore-Scale Microfluidics.","authors":"Yi-Syuan Guo, Karl K Weitz, Aramy Truong, Adam G Ryan, Leslie M Shor, Arunima Bhattacharjee, Mary S Lipton","doi":"10.3390/microorganisms12112295","DOIUrl":"https://doi.org/10.3390/microorganisms12112295","url":null,"abstract":"Drying and rewetting of soil stimulates soil carbon emission. The Birch effect, driven by these cycles, leads to CO2 efflux, which can be monitored using real-time mass spectrometry (RTMS). Although soil fungi retain water during droughts, their contribution to CO2 release during drying-rewetting cycles remains unclear. In this study, we present the first demonstration of integrating micromodels with RTMS to monitor the Birch effect by simulating drought and rewetting. Micromodels were inoculated with axenic fungal culture and dried to assess moisture retention. After drying, RTMS quantified CO2 release upon rewetting with H218O mixtures. Our results showed that soil fungi released CO2 upon rehydration and immediately utilized the external water source at the pore scale by generating subsequent 46CO2. This work is the first to integrate RTMS with microsystems to investigate pore-scale biogeochemistry and the involvement of fungi in the Birch effect.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic Insight into the Anti-Bacterial/Anti-Biofilm Effects of Low Chlorhexidine Concentrations on Enterococcus faecalis-In Vitro Study. 低浓度洗必泰对粪肠球菌抗菌/抗生物膜作用的机制洞察--体外研究。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112297

Nathanyel Sebbane, Itzhak Abramovitz, Nurit Kot-Limon, Doron Steinberg

{"title":"Mechanistic Insight into the Anti-Bacterial/Anti-Biofilm Effects of Low Chlorhexidine Concentrations on Enterococcus faecalis-In Vitro Study.","authors":"Nathanyel Sebbane, Itzhak Abramovitz, Nurit Kot-Limon, Doron Steinberg","doi":"10.3390/microorganisms12112297","DOIUrl":"https://doi.org/10.3390/microorganisms12112297","url":null,"abstract":"Background: Endodontic treatment failures are often linked to the persistence of Enterococcus faecalis in the root canal system. This study aimed to investigate the antibacterial/antibiofilm mechanism of chlorhexidine (CHX), particularly at low concentrations, against E. faecalis, to improve endodontic treatment protocols.Methods: The antibacterial activity of CHX (0.125-20 μg/mL) was evaluated against E. faecalis ATCC 29212 using various assays, including planktonic growth inhibition, colony-forming units (CFUs), membrane permeability and potential assays, high-resolution scanning electron microscopy (HR-SEM), confocal laser scanning microscopy of biofilms, biomass and metabolic activity assays on matured biofilm, and quantitative real-time PCR for gene expression. Statistical analysis was performed using Student's t-test and ANOVA.Results: CHX demonstrated concentration-dependent inhibition of E. faecalis, significantly reducing planktonic growth and CFUs. Membrane assays showed increased permeability and depolarization, indicating damage. HR-SEM revealed morphological changes, such as pore formation, while confocal microscopy showed a reduction in biofilm mass and extracellular substances. Gene expression analysis indicated the downregulation of virulence genes and upregulation of stress response genes.Conclusions: CHX at low concentrations disrupts E. faecalis at multiple levels, from membrane disruption to gene expression modulation, affecting mature biofilm. These findings support the refinement of endodontic disinfection protocols to reduce microbial persistence.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Bacterial Community, Core Taxa, and Functional Variations Within the Fruiting Bodies of Laccaria. Laccaria 果实体内的内生细菌群落、核心类群和功能变异。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112296

Kaixuan Zhang, Xin Chen, Xiaofei Shi, Zhenyan Yang, Lian Yang, Dong Liu, Fuqiang Yu

{"title":"Endophytic Bacterial Community, Core Taxa, and Functional Variations Within the Fruiting Bodies of Laccaria.","authors":"Kaixuan Zhang, Xin Chen, Xiaofei Shi, Zhenyan Yang, Lian Yang, Dong Liu, Fuqiang Yu","doi":"10.3390/microorganisms12112296","DOIUrl":"https://doi.org/10.3390/microorganisms12112296","url":null,"abstract":"Macrofungi do not exist in isolation but establish symbiotic relationships with microorganisms, particularly bacteria, within their fruiting bodies. Herein, we examined the fruiting bodies' bacteriome of seven species of the genus Laccaria collected from four locations in Yunnan, China. By analyzing bacterial diversity, community structure, and function through 16S rRNA sequencing, we observed the following: (1) In total, 4,840,291 high-quality bacterial sequences obtained from the fruiting bodies were grouped into 16,577 amplicon sequence variants (ASVs), and all samples comprised 23 shared bacterial ASVs. (2) The Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium complex was found to be the most abundant and presumably coexisting bacterium. (3) A network analysis revealed that endophytic bacteria formed functional groups, which were dominated by the genera Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Novosphingobium, and Variovorax. (4) The diversity, community structure, and dominance of ecological functions (chemoheterotrophy and nitrogen cycling) among endophytic bacteria were significantly shaped by geographic location, habitat, and fungal genotype, rather than fruiting body type. (5) A large number of the endophytic bacteria within Laccaria are bacteria that promote plant growth; however, some pathogenic bacteria that pose a threat to human health might also be present. This research advances our understanding of the microbial ecology of Laccaria and the factors shaping its endophytic bacterial communities.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Bioactive Metabolite Profiling of Streptomyces sp. Y009: A Mangrove-Derived Actinomycetia with Anticancer and Antioxidant Potential. 具有抗癌和抗氧化潜力的红树林放线菌--链霉菌 Y009 的特性和生物活性代谢物分析：具有抗癌和抗氧化潜力的红树林放线菌。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112300

Bo Yu, Wei Zeng, Yuting Zhou, Nan Li, Zhiqun Liang

{"title":"Characterization and Bioactive Metabolite Profiling of Streptomyces sp. Y009: A Mangrove-Derived Actinomycetia with Anticancer and Antioxidant Potential.","authors":"Bo Yu, Wei Zeng, Yuting Zhou, Nan Li, Zhiqun Liang","doi":"10.3390/microorganisms12112300","DOIUrl":"https://doi.org/10.3390/microorganisms12112300","url":null,"abstract":"Microorganisms from poorly explored environments are promising sources for the development of novel drugs. In our continuous efforts to screen for mangrove actinomycetes that produce metabolites with potential pharmaceutical applications, Streptomyces sp. Y009 was isolated from mangrove sediments in Guangxi, China. The phenotypic, physiological, biochemical, and phylogenetic characteristics of this strain were investigated. Analysis of phylogenetic and 16S rRNA gene sequences showed that it had the highest sequence similarity to Streptomyces thermolilacinus NBRC 14274 (98.95%). Further, the Y009 extract exhibited antioxidant activity, as indicated by DPPH and superoxide dismutase assays. The extract showed broad-spectrum and potent anticancer potential against six human cancer cell lines, with IC50 values ranging from 5.61 to 72.15 μg/mL. Furthermore, the selectivity index (SI) demonstrated that the Y009 extract exhibited less toxicity toward normal cell lines in comparison to the lung cancer cell line (A549) and hepatoma cell line (HepG2). GC-MS analysis revealed that the extract contained some biologically important secondary metabolites, mainly cyclic dipeptides and esters, which might be responsible for the antioxidant and anticancer properties. 3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (28.32%) was the major chemical compound available in the extract. The effect on cancer cells was then confirmed using nuclear staining and in silico docking. This study suggests that further exploration of the bioactive compounds of the newly isolated strain may be a promising approach for the development of novel chemopreventive drugs.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Randomized Clinical Trials Demonstrate the Safety Assessment of Alkalihalobacillus clausii AO1125 for Use as a Probiotic in Humans. 随机临床试验证明了将碱性苛氧杆菌 AO1125 用作人体益生菌的安全性评估。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112299

Gissel García, Josanne Soto, Antonio Díaz, Jesús Barreto, Carmen Soto, Ana Beatriz Pérez, Suselys Boffill, Raúl De Jesús Cano

{"title":"Randomized Clinical Trials Demonstrate the Safety Assessment of Alkalihalobacillus clausii AO1125 for Use as a Probiotic in Humans.","authors":"Gissel García, Josanne Soto, Antonio Díaz, Jesús Barreto, Carmen Soto, Ana Beatriz Pérez, Suselys Boffill, Raúl De Jesús Cano","doi":"10.3390/microorganisms12112299","DOIUrl":"https://doi.org/10.3390/microorganisms12112299","url":null,"abstract":"(1) Background: Alkalihalobacillus clausii AO1125 is a Gram-positive, motile, spore-forming bacterium with potential as a probiotic due to its broad-spectrum antimicrobial activity, inhibiting pathogens like Listeria monocytogenes, Staphylococcus aureus, and Clostridium difficile, as well as anti-rotavirus activity. Its resilience in gastrointestinal conditions suggests benefits for gut health. This study evaluates the safety and probiotic potential of A. clausii AO1125. (2) Methods: Genome annotation identified genes linked to probiotic traits such as stress resistance, gut colonization, immune modulation, and antimicrobial production. The genome was screened for antibiotic resistance genes using CARD, bacteriocin clusters using BAGEL4, and virulence factors via VFDB. Cytotoxicity was assessed on Vero cells and erythrocytes, and a Phase I, double-blind, placebo-controlled clinical trial was conducted with 99 healthy volunteers (50 AO1125, 49 placebo). (3) Results: Genomic analysis confirmed minimal antibiotic resistance genes and the absence of virulence factors, supporting safety. A. clausii AO1125 showed no pathogenicity, cytotoxicity, or hemolytic activity and was well-tolerated in clinical settings, with mild, transient abdominal gas as the most common adverse event. (4) Conclusions: The safety profile and genetic basis for probiotic and antimicrobial properties support A. clausii AO1125 as a promising probiotic candidate for gastrointestinal health, warranting further clinical research.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain Rhodococcus sp. DH-2. 耐重金属菌株 Rhodococcus sp. DH-2 对原油和苯胺的生物降解。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112293

Zetian Luo, Jiajun Ma, Lei Huang, Dahui Li, Guohui Gao, Yihe Zhao, Agostinho Antunes, Meitong Li

{"title":"Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain Rhodococcus sp. DH-2.","authors":"Zetian Luo, Jiajun Ma, Lei Huang, Dahui Li, Guohui Gao, Yihe Zhao, Agostinho Antunes, Meitong Li","doi":"10.3390/microorganisms12112293","DOIUrl":"https://doi.org/10.3390/microorganisms12112293","url":null,"abstract":"Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus Rhodococcus. The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC-MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Analysis of the Ocular Microbiome: Insights into Healthy Eyes and Anophthalmic Sockets. 眼部微生物组的比较分析：洞察健康眼睛和眼底病眼窝。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112298

Francisco Zamorano-Martín, Guillermo Chumaceiro, Pablo Navarro-Torres, Davide Borroni, Facundo Urbinati, Ángel Molina, Andreu Paytuví-Gallart, Carlos Rocha-de-Lossada

{"title":"A Comparative Analysis of the Ocular Microbiome: Insights into Healthy Eyes and Anophthalmic Sockets.","authors":"Francisco Zamorano-Martín, Guillermo Chumaceiro, Pablo Navarro-Torres, Davide Borroni, Facundo Urbinati, Ángel Molina, Andreu Paytuví-Gallart, Carlos Rocha-de-Lossada","doi":"10.3390/microorganisms12112298","DOIUrl":"https://doi.org/10.3390/microorganisms12112298","url":null,"abstract":"The purpose of this study is to characterize the ocular surface microbiota of patients with an anophthalmic cavity. An eNAT with 1 mL of Liquid Amies Medium was used to collect samples. Microbial DNA from anophthalmic socket and healthy fellow control eye samples was isolated and sequenced. Raw reads were analyzed with GAIA (v 2.02). The richness and Shannon alpha diversity metrics, as well as Bray-Curtis beta diversity and Wilcoxon signed-rank test values, were computed with R packages such as phyloseq, mia, or DESeq2 to allow for microbiome analysis. Principal coordinate analysis (PCoA) was performed using the function plotReducedDim from the R package scater. The different taxonomic profiles were described under the concept of eye community state type (ECST). The microbiomes of both eyes from 25 patients with an anophthalmic cavity were analyzed in this study. While the microbial communities of paired eyes from the same patients showed notable dissimilarity, no consistent patterns emerged when comparing healthy eyes to anophthalmic sockets. Alpha diversity values did not significantly differ between healthy eyes and anophthalmic socket samples, though there was considerable variability within each group. Notably, anophthalmic socket samples generally exhibited lower abundances of genera such as Staphylococcus, Enterococcus, Paenibacillus, and Sediminibacterium compared to their healthy counterparts. Microbial variability between healthy eyes and anophthalmic sockets may be due to anatomical differences. Further research is needed to determine whether patients without anophthalmic sockets exhibit similar microbiome patterns in both eyes.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of the Electrical Microbial Growth Analyzer Method for Efficiently Quantifying Viable Bacteria in Ready-to-Eat Sea Cucumber Products. 应用电微生物生长分析仪方法有效定量即食海参产品中的存活细菌。

IF 4.1 2区生物学

Microorganisms Pub Date : 2024-11-12 DOI: 10.3390/microorganisms12112301

Xiaoyang Wang, Ruohan Liang, Xiaodan Pu, Yuanyuan Zhang, Feng Lu, Qianqian Yang, Xueting Zhu, Qing Kong, Xuzhi Zhang

{"title":"Application of the Electrical Microbial Growth Analyzer Method for Efficiently Quantifying Viable Bacteria in Ready-to-Eat Sea Cucumber Products.","authors":"Xiaoyang Wang, Ruohan Liang, Xiaodan Pu, Yuanyuan Zhang, Feng Lu, Qianqian Yang, Xueting Zhu, Qing Kong, Xuzhi Zhang","doi":"10.3390/microorganisms12112301","DOIUrl":"https://doi.org/10.3390/microorganisms12112301","url":null,"abstract":"Accurate and efficient quantification of viable bacteria in ready-to-eat food products is crucial for food safety and public health. The rapid and accurate assessment of foodborne bacteria in complex food matrices remains a significant challenge. Herein a culture-based approach was established for easily quantifying viable bacteria in ready-to-eat sea cucumber (RSC) products. Samples of the liquid companion within the package were directly transferred into test tubes to determine bacterial growth curves and growth rate curves, utilizing the electrical microbial growth analyzer. Viable bacteria in the samples were then quantified based on the time required to attain the maximum growth rate indicated on the growth rate curve. At a concentration of 5.0 × 103 CFU/mL of viable bacteria in the liquid companion, the recovery rates were 108.85-112.77% for Escherichia coli (E. coli) and 107.01-130.54% for Staphylococcus aureus (S. aureus), with standard deviations of 1.60 and 3.92, respectively. For the solid content in the package, the quantification was performed using the same methodology following an additional homogenization step. At a concentration of 5.0 × 103 CFU/mL of viable bacteria in the sample, the recovery rates were 91.94-102.24% for E. coli and 81.43-104.46% for S. aureus, with standard deviations of 2.34 and 2.38, respectively. In instances where the viable bacterial concentration was 5.0 × 103 CFU/mL in RSC products, the total time required for the quantification did not exceed 10.5 h. This method demonstrated advantages over traditional plate counting and PCR methods regarding simplicity and efficiency, representing a promising alternative for the quantification of viable bacteria in food like RSC products.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 11","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0