Microorganisms最新文献_第9页

Halogenation and Dehalogenation Potential of Microorganisms in Yangtze River Waters. 长江水体微生物的卤化和去卤化潜力。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092133

Zhixuan Wang, Lin Hu, Li Wang, Rulong Liu

{"title":"Halogenation and Dehalogenation Potential of Microorganisms in Yangtze River Waters.","authors":"Zhixuan Wang, Lin Hu, Li Wang, Rulong Liu","doi":"10.3390/microorganisms13092133","DOIUrl":"10.3390/microorganisms13092133","url":null,"abstract":"The discharge of pollutants into rivers has been increasing with the rapid industrial development and extensive agricultural use of pesticides and herbicides. Halogenated organic compounds (HOCs) represent a significant class of environmental pollutants. It has been found that microorganisms have the ability not only to degrade HOCs but also to synthesize them. Little is known about the halogenation and dehalogenation potential of microorganisms in river waters. In this study, we investigated the halogenation and dehalogenation potentials of microorganisms in the Yangtze River, which originates from the Tibetan Plateau, flows through southwestern, central and eastern China, and finally joins the East China Sea. A systematic metagenomic and bioinformatics analysis identified and quantified genes encoding four dehalogenases and two halogenases, providing fundamental data for the halogen cycle in the Yangtze River water body. The study showed that the microbial community in the Yangtze water body was mainly associated with dehalogenation potential, and the relative abundance of dehalogenase genes was higher than that of halogenase genes. Among the microorganisms with halogenation and dehalogenation potentials, Pseudomonadota and Actinomycetota dominated. Some microorganisms possessed both halogenation and dehalogenation functions, suggesting a potential adaptive strategy to environmental fluctuations. The presence of diverse and complete dehalogenation metabolic pathways highlights the microbial potential for bioremediation. These microorganisms not only contribute to the degradation of halogenated organic matter but also play crucial roles in carbon, nitrogen, and sulfur cycling. This study provides essential data for understanding microbial halogenation and dehalogenation potential in the Yangtze River, offering insights into the microbial-driven biogeochemical cycling mechanisms in its waters.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic Insights into Eimeria tenella-Induced Host Cell Apoptosis Through Modulation of the Mitochondrial Permeability Transition Pore. 柔嫩艾美球虫通过调节线粒体通透性过渡孔诱导宿主细胞凋亡的机制研究。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092139

Rui Bai, Shuying Zhu, Hui Wang, Chenyang Lv, Wenlong Zhao, Li Zhang, Yao Liu, Hanze Gao, Xiaoling Lv, Jianhui Li, Xiaozhen Cui

{"title":"Mechanistic Insights into Eimeria tenella-Induced Host Cell Apoptosis Through Modulation of the Mitochondrial Permeability Transition Pore.","authors":"Rui Bai, Shuying Zhu, Hui Wang, Chenyang Lv, Wenlong Zhao, Li Zhang, Yao Liu, Hanze Gao, Xiaoling Lv, Jianhui Li, Xiaozhen Cui","doi":"10.3390/microorganisms13092139","DOIUrl":"10.3390/microorganisms13092139","url":null,"abstract":"Coccidiosis due to Eimeria tenella remains a major constraint on the poultry industry. Previous studies have revealed that E. tenella infection triggers apoptosis in host cells. The mitochondrial permeability transition pore (MPTP) plays a pivotal role in the apoptosis and necrosis observed in infected host cells. However, the effect of MPTP opening on mitochondrial apoptotic factors remains unclear. To elucidate the dynamic changes in apoptotic signals during MPTP-mediated apoptosis in host cells infected with E. tenella, we established a chicken embryo caecal epithelial cell infection model. Cyclosporin A (CsA) was used to inhibit the MPTP. The infection rate was assessed by Hematoxylin and eosin (H&E) staining, whereas MPTP opening and the abundances of the mitochondrial apoptotic factors Smac, Endo G, and AIF were determined by flow cytometry and ELISA, respectively. The results revealed that both the degree of MPTP opening was markedly reduced in the E. tenella+CsA group compared to the E. tenella group (p < 0.05). Between 24 and 120 h post-infection (hpi), the cytoplasmic levels of Smac, Endo G, and AIF were significantly elevated in the E. tenella group compared with the control group (p < 0.05), while their mitochondrial levels were markedly decreased (p < 0.05). In contrast, mitochondrial expression of these factors was restored in the E. tenella+CsA group (p < 0.05), accompanied by a reduction in their cytoplasmic abundance (p < 0.05). These findings indicate that E. tenella promotes MPTP-dependent release of mitochondrial pro-apoptotic factors into the cytosol during the mid-to-late stages of infection, whereas pharmacological inhibition of the MPTP limits this redistribution.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dams Determine the Composition and Activity of Microbial Communities in Semiclosed Marine Basins of the White and Barents Seas, Russia. 水坝决定了俄罗斯白海和巴伦支海半封闭海盆中微生物群落的组成和活动。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092143

Alexander S Savvichev, Nikolay A Demidenko, Vitaly V Kadnikov, Alexey V Beletsky, Valeria V Belenkova, Igor I Rusanov, Pavel A Sigalevich, Daria A Ivanova

{"title":"Dams Determine the Composition and Activity of Microbial Communities in Semiclosed Marine Basins of the White and Barents Seas, Russia.","authors":"Alexander S Savvichev, Nikolay A Demidenko, Vitaly V Kadnikov, Alexey V Beletsky, Valeria V Belenkova, Igor I Rusanov, Pavel A Sigalevich, Daria A Ivanova","doi":"10.3390/microorganisms13092143","DOIUrl":"10.3390/microorganisms13092143","url":null,"abstract":"Microbiological and biogeochemical investigation of the bottom sediments of semiclosed basins was carried out at the Kislaya Guba tidal power station (Barents Sea) and in Kanda Bay (White Sea). Suppressed tidal water mixing is known to affect the hydrological regime of isolated basins, resulting in the development of oxygen-free sediments. The upper sediments of the studied bays were shown to contain higher concentrations of sulfide and methane, with increased rates of sulfate reduction, methanogenesis, and methane oxidation. The relative abundance of truly marine microorganisms decreased, while microorganisms common in anoxic sediments of meromictic basins developed. The indicator microorganisms with increased relative abundance were archaea of the genera Methanoregula and Methanosaeta. Bacteria of the class Chlorobia, Chloroflexi of the family Anaerolineaceae, and Rhodoferax-related bacteria were indicators of the stagnant seawater. Members of the genus Woeseia were counter-indicators, occurring only in marine water. In our opinion, under reasonably regulated water exchange via the dams, the ecosystems of the Kanda and Kislaya Guba bays may retain the characteristics of marine bays. Otherwise, the studied bays may become stratified basins with anoxic near-bottom water, harboring microbial communities similar to those inhabiting meromictic basins.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Ecological Trap: Biodegradable Mulch Film Residue Undermines Soil Fungal Network Stability. 生态陷阱：可生物降解的地膜残留物破坏了土壤真菌网络的稳定性。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092137

Maolu Wei, Yiping Wang, Feiyu Xie, Qian Sun, Huanhuan Shao, Xiaojie Cheng, Xiaoyan Wang, Xiang Tao, Xinyi He, Bin Yong, Dongyan Liu

{"title":"The Ecological Trap: Biodegradable Mulch Film Residue Undermines Soil Fungal Network Stability.","authors":"Maolu Wei, Yiping Wang, Feiyu Xie, Qian Sun, Huanhuan Shao, Xiaojie Cheng, Xiaoyan Wang, Xiang Tao, Xinyi He, Bin Yong, Dongyan Liu","doi":"10.3390/microorganisms13092137","DOIUrl":"10.3390/microorganisms13092137","url":null,"abstract":"Biodegradable mulching films are promoted as alternatives to traditional polyethylene films, but their environmental impacts remain controversial. This study investigates how biodegradable films affect microplastic pollution of soil, fungal community structure, and ecological network stability. We conducted a maize field experiment comparing conventional polyethylene (CF, PE) and biodegradable (BF, PLA + PBAT) film residues. We used scanning electron microscopy and high-throughput sequencing of fungal ITS genes. We assessed soil properties, microplastic release, fungal communities, and network stability through co-occurrence analysis. BF degraded rapidly, releasing microplastic concentrations much higher than CF. BF increased soil carbon and nitrogen and substantially enhanced maize biomass. However, it significantly reduced soil pH and decreased key functional fungi (saprotrophs and symbionts) abundance. The fungal ecological network complexity and stability declined significantly. Correlation analysis revealed positive associations between saprotrophic and symbiotic fungi abundance and network stability. In contrast, CF reduced some nutrient levels but improved fungal network complexity and stability. This study reveals that biodegradable films create an \"ecological trap.\" Short-term nutrient benefits mask systematic damage to soil microbial network stability. Our findings challenge the notion that \"biodegradable equals environmentally friendly.\" Environmental assessments of agricultural materials must extend beyond degradability to include microplastic release, functional microbial responses, and ecological network stability.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crossing the Barrier: Eikenella corrodens Bacteremia Following CNS Infection in a Patient Treated with Nivolumab-A Case Report and Literature Review. 跨越屏障：一名接受纳伏单抗治疗的患者中枢神经系统感染后，艾肯氏菌会腐蚀菌血症——病例报告和文献综述。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092135

Terenzio Cosio, Cataldo Maria Mannavola, Barbara Fiori, Matteo Zelinotti, Francesco Taccari, Brunella Posteraro, Tiziana D'Inzeo, Maurizio Sanguinetti

{"title":"Crossing the Barrier: Eikenella corrodens Bacteremia Following CNS Infection in a Patient Treated with Nivolumab-A Case Report and Literature Review.","authors":"Terenzio Cosio, Cataldo Maria Mannavola, Barbara Fiori, Matteo Zelinotti, Francesco Taccari, Brunella Posteraro, Tiziana D'Inzeo, Maurizio Sanguinetti","doi":"10.3390/microorganisms13092135","DOIUrl":"10.3390/microorganisms13092135","url":null,"abstract":"Eikenella corrodens is a facultative anaerobic Gram-negative bacillus, part of the normal oropharyngeal flora, with opportunistic pathogenic potential particularly in immunocompromised hosts. The progression from localized intracranial infections such as cerebritis and subdural empyema to secondary bloodstream infection represents a rare but clinically significant pathway, especially in immuno-compromised patients. Here, we report a case of secondary E. corrodens bacteremia following left temporal cerebritis and ipsilateral subdural empyema in a 50-year-old man with advanced nasopharyngeal carcinoma treated with nivolumab. The patient presented neurological deficits and systemic inflammatory response, suggesting for a bacterial infection. Neuroimaging confirmed the intracranial infectious foci and blood cultures identified E. corrodens via MALDI-TOF MS and 16S rRNA gene sequencing. We discuss how cancer-associated immune dysregulation and immune checkpoint inhibition could modulate host susceptibility and clinical presentation of infection, potentially facilitating microbial dissemination across compromised blood-brain barriers. Additionally, we examine the cases of E. corrodens bacteremia secondary to CNS and head and neck infections. This case underscores the importance of heightened clinical vigilance for secondary bacteremia in oncologic patients with CNS infections and highlights the need for integrated microbiological and radiological assessment to optimize outcomes.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Retrospective Study on Coinfections, Antimicrobial Resistance, and Mortality Risk Among COVID-19 Patients (2020-2021) with Consideration of Long-COVID Outcomes. 考虑COVID-19长期结局的COVID-19患者共感染、抗生素耐药性和死亡风险的回顾性研究（2020-2021）

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092141

Carlos Rescalvo-Casas, Rocío Fernández-Villegas, Marcos Hernando-Gozalo, Laura Seijas-Pereda, Lourdes Lledó García, Lars Arendt-Nielsen, Juan Cuadros-González, Ramón Pérez-Tanoira

{"title":"A Retrospective Study on Coinfections, Antimicrobial Resistance, and Mortality Risk Among COVID-19 Patients (2020-2021) with Consideration of Long-COVID Outcomes.","authors":"Carlos Rescalvo-Casas, Rocío Fernández-Villegas, Marcos Hernando-Gozalo, Laura Seijas-Pereda, Lourdes Lledó García, Lars Arendt-Nielsen, Juan Cuadros-González, Ramón Pérez-Tanoira","doi":"10.3390/microorganisms13092141","DOIUrl":"10.3390/microorganisms13092141","url":null,"abstract":"Coinfections in COVID-19 patients can worsen disease severity by enhancing SARS-CoV-2 replication and proinflammatory cytokine levels. This study analyzes the characteristics of coinfected COVID-19 patients across the pandemic and their association with in-hospital mortality. We retrospectively examined data from 351 COVID-19 patients hospitalized in a Spanish secondary hospital between March 2020 and February-March 2021. Nasopharyngeal swabs from 340 patients were analyzed using multiplex RT-PCR to identify 26 respiratory pathogens. A total of 136 patients were coinfected with 191 bacteria (100 Gram-negative and 91 Gram-positive), 20 viruses, 18 fungi, and 1 protist. In 2021, empirical cephalosporin use increased (p = 0.009). The incidence of enterococcal coinfections tripled from 2020 to 2021 (p < 0.001). In 2021, a greater proportion of patients experienced urine (p = 0.001) and bloodstream (p = 0.010) coinfections. In 2020, there was one bloodstream infection, while in 2021, there were seven, with half of them being fatal. Coinfected patients experienced longer hospital stays and higher odds of long COVID (p < 0.001; p = 0.014; p = 0.045). Non-respiratory coinfections in 2021 correlated with increased mortality (p = 0.002). Antimicrobial resistance remained stable (p = 0.149). The rise in cephalosporin use correlated with increased Enterococcus infections, notably bloodstream infections, which were linked to mortality (p = 0.016). In 2021, coinfections were linked to prolonged hospital stays and an increased risk of mortality in our patient cohort.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Genomics and Metabolomics Analyses Provide New Insights into the Molecular Basis of Plant Growth Promotion by Pantoea agglomerans. 综合基因组学和代谢组学分析为Pantoea团聚体促进植物生长的分子基础提供了新的见解。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092138

Anna Grazia Ficca, Francesca Luziatelli, Renée Abou Jaoudé, Maurizio Ruzzi

{"title":"Integrative Genomics and Metabolomics Analyses Provide New Insights into the Molecular Basis of Plant Growth Promotion by Pantoea agglomerans.","authors":"Anna Grazia Ficca, Francesca Luziatelli, Renée Abou Jaoudé, Maurizio Ruzzi","doi":"10.3390/microorganisms13092138","DOIUrl":"10.3390/microorganisms13092138","url":null,"abstract":"Plant Growth-Promoting Rhizobacteria (PGPR) are emerging as a sustainable alternative in agriculture due to their environmentally friendly properties and their ability to enhance crop productivity. Among these, Pantoea agglomerans has gained attention for its versatility as both a biofertilizer and a biocontrol agent. In this study, we use comparative genomics to gain insight into the genetic diversity and functional specialization of members of this species. The pan-genome analysis of 20 representative P. agglomerans strains revealed that 32% of the genes constitute the core genome (2856 out of 8899), while the remaining 68% are classified as accessory or singleton genes, indicating a high level of genomic diversity within the species. Functional annotation showed that core genes are predominantly involved in central metabolic processes, whereas genes associated with specialized metabolic functions are found within the accessory and singleton categories. The comparative analysis demonstrated a mosaic distribution of genes related to nitrogen and sulfur metabolism, heavy metal resistance, defense mechanisms, and oligopeptide uptake, suggesting niche-specific adaptations and metabolic capabilities within this species. Exometabolome profiling of strains associated with different hosts, specifically plant (C1) or human (DSM3493T), demonstrated that omics-centered approaches can be utilized to select P. agglomerans strains tailored to specific agronomic requirements.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Analysis of Grapevine Fanleaf Virus, Grapevine Virus A, and Grapevine Leafroll-Associated Virus 3 in Kazakhstan. 哈萨克斯坦葡萄扇叶病毒、葡萄病毒A和葡萄叶相关病毒3的系统发育分析。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092142

Ivan G Frolov, Karlygash P Aubakirova, Zhibek N Bakytzhanova, Akbota Rakhatkyzy, Laura S Yerbolova, Nurbol N Galiakparov

{"title":"Phylogenetic Analysis of Grapevine Fanleaf Virus, Grapevine Virus A, and Grapevine Leafroll-Associated Virus 3 in Kazakhstan.","authors":"Ivan G Frolov, Karlygash P Aubakirova, Zhibek N Bakytzhanova, Akbota Rakhatkyzy, Laura S Yerbolova, Nurbol N Galiakparov","doi":"10.3390/microorganisms13092142","DOIUrl":"10.3390/microorganisms13092142","url":null,"abstract":"Grapevine viruses reduce harvests and degrade fruit quality, but their genetic diversity in Kazakhstan has remained unexplored. We collected symptomatic leaves from local vineyards and recovered eleven fragments of the coat-protein gene: one from grapevine fanleaf virus, five from grapevine virus A, and five from grapevine leafroll-associated virus 3. After Sanger sequencing, we compared these fragments with more than one thousand international counterparts to place the Kazakh strains on the global family tree. The results reveal a clear spectrum of genetic diversity that mirrors each virus's route of spread. Grapevine virus A, which is moved both mechanically and by insects, proved the most variable; grapevine fanleaf virus, carried by dagger nematodes and pruning sap, had intermediate variability; and grapevine leafroll-associated virus 3, moved only by mealybugs and scales, was highly conserved. All Kazakh sequences fell inside established foreign lineages, showing that the viruses were imported multiple times rather than evolving locally. Grapevine virus A will require broad-coverage or multiplex PCR primers to avoid false negatives, whereas the stable leafroll virus can be monitored with a single high-sensitivity assay. Combined with vector management-mealybug control for leafroll, and nematode testing for fanleaf-these data lay the groundwork for a national clean-plant program and more resilient vineyards across Central Asia.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological and Microbiological Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolates in a Regional Greek Hospital: A Retrospective Study. 希腊某地区医院产碳青霉烯酶肺炎克雷伯菌分离株的流行病学和微生物学特征：回顾性研究。

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092132

Pandora Tsolakidou, Maria Chatzidimitriou

{"title":"Epidemiological and Microbiological Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolates in a Regional Greek Hospital: A Retrospective Study.","authors":"Pandora Tsolakidou, Maria Chatzidimitriou","doi":"10.3390/microorganisms13092132","DOIUrl":"10.3390/microorganisms13092132","url":null,"abstract":"Carbapenemase-producing Klebsiella pneumoniae (CRKP) is a critical public health threat, particularly in Greece, where high prevalence limits therapeutic options. This retrospective study analyzed 26 CRKP isolates recovered at the General Hospital of Volos between July 2024 and January 2025, aiming to correlate carbapenemase phenotypes with clinical and epidemiological parameters. Demographic, clinical, and microbiological data were extracted from patient records, and isolates underwent phenotypic carbapenemase detection, antimicrobial susceptibility testing, and molecular characterization using real-time PCR; four isolates were further analyzed using whole-genome sequencing. CRKP was detected across multiple hospital departments, notably in the Emergency Department (n = 5) and Intensive Care Unit (n = 6). KPC producers predominated (n = 9), followed by NDM (n = 6), VIM (n = 1), and OXA-48 (n = 6). All VIM- or NDM + VIM-positive cases were associated with mortality. High-risk clones, including ST15, ST11, and ST307, were identified, with one ST15 isolate harboring blaNDM-1, blaVIM-1, and chromosomal colistin resistance; this is the first such report in Greece. Colistin and gentamicin were the most active agents in vitro; three isolates were pan-drug-resistant. The findings highlight significant CRKP circulation outside ICUs, the role of horizontal gene transfer in resistance dissemination, and the need to expand screening and rapid diagnostics to non-ICU settings. Enhanced molecular surveillance targeted at infection control and strengthened antimicrobial stewardship programs are essential for limiting the spread of CRKP.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens-Challenged Mouse. 解淀粉芽孢杆菌SC06通过激活Wnt/β-Catenin信号通路调控肠道干细胞增殖和分化改善产气荚膜梭菌刺激小鼠肠黏膜损伤

IF 4.2 2区生物学

Microorganisms Pub Date : 2025-09-12 DOI: 10.3390/microorganisms13092136

Hongbin Deng, Si Cheng, Jiemei Fan, Haibin Hao, Dandong Fang, Weiqin Li, Qi Wang

{"title":"Bacillus amyloliquefaciens SC06 Ameliorated Intestinal Mucosal Injury by Regulated Intestinal Stem Cells Proliferation and Differentiation via Activating Wnt/β-Catenin Signal Pathway in Clostridium perfringens-Challenged Mouse.","authors":"Hongbin Deng, Si Cheng, Jiemei Fan, Haibin Hao, Dandong Fang, Weiqin Li, Qi Wang","doi":"10.3390/microorganisms13092136","DOIUrl":"10.3390/microorganisms13092136","url":null,"abstract":"The objective of our study was to verify the intervention effect of Bacillus amyloliquefaciens SC06 on NE by constructing a C. perfringens-induced intestinal damage mouse model. A total of 40 mice were randomly assigned to four treatments: CON (basal diet), CP (basal diet + C. perfringens), SC06 + CP (basal diet + SC06 + C. perfringens) and SC06 (basal diet + SC06). Our findings indicated that SC06 supplementation was effective in maintaining the integrity of the intestinal barrier, enhancing the antioxidant capacity of the intestine, reducing the generation of an inflammatory response, and suppressing enterocyte apoptosis in the presence of C. perfringens. Furthermore, SC06 supplementation enhanced the prefoliation of intestinal stem cells (ISC) and prompted their differentiation into goblet cells and Paneth cells. Moreover, our findings indicate that SC06 promotes the proliferation of C. perfringens-induced jejunum organoids and the expression of genes and proteins associated with ISC differentiation and regeneration. The mechanism by which SC06 modulates ISCs has been validated, and the results align with those obtained in vivo. In conclusion, the findings demonstrated that SC06 stimulates the proliferation and differentiation of ISCs through the activation of the Wnt/β-catenin signaling pathway, thereby accelerating epithelial regeneration and repair.","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"13 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12472280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0