Microorganisms最新文献

{"title":"Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes.","authors":"Ning Yuan, Kang Wang, Mengyue Liang, Jia Zhou, Rui Yu","doi":"10.3390/microorganisms12122593","DOIUrl":"https://doi.org/10.3390/microorganisms12122593","url":null,"abstract":"<p><p>Existing studies have demonstrated the positive effects of nano-sized iron oxide on compost maturity, yet the impact of nano-sized iron oxide on phosphorus speciation and bacterial communities during the composting process remains unclear. In this study, pig manure and straw were used as raw materials, with biochar-supported nano-sized iron oxide (BC-Fe₃O₄NPs) as an additive and calcium peroxide (CaO₂) as a co-agent, to conduct an aerobic composting experiment with pig manure. Four treatments were tested: CK (control), F1 (1% BC-Fe₃O₄NPs), F2 (5% BC-Fe₃O₄NPs), and F3 (5% BC-Fe₃O₄NPs + 5% CaO₂). Key findings include the following. (1) BC-Fe₃O₄NPs increased compost temperatures, with F3 reaching 61℃; F1 showed optimal maturity (C/N ratio: 12.90). (2) BC-Fe₃O₄NPs promoted stable phosphorus forms; Residual-P proportions were higher in F1, F2, and F3 (25.81%, 51.16%, 51.68%) than CK (19.32%). (3) Bacterial phyla <i>Firmicutes</i>, <i>Actinobacteria,</i> and <i>Proteobacteria</i> dominated. BC-Fe₃O₄NPs altered community composition, especially on day 7. <i>Firmicutes</i> dominated CK, F1, and F3; <i>Proteobacteria</i> dominated F2. At the genus level, day 7 showed <i>Corynebacterium</i> (CK), <i>Clostridum</i> (F1, F3), and <i>Caldibacillus</i> (F2) as predominant. (4) Pearson correlation analysis revealed shifted correlations between phosphorus forms and bacterial phyla after BC-Fe₃O₄NPs addition. <i>Firmicutes</i> positively correlated with NaOH-OP in F1 during the thermophilic phase, facilitating phosphate release and adsorption by BC-Fe₃O₄NPs. The significance of correlations diminished with increasing additive concentration; in F3, all phyla positively correlated with various phosphorus forms.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Drago et al. Bacteria Living in Biofilms in Fluids: Could Chemical Antibiofilm Pretreatment of Culture Represent a Paradigm Shift in Diagnostics? <i>Microorganisms</i> 2024, <i>12</i>, 259.","authors":"Lorenzo Drago, Andrea Fidanza, Alessio Giannetti, Alessio Ciuffoletti, Giandomenico Logroscino, Carlo Luca Romanò","doi":"10.3390/microorganisms12122577","DOIUrl":"https://doi.org/10.3390/microorganisms12122577","url":null,"abstract":"<p><p>In the original publication [...].</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Newcastle Disease Virus-Vectored African Swine Fever Virus Antigen Cocktail Delays the Onset of ASFV-SY18 but Is Not Protective.","authors":"Qian Li, Yiqian Jiang, Min Zheng, Xuefei Sun, Lili Hui, Yanyan Zhang, Huixian Yue, Yu Qi, Siqi Li, Junnan Ke, Qixuan Li, Boli Ma, Xiaoying Jia, Fengjie Wang, Lijuan Mi, Shoufeng Zhang, Faming Miao, Shuchao Wang, Fei Zhang, Teng Chen, Rongliang Hu","doi":"10.3390/microorganisms12122590","DOIUrl":"https://doi.org/10.3390/microorganisms12122590","url":null,"abstract":"<p><p>African Swine Fever (ASF) is a highly contagious viral disease threatening the global pig industry. Currently, only two gene-deleted live attenuated vaccines are approved, exclusively in Vietnam, and their long-term effectiveness and safety are unproven, prompting the need for safer alternatives. This study assessed a cocktail of African Swine Fever Virus (ASFV) antigens delivered via a recombinant Newcastle Disease Virus (rNDV) vector against the genotype II ASFV-SY18. Antigens pB602L, pEP84R, and p22 (pKP177R) were selected based on virus neutralization and lymphocyte proliferation assays in mice and combined with capsid protein p72 (pB646L) for vaccination and challenge in pigs. The antigen cocktail delayed ASF symptoms by 3-4 days but did not prevent the lethal ASFV-SY18 infection. Significant ASFV-specific gamma interferon (IFN-γ) positive responses and NDV antibodies were detected post-inoculation, showing an induced immune response, though ASFV-specific p72 antibodies were absent. The cocktail did not cause cytokine imbalance, indicating the vector's safety in pigs. Despite some delay in disease progression, the protection against genotype II ASFV was inadequate, underscoring the need to select more effective antigens and enhance immune responses for virus-vectored vaccines.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11679766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low 25-Hydroxyvitamin D Post-Kidney Transplant Is Associated with Increased Risk of BK Polyomavirus-Associated Nephropathy.","authors":"Suseela A Raj, Angela L Zhou, Ekaterina Fedorova, Zhongyu Yuan, Didier A Mandelbrot, Brad C Astor, Sandesh Parajuli","doi":"10.3390/microorganisms12122588","DOIUrl":"https://doi.org/10.3390/microorganisms12122588","url":null,"abstract":"<p><p>BK viremia (BKPyV-DNAemia) and nephropathy (BKPyVAN) are significant causes of morbidity and mortality in kidney transplant recipients (KTRs). Vitamin D supports immune function, yet low 25-hydroxyvitamin D [25(OH)D] is common among KTRs. The association between serum 25(OH)D, measured 61 days to 2 years post-transplant, and subsequent incident BKPyV-DNAemia and BKPyVAN was examined in KTRs without previous BKPyV-DNAemia or BKPyVAN, respectively. Out of 3308 KTRs, 399 (12%) were vitamin D deficient [25(OH)D ≤ 20 ng/mL], and 916 (27.7%) were insufficient [25(OH)D 21-29 ng/mL]. A total of 184 KTRs developed BKPyV-DNAemia and 44 developed BKPyVAN. The incidence rate (/100 person-years) for BKPyV-DNAemia was 2.88 in the 25(OH)D sufficient group, 2.22 in the insufficient group, and 2.37 in the deficient group. The incidence rate (/100 person-years) for BKPyVAN was 0.30 in the 25(OH)D sufficient group, 0.75 in the insufficient group, and 1.28 in the deficient group. Vitamin D deficiency (adjusted hazard ratio [aHR] compared to 25(OH)D sufficiency: 3.92; 95% CI: 1.66-9.23) and insufficiency (aHR: 2.22; 95% CI: 1.11-4.45) remained significantly associated with the incidence of BKPyVAN after adjustment for baseline characteristics. Low serum 25(OH)D was associated with an increased risk of BKPyVAN but not BKPyV-DNAemia.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrons in the Age of Antibiotic Resistance: Evolution, Mechanisms, and Environmental Implications: A Review.","authors":"Niyaz Ali, Izhar Ali, Ahmad Ud Din, Kashif Akhtar, Bing He, Ronghui Wen","doi":"10.3390/microorganisms12122579","DOIUrl":"https://doi.org/10.3390/microorganisms12122579","url":null,"abstract":"<p><p>Integrons, which are genetic components commonly found in bacteria, possess the remarkable capacity to capture gene cassettes, incorporate them into their structure, and thereby contribute to an increase in genomic complexity and phenotypic diversity. This adaptive mechanism allows integrons to play a significant role in acquiring, expressing, and spreading antibiotic resistance genes in the modern age. To assess the current challenges posed by integrons, it is necessary to have a thorough understanding of their characteristics. This review aims to elucidate the structure and evolutionary history of integrons, highlighting how the use of antibiotics has led to the preferential selection of integrons in various environments. Additionally, it explores their current involvement in antibiotic resistance and their dissemination across diverse settings, while considering potential transmission factors and routes. This review delves into the arrangement of gene cassettes within integrons, their ability to rearrange, the mechanisms governing their expression, and the process of excision. Furthermore, this study examines the presence of clinically relevant integrons in a wide range of environmental sources, shedding light on how anthropogenic influences contribute to their propagation into the environment.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Chemical Flocculants and Chitosan as a Pre-Concentration Step in the Harvesting Process of Three Native Microalgae Species from the Canary Islands Cultivated Outdoors at the Pilot Scale.","authors":"Laura Figueira Garcia, Zivan Gojkovic, Marianna Venuleo, Flavio Guidi, Eduardo Portillo","doi":"10.3390/microorganisms12122583","DOIUrl":"https://doi.org/10.3390/microorganisms12122583","url":null,"abstract":"<p><p>Biomass harvesting represents one of the main bottlenecks in microalgae large-scale production. Solid-liquid separation of the biomass accounts for 30% of the total production costs, which can be reduced by the use of flocculants as a pre-concentration step in the downstream process. The natural polymer chitosan and the two chemical flocculants FeCl₃ and AlCl₃ were tested on freshwater <i>Chlorella sorokiniana</i> and two marine algae, <i>Dunaliella tertiolecta</i> and <i>Tetraselmis striata</i>. A preliminary screening at the laboratory scale was performed to detect the most suitable doses of flocculants. On the basis of these results, selected doses were tested on the pilot scale, using the flocculants for a pre-concentration step and the centrifugation as a second step to confirm the effectiveness of flocculants in a realistic operational environment. The biomass recoveries (R_pilot, %) of 100 L cultures were as follows: (1) for <i>T. striata</i>, R_pilot = 94.6% for 0.08 g/L AlCl₃, 88.4% for 0.1 g/L FeCl₃, and 68.3% for 0.04 g/L chitosan; (2) for <i>D. tertiolecta</i>, R_pilot = 81.7% for 0.1 g/L AlCl₃, 87.9% for 0.2 g/L FeCl₃, and 81.6% for 0.1 g/L chitosan; and (3) for <i>C. sorokiniana</i>, R_pilot = 89.6% for 0.1 g/L AlCl₃, 98.6% for 0.2 g/L FeCl₃, and 68.3% for 0.1 g/L chitosan. Flocculation reduced the harvesting costs by 85.9 ± 4.5% using chemical flocculants. Excesses of aluminum and iron in the biomass could be solved by decreasing the pH in the biomass combined with washing. This is the first study, to the best of our knowledge, that investigates the pilot-scale flocculation of three native Canarian microalgal strains. A pilot-scale pre-concentration step before centrifugation can improve the yield and reduce costs in the microalgae harvesting process.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp.","authors":"Wujie Xu, Demin Zhang, Haochang Su, Yu Xu, Xiaojuan Hu, Guoliang Wen, Yucheng Cao","doi":"10.3390/microorganisms12122581","DOIUrl":"https://doi.org/10.3390/microorganisms12122581","url":null,"abstract":"<p><p>In this study, an eight-week culture trial of <i>Penaeus vannamei</i> juveniles was conducted in commercial intensive systems to compare the impacts of biochar and molasses addition on biofloc nitrifying bacteria and inorganic nitrogen dynamics under limited water exchange conditions. During the trial, the biofloc concentration (in terms of VSS and TSS), quantities of total bacteria (TB) and total <i>Vibrio</i> (TV), and ratio of TV/TB in the culture water were lower in the biochar group compared to the molasses group. Metagenomic sequencing analysis revealed that the bacterial community composition of bioflocs showed higher α-diversity and complexity in the biochar group compared to the molasses group. Moreover, the abundance of nitrifying bacterial genera and functional genes in bioflocs was higher in the biochar group compared to the molasses group. Inorganic nitrogen dynamics showed that NH₄⁺-N and NO₂^--N were better controlled in the biochar group compared to the molasses group, as reflected by lower peaks of NH₄⁺-N and NO₂^--N and higher NO₃^--N concentrations. Excellent production performance of shrimp was achieved, which in turn proved the reliable effect of biochar addition on the mediation of inorganic nitrogen transformation through nitrifying bacteria. These results showed that biochar addition could promote biofloc nitrifying bacteria and nitrification to more effectively control harmful nitrogen for shrimp efficient production. This study provides a practical example for the biochar application in biofloc-based systems for intensive aquaculture.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11676986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic Analysis Revealing the Impact of Water Contents on the Composition of Soil Microbial Communities and the Distribution of Major Ecological Functional Genes in Poyang Lake Wetland Soil.","authors":"Yuxin Long, Xiaomei Zhang, Xuan Peng, Huilin Yang, Haiyan Ni, Long Zou, Zhong'er Long","doi":"10.3390/microorganisms12122569","DOIUrl":"https://doi.org/10.3390/microorganisms12122569","url":null,"abstract":"<p><p>Poyang Lake is the largest freshwater lake in China, which boasts unique hydrological conditions and rich biodiversity. In this study, metagenomics technology was used to sequence the microbial genome of soil samples S1 (sedimentary), S2 (semi-submerged), and S3 (arid) with different water content from the Poyang Lake wetland; the results indicate that the three samples have different physicochemical characteristics and their microbial community structure and functional gene distribution are also different, resulting in separate ecological functions. The abundance of typical ANME archaea <i>Candidatus Menthanoperedens</i> and the high abundance of <i>mcrA</i> in S1 mutually demonstrate prominent roles in the methane anaerobic oxidation pathway during the methane cycle. In S2, the advantageous bacterial genus <i>Nitrospira</i> with ammonia oxidation function is validated by a large number of nitrification functional genes (<i>amoA, hao, nxrA</i>), manifesting in that it plays a monumental role in nitrification in the nitrogen cycle. In S3, the dominant bacterial genus <i>Nocardioides</i> confirms a multitude of antibiotic resistance genes, indicating their crucial role in resistance and their emphatic research value for microbial resistance issues. The results above have preliminarily proved the role of soil microbial communities as indicators predicting wetland ecological functions, which will help to better develop plans for restoring ecological balance and addressing climate change.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular Mycorrhizal Fungi as a Salt Bioaccumulation Mechanism for the Establishment of a Neotropical Halophytic Fern in Saline Soils.","authors":"Mónica A Lugo, María A Negritto, Esteban M Crespo, Hebe J Iriarte, Samuel Núñez, Luisa F Espinosa, Marcela C Pagano","doi":"10.3390/microorganisms12122587","DOIUrl":"https://doi.org/10.3390/microorganisms12122587","url":null,"abstract":"<p><p><i>Acrostichum aureum</i> is a halophytic pantropical invasive fern growing in mangroves and swamps. Its association with arbuscular mycorrhizal fungi (AMF) has been reported in Asia. AMF and their symbiosis (AM) commonly colonise the absorption organs of terrestrial plants worldwide. Furthermore, AMF/AM are well known for their capacity to bioaccumulate toxic elements and to alleviate biotic and abiotic stress (e.g., salinity stress) in their hosts. However, the mechanisms underlying AMF involvement in the halophytism of <i>A. aureum</i> and the structures where NaCl accumulates remain unknown. This study shows that <i>A. aureum</i> forms AM in margins of natural thermal ponds in Neotropical wetlands. All mature sporophytes were colonised by AMF, with high percentages for root length (ca. 57%), arbuscules (23), and hyphae (25) and low values for vesicles (2%). In <i>A. aureum</i>-AMF symbiosis, NaCl accumulated in AMF vesicles, and CaSO₄ precipitated in colonised roots. Therefore, AM can contribute to the halophytic nature of this fern, allowing it to thrive in saline and thermal environments by capturing NaCl from fern tissues, compartmentalising it inside its vesicles, and precipitating CaSO₄.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11677415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cryptosporidium</i> Infection During Pregnancy and Effects on Pregnancy Outcomes in Israel.","authors":"Shirley Shapiro Ben David, Olga Snitser, Miriam Parizade, Noam Orvieto, Yaacov Segal, Limor Adler","doi":"10.3390/microorganisms12122572","DOIUrl":"https://doi.org/10.3390/microorganisms12122572","url":null,"abstract":"<p><p><i>Cryptosporidium</i> is a parasite that typically causes self-limited gastroenteritis. Little is known about the course of infection and its impact during pregnancy. This retrospective cohort study conducted in Israel assessed the effects of <i>Cryptosporidium</i> infection on pregnancy and obstetrical outcomes. The study population included pregnant women with a positive PCR stool test for <i>Cryptosporidium</i> and a control group of pregnant women with negative tests, matched at a 3:1 ratio based on age, gestational week, and sector. Their medical records were retrieved for symptoms, treatments, pregnancy termination, gestational age, birth weight, Apgar score, and head circumference. Fisher's exact or chi-squared tests were used to determine significance. Between January 2020 and December 2023, 2512 pregnant women underwent PCR stool testing. Of these, 93 were positive for <i>Cryptosporidium</i>, mostly in 2022. Their median age was 31 (range: 23-42 years), and 77.4% were from medium-high socioeconomic status. The most common symptom was diarrhea (90/93, 96.7%). The infection was self-limiting in all cases, with none requiring specific treatment. No significant differences were found in miscarriage rate, delivery week, birth weight, Apgar score, or head circumference compared to the controls. This study illuminates the clinical course of <i>Cryptosporidium</i> infection in pregnant women, emphasizing a generally favorable outcome without the necessity for therapeutic intervention.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":"12 12","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11678744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}