A Comparative Study of Extended Gentamicin and Tobramycin Release and Antibacterial Efficacy from Palacos and Simplex Acrylic Cements.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2025-09-17 DOI:10.3390/microorganisms13092174

Débora Coraça-Huber, Martina Humez, Klaus-Dieter Kühn

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Abstract

Antibiotic-loaded bone cements (ALBCs) are used to prevent and treat periprosthetic joint infections (PJI). This study compares the in vitro release and antibacterial effectiveness of gentamicin from Palacos^® R+G and tobramycin from Simplex^® T. Standardized cylindrical specimens of Palacos^® R+G and Simplex^® T were incubated in phosphate-buffered saline. Antibiotic release was quantified using high-performance liquid chromatography (HPLC) over 14 and 42 days. Antibacterial efficacy was assessed using inhibition zone tests (IZT) against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli over 42 days. Palacos^® R+G exhibited a significantly higher and more sustained antibiotic release of gentamicin compared to tobramycin from Simplex^® T. The cumulative release of gentamicin from Palacos^® R+G was 1.148 µg/cm², while Simplex^® T released 198.87 µg/cm² tobramycin over 14 days. Inhibition zone tests showed that Palacos^® R+G maintained antibacterial activity for 42 days, while Simplex^® T's activity diminished after 14 days. Statistical analysis confirmed significant differences in antibacterial efficacy between the two cements. Palacos^® R+G demonstrated superior gentamicin release and sustained antibacterial activity compared to tobramycin from Simplex^® T. These findings suggest that Palacos^® R+G may offer better clinical outcomes in preventing and treating PJIs.

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Palacos和Simplex丙烯酸水泥中庆大霉素和妥布霉素缓释及抗菌效果的比较研究。

载抗生素骨水泥（ALBCs）用于预防和治疗假体周围关节感染（PJI）。本研究比较了Palacos®R+G中庆大霉素和Simplex®T中妥布霉素的体外释放和抗菌效果。Palacos®R+G和Simplex®T的标准圆柱形标本在磷酸盐缓冲盐水中培养。采用高效液相色谱法（HPLC）测定14和42 d的抗生素释放量。采用抑菌区试验（IZT）对金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌进行42天的抑菌效果评价。与Simplex®T相比，Palacos®R+G的庆大霉素释放量明显更高且更持久。在14天内，Palacos®R+G的庆大霉素累积释放量为1.148µG /cm2，而Simplex®T的庆大霉素累积释放量为198.87µG /cm2。抑制区试验表明，Palacos®R+G的抑菌活性维持了42天，而Simplex®T的抑菌活性在14天后下降。统计分析证实两种水泥的抗菌效果有显著差异。与Simplex®t的妥布霉素相比，Palacos®R+G显示出更好的庆大霉素释放和持续的抗菌活性。这些研究结果表明，Palacos®R+G可能在预防和治疗PJIs方面提供更好的临床结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.