Microbial pathogenesis最新文献

{"title":"A critical review on innovative targets for signal disruption in Enterococcus faecalis infection management","authors":"Kayeen Vadakkan ,&nbsp;Gajanan Sampatrao Ghodake ,&nbsp;Chin Wei Lai ,&nbsp;Selvaraj Vijayanand ,&nbsp;Janarthanam Hemapriya","doi":"10.1016/j.micpath.2025.107876","DOIUrl":"10.1016/j.micpath.2025.107876","url":null,"abstract":"<div><div><em>Enterococcus faecalis</em> is a Gram-positive gut-associated microbe that commonly inhabits the human intestine and poses an immense threat to individuals with a compromised immune system, particularly in healthcare settings. It is also delineated for its various virulent factors, such as strong biofilm formation and resistance to multiple medications, which in turn challenge the current treatment strategies. Quorum sensing systems, particularly the Fsr and LuxS systems, play an important role in its virulence by regulating the synthesis of enzymes like gelatinase and serine protease, which help maintain biofilm stability and host tissue encroachment.</div><div><em>E. faecalis</em> biofilms are resistant to antimicrobial medications, making the bacteria challenging to eliminate. This review explores <em>E. faecalis</em> quorum sensing systems and their role in biofilm formation, emphasizing these pathways as prospective targets for therapeutic intervention. Emerging techniques, which include the application of phages, probiotics, nanoparticles, and phytochemicals, can inhibit quorum sensing, disrupt biofilm growth and reduce <em>E. faecalis</em> pathogenicity. Targeting these processes may provide beneficial substitutes or complements to standard antibiotics, especially for antibiotic-resistant bacteria. These strategies aid in improving infection control and therapeutic effectiveness toward <em>E. faecalis</em> in healthcare settings.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107876"},"PeriodicalIF":3.3,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and complete genome characterization of Bovine gammaherpesvirus-4 (BoHV-4) as the primary viral agent for respiratory infection in cattle","authors":"Eda Baldan Toker ,&nbsp;Ozer Ates ,&nbsp;Gizem Aytoğu ,&nbsp;Mevlüt Yaşar ,&nbsp;Nilay Aybey ,&nbsp;Kadir Yeşilbağ","doi":"10.1016/j.micpath.2025.107869","DOIUrl":"10.1016/j.micpath.2025.107869","url":null,"abstract":"<div><div>The <em>Bovine gammaherpesvirus type 4</em> (BoHV-4) has been detected worldwide in healthy and disease-exhibiting cattle. Notably, BoHV-4 has recently been identified in bovine respiratory system infections. This study focuses on the isolation and complete genome characterization of Bovine gammaherpesvirus-4 (BoHV-4) as a primary viral agent responsible for respiratory infections in cattle. Cytopathogenic effects were observed in MDBK cells, confirming the presence of an enveloped DNA virus in identification tests. The viral titer of isolate BoHV-4/9746 at the 7th passage level was 6.75 log₁₀ TCID₅₀/0.1 mL. PCR tests (TK gene) identified BoHV-4 as the only viral agent present, ruling out other common respiratory (BCoV, BoHV-1, BVDV, BRSV, BPIV-3, IDV, and BEV) applied to the 5th passage supernatant. WGS analysis revealed a partial LUR sequence (86870 bp) of the BoHV-4 /9746 Bursa-TR isolate, which showed high similarity (99.8%) to the reference V.test strain. The results of phylogenetic analyses applied according to partial LUR and TK gene regions also support each other, and BoHV-4 BoHV-4/9746 isolate branches among Genotype-1 sequences, similar to other European BoHV-4 strains. Cross-neutralization antibody reactions put forth a four-fold decrease in the neutralizing capacity of the BoHV-1 hyperimmune sera against the isolate BoHV-4 /9746. In conclusion, negative results of lung sample for other viral agents affecting the respiratory tract suggest that BoHV-4 may act as a primary viral agent. This study is the first report of a partial LUR genome of a Turkish BoHV-4 isolated from the respiratory tract.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107869"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitic protozoa: Biosystematics, identification, pathogenicity, causes and remedial measures","authors":"Gourav Mishra ,&nbsp;Razia Kausar ,&nbsp;Tushar Yadav","doi":"10.1016/j.micpath.2025.107867","DOIUrl":"10.1016/j.micpath.2025.107867","url":null,"abstract":"<div><div>Protozoans are widely distributed unicellular eukaryotic microorganisms that are either free-living or inhabit other organisms as ecto- or endoparasites. Parasitic protozoans-associated diseases impose huge health complications especially in developing countries and are responsible for millions of morbidities and mortality worldwide each year. These diseases are often linked to poor hygiene, inadequate sanitation, and general neglect. For diagnosis of parasitic protozoans, microscopy, combined with various staining techniques, remains the gold standard due to its simplicity and accessibility. However, newer diagnostic methods, including molecular techniques and AI-based approaches, are gaining popularity for their higher sensitivity and ability to process multiple samples simultaneously. Current anti-protozoal drugs have limitations, accompanying toxic effects further the growing emergence of drug-resistant protozoan species, has made effective treatment ever more challenging. Advance research on target-specific, multi-dimensional vaccines and drug repurposing strategies offers hope for more effective disease control and management. Preventive measures, such as promoting health education, maintaining personal hygiene, and ensuring proper sanitation, are essential for reducing the spread of protozoan infections. This review article provides an overview of major parasitic protozoans, the factors facilitating their transmission, and recent advances in their diagnosis and control strategies.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107867"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoliquiritin suppressed LPS/CuSO4-induced pro-inflammatory response on RAW264.7 cell and zebrafish","authors":"Xin-Ni Bin ,&nbsp;Jing Cai ,&nbsp;Fei-Xiang Zhang ,&nbsp;Xiao-Huan Zhang ,&nbsp;Ming-Fang He ,&nbsp;Jia-Qi Wu","doi":"10.1016/j.micpath.2025.107874","DOIUrl":"10.1016/j.micpath.2025.107874","url":null,"abstract":"<div><h3>Aim of this study</h3><div>Isoliquiritin (ISL) as one of bioactive flavonoid from licorice was reported to have bioactivities. This study aims to evaluate the anti-inflammatory properties of ISL on RAW264.7 cells and zebrafish and reveal its possible anti-inflammatory mechanism.</div></div><div><h3>Material and methods</h3><div>The anti-inflammatory activity of ISL was measured by LPS-induced RAW264.7 cells and CuSO₄-induced zebrafish models. The accumulation of reactive oxygen species (ROS) and nitric oxide (NO) in RAW264.7 cells and zebrafish were detected by H2DCFDA fluorescent probe and nitric oxide detection kit, respectively. The nuclear translocation of NF-κB p65 in LPS-induced RAW264.7 cells was detected by immunofluorescence assay. The protein levels of IL-1β, IL-6 and TNF-α in LPS-induced RAW264.7 cells were detected by ELISA assay. The recruitment of neutrophils and macrophages was observed using fluorescence microscope and quantified by ImageJ in the zebrafish inflammatory model. The expression level of inflammation-related cytokines was analyzed by quantitative real-time PCR (qRT-PCR).</div></div><div><h3>Results</h3><div>ISL reduced the accumulation of ROS and NO in LPS-induced RAW264.7 cells and CuSO₄-induced zebrafish inflammatory models, inhibited the nuclear translocation of NF-κB p65 and reduced the release of IL-1β, IL-6 and TNF-α in LPS-induced RAW264.7 cells. Furthermore, the decrease in inflammatory cell infiltration and neutrophil migration suggests the occurrence of inflammatory reactions and the anti-inflammatory effect of ISL <em>in vivo</em>. ISL reduced the expressions of inflammation-related cytokines in LPS-induced RAW264.7 cells and zebrafish inflammatory models.</div></div><div><h3>Conclusion</h3><div>ISL exerts significant anti-inflammatory effects by reducing the production of ROS and NO, inhibiting the nuclear translocation of NF-κB p65, and inhibiting the expression of inflammatory-related factors. And it is a potential treatment option for inflammatory-related diseases.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107874"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Rhodococcus equi bacteriophage ReT1 and investigation of its bactericidal effect","authors":"Toko Ishitsuka ,&nbsp;Makoto Sugiyama ,&nbsp;Hiroaki Kubota ,&nbsp;Shinji Takai ,&nbsp;Tsutomu Kakuda ,&nbsp;Yasunori Suzuki","doi":"10.1016/j.micpath.2025.107868","DOIUrl":"10.1016/j.micpath.2025.107868","url":null,"abstract":"<div><div>In recent years, multidrug-resistant <em>Rhodococcus equi</em> has emerged globally. Phage therapy has been attracting renewed attention as an alternative to antibiotics. In this study, we aimed to isolate and maintain <em>R. equi</em> bacteriophages and characterize them as a potential agent for phage therapy to treat <em>R. equi</em> infection. Phages with lytic activity, ReT1, were induced with mitomycin C from <em>R. equi</em> Kuma83-10 strain that genomic analysis revealed to possess phage-like sequences. ReT1 showed two plaque types immediately after induction, and the cloned ones were designated ReT1v-1 and ReT1v-2. These phages could be maintained in the laboratory without titer reduction, were morphologically <em>Siphoviridae</em>-like phages, and were stable under the environmental conditions expected for use as phage therapy. ReT1v-2 was altered into a lytic phage lacking the region around the integrase gene from the ReT1v-1 genome. Both phages did not differ in their host range (narrow range), that is, formed spots only in <em>R. equi</em> JID03-27, of the 18 strains examined. However, ReT1v-2 showed the most pronounced bactericidal effect against JID03-27:pVAPA strains, which infected cultured macrophages. This result was consistent in vivo, with the ReT1v-2-treated group showing growth inhibition in mice. The collection of more phages with lysogenic activity against <em>R. equi</em> and with different characteristics will lead to more options for phage cocktail formulations in anticipation of resistance. The results of this study support the possibility of developing formulations based on these phages.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107868"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulating effect of lactoferrin on antibacterial efficacy of vB_SauM-A phage against multi-drug resistant strains of Staphylococcus aureus","authors":"Milena Grzenkowicz ,&nbsp;Natalia Kaźmierczak ,&nbsp;Lidia Piechowicz ,&nbsp;Katarzyna Kosznik-Kwaśnicka","doi":"10.1016/j.micpath.2025.107872","DOIUrl":"10.1016/j.micpath.2025.107872","url":null,"abstract":"<div><div>Bacteriophages are seen as one of the most promising alternatives to antibiotic treatment in fight against drug resistant bacteria. However, as the interest in therapeutic potential of phages grew it became clear that even phages have their limitations. Narrow spectrum of activity, preference for bacteria in specific phase of metabolic activity or development of resistance mechanisms to phages may influence the efficacy of phage treatment. Therefore, in order to avoid those effects pairing bacteriophages with other antimicrobials is being researched. In our work we have paired staphylococcal phage vB_SauM-A with lactoferrin, a protein with proven antimicrobial activity and we have studied how this combination affected phage performance. We have observed that addition of 1 mg/mL of lactoferrin resulted in increased efficacy of plating of phage lysate. We have also noticed that it has influenced phage adsorption and life cycle kinetics on two out of four tested <em>Staphylococcus aureus</em> strains. Finally, <em>in vivo</em> experiment with <em>Galleria mellonella</em> showed that phage-lactoferrin mixture had higher therapeutic effect than the phage alone. Therefore, we assume that supplementation of phage cocktails with lactoferrin may increase the efficacy of the treatment. However, the subject requires more studies using different groups of phages and pathogens.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107872"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylloplane bacterial exometabolites suppress Xanthomonas axonopodis pv. punicae: Metabolomic and genomic insights for bacterial blight management in pomegranate","authors":"Vinod Chouhan,&nbsp;Mohammed Javed,&nbsp;Shanu Kumar,&nbsp;Aditi Kundu,&nbsp;Aundy Kumar","doi":"10.1016/j.micpath.2025.107865","DOIUrl":"10.1016/j.micpath.2025.107865","url":null,"abstract":"<div><div>Bacterial blight, caused by <em>Xanthomonas axonopodis</em> pv. <em>p</em><em>unicae</em>, remains a significant constraint to pomegranate cultivation, necessitating sustainable alternatives to chemical and antibiotic-based control. In this study, 24 epiphytic bacterial strains isolated from the pomegranate phylloplane were evaluated for their biocontrol potential through integrated metabolomic, volatilomic, in vitro, and greenhouse analyses. LC-MS/MS profiling revealed the presence of bioactive metabolites such as Bacillomycin D, Fengycin, and Iturin A in <em>Bacillus</em> spp.; Macrolactin (6.3 μg/mL), Macrolactin W (4.1 μg/mL), and sucrose palmitate (2.8 μg/mL) in <em>Erwinia</em>; and Cyclo (l-Pro-l-Tyr), Tryptophol, Dapdiamide A and B, and Pantocin A and B in <em>Pantoea</em>. GC-MS analysis identified (E)-3-Butylidene-4,5-dihydroisobenzofuran-1(3H)-one as a dominant volatile compound across multiple isolates. Among the strains, <em>Pantoea dispersa</em> Pg-Slp6 emerged as the most promising, exhibiting the highest phosphate solubilization (84.5 μg/mL), indole-3-acetic acid production (28.6 μg/mL), siderophore activity (CAS index 0.86), and significant pathogen suppression (72.3 % inhibition in vitro; 61.5 % disease reduction in greenhouse trials). Metabolite-mediated bioassays confirmed complete inhibition of <em>X. axonopodis</em> pv. <em>p</em><em>unicae</em> by secondary metabolites from <em>Bacillus amyloliquefaciens</em> P2-1 and <em>P. dispersa</em> Pg-Slp6. Whole-genome sequencing of <em>P. dispersa</em> Pg-Slp6 revealed gene clusters associated with secondary metabolite biosynthesis, xenobiotic degradation, and plant growth-promoting traits, affirming its multifunctional capabilities. Phylogenetic analysis based on 100 conserved protein-coding genes placed <em>P. dispersa</em> Pg-Slp6 in a distinct clade, underscoring its unique genomic identity. These findings establish <em>P. dispersa</em> Pg-Slp6 as a robust candidate for developing phylloplane-adapted microbial formulations aimed at the eco-friendly and effective management of bacterial blight in pomegranate.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107865"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bactericidal effect and mechanism of high-voltage electrostatic field (HVEF) against meat-borne spoiler Pseudomonas fragi: Cell membrane damage and oxidative stress","authors":"Bingjie Zhang ,&nbsp;Junlan Gan ,&nbsp;Yu Tao ,&nbsp;Xia Fan ,&nbsp;Keping Ye","doi":"10.1016/j.micpath.2025.107877","DOIUrl":"10.1016/j.micpath.2025.107877","url":null,"abstract":"<div><div>This study aimed to investigate the antibacterial effect of high-voltage electrostatic field (HVEF) treatment (35 kV) at different times (0, 3, 6, 9, 12, 15 min) against <em>Pseudomonas fragi</em> (<em>P. fragi</em>), and the bactericidal mechanism of HVEF was evaluated through the damage of cell wall/membrane and oxidative stress. The results indicated that with the prolongation of treatment time, the mortality rate of <em>P. fragi</em> increased and was up to 99.91 % at 15 min, and the sublethal count firstly increased and then decreased. HVEF treatment made the accumulation of intracellular reactive oxygen species, which led to bacterial cell wall damage and cell membrane perforation, increased membrane permeability and decreased fluidity, thereby causing the leakage of intracellular components. Energy limitation also appeared under HVEF stress as seen from inactivation of ATPase on the membrane. In addition, expression levels of oxidative stress-related genes (<em>sodA</em>, <em>AhpC</em>, and <em>KatE</em>) of <em>P. fragi</em> induced by HVEF were significantly upregulated while significantly upregulated in expression levels of <em>FabF</em>. The decrease in Superoxide Dismutase (SOD) activity and Glutathione/Oxidized Glutathione (GSH/GSSG) ratio further demonstrated that the lipid peroxidation of cell membrane and bacterial oxidative stress contributed to the antibacterial mechanism induced by HVEF.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107877"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helicobacter pylori associated MicroRNA and transcriptional networks in gastric pathogenesis: Exploring the epigenetic dialogue landscape","authors":"Vinay Alva ,&nbsp;Pratheek Jain ,&nbsp;Kounaina Khan ,&nbsp;Farhan Zameer ,&nbsp;Pramod Kumar P ,&nbsp;Harish Prashanth KV ,&nbsp;Shubha Gopal ,&nbsp;Vidya Niranjan ,&nbsp;Bindia Sahu ,&nbsp;Ravish H ,&nbsp;Raghu AV ,&nbsp;Raghavendra HL ,&nbsp;Dilip Apturkar K","doi":"10.1016/j.micpath.2025.107864","DOIUrl":"10.1016/j.micpath.2025.107864","url":null,"abstract":"<div><div>The pathogenicity of <em>Helicobacter pylori</em> infection is a well-documented risk factor for gastric ailments such as ulcer and cancer. However, the molecular mechanisms that underlie its pathogenic effects are only partially understood. To address the research gap, this study utilizes a comprehensive bioinformatic approach to identify key microRNAs (miRNAs) that target high-risk genes associated with <em>H. pylori</em> at an epigenetic level. The miRNAs in question, including hsa-mir-539, hsa-mir-361, hsa-mir-203a, hsa-mir-7-1, hsa-let-7f-1, hsa-mir-133a-1, and hsa-mir-186, were validated through rigorous database searches and cross-referencing. By curating the miRNet and other miRNA platforms, the study uncovered intricate regulatory networks between these miRNAs and transcription factors (TFs) such as androgen receptor (AR), BCL6, and GATA1. These interactions play a crucial role in inflammatory and carcinogenic pathways. The functional annotation of the miRNAs highlights their significant roles in essential biological processes and pathways that are relevant to <em>H. pylori</em> infection and gastric cancer progression. Overall, this integrated analysis provides critical insights into the molecular landscape of <em>H. pylori</em>-induced ulceration and carcinogenesis. This information may offer potential biomarkers and therapeutic targets for future research and clinical applications in managing gut health.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107864"},"PeriodicalIF":3.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance and virulence factors of Klebsiella quasipneumoniae, the novel sequence types (ST) 7979 and 7980 from Indonesia","authors":"Putu Yuliandari ,&nbsp;Ajeng Kusumaningtyas Pramono ,&nbsp;Novaria Sari Dewi Panjaitan ,&nbsp;Agriani Dini Pasiana ,&nbsp;Dwi Tyastuti ,&nbsp;Abdul Hadi Furqoni ,&nbsp;Sarwo Handayani ,&nbsp;Khariri Khariri ,&nbsp;Fitriana Fitriana ,&nbsp;Rita Marleta Dewi ,&nbsp;Sunarno Sunarno ,&nbsp;Nastiti Intan Permata Sari","doi":"10.1016/j.micpath.2025.107857","DOIUrl":"10.1016/j.micpath.2025.107857","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> is a human pathogen of global concern. The more recently described pathogen, <em>K. quasipneumoniae,</em> shares similar morphological characteristics with <em>K. pneumoniae</em> and is commonly misidentified as this species using conventional laboratory techniques. This study investigates the molecular characteristics of four phenotype-identified <em>K. pneumoniae</em> isolates obtained from hospital wastewater in Jakarta, Indonesia. Whole-genome sequencing (WGS) and the Average Nucleotide Identity (ANI) showed that these isolates were eventually identified as <em>K. quasipneumoniae</em> subsp. <em>quasipneumoniae</em>, a closely related species of <em>K. pneumoniae</em>. These isolates of novel ST7979 and ST7980 strains are classified as multi-drug resistant (MDR) bacteria and harbor many antibiotic-resistance genes. Interestingly, the novel ST7980 strain is carbapenem non-susceptible and harbors the <em>sul1</em> gene and the heat-stable enterotoxin gene, <em>astA</em>. The ST7979 strains have KL55 capsular type and O3b type, whereas the ST7980 strains have KL107 and O12 types. Our finding highlights the significance of identifying the <em>K. quasipneumoniae</em> strain utilizing a genomic platform. Additionally, routine surveillance is needed to monitor the hospital wastewater and avoid the spread of multidrug-resistant bacteria.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"207 ","pages":"Article 107857"},"PeriodicalIF":3.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}