Microbial pathogenesis最新文献

{"title":"Skin microbiome of Asian elephants with skin diseases during seasonal transitions.","authors":"Junmin Chen, Zixian Chen, Bo Xu, Zunxi Huang, Chengbo Zhang","doi":"10.1016/j.micpath.2025.107832","DOIUrl":"10.1016/j.micpath.2025.107832","url":null,"abstract":"<p><strong>Introduction: </strong>Wild Asian elephants (Elephas maximus), which are an endangered species, often suffer from skin diseases during seasonal transitions, which seriously affect their health. Understanding the pathogenesis of such skin diseases is critical for their prevention and treatment. It is known that skin microorganisms are closely related to host skin health.</p><p><strong>Objective: </strong>To compare the microbiotas and microbiomes of diseased and healthy skin of Asian elephants.</p><p><strong>Methods: </strong>DNA was extracted from skin swab samples from diseased and healthy Asian elephants for metagenomic sequencing. Various bioinformatic tools were used to process the raw sequencing data and identify gene sequences for functional annotation and species identification as well as to determine species abundance. Antibiotic resistance genes and virulence factors were also identified using DIAMOND.</p><p><strong>Results: </strong>Staphylococcus was highly enriched in the microbiota of diseased skin, whereas Leuconostoc predominated in that of healthy skin. Moreover, substantial differences existed between the two elephant skin groups in terms of metabolic pathways related to ATP-binding cassette transporters and TCSs and the abundance of antibiotic resistance genes and Staphylococcus-associated toxins. The substantial difference in Staphylococcus-related virulence factors was likely due to the significant enrichment of Staphylococcus in the diseased skin samples, suggesting that this bacterial genus is the causative agent of skin diseases in Asian elephants. Additionally, Leuconostoc mesenteroides, which was enriched in the healthy skin samples, has anti-inflammatory, antimicrobial, and other beneficial effects that have promising applications in the prevention, diagnosis, and treatment of skin diseases.</p><p><strong>Conclusion: </strong>This study reveals the cause of skin diseases in Asian elephants and provides a theoretical basis for improving the skin health of wild animals and expanding wildlife conservation methods and technologies.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107832"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ferrous iron transporter FeoB mediates motility, biofilm formation, and virulence in Aeromonas veronii","authors":"Yong-Chao Guan ,&nbsp;Shuang Liang ,&nbsp;Ying-Da Wang ,&nbsp;Shao-Yuan Bai ,&nbsp;Che-Bing Huang ,&nbsp;Jing-Zhi Gong ,&nbsp;Wen-Qian Shi ,&nbsp;Yuan-Huan Kang ,&nbsp;Xiao-Feng Shan ,&nbsp;Si-Yang Huang","doi":"10.1016/j.micpath.2025.108014","DOIUrl":"10.1016/j.micpath.2025.108014","url":null,"abstract":"<div><div><em>Aeromonas veronii</em> (<em>A. veronii</em>) is a widespread pathogen that can affect human, animals, and aquatic. The ferrous iron (Fe²⁺) transport system (Feo system) is essential for bacterial survival and virulence. Within this system, FeoB, a transmembrane NTPase, plays a key role in maintaining iron homeostasis. However, the role of <em>feoB</em> in the <em>A. veronii</em> is still not clear. In this study, a stable A. veronii feoB genetic mutant strain (Δ<em>feoB</em>) was constructed, and a strain complemented this mutation (C-<em>feoB</em>). Compared to the wild-type <em>A. veronii</em> strain (TH0426), Δ<em>feoB</em> exhibited reduced gentamicin sensitivity and impaired growth under iron-limited conditions. Additionally, the Δ<em>feoB</em> mutant exhibited significant reduction in motility, adhesion, and invasion capabilities. In term of virulence, the wild-type strain exhibited a 2.62-fold higher cytotoxicity toward EPC cells than Δ<em>feoB</em>. And the LD₅₀ of Δ<em>feoB</em> was 178-fold lower than that of the wild-type strain, indicating a substantial attenuation of virulence. Consistently, <em>Carassius</em> infected with Δ<em>feoB</em> displayed significantly lower bacterial loads in tissues. Moreover, the Δ<em>feoB</em> exhibited diminished antioxidant capacity. In conclusion, this study initially revealed the role of <em>feoB</em> gene in <em>A. veronii</em> TH0426, and provided a new insight into reducing bacterial infectivity and virulence by targeting the regulation of Feo systems.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"208 ","pages":"Article 108014"},"PeriodicalIF":3.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico and experimental analysis of ribosomal proteins expressions in SARS-cov-2 patients.","authors":"Farnaz Vafanezhad, Shiva Ansari Astaneh, Zahra Rashvand, Nematollah Gheibi, Seyyed Reza Mousavi, Ali Abdi, Azam Janati Esfahani, Hossein Ahmadpour-Yazdi, Hajie Lotfi","doi":"10.1016/j.micpath.2025.107814","DOIUrl":"10.1016/j.micpath.2025.107814","url":null,"abstract":"<p><p>The expression levels of ribosomal proteins (RPs), selected based on microarray data, were analyzed in severe and mild infected patients with SARS-CoV-2 virus. qRT-PCR (Quantitative reverse transcription polymerase chain reaction) was performed to measure gene expression levels. Significant down-regulation was observed in RPL27a (-0.75 ± 2.95 in severe vs. -1.71 ± 1.91 in mild), RPL26 (0.021 ± 2.12 in severe vs. -1.23 ± 2.06 in mild), and RPL14 (-1.51 ± 1.9 in severe vs. -0.79 ± 1.5 in mild) (P < 0.05). On the other hand, no significant differences were found in the expression of RPL23 (-1.6 ± 3.4 in severe vs. -2.0 ± 2.69 in mild) and RPL31 (-2.5 ± 1.77 in severe vs. -2.09 ± 2.41 in mild). A significant correlation was identified between RPL26, RPL14, and RPL27a in mild patients, while RPL23 correlated with RPL31 and RPL27a in severe patients. These findings confirm the involvement of ribosomal proteins in SARS-CoV-2 infections in both severe and mild cases, suggesting their influence on the severity of the disease.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107814"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The beneficial role of probiotics and gut microbiota in signaling pathways, immunity, apoptosis, autophagy, and intestinal barrier for effective wound healing post-burn injury.","authors":"Roya Hajialibabaei, Fatemeh Ghaffarian Sayeli, Esmat Aghadavood, Mohsen Poudineh, Azad Khaledi, Khadijeh Bamneshin","doi":"10.1016/j.micpath.2025.107816","DOIUrl":"10.1016/j.micpath.2025.107816","url":null,"abstract":"<p><p>Severe burn injuries are associated with complex systemic disturbances, including profound immune dysregulation, compromised intestinal barrier function, and alterations in gut microbiota composition-factors that collectively contribute to impair wound healing and increased mortality. While broad-spectrum antibiotics are routinely employed to combat infection, their use may inadvertently aggravate mucosal barrier dysfunction and facilitate bacterial translocation. Emerging evidence underscores the potential of probiotics in restoring intestinal homeostasis and enhancing immune responses in critically ill populations; however, their application within the context of burn care remains insufficiently studied. This review seeks to address this knowledge gap by evaluating the therapeutic utility of probiotic supplementation in supporting gastrointestinal barrier integrity, attenuating inflammatory responses, and facilitating post-burn recovery. A comprehensive narrative review of relevant literature was performed via PubMed and Google Scholar, targeting studies involving microbiota, probiotics, and burn trauma. Probiotic strains, particularly Lactobacillus and Bifidobacterium, have been shown to modulate cytokine profiles, boost secretory IgA production, enhance epithelial regeneration, and influence key signaling pathways such as PI3K/Akt/mTOR, which are integral to regulating apoptosis and autophagy. Experimental models indicate that probiotics can decrease bacterial translocation and systemic inflammation, reinforce tight junction architecture, and elevate short-chain fatty acid concentrations. Notably, probiotic administration in burn models has resulted in up to a 75 % reduction in infection-related mortality and enhanced anti-inflammatory responses via IL-10 and Th1 pathway activation. Moreover, adjunctive use of probiotics in topical formulations has shown efficacy in promoting wound healing in both diabetic and surgical settings. Collectively, these findings highlight the promise of probiotics as a complementary therapeutic approach in burn management, offering multifaceted benefits in reducing infection, supporting tissue regeneration, and improving clinical outcomes.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107816"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPV16 E6 and Chlamydia trachomatis Pgp3 proteins inhibit TNF-α-induced apoptosis through Daxx-mediated MDM2-p53 pathway.","authors":"Yaqi Liao, Ranzhong Chen, Erqun Tang, Shuangyang Tang","doi":"10.1016/j.micpath.2025.107849","DOIUrl":"10.1016/j.micpath.2025.107849","url":null,"abstract":"<p><strong>Background and aims: </strong>As a common pathogen of sexually transmitted diseases, Chlamydia trachomatis (C. trachomatis) may be a cofactor in the progression of cervical cancer induced by persistent HPV infection, but its molecular mechanism is unclear. The aim of this study is to explore whether HPV16 E6 and C. trachomatis Pgp3 proteins inhibit apoptosis through the Daxx-mediated MDM2-p53 pathway, in order to reveal the potential mechanism of their synergistic effects.</p><p><strong>Methods: </strong>The HeLa cell model was used to analyze the effects of HPV16 E6 and Pgp3 proteins on Daxx expression and the MDM2-p53 pathway, combined with interfering with Daxx, TNF-α-induced apoptosis assay and Nutlin-3a treatment to validate the function of key molecules.</p><p><strong>Results: </strong>HPV16 E6 and C. trachomatis Pgp3 proteins can upregulate the expression of Daxx proteins individually or synergistically, and the inhibitory on TNF-α-induced apoptosis may be superimposed. Interference with Daxx partially reversed the apoptosis inhibition of HPV16 E6 and Pgp3, suggesting that it may act by promoting MDM2 phosphorylation and p53 degradation. Moreover, Nutlin-3a treatment can attenuate the apoptosis inhibitory of HPV16 E6 and Pgp3, further supporting the involvement of MDM2-p53 pathway.</p><p><strong>Conclusions: </strong>This study suggests that HPV16 E6 and C. trachomatis Pgp3 proteins can inhibit TNF-α-induced apoptosis through Daxx-mediated MDM2-p53 pathway, which may provide a mechanistic foundation for the study of HPV16 and C. trachomatis co-infection on cervical cancer progression.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107849"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the mechanisms of drug resistance of Mycobacteriumtuberculosis.","authors":"Muhammad Sikandar, Li Xing","doi":"10.1016/j.micpath.2025.107861","DOIUrl":"10.1016/j.micpath.2025.107861","url":null,"abstract":"<p><p>Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), has developed different mechanism of action against antimicrobial drug therapies, posing a significant threat to effective treatment against Mtb. This review article provides an overview of the recent advancements in the mechanism of drug resistance in Mtb and highlights how drugs target specific cellular processes. Additionally, we also discuss the intrinsic resistance mechanisms employed by Mtb and how alterations in drug target sites contribute to treatment failure.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107861"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based assessment of antimicrobial resistance, virulence-associated genes, and prophage diversity across clinical Salmonella Typhimurium isolates in the south of Tunisia.","authors":"Sonia Ktari, Fahmi Smaoui, Boutheina Ksibi, Sonda Maalej, Faouzia Rhimi, Simon Le Hello, Adnene Hammami","doi":"10.1016/j.micpath.2025.107850","DOIUrl":"10.1016/j.micpath.2025.107850","url":null,"abstract":"<p><p>Salmonella Typhimurium is a significant foodborne pathogen and a leading cause of gastroenteritis in humans and animals. Among its various lineages, the DT104 strain is particularly notable for its high level of antimicrobial resistance, making it a significant global concern. Here, we used whole-genome sequencing to investigate the antimicrobial resistance and virulence-associated genomic features of clinical S. Typhimurium DT104, and we compared them to non-DT104 strains recovered from southern Tunisia between 2000 and 2013. Among 88 S. Typhimurium isolates, 39.8 % were definitive type DT104. Antimicrobial resistome analysis revealed clinically significant genes including bla_CARB-2, bla_TEM-1B, aadA2, sul1, sul2, tet(G), floR, dfrA5, and biocide resistance gene qacEΔ1. β-lactams resistance in DT104 and non-DT104 strains was associated with bla_CARB-2 (91 %) and bla_TEM-1 (34 %) genes, respectively. Resistance mutations in GyrA (D87N or S83R) were identified in three isolates with reduced susceptibility to nalidixic acid. Among 10 intact prophages, the main prophages detected were Gifsy_1 (96.6 %), and Gifsy_2 (95.5 %). Virulence gene screening suggested that DT104 is not more pathogenic than non-DT104 isolates. Furthermore, virulence plasmid genes were identified in approximately 95 % of isolates. Ten Salmonella pathogenicity islands (SPIs) encoding virulence factors were detected in all isolates except SPI-14 and CS54_island. SNP analysis revealed clade-specific missense mutations within plasmid virulence, SPI-1, SPI-2, and fimbriae genes. Genomic characterization sheds light on the diversity of genetic elements contributing to clinical S. Typhimurium strains' pathogenicity and antibiotic resistance from Tunisia. Therefore, continuous genomic surveillance is an important tool for preserving human health.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107850"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl-2,4-dihydroxybenzoate induces transcriptomic reprogramming in Fusarium oxysporum f. sp. cubense and defense gene responses in Kadali banana","authors":"B.R. Ajesh ,&nbsp;S. Nakkeeran ,&nbsp;P. Renukadevi ,&nbsp;R.U. Krishna Nayana ,&nbsp;Suhail Ashraf ,&nbsp;R. Udhayakumar ,&nbsp;Khalid E. Hamed","doi":"10.1016/j.micpath.2025.108013","DOIUrl":"10.1016/j.micpath.2025.108013","url":null,"abstract":"<div><div>The emergence of fungicide resistance and environmental concerns with conventional chemicals necessitate the identification of novel antifungal compounds. Methyl-2,4-dihydroxybenzoate (MDHB), a hydroxybenzoate derivative, exhibits potent antifungal activity against <em>Fusarium oxysporum</em> f. sp. <em>cubense,</em> the causative agent of Panama disease in bananas. To uncover its molecular mechanism of action, we performed comprehensive transcriptomic profiling of <em>Foc</em> treated with 100 ppm MDHB using RNA sequencing technology. The analysis revealed 173 differentially expressed genes (DEGs), comprising 121 upregulated and 52 downregulated genes (|log₂FC| &gt; 1, FDR &lt;0.05). Gene Ontology analysis showed that MDHB exerts multifaceted antifungal activity through membrane destabilization (47 membrane-associated genes affected), suppression of energy metabolism via downregulation of ATP-binding proteins and proton-pumping ATPases, oxidative stress induction through impairment of superoxide dismutase (<em>SOD2</em>) and antioxidant systems. It also disrupted protein synthesis machinery including ribosomal proteins and elongation factors. MDHB further compromised cellular transport by downregulating ABC transporters (<em>STE6</em>) and major facilitator superfamily transporters (<em>PTR2</em>), and disrupted N-glycosylation <em>via</em> repression of <em>SRD5A3</em> and <em>RFT1</em> genes. Plant defense gene expression analysis revealed enhanced immune responses in banana, with significant upregulation of <em>WRKY</em> transcription factors (5.9-fold), <em>LECTIN</em> receptors (5.3-fold), and <em>MAPK</em> signaling components (5.5-fold). Glasshouse trials confirmed MDHB's efficacy, reducing disease severity from 100 % to 4.4 % and limiting disease incidence to 16 % compared to 100 % in pathogen-inoculated controls. This study provides the first transcriptomic insights into <em>Foc</em>'s response to MDHB, revealing a multi-target mechanism disrupting key cellular functions while enhancing plant defense, establishing MDHB as a promising antifungal candidate for sustainable <em>Fusarium</em> wilt management.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"208 ","pages":"Article 108013"},"PeriodicalIF":3.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of antifungal formulations using silver nanoparticles and natural antimicrobials for food preservation","authors":"Seyedeh Elmira Moosavi ,&nbsp;Annie Castonguay ,&nbsp;Steven Laplante ,&nbsp;Elizabeth Ramirez Rodriguez ,&nbsp;Stephane Salmieri ,&nbsp;Monique Lacroix","doi":"10.1016/j.micpath.2025.108011","DOIUrl":"10.1016/j.micpath.2025.108011","url":null,"abstract":"<div><div>This study aimed to evaluate natural antifungal agents for controlling food spoilage caused by <em>Botrytis cinerea</em>, <em>Rhizopus stolonifer</em>, and <em>Alternaria brassicae</em>. Various plant-derived essential oils (EOs), including Mediterranean, Pan Tropical, Greek, Canada pine, Nepal pine, clove bud, cumin, Moroccan, and others, as well as 2 citrus <strong>extracts</strong>, and spherical silver <strong>nanoparticles (AgNPs: AGPPH and AGC 0.5) were</strong> tested for their antifungal properties. A broth microdilution assay was employed to determine the minimum inhibitory concentrations (MICs) of individual EOs and AgNPs. The potential interactions between EOs and AgNPs were evaluated using the checkerboard method by calculating fractional inhibitory concentration (FIC) indices. The chemical compositions of selected EOs (Mediterranean, Greece, Pan Tropical, and Morocco) were analyzed using high-performance liquid chromatography (HPLC), revealing that over 97 % of their content comprised 15 major components, including trans-cinnamaldehyde, trans-p-methoxy cinnamaldehyde, trans-o-methoxy cinnamaldehyde, cis-cinnamaldehyde, cinnamyl acetate, coumarin, thymol, carvacrol, p-cymene, γ-terpinene, linalool, <em>β</em>-caryophyllene, α-pinene, myrcene and α-terpinene. Based on MIC and FIC results, three active formulations (AFs) were developed: AF1 (Mediterranean EO/Greece EO/Pan Tropical EO/AGC 0.5, 0.3:0.3:2.5:0.05), AF2 (Morocco EO/Greece EO/Pan Tropical EO/AGPPH, 0.3:0.3:2.5:0.05), and AF3 (Morocco EO/Mediterranean EO/Pan Tropical EO/AGC 0.5, 0.3:0.3:2.5:0.05). Their antifungal efficacy was assessed through MIC and agar diffusion assays. The formulations demonstrated strong antifungal activity, inhibiting <em>R. stolonifer</em> (80.7–89.0 %), <em>B. cinerea</em> (96.3–100 %), and <em>A. brassicae</em> (94.3–100 %). These findings suggest that these EO/AgNP-based formulations could serve as effective green antifungal agents for food preservation.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"208 ","pages":"Article 108011"},"PeriodicalIF":3.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus","authors":"Teresa Cardona-Cabrera ,&nbsp;Carmen González-Azcona ,&nbsp;Paula Eguizábal ,&nbsp;Olga Alexandrou ,&nbsp;Giorgos Catsadorakis ,&nbsp;Panagiotis Azmanis ,&nbsp;Carmen Lozano ,&nbsp;Ursula Höfle ,&nbsp;Carmen Torres","doi":"10.1016/j.micpath.2025.108009","DOIUrl":"10.1016/j.micpath.2025.108009","url":null,"abstract":"<div><div>In 2022, an avian influenza outbreak caused a massive mortality of Dalmatian pelicans (<em>Pelecanus crispus</em>, hereafter DP) in their world's largest breeding colony located at Mikri Prespa Lake (Greece), while great white pelicans (<em>Pelecanus onocrotalus,</em> hereafter GWP) were not affected. The aim of this study was to describe bacterial diversity and antimicrobial resistance carriage from choanal clefts of DPs and GWPs, to elucidate differences between the two species of pelicans and age groups. Fifty-two choanal swab samples were collected from 31 nestlings (N) (20 DP/11 GWP) late in the 2022 breeding season, and 21 adults (A) DPs (DP-A) in early 2023. Samples were seeded in general and chromogenic selective media. Isolates were identified by MALDI-TOF mass spectrometry and antimicrobial susceptibility was evaluated by disk diffusion method for staphylococci/mammaliicocci, enterococci and Enterobacterales. A total of 248 non-repetitive isolates (46A/202N) were recovered, representing 39 bacterial species from 18 genera. A very high prevalence of <em>S. aureus</em> (80.8 % of samples tested) was detected in both age groups (85.7 %A/77.4 %N), with nestlings also showing greater staphylococcal/mammaliicoccal diversity. Most isolates recovered were susceptible to all the antibiotics tested, and none <em>S. aureus</em> was methicillin-resistant. Nevertheless, one multi-drug resistant (MDR) CTX-M-15-producing <em>E. coli</em>-ST69 isolate was found in one DP-A. This study revealed a high rate of antimicrobial susceptibility and staphylococcal/mammaliicoccal diversity in choanal samples of pelicans, with the occasional detection of MDR isolates of concern, like the extended-spectrum beta-lactamase-producing <em>E. coli</em> strain.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"208 ","pages":"Article 108009"},"PeriodicalIF":3.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}