Effects of probiotics on gut microbiota in diarrheal Chinese pangolins (Manis pentadactyla) analyzed through 16S rRNA sequencing

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-18 DOI:10.1016/j.micpath.2025.108036

Lina Zhang , Qihui Li , Yan Hua

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引用次数: 0

Abstract

The gut microbiota plays important roles in intestinal health, but the changes and functions of microbiota in diarrheal pangolins remain poorly understood. This study analyzed gut microbiota changes in 12 Chinese pangolins (Manis pentadactyla) from diarrhea to recovery using 16S rRNA amplicon sequencing. Fecal samples of Chinese pangolins were achieved at day 1 (diarrhea) and day 5 (post-probiotic treatment). Results showed significantly increased microbial diversity and structural shifts in gut microbiota following probiotics treatment. A total of 18 phyla were identified in the fecal microorganisms of Chinese pangolins. Firmicutes, Proteobacteria, Bacteroidetes, and Fusobacteria were predominant bacteria. At the genus level, Clostridium_sensu_stricto_1, Bacteroides, Clostridium, Streptococcus, Escherichia-Shigella, Megasphaera, Cellulosilyticum, and Lactococcus were the predominant in both diarrheal groups and recovery group. After probiotic treatment, Streptococcus (P = 0.00001), and Escherichia-Shigella (P = 0.04) were significantly decreased, and similar phenomenon was observed in Limosilactobacillus (P = 0.0003) and Lactobacillus (P = 0.0002). In conclusion, this study provides a comprehensive analysis of gut microbiota shifts during diarrhea treatment in Chinese pangolins, indicating that probiotic agents could be an adjuvant therapy when treating diarrhea in captive pangolins.

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利用16S rRNA测序分析益生菌对腹泻型穿山甲肠道菌群的影响

肠道菌群在肠道健康中发挥着重要作用，但对腹泻穿山甲肠道菌群的变化和功能仍知之甚少。本研究采用16S rRNA扩增子测序技术，分析了12只穿山甲从腹泻到康复期间肠道菌群的变化。在第1天（腹泻）和第5天（益生菌治疗后）采集穿山甲粪便样本。结果显示，益生菌治疗后，肠道菌群的微生物多样性显著增加，结构发生变化。在中国穿山甲粪便微生物中共鉴定出18门。优势菌群为厚壁菌门、变形菌门、拟杆菌门和梭杆菌门。在属水平上，腹泻组和恢复组均以梭菌属（clostridium_sensen_stricto_1）、拟杆菌属（Bacteroides）、梭菌属（Clostridium）、链球菌（Streptococcus）、志贺氏杆菌属（Escherichia-Shigella）、Megasphaera、Cellulosilyticum)和乳球菌（Lactococcus）为主。益生菌处理后，链球菌（P=0.00001）和志贺氏杆菌（P=0.04）显著降低，Limosilactobacillus （P=0.0003）和乳酸菌bacillus （P=0.0002）也有类似现象。综上所述，本研究全面分析了中国穿山甲腹泻治疗过程中肠道菌群的变化，提示益生菌制剂可作为治疗圈养穿山甲腹泻的辅助疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)