Microbial pathogenesis最新文献

{"title":"Corrigendum to \"Investigation of interspecies crosstalk between probiotic Bacillus subtilis BR4 and Pseudomonas aeruginosa using metabolomics analysis\" [Microb. Pathog. 166 (2022) 105542].","authors":"Seenivasan Boopathi, Rajesh Vashisth, Ashok Kumar Mohanty, Ai-Qun Jia, Natesan Sivakumar, Naiyf S Alharbi, Jamal M Khaled, Annie Juliet, Jesu Arockiaraj","doi":"10.1016/j.micpath.2025.107844","DOIUrl":"10.1016/j.micpath.2025.107844","url":null,"abstract":"","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107844"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocytosis in Fusarium graminearum requires coordination of the motor domain and two tail homology domains of myosin-1.","authors":"Qin-Rong Tan, Lin-Lin Yao, Min Yuan, Shaopeng Sun, Xiang-Dong Li","doi":"10.1016/j.micpath.2025.107841","DOIUrl":"10.1016/j.micpath.2025.107841","url":null,"abstract":"<p><p>Fusarium graminearum is a major pathogen for the outbreak of Fusarium head blight disease. Recently, it was found that phenamacril (a Fusarium-specific fungicide) specifically inhibits the motor function of F. graminearum myosin-1 (FgMyo1). By using the FgMyo1-specific inhibitor phenamacril and genetic manipulation of FgMyo1 gene, we investigated the roles of each FgMyo1 domains (motor domain, TH1 domain, TH2 domain, SH3 domain, and CA domains) in supporting F. graminearum growth, with a special focus on endocytosis and subapical localization of FgMyo1. We demonstrate that FgMyo1^TH2 (a truncated FgMyo1 containing the motor domain, IQ motifs, TH1 and TH2 domains) is sufficient to support endocytosis of F. graminearum and subapical localization of FgMyo1. Biochemical analysis and electron microscopy revealed that FgMyo1^TH2 contains two actin-binding sites (the motor domain and the TH2 domain) and is able to crosslink actin filaments to form bundles. Based on above results, we propose a positive-feedback model explaining FgMyo1-dependent actin polymerization at endocytic site in subapical hyphae of F. graminearum: FgMyo1 molecules anchor at bottom of endocytic pitch, driving inward movement of actin filaments and enhancing actin polymerization; with more actin filaments are formed, more FgMyo1 molecules are recruited to the endocytic site.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107841"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonthermal plasma jet mitigates viral replication and inflammation in human coronavirus 229E-infected lung cells by targeting the NF-κB and MAPK pathways.","authors":"Khadija Akter, Young June Hong, Ihn Han, Eun Ha Choi","doi":"10.1016/j.micpath.2025.107838","DOIUrl":"10.1016/j.micpath.2025.107838","url":null,"abstract":"<p><p>SARS-CoV-2 is a pressing global health issue, largely driven by uncontrolled viral replication and severe proinflammatory responses. Despite significant technological progress made by humanity, medical science has frequently found itself incapable of effectively addressing pathogenic outbreaks. Nonthermal plasma is a promising technology for combating pathogenic microorganisms. Considering the pressing demand for new treatment options, we have investigated the prospects of plasma-treated medium (PTM) enriched with reactive oxygen and nitrogen species (ROS/RNS) as a promising approach to combat human corona virus (HCoV-229E). The PTM was prepared using nonthermal plasma jet (NTPJ) with air as a flowing gas. NTPJ treatment effectively reduced the viral infection in MRC-5 lung cells, as evidenced through the assessment of the cytopathogenic effects. The mRNA expression of the viral gene ACE-2, Spike (S) gene, Rd/Rp Helicase gene and the generation of inflammation markers, IL-6, TNF-α, IL-1β, IFN-γ,COX-2,NF-κB, CXCL-10, and CCl-5 were significantly reduced with NTPJ. Moreover, confocal microscopy analysis revealed a significant reduction in spike glycoprotein and nucleocapsid protein levels in the cytoplasm. This finding was further supported by flow cytometry analysis, which confirmed a significant decrease in spike glycoprotein levels. Furthermore, mechanism studies indicated that NTPJ may influence cellular processes in HCoV-229E infected lung cells by disrupting the NF-κB and MAPK pathways, particularly evidenced by changes in phosphorylation levels. The pretreatment of the virus with SP600125 inhibitor and NTPJ resulted in a significant reduction in the expression ratio of viral genes ACE-2, S gene and RdRP/Helicase. Therefore, these results suggest that NTPJ enhances both anti-viral and anti-inflammatory responses, making it a promising candidate for prevention and treatment of corona virus infections.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107838"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis.","authors":"María F Cerioli, Franco D Fernández, Melina V Moliva, Federico Serral, Darío Fernandez Do Porto, Elina B Reinoso","doi":"10.1016/j.micpath.2025.107846","DOIUrl":"10.1016/j.micpath.2025.107846","url":null,"abstract":"<p><p>Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore platforms. Final genome was assembled in a single circular chromosome and one plasmid comprising 2.556.630 bp (GC% 38.60) and 150.514 bp (GC 36 %), respectively. Phylogeny confirmed that strain SU-B46 is closely related to E. lactis reference genomes. The alignment confirmed the presence of E. lactis. Additionally, the genomic analysis identified twelve potential virulence genes related to adhesion, immune evasion and biofilm formation among others, but none were found in the plasmid. The strain exhibited resistance to aminoglycosides, macrolides and glycopeptides. This study presents the first report of an E. lactis strain isolated from a subclinical mastitis case in Argentina, broadening the geographical and host range of this pathogen. These findings suggest the possible involvement of E. lactis as an etiological agent of bovine mastitis. Further studies are needed to understand the significance of this agent, warranting additional investigation to ensure its optimal management, especially within the dairy industry.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107846"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis viruses hijack cellular metabolic pathways to drive replication and disease: Implications for novel therapeutics.","authors":"Nanqin Peng, Qingyan Lin, Xiaotian Huang, Xiaomin Yu","doi":"10.1016/j.micpath.2025.107837","DOIUrl":"10.1016/j.micpath.2025.107837","url":null,"abstract":"<p><p>Hepatitis viruses are one of the most common viruses threatening humans all over the world, which can lead to viral hepatitis and even increase the risk of hepatocellular carcinoma. Numerous investigations have shown that hepatitis viruses reprogram the metabolisms of infected cells, including alterations in glucose, amino acid, and lipid metabolic pathways, resulting in changes of the life cycles of viruses, the status of host cells and the development of diseases. In this review, we summarized how hepatitis viruses rewire cells' metabolisms to promote virus replication and pathogenesis, which contributes to the exploitation of potential therapeutic paradigms.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107837"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted inhibition of Candida albicans by red alga Palisada cruciata (Harvey) K.W. Nam (2007): A new report on anticandidal and antioxidant potential with phytochemical analysis using GC-MS and FT-IR study.","authors":"Vigneshkumar Venkatesan, Antony Prakash Rejoy Patrick, Nagaraj Subramani","doi":"10.1016/j.micpath.2025.107852","DOIUrl":"10.1016/j.micpath.2025.107852","url":null,"abstract":"<p><p>The biotechnological potential of marine red seaweed remains an active area of research against microbial infections caused by fungal pathogens. In this study, the methanolic extract of Palisada cruciata. was evaluated for its efficacy in anti-candidal and antioxidant activities, highlighting its potential as a source of bioactive compounds from an unexplored species of P. cruciata, which adds valuable information against Candida infections. The extract demonstrated significant antifungal activity against Candida albicans strains C11 and C13, with inhibition zones of 11 mm and 10 mm at 1 mg/mL, respectively, outperforming extracts from Sargassum wightii and Caulerpa scalpelliformis. Antioxidant assays further confirmed its bioactivity, with 66.88 ± 1.8 % DPPH radical scavenging, 62.5 ± 2.0 % hydroxyl radical scavenging, and a total antioxidant capacity of 68.83 ± 2.8 μg AAE/g. Phytochemical screening confirms the presence of secondary metabolites such as terpenoids, phenols, and sterols, which are known contributors to antimicrobial and antioxidant properties. FT-IR analysis revealed key functional groups, including alkanes, amines, and aromatic compounds, while GC-MS detected major constituents such as n-Hexadecenoic acid (62.16 %), 2-Pentadecanone (10.94 %), and Hexadecanoic acid methyl ester (8.72 %), alongside bioactive hydrocarbons and long-chain alcohols. Thin-layer chromatography further demonstrated the extract's chemical complexity and diversity. Given the rising concern over antifungal resistance, P. cruciata. presents a promising candidate for the development of novel anti-candidal agents. This study underscores the significance of marine-derived bioactive in pharmaceutical applications, advocating for further isolation, structural characterization, and mechanistic studies. Expanding research into marine natural products can contribute to sustainable drug discovery, addressing the growing need for alternative antifungal therapies.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107852"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clustering analysis of antibiotic resistance in multidrug-resistant bacteria from spoiled vegetables.","authors":"Emad Abada, Abdullah Mashraqi, Yosra Modafer, Shifaa O Alshammari","doi":"10.1016/j.micpath.2025.107819","DOIUrl":"10.1016/j.micpath.2025.107819","url":null,"abstract":"<p><p>Antibiotic resistance in foodborne bacteria is a growing public health concern, particularly in fresh produce. This study evaluates multidrug-resistant bacteria (MDRB) isolated from spoiled zucchini, cucumber, and potato collected from Sabia, Abu Arish, and Samta in the Jazan region. Bacterial isolates were cultured on MacConkey and Mannitol salt agar, followed by Gram staining to identify three Gram-negative (KMcS, CMcA, PMcSa) and three Gram-positive (KMS, CMA, PMSa) isolates. Antibiotic susceptibility testing was performed against Penicillin (P), Streptomycin (S), Cefotaxime (CTX), Chloramphenicol (C), Ofloxacin (OF), and Erythromycin (E). The inhibition zones varied across antibiotics, with Ofloxacin exhibiting the largest inhibition zones (40-50 mm) and Cefotaxime and Erythromycin showing the weakest inhibition (≤14 mm). The Resistance Index (RI), which represents the proportion of antibiotics with no inhibition, ranged from 0.2 (moderate resistance) to 0.6 (high resistance in the PMcSa isolate). ANOVA (p ≈ 6.95e-08) confirmed significant differences in inhibition zones across antibiotics, while Tukey's HSD test showed that Ofloxacin was significantly more effective than both Cefotaxime (p = 0.0005) and Erythromycin (p = 0.0035). Correlation analysis revealed strong positive associations between resistance to Chloramphenicol, Streptomycin, and Ofloxacin (r > 0.7), suggesting potential cross-resistance mechanisms. Hierarchical clustering and KMeans analysis (K = 3) grouped bacterial isolates based on their resistance patterns, while PCA visualization distinguished Ofloxacin-sensitive and multidrug-resistant isolates. The findings highlight an urgent need for targeted antibiotic stewardship programs, especially in agricultural practices, to limit the overuse of antibiotics. Additionally, enhanced sanitation measures during food handling and real-time monitoring of bacterial contamination in the food supply chain could significantly reduce the spread of antibiotic-resistant bacteria from farm to table.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107819"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Withaferin A targets the membrane of Pseudomonas aeruginosa and mitigates the inflammation in zebrafish larvae; an in vitro and in vivo approach\" [Microb. Pathog. 172 (2022) 105778].","authors":"Raghul Murugan, Ravi Rajesh, Boopathi Seenivasan, B Haridevamuthu, Gokul Sudhakaran, Ajay Guru, Rajakrishnan Rajagopal, Palaniselvam Kuppusamy, Annie Juliet, Pushparathinam Gopinath, Jesu Arockiaraj","doi":"10.1016/j.micpath.2025.107845","DOIUrl":"10.1016/j.micpath.2025.107845","url":null,"abstract":"","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107845"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective mechanism of Bifidobacterium on intestinal mucosal barrier in sepsis.","authors":"Kai-Jun Zhang, Yi-Feng Chang, Yi-Fan Liu, Shi-Xue Dai, Jie Li","doi":"10.1016/j.micpath.2025.107785","DOIUrl":"10.1016/j.micpath.2025.107785","url":null,"abstract":"<p><p>Prior research has examined the advantageous impacts of probiotics in preventing and treating sepsis; however, the protective actions of Bifidobacterium against sepsis are still not understood. In this review, we innovatively discussed the mechanisms by which Bifidobacterium can prevent and treat sepsis by regulating the intestinal mucosal barrier during homeostasis and when damaged. Surprisingly, we found that the mechanisms include blocking NF-κB and p38 MAPK signaling pathways, regulating AHR/NRF2/NLRP3 inflammasome pathways, inhibiting NLRP3/ASC/Caspase1 signaling pathways, regulating enteric glial cells (EGCs) network development, regulating immunity, and restoring intestinal symbiosis, etc. Through continued pathway researches into mechanisms of probiotics on sepsis, we try to get more evidence of Bifidobacterium on the prevention and treatment of sepsis.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107785"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable management of southern blight in peanut using volatile organic compounds produced by Bacillus amyloliquefaciens HK17.","authors":"Seo Hyun Hwang, Jun Su Noh, Chaw Ei Htwe Maung, Kil Yong Kim","doi":"10.1016/j.micpath.2025.107829","DOIUrl":"10.1016/j.micpath.2025.107829","url":null,"abstract":"<p><p>Southern blight, caused by Sclerotium rolfsii, is a highly destructive soilborne fungal disease that severely impacts peanut production worldwide. The rapid spread and aggressive nature of this pathogen, coupled with its ability to produce sclerotia that can persist in soil for extended periods, make it a significant threat to global agriculture. This study explored the biocontrol potential of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens HK17 as an alternative approach to managing southern blight in peanut. VOCs produced by strain HK17 in two different growth media-tryptic soy broth medium (TSB-HK17) and sterile compost-amended soil (SCS-HK17)-effectively inhibited the mycelial growth and sclerotia germination of S. rolfsii and disrupted the synthesis of oxalic acid, a key virulence factor of S. rolfsii. They also caused a significant reduction in the activity of plant cell wall-degrading enzymes associated with fungal pathogenicity. Gas chromatography-mass spectrometry analysis showed the VOCs belonged to several classes of compounds, including alcohols, amines, and sulfur-containing compounds, with amines being particularly dominant. Notably, 5-nonylamine was the most effective in reducing the growth of mycelia from both mycelial plugs and germinated sclerotia. Treatment with TSB-HK17- and SCS-HK17-produced VOCs significantly controlled S. rolfsii invasion in peanut plants compared to untreated controls. These results indicate that VOCs produced by B. amyloliquefaciens HK17 could be utilized as promising, environmentally friendly agents for the effective management of southern blight in peanut and other crops, offering a sustainable alternative to traditional chemical pesticides.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107829"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}