Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis.

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-01 Epub Date: 2025-06-27 DOI:10.1016/j.micpath.2025.107846

María F Cerioli, Franco D Fernández, Melina V Moliva, Federico Serral, Darío Fernandez Do Porto, Elina B Reinoso

{"title":"Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis.","authors":"María F Cerioli, Franco D Fernández, Melina V Moliva, Federico Serral, Darío Fernandez Do Porto, Elina B Reinoso","doi":"10.1016/j.micpath.2025.107846","DOIUrl":null,"url":null,"abstract":"<p><p>Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore platforms. Final genome was assembled in a single circular chromosome and one plasmid comprising 2.556.630 bp (GC% 38.60) and 150.514 bp (GC 36 %), respectively. Phylogeny confirmed that strain SU-B46 is closely related to E. lactis reference genomes. The alignment confirmed the presence of E. lactis. Additionally, the genomic analysis identified twelve potential virulence genes related to adhesion, immune evasion and biofilm formation among others, but none were found in the plasmid. The strain exhibited resistance to aminoglycosides, macrolides and glycopeptides. This study presents the first report of an E. lactis strain isolated from a subclinical mastitis case in Argentina, broadening the geographical and host range of this pathogen. These findings suggest the possible involvement of E. lactis as an etiological agent of bovine mastitis. Further studies are needed to understand the significance of this agent, warranting additional investigation to ensure its optimal management, especially within the dairy industry.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107846"},"PeriodicalIF":3.3000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial pathogenesis","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.micpath.2025.107846","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/27 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore platforms. Final genome was assembled in a single circular chromosome and one plasmid comprising 2.556.630 bp (GC% 38.60) and 150.514 bp (GC 36 %), respectively. Phylogeny confirmed that strain SU-B46 is closely related to E. lactis reference genomes. The alignment confirmed the presence of E. lactis. Additionally, the genomic analysis identified twelve potential virulence genes related to adhesion, immune evasion and biofilm formation among others, but none were found in the plasmid. The strain exhibited resistance to aminoglycosides, macrolides and glycopeptides. This study presents the first report of an E. lactis strain isolated from a subclinical mastitis case in Argentina, broadening the geographical and host range of this pathogen. These findings suggest the possible involvement of E. lactis as an etiological agent of bovine mastitis. Further studies are needed to understand the significance of this agent, warranting additional investigation to ensure its optimal management, especially within the dairy industry.

查看原文本刊更多论文

牛亚临床乳腺炎乳糜球菌分离株的基因组分析。

环境细菌，如链球菌和肠球菌，在牛乳腺炎中起重要作用。本研究的目的是表征从一例亚临床乳腺炎分离肠球菌的基因组序列。利用Illumina和Oxford Nanopore平台分离基因组DNA并进行测序。最终基因组组装在一条环状染色体和一个质粒上，分别为2.556.630 bp （GC% 38.60）和150.514 bp （GC% 36%）。系统发育证实菌株SU-B46与乳杆菌参考基因组亲缘关系密切。排列结果证实了E. lactis的存在。此外，基因组分析发现了12个与粘附、免疫逃避和生物膜形成等相关的潜在毒力基因，但在质粒中没有发现。该菌株对氨基糖苷类、大环内酯类和糖肽具有抗性。本研究首次报道从阿根廷亚临床乳腺炎病例中分离出乳杆菌菌株，拓宽了该病原体的地理和宿主范围。这些发现提示乳杆菌可能作为牛乳腺炎的病原。需要进一步的研究来了解该制剂的重要性，需要进一步的调查以确保其最佳管理，特别是在乳制品行业。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)