First isolation and characterization of caprine parainfluenza virus type 3 from cattle in China

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-22 DOI:10.1016/j.micpath.2025.108061

Yao Zhang , Kaicheng Wang , Youyi Lu , Xuwen Li , Wenliang Li , Zihao Pan , Huochun Yao

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引用次数: 0

Abstract

Caprine parainfluenza virus type 3 (CPIV3) is an enveloped single-stranded negative-sense RNA virus, commonly found in respiratory tract infections of goats and sheep. However, there have been no reports of CPIV3 infection in cattle. In this study, a strain of CPIV3 named HB2304S3 was isolated from a cattle with respiratory symptoms using Madin-Darby bovine kidney cells. The strain was verified by PCR and high-throughput sequencing, and the complete genomic sequence was obtained. After purification of the virus, hemagglutination tests and physicochemical property tests were conducted on this CPIV3 strain. The hemagglutination titer of the isolate was 1:40, and it was sensitive to ether, trypsin, temperature, and pH. To further investigate CPIV3 epidemiology in Chinese cattle populations, we conducted molecular surveillance across multiple provinces. The results revealed that 52 samples were positive for CPIV3, with a total positive rate of 34.21 % (52/152). Moreover, CPIV3 was detected in 12 cattle farms, with a farm positive rate of 70.59 % (12/17). These findings provide the first conclusive evidence of CPIV3 cross-species transmission to cattle and reveal its significant prevalence within cattle populations in China.

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中国首次从牛中分离出3型山羊副流感病毒并进行鉴定。

3型山羊副流感病毒（CPIV3）是一种包膜单链负义RNA病毒，常见于山羊和绵羊的呼吸道感染。然而，没有牛感染CPIV3的报告。本研究利用Madin-Darby牛肾细胞从有呼吸道症状的牛中分离出一株名为HB2304S3的CPIV3病毒。通过PCR和高通量测序对菌株进行验证，获得了完整的基因组序列。纯化病毒后，对该CPIV3株进行血凝试验和理化性质试验。该分离株血凝滴度为1:40，对乙醚、胰蛋白酶、温度和ph敏感。为了进一步了解CPIV3在中国牛种群中的流行病学，我们在多个省份进行了分子监测。结果52份标本CPIV3阳性，总阳性率为34.21%（52/152）。12个牛场检测到CPIV3，牧场阳性率为70.59%（12/17）。这些发现首次提供了CPIV3跨物种传播给牛的确凿证据，并揭示了该病毒在中国牛种群中的显著流行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)