Microbial pathogenesis最新文献

{"title":"Comparative profiling of splenic phospholipid and sphingolipid during acute infection","authors":"Tao Liu ,&nbsp;Hong Jia ,&nbsp;Hui Dong ,&nbsp;Chaofei Cheng ,&nbsp;Tao Zhang ,&nbsp;Xinghui Zhao ,&nbsp;Zhanzhong Zhao","doi":"10.1016/j.micpath.2025.108015","DOIUrl":"10.1016/j.micpath.2025.108015","url":null,"abstract":"<div><div>Lipid profile of spleen and bursa of Fabricius (BF) during acute infection remains unknown. Acute infection models of porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and <em>Eimeria tenella</em> (ET) were developed, and spleen samples with African swine fever virus (ASFV) or not were collected. Spleen and BF were examined and characteristic microscopic lesions were observed. Samples were analyzed using a semi-quantitative, untargeted method based on liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS-MS). After statistical analysis, potential biomarkers for PRRSV [16:0 20:5 phosphatidylcholine (16:0 20:5 PC), 18:1 lysophosphatidylcholine (18:1 LPC)], for PEDV [16:0 20:4 phosphatidylinositol (16:0 20:4 PI), 18:0 18:2 phosphatidylserine (18:0 18:2 PS), 16:0 lysophosphatidylserine (16:0 LysoPS), 18:0 LysoPS, d18:1 16:0 sphingomyelin (d18:1 16:0 SM)], for ASFV [16:0 18:0 PC, 16:0 20:4 PC, 18:0 20:4 PC, O-16:0 20:4 PC, O-18:1 20:4 PC, 16:0 18:2 phosphatidylethanolamine (16:0 18:2 PE), 16:0 18:1 PE, 18:0 20:4 PE, 16:0 18:2 PI, 18:0 18:1 PS], for ET with spleen [16:0 20:3 PC, 18:0 18:2 PC, 18:0 18:1 PI, 18:0 20:4 PI, 18:0 22:6 PI, d18:1 16:0 SM, d18:1 22:1 SM], for ET with BF [16:0 18:2 PI, 16:0 18:1 PI, 18:0 18:2 PI, 18:0 20:4 PI, 16:0 18:2 PS, 16:0 18:1 PS, 18:0 18:2 PS, 18:0 20:4 PS, 18:0 22:6 PS, 18:0 LysoPS, 20:4 arachidonic acid (20:4 AA), 22:6 docosahexaenoic acid (22:6 DHA)] were analyzed. Overall, lipid profiling of spleen and BF was demonstrated, contributing to understand the lipid biology of lymphoid organs and intervene acute infection.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"208 ","pages":"Article 108015"},"PeriodicalIF":3.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis.","authors":"María F Cerioli, Franco D Fernández, Melina V Moliva, Federico Serral, Darío Fernandez Do Porto, Elina B Reinoso","doi":"10.1016/j.micpath.2025.107846","DOIUrl":"10.1016/j.micpath.2025.107846","url":null,"abstract":"<p><p>Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore platforms. Final genome was assembled in a single circular chromosome and one plasmid comprising 2.556.630 bp (GC% 38.60) and 150.514 bp (GC 36 %), respectively. Phylogeny confirmed that strain SU-B46 is closely related to E. lactis reference genomes. The alignment confirmed the presence of E. lactis. Additionally, the genomic analysis identified twelve potential virulence genes related to adhesion, immune evasion and biofilm formation among others, but none were found in the plasmid. The strain exhibited resistance to aminoglycosides, macrolides and glycopeptides. This study presents the first report of an E. lactis strain isolated from a subclinical mastitis case in Argentina, broadening the geographical and host range of this pathogen. These findings suggest the possible involvement of E. lactis as an etiological agent of bovine mastitis. Further studies are needed to understand the significance of this agent, warranting additional investigation to ensure its optimal management, especially within the dairy industry.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107846"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis viruses hijack cellular metabolic pathways to drive replication and disease: Implications for novel therapeutics.","authors":"Nanqin Peng, Qingyan Lin, Xiaotian Huang, Xiaomin Yu","doi":"10.1016/j.micpath.2025.107837","DOIUrl":"10.1016/j.micpath.2025.107837","url":null,"abstract":"<p><p>Hepatitis viruses are one of the most common viruses threatening humans all over the world, which can lead to viral hepatitis and even increase the risk of hepatocellular carcinoma. Numerous investigations have shown that hepatitis viruses reprogram the metabolisms of infected cells, including alterations in glucose, amino acid, and lipid metabolic pathways, resulting in changes of the life cycles of viruses, the status of host cells and the development of diseases. In this review, we summarized how hepatitis viruses rewire cells' metabolisms to promote virus replication and pathogenesis, which contributes to the exploitation of potential therapeutic paradigms.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107837"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted inhibition of Candida albicans by red alga Palisada cruciata (Harvey) K.W. Nam (2007): A new report on anticandidal and antioxidant potential with phytochemical analysis using GC-MS and FT-IR study.","authors":"Vigneshkumar Venkatesan, Antony Prakash Rejoy Patrick, Nagaraj Subramani","doi":"10.1016/j.micpath.2025.107852","DOIUrl":"10.1016/j.micpath.2025.107852","url":null,"abstract":"<p><p>The biotechnological potential of marine red seaweed remains an active area of research against microbial infections caused by fungal pathogens. In this study, the methanolic extract of Palisada cruciata. was evaluated for its efficacy in anti-candidal and antioxidant activities, highlighting its potential as a source of bioactive compounds from an unexplored species of P. cruciata, which adds valuable information against Candida infections. The extract demonstrated significant antifungal activity against Candida albicans strains C11 and C13, with inhibition zones of 11 mm and 10 mm at 1 mg/mL, respectively, outperforming extracts from Sargassum wightii and Caulerpa scalpelliformis. Antioxidant assays further confirmed its bioactivity, with 66.88 ± 1.8 % DPPH radical scavenging, 62.5 ± 2.0 % hydroxyl radical scavenging, and a total antioxidant capacity of 68.83 ± 2.8 μg AAE/g. Phytochemical screening confirms the presence of secondary metabolites such as terpenoids, phenols, and sterols, which are known contributors to antimicrobial and antioxidant properties. FT-IR analysis revealed key functional groups, including alkanes, amines, and aromatic compounds, while GC-MS detected major constituents such as n-Hexadecenoic acid (62.16 %), 2-Pentadecanone (10.94 %), and Hexadecanoic acid methyl ester (8.72 %), alongside bioactive hydrocarbons and long-chain alcohols. Thin-layer chromatography further demonstrated the extract's chemical complexity and diversity. Given the rising concern over antifungal resistance, P. cruciata. presents a promising candidate for the development of novel anti-candidal agents. This study underscores the significance of marine-derived bioactive in pharmaceutical applications, advocating for further isolation, structural characterization, and mechanistic studies. Expanding research into marine natural products can contribute to sustainable drug discovery, addressing the growing need for alternative antifungal therapies.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107852"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clustering analysis of antibiotic resistance in multidrug-resistant bacteria from spoiled vegetables.","authors":"Emad Abada, Abdullah Mashraqi, Yosra Modafer, Shifaa O Alshammari","doi":"10.1016/j.micpath.2025.107819","DOIUrl":"10.1016/j.micpath.2025.107819","url":null,"abstract":"<p><p>Antibiotic resistance in foodborne bacteria is a growing public health concern, particularly in fresh produce. This study evaluates multidrug-resistant bacteria (MDRB) isolated from spoiled zucchini, cucumber, and potato collected from Sabia, Abu Arish, and Samta in the Jazan region. Bacterial isolates were cultured on MacConkey and Mannitol salt agar, followed by Gram staining to identify three Gram-negative (KMcS, CMcA, PMcSa) and three Gram-positive (KMS, CMA, PMSa) isolates. Antibiotic susceptibility testing was performed against Penicillin (P), Streptomycin (S), Cefotaxime (CTX), Chloramphenicol (C), Ofloxacin (OF), and Erythromycin (E). The inhibition zones varied across antibiotics, with Ofloxacin exhibiting the largest inhibition zones (40-50 mm) and Cefotaxime and Erythromycin showing the weakest inhibition (≤14 mm). The Resistance Index (RI), which represents the proportion of antibiotics with no inhibition, ranged from 0.2 (moderate resistance) to 0.6 (high resistance in the PMcSa isolate). ANOVA (p ≈ 6.95e-08) confirmed significant differences in inhibition zones across antibiotics, while Tukey's HSD test showed that Ofloxacin was significantly more effective than both Cefotaxime (p = 0.0005) and Erythromycin (p = 0.0035). Correlation analysis revealed strong positive associations between resistance to Chloramphenicol, Streptomycin, and Ofloxacin (r > 0.7), suggesting potential cross-resistance mechanisms. Hierarchical clustering and KMeans analysis (K = 3) grouped bacterial isolates based on their resistance patterns, while PCA visualization distinguished Ofloxacin-sensitive and multidrug-resistant isolates. The findings highlight an urgent need for targeted antibiotic stewardship programs, especially in agricultural practices, to limit the overuse of antibiotics. Additionally, enhanced sanitation measures during food handling and real-time monitoring of bacterial contamination in the food supply chain could significantly reduce the spread of antibiotic-resistant bacteria from farm to table.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107819"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Withaferin A targets the membrane of Pseudomonas aeruginosa and mitigates the inflammation in zebrafish larvae; an in vitro and in vivo approach\" [Microb. Pathog. 172 (2022) 105778].","authors":"Raghul Murugan, Ravi Rajesh, Boopathi Seenivasan, B Haridevamuthu, Gokul Sudhakaran, Ajay Guru, Rajakrishnan Rajagopal, Palaniselvam Kuppusamy, Annie Juliet, Pushparathinam Gopinath, Jesu Arockiaraj","doi":"10.1016/j.micpath.2025.107845","DOIUrl":"10.1016/j.micpath.2025.107845","url":null,"abstract":"","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107845"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective mechanism of Bifidobacterium on intestinal mucosal barrier in sepsis.","authors":"Kai-Jun Zhang, Yi-Feng Chang, Yi-Fan Liu, Shi-Xue Dai, Jie Li","doi":"10.1016/j.micpath.2025.107785","DOIUrl":"10.1016/j.micpath.2025.107785","url":null,"abstract":"<p><p>Prior research has examined the advantageous impacts of probiotics in preventing and treating sepsis; however, the protective actions of Bifidobacterium against sepsis are still not understood. In this review, we innovatively discussed the mechanisms by which Bifidobacterium can prevent and treat sepsis by regulating the intestinal mucosal barrier during homeostasis and when damaged. Surprisingly, we found that the mechanisms include blocking NF-κB and p38 MAPK signaling pathways, regulating AHR/NRF2/NLRP3 inflammasome pathways, inhibiting NLRP3/ASC/Caspase1 signaling pathways, regulating enteric glial cells (EGCs) network development, regulating immunity, and restoring intestinal symbiosis, etc. Through continued pathway researches into mechanisms of probiotics on sepsis, we try to get more evidence of Bifidobacterium on the prevention and treatment of sepsis.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107785"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable management of southern blight in peanut using volatile organic compounds produced by Bacillus amyloliquefaciens HK17.","authors":"Seo Hyun Hwang, Jun Su Noh, Chaw Ei Htwe Maung, Kil Yong Kim","doi":"10.1016/j.micpath.2025.107829","DOIUrl":"10.1016/j.micpath.2025.107829","url":null,"abstract":"<p><p>Southern blight, caused by Sclerotium rolfsii, is a highly destructive soilborne fungal disease that severely impacts peanut production worldwide. The rapid spread and aggressive nature of this pathogen, coupled with its ability to produce sclerotia that can persist in soil for extended periods, make it a significant threat to global agriculture. This study explored the biocontrol potential of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens HK17 as an alternative approach to managing southern blight in peanut. VOCs produced by strain HK17 in two different growth media-tryptic soy broth medium (TSB-HK17) and sterile compost-amended soil (SCS-HK17)-effectively inhibited the mycelial growth and sclerotia germination of S. rolfsii and disrupted the synthesis of oxalic acid, a key virulence factor of S. rolfsii. They also caused a significant reduction in the activity of plant cell wall-degrading enzymes associated with fungal pathogenicity. Gas chromatography-mass spectrometry analysis showed the VOCs belonged to several classes of compounds, including alcohols, amines, and sulfur-containing compounds, with amines being particularly dominant. Notably, 5-nonylamine was the most effective in reducing the growth of mycelia from both mycelial plugs and germinated sclerotia. Treatment with TSB-HK17- and SCS-HK17-produced VOCs significantly controlled S. rolfsii invasion in peanut plants compared to untreated controls. These results indicate that VOCs produced by B. amyloliquefaciens HK17 could be utilized as promising, environmentally friendly agents for the effective management of southern blight in peanut and other crops, offering a sustainable alternative to traditional chemical pesticides.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107829"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Glucans from Saccharomyces cerevisiae as antiadhesive and immunomodulating polysaccharides against Campylobacterjejuni.","authors":"L Kemper, A Hensel","doi":"10.1016/j.micpath.2025.107847","DOIUrl":"10.1016/j.micpath.2025.107847","url":null,"abstract":"<p><p>Campylobacter jejuni is responsible for the majority of food-borne enteritis worldwide with severe cases of diarrhea and being the third leading cause of death globally. The bacterium strongly colonizes the intestine of poultry without affecting the animals, but leading to severe infections in humans after oral intake of contaminated eggs or undercooked meat. A commercially available β-1,3/1,6-glucan from Saccharomyces cerevisiae (BGL) was shown to interact with early host-pathogen interactions of C. jejuni to eucaryotic host cells. BGL with a β-1,3-D-glucose backbone and short oligosaccharide glucose side chains (DP4 to 6) at position O-6 of the backbone reduced C. jejuni adhesion to human intestinal Caco-2 cells in a concentration dependent manner (1-1000 μg/mL) up to 45 % within coincubation protocol. Preincubation of either bacteria or host cells with BGL indicated that C. jejuni is significantly impaired in its capability to recognize and to attach to eukaryotic host cells. In contrast, adhesion of untreated C. jejuni to BGL-pretreated host cells remains unchanged. Cellular viability of host cells and proliferation of the bacteria are not significantly influenced by BGL. Beside reduced adhesion to Caco-2 cells, BGL treatment of RAW 264.7 macrophages significantly stimulated phagocytosis of fluorescent-labeled zymosan particles as well as internalization of C. jejuni. Interestingly, BGL reduced also the bacterial adhesion to the macrophages, but phagocytosis of the bacteria is strongly stimulated. The observed effects of yeast glucans could prove beneficial for protecting intestinal cells from C. jejuni infection by inhibition of bacterial adhesion and by increased and faster elimination.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107847"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First detection of CTX-M-65 ESBL gene in Morocco: Evidence of transcontinental transmission linked to commercial exchange.","authors":"Oumaima Fazza, Bouchra Karraouane, Germán Kopprio, Brahim Bouchrif, Houda Ennassiri, Mireille Favard Ennachachibi, Abdelaziz Hmyene","doi":"10.1016/j.micpath.2025.107855","DOIUrl":"10.1016/j.micpath.2025.107855","url":null,"abstract":"<p><strong>Introduction: </strong>The study aimed to determine the occurrence of genes encoding antibacterial resistance in Escherichia coli (E. coli) isolates recovered from poultry products.</p><p><strong>Method: </strong>ology: Antibiotic susceptibility was tested using the disc diffusion method, and then resistance genes and virulence profiles were determined by whole-genome analysis.</p><p><strong>Results: </strong>Out of 154 poultry products, 96 (62.34 %) were E. coli. Two isolates were positive to the test of synergy between the clavulanic acid and the third generation of cephalosporin (C3G) and showed a high level of resistance to penicillins and fluoroquinolones. Besides, the isolate E2 was notably resistant to tobramycine, tigecycline, aztreonam, and chloramphenicol. Whole-genome screening showed the presence of ESBL genes, namely, blaCTX-M-65, blaEC-15, blaTEM-1, and quinolone resistance genes gyrA_S83L and parC_S80I, in addition to other resistance genes. The virulence profile did not show the presence of either of the Shiga toxin genes stx1 and sxt2; nevertheless, other virulence factors were detected, such as cvaC; responsible for the production of colicin V and iroABCDEN genes.</p><p><strong>Conclusion: </strong>The results alert us about the hygienic state of poultry products and the increasing level of antibacterial resistance, and pose a potential risk that could be caused by the transmission of the antimicrobial resistance to humans through the consumption of food products, leading to a reduction of the available antibiotic treatment options.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107855"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}