{"title":"利用16S rRNA测序分析益生菌对腹泻型穿山甲肠道菌群的影响","authors":"Lina Zhang , Qihui Li , Yan Hua","doi":"10.1016/j.micpath.2025.108036","DOIUrl":null,"url":null,"abstract":"<div><div>The gut microbiota plays important roles in intestinal health, but the changes and functions of microbiota in diarrheal pangolins remain poorly understood. This study analyzed gut microbiota changes in 12 Chinese pangolins (<em>Manis pentadactyla</em>) from diarrhea to recovery using <em>16S rRNA</em> amplicon sequencing. Fecal samples of Chinese pangolins were achieved at day 1 (diarrhea) and day 5 (post-probiotic treatment). Results showed significantly increased microbial diversity and structural shifts in gut microbiota following probiotics treatment. A total of 18 phyla were identified in the fecal microorganisms of Chinese pangolins. <em>Firmicutes</em>, <em>Proteobacteria</em>, <em>Bacteroidetes</em>, and <em>Fusobacteria</em> were predominant bacteria. At the genus level, <em>Clostridium_sensu_stricto_1</em>, <em>Bacteroides</em>, <em>Clostridium</em>, <em>Streptococcus</em>, <em>Escherichia-Shigella</em>, <em>Megasphaera</em>, <em>Cellulosilyticum,</em> and <em>Lactococcus</em> were the predominant in both diarrheal groups and recovery group. After probiotic treatment, <em>Streptococcus</em> (<em>P</em> = 0.00001), and <em>Escherichia-Shigella</em> (<em>P</em> = 0.04) were significantly decreased<em>,</em> and similar phenomenon was observed in <em>Limosilactobacillus</em> (<em>P</em> = 0.0003) and <em>Lactobacillus</em> (<em>P</em> = 0.0002). In conclusion, this study provides a comprehensive analysis of gut microbiota shifts during diarrhea treatment in Chinese pangolins, indicating that probiotic agents could be an adjuvant therapy when treating diarrhea in captive pangolins.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"209 ","pages":"Article 108036"},"PeriodicalIF":3.5000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of probiotics on gut microbiota in diarrheal Chinese pangolins (Manis pentadactyla) analyzed through 16S rRNA sequencing\",\"authors\":\"Lina Zhang , Qihui Li , Yan Hua\",\"doi\":\"10.1016/j.micpath.2025.108036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The gut microbiota plays important roles in intestinal health, but the changes and functions of microbiota in diarrheal pangolins remain poorly understood. This study analyzed gut microbiota changes in 12 Chinese pangolins (<em>Manis pentadactyla</em>) from diarrhea to recovery using <em>16S rRNA</em> amplicon sequencing. Fecal samples of Chinese pangolins were achieved at day 1 (diarrhea) and day 5 (post-probiotic treatment). Results showed significantly increased microbial diversity and structural shifts in gut microbiota following probiotics treatment. A total of 18 phyla were identified in the fecal microorganisms of Chinese pangolins. <em>Firmicutes</em>, <em>Proteobacteria</em>, <em>Bacteroidetes</em>, and <em>Fusobacteria</em> were predominant bacteria. At the genus level, <em>Clostridium_sensu_stricto_1</em>, <em>Bacteroides</em>, <em>Clostridium</em>, <em>Streptococcus</em>, <em>Escherichia-Shigella</em>, <em>Megasphaera</em>, <em>Cellulosilyticum,</em> and <em>Lactococcus</em> were the predominant in both diarrheal groups and recovery group. After probiotic treatment, <em>Streptococcus</em> (<em>P</em> = 0.00001), and <em>Escherichia-Shigella</em> (<em>P</em> = 0.04) were significantly decreased<em>,</em> and similar phenomenon was observed in <em>Limosilactobacillus</em> (<em>P</em> = 0.0003) and <em>Lactobacillus</em> (<em>P</em> = 0.0002). In conclusion, this study provides a comprehensive analysis of gut microbiota shifts during diarrhea treatment in Chinese pangolins, indicating that probiotic agents could be an adjuvant therapy when treating diarrhea in captive pangolins.</div></div>\",\"PeriodicalId\":18599,\"journal\":{\"name\":\"Microbial pathogenesis\",\"volume\":\"209 \",\"pages\":\"Article 108036\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbial pathogenesis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0882401025007612\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial pathogenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0882401025007612","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Effects of probiotics on gut microbiota in diarrheal Chinese pangolins (Manis pentadactyla) analyzed through 16S rRNA sequencing
The gut microbiota plays important roles in intestinal health, but the changes and functions of microbiota in diarrheal pangolins remain poorly understood. This study analyzed gut microbiota changes in 12 Chinese pangolins (Manis pentadactyla) from diarrhea to recovery using 16S rRNA amplicon sequencing. Fecal samples of Chinese pangolins were achieved at day 1 (diarrhea) and day 5 (post-probiotic treatment). Results showed significantly increased microbial diversity and structural shifts in gut microbiota following probiotics treatment. A total of 18 phyla were identified in the fecal microorganisms of Chinese pangolins. Firmicutes, Proteobacteria, Bacteroidetes, and Fusobacteria were predominant bacteria. At the genus level, Clostridium_sensu_stricto_1, Bacteroides, Clostridium, Streptococcus, Escherichia-Shigella, Megasphaera, Cellulosilyticum, and Lactococcus were the predominant in both diarrheal groups and recovery group. After probiotic treatment, Streptococcus (P = 0.00001), and Escherichia-Shigella (P = 0.04) were significantly decreased, and similar phenomenon was observed in Limosilactobacillus (P = 0.0003) and Lactobacillus (P = 0.0002). In conclusion, this study provides a comprehensive analysis of gut microbiota shifts during diarrhea treatment in Chinese pangolins, indicating that probiotic agents could be an adjuvant therapy when treating diarrhea in captive pangolins.
期刊介绍:
Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports.
Research Areas Include:
-Pathogenesis
-Virulence factors
-Host susceptibility or resistance
-Immune mechanisms
-Identification, cloning and sequencing of relevant genes
-Genetic studies
-Viruses, prokaryotic organisms and protozoa
-Microbiota
-Systems biology related to infectious diseases
-Targets for vaccine design (pre-clinical studies)