分子鉴定的白色金蝇蛆提取物的抗菌、抗氧化、伤口愈合和细胞毒作用。

IF 3.5 3区 医学 Q3 IMMUNOLOGY
Ghada E. Dawwam , Abdelwahab A. Ibrahim , Ramy E. El-Ansary , Abeer H.A. Abdelhafiz , Aya S. Omara , Abla D. Abdel-Meguid , Mohamed M. Baz
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引用次数: 0

摘要

由于多药耐药性的发展速度超过了新的有效药物的研究和开发速度,医疗行业目前正面临预防感染的有效疗法短缺的问题。蝇蛆因其药用和治疗潜力而引起了人们的高度关注。本试验研究了白金虫幼虫提取物对革兰氏阳性(单核增生李斯特菌ATCC 19155和金黄色葡萄球菌ATCC 43300)和革兰氏阴性(大肠杆菌ATCC 8739和沙门氏菌ATCC 14028) 4种多重耐药病原菌的抑菌效果。提取物对所有被试细菌均有较强的抑制作用,抑制范围在18 ~ 25 mm之间,对金黄色葡萄球菌的抑制效果最好。此外,提取物具有较强的抗氧化活性,IC50为44.89 μg/mL,对人成纤维细胞(HFB4)细胞高达250 μg/mL无毒。体外伤口愈合试验表明,提取物显著加速伤口愈合在48小时内。GC-MS分析鉴定出17种生物活性化合物,大多数具有已知的抗菌特性。分子对接研究证实了这些化合物与关键细菌蛋白靶点之间稳定的相互作用,支持了观察到的生物活性。作为遗传多样性的一部分,幼虫样本通过线粒体细胞色素c氧化酶亚基I (COXI基因)的核苷酸测序进行鉴定。然后将这些结果与已经存入GenBank的类似序列进行比较。此外,还进行了分子对接研究,以证实和合理化观察到的生物活性。综上所述,这些结果表明白色棘球蚴幼虫提取物具有抗菌、抗氧化和伤口愈合活性,是开发新型药物和治疗药物的安全、天然的候选物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial, antioxidant, wound healing, and cytotoxic effects of maggot extract from molecularly identified Chrysomya albiceps (Diptera: Calliphoridae)
The medical industry is currently experiencing a deficit of effective therapies to prevent infections, as the evolution of multidrug resistance has outpaced the research and development of new effective medications. Blowfly maggots have attracted high interest for their medicinal and therapeutic potential. This study was conducted to assess the antibacterial efficacy of Chrysomya albiceps larval extract against four multidrug-resistant pathogenic bacteria, including Gram-positive (Listeria monocytogenes ATCC 19155 and Staphylococcus aureus ATCC 43300) and Gram-negative bacteria (Escherichia coli ATCC 8739 and Salmonella sp. ATCC 14028). The extract strongly inhibited all tested bacterial species with inhibition zones ranging from 18 to 25 mm, showing the highest effect on S. aureus. Additionally, the extract exhibited potent antioxidant activity with an IC50 of 44.89 μg/mL and was non-toxic to human fibroblast (HFB4) cells up to 250 μg/mL. In vitro wound healing assays demonstrated that the extract significantly accelerated wound closure within 48 h. GC-MS analysis identified 17 bioactive compounds, most with known antimicrobial properties. Molecular docking studies confirmed stable interactions between these compounds and key bacterial protein targets, supporting the observed bioactivity. As part of the genetic diversity, larval samples were identified by nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit I (COXI gene). These results were then compared with similar sequences that had already been put in GenBank. Moreover, a molecular docking study was performed to confirm and rationalize the observed biological activity. Overall, these results suggest that C. albiceps larval extract possesses antibacterial, antioxidant, and wound-healing activities and represents a safe, natural candidate for developing novel pharmaceutical and therapeutic agents.
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来源期刊
Microbial pathogenesis
Microbial pathogenesis 医学-免疫学
CiteScore
7.40
自引率
2.60%
发文量
472
审稿时长
56 days
期刊介绍: Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)
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