Ghada E. Dawwam , Abdelwahab A. Ibrahim , Ramy E. El-Ansary , Abeer H.A. Abdelhafiz , Aya S. Omara , Abla D. Abdel-Meguid , Mohamed M. Baz
{"title":"分子鉴定的白色金蝇蛆提取物的抗菌、抗氧化、伤口愈合和细胞毒作用。","authors":"Ghada E. Dawwam , Abdelwahab A. Ibrahim , Ramy E. El-Ansary , Abeer H.A. Abdelhafiz , Aya S. Omara , Abla D. Abdel-Meguid , Mohamed M. Baz","doi":"10.1016/j.micpath.2025.108056","DOIUrl":null,"url":null,"abstract":"<div><div>The medical industry is currently experiencing a deficit of effective therapies to prevent infections, as the evolution of multidrug resistance has outpaced the research and development of new effective medications. Blowfly maggots have attracted high interest for their medicinal and therapeutic potential. This study was conducted to assess the antibacterial efficacy of <em>Chrysomya albiceps</em> larval extract against four multidrug-resistant pathogenic bacteria, including Gram-positive (<em>Listeria monocytogenes</em> ATCC 19155 and <em>Staphylococcus aureus</em> ATCC 43300) and Gram-negative bacteria (<em>Escherichia coli</em> ATCC 8739 and <em>Salmonella</em> sp. ATCC 14028). The extract strongly inhibited all tested bacterial species with inhibition zones ranging from 18 to 25 mm, showing the highest effect on <em>S. aureus</em>. Additionally, the extract exhibited potent antioxidant activity with an IC<sub>50</sub> of 44.89 μg/mL and was non-toxic to human fibroblast (HFB4) cells up to 250 μg/mL. In vitro wound healing assays demonstrated that the extract significantly accelerated wound closure within 48 h. GC-MS analysis identified 17 bioactive compounds, most with known antimicrobial properties. Molecular docking studies confirmed stable interactions between these compounds and key bacterial protein targets, supporting the observed bioactivity. As part of the genetic diversity, larval samples were identified by nucleotide sequencing of the mitochondrial cytochrome <em>c</em> oxidase subunit I (COXI gene). These results were then compared with similar sequences that had already been put in GenBank. Moreover, a molecular docking study was performed to confirm and rationalize the observed biological activity. Overall, these results suggest that <em>C. albiceps</em> larval extract possesses antibacterial, antioxidant, and wound-healing activities and represents a safe, natural candidate for developing novel pharmaceutical and therapeutic agents.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"209 ","pages":"Article 108056"},"PeriodicalIF":3.5000,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial, antioxidant, wound healing, and cytotoxic effects of maggot extract from molecularly identified Chrysomya albiceps (Diptera: Calliphoridae)\",\"authors\":\"Ghada E. Dawwam , Abdelwahab A. Ibrahim , Ramy E. El-Ansary , Abeer H.A. Abdelhafiz , Aya S. Omara , Abla D. Abdel-Meguid , Mohamed M. Baz\",\"doi\":\"10.1016/j.micpath.2025.108056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The medical industry is currently experiencing a deficit of effective therapies to prevent infections, as the evolution of multidrug resistance has outpaced the research and development of new effective medications. Blowfly maggots have attracted high interest for their medicinal and therapeutic potential. This study was conducted to assess the antibacterial efficacy of <em>Chrysomya albiceps</em> larval extract against four multidrug-resistant pathogenic bacteria, including Gram-positive (<em>Listeria monocytogenes</em> ATCC 19155 and <em>Staphylococcus aureus</em> ATCC 43300) and Gram-negative bacteria (<em>Escherichia coli</em> ATCC 8739 and <em>Salmonella</em> sp. ATCC 14028). The extract strongly inhibited all tested bacterial species with inhibition zones ranging from 18 to 25 mm, showing the highest effect on <em>S. aureus</em>. Additionally, the extract exhibited potent antioxidant activity with an IC<sub>50</sub> of 44.89 μg/mL and was non-toxic to human fibroblast (HFB4) cells up to 250 μg/mL. In vitro wound healing assays demonstrated that the extract significantly accelerated wound closure within 48 h. GC-MS analysis identified 17 bioactive compounds, most with known antimicrobial properties. Molecular docking studies confirmed stable interactions between these compounds and key bacterial protein targets, supporting the observed bioactivity. As part of the genetic diversity, larval samples were identified by nucleotide sequencing of the mitochondrial cytochrome <em>c</em> oxidase subunit I (COXI gene). These results were then compared with similar sequences that had already been put in GenBank. Moreover, a molecular docking study was performed to confirm and rationalize the observed biological activity. Overall, these results suggest that <em>C. albiceps</em> larval extract possesses antibacterial, antioxidant, and wound-healing activities and represents a safe, natural candidate for developing novel pharmaceutical and therapeutic agents.</div></div>\",\"PeriodicalId\":18599,\"journal\":{\"name\":\"Microbial pathogenesis\",\"volume\":\"209 \",\"pages\":\"Article 108056\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbial pathogenesis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0882401025007818\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial pathogenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0882401025007818","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Antimicrobial, antioxidant, wound healing, and cytotoxic effects of maggot extract from molecularly identified Chrysomya albiceps (Diptera: Calliphoridae)
The medical industry is currently experiencing a deficit of effective therapies to prevent infections, as the evolution of multidrug resistance has outpaced the research and development of new effective medications. Blowfly maggots have attracted high interest for their medicinal and therapeutic potential. This study was conducted to assess the antibacterial efficacy of Chrysomya albiceps larval extract against four multidrug-resistant pathogenic bacteria, including Gram-positive (Listeria monocytogenes ATCC 19155 and Staphylococcus aureus ATCC 43300) and Gram-negative bacteria (Escherichia coli ATCC 8739 and Salmonella sp. ATCC 14028). The extract strongly inhibited all tested bacterial species with inhibition zones ranging from 18 to 25 mm, showing the highest effect on S. aureus. Additionally, the extract exhibited potent antioxidant activity with an IC50 of 44.89 μg/mL and was non-toxic to human fibroblast (HFB4) cells up to 250 μg/mL. In vitro wound healing assays demonstrated that the extract significantly accelerated wound closure within 48 h. GC-MS analysis identified 17 bioactive compounds, most with known antimicrobial properties. Molecular docking studies confirmed stable interactions between these compounds and key bacterial protein targets, supporting the observed bioactivity. As part of the genetic diversity, larval samples were identified by nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit I (COXI gene). These results were then compared with similar sequences that had already been put in GenBank. Moreover, a molecular docking study was performed to confirm and rationalize the observed biological activity. Overall, these results suggest that C. albiceps larval extract possesses antibacterial, antioxidant, and wound-healing activities and represents a safe, natural candidate for developing novel pharmaceutical and therapeutic agents.
期刊介绍:
Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports.
Research Areas Include:
-Pathogenesis
-Virulence factors
-Host susceptibility or resistance
-Immune mechanisms
-Identification, cloning and sequencing of relevant genes
-Genetic studies
-Viruses, prokaryotic organisms and protozoa
-Microbiota
-Systems biology related to infectious diseases
-Targets for vaccine design (pre-clinical studies)