Jannyson José Braz Jandú , Marliete Carvalho Costa , Elenildo Dário da Silva Júnior , Fernanda Miguel de Andrade , Marcia Vanusa da Silva , Luana Cassandra Breitenbach Barroso Coelho , Daniel Assis Santos , Ronald Silva Jesus , Karina Raquel Guilhon Machado , Maria das Graças Carneiro da Cunha , Luis Cláudio Nascimento Silva , Maria Tereza dos Santos Correia
{"title":"普克莫洛治疗可降低感染加蒂隐球菌免疫抑制小鼠的死亡率和真菌负担。","authors":"Jannyson José Braz Jandú , Marliete Carvalho Costa , Elenildo Dário da Silva Júnior , Fernanda Miguel de Andrade , Marcia Vanusa da Silva , Luana Cassandra Breitenbach Barroso Coelho , Daniel Assis Santos , Ronald Silva Jesus , Karina Raquel Guilhon Machado , Maria das Graças Carneiro da Cunha , Luis Cláudio Nascimento Silva , Maria Tereza dos Santos Correia","doi":"10.1016/j.micpath.2025.108043","DOIUrl":null,"url":null,"abstract":"<div><div>Cryptococcosis is an infectious disease caused mainly by two species <em>Cryptococcus neoformans</em> and <em>C. gattii</em>, and presents a myriad of symptoms and courses with pulmonary and central nervous system (CNS) clinical manifestations. The infection occurs mainly in immunosuppressed individuals, but <em>C. gattii</em> infections has garnered attention due to its prevalence among seemingly healthy individuals. The main treatment consolidated is lipid formulation amphotericin B plus 5-flucytosine, used on moderate-to-severe pulmonary and disseminated infection, followed by fluconazole. Fluconazole is used for mild-to-moderate pulmonary infection, but resistance phenomena have improved the focus on the search for new antimicrobials and immunoregulatory agents. The purpose of this work is analyze the use of pCramoll, an immunomodulatory lectin obtained from seeds of <em>Cratylia mollis</em> Mart [an endemic plant of Caatinga (Northeastern Brazil semi-arid area)], as therapy against the cryptococcosis in infected mice under iatrogenic immunosuppression by 200 mg/kg cyclophosphamide and 150 mg/kg of 5-fluorouracil association. We observed that the immunosuppression promotes susceptibility to <em>C. gattii</em> infection (Graphical Abstract), resulting in a reduction of 21 days median of mice survival, and an increase in the lung fungal burden at the seventh- and forty-day post-infection. With 1 μg/animal/day pCramoll treatment, the immunosuppressed mice exhibited less fungal burden on the lungs and brain, and were observed to have an increase of 100 % in their survival rate (Graphical Abstract). This work shows for the first time the use of plant lectins as possible immunoregulatory tools in immunosuppressed animals infected with <em>C. gattii</em>.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"209 ","pages":"Article 108043"},"PeriodicalIF":3.5000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"pCramoll therapy decreases mortality and fungal burden in immunosuppressed mice infected with Cryptococcus gattii\",\"authors\":\"Jannyson José Braz Jandú , Marliete Carvalho Costa , Elenildo Dário da Silva Júnior , Fernanda Miguel de Andrade , Marcia Vanusa da Silva , Luana Cassandra Breitenbach Barroso Coelho , Daniel Assis Santos , Ronald Silva Jesus , Karina Raquel Guilhon Machado , Maria das Graças Carneiro da Cunha , Luis Cláudio Nascimento Silva , Maria Tereza dos Santos Correia\",\"doi\":\"10.1016/j.micpath.2025.108043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Cryptococcosis is an infectious disease caused mainly by two species <em>Cryptococcus neoformans</em> and <em>C. gattii</em>, and presents a myriad of symptoms and courses with pulmonary and central nervous system (CNS) clinical manifestations. The infection occurs mainly in immunosuppressed individuals, but <em>C. gattii</em> infections has garnered attention due to its prevalence among seemingly healthy individuals. The main treatment consolidated is lipid formulation amphotericin B plus 5-flucytosine, used on moderate-to-severe pulmonary and disseminated infection, followed by fluconazole. Fluconazole is used for mild-to-moderate pulmonary infection, but resistance phenomena have improved the focus on the search for new antimicrobials and immunoregulatory agents. The purpose of this work is analyze the use of pCramoll, an immunomodulatory lectin obtained from seeds of <em>Cratylia mollis</em> Mart [an endemic plant of Caatinga (Northeastern Brazil semi-arid area)], as therapy against the cryptococcosis in infected mice under iatrogenic immunosuppression by 200 mg/kg cyclophosphamide and 150 mg/kg of 5-fluorouracil association. We observed that the immunosuppression promotes susceptibility to <em>C. gattii</em> infection (Graphical Abstract), resulting in a reduction of 21 days median of mice survival, and an increase in the lung fungal burden at the seventh- and forty-day post-infection. With 1 μg/animal/day pCramoll treatment, the immunosuppressed mice exhibited less fungal burden on the lungs and brain, and were observed to have an increase of 100 % in their survival rate (Graphical Abstract). This work shows for the first time the use of plant lectins as possible immunoregulatory tools in immunosuppressed animals infected with <em>C. gattii</em>.</div></div>\",\"PeriodicalId\":18599,\"journal\":{\"name\":\"Microbial pathogenesis\",\"volume\":\"209 \",\"pages\":\"Article 108043\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbial pathogenesis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0882401025007685\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial pathogenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0882401025007685","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
pCramoll therapy decreases mortality and fungal burden in immunosuppressed mice infected with Cryptococcus gattii
Cryptococcosis is an infectious disease caused mainly by two species Cryptococcus neoformans and C. gattii, and presents a myriad of symptoms and courses with pulmonary and central nervous system (CNS) clinical manifestations. The infection occurs mainly in immunosuppressed individuals, but C. gattii infections has garnered attention due to its prevalence among seemingly healthy individuals. The main treatment consolidated is lipid formulation amphotericin B plus 5-flucytosine, used on moderate-to-severe pulmonary and disseminated infection, followed by fluconazole. Fluconazole is used for mild-to-moderate pulmonary infection, but resistance phenomena have improved the focus on the search for new antimicrobials and immunoregulatory agents. The purpose of this work is analyze the use of pCramoll, an immunomodulatory lectin obtained from seeds of Cratylia mollis Mart [an endemic plant of Caatinga (Northeastern Brazil semi-arid area)], as therapy against the cryptococcosis in infected mice under iatrogenic immunosuppression by 200 mg/kg cyclophosphamide and 150 mg/kg of 5-fluorouracil association. We observed that the immunosuppression promotes susceptibility to C. gattii infection (Graphical Abstract), resulting in a reduction of 21 days median of mice survival, and an increase in the lung fungal burden at the seventh- and forty-day post-infection. With 1 μg/animal/day pCramoll treatment, the immunosuppressed mice exhibited less fungal burden on the lungs and brain, and were observed to have an increase of 100 % in their survival rate (Graphical Abstract). This work shows for the first time the use of plant lectins as possible immunoregulatory tools in immunosuppressed animals infected with C. gattii.
期刊介绍:
Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports.
Research Areas Include:
-Pathogenesis
-Virulence factors
-Host susceptibility or resistance
-Immune mechanisms
-Identification, cloning and sequencing of relevant genes
-Genetic studies
-Viruses, prokaryotic organisms and protozoa
-Microbiota
-Systems biology related to infectious diseases
-Targets for vaccine design (pre-clinical studies)