普克莫洛治疗可降低感染加蒂隐球菌免疫抑制小鼠的死亡率和真菌负担。

IF 3.5 3区 医学 Q3 IMMUNOLOGY
Jannyson José Braz Jandú , Marliete Carvalho Costa , Elenildo Dário da Silva Júnior , Fernanda Miguel de Andrade , Marcia Vanusa da Silva , Luana Cassandra Breitenbach Barroso Coelho , Daniel Assis Santos , Ronald Silva Jesus , Karina Raquel Guilhon Machado , Maria das Graças Carneiro da Cunha , Luis Cláudio Nascimento Silva , Maria Tereza dos Santos Correia
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引用次数: 0

摘要

隐球菌病是一种主要由新型隐球菌和C. gatti两种病原菌引起的传染病,具有多种症状和病程,临床表现为肺部和中枢神经系统。这种感染主要发生在免疫抑制的个体中,但由于在看似健康的个体中流行,加蒂杆菌感染引起了人们的注意。主要治疗方案是脂质制剂两性霉素B加5-氟胞嘧啶,用于中重度肺部感染和播散性感染,其次是氟康唑。氟康唑用于轻度至中度肺部感染,但耐药现象使人们更加关注寻找新的抗菌剂和免疫调节剂。本研究的目的是分析pCramoll(一种从Cratylia mollis Mart [Caatinga(巴西东北部半干旱区)的特有植物]种子中提取的免疫调节凝集素)对200 mg/Kg环磷酰胺和150 mg/Kg 5-氟尿嘧啶联合免疫抑制感染小鼠的隐球菌病的治疗作用。我们观察到免疫抑制促进了对C. gtii感染的易感性(图形摘要),导致小鼠存活的中位数减少21天,并且在感染后第7天和第40天肺部真菌负荷增加。在1 μg/只/天的pCramoll处理下,免疫抑制小鼠的肺和脑真菌负荷减轻,存活率提高100%(图摘要)。这项工作首次显示了植物凝集素作为免疫抑制动物感染C. gatti的可能的免疫调节工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

pCramoll therapy decreases mortality and fungal burden in immunosuppressed mice infected with Cryptococcus gattii

pCramoll therapy decreases mortality and fungal burden in immunosuppressed mice infected with Cryptococcus gattii
Cryptococcosis is an infectious disease caused mainly by two species Cryptococcus neoformans and C. gattii, and presents a myriad of symptoms and courses with pulmonary and central nervous system (CNS) clinical manifestations. The infection occurs mainly in immunosuppressed individuals, but C. gattii infections has garnered attention due to its prevalence among seemingly healthy individuals. The main treatment consolidated is lipid formulation amphotericin B plus 5-flucytosine, used on moderate-to-severe pulmonary and disseminated infection, followed by fluconazole. Fluconazole is used for mild-to-moderate pulmonary infection, but resistance phenomena have improved the focus on the search for new antimicrobials and immunoregulatory agents. The purpose of this work is analyze the use of pCramoll, an immunomodulatory lectin obtained from seeds of Cratylia mollis Mart [an endemic plant of Caatinga (Northeastern Brazil semi-arid area)], as therapy against the cryptococcosis in infected mice under iatrogenic immunosuppression by 200 mg/kg cyclophosphamide and 150 mg/kg of 5-fluorouracil association. We observed that the immunosuppression promotes susceptibility to C. gattii infection (Graphical Abstract), resulting in a reduction of 21 days median of mice survival, and an increase in the lung fungal burden at the seventh- and forty-day post-infection. With 1 μg/animal/day pCramoll treatment, the immunosuppressed mice exhibited less fungal burden on the lungs and brain, and were observed to have an increase of 100 % in their survival rate (Graphical Abstract). This work shows for the first time the use of plant lectins as possible immunoregulatory tools in immunosuppressed animals infected with C. gattii.
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来源期刊
Microbial pathogenesis
Microbial pathogenesis 医学-免疫学
CiteScore
7.40
自引率
2.60%
发文量
472
审稿时长
56 days
期刊介绍: Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)
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