IF 2.5 3区工程技术

Micron Pub Date : 2025-04-14 DOI: 10.1016/j.micron.2025.103824

Shin Inamoto, Akiyo Yoshida, Ayaka Yamagishi, Naoto Kaneko, Yuji Otsuka

引用次数: 0

Comparative morphology of damsel bugs legs’ microstructure (Insecta: Hemiptera: Nabidae) 豆虫腿微结构的比较形态学研究（昆虫亚目：半翅目：豆虫科）

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-10 DOI: 10.1016/j.micron.2025.103823

Adrian Masłowski , Artur Taszakowski , Jolanta Brożek

{"title":"Comparative morphology of damsel bugs legs’ microstructure (Insecta: Hemiptera: Nabidae)","authors":"Adrian Masłowski , Artur Taszakowski , Jolanta Brożek","doi":"10.1016/j.micron.2025.103823","DOIUrl":"10.1016/j.micron.2025.103823","url":null,"abstract":"<div><div>Nabidae, damsel bugs (Hemiptera: Heteroptera: Cimicomorpha) is a small family of predatory insects with poorly understood phylogenetic relationships. They are characterized by their raptorial fore- and to a lesser extend also midlegs with modified tibiae and femora covered with diverse types of armature consisting of highly sclerotized cuticular structures. The legs of nabids are also covered by various sensory organs and other structures used for cleaning the body, facilitate movement or with unknown functions. In this study, we describe the fine morphology of all these structures on the fore-, mid- and hindlegs of nine species from both subfamilies and three tribes of Nabidae (Gorpini, Nabini and Prostemmatini). The comparison of raptorial legs of different taxa is provided, as well as a discussion on mechanisms of holding the prey and the utility of their morphology in phylogenetic analysis. The grasping apparatuses of the studied species were classified into four morphological types based on the specific structures found in different taxa and development of the <em>fossula spongiosa</em>. The descriptions of other cuticular structures occurring on the legs are also presented. They are subdivided by their morphological features into numerous types in each species individually. In the discussion we compare these structures between the studied species and with other groups of Cimicomorpha. Some of them are common and were found in all species (and also in other insects) and some are specific for subfamily or genera. We also discuss the most probable function for each type of structure.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"195 ","pages":"Article 103823"},"PeriodicalIF":2.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and use of a flow cell for observing evolving solid-fluid interfaces in a scanning electron microscope 在扫描电子显微镜下设计和使用流动池来观察不断变化的固液界面

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-10 DOI: 10.1016/j.micron.2025.103825

R. Podor , J. Salacroup , H.P. Brau , J. Lautru , S. Szenknect , A. Candeias

{"title":"Design and use of a flow cell for observing evolving solid-fluid interfaces in a scanning electron microscope","authors":"R. Podor , J. Salacroup , H.P. Brau , J. Lautru , S. Szenknect , A. Candeias","doi":"10.1016/j.micron.2025.103825","DOIUrl":"10.1016/j.micron.2025.103825","url":null,"abstract":"<div><div>The development of a flow cell dedicated to the direct observation of the interaction between a fluid (the fluid being a gas or a liquid) and a solid in a scanning electron microscope is reported. This fluid flow cell has two main differences and advantages compared with existing devices. Firstly, it has been designed to allow direct observation of complex corrosion, dissolution, nucleation and/or growth processes taking place at solid materials surface. Secondly, the fluid circulates continuously in the cell maintaining constant chemical conditions thanks to the renewal of the fluid in contact with the solid. An electron-transparent SiN<sub>x</sub> window is used to isolate the interior of the flow cell from the vacuum of the SEM chamber. The surface of the sample is observed by recording images in backscattered electron mode. The contrasts observed in this mode are in good agreement with the results of Monte-Carlo simulations of electron trajectories and backscattered electron emissions carried out on model systems. Monte-Carlo simulations are used to determine the operating limits of the flow cell.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"195 ","pages":"Article 103825"},"PeriodicalIF":2.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposome dimensions at a glance by bright-field microscopy and fractal analysis 光场显微镜和分形分析的脂质体尺寸一目了然

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-05 DOI: 10.1016/j.micron.2025.103822

R. Pérez-Isidoro , A. Jessica Díaz-Salazar , S. Pérez-Casas , L.J. Bernal-Sánchez , E. Saldívar-Guerra , F.J. Guevara-Pantoja

{"title":"Liposome dimensions at a glance by bright-field microscopy and fractal analysis","authors":"R. Pérez-Isidoro , A. Jessica Díaz-Salazar , S. Pérez-Casas , L.J. Bernal-Sánchez , E. Saldívar-Guerra , F.J. Guevara-Pantoja","doi":"10.1016/j.micron.2025.103822","DOIUrl":"10.1016/j.micron.2025.103822","url":null,"abstract":"<div><div>Patterns from evaporated micro-droplets of complex fluids represent an emerging method for biological analysis. Although the driving forces behind the interactions in droplet evaporation of liquids have been vastly explored, an analytical technique derived from this knowledge needs to be well-established. In this work, we take advantage of the knowledge of the patterns found after the evaporation of microdroplets of lipid vesicles with potassium chloride to introduce a reliable method to differentiate the micro- and nano-size of liposomes composed of zwitterionic and anionic phospholipids. We use a statistical index of complexity, fractal dimension, to correlate the liposome size with the pattern index. We conclude that the values of fractal dimensions of 1.7 ( ± 0<em>.</em>1) that describe a monofractal can be related to the nanoscale range of unilamellar vesicles; otherwise, the vesicle sizes range the microscale. This suggests that our method could be a practical reference to reveal the nanoscale dimension of several systems in biophysics, bioengineering, and nanotechnology.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"195 ","pages":"Article 103822"},"PeriodicalIF":2.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Density-dependent colour scanning electron microscopy (DDC-SEM). Applications in the study of calcified tissues and visual impact 密度依赖性彩色扫描电子显微镜（DDC-SEM）。在钙化组织和视觉冲击研究中的应用

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-01 DOI: 10.1016/j.micron.2025.103821

Elena Tsolaki , Luke Hunter , Adrian H. Chester , Sergio Bertazzo

引用次数: 0

Localisation and structure of sclerenchyma fibres in the stem of Sida hermaphrodita (L.) Rusby 雌雄Sida茎厚壁组织纤维的定位与结构Rusby

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-01 DOI: 10.1016/j.micron.2025.103820

Aleksandra Seta-Koselska , Ewa Szczuka , Agata Leszczuk , Ilona Mazurkiewicz

{"title":"Localisation and structure of sclerenchyma fibres in the stem of Sida hermaphrodita (L.) Rusby","authors":"Aleksandra Seta-Koselska , Ewa Szczuka , Agata Leszczuk , Ilona Mazurkiewicz","doi":"10.1016/j.micron.2025.103820","DOIUrl":"10.1016/j.micron.2025.103820","url":null,"abstract":"<div><div>Contemporary consumers value more sustainable products, necessitating the sustainable use of plants, including fibre crops. Plant sclerenchyma fibres are a source of raw material used in various areas of human life. The present study was focused on analyses of sclerenchyma fibres in the Virginia mallow (<em>Sida hermaphrodita</em> (L.) Rusby). This energy plant, which is known for its many uses, was examined in terms of the location and differentiation of sclerenchyma fibres. They are present in the stem and are arranged in two rings formed by bundles with variable thickness. The structure of sclerenchyma fibres is typical of strengthening tissue. A characteristic trait of the studied species is the presence of various terminal fragments of dead cells in the sclerenchyma tissue. The surface of sclerenchyma fibres was examined using atomic force microscopy (AFM) for the first time. The analyses showed a varied surface sculpture of the fibre surface, depending on the method of preparation of the fibres before analyses. The study may constitute the basis for further investigations of the potential use of <em>S. hermaphrodita</em> sclerenchyma fibres, which may indicate a novel application of this plant. The potential use of a new source of sclerenchyma fibres obtained from the stem of <em>S</em>. <em>hermaphrodita</em> may support sustainable development.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"194 ","pages":"Article 103820"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two- and three-dimensional electron imaging of beam-sensitive specimens 光束敏感样品的二维和三维电子成像

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-01 DOI: 10.1016/j.micron.2025.103819

R.F. Egerton

引用次数: 0

A review of denoising methods in single-particle cryo-EM 单粒子低温电镜去噪方法综述

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-29 DOI: 10.1016/j.micron.2025.103817

Linhua Jiang , Bo Zhu , Wei Long , Jiahao Xu , Yi Wu , Yao-Wang Li

引用次数: 0

Dynamics of single Au nanoparticles on graphene simultaneously in real- and diffraction space by time-series convergent beam electron diffraction 用时间序列会聚束电子衍射研究石墨烯上单个金纳米粒子同时在实空间和衍射空间中的动力学

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-23 DOI: 10.1016/j.micron.2025.103814

Sara Mustafi , Rongsheng Cai , Sam Sullivan-Allsop , Matthew Smith , Nicholas J. Clark , Matthew Lindley , Ding Peng , Kostya S. Novoselov , Sarah J. Haigh , Tatiana Latychevskaia

{"title":"Dynamics of single Au nanoparticles on graphene simultaneously in real- and diffraction space by time-series convergent beam electron diffraction","authors":"Sara Mustafi , Rongsheng Cai , Sam Sullivan-Allsop , Matthew Smith , Nicholas J. Clark , Matthew Lindley , Ding Peng , Kostya S. Novoselov , Sarah J. Haigh , Tatiana Latychevskaia","doi":"10.1016/j.micron.2025.103814","DOIUrl":"10.1016/j.micron.2025.103814","url":null,"abstract":"<div><div>Convergent beam electron diffraction (CBED) on two-dimensional materials allows simultaneous recording of the real-space image (tens of nanometers in size) and diffraction pattern of the same sample in one single-shot intensity measurement. In this study, we employ time-series CBED to visualize single Au nanoparticles deposited on graphene. The real-space image of the probed region, with the amount, size, and positions of single Au nanoparticles, is directly observed in the zero-order CBED disk, while the atomic arrangement of the Au nanoparticles is available from the intensity distributions in the higher-order CBED disks. From the time-series CBED patterns, the movement of a single Au nanoparticle with rotation up to 4° was recorded. We also observed facet diffraction lines ̶ intense bright lines formed between the CBED disks of the Au nanoparticle, which we explain by diffraction at the Au nanoparticle's facets. This work showcases CBED as a useful technique for studying adsorbates on graphene using Au nanoparticles as a model platform, and paves the way for future studies of different objects deposited on graphene.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"194 ","pages":"Article 103814"},"PeriodicalIF":2.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopy-based methods for characterizing autophagy and understanding its dynamics in resin secretion 基于显微镜的自噬特征描述和树脂分泌动态了解方法

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-20 DOI: 10.1016/j.micron.2025.103818

Shelly F. de Carvalho , Elton L. Scudeler , Silvia R. Machado

{"title":"Microscopy-based methods for characterizing autophagy and understanding its dynamics in resin secretion","authors":"Shelly F. de Carvalho , Elton L. Scudeler , Silvia R. Machado","doi":"10.1016/j.micron.2025.103818","DOIUrl":"10.1016/j.micron.2025.103818","url":null,"abstract":"<div><div>Resin-secretory canals are a common feature of Anacardiaceae plants, and their resins have widespread applications in both industry and medicine. Cytological evidence strongly supports the occurrence of autophagy during the development of resin-secreting glands in several species of this family, including <em>Anacardium humile</em>. However, systematic investigations focusing on this process in these glands remain limited. This study aimed to enhance our understanding of autophagy in <em>A. humile</em> resin glands by elucidating its occurrence, timing, and specific mechanisms during the secretory cycle. Standard transmission electron microscopy techniques were used in conjunction with the cytochemical assays. Immunogold labeling and confocal immunofluorescence studies were conducted to identify autophagosomes and other autophagy-related structures. Two distinct types of autophagy have been identified, each associated with a specific phase of the secretory cycle. Macroautophagy predominates at the peak of secretion, whereas microautophagy occurs during the final stages of the cycle. As an integral component of the secretory process, autophagosomes degrade cytoplasmic components and organelles before fusing with the lysosomal vacuoles. In contrast to previous studies reporting extensive cellular degradation at the end of the resin-secretory cycle, often interpreted as a form of programmed cell death, no evidence of mega-autophagy was observed in this study. These findings suggest that the precise regulation of autophagy timing and intensity is crucial for maintaining the functional integrity of resin-secreting cells. Furthermore, the potential interplay between autophagic activity and terpene biosynthesis requires further investigation in the context of resin-secretory canal physiology.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103818"},"PeriodicalIF":2.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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