IF 2.5 3区工程技术

Micron Pub Date : 2025-05-26 DOI: 10.1016/j.micron.2025.103860

Nobuko Ohmido , Channarong Sartsanga , Astari Dwiranti

{"title":"Structure and compaction of plant chromosomes: Studies using advanced electron microscopy","authors":"Nobuko Ohmido , Channarong Sartsanga , Astari Dwiranti","doi":"10.1016/j.micron.2025.103860","DOIUrl":"10.1016/j.micron.2025.103860","url":null,"abstract":"<div><div>Chromosomes serve as fundamental units for the transmission of genetic information, and understanding their higher-order structure has been a longstanding research challenge. This review highlights recent advances in plant chromosome structure analysis, focusing on electron microscopy techniques that enable nanoscale visualization of chromatin architecture. Improvements in chromosome preparation, including chromosome isolation and ionic liquid coating, have enhanced the preservation of chromosome structure. Scanning Electron Microscopy (SEM), Focused Ion Beam SEM (FIB-SEM) and High-Voltage Transmission Electron Microscopy (HVTEM) have revealed detailed features of chromatin folding in centromeric and non-centromeric regions. Proteomic studies have identified key chromosomal proteins, such as topoisomerase II and nucleolar proteins, contributing to chromosome condensation and stability. The role of divalent cations and RNA in chromatin compaction are also discussed. Integrating these findings, this review provides an overview of technological advancements and their impact on elucidating chromosome architecture.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"196 ","pages":"Article 103860"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the relationship between oxygen K edge fine structure and cobalt/nickel content in magnetite-based core-shell nanoparticles 磁铁矿基核壳纳米颗粒中氧K边缘精细结构与钴/镍含量的关系

IF 2.5 3区工程技术

Micron Pub Date : 2025-05-22 DOI: 10.1016/j.micron.2025.103858

Simon Hettler , Raul Arenal

引用次数: 0

Electron irradiation-based cleaning of the scanning electron microscope and its samples 扫描电镜及其样品的电子辐照清洗

IF 2.5 3区工程技术

Micron Pub Date : 2025-05-20 DOI: 10.1016/j.micron.2025.103857

András E. Vladár

{"title":"Electron irradiation-based cleaning of the scanning electron microscope and its samples","authors":"András E. Vladár","doi":"10.1016/j.micron.2025.103857","DOIUrl":"10.1016/j.micron.2025.103857","url":null,"abstract":"<div><div>The deposition of carbonaceous material under electron beam irradiation is an old and persistent problem in scanning electron microscopy. It impedes high-resolution imaging and measurements, especially at the nanometer scale. The emergence of contamination is a complex process of adsorption, dissociation, and desorption of carbonaceous molecules. Depending on the kind and amount of precursor molecules, vacuum, sample material, temperature, and the intensity and energy of the irradiating electrons, deposition can overwhelm removal or the other way. Fortunately, with the introduction and commercial availability of low-energy, plasma-based, and other cleaning devices, contamination can be reduced to non-detectable levels. Plasma devices, working in low vacuum, which is necessary to start and sustain plasma generation, can effectively remove contamination precursor hydrocarbon molecules to the extent that a clean sample can be imaged and continuously measured for hours without deposition of any perceptible amount of carbonaceous contamination. Here, we report on the results of a new and effective cleaning device that has recently become available, which is different from plasma cleaning devices. It is based on low-energy electron irradiation and works in high and ultra-high vacuum (UHV).</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"196 ","pages":"Article 103857"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between hollow cone illumination and wavelet transform methods for chromatin fiber orientation measurement 空心锥照明与小波变换在染色质纤维取向测量中的比较

IF 2.5 3区工程技术

Micron Pub Date : 2025-05-20 DOI: 10.1016/j.micron.2025.103859

Misa Hayashida , Rinyaporn Phengchat , Marek Malac , Keiko Shimada , Shigeo Mori , Yoshio Takahashi , Ken Harada

{"title":"A comparison between hollow cone illumination and wavelet transform methods for chromatin fiber orientation measurement","authors":"Misa Hayashida , Rinyaporn Phengchat , Marek Malac , Keiko Shimada , Shigeo Mori , Yoshio Takahashi , Ken Harada","doi":"10.1016/j.micron.2025.103859","DOIUrl":"10.1016/j.micron.2025.103859","url":null,"abstract":"<div><div>Nucleosomes are proven to be the fundamental unit of chromosome structure. The stacking and folding of the nucleosome fibers within a chromosome is not fully understood. One of the reasons for the incomplete understanding of chromosome internal structure is that a nucleosome, about 11 nm in diameter, can not be resolved within the large chromatids (∼ 700 nm diameter) of a chromosome. In a transmission electron microscope (TEM), the large difference in size between the small diameter nucleosomes and a chromosome results in an extremely low contrast arising from individual nucleosomes. Consequently, the nucleosome fiber can not be detected within an intact chromosome. In this study, we compared two different methods in TEM, namely the hollow cone illumination (HCI) TEM and wavelet transform (WT) analysis on bright-field TEM (BFTEM) images, to analyze internal structure of chromosomes at length scales ranging from 10 to 30 nm. Isolated chromosomes were expanded and the orientation of the chromatin fibers was measured by HCI TEM and by WT applied to BFTEM. We demonstrated that the results obtained by the two methods are in an agreement.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"196 ","pages":"Article 103859"},"PeriodicalIF":2.5,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image drift compensation in scanning electron microscopy facilitated by an external scanning and imaging system 扫描电子显微镜中的图像漂移补偿是由外部扫描成像系统实现的

IF 2.5 3区工程技术

Micron Pub Date : 2025-05-17 DOI: 10.1016/j.micron.2025.103848

Ling‘en Liu , Yixu Zhang , Ni Wang , Liang Tang , Chaoyang Yan , Jianli Zhou , Wenjie Gao , Yuefei Zhang , Junxia Lv , Ze Zhang

{"title":"Image drift compensation in scanning electron microscopy facilitated by an external scanning and imaging system","authors":"Ling‘en Liu , Yixu Zhang , Ni Wang , Liang Tang , Chaoyang Yan , Jianli Zhou , Wenjie Gao , Yuefei Zhang , Junxia Lv , Ze Zhang","doi":"10.1016/j.micron.2025.103848","DOIUrl":"10.1016/j.micron.2025.103848","url":null,"abstract":"<div><div>Microstructures significantly influence the physical properties of materials. Characterizing the evolution of materials' microstructures is helpful for exploring the processing techniques and understanding the thermodynamic properties of materials. However, the in-situ experiments based on the scanning electron microscope (SEM) often suffer from non-uniform image drift distortion, which severely interferes with the imaging and characterization. Therefore, in this study, we develop an external scanning and imaging system for dynamic image drift compensation during the in-situ SEM experiments. The drifted image was dynamically corrected to the center of view by changing the path of the electron beams. The proposed method was compared with three conventional image correction methods to validate its effectiveness in two scenarios, i.e., in-situ translation experiment and in-situ heating experiment. The results showed that the image registration technique combined with the electron beam trajectory correction effectively compensated the image drift caused by irregular sample motion. Compared with existing image post-processing methods, we have achieved real-time drift compensation of the images. For the secondary electron (SE) image with a resolution of 1024 × 1024 pixels compensated based on the method proposed in this paper, the maximum pixel loss within the field of view is only 3 pixels. This technology can effectively correct image drift caused by high temperatures during the in-situ progress, thereby helping material characterization.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"196 ","pages":"Article 103848"},"PeriodicalIF":2.5,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive AFM image reconstruction through frequency coefficient selection based on block compressed sensing 基于块压缩感知的频率系数选择自适应AFM图像重建

IF 2.5 3区工程技术

Micron Pub Date : 2025-05-16 DOI: 10.1016/j.micron.2025.103849

Yifan Hu, Yingzi Li, Peng Cheng, Rui Lin, Jianqiang Qian, Quan Yuan, Yanan Chen

{"title":"Adaptive AFM image reconstruction through frequency coefficient selection based on block compressed sensing","authors":"Yifan Hu, Yingzi Li, Peng Cheng, Rui Lin, Jianqiang Qian, Quan Yuan, Yanan Chen","doi":"10.1016/j.micron.2025.103849","DOIUrl":"10.1016/j.micron.2025.103849","url":null,"abstract":"<div><div>Atomic force microscope (AFM) is a valuable instrument for nano-scale imaging. Traditional AFM demands long time to obtain AFM images due to point-by-point imaging. AFM based on compressed sensing (CS-AFM) is able to obtain AFM images by reconstructing from incomplete measurement. Block compressed sensing (BCS) further shortens the reconstruction time but focuses on the topography of samples, which loses necessary frequency information. Different subblocks exhibit various characteristics by sparse transformation. In this paper an adaptive frequency coefficient selection method based on BCS is proposed to enhance reconstruction quality. We focus on the frequency domain of the AFM image, applying sparse transformation to obtain the corresponding frequency coefficients. We then select partial coefficients from all frequency components as the feature information for each subblock during reconstruction, followed by an inverse sparse transformation to obtain the final reconstructed image. The proposed method outperforms both iterative selection-based BCS and ordinary BCS, achieving the highest PSNR and SSIM values across various sampling ratio while maintaining comparable reconstruction times, which verifies its ability to fast reconstruct AFM images with high quality.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"196 ","pages":"Article 103849"},"PeriodicalIF":2.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-resolution live imaging of tardigrade response to anoxia

IF 2.5 3区工程技术

Micron Pub Date : 2025-05-09 DOI: 10.1016/j.micron.2025.103847

Anna-Mari Haapanen-Saaristo , Sara Calhim , Ilkka Paatero

引用次数: 0

Microscopic study of local thermal, electrical and structural properties of ZnMgO thin films on different substrates 不同衬底上ZnMgO薄膜的局部热、电学和结构性能的微观研究

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-19 DOI: 10.1016/j.micron.2025.103837

Anna Kazmierczak-Balata , Justyna Juszczyk , Dominika Trefon-Radziejewska , Adrian Cernescu , Roman Minikayev , Mieczyslaw A. Pietrzyk

{"title":"Microscopic study of local thermal, electrical and structural properties of ZnMgO thin films on different substrates","authors":"Anna Kazmierczak-Balata , Justyna Juszczyk , Dominika Trefon-Radziejewska , Adrian Cernescu , Roman Minikayev , Mieczyslaw A. Pietrzyk","doi":"10.1016/j.micron.2025.103837","DOIUrl":"10.1016/j.micron.2025.103837","url":null,"abstract":"<div><div>In this work we combine a group of scanning microscopy methods to investigate correlations between structural, thermal and electrical properties of ZnMgO thin films, influenced by the substrate material. The ZnMgO layers, grown by means of molecular beam epitaxy, were deposited on r- and m-plane Al<sub>2</sub>O<sub>3</sub> and Si (111) substrates. The X-ray diffraction measurements were introduced to verify crystallinity of investigated ZnMgO layers. We demonstrate the possibilities offered by combining selected scanning microscopy methods to gain deeper and more comprehensive insight into the interrelationship of structural and thermo-electrical properties of thin ZnMgO layers. Using atomic force microscopy, we analyzed the morphology, basic surface parameters and surface grains. Additionally, the Kelvin probe force microscopy allowed us to analyze the surface electron properties, such as the local contact potential difference and work function distribution. We used scanning thermal microscopy to determine local thermal conductivity values. Finally, the scattering-type scanning near-field optical microscopy provided us with information about the local free-carrier concentration. While maintaining a spatial resolution above 100 nm, proposed approach provides valuable information on the possibilities of using the studied layers in various nanosystems and devices for which the surface structure and thermo-electric properties play a key role.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"195 ","pages":"Article 103837"},"PeriodicalIF":2.5,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of a bacterial collagen after hydroxyproline incorporation 羟基脯氨酸结合后细菌胶原蛋白的结合

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-18 DOI: 10.1016/j.micron.2025.103832

Jacinta F. White, Yong Y. Peng, Xuen Ng , Denis Bartoschek , Veronica Glattauer, John A.M. Ramshaw

{"title":"Association of a bacterial collagen after hydroxyproline incorporation","authors":"Jacinta F. White, Yong Y. Peng, Xuen Ng , Denis Bartoschek , Veronica Glattauer, John A.M. Ramshaw","doi":"10.1016/j.micron.2025.103832","DOIUrl":"10.1016/j.micron.2025.103832","url":null,"abstract":"<div><div>Various bacterial collagen-like proteins have been previously described and shown to have a triple helical, (Gly-Xaa Yaa)<sub>n</sub> repeating structure. They are stable without needing any secondary modification of proline residues in the Yaa position to hydroxyproline, a characteristic feature of animal collagens. Hydroxyproline can, however, be introduced into recombinant bacterial collagen by co-translational incorporation during fermentation. However, this does not lead to full incorporation and introduces the hydroxyproline into both the Xaa and Yaa positions. It was suggested that the poor solubility of bacterial collagen samples with higher levels of incorporation of hydroxyproline could be due to an increase in protein association at neutral pH. In the present study, cryo-transmission electron microscopy was used to examine the nature and extent of any associations arising from hydroxyproline incorporation. This was examined further, using 2 smaller fragments, where the proline sites are predominantly in either the Xaa position or Yaa position. The present data confirm the importance of the presence of hydroxyproline in assisting in the association between collagen molecules.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"195 ","pages":"Article 103832"},"PeriodicalIF":2.5,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional digital elevation models reconstructed from stereoscopic image of platinum replica in sheared open osteoclasts 利用剪开破骨细胞中铂复制品的立体图像重建三维数字高程模型

IF 2.5 3区工程技术

Micron Pub Date : 2025-04-18 DOI: 10.1016/j.micron.2025.103834

Toshitaka Akisaka

{"title":"Three-dimensional digital elevation models reconstructed from stereoscopic image of platinum replica in sheared open osteoclasts","authors":"Toshitaka Akisaka","doi":"10.1016/j.micron.2025.103834","DOIUrl":"10.1016/j.micron.2025.103834","url":null,"abstract":"<div><div>Computer-generated microscopic images can be valuable tools for analyzing cell structure. We have used a computerized surface topography technique to convert platinum replica images into measurable 3D digital elevation model reconstructiondata. The commercially available Alicona MeX software can be successfully applied to the 3D reconstruction images of the platinum replicas, resulting in a series of digital elevation models in grayscale and coloured elevation maps in RGB mode of the selected area of interest. Here, we present accessible methods to analyze cell structures in sheared-open osteoclasts in 3D and at nanometre resolution, focusing on the podosome cytoskeleton, membrane-bound clathrin lattices, and surface topography. These structures on the surface of the ventral membrane appear to be highly characterized for their specific cellular functions. Extraction data from these reconstructed digital elevation models lead to the presentation of 3D information on some ultrastructural architectures on the ventral membrane, including the height of podosomes, the thickness of clathrin-coated structures and the non-coplanar surface of the flat clathrin lattices. In particular, we found that flat clathrin lattices appear on the curved surface of the basal part of the cell protrusions, or the non-coplanarity of their surface topography further indicates their morphological diversity. This new analytical approach provided a fast and easy way to reveal the ventral membrane surface structures in sheared open osteoclasts using high quality 3D reconstructed images.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"195 ","pages":"Article 103834"},"PeriodicalIF":2.5,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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