缓步动物对缺氧反应的高分辨率实时成像

IF 2.2 3区工程技术 Q1 MICROSCOPY

Micron Pub Date : 2025-05-09 DOI:10.1016/j.micron.2025.103847

Anna-Mari Haapanen-Saaristo , Sara Calhim , Ilkka Paatero

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引用次数: 0

摘要

众所周知，缓步动物能够忍受极端的环境条件，如高温、干旱和缺氧。对胁迫的分子响应已经有了详细的研究，但对隐生过程中的生理和形态发生变化了解较少。我们开发了新的活体高分辨率荧光显微镜方案，以可视化缓步动物对缺氧的反应。高分辨率延时成像使分析细胞形态和跟踪细胞运动在缺氧期间。这些分析揭示了储存细胞在形态、组成和运动方面的显著变化。我们的观察和新的成像方法可以用于研究缓步动物对应激的形态学和细胞反应。

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High-resolution live imaging of tardigrade response to anoxia

Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen – anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.

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来源期刊

Micron 工程技术-显微镜技术

CiteScore

4.30

自引率

4.20%

发文量

100

审稿时长

31 days

期刊介绍： Micron is an interdisciplinary forum for all work that involves new applications of microscopy or where advanced microscopy plays a central role. The journal will publish on the design, methods, application, practice or theory of microscopy and microanalysis, including reports on optical, electron-beam, X-ray microtomography, and scanning-probe systems. It also aims at the regular publication of review papers, short communications, as well as thematic issues on contemporary developments in microscopy and microanalysis. The journal embraces original research in which microscopy has contributed significantly to knowledge in biology, life science, nanoscience and nanotechnology, materials science and engineering.