Micron最新文献

{"title":"A simple, static and stage mounted direct electron detector based electron backscatter diffraction system","authors":"Tianbi Zhang ,&nbsp;T. Ben Britton","doi":"10.1016/j.micron.2023.103582","DOIUrl":"10.1016/j.micron.2023.103582","url":null,"abstract":"<div><p><span><span>To engineer the next generation of advanced materials<span> we must understand their microstructure, and this requires microstructural characterization. This can be achieved through the collection of high contrast, data rich, and insightful microstructural maps. Electron backscatter diffraction (EBSD) has emerged as a popular tool available within the scanning </span></span>electron microscope<span> (SEM), where maps are realized through the repeat capture and analysis of Kikuchi diffraction patterns. Typical commercial EBSD systems require large and sophisticated detectors that are mounted on the side of the SEM vacuum chamber which can be limiting in terms of widespread access to the technique. In this work, we present an alternative open-hardware solution based upon a compact EBSD system with a simple, </span></span>static<span> geometry that uses an off-the-shelf direct electron detector co-mounted with a sample. This simple stage is easy to manufacture and improves our knowledge of the diffraction geometry significantly. Microscope and detector control is achieved through software application programming interface (API) integration. After pattern capture, analysis of the diffraction patterns is performed using open-source analysis within AstroEBSD. To demonstrate the potential of this set up, we present two simple EBSD experiments using a line scan and area mapping. We hope that the present system can inspire simpler EBSD system design for widespread access to the EBSD technique and promote the use of open-source software and hardware in the workflow of EBSD experiments.</span></p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139036209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid scanning method for SICM based on autoencoder network","authors":"Wenlin Wu ,&nbsp;Xiaobo Liao ,&nbsp;Lei Wang ,&nbsp;Siyu Chen ,&nbsp;Jian Zhuang ,&nbsp;Qiangqiang Zheng","doi":"10.1016/j.micron.2023.103579","DOIUrl":"10.1016/j.micron.2023.103579","url":null,"abstract":"<div><p>Scanning Ion Conductance Microscopy (SICM) enables non-destructive imaging of living cells, which makes it highly valuable in life sciences, medicine, pharmacology, and many other fields. However, because of the uncertainty retrace height of SICM hopping mode, the time resolution of SICM is relatively low, which makes the device fail to meet the demands of dynamic scanning. To address above issues, we propose a fast-scanning method for SICM based on an autoencoder network. Firstly, we cut under-sampled images into small image lists. Secondly, we feed them into a self-constructed primitive-autoencoder super-resolution network to compute high-resolution images. Finally, the inferred scanning path is determined using the computed images to reconstruct the real high-resolution scanning path. The results demonstrate that the proposed network can reconstruct higher-resolution images in various super-resolution tasks of low-resolution scanned images. Compared to existing traditional interpolation methods, the average peak signal-to-noise ratio improvement is greater than 7.5823 dB, and the average structural similarity index improvement is greater than 0.2372. At the same time, using the proposed method for high-resolution image scanning leads to a 156.25% speed improvement compared to traditional methods. It opens up possibilities for achieving high-time resolution imaging of dynamic samples in SICM and further promotes the widespread application of SICM in the future.</p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138991249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized procedure for conventional TEM sample preparation using birefringence","authors":"Aleksander Brozyniak,&nbsp;Karin Stadlmann,&nbsp;Philipp Kürnsteiner,&nbsp;Heiko Groiss","doi":"10.1016/j.micron.2023.103580","DOIUrl":"10.1016/j.micron.2023.103580","url":null,"abstract":"<div><p>Specimens for quality transmission electron microscopy (TEM) analyses must fulfil a range of requirements, which demand high precision during the prior preparation process. In this work, an optimized procedure for conventional TEM specimen preparation is presented that exploits the thickness-dependence of interference colors occurring in birefringent materials. It facilitates the correct estimation of specimen thickness to avoid damage or breaking during mechanical thinning and reduces ion-milling times below 30 min. The benefits of the approach are shown on sapphire and silicon carbide cross-section samples. The presented method is equally suitable for assessing specimen thickness during dimpling and wedge-polishing, and is particularly useful at thicknesses below 20 µm, where the accuracy of mechanical techniques is insufficient. It is precise enough to be employed for a visual thickness estimation during the thinning process, but can be additionally optimized by analyzing the RGB spectrum of the occurring interference colors.</p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0968432823001786/pdfft?md5=19fe2318ca594c4e1282ebdbfc6603bf&pid=1-s2.0-S0968432823001786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138685385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voxel dose-limited resolution for thick beam-sensitive specimens imaged in a TEM or STEM","authors":"R.F. Egerton","doi":"10.1016/j.micron.2023.103576","DOIUrl":"10.1016/j.micron.2023.103576","url":null,"abstract":"<div><p>The resolution limit imposed by radiation damage is quantified in terms of a voxel dose-limited resolution (DLR), applicable to small features within a thick specimen. An analytical formula for this DLR is derived and applied to bright-field mass-thickness contrast from organic (polymer or biological) specimens of thickness between 400 nm and 20 µm. For a permissible dose of 330 MGy (typical of frozen-hydrated tissue), the TEM or STEM image resolution is determined by radiation damage rather than by lens aberrations or beam-broadening effects, which can be restricted by use of a small angle-limiting aperture. DLR is improved by a up to factor of 2 by increasing the primary-electron energy from 300 keV to 3 MeV, or by up to a factor of 3 by heavy-metal staining. For stained samples, a higher electron fluence allows better resolution but the improvement is modest because the voxel DLR is proportional to the 1/4 power of electron dose. The relevance of voxel and columnar DLR is discussed, for both thick and thin samples.</p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138685297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AB-PLS-DA: Pansharpening tailored for scanning electron microscopy and energy-dispersive X-ray spectrometry multimodal fusion","authors":"Tuomas Sihvonen ,&nbsp;Zina-Sabrina Duma ,&nbsp;Satu-Pia Reinikainen","doi":"10.1016/j.micron.2023.103578","DOIUrl":"10.1016/j.micron.2023.103578","url":null,"abstract":"<div><p>Pansharpening constitutes a category of data fusion techniques designed to enhance the spatial resolution of multispectral (MS) images by integrating spatial details from a high-resolution panchromatic (PAN) image. This process combines the high-spectral data of MS images with the rich spatial information of the PAN image, resulting in a pansharpened output ideal for more effective image analysis, such as object detection and environmental monitoring. Traditionally developed for satellite data, our paper introduces a novel pansharpening approach customized for the fusion of Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectrometry (EDS) data. The proposed method, grounded in Partial Least Squares regression with Discriminant Analysis (PLS-DA), significantly boosts the spatial resolution of EDS data while preserving spectral details. A key feature of this approach involves partitioning the PAN image into intensity bins and dynamically adapting this division in cases of overlapping compounds with similar average atomic numbers. We evaluate the method’s effectiveness using in-house EDS images obtained from both even and uneven sample surfaces. Comparative analysis against existing benchmarks and state-of-the-art pansharpening techniques demonstrates superior performance in both spectral and spatial quality indicators for our method.</p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0968432823001762/pdfft?md5=e76a68ed81bcb1478224412a46be1ae0&pid=1-s2.0-S0968432823001762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138685293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of resveratrol on SH-SY5Y cells studied by atomic force microscopy","authors":"Jiayao Yuan ,&nbsp;Zuobin Wang ,&nbsp;Jianjun Dong ,&nbsp;Mingyan Gao ,&nbsp;Fan Yang ,&nbsp;Hao Sun","doi":"10.1016/j.micron.2023.103577","DOIUrl":"10.1016/j.micron.2023.103577","url":null,"abstract":"<div><p>In this paper, the effects of resveratrol<span><span><span> on the viability, morphology, biomechanics and </span>bioelectricity<span> of SH-SY5Y cells were studied by atomic force microscopy<span>. MTT assay showed that resveratrol had a dose effect on SH-SY5Y cells, and its activity was related to drug concentration and drug action time. With the increase of resveratrol concentration or the extension of action time, the activity of SH-SY5Y cells decreased obviously. Atomic force microscope (AFM) was employed to quantitatively analyze the physical changes of cells. AFM study shows that resveratrol can transform SH-SY5Y cells from spindle to sphere, and increase the cell height and decrease the </span></span></span>cell adhesion<span>. Also, the elastic modulus increases under the action of low concentration of resveratrol decreases under the action of high concentration of resveratrol, and the electric signal decreases. This study reveals the impact of resveratrol on SH-SY5Y cells from the biological and biophysical perspectives, which is helpful for a more comprehensive understanding of the interaction mechanism between resveratrol and SH-SY5Y cells. These techniques have potential applications in evaluating the effects of chemical substances on cells and screening targeted drugs.</span></span></p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138581551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface morphology and microstructure of Bauhinia variegata L. flowers and leaves","authors":"Yan Xu","doi":"10.1016/j.micron.2023.103575","DOIUrl":"https://doi.org/10.1016/j.micron.2023.103575","url":null,"abstract":"<div><p><span>The main purpose of this research is to characterize the wettability and microstructure of </span><span><em>Bauhinia variegata</em></span><span><span><span> L. flowers and leaves, aiming to provide a biological template for the preparation of hydrophobic surfaces. The contact angle test results show that the surfaces of flowers and leaves are hydrophobic, and the contact angle of the adaxial side and abaxial side of the leaves is highly significant difference, which is mainly affected by the topological morphology of the surface microstructure. The topological structure of flower and leaf </span>surface morphology was revealed by scanning electron microscopy (SEM) and super-depth of field three-dimensional microscope. The results showed that the cell structure of flower surface array was the main factor leading to the </span>hydrophobicity<span> of flowers. The snowflake-like structure on the leaf surface is the main factor leading to hydrophobicity of leaves, and the burr structure of the micro-nano dual-level structure unique to the abaxial side of the leaf further enhances the hydrophobicity of the abaxial side, and the contact angle increases, resulting in a highly significant difference in wettability between the adaxial side and abaxial side. This provides an important biological template and reference value for the preparation of biomimetic materials.</span></span></p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138577807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micrometer insights into Nepeta genus: Pollen micromorphology unveiled","authors":"Shaista Jabeen ,&nbsp;Muhammad Zafar ,&nbsp;Mushtaq Ahmad ,&nbsp;M. Ajmal Ali ,&nbsp;Mohamed S. Elshikh ,&nbsp;Trobjon Makhkamov ,&nbsp;Oybek Mamarakhimov ,&nbsp;Akramjon Yuldashev ,&nbsp;Khislat Khaydarov ,&nbsp;Yusufjon Gafforov ,&nbsp;Babir Baysunov ,&nbsp;Afat O. Mammadova ,&nbsp;Laziza Botirova ,&nbsp;Shazia Sultana ,&nbsp;Salman Majeed ,&nbsp;Rozina ,&nbsp;Shabir Ahmad ,&nbsp;Aqsa Abid ,&nbsp;Abdurashid Rahmatov","doi":"10.1016/j.micron.2023.103574","DOIUrl":"10.1016/j.micron.2023.103574","url":null,"abstract":"<div><p><span>This study provides a comprehensive pollen micromorphology within the </span><span><em>Nepeta</em></span> genus, revealing intricate details about the pollen grains' structure and characteristics. The findings shed light on the evolutionary and taxonomical aspects of this plant genus, offering valuable insights for botanists and researchers studying <em>Nepeta</em><span> species. The pollen grains of 18 Nepeta species were studied using scanning electron microscopy (SEM) and light microscopy (LM) in Northern Pakistan. At the microscale, pollen quantitative measurements, qualitative traits, and diverse sculpturing patterns were reported and compared. Significant differences in pollen size, shape, ornamentation, and sculpturing patterns were discovered among the Nepeta species. Our data show that exine sculpturing is quite diverse, with most species exhibiting a reticulate perforate pollen pattern. </span><em>Nepeta connata, Nepeta discolor, Nepeta elliptica,</em><span> revealed a distinct bireticulate perforate exine stratification. Hexazonocolpate pollen is the most common. Furthermore, the surface membrane attributes of the colpus varied greatly, ranging from rough, scabrate, psilate, to sinuate patterns. Principal Component Analysis (PCA) was used to discover the key factors influencing pollen diversity. PCA results showed that polar and equatorial diameters, colpi size, and exine thickness were the most influential pollen features between </span><em>Nepeta</em> species. This study adds to our understanding of pollen morphology in the <em>Nepeta</em> genus, offering information on the vast range of characteristics found in this economically important group. The extensive characterization of pollen features provides useful insights for the categorization and differentiation of <em>Nepeta</em><span> species, adding to the Lamiaceae micromorphology.</span></p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138495151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of curcumin on malignant hepatocytes and mitochondria studied using atomic force microscopy","authors":"Shengli Zhang ,&nbsp;Ying Wang ,&nbsp;Bowei Wang ,&nbsp;Yi Zeng ,&nbsp;Jiani Li ,&nbsp;Xingyue Wang ,&nbsp;Cuihua Hu ,&nbsp;Zhankun Weng ,&nbsp;Zuobin Wang","doi":"10.1016/j.micron.2023.103573","DOIUrl":"https://doi.org/10.1016/j.micron.2023.103573","url":null,"abstract":"<div><p>Mitochondria<span><span> are emerging as potential targets for the cancer treatment. In this study, the effects of curcumin on the activity, migration, and mitochondrial membrane potential<span> (MMP) of malignant hepatocytes (SMMC-7721 cells) were determined using cell viability, migration, and MMP assays. Changes in the morphology and </span></span>biomechanics<span><span> of SMMC-7721 cells and their mitochondria were studied using both optical microscopy and atomic force microscopy (AFM). The cell survival rate, migration and MMP depended on the concentration of curcumin. Optical microscopy studies showed that curcumin altered the cell morphology. AFM studies showed that the changes in the morphology and </span>nanomechanics<span> of SMMC-7721 cells and their mitochondria, were induced by curcumin. As the concentration of curcumin increased, the cell length, width, and adhesion decreased, but the height, roughness and Young's modulus increased. In contrast, the mitochondrial length, width, height and roughness increased, but the adhesion and Young's modulus decreased. There was a close relationship between mitochondria and cells in terms of function, morphology and biomechanics. This study shows the effects of curcumin on SMMC-7721 cells and their mitochondria from biology and biophysics perspectives. The findings aid in comprehensively understanding the interactions between mitochondria and malignant hepatocytes.</span></span></span></p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138474853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exit wave function reconstruction from two defocus images using neural network","authors":"Ziyi Meng ,&nbsp;Wenquan Ming ,&nbsp;Yutao He ,&nbsp;Ruohan Shen ,&nbsp;Jianghua Chen","doi":"10.1016/j.micron.2023.103564","DOIUrl":"10.1016/j.micron.2023.103564","url":null,"abstract":"<div><p>Wave function reconstruction from one or two defocus images is promising for live atomic resolution imaging in transmission electron microscopy. However, a robust and accurate reconstruction method we still need more attention. Here, we present a neural-network-based wave function reconstruction method, EWR-NN, that enables accurate wave function reconstruction from only two defocus images. Results from both simulated and two different experimental defocus series show that the EWR-NN method has better performance than the widely-used iterative wave function reconstruction (IWFR) method. Influence of image number, defocus deviation, residual image shifts and noise level were considered to validate the performance of EWR-NN under practical conditions. It is seen that these factors will not influence the arrangement of atom columns in the reconstructed phase images, while they can alter the absolute values of all-atom columns and degrade the contrast of the phase images.</p></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135614186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}