IF 2.5 3区工程技术

Micron Pub Date : 2025-03-16 DOI: 10.1016/j.micron.2025.103812

Zeynep Büşra Erarslan , Şükran Kültür

{"title":"A study to elucidate the taxonomy of the genus Doronicum based on morphological and anatomical studies","authors":"Zeynep Büşra Erarslan , Şükran Kültür","doi":"10.1016/j.micron.2025.103812","DOIUrl":"10.1016/j.micron.2025.103812","url":null,"abstract":"<div><div>The main purpose of this study is the morphological and anatomical characterization of <em>Doronicum</em> L. taxa naturally grown in Türkiye. In this context, morphology and anatomy of 11 species, and 1 subspecies of <em>Doronicum</em> was researched in detail by stereo and light microscopy. Moreover, hierarchical cluster analysis (HCA) and principal component analysis (PCA) were performed to identify closely related species and the potential anatomical characters which could be used to delimitation the studied taxa. Although the anatomical features of the examined taxa are generally similar to each other, some differences have been identified. Since indumentum is significant in distinguishing taxa, trichome types and dimensions were also evaluated. The first four PCs explained about 66.28 % of total variability. Some anatomical characters such as cortical cell (stem), collenchyma thickness (stem), adaxial epidermis cell length (leaf), spongy parenchyma thickness (leaf), trachea cell diameter (leaf), adaxial stomatal index (leaf), trachea cell diameter (stem), and abaxial stomata length (leaf) resulted the most effective variables for the PCA. HCA dendrogram also revealed two main clusters. Determining the morphological and anatomical features of <em>Doronicum</em> taxa, clarifying the systematic value of anatomical features through numerical analysis and contributing to the systematic position of the examined taxa will be guiding for future studies.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"194 ","pages":"Article 103812"},"PeriodicalIF":2.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of sample misalignment and overlap on high-resolution quantification of 2D-metal heterostructures 样品错位和重叠对二维金属异质结构高分辨率定量的影响

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-13 DOI: 10.1016/j.micron.2025.103813

Yuchen Zhu, Rongming Wang

{"title":"The impact of sample misalignment and overlap on high-resolution quantification of 2D-metal heterostructures","authors":"Yuchen Zhu, Rongming Wang","doi":"10.1016/j.micron.2025.103813","DOIUrl":"10.1016/j.micron.2025.103813","url":null,"abstract":"<div><div>Precise atomic-scale fabrication of two-dimensional (2D) semiconductor metal heterostructures necessitates an in-depth understanding of their surface atomic arrangements, interfacial atomic configurations, and electronic state. The foundation for achieving this understanding lies in the accurate characterization of these heterostructures. Aberration-corrected transmission electron microscopy (ACTEM) is capable of simultaneously obtaining critical information regarding atomic arrangements, electronic states, and elemental distributions at the atomic scale, making it widely utilized for the analysis and characterization of heterostructures. However, the accuracy of ACTEM can be compromised by sample overlaps in high-resolution images, a challenge particularly pronounced in 2D-semiconductor-metal heterostructures. To address this, we selected Pt-MoS₂ as a model system and systematically investigated the effects of misalignment and sample overlap on quantitative electron microscopy analysis of two-dimensional semiconductor heterostructures through high-resolution image simulations. Our findings reveal that even small misalignments (< 2°) introduce errors of approximately ∼2 % in the analysis of interplanar spacing. Furthermore, the overlap of the MoS₂ potential function can induce apparent strain (>5 %) in high-resolution TEM (HRTEM) image. Notably, the apparent strain from potential function overlap remains within an acceptable range only when the relative thickness ratio of metal to 2D substrate exceeds a certain threshold, improving the reliability of direct quantitative analysis of HRTEM images. Additionally, we applied a digital dark-field method that effectively mitigates the influence of the substrate on metal atomic column contrasts, thus improving the accuracy of quantitative analysis in HRTEM images. Collectively, these results provide a deeper understanding of the heterostructure imaging and the precise characterization of the 2D-material-metal heterostructure.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103813"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examination of paraffin-embedded testes of domestic cats and dogs by light and scanning electron microscopy 家用猫和狗石蜡包埋睾丸的光镜和扫描电镜检查

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-11 DOI: 10.1016/j.micron.2025.103816

Banu Kandil , Beste Demirci , Alev Gürol Bayraktaroğlu , Emre Demirci

{"title":"Examination of paraffin-embedded testes of domestic cats and dogs by light and scanning electron microscopy","authors":"Banu Kandil , Beste Demirci , Alev Gürol Bayraktaroğlu , Emre Demirci","doi":"10.1016/j.micron.2025.103816","DOIUrl":"10.1016/j.micron.2025.103816","url":null,"abstract":"<div><div>This study was designed to determine the appropriate section thickness for SEM analysis of paraffin-embedded testes. This study used paraffin-embedded testes from 6 adult cats and 6 adult dogs. Tissue blocks were cut into 10, 20, and 40 µm thick sections and analyzed by SEM. In addition, seminiferous tubule and lumen diameter and germinative epithelium height were measured in 10 and 20 µm thick paraffin sections. Measurements from 10 and 20 µm thick paraffin sections showed that the height of the germinative epithelium and the diameter of the lumen and tubules were similar between cats and dogs (p > 0.05). In cats and dogs, tubule diameter and germinative epithelium height were higher in the 10 µm thick paraffin sections compared to the 20 µm thick paraffin sections. However, in both species, the lumen diameter was greater in the 20 µm thick paraffin sections than in the 10 µm thick paraffin sections (p < 0.05). Unlike 40 µm thick paraffin sections, 10 and 20 µm thick paraffin sections allowed the structural features of the testes to be examined. Additionally, 10 µm thick paraffin sections were ideal for histometric measurements, while 20 µm thick paraffin sections were suitable for detailed examination of late spermatids. The present study showed that testes may be examined in detail by SEM from 10 and 20 µm thick paraffin sections. In addition, this study may contribute to the design of new studies to reveal the missing three-dimensional structures of paraffin-embedded testicular tissues stored in tissue archives by analyzing them by SEM.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103816"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suberin nanostructures of Malus domestica Borkh. (Rosaceae) pericarp 家槐木质素的纳米结构。(蔷薇科)果皮

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-11 DOI: 10.1016/j.micron.2025.103815

Tamara Kh. Kumachova , Alexander S. Voronkov

{"title":"Suberin nanostructures of Malus domestica Borkh. (Rosaceae) pericarp","authors":"Tamara Kh. Kumachova , Alexander S. Voronkov","doi":"10.1016/j.micron.2025.103815","DOIUrl":"10.1016/j.micron.2025.103815","url":null,"abstract":"<div><div>New data are presented on nanosized suberin structures that directly participate in the formation of suberin deposits into the cell walls of the <em>Malus domestica</em> outer pericarp zone. The nanostructures were spherical formations containing conglomerates of electron-dense nanoparticles (diameter 5–30 nm) in the center. It has been demonstrated for the first time that the site of synthesis of suberin monomers, along with the endoplasmic reticulum (ER), may be the chloroplasts of hypoderm cells. Several possibilities for the transport of suberin nanoparticles have been discovered: via ER cisterns and its subdomains, via Golgi apparatus microvesicles, and via plasma membrane invaginations. The membrane structures of these compartments are directly related to intracytosis, the intracellular movement of suberin monomers; they can carry suberin monomeric precursors of a lipid nature in the membranes themselves or in the tubule’s lumen. Either way, fusion with the hydrophobic surface of the suberin plate releases monomers, and they become accessible to apoplastic enzymes that polymerize suberin. It cannot be ruled out that the extremely arid conditions of the steppe ecological zone where the <em>M. domestica</em> grew could have influenced the features of metabolic processes, in particular, activating all possible pathways for the formation of suberin monomers, which make up suberin nanostructures.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103815"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology of the male reproductive apparatus and sperm ultrastructure of Thaumastocoris peregrinus Carpintero & Dellape 2006 (Heteroptera: Thaumastocoridae) 男性生殖装置和精子超微结构的形态学Thaumastocoris peregrinus Carpintero & Dellape 2006(半翅目:Thaumastocoridae)

IF 2.5 3区工程技术

Micron Pub Date : 2025-03-07 DOI: 10.1016/j.micron.2025.103811

Paulo Henrique Rezende , Dayvson Ayala-Costa , Mauricio da Silva Paulo , Glenda Dias , José Lino-Neto

{"title":"Morphology of the male reproductive apparatus and sperm ultrastructure of Thaumastocoris peregrinus Carpintero & Dellape 2006 (Heteroptera: Thaumastocoridae)","authors":"Paulo Henrique Rezende , Dayvson Ayala-Costa , Mauricio da Silva Paulo , Glenda Dias , José Lino-Neto","doi":"10.1016/j.micron.2025.103811","DOIUrl":"10.1016/j.micron.2025.103811","url":null,"abstract":"<div><div>In this study, we examine the male reproductive apparatus and sperm morphology of <em>Thaumastocoris peregrinus</em> (Thaumastocoridae), a pest of <em>Eucalyptus</em> plantations, to explore its phylogenetic implications using light and transmission electron microscopy. The reproductive apparatus comprises paired testes with three follicles each, dilated efferent ducts acting as seminal vesicles, paired accessory glands, and ejaculatory duct that connects with an aedeagus. Sperm are filiform, around 465 µm long, with short, fusiform nuclei (< 6 µm) and an acrosome observable only under TEM. Key synapomorphies of Heteroptera, including symmetrical mitochondrial derivatives with paracrystalline inclusions and axoneme-derivative bridges, are present. The testicular follicles had mostly late spermatids and no evident differentiation zone, which was different from previously described. These distinctive features may support the recent phylogenetic placement of Thaumastocoridae as a sister group to Pentatomomorpha.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103811"},"PeriodicalIF":2.5,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing TEM experiments with thin lamella all-solid-state cells in controlled atmosphere using electrochemical chips 利用电化学芯片设计可控气氛下薄片全固态电池的透射电镜实验

IF 2.5 3区工程技术

Micron Pub Date : 2025-02-28 DOI: 10.1016/j.micron.2025.103810

Paul Naillou , Adrien Boulineau , Sami Oukassi , Philippe Azaïs

{"title":"Designing TEM experiments with thin lamella all-solid-state cells in controlled atmosphere using electrochemical chips","authors":"Paul Naillou , Adrien Boulineau , Sami Oukassi , Philippe Azaïs","doi":"10.1016/j.micron.2025.103810","DOIUrl":"10.1016/j.micron.2025.103810","url":null,"abstract":"<div><div>While <em>in situ</em> techniques in transmission electron microscopy (TEM) field provide increasingly more useful and accurate data on various physical phenomena, electrochemical reactions occurring on interfaces in all-solid-state lithium-ion batteries (ASSLIB) still remain incompletely understood. High resolution and time-resolved TEM observations of interfaces stand as the pinnacle of battery research, but current most promising solid electrolyte materials exhibit major inconveniences for standard characterization protocols such as ambient air sensitivity. While several groups have developed methods to study lithium-ion cells in <em>in situ</em> TEM, mostly through thin lamella preparation, few seemed to have kept the sample in controlled atmosphere. Yet, this can stand as a critical criterion for maintaining material integrity during transfer and acquisition. In this work, we describe our attempt to lead <em>operando-</em>TEM characterization on a thin lamella all-solid-state lithium-ion cell by a new technique involving the use of a specific controlled-atmosphere sample holder and electrochemical chips including electron-transparent windows. Achievements, challenges and prospects of this method are discussed here.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103810"},"PeriodicalIF":2.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous recording of the surface and internal structures of helminth parasites by fluorescence stereomicroscopy and light-sheet fluorescence microscopy (LSFM) 利用荧光立体显微镜和光片荧光显微镜（LSFM）同时记录寄生虫表面和内部结构

IF 2.5 3区工程技术

Micron Pub Date : 2025-02-20 DOI: 10.1016/j.micron.2025.103802

Ana Paula Nascimento Gomes , Kildare Miranda , Eduardo José Lopes-Torres

{"title":"Simultaneous recording of the surface and internal structures of helminth parasites by fluorescence stereomicroscopy and light-sheet fluorescence microscopy (LSFM)","authors":"Ana Paula Nascimento Gomes , Kildare Miranda , Eduardo José Lopes-Torres","doi":"10.1016/j.micron.2025.103802","DOIUrl":"10.1016/j.micron.2025.103802","url":null,"abstract":"<div><div>The study of helminth parasites has been carried out using different microscopy tools such as bright-field, fluorescence, confocal, and electron microcopies, providing morphological and ultrastructural information, which are important for morphological taxonomy. However, these microscopy techniques are unable to visualize both surface and internal structures simultaneously at high resolution in a single analysis. Consequently, researchers must use different tools to enhance the knowledge of helminths and other parasites. The present work highlights the importance of using a fluorescence stereomicroscopy based in the structured illumination to visualize simultaneously the surface and internal structures of helminth and other metazoan samples. Our results using a single equipment showed the surface topography and internal structures of the whole parasite simultaneously. In addition, the series of images can be applied to produce a three-dimensional (3D) model of the samples. These advanced methods can indeed open new frontiers for obtaining better morphological data, enriching the knowledge in helminthology, and enhancing studies of other invertebrates, especially where thick samples, which are common, are present. Recent studies with large and thick helminths have provided 2D and 3D visualization of the whole parasite. This represents an important advance in the investigation of helminth parasitology, invertebrate morphophysiology, and other areas of microanatomy study in metazoans.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"192 ","pages":"Article 103802"},"PeriodicalIF":2.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular dynamics study on the mitigation of radiation damage caused by electron pulses 减轻电子脉冲辐射损伤的分子动力学研究

IF 2.5 3区工程技术

Micron Pub Date : 2025-02-13 DOI: 10.1016/j.micron.2025.103801

Wenyan Zhao , Peng Wu , Rui Xu , Zhuangzhi Li , Huanxin Yang , Chunhui Zhu , Jianqi Li

{"title":"Molecular dynamics study on the mitigation of radiation damage caused by electron pulses","authors":"Wenyan Zhao , Peng Wu , Rui Xu , Zhuangzhi Li , Huanxin Yang , Chunhui Zhu , Jianqi Li","doi":"10.1016/j.micron.2025.103801","DOIUrl":"10.1016/j.micron.2025.103801","url":null,"abstract":"<div><div>The reduction of radiation damage represents a long-term objective for electron microscopists, particularly those engaged in the study of biological and organic matter. Recently, electron pulses in ultrafast transmission electron microscopy have been demonstrated to serve as a damage mitigation technique for radiation-sensitive materials. Nevertheless, the underlying mechanism of the mitigation effects remains unclear. In this study, we investigate the radiation damage of graphene induced by pulsed electrons using molecular dynamics simulations within the framework of binary elastic collisions. For electron irradiation at 200 keV, it was found that the pulsed electron beam corresponds to a larger threshold angle (1.4 rad) than that for a random beam (1.0 rad). This is because two electrons can be prevented from briefly interacting with the same or a neighboring atom by the use of well-controlled electron pulses. While such a mitigation of radiation damage is only apparent near the threshold angle, and there are likely other reduction mechanisms, our results provide insight into the mitigated radiation damage of electron pulses.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"191 ","pages":"Article 103801"},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143403286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of the trophic chamber and follicular epithelium in ovaries of the model heteropteran species Pyrrhocoris apterus 异翅鸟模型种翼红蛾卵巢营养腔和卵泡上皮的结构

IF 2.5 3区工程技术

Micron Pub Date : 2025-02-10 DOI: 10.1016/j.micron.2025.103787

Anna Szczepankiewicz , Bożena Simiczyjew

{"title":"Structure of the trophic chamber and follicular epithelium in ovaries of the model heteropteran species Pyrrhocoris apterus","authors":"Anna Szczepankiewicz , Bożena Simiczyjew","doi":"10.1016/j.micron.2025.103787","DOIUrl":"10.1016/j.micron.2025.103787","url":null,"abstract":"<div><div>The studies concern organization of the female gonads and the course of oogenesis in the model species of Heteroptera, <em>Pyrrhocoris apterus</em>. Morphological, cytochemical and ultrastructural analyses were carried out. Each of the paired ovaries of the studied bug comprises seven telotrophic ovarioles. An individual ovariole is composed of the terminal filament, tropharium, vitellarium, and ovariole pedicle. The tropharium houses morphologically diversified trophocytes. In the apical part small individual nurse cells are located, some of them are mitotically active. Below this zone nuclei of the trophocytes divide amitotically. The main part of the trophic chamber is composed of cytoplasmic lobes containing several trophocyte nuclei. Each lobe connects with the trophic core by cytoplasmic extension. In the basal part of the tropharium early previtellogenic oocytes and somatic prefollicular cells occur. The vitellarium houses oocytes at different developmental stages, surrounded by follicular cells, with younger oocytes positioned apically and older ones basally. The contact between oocytes and trophocytes is maintained by nutritive cords filled with densely packed microtubules. Numerous ribosomes and mitochondria occur within the cords. The follicular epithelium undergoes a series of changes and diversifies into three subpopulations. The general organization of <em>P. apterus</em> ovarioles is similar to that described in other representatives of Heteroptera. The differences concern the structure of the tropharium, the number and growth rate of ovarian follicles and the course of differentiation of the follicular epithelium.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"191 ","pages":"Article 103787"},"PeriodicalIF":2.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Y and Zr addition on the intergranular corrosion of Al-Mg alloy after sensitization treatment 添加Y和Zr对铝镁合金敏化后晶间腐蚀的影响

IF 2.5 3区工程技术

Micron Pub Date : 2025-02-08 DOI: 10.1016/j.micron.2025.103789

Zhiqiang Ru , Lili Wei , Ming Liu , Zhimin Pi , Chang Han , Ming Qiu , Enbang Li , Hongfeng Huang , Degui Li

引用次数: 0

Micron最新文献