Memorias do Instituto Oswaldo Cruz最新文献_第8页

Histone deacetylase (HDAC) inhibitors- based drugs are effective to control Mycobacterium tuberculosis infection and promote the sensibility for rifampicin in MDR strain. 以组蛋白去乙酰化酶（HDAC）抑制剂为基础的药物可有效控制结核分枝杆菌感染，并提高耐药菌株对利福平的敏感性。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-12-22 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230143

Adrián Rodríguez-Carlos, Yolanda Jacobo-Delgado, Alan Orlando Santos-Mena, Mariana H García-Hernández, Luis Adrian De Jesus-Gonzalez, Edgar E Lara-Ramirez, Bruno Rivas-Santiago

{"title":"Histone deacetylase (HDAC) inhibitors- based drugs are effective to control Mycobacterium tuberculosis infection and promote the sensibility for rifampicin in MDR strain.","authors":"Adrián Rodríguez-Carlos, Yolanda Jacobo-Delgado, Alan Orlando Santos-Mena, Mariana H García-Hernández, Luis Adrian De Jesus-Gonzalez, Edgar E Lara-Ramirez, Bruno Rivas-Santiago","doi":"10.1590/0074-02760230143","DOIUrl":"10.1590/0074-02760230143","url":null,"abstract":"Background: Tuberculosis (TB) is a major public health problem, which has been aggravated by the alarming growth of drug-resistant tuberculosis. Therefore, the development of a safer and more effective treatment is needed.Objectives: The aim of this work was repositioning and evaluate histone deacetylases (HDAC) inhibitors- based drugs with potential antimycobacterial activity.Methods: Using an in silico pharmacological repositioning strategy, three molecules that bind to the catalytic site of histone deacetylase were selected. Pneumocytes type II and macrophages were infected with Mycobacterium tuberculosis and treated with pre-selected HDAC inhibitors (HDACi). Subsequently, the ability of each of these molecules to directly promote the elimination of M. tuberculosis was evaluated by colony-forming unit (CFU)/mL. We assessed the expression of antimicrobial peptides and respiratory burst using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Findings: Aminoacetanilide (ACE), N-Boc-1,2-phenylenediamine (N-BOC), 1,3-Diphenylurea (DFU), reduce bacillary loads in macrophages and increase the production of β-defensin-2, LL-37, superoxide dismutase (SOD) 3 and inducible nitric oxide synthase (iNOS). While only the use of ACE in type II pneumocytes decreases the bacterial load through increasing LL-37 expression. Furthermore, the use of ACE and rifampicin inhibited the survival of intracellular multi-drug resistance M. tuberculosis.Main conclusions: Our data support the usefulness of in silico approaches for drug repositioning to provide a potential adjunctive therapy for TB.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230143"},"PeriodicalIF":2.8,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10740574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictors of Trypanosoma cruzi PCR positivity in patients with chronic Chagas disease. 慢性恰加斯病患者体内克氏锥虫 PCR 阳性的预测因素。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-12-15 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230115

Ana Carolina Bastos de Lima, Veronica Gonçalves Mendes, Roberto Rodrigues Ferreira, Lindice Mitie Nisimura, Samuel Iwao Maia Horita, Henrique H Veloso, Andréa R Costa, Gilberto Marcelo S da Silva, Luiz Henrique C Sangenis, Marcelo T Holanda, Lorena Rimolo, Ademir B Cunha, Luciana Ribeiro Garzoni, Alejandro Marcel Hasslocher-Moreno, Mauro Felippe F Mediano, Otacílio da Cruz Moreira, Constança Britto, Roberto M Saraiva

{"title":"Predictors of Trypanosoma cruzi PCR positivity in patients with chronic Chagas disease.","authors":"Ana Carolina Bastos de Lima, Veronica Gonçalves Mendes, Roberto Rodrigues Ferreira, Lindice Mitie Nisimura, Samuel Iwao Maia Horita, Henrique H Veloso, Andréa R Costa, Gilberto Marcelo S da Silva, Luiz Henrique C Sangenis, Marcelo T Holanda, Lorena Rimolo, Ademir B Cunha, Luciana Ribeiro Garzoni, Alejandro Marcel Hasslocher-Moreno, Mauro Felippe F Mediano, Otacílio da Cruz Moreira, Constança Britto, Roberto M Saraiva","doi":"10.1590/0074-02760230115","DOIUrl":"10.1590/0074-02760230115","url":null,"abstract":"Background: A positive Trypanosoma cruzi polymerase chain reaction (PCR) is associated with a worse prognosis in patients with chronic Chagas disease (CD).Objectives: To study the association of clinical, electrocardiographic, and echocardiographic characteristics and biomarker blood levels with positive T. cruzi PCR in chronic CD.Methods: This is a single-centre observational cross-sectional study. Positive T. cruzi PCR association with clinical, electrocardiographic, and echocardiographic characteristics, and biomarker blood levels were studied by logistic regression analysis. p values < 0.05 were considered significant.Findings: Among 333 patients with chronic CD (56.4% men; 62 ± 10 years), T. cruzi PCR was positive in 41.1%. Stepwise multivariate logistic regression showed an independent association between positive T. cruzi PCR and diabetes mellitus {odds ratio (OR) 0.53 [95% confidence interval (CI) 0.30-0.93]; p = 0.03}, right bundle branch block [OR 1.78 (95% CI 1.09-2.89); p = 0.02], and history of trypanocidal treatment [OR 0.13 (95% CI 0.04-0.38); p = 0.0002]. Among patients with a history of trypanocidal treatment (n = 39), only four (10%) patients had a positive T. cruzi PCR.Main conclusions: Among several studied parameters, only diabetes mellitus, right bundle branch block, and history of trypanocidal treatment showed an independent association with positive T. cruzi PCR. History of trypanocidal treatment was a strong protective factor against a positive T. cruzi PCR.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230115"},"PeriodicalIF":2.8,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10727046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, on Leishmania (Leishmania) amazonensis. 从Crotalus durissus collilineatus蛇血清中提取的磷脂酶A2抑制剂γCdcPLI对亚马孙利什曼原虫的抗利什曼原虫作用。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-11-27 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760220225

Marina Neves Gonçalves, Daiana Silva Lopes, Samuel Cota Teixeira, Thaise Lara Teixeira, Vitor de Freitas, Tássia Rafaella Costa, Sarah Natalie Cirilo Gimenes, Isabella Mitie de Camargo, Guilherme de Souza, Marcelo Santos da Silva, Fernanda Van Petten de Vasconcelos Azevedo, Kathleen Fernandes Grego, Luísa Carregosa Santos, Vinícius Queiroz Oliveira, Claudio Vieira da Silva, Renata Santos Rodrigues, Kelly Aparecida Geraldo Yoneyama, Patricia Bianca Clissa, Veridiana de Melo Rodrigues

{"title":"Antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, on Leishmania (Leishmania) amazonensis.","authors":"Marina Neves Gonçalves, Daiana Silva Lopes, Samuel Cota Teixeira, Thaise Lara Teixeira, Vitor de Freitas, Tássia Rafaella Costa, Sarah Natalie Cirilo Gimenes, Isabella Mitie de Camargo, Guilherme de Souza, Marcelo Santos da Silva, Fernanda Van Petten de Vasconcelos Azevedo, Kathleen Fernandes Grego, Luísa Carregosa Santos, Vinícius Queiroz Oliveira, Claudio Vieira da Silva, Renata Santos Rodrigues, Kelly Aparecida Geraldo Yoneyama, Patricia Bianca Clissa, Veridiana de Melo Rodrigues","doi":"10.1590/0074-02760220225","DOIUrl":"10.1590/0074-02760220225","url":null,"abstract":"Background: Leishmaniasis, a neglected disease caused by the parasite Leishmania, is treated with drugs associated with high toxicity and limited efficacy, in addition to constant reports of the emergence of resistant parasites. In this context, snake serums emerge as good candidates since they are natural sources with the potential to yield novel drugs.Objectives: We aimed to show the antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, against Leishmania (Leishmania) amazonensis.Methods: Promastigotes forms were exposed to γCdcPLI, and we assessed the parasite viability and cell cycle, as well as invasion and proliferation assays.Findings: Despite the low cytotoxicity effect on macrophages, our data indicate that γCdcPLI has a direct effect on parasites promoting an arrest in the G1 phase and reduction in the G2/M phase at the highest dose tested. Moreover, this PLA2 inhibitor reduced the parasite infectivity when promastigotes were pre-treated. Also, we demonstrated that the γCdcPLI treatment modulated the host cell environment impairing early and late steps of the parasitism.Main conclusions: γCdcPLI is an interesting tool for the discovery of new essential targets on the parasite, as well as an alternative compound to improve the effectiveness of the leishmaniasis treatment.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220225"},"PeriodicalIF":2.8,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique synapomorphies and high diversity in South American Raji-related Epstein-Barr virus genomes. 南美Raji相关Epstein-Barr病毒基因组中独特的突触形态和高度多样性。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-11-03 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230122

Paula Alves, Vanessa Emmel, Gustavo Stefanoff, Flavia Krsticevic, Joaquín Ezpeleta, Javier Murillo, Elizabeth Tapia, Edson Delatorre, Eliana Abdelhay, Rocio Hassan

{"title":"Unique synapomorphies and high diversity in South American Raji-related Epstein-Barr virus genomes.","authors":"Paula Alves, Vanessa Emmel, Gustavo Stefanoff, Flavia Krsticevic, Joaquín Ezpeleta, Javier Murillo, Elizabeth Tapia, Edson Delatorre, Eliana Abdelhay, Rocio Hassan","doi":"10.1590/0074-02760230122","DOIUrl":"10.1590/0074-02760230122","url":null,"abstract":"Background: Epstein-Barr virus (EBV) is a human gammaherpesvirus etiologically linked to several benign and malignant diseases. EBV-associated malignancies exhibit an unusual global distribution that might be partly attributed to virus and host genetic backgrounds.Objectives: To assemble a new genome of EBV (CEMO3) from a paediatric Burkitt's lymphoma from Rio de Janeiro State (Southeast Brazil). In addition, to perform global phylogenetic analysis using complete EBV genomes, including CEMO3, and investigate the genetic relationship of some South American (SA) genomes through EBV subgenomic targets.Methods: CEMO3 was sequenced through next generation sequencing and its coverage and gaps were corrected through the Sanger method. CEMO3 and 67 EBV genomes representing diverse geographic regions were evaluated through maximum likelihood phylogenetic analysis. Further, the polymorphism of subgenomic regions of some SA EBV genomes were assessed.Findings: The whole bulk tumour sequencing yielded 23,217 reads related to EBV, which 172,713 base pairs of the newly EBV genome CEMO3 was assembled. The CEMO3 and most SA EBV genomes clustered within the SA subclade closely related to the African Raji strain, forming the South American/Raji clade. Notably, these Raji-related genomes exhibit significant genetic diversity, characterised by distinctive synapomorphies at some gene levels absent in the original Raji strain.Conclusion: The CEMO3 represents a new South American EBV genome assembled. Albeit the majority of EBV genomes from SA are Raji-related, it harbours a high diversity different from the original Raji strain.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230122"},"PeriodicalIF":2.8,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71483359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the genome of a high-risk pandrug-resistant Klebsiella pneumoniae emerging in the Brazilian Amazon Region, 2022. 2022年，公布巴西亚马逊地区出现的高风险耐泛药肺炎克雷伯菌的基因组。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-27 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230081

Érica Lourenço Fonseca, Sérgio M Morgado, Fernanda S Freitas, Nathalia S Bighi, Rosângela Cipriano, Ana Carolina Paulo Vicente

{"title":"Unveiling the genome of a high-risk pandrug-resistant Klebsiella pneumoniae emerging in the Brazilian Amazon Region, 2022.","authors":"Érica Lourenço Fonseca, Sérgio M Morgado, Fernanda S Freitas, Nathalia S Bighi, Rosângela Cipriano, Ana Carolina Paulo Vicente","doi":"10.1590/0074-02760230081","DOIUrl":"10.1590/0074-02760230081","url":null,"abstract":"Background: Pandrug-resistant (PDR) Klebsiella pneumoniae has been reported sporadically in many countries and remains rare in Brazil.Objectives: This study unravelled the genetic determinants involved with the PDR background of a clinical ST11 K. pneumoniae recovered in the Brazilian Amazon Region, where K. pneumoniae genomic and epidemiological information is scarce.Methods: Kp196 was submitted to the antimicrobial susceptibility test by the disk-diffusion method and minimum inhibitory concentration (MIC) determination. The whole genome sequencing was obtained and the sequence type was determined by core genome multilocus sequence typing (cgMLST). Its intrinsic and acquired resistome was assessed by Comprehensive Antibiotic Resistance Database (CARD) and comparison with wild-type genes.Findings: The analyses revealed that Kp196 belonged to the pandemic ST11 and presented the PDR phenotype. Its acquired resistome was composed of a huge set of clinically relevant resistance determinants, including bla CTX-M-15 and bla NDM-1, all found in the vicinity of mobile platforms. Considering its intrinsic resistome, the multidrug resistance, especially to colistin, tigecycline and fluoroquinolones, was multifactorial and attributed to modifications (indels, missense mutations, and gene disruption) in several housekeeping genes (arnT/phoQ/mgrB/ramR/acrB/gyrA/parC/ompK35-36-37). The Kp196 intrinsic resistome was also observed in a ST11 environmental strain, although harbouring distinct acquired resistomes.Conclusions: An accumulation of different resistance mechanisms regarding the intrinsic resistome accounts for a more stable resistome, strongly contributing to the Kp196 PDR phenotype.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230081"},"PeriodicalIF":2.8,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71424881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benznidazole treatment decreases IL-6 levels in Trypanosoma cruzi-infected human adipocytes differentiated from adipose tissue-derived stem cells. 苯硝唑治疗降低了从脂肪组织衍生的干细胞分化的感染克氏锥虫的人类脂肪细胞中的IL-6水平。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-23 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760220295

Leyllane Rafael Moreira, Ana Carla Silva, Cíntia Nascimento da Costa Oliveira, Claudeir Dias da Silva Júnior, Amanda Vasconcelos Nascimento, Kamila Kássia Dos Santos Oliveira, Ana Karine de Araújo Soares, Karina Lidianne Alcântara Saraiva, Milena de Paiva Cavalcanti, Virginia Maria Barros de Lorena

{"title":"Benznidazole treatment decreases IL-6 levels in Trypanosoma cruzi-infected human adipocytes differentiated from adipose tissue-derived stem cells.","authors":"Leyllane Rafael Moreira, Ana Carla Silva, Cíntia Nascimento da Costa Oliveira, Claudeir Dias da Silva Júnior, Amanda Vasconcelos Nascimento, Kamila Kássia Dos Santos Oliveira, Ana Karine de Araújo Soares, Karina Lidianne Alcântara Saraiva, Milena de Paiva Cavalcanti, Virginia Maria Barros de Lorena","doi":"10.1590/0074-02760220295","DOIUrl":"10.1590/0074-02760220295","url":null,"abstract":"Background: Trypanosoma cruzi, which causes Chagas disease (CD), is a versatile haemoparasite that uses several strategies to evade the host's immune response, including adipose tissue (AT), used as a reservoir of infection. As it is an effective barrier to parasite evasion, the effectiveness of the drug recommended for treating CD, Benznidazole (BZ), may be questionable.Objective: To this end, we evaluated the parasite load and immunomodulation caused by BZ treatment in the culture of adipocytes differentiated from human adipose tissue-derived stem cells (ADSC) infected with T. cruzi.Methods: The ADSC were subjected to adipogenic differentiation. We then carried out four cultures in which we infected the differentiated AT with trypomastigote forms of the Y strain of T. cruzi and treated them with BZ. After the incubation, the infected AT was subjected to quantitative polymerase chain reaction (qPCR) to quantify the parasite load and transmission electron microscopy (TEM) to verify the infection. The supernatant was collected to measure cytokines, chemokines, and adipokines.Findings: We found elevated secretion of IL-6, CXCL-10/IP-10, CCL2/MCP-1, CCL5/RANTES, and leptin in infected fat cells. However, treatment with BZ promoted a decrease in IL-6.Main conclusion: Therefore, we believe that BZ has a beneficial role as it reduces inflammation in infected fat cells.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220295"},"PeriodicalIF":2.8,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50162134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relevance of national, regional and global virome projects on pandemics prediction, prevention, and control: a social network analysis of GVP-citing articles. 国家、区域和全球病毒组项目在流行病预测、预防和控制方面的相关性：GVP引用文章的社会网络分析。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-20 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230116

Bruna de Paula Fonseca, Carlos Medicis Morel

{"title":"Relevance of national, regional and global virome projects on pandemics prediction, prevention, and control: a social network analysis of GVP-citing articles.","authors":"Bruna de Paula Fonseca, Carlos Medicis Morel","doi":"10.1590/0074-02760230116","DOIUrl":"10.1590/0074-02760230116","url":null,"abstract":"Background: The Global Virome Project (GVP) was proposed in 2018 as an evolution of the USAID PREDICT project and was presented as a \"collaborative scientific initiative to discover zoonotic viral threats and stop future pandemics\". The immediate response was mixed, with public health and scientific communities representatives showing skepticism, if not direct opposition.Objectives: The economic, social, and health consequences of the coronavirus disease 2019 (COVID-19) pandemic demonstrated how unprepared the world was in the face of new pandemics. This paper analyses the impact of the GVP on the scientific and public health communities.Methods: Published scientific articles that cited the two 2018 seminal publications proposing the project were analysed using social network analysis methods.Findings: Encompassing the periods before and after the onset of the Covid-19 pandemic, the results indicate that (i) the concepts of the GVP have received more support than opposition in the scientific literature; (ii) its foundations should be updated to address the specific criticisms.Main conclusions: Shifting focus to national virome projects can provide tangible, regional benefits that can positively contribute towards a consensus on achieving a high level of preparedness for the ever-present possibility of the following global viral pandemic.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230116"},"PeriodicalIF":2.8,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50162135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in responses to the intracellular macrophage environment between Mycobacterium bovis BCG vaccine strains Moreau and Pasteur. 牛分枝杆菌BCG疫苗株Moreau和巴斯德对细胞内巨噬细胞环境反应的差异。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-13 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230070

Paloma Rezende Corrêa, Marcos Gustavo Araujo Schwarz, Renata Monteiro Maia, Fátima Maria Figueroa Vergara, Milton Ozório Moraes, Leila Mendonça-Lima

{"title":"Differences in responses to the intracellular macrophage environment between Mycobacterium bovis BCG vaccine strains Moreau and Pasteur.","authors":"Paloma Rezende Corrêa, Marcos Gustavo Araujo Schwarz, Renata Monteiro Maia, Fátima Maria Figueroa Vergara, Milton Ozório Moraes, Leila Mendonça-Lima","doi":"10.1590/0074-02760230070","DOIUrl":"10.1590/0074-02760230070","url":null,"abstract":"Background: The Bacille Calmette-Guérin (BCG) vaccine comprises a family of strains with variable protective efficacy against pulmonary tuberculosis (TB) and leprosy, partly due to genetic differences between strains.Objectives: Previous data highlighting differences between the genomes and proteomic profiles of BCG strains Moreau and Pasteur led us to evaluate their behaviour in the macrophage microenvironment, capable of stimulating molecular responses that can impact the protective effect of the vaccine.Methods: Strain infectivity, viability, co-localisation with acidified vesicles, macrophage secretion of IL-1 and MCP-1 and lipid droplet biogenesis were evaluated after infection.Findings: We found that BCG Moreau is internalised more efficiently, with significantly better intracellular survival up to 96 h p.i., whereas more BCG Pasteur bacilli were found co-localised in acidified vesicles up to 6 h p.i. IL-1β and MCP-1 secretion and lipid droplet biogenesis by infected macrophages were more prominent in response to BCG Pasteur.Main conclusion: Overall, our results show that, compared to Pasteur, BCG Moreau has increased fitness and better endurance in the harsh intracellular environment, also regulating anti-microbial responses (lower IL-1b and MCP-1). These findings contribute to the understanding of the physiology of BCG Moreau and Pasteur in response to the intraphagosomal environment in a THP-1 macrophage model.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230070"},"PeriodicalIF":2.8,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49679342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long COVID-19 syndrome associated with Omicron XBB.1.5 infection: a case report. 长期新冠肺炎综合征与奥密克戎XBB.1.5感染：病例报告。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-13 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230069

Otávio Espíndola, Paola C Resende, Lusiele Guaraldo, Guilherme Amaral Calvet, Trevon L Fuller, Stephanie Lema Suarez Penetra, Heloisa Ferreira Pinto Santos, Anielle Pina-Costa, Michele Fernanda Borges da Silva, Isabella Campos Vargas Moraes, Fernando Medeiros, Jimmy Whitworth, Christopher Smith, Karin Nielsen-Saines, Marilda M Siqueira, Patrícia Brasil

{"title":"Long COVID-19 syndrome associated with Omicron XBB.1.5 infection: a case report.","authors":"Otávio Espíndola, Paola C Resende, Lusiele Guaraldo, Guilherme Amaral Calvet, Trevon L Fuller, Stephanie Lema Suarez Penetra, Heloisa Ferreira Pinto Santos, Anielle Pina-Costa, Michele Fernanda Borges da Silva, Isabella Campos Vargas Moraes, Fernando Medeiros, Jimmy Whitworth, Christopher Smith, Karin Nielsen-Saines, Marilda M Siqueira, Patrícia Brasil","doi":"10.1590/0074-02760230069","DOIUrl":"10.1590/0074-02760230069","url":null,"abstract":"Background: There is interest in lingering non-specific symptoms after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, referred to as Long coronavirus disease 2019 (Long COVID-19). It remains unknown whether the risk of Long COVID-19 is associated with pre-existing comorbidities or initial COVID-19 severity, including infections due to new Omicron lineages which predominated in 2023.Objectives: The aim of this case report was to characterize the clinical features of acute XBB.1.5 infection followed by Long COVID-19.Methods: We followed a 73-year old female resident of Rio de Janeiro with laboratory-confirmed SARS-CoV-2 during acute infection and subsequent months. The SARS-CoV-2 lineage was determined by genome sequencing.Findings: The participant denied comorbidities and had completed a two-dose vaccination schedule followed by two booster doses eight months prior to SARS-CoV-2 infection. Primary infection by viral lineage XBB.1.5. was clinically mild, but the participant subsequently reported persistent fatigue.Main conclusions: This case demonstrates that Long COVID-19 may develop even after mild disease due to SARS-CoV-2 in fully vaccinated and boosted individuals without comorbidities. Continued monitoring of new SARS-CoV-2 lineages and associated clinical outcomes is warranted. Measures to prevent infection should continue to be implemented including development of new vaccines and antivirals effective against novel variants.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230069"},"PeriodicalIF":2.8,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49679343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid droplets in Zika neuroinfection: Potential targets for intervention? 寨卡病毒神经感染中的脂滴：干预的潜在靶点？

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-09 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230044

Suelen Silva Gomes Dias, Tamires Cunha-Fernandes, Vinicius Cardoso Soares, Cecília Jg de Almeida, Patricia T Bozza

{"title":"Lipid droplets in Zika neuroinfection: Potential targets for intervention?","authors":"Suelen Silva Gomes Dias, Tamires Cunha-Fernandes, Vinicius Cardoso Soares, Cecília Jg de Almeida, Patricia T Bozza","doi":"10.1590/0074-02760230044","DOIUrl":"https://doi.org/10.1590/0074-02760230044","url":null,"abstract":"Lipid droplets (LD) are evolutionarily conserved lipid-enriched organelles with a diverse array of cell- and stimulus-regulated proteins. Accumulating evidence demonstrates that intracellular pathogens exploit LD as energy sources, replication sites, and part of the mechanisms of immune evasion. Nevertheless, LD can also favor the host as part of the immune and inflammatory response to pathogens. The functions of LD in the central nervous system have gained great interest due to their presence in various cell types in the brain and for their suggested involvement in neurodevelopment and neurodegenerative diseases. Only recently have the roles of LD in neuroinfections begun to be explored. Recent findings reveal that lipid remodelling and increased LD biogenesis play important roles for Zika virus (ZIKV) replication and pathogenesis in neural cells. Moreover, blocking LD formation by targeting DGAT-1 in vivo inhibited virus replication and inflammation in the brain. Therefore, targeting lipid metabolism and LD biogenesis may represent potential strategies for anti-ZIKV treatment development. Here, we review the progress in understanding LD functions in the central nervous system in the context of the host response to Zika infection.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230044"},"PeriodicalIF":2.8,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10566564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41204764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0