Memorias do Instituto Oswaldo Cruz最新文献

{"title":"Cerebrospinal fluid: a target of some fungi and an overview.","authors":"Danielly Corrêa-Moreira,&nbsp;Rodolfo Castro,&nbsp;Gisela Lara da Costa,&nbsp;Reginaldo Gonçalves Lima-Neto,&nbsp;Manoel Marques Evangelista Oliveira","doi":"10.1590/0074-02760220251","DOIUrl":"https://doi.org/10.1590/0074-02760220251","url":null,"abstract":"<p><p>Meningitis is a potentially life-threatening infection characterised by the inflammation of the leptomeningeal membranes. The estimated annual prevalence of 8.7 million cases globally and the disease is caused by many different viral, bacterial, and fungal pathogens. Although several genera of fungi are capable of causing infections in the central nervous system (CNS), the most significant number of registered cases have, as causal agents, yeasts of the genus Cryptococcus. The relevance of cryptococcal meningitis has changed in the last decades, mainly due to the increase in the number of people living with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) and medications that impair the immune responses. In this context, coronavirus disease 19 (COVID-19) has also emerged as a risk factor for invasive fungal infections (IFI), including fungal meningitis (FM), due to severe COVID-19 disease is associated with increased pro-inflammatory cytokines, interleukin (IL)-1, IL-6, and tumour necrosis factor-alpha, reduced CD4-interferon-gamma expression, CD4 and CD8 T cells. The gold standard technique for fungal identification is isolating fungi in the culture of the biological material, including cerebrospinal fluid (CSF). However, this methodology has as its main disadvantage the slow or null growth of some fungal species in culture, which makes it difficult to finalise the diagnosis. In conclusions, this article, in the first place, point that it is necessary to accurately identify the etiological agent in order to assist in the choice of the therapeutic regimen for the patients, including the implementation of actions that promote the reduction of the incidence, lethality, and fungal morbidity, which includes what is healthy in the CNS.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220251"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9174061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of gut-peripheral nervous system axis on the development of diabetic neuropathy.","authors":"Thalita Mázala-de-Oliveira,&nbsp;Yago Amigo Pinho Jannini de Sá,&nbsp;Vinicius de Frias Carvalho","doi":"10.1590/0074-02760220197","DOIUrl":"https://doi.org/10.1590/0074-02760220197","url":null,"abstract":"<p><p>Diabetes is a chronic metabolic disease caused by a reduction in the production and/or action of insulin, with consequent development of hyperglycemia. Diabetic patients, especially those who develop neuropathy, presented dysbiosis, with an increase in the proportion of pathogenic bacteria and a decrease in the butyrate-producing bacteria. Due to this dysbiosis, diabetic patients presented a weakness of the intestinal permeability barrier and high bacterial product translocation to the bloodstream, in parallel to a high circulating levels of pro-inflammatory cytokines such as TNF-α. In this context, we propose here that dysbiosis-induced increased systemic levels of bacterial products, like lipopolysaccharide (LPS), leads to an increase in the production of pro-inflammatory cytokines, including TNF-α, by Schwann cells and spinal cord of diabetics, being crucial for the development of neuropathy.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220197"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9174066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Giardia lamblia-infected preschoolers present growth delays independent of the assemblage A, B or E.","authors":"Maria Fantinatti,&nbsp;Tiara Cascais-Figueredo,&nbsp;Phelipe Austriaco-Teixeira,&nbsp;Filipe Anibal Carvalho-Costa,&nbsp;Alda Maria Da-Cruz","doi":"10.1590/0074-02760230043","DOIUrl":"https://doi.org/10.1590/0074-02760230043","url":null,"abstract":"<p><strong>Background: </strong>Intestinal parasite Giardia can affect children's physical development mainly stunting even in asymptomatic cases. The protozoa G. lamblia is divided into assemblages A-H. However, it is still unclear whether clinical manifestations and pathogenesis may vary according to the infecting assemblage.</p><p><strong>Objectives: </strong>To investigate whether G. lamblia assemblages influence differently the physical development of preschoolers from a community of Rio de Janeiro, Brazil.</p><p><strong>Methods: </strong>Anthropometric parameters were analysed from children attending a daycare centre and stool samples were obtained for the G. lamblia diagnosis. G. lamblia isolates from positive samples were genotyped. Data were analysed in order to verify whether there is a relationship between G. lamblia infection and the physical development of children according to the assemblage.</p><p><strong>Findings: </strong>Herein we demonstrated that although eutrophic, G. lamblia-infected daycare preschoolers from a low-income community presented growth delay compared to non-infected ones. This effect was observed for the three assemblages (A, B or E) found infecting humans.</p><p><strong>Main conclusion: </strong>G. lamblia causes growth delays on children independent of infecting assemblage (A, B or E).</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230043"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10065352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A short history of innate immunity.","authors":"Yuri Chaves Martins,&nbsp;Flávia Lima Ribeiro-Gomes,&nbsp;Cláudio Tadeu Daniel-Ribeiro","doi":"10.1590/0074-02760230023","DOIUrl":"https://doi.org/10.1590/0074-02760230023","url":null,"abstract":"<p><p>Innate immunity refers to the mechanisms responsible for the first line of defense against pathogens, cancer cells and toxins. The innate immune system is also responsible for the initial activation of the body's specific immune response (adaptive immunity). Innate immunity was studied and further developed in parallel with adaptive immunity beginning in the first half of the 19th century and has been gaining increasing importance to our understanding of health and disease. In the present overview, we describe the main findings and ideas that contributed to the development of innate immunity as a continually expanding branch of modern immunology. We start with the toxicological studies by Von Haller and Magendie, in the late 18th and early 19th centuries, and continue with the discoveries in invertebrate immunity that supported the discovery and characterization of lipopolysaccharide (LPS) and pattern recognition receptors that led to the development of the pattern recognition and danger theory.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230023"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10168657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9511296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stronger control-surveillance systems for vector-borne Chagas disease.","authors":"Rodrigo Gurgel-Gonçalves","doi":"10.1590/0074-02760210130chgsb","DOIUrl":"https://doi.org/10.1590/0074-02760210130chgsb","url":null,"abstract":"The multinational initiatives for the control/surveillance of Chagas launched by disease-endemic countries and the Pan American Health Organization-World Health Organization (PAHO-WHO) contributed to control house-infesting triatomine-bug populations and to reduce disease incidence. However, after 30 years, Chagas disease (CD) transmission persists the Americas. In their recent review, Rojas de Arias et al.(1) highlight the ‘practical impossibility’ of interrupting vector-borne Trypanosoma cruzi transmission, due to the zoonotic nature of most transmission cycles, with well over 100 vector species widely spread across the Americas. Then, Rojas de Arias et al.(1) emphasise the need for stronger surveillance systems to monitor and control CD. Here, I will (1) briefly discuss the prospects for interrupting vector-borne T. cruzi transmission and (2) provide an overview of some innovative approaches that, I believe, can play important roles in the development and operation of stronger control-surveillance systems for vector-borne CD. Disease control needs clear goals, and those goals often heavily depend on the natural history, risk factors, transmission routes and dynamics, pathogenesis, and treatment of the disease. Eradication, elimination, reduction of incidence, reduction of the number of severe cases, and reduction of fatality rates are all possible goals of disease control programs. (2) The most ambitious goal is disease eradication the complete elimination of an infection, with no new cases recorded in the absence of control measures. Eradication is practically impossible for zoonoses such as CD, whose etiological agent can be transmitted by 150+ vector species and infects a wide range of wild vertebrate hosts from the USA to Patagonia.(3) Therefore, the objective of a CD control program cannot be eradication, at least in the Americas. The main goal of the multinational initiatives against CD was the elimination of the strongly synanthropic, non-native populations of a few “primary” vectors mainly Triatoma infestans and Rhodnius prolixus.(4) Elimination of R. prolixus from Mexico and some countries of Central America was certified in 2011 by PAHO/WHO, but R. prolixus-infested houses were detected in rural sites in Mexico after certification.(5) Moreover, a new record of T. infestans in Mexico was recorded after 50 years.(6) In Peru, non-native T. infestans populations were not controlled in Arequipa (see references in Rojas de Arias et al.(1)). In addition, T. infestans residual foci have been detected in Brazil after certification.(7) Overall, these data indicate that elimination of non-native populations of “primary” vectors is feasible, but has many challenges. Elimination may not be the best goal for native vectors of CD.(8,9) There is a high species richness of Triatominae in the Americas.(3) Moreover, it is important to highlight that the response of some native species to vector control with insecticides was not the same as that observe","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"117 ","pages":"e210130chgsb"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9103131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal models and SARS-CoV-2-induced pulmonary and neurological injuries.","authors":"Marcelo Alves Pinto,&nbsp;Alexandre Dos Santos da Silva,&nbsp;Daniela Del Rosario Flores Rodrigues,&nbsp;Rodrigo Müller,&nbsp;Gentil Arthur Lins Bentes Mendonça de Vasconcelos,&nbsp;Patrícia Cristina da Costa Neves,&nbsp;Jaqueline Mendes de Oliveira,&nbsp;Renato Sergio Marchevsky","doi":"10.1590/0074-02760220239","DOIUrl":"https://doi.org/10.1590/0074-02760220239","url":null,"abstract":"<p><p>Laboratory animals are essential mainly for experiments aiming to study pathogenesis and evaluate antivirals and vaccines against emerging human infectious diseases. Preclinical studies of coronavirus disease 19 (COVID-19) pathogenesis have used several animal species as models: transgenic human ACE2 mice (K18 mice), inbred BALB/c or C57BL/6N mice, ferrets, minks, domestic cats and dogs, hamsters, and macaques. However, the choice of an animal model relies on several limitations. Besides the host susceptibility, the researcher's experience with animal model management and the correct interpretation of clinical and laboratory records are crucial to succeed in preclinical translational research. Here, we summarise pathological and clinical findings correlated with virological data and immunological changes observed from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) experimental infections using different well-established SARS-CoV-2 animal model species. This essay aims to critically evaluate the current state of animal model translation to clinical data, as described in the human SARS-CoV-2 infection.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"117 ","pages":"e220239"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10601766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An increased number of individuals of a potential host facilitates non-photic synchronisation in the haematophagous insect Triatoma infestans.","authors":"Veronica Sandra Valentinuzzi/,&nbsp;Luciana Beatriz Abrahan","doi":"10.1590/0074-02760220289","DOIUrl":"https://doi.org/10.1590/0074-02760220289","url":null,"abstract":"<p><strong>Background: </strong>Triatoma infestans (Kissing bug) is the main vector of the parasite causative of Chagas disease in Latin-America. This species shows clear activity rhythms easily synchronised to day-night cycles (photic cycle). The haematophagous nature of these insects lead us to think that they may temporally adapt to the particular activity rhythms of potential hosts (non-photic cycle). Our previous data showed that kissing bugs were weakly affected by the activity-inactivity rhythm of a single host.</p><p><strong>Objetive: </strong>To determine if by increasing the number of individuals of a potential host, T. infestans could increase the likelihood of synchronisation.</p><p><strong>Methods: </strong>Individual activity rhythms of experimental insects, maintained in constant darkness in light-tight cabinets, localised in a room with 24 rodents, were continuously monitored. Another insect group that served as control was maintained in the same conditions but in a room without rodents.</p><p><strong>Findings: </strong>Most of the experimental insects synchronised, expressing a 24 h period coincident with the activity-inactivity rhythms of the rodents, while the controls free ran with a period significantly longer than 24 h.</p><p><strong>Conclusion: </strong>Analogous to what happens with high vs low light intensity in photic synchronisers, a high number of rodents, in contrast to the previous one-rodent experiment, increased the potency of this non-photic zeitgeber.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220289"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10392893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10289474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and genome characterisation of SARS-CoV-2 P.6 lineage in dogs and cats living with Uruguayan COVID-19 patients.","authors":"Yanina Panzera,&nbsp;Santiago Mirazo,&nbsp;Mariana Baz,&nbsp;Claudia Techera,&nbsp;Sofía Grecco,&nbsp;Florencia Cancela,&nbsp;Eddie Fuques,&nbsp;Emma Condon,&nbsp;Lucía Calleros,&nbsp;Natalia Camilo,&nbsp;Andrea Fregossi,&nbsp;Inés Vaz,&nbsp;Paula Pessina,&nbsp;Nikita Deshpande,&nbsp;Ruben Pérez,&nbsp;Alejandro Benech","doi":"10.1590/0074-02760220177","DOIUrl":"https://doi.org/10.1590/0074-02760220177","url":null,"abstract":"<p><strong>Background: </strong>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in domestic animals have occurred from the beginning of the pandemic to the present time. Therefore, from the perspective of One Health, investigating this topic is of global scientific and public interest.</p><p><strong>Objectives: </strong>The present study aimed to determine the presence of SARS-CoV-2 in domestic animals whose owners had coronavirus disease 2019 (COVID-19).</p><p><strong>Methods: </strong>Nasopharyngeal and faecal samples were collected in Uruguay. Using quantitative polymerase chain reaction (qPCR), we analysed the presence of the SARS-CoV-2 genome. Complete genomes were obtained using ARTIC enrichment and Illumina sequencing. Sera samples were used for virus neutralisation assays.</p><p><strong>Findings: </strong>SARS-CoV-2 was detected in an asymptomatic dog and a cat. Viral genomes were identical and belonged to the P.6 Uruguayan SARS-CoV-2 lineage. Only antiserum from the infected cat contained neutralising antibodies against the ancestral SARS-CoV-2 strain and showed cross-reactivity against the Delta but not against the B.A.1 Omicron variant.</p><p><strong>Main conclusions: </strong>Domestic animals and the human SARS-CoV-2 P.6 variant comparison evidence a close relationship and gene flow between them. Different SARS-CoV-2 lineages infect dogs and cats, and no specific variants are adapted to domestic animals. This first record of SARS-CoV-2 in domestic animals from Uruguay supports regular surveillance of animals close to human hosts.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"117 ","pages":"e220177"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9182814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of killer immunoglobulin-like receptors genes on the recurrence rate of ocular toxoplasmosis in Brazil.","authors":"Daiana de Souza Perce-da-Silva,&nbsp;Thays Euzebio Joaquim,&nbsp;Ana Luisa Quintella do Couto Aleixo,&nbsp;Juliana Pessanha Rodrigues Motta,&nbsp;Josué da Costa Lima-Junior,&nbsp;Marcelo Ribeiro-Alves,&nbsp;Joseli de Oliveira-Ferreira,&nbsp;Luís Cristóvão de Moraes Sobrino Porto,&nbsp;Dalma Maria Banic,&nbsp;Maria Regina Reis Amendoeira","doi":"10.1590/0074-02760220203","DOIUrl":"https://doi.org/10.1590/0074-02760220203","url":null,"abstract":"<p><strong>Background: </strong>Recurrence is a hallmark of ocular toxoplasmosis (OT), and conditions that influence its occurrence remain a challenge. Natural killer cells (NK) are effectors cells whose primary is cytotoxic function against many parasites, including Toxoplasma gondii. Among the NK cell receptors, immunoglobulin-like receptors (KIR) deserve attention due to their high polymorphism.</p><p><strong>Objectives: </strong>This study aimed to analyse the influence of KIR gene polymorphism in the course of OT infection and its association with recurrences after an active episode.</p><p><strong>Methods: </strong>Ninety-six patients from the Ophthalmologic Clinic of the National Institute of Infectology Evandro Chagas were followed for up to five years. After DNA extraction, genotyping of the patients was performed by polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) utilising Luminex equipment for reading. During follow-up, 60.4% had a recurrence.</p><p><strong>Findings: </strong>We identified 25 KIR genotypes and found a higher frequency of genotype 1 (31.7%) with worldwide distribution. We note that the KIR2DL2 inhibitor gene and the gene activator KIR2DS2 were more frequent in patients without recurrence. Additionally, we observed that individuals who carry these genes progressed recurrence episodes slowly compared to individuals who do not carry these genes.</p><p><strong>Main conclusions: </strong>The KIR2DL2 and KIR2DS2 are associated as possible protection markers against ocular toxoplasmosis recurrence (OTR).</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220203"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10065411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9263159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography.","authors":"Hevandro de Souza Campos,&nbsp;Edimilson Domingos da Silva,&nbsp;Gerson Silva de Lima,&nbsp;Rafael de Oliveira Resende,&nbsp;Patricia Burth","doi":"10.1590/0074-02760220265","DOIUrl":"https://doi.org/10.1590/0074-02760220265","url":null,"abstract":"<p><strong>Background: </strong>Leptospirosis is an emerging zoonosis that affects humans and animals. Immunochromatography rapid test is widely used for early diagnosis of leptospirosis, but with low sensitivity and specificity.</p><p><strong>Objectives: </strong>To evaluate Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography.</p><p><strong>Methods: </strong>Insoluble fraction derived from the crude bacterial extract was obtained by serial centrifugation. The polypeptide profile was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Immune reactivity of this fraction was assessed by Western Blotting and lateral flow immunochromatography (LFI). It was tested 160 microagglutination test (MAT)-positive sera from patients in the acute phase, 100 MAT-negative sera from patients with acute febrile illness, and 45 patients with other infectious diseases.</p><p><strong>Findings: </strong>There was a predominance of low molecular mass-polypeptide bands, ranging from 2 to 37 kDa. The antibody reactivity of theses polypeptides was found to range from 13-50%, especially between 10 and 38 kDa. Among MAT-positive sera of patients with leptospirosis in the acute phase, 97% were also positive in LFI, indicating high sensitivity. Among MAT-negative sera, all were negative in LFI, indicating high specificity. Only 2% of cross-reactivity was detected.</p><p><strong>Conclusion: </strong>The insoluble fraction can be a valuable antigen source for development of point-of-care diagnosis test for leptospirosis.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220265"},"PeriodicalIF":2.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10187047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9866883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}