Simple and promising paper-based electrochemical platform for serological detection of American tegumentary leishmaniasis.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-02-12 eCollection Date: 2024-01-01 DOI:10.1590/0074-02760230149

Daniela E Barraza, Paula I Nanni, María E Bracamonte, Roberto E Chaile, Carla B Goy, Leonardo Acuña, Jorge D Marco, Rossana E Madrid

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引用次数: 0

Abstract

Background: American tegumentary leishmaniasis (ATL) is an endemic neglected tropical disease (NTD), its conventional treatment is toxic, slow, and invasive. Rapid diagnosis is crucial for the clinical management of suspected patients, so the development and use of low-cost, miniaturised and portable devices could be the key.

Objectives: This work aimed to develop a simple paper-based electrochemical platform for the serological detection of ATL.

Methods: Platform was fabricated in Whatman N°1 paper, contains a hydrophobic zone generated by wax printing, two pencil graphite electrodes, and uses specific crude extracts (CA) antigens for ATL immuno-determination. The platform performance was analysed by measuring the relative impedance change for different antigen-antibody combinations. Then, 10 serum human samples previously diagnosed by the gold standard (five positive ATL cases and five non-ATL cases) were evaluated.

Findings: The platform presented a linear response for the charge transfer resistance (ΔRct) and the interface reactance (ΔXc). Also, optimal working conditions were established (1/60 serum dilution and 180 µg/mL CA concentration). Then, the platform permits to distinguish between ATL and non-ATL (p < 0.05) human serum samples.

Main conclusions: Our platform could allow the diagnosis, management, and monitoring of leishmaniasis while being an extremely simple and environmentally friendly technology.

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用于美洲利什曼病血清学检测的纸质电化学平台操作简单、前景广阔。

背景：美洲利什曼病（ATL）是一种被忽视的热带地方病（NTD），其传统治疗方法具有毒性、缓慢和侵入性等特点。快速诊断对疑似患者的临床治疗至关重要，因此开发和使用低成本、小型化和便携式设备可能是关键：本研究旨在开发一种用于 ATL 血清学检测的简易纸基电化学平台：方法：平台采用 Whatman N°1 纸制作，包含一个由蜡印刷产生的疏水区和两个铅笔石墨电极，并使用特定的粗提取物（CA）抗原进行 ATL 免疫测定。通过测量不同抗原-抗体组合的相对阻抗变化，分析了该平台的性能。然后，对之前通过金标准诊断的 10 份人类血清样本（5 个 ATL 阳性病例和 5 个非 ATL 病例）进行了评估：该平台的电荷转移电阻（ΔRct）和界面电抗（ΔXc）呈线性响应。此外，还确定了最佳工作条件（1/60 血清稀释液和 180 µg/mL CA 浓度）。然后，该平台可以区分 ATL 和非 ATL（P < 0.05）人类血清样本：主要结论：我们的平台可用于利什曼病的诊断、管理和监测，是一项极其简单和环保的技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.