Ana Lua de Oliveira Vinhal, Max Roberto Batista de Araújo, Evandro Bento Rodrigues, Diogo Luiz de Carvalho Castro, Carine Rodrigues Pereira, Dircéia Aparecida Costa Custódio, Elaine Maria Seles Dorneles, Flávia Figueira Aburjaile, Bertram Brenig, Vasco Azevedo, Marcus Vinicius Canário Viana
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引用次数: 0
摘要
背景：金黄色棒状杆菌（Corynebacterium auriscanis）是一种经常从患有外耳道炎或皮炎的狗身上分离出来的细菌，也是一种通过狗咬人传播的人畜共患病原体。它被认为是一种机会性病原体，但对其致病机制研究甚少。比较基因组学可以确定毒力和生态位因素，有助于了解其生活方式：本项目旨在比较 C. auriscanis 的基因组，以确定与其毒力和生活方式有关的基因：方法：使用 Illumina HiSeq 2500 (Illumina, CA, USA) 对菌株 32 的基因组进行测序，并使用 Unicycler 进行组装。分析中还包括 GenBank 中提供的来自同一物种的另外两个非冗余基因组。对所有基因组进行了注释，并检查了分类、组装质量、移动元件、CRISPR-Cas 系统以及毒力基因和抗菌药耐药性基因。将这三个基因组中的毒力基因与通常与 C. auriscanis 分离的其他病原体中的毒力基因进行了比较：研究结果：该物种有 42 个毒力因子，可归类为生态位因子，因为原生病原体中没有真正的毒力因子。rbpA基因可赋予对利福平的基本抗性：主要结论：三个基因组中都没有真正的致病因子，这表明C. auriscanis的生活方式是一种机会性病原体。
First comparative genomics analysis of Corynebacterium auriscanis.
Background: Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle.
Objectives: The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle.
Methods: The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis.
Findings: The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin.
Main conclusions: The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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