Letters in Applied Microbiology最新文献_第3页

Integrating metagenomics and high-throughput screening for bioprospecting marine producers of polyhydroxyalkanoates. 整合宏基因组学和高通量筛选生物勘探海洋聚羟基烷酸酯生产者。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/lambio/ovaf096

Naicai Zhong, Yuan Chen, Wenfeng Pan, Hailin Meng, Kun Liang, Jun Lu, Yanlin Jiang, Chenyou Zhong, Muzhi Yang, Yinglei Zhai, Jinyi Zhong

{"title":"Integrating metagenomics and high-throughput screening for bioprospecting marine producers of polyhydroxyalkanoates.","authors":"Naicai Zhong, Yuan Chen, Wenfeng Pan, Hailin Meng, Kun Liang, Jun Lu, Yanlin Jiang, Chenyou Zhong, Muzhi Yang, Yinglei Zhai, Jinyi Zhong","doi":"10.1093/lambio/ovaf096","DOIUrl":"10.1093/lambio/ovaf096","url":null,"abstract":"With the escalating global demand for eco-friendly materials, polyhydroxyalkanoates (PHAs) have emerged as promising biodegradable alternatives to conventional plastics. This study is based on the systematic investigation of microbial community distribution and functional composition associated with PHA biosynthesis across diverse marine environments through metagenomic analysis. Leveraging environmental characteristics and PHA synthesis potential, we developed targeted culture media. We successfully isolated 102 PHA-producing bacterial strains from various marine habitats, including brackish water interfaces and deep-sea sediments. Gas chromatography quantification revealed a significant correlation (R² = 0.67) between Nile red fluorescence intensity and PHA content in metagenomically-predicted strains, effectively addressing false-positive issues in fluorescence-based screening. We subsequently established a high-throughput screening platform combining microplate technology with fluorometric quantification, identifying 10 elite strains for fermentation optimization and PHA characterization. Notably, strain R1-4-2 (identified as Halomonas olivaria) demonstrated exceptional performance with 2.59 g l-1 cell dry weight (CDW) and 52.89% PHA content (1.52 g l-1 CDW), representing both a novel microbial resource for sustainable PHA production and a potential chassis for synthetic biology applications. These findings provide critical scientific insights and technical frameworks for advancing industrial-scale production of biodegradable biopolymers.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative genomics and metabolic profiling of Rossellomorea sp. DL-A to understand the possible malathion degradation pathway. Rossellomorea sp. DL-A的整合基因组学和代谢分析以了解马拉硫磷可能的降解途径。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/lambio/ovaf103

Kiana Pillay, Gayatri Basapuram, Srimanti Duttagupta, Avishek Dutta

{"title":"Integrative genomics and metabolic profiling of Rossellomorea sp. DL-A to understand the possible malathion degradation pathway.","authors":"Kiana Pillay, Gayatri Basapuram, Srimanti Duttagupta, Avishek Dutta","doi":"10.1093/lambio/ovaf103","DOIUrl":"10.1093/lambio/ovaf103","url":null,"abstract":"Genomics and metabolic profiles were investigated to understand the malathion degradation pathways in Rossellomorea sp. DL-A strain, isolated from North Oconee River, GA, USA. The Rossellomorea sp. DL-A strain was able to grow in the presence of malathion. However, the growth pattern of the DL-A strain was impacted in the presence of malathion. The lag phase of DL-A's growth curve increased in the presence of malathion. Based on different conditions tested, Rossellomorea sp. DL-A had the highest degradation potential at pH 7 and 30°C. Genomic annotation revealed the presence of genes that were previously found in other malathion-degrading bacteria. Gas-Chromatography Mass Spectrometry (GC-MS) analyses also revealed a possible pathway of degradation of malathion by the DL-A strain. The results from untargeted GC-MS analyses and the presence of certain genes in the whole genome suggest that the products of malathion degradation are intermediates of different central carbon metabolism. This indicates that malathion can be used as a carbon source by Rossellomorea sp. DL-A. Further analyses revealed that the abundance of different intermediates of the malathion degradation pathway changed at different pH and temperatures, indicating that environmental conditions influence malathion degradation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell surface proteins of indigenous probiotics render antagonistic and protective effect on gut barrier integrity against extended-spectrum beta-lactamase (ESBL) Escherichia coli. 原生益生菌细胞表面蛋白对大肠杆菌扩展谱β -内酰胺酶（ESBL）具有拮抗和保护肠道屏障完整性的作用。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/lambio/ovaf110

Basavaprabhu Haranahalli Nataraj, Ramesh Chette, Rashmi Hogarehalli Mallappa

{"title":"Cell surface proteins of indigenous probiotics render antagonistic and protective effect on gut barrier integrity against extended-spectrum beta-lactamase (ESBL) Escherichia coli.","authors":"Basavaprabhu Haranahalli Nataraj, Ramesh Chette, Rashmi Hogarehalli Mallappa","doi":"10.1093/lambio/ovaf110","DOIUrl":"10.1093/lambio/ovaf110","url":null,"abstract":"Gut-mediated infections fostered by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are increasingly prevalent in clinical settings, posing a significant challenge to the efficacy of conventional antibiotic therapy. This underscores the urgent need to identify alternatives-to-antibiotics. In this study, we investigated the antagonistic potential of total surface proteins derived from probiotic strains viz. Limosilactobacillus fermentum LbS4 (MTCC 5954), L. fermentum Lf1 (MTCC 5689), Lactobacillus acidophilus NCFM, and Lacticaseibacillus rhamnosus GG (LGG), against clinical isolates of ESBL E. coli (9/234 and 23/208). Surface proteins were extracted using lithium chloride as chaotropic agent. Surface proteins exhibited strain-specific heterogeneity, as revealed by SDS-PAGE profiling. These proteins significantly (P < 0.05) reduced E. coli muco-adhesion through protective (hampering the bacterial adhesion), competitive (competition for adhesion sites), and displacement (dislodgement of adhered bacteria) mechanisms. While remaining non-toxic, surface proteins revealed anti-colonization efficacy against ESBL E. coli on HT-29 cell line and preserved epithelial integrity, as demonstrated by FITC-dextran transflux assays. In contrast, ESBL E. coli (109 CFU mL-1) compromised epithelial integrity by increasing FITC-dextran permeability, which was significantly (P < 0.05) mitigated by co-treatment with surface proteins. Overall, this study highlights the potential of probiotic-derived surface proteins as promising postbiotic candidates for countering ESBL E. coli colonization and preserving gut barrier integrity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and characterization of a cold-active, organic solvent-, and surfactant-tolerant cellulase from Pseudomonas fragi HsL3-1 isolated from Hengshui Lake sediments. 衡水湖沉积物中fragi假单胞菌hsl1 -1冷活性、耐溶剂和表面活性剂纤维素酶的纯化与表征

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/lambio/ovaf102

Chao Zhang, Jing Chen, Peixun Zhang, Jinnan Zong, Mingyu Geng, Xuying Yao, Jinxu Sun

{"title":"Purification and characterization of a cold-active, organic solvent-, and surfactant-tolerant cellulase from Pseudomonas fragi HsL3-1 isolated from Hengshui Lake sediments.","authors":"Chao Zhang, Jing Chen, Peixun Zhang, Jinnan Zong, Mingyu Geng, Xuying Yao, Jinxu Sun","doi":"10.1093/lambio/ovaf102","DOIUrl":"10.1093/lambio/ovaf102","url":null,"abstract":"Cold-active cellulases attract significant attention for their potential in energy-efficient bioprocesses under low-temperature conditions. In this study, a psychrotolerant bacterial strain, Pseudomonas fragi HsL3-1, was isolated from Hengshui Lake sediments and found to produce a novel endoglucanase, EG-22SJ. The enzyme demonstrated optimal activity at pH 5.0 and 25°C, retaining over 80% and 60% of peak activity at 15°C and 5°C, respectively, and exhibited exceptional tolerance to 20% organic solvents (e.g. n-hexane enhanced activity by 29.8%) and 1% surfactants (e.g. Tween 80). Kinetic analysis revealed high substrate affinity for CMC-Na with a Km of 0.583 mg·ml-1 and Vmax of 401 μmol·l-1·min-1. Activity was significantly activated by Ca²⁺ and Mg²⁺ but inhibited by Cu²⁺ and Hg²⁺. Culture optimization via response surface methodology increased cellulase production to 8.71 U·ml-1 under conditions of 15.24 g·l-1 CMC-Na, 20.54°C, pH 6.85, and 1.95% inoculation, yielding a 1.24-fold improvement. These integrated properties position EG-22SJ as a robust biocatalyst for sustainable low-temperature applications such as biofuel production, food processing, and detergent formulation, highlighting the potential of nonextreme environments for enzyme discovery.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete removal of COD from potato processing wastewater by co-cultivation of Haematococcus pluvialis and Gordonia terrae. 雨红球菌与地戈登菌共培养完全去除马铃薯加工废水中的COD。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-08-04 DOI: 10.1093/lambio/ovaf108

Dayang Gao, Jie Lv, Xiangying Xing, Qinglin Dong, Jiahui Tian, Xuan Zhou

引用次数: 0

Water supply from various sources has different effects on solid-state fermentation of Ganoderma lucidum. 不同水源对灵芝固态发酵的影响不同。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf095

Wei Yi, Qingmei Xiao, Mi Wei, Feitong Li, Longyu Wang, Jian He, Ang Ren, Ailiang Jiang, Ran Tao, Bangchao Zhong, Qin He

{"title":"Water supply from various sources has different effects on solid-state fermentation of Ganoderma lucidum.","authors":"Wei Yi, Qingmei Xiao, Mi Wei, Feitong Li, Longyu Wang, Jian He, Ang Ren, Ailiang Jiang, Ran Tao, Bangchao Zhong, Qin He","doi":"10.1093/lambio/ovaf095","DOIUrl":"10.1093/lambio/ovaf095","url":null,"abstract":"Water is essential in solid-state fermentation (SSF), but the impact of different water sources on SSF efficiency remains unclear. This study investigated the effects of different water sources on water-supply SSF of Ganoderma lucidum. The results showed that supplementation with normal saline led to the highest levels of laccase, CMCase, FPA activities, and biomass of G. lucidum (93.47, 140.34, 172.42 U/g, and 0.19 g/g, respectively), almost all significantly higher than those in the tap water group (83.57, 126.36, 167.17 U/g, and 0.18 g/g) and the deionized water group (77.25, 120.91, 145.50 U/g, and 0.16 g/g). Normal saline also significantly increased the capillary water content during SSF, which was 5.38% and 19.05% higher than that in the tap water and deionized water groups, respectively. Furthermore, the relaxation time of capillary water in the normal saline group decreased by 49.09%, a reduction that was higher than those observed in the tap water group (43.41%) and the deionized water group (29.56%). In conclusion, normal saline demonstrated distinct advantages in enhancing SSF efficiency, thus providing a scientific basis for the selection of water sources in the fermentation of G. lucidum and other microorganisms.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of inoculum composition on bacteria and bacteriophage UVC inactivation at 222 nm and 254 nm. 接种量组成对细菌和噬菌体在222 nm和254 nm灭活UVC的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf090

Katherine Ratliff, Michael Worth Calfee, Ahmed Abdel-Hady, Mariela Monge, Denise Aslett, Lukas Oudejans

{"title":"Impact of inoculum composition on bacteria and bacteriophage UVC inactivation at 222 nm and 254 nm.","authors":"Katherine Ratliff, Michael Worth Calfee, Ahmed Abdel-Hady, Mariela Monge, Denise Aslett, Lukas Oudejans","doi":"10.1093/lambio/ovaf090","DOIUrl":"10.1093/lambio/ovaf090","url":null,"abstract":"Using technologies that emit ultraviolet-C (UVC) radiation to inactivate pathogens has gained increasing interest as a strategy for reducing the risk of disease transmission, but it can be difficult to predict their performance in applied settings. Directly irradiating occupied spaces with emerging far-UVC has been proposed, as its shorter wavelength (200-230 nm) is reportedly less damaging to human eyes and skin than longer wavelengths. In this study, we conducted surface tests to evaluate the impact of different inoculum compositions (with and without soil loads) and conditions (wet vs. dried droplets) to determine how these factors affect the efficacy of UVC sources with peak emissions at 254 and 222 nm against five bacteria and two bacteriophages. The presence of a soil load reduced the efficacy of 222-nm UVC more than that at 254 nm, and both UVC sources were generally less effective against microbes in dried (vs. wet) inoculum. Given that pathogens will often be exposed to UVC in the presence of proteins, salts, and other constituents depending on how they are emitted, it is important to consider these challenges when quantifying efficacy.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Comparative analysis of the roles of catalases KatB and KatG in the physiological fitness and pathogenesis of fish pathogen Edwardsiella tarda. 校正：过氧化氢酶KatB和KatG在鱼类迟缓爱德华菌生理适应度和发病机制中的比较分析。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf098

引用次数: 0

Short-chain fatty acids and their role in modulating autoimmune responses in psoriasis: insights from recent microbiota research. 短链脂肪酸及其在调节银屑病自身免疫反应中的作用：来自最近微生物群研究的见解。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf091

Narges Fallahi, Mitra Rafiee, Seyedeh Sindokht Hosseini, Nasrin Sereshki, Gholamreza Anani Sarab, Nafiseh Erfanian

{"title":"Short-chain fatty acids and their role in modulating autoimmune responses in psoriasis: insights from recent microbiota research.","authors":"Narges Fallahi, Mitra Rafiee, Seyedeh Sindokht Hosseini, Nasrin Sereshki, Gholamreza Anani Sarab, Nafiseh Erfanian","doi":"10.1093/lambio/ovaf091","DOIUrl":"10.1093/lambio/ovaf091","url":null,"abstract":"Psoriasis, a chronic skin autoimmune disease characterized by abnormal immune responses, is influenced by genetic and environmental factors. Recent microbiota research has revealed that short-chain fatty acids (SCFAs), metabolites produced by gut microbiota, play a pivotal role in regulating immune function and inflammation. This review examines the current literature on the relationship between gut dysbiosis, SCFA production, and immune modulation in psoriasis, focusing on emerging evidence from microbiota and immunological studies. SCFAs have been shown to influence key immune pathways, including T-cell activation and cytokine production, which are critical in psoriasis pathogenesis. Reduced SCFA levels have been observed in psoriasis, highlighting the role of gut dysbiosis in disease progression. Understanding the gut-skin axis and the role of SCFAs offers novel insights into developing effective, safe, and accessible treatments for psoriasis. Restoring microbial balance and SCFA production may serve as a promising therapeutic approach for managing psoriasis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of heavy metal resistance and cross-/co-resistance to antibiotics in Pseudomonas aeruginosa. 铜绿假单胞菌重金属耐药及交叉/共耐药的分子机制。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf094

Bilel Hassen, Mohamed Salah Abbassi

{"title":"Molecular mechanisms of heavy metal resistance and cross-/co-resistance to antibiotics in Pseudomonas aeruginosa.","authors":"Bilel Hassen, Mohamed Salah Abbassi","doi":"10.1093/lambio/ovaf094","DOIUrl":"10.1093/lambio/ovaf094","url":null,"abstract":"Heavy metal pollution is a growing environmental and public health concern, particularly due to its impact on microbial communities. Pseudomonas aeruginosa, a highly adaptable bacterium, has developed resistance to heavy metals (HMs), which is closely linked to antibiotic resistance through shared genetic and regulatory pathways. This co-resistance poses significant challenges for environmental health and antimicrobial management. Additionally, microplastics act as carriers for HMs and antibiotics, creating a compounded pollution stressor that further influences bacterial resistance patterns. This review explores the molecular mechanisms by which P. aeruginosa resists heavy metal toxicity, focusing on key adaptive strategies such as efflux systems, biofilm formation, enzymatic detoxification, and genetic modifications. These mechanisms enhance bacterial survival in contaminated environments, allowing P. aeruginosa to persist and contribute to the spread of resistance genes. The interplay between HMs, antibiotics, and microplastics underscores the complexity of pollution-driven bacterial adaptation. Addressing these issues requires a multidisciplinary approach that integrates environmental pollution control and antimicrobial resistance management. Understanding how P. aeruginosa thrives under such stress conditions is crucial for developing effective strategies to mitigate the risks associated with heavy metal contamination, antibiotic resistance, and microplastic pollution in both natural and clinical ecosystems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0