Letters in Applied Microbiology最新文献_第3页

Optimization of the preparation of a spiny spore high-concentrated Aspergillus brasiliensis suspension. 刺孢子高浓度巴西曲霉悬浮液制备工艺优化。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae141

Federica Pisa, Mirella Gionta, Antonio Biasi, Maura Pizzuti, Jodi R Alt, Imma Zampaglione

{"title":"Optimization of the preparation of a spiny spore high-concentrated Aspergillus brasiliensis suspension.","authors":"Federica Pisa, Mirella Gionta, Antonio Biasi, Maura Pizzuti, Jodi R Alt, Imma Zampaglione","doi":"10.1093/lambio/ovae141","DOIUrl":"10.1093/lambio/ovae141","url":null,"abstract":"This work aimed to improve some steps of the existing guidelines of the European Standards to obtain an Aspergillus brasiliensis ATCC 16404 spore suspension with >75% spiny spores without mycelia and a concentration of at least 1.5 × 108 CFU ml-1. Several manufacturers' combinations of \"strain/medium\" were assessed in terms of yield of spiny spores. Criteria to establish success included (i) the growth over different times of incubation (4, 7, and 10 days); (ii) the performance of two different filtration devices (fritted filters and cell strainers); and (iii) the fungal spores susceptibility after 4 days of incubation versus 7 days of incubation against a peracetic acid-based disinfectant. The best combination was \"Microbiologics strain/Oxoid MEA\" with 88.33% of spiny spores already after 4 days, and no statistical differences (P < .05) in terms of growth in the three time points were highlighted (from 1.85 × 108 CFU ml-1 to 2.21 × 108 CFU ml-1). Cell strainers gave a spore suspension much more concentrated (3.31 times) compared to fritted filters. Furthermore, the 4-day-old spore suspension showed the same susceptibility to disinfection when compared to a suspension whose culture was incubated for 7 days.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of root-lesion nematode, Pratylenchus species, and their prevalence in New Zealand maize fields. 新西兰玉米根损线虫的分子特征及其流行状况。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae140

Nagarathnam Thiruchchelvan, Manjula Kularathna, Romy Moukarzel, Seona Casonato, Leo M Condron

{"title":"Molecular characterization of root-lesion nematode, Pratylenchus species, and their prevalence in New Zealand maize fields.","authors":"Nagarathnam Thiruchchelvan, Manjula Kularathna, Romy Moukarzel, Seona Casonato, Leo M Condron","doi":"10.1093/lambio/ovae140","DOIUrl":"10.1093/lambio/ovae140","url":null,"abstract":"Root-lesion nematodes (Pratylenchus spp.) are significant plant parasites, causing substantial crop damage worldwide. This study aimed to characterize Pratylenchus spp. in New Zealand maize fields using molecular techniques and map their prevalence. Soil sampling from 24 maize fields across the North and South Islands provided 381 composite samples. Root-lesion nematodes were extracted using the sieving-centrifugal-sugar flotation method and differentiated into five morphospecies. Molecular characterization involved direct partial sequencing of the D2/D3 28S rDNA, ITS rDNA, and COX1 mtDNA regions using Sanger technology from a single nematode. Five Pratylenchus species were identified: P. neglectus, P. crenatus, P. thornei, P. penetrans, and P. pratensis, confirmed by phylogenetic analysis. Prevalence mapping showed P. neglectus and P. crenatus in all sampled fields, while P. thornei, P. penetrans, and P. pratensis were more localized. This study is the first to report these Pratylenchus species on maize in New Zealand and provides the first partial sequences of the D2/D3, COX1, and ITS regions for these species on maize in New Zealand. The findings highlight the diversity of Pratylenchus populations in New Zealand maize fields and emphasize the need for region-specific management strategies to mitigate crop damage.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting. 利用移动实验室检测和定量废水中的 SARS-CoV-2

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae135

Shubham Mathur, Ikram Salah, Haneen Msarwa, Hillary A Craddock, Jacob Moran-Gilad

{"title":"Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting.","authors":"Shubham Mathur, Ikram Salah, Haneen Msarwa, Hillary A Craddock, Jacob Moran-Gilad","doi":"10.1093/lambio/ovae135","DOIUrl":"10.1093/lambio/ovae135","url":null,"abstract":"This study evaluated a point-of-care device for wastewater-based epidemiology (WBE) of SARS-CoV-2 circulation. WBE is a critical tool but faces challenges such as laboratory infrastructure, sample transport, skilled personnel, and time-consuming methods. Currently, a rapid, portable solution for wastewater surveillance is lacking. From June to September 2022, the performance of the GeneXpert instrument was compared with an established in-house method for detecting SARS-CoV-2 in 151 wastewater samples across laboratory, field, and mobile testing environments. In the laboratory setting, GeneXpert showed 100% agreement in detection, with 94.6% of positive samples displaying a viral load difference of ± 1 log compared to the in-house method. Field testing demonstrated 90% agreement, with 92.6% of GeneXpert-positive samples showing similar log differences. In mobile testing, GeneXpert detected 62.5% of samples, and testing faced technical issues. Overall, GeneXpert displayed a limit of detection of 12 cpml-1 with results available in under an hour, making it a promising tool for rapid and deployable wastewater testing. This requires further validation while addressing technical challenges related to field and mobile settings.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of multidrug-resistant ESBL-producing Enterobacteriaceae in livestock and in silico identification of probable catalytic domains in circulating ESBL variants. 畜禽中产生多重耐药ESBL的肠杆菌科细菌的出现及循环ESBL变体中可能催化结构域的计算机鉴定

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovaf003

Jai Sunder, Ayan Mukherjee, T Sujatha, Arun Kumar De, Debasis Bhattacharya, Samiran Bandyopadhyay, Aditya Paul, Indranil Samanta

{"title":"Occurrence of multidrug-resistant ESBL-producing Enterobacteriaceae in livestock and in silico identification of probable catalytic domains in circulating ESBL variants.","authors":"Jai Sunder, Ayan Mukherjee, T Sujatha, Arun Kumar De, Debasis Bhattacharya, Samiran Bandyopadhyay, Aditya Paul, Indranil Samanta","doi":"10.1093/lambio/ovaf003","DOIUrl":"10.1093/lambio/ovaf003","url":null,"abstract":"This study was conducted to detect the occurrence and phenotypic resistance pattern of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in livestock using docking-based analysis to reveal the classes of antibiotics against which ESBL-producers are active. Rectal swabs from healthy cattle (n = 100), goats (n = 88), and pigs (n = 66) were collected from backyard farms in Andaman and Nicobar Island (India). In total, 304 isolates comprising Escherichia coli (131), Salmonella (75), and Klebsiella pneumoniae (98) were recovered. The phenotypic resistance pattern showed that the highest resistance was found against erythromycin, followed by ampicillin/cloxacillin, ciprofloxacin, amoxicillin/clavulanic acid, and oxytetracycline. Screening of the isolates demonstrated maximum presence of blaTEM-Type, followed by blaCTX-M-Type, and blaSHV-Type. Sequence homology revealed the presence of CTX-M-15, SHV-228, SHV-40, and TEM-82. The possible interactions of CTX-M-15 with cephalosporins and SHV with carbapenems have been identified by docking analysis. The study revealed the occurrence of ESBL-producers with circulating emerging ESBL variants like SHV-40, along with the common variants with clinical relevance (CTX-M-15, TEM-82), highlighting the potential role of livestock as reservoirs for antimicrobial resistance genes. Identifying these ESBLs in livestock is crucial for understanding their contribution to the dissemination of antimicrobial resistant bacteria into the human food chain.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, validation, and implementation of multiplex digital PCR assays for simultaneous quantification of multiple targets. 设计，验证和实施多重数字PCR分析，同时定量多个目标。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae137

Jolinda de Korne-Elenbaas, Lea Caduff, Adrian Lison, Rachel McLeod, Melissa Pitton, Charles Gan, Timothy R Julian

{"title":"Design, validation, and implementation of multiplex digital PCR assays for simultaneous quantification of multiple targets.","authors":"Jolinda de Korne-Elenbaas, Lea Caduff, Adrian Lison, Rachel McLeod, Melissa Pitton, Charles Gan, Timothy R Julian","doi":"10.1093/lambio/ovae137","DOIUrl":"10.1093/lambio/ovae137","url":null,"abstract":"Quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) are applied for quantifying molecular targets in disease diagnostics, pathogen detection, and ecological monitoring. Uptake of dPCR is increasing due to its higher quantification accuracy relative to qPCR, which stems from its independence from standard curves and its increased resistance to PCR inhibitors. Throughput can be increased through multiplexing, which allows simultaneous quantification of multiple targets. However, multiplexing with dPCR faces unique challenges relative to qPCR. Here, we describe the three-phase development process of non-competing multiplex dPCR assays using target-specific fluorescently labeled hydrolysis probes. We highlight common challenges encountered, along with recommended solutions. Phase 1: In silico assay design; target-specific primers and probes are selected or designed, potential issues with primer and probe interactions are identified, and fluorophores and quenchers are chosen based on dPCR instrumentation. Phase 2: Wet-lab validation; assays are benchmarked using positive controls. Insufficient performance leads to assay redesign, as needed. Phase 3: Assay implementation; assay specificity and sensitivity are validated on relevant sample matrices. Finally, we provide recommendations on the future design and standardization of multiplexed dPCR assays, highlighting the need for better in silico predictions of assay performance, standardizing positive controls, and automating partition classification systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thank you to reviewers. 感谢审稿人。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovaf001

引用次数: 0

Antibacterial, antibiofilm, and gene expression assessment of ajwain (Trachyspermum ammi) essential oil on drug-resistant gastrointestinal pathogens and its combination effect with ampicillin. 阿月浑子（Trachyspermum ammi）精油对耐药性胃肠道病原体的抗菌、抗生物膜和基因表达评估及其与氨苄西林的联合作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae138

Mahdi Asghari Ozma, Niloofar Fallahi Alileh, Amin Abbasi, Sina Mahdavi, Manouchehr Fadaee, Javad Nezhadi, Masoud Asghari Ozma, Mohammad Asgharzadeh, Hossein Samadi Kafil

{"title":"Antibacterial, antibiofilm, and gene expression assessment of ajwain (Trachyspermum ammi) essential oil on drug-resistant gastrointestinal pathogens and its combination effect with ampicillin.","authors":"Mahdi Asghari Ozma, Niloofar Fallahi Alileh, Amin Abbasi, Sina Mahdavi, Manouchehr Fadaee, Javad Nezhadi, Masoud Asghari Ozma, Mohammad Asgharzadeh, Hossein Samadi Kafil","doi":"10.1093/lambio/ovae138","DOIUrl":"10.1093/lambio/ovae138","url":null,"abstract":"Essential oils are natural substances used as therapeutic agents and food preservatives to inhibit harmful microorganisms. This study aimed to assess the synergistic effect of Trachyspermum ammi essential oil and ampicillin on antibiotic-resistant gastrointestinal pathogens, including Escherichia coli, Enterococcus faecalis, Shigella flexneri, and Salmonella serotype Typhimurium. Using gas chromatography-mass spectrometry (GC-MS), the main components of T. ammi essential oil were identified as thymol, gamma terpenes, and cymene. The antibacterial and antibiofilm properties were evaluated by minimum inhibitory concentration (MIC), disk diffusion, and microtiter plate methods, revealing MIC values of 2, 1, 4, and 4 mg ml-1 for E. coli, E. faecalis, S. flexneri, and S. Typhimurium, respectively, and inhibition zones between 10 and 14 mm. Pathogens were examined for their biofilm-related virulence genes, including aggR, esp, icsA, and fliC, using real-time polymerase chain reaction (RT-PCR) in E. coli, E. faecalis, S. flexneri, and S. Typhimurium, respectively. The methyl thiazole tetrazolium (MTT) assay was used to evaluate the essential oil's effect on the viability of human embryonic kidney 293 (HEK293) cells, which showed cell viability of over 80%. The combination of T. ammi oil and ampicillin demonstrated a synergistic effect, and biofilm formation was inhibited. E. faecalis exhibited the greatest sensitivity, while S. flexneri exhibited the lowest sensitivity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of Amikacin-loaded carbopol hydrogel for topical treatment of Mycobacterium marinum skin infections. 载阿米卡霉素卡波醇水凝胶局部治疗海洋分枝杆菌皮肤感染的研制与评价。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae134

Praise Mary Francis, Vivek Vinod, V Anil Kumar, C Gopi Mohan, Raja Biswas

{"title":"Development and evaluation of Amikacin-loaded carbopol hydrogel for topical treatment of Mycobacterium marinum skin infections.","authors":"Praise Mary Francis, Vivek Vinod, V Anil Kumar, C Gopi Mohan, Raja Biswas","doi":"10.1093/lambio/ovae134","DOIUrl":"10.1093/lambio/ovae134","url":null,"abstract":"Mycobacterium marinum is a slow growing Non-Tuberculosis Mycobacteria known to cause skin and subcutaneous tissue infections known as \"fish tank granuloma\" in humans. Treatment of M. marinum skin infections can last for several months or even years. Intravenous or intramuscular administration of Amikacin, a bactericidal inhibitor, in a multidrug regimen is a good therapeutic option in treating M. marinum infections. No topical hydrogels are currently available to treat M. marinum skin infections. We developed a 2.5% amikacin-loaded carbopol hydrogel containing the permeation enhancers 1% Tween-80 and 1% PEG (AMK-CPTP) for topical application to treat M. marinum skin infections. A molecular docking study of carbopol-Amikacin interaction predicted a stable structure and was confirmed with rheological characterization. The developed gel was found to be non-hemolytic, cytocompatible, and non-irritant. Sustained release of Amikacin from the AMK-CPTP hydrogel was observed, with a release of 60% Amikacin after 48 h. The antibacterial activity and skin permeability of amikacin released from AMK-CPTP were assessed using both in vitro and ex vivo assays. Overall. AMK-CPTP demonstrated anti-microbial activity against M. marinum with a 35% increase in skin permeation on the addition of Tween-80 and PEG. AMK-CPTP could be used to treat M. marinum skin infections.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic antimicrobial effects of thiamine dilauryl sulphate combined with the heartwood extract of Pterocarpus marsupium and antifungal mechanism in their combination. 硫酸硫胺二auryl与有袋翼果心材提取物的协同抑菌作用及其协同抑菌机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae139

Moe Manbo Inoue, Hideki Kurotaki, Naoko Mizuhara, Akira Ogita, Kazuyori Matsumoto, Ken-Ichi Fujita

{"title":"Synergistic antimicrobial effects of thiamine dilauryl sulphate combined with the heartwood extract of Pterocarpus marsupium and antifungal mechanism in their combination.","authors":"Moe Manbo Inoue, Hideki Kurotaki, Naoko Mizuhara, Akira Ogita, Kazuyori Matsumoto, Ken-Ichi Fujita","doi":"10.1093/lambio/ovae139","DOIUrl":"10.1093/lambio/ovae139","url":null,"abstract":"Thiamine dilauryl sulphate (TDS) exerts a bacteriostatic effect against Gram-positive bacteria and is used as a food additive. The heartwood extract of the Indian kino tree (Pterocarpus marsupium), a medicinal tree native to India, shows weak antimicrobial effects against bacteria and superficial dermatophyte-infective fungi. Herein, we report that the combination of TDS and P. marsupium extract exerts an additive or synergistic antimicrobial effect against various food spoilage microorganisms. Among the 13 species tested, this combination showed synergistic antimicrobial effects against Bacillus subtilis, Lactococcus lactis, Lactobacillus plantarum, and Candida albicans in an agar dilution method. We also found that the combination promoted the production of reactive oxygen species against a model yeast Saccharomyces cerevisiae using a fluorescnt 2',7'-dichlorofluorescein diacetate. Furthermore, the combination restricted drug efflux from yeast cells via efflux pumps in an efflux assay using a fluorescent rhodamine 6 G. These results suggest that the antifungal effect of TDS is improved in combination with P. marsupium extract via oxidative stress induction and drug efflux restriction. The combination of TDS and P. marsupium extract has a low environmental impact and toxicity when used in foods; therefore, it is also expected to be used in food and industrial products in the future.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of rice and edible starch compound ratios, starch type, and rice nutrients on Burkholderia gladioli pathovar cocovenenans growth and bongkrekic acid production in wet rice noodles and starch products. 大米与食用淀粉复合比例、淀粉种类和大米营养成分对湿米线和淀粉制品中角兰伯克氏菌生长和产酸的影响

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae124

Jingmin Huang, Jianfei Huang, Xiaoqing Liu, Leshi Li, Zeyu Lin, Xiaole Liu, Xintian Lai, Lirong Kang, Jingru Liao, Jing Chen

{"title":"The effect of rice and edible starch compound ratios, starch type, and rice nutrients on Burkholderia gladioli pathovar cocovenenans growth and bongkrekic acid production in wet rice noodles and starch products.","authors":"Jingmin Huang, Jianfei Huang, Xiaoqing Liu, Leshi Li, Zeyu Lin, Xiaole Liu, Xintian Lai, Lirong Kang, Jingru Liao, Jing Chen","doi":"10.1093/lambio/ovae124","DOIUrl":"10.1093/lambio/ovae124","url":null,"abstract":"Bongkrekic acid (BA) toxin, produced by Burkholderia gladioli pathovar cocovenenans bacteria, has been implicated in foodborne illness outbreaks. BA poisoning is associated with rice noodle consumption; hence, this study investigated B. cocovenenans growth and BA production in wet rice noodles comprising varying starch ratios, starch types, rice nutrients, and saccharides. Noodles with 100% rice flour had BA concentrations 47.7 times higher than that of 100% starch samples (30.53 ± 1.08 mg∙kg-1 vs 0.65 ± 0.03 mg∙kg-1). Moreover, protein powder and glucose significantly promoted BA production (P < 0.05). Corn starch with 120 μl∙100 ml-1 rice oil had a BA concentration of 0.23 ± 0.01 mg∙kg-1, which was 31% lower than those without rice oil. Acid-treated starch had a BA concentration 7.24 times higher than that of other natural starch and no BA was detected in acetate starch. Of the carbohydrates tested, only hexacarbon sugars stimulated BA production. Overall, the degree of carbohydrate hydrolysis critically influenced BA formation. Increasing the use of edible starch and reducing the amount of polysaccharides hydrolyzed into monosaccharides during production will mitigate the risk of B. cocovenenans-related food poisoning in rice noodles and starch products. Rice oil serves as a possible additive to hinder BA production.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0