Letters in Applied Microbiology最新文献_第3页

Lactiplantibacillus plantarum P10 alleviates hyperuricemia-induced inflammation in mice by modulating gut microbiota and histidine metabolism. 植物乳杆菌P10通过调节肠道菌群和组氨酸代谢减轻小鼠高尿酸血症诱导的炎症。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-09-04 DOI: 10.1093/lambio/ovag068

Lixin Song, Binbin Tang, Min He, Jie Zhang, Yan Bao, Yingbo Xie, Min Zhang, Mengting Pang, Fei Hou, Rong Jin

{"title":"Lactiplantibacillus plantarum P10 alleviates hyperuricemia-induced inflammation in mice by modulating gut microbiota and histidine metabolism.","authors":"Lixin Song, Binbin Tang, Min He, Jie Zhang, Yan Bao, Yingbo Xie, Min Zhang, Mengting Pang, Fei Hou, Rong Jin","doi":"10.1093/lambio/ovag068","DOIUrl":"10.1093/lambio/ovag068","url":null,"abstract":"Hyperuricaemia is a common chronic metabolic disorder characterized by urate crystal deposition and systemic inflammation. Lactic acid bacteria have been demonstrated to exert probiotic effects against hyperuricaemia. This study investigated the probiotic effects and potential mechanisms of Lactiplantibacillus plantarum P10 in a murine hyperuricaemic model. The results showed that L. plantarum P10 significantly reduced serum uric acid levels, inhibited xanthine oxidase activity, and decreased the activity of hepatic function markers, including aspartate aminotransferase and alanine aminotransferase, as well as the levels of renal function markers, including creatinine and blood urea nitrogen. Notably, these functional improvements were accompanied by alleviated histopathological inflammatory lesions in the liver and kidney, together with reduced levels of the pro-inflammatory cytokines interleukin-1β and tumour necrosis factor-α. Further investigation revealed that L. plantarum P10 modulated renal expression of the urate transporters ABCG2 and GLUT9, promoting uric acid excretion. Moreover, multi-omics analyses indicated that L. plantarum P10 enriched the relative abundance of Lactobacillus johnsonii and Limosilactobacillus reuteri, ameliorated gut dysbiosis, and altered the histidine pathway. In conclusion, L. plantarum P10 alleviates urate overload-induced renal injury and inflammation by modulating gut microbiota and histidine metabolism, offering potent urate-lowering, anti-inflammatory, and renoprotective benefits for hyperuricaemia intervention.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A natural product ginsenoside C-K with antimicrobial activity against Streptococcus pneumoniae. 具有抗菌肺炎链球菌活性的天然产物人参皂苷C-K。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-09-04 DOI: 10.1093/lambio/ovag083

Haibo Wang, Lifang Xu, Xuehui Sheng, Liqiang Yu, Qianqian Wang, Xianbin Song, Jiangang Zhu

{"title":"A natural product ginsenoside C-K with antimicrobial activity against Streptococcus pneumoniae.","authors":"Haibo Wang, Lifang Xu, Xuehui Sheng, Liqiang Yu, Qianqian Wang, Xianbin Song, Jiangang Zhu","doi":"10.1093/lambio/ovag083","DOIUrl":"10.1093/lambio/ovag083","url":null,"abstract":"Streptococcus pneumoniae is a major cause of bacteremia and sepsis, and rising antimicrobial resistance necessitates new therapies. This study aimed to screen ginsenosides with antipneumococcal activity and to evaluate antibacterial effects of candidate compound. Among nine ginsenosides tested in vitro, only ginsenoside C-K exhibited significant antibacterial activity with a minimum inhibitory concentration (MIC) of 4-8 µg·mL-1. Further investigation demonstrated that ginsenoside C-K exerted rapid bactericidal effects and effectively inhibited both the formation and stability of biofilms. In addition, it disrupted bacterial membrane integrity and induced the accumulation of reactive oxygen species (ROS). Moreover, ginsenoside C-K treatment of S. pneumoniae-infected cells was associated with the restoration of mitochondrial membrane potential and reduced expression of pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β. In vivo, treatment with ginsenoside C-K significantly improved the survival rate of infected hosts to 60%-70%. In summary, ginsenoside C-K exhibits potent antimicrobial activity both in vitro and in vivo, combining antibacterial, antibiofilm, and antiinflammatory effects. This study provides new experimental evidence supporting the application of ginsenoside-derived natural products in the treatment of pneumococcal infections and offers valuable insight for the development of novel antimicrobial lead compounds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genetic determinants of quinolone resistance in Escherichia coli at the human-swine interface of an agroecological farm. 某农业生态农场人猪界面大肠杆菌喹诺酮类药物耐药性的表型和遗传决定因素

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag062

Guillermina Sánchez-Martínez, Rosa I Santamaría, Elsa Tamayo-Legorreta, Celia Alpuche-Aranda, Humberto Barrios-Camacho, Ulises Garza-Ramos, Jesús Silva-Sánchez, Víctor González

{"title":"Phenotypic and genetic determinants of quinolone resistance in Escherichia coli at the human-swine interface of an agroecological farm.","authors":"Guillermina Sánchez-Martínez, Rosa I Santamaría, Elsa Tamayo-Legorreta, Celia Alpuche-Aranda, Humberto Barrios-Camacho, Ulises Garza-Ramos, Jesús Silva-Sánchez, Víctor González","doi":"10.1093/lambio/ovag062","DOIUrl":"10.1093/lambio/ovag062","url":null,"abstract":"Quinolone-resistant Escherichia coli are increasing in humans and livestock; however, their distribution and underlying mechanisms in shared environments remain incompletely characterized. We analyzed quinolone resistance in E. coli isolates from swine (n = 100) and humans (n = 65) associated with an agroecological farm in Morelos, Mexico. Antimicrobial susceptibility testing was performed for quinolones [nalidixic acid (NAL) and ciprofloxacin (CIP)], and the minimum inhibitory concentrations were determined. Phylogenetic grouping and genotypic analyses targeting gyrA mutations and plasmid-mediated quinolone resistance (PMQR) genes were conducted. High frequencies of NAL and moderate CIP resistance were observed in both swine (NAL, 79%; CIP, 23%) and human (NAL, 65%; CIP, 15%) isolates. The predominant phenotype was NAL-resistant/CIP-susceptible, particularly among commensals of phylogroups A and B1. High-level resistance to both antimicrobials was associated with double mutations in gyrA (S83L + D87N), whereas PMQR determinants, including qnr variants and aac(6')-Ib-cr, were widely distributed and linked to lower-level resistance. These findings indicate that quinolone resistance is shaped by the combined contribution of chromosomal mutations and plasmid-mediated mechanisms. The presence of PMQR determinants in isolates with reduced susceptibility highlights their potential role in the early stages of resistance development, emphasizing the urgent need for integrated surveillance of antimicrobial resistance in agricultural and community settings.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial glucosamine production: current strategies, process bottlenecks, and future perspectives. 微生物葡萄糖胺生产：当前策略、工艺瓶颈和未来展望。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag058

Sourav Ranjan Parida, Subhransu Sekhar Behera, Lopamudra Ray

{"title":"Microbial glucosamine production: current strategies, process bottlenecks, and future perspectives.","authors":"Sourav Ranjan Parida, Subhransu Sekhar Behera, Lopamudra Ray","doi":"10.1093/lambio/ovag058","DOIUrl":"10.1093/lambio/ovag058","url":null,"abstract":"Glucosamine and its derivatives have been extensively used in the nutraceutical, cosmetic, food and pharmaceutical industries. Commercial glucosamine production traditionally relies on the acid or enzymatic hydrolysis of crustacean shells, particularly those of shrimp and crabs. However, these processes are often associated with high production costs, environmental concerns, extensive chemical usage, and the generation of hazardous waste. In recent years, microbial production of glucosamine has emerged as a sustainable and environmentally friendly alternative, offering advantages such as milder processing conditions, reduced chemical inputs, and improved process scalability. Microbial approaches include direct glucosamine biosynthesis by native microorganisms, bioconversion of chitin-rich substrates by chitinolytic microbes, and metabolic engineering of recombinant strains for enhanced production. This review explores microbial approaches for glucosamine production, including direct biosynthesis, chitin bioconversion, and recombinant microbial systems. Key challenges related to substrate utilization, process scalability, strain stability, and product recovery are critically evaluated, along with emerging solutions involving metabolic engineering and process optimisation. Additionally, strategies to overcome such challenges such as CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)-based gene editing, optimization of culture and fermentation conditions etc. have been discussed. This review emphasizes the fact that while microbial production of glucosamine offers clear environmental and economic advantages, their large-scale feasibility depends on addressing these challenges through integrated approaches.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative proteomic profiling of Streptococcus agalactiae recovered from the kidneys of diabetic mice. 糖尿病小鼠肾脏中无乳链球菌的比较蛋白质组学分析。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag060

João Matheus Sobral Pena, Lucas Lima de Oliveira, Yuri Pereira Souza, Carina Ferreira Barnabé, Gabriela da Silva Santos, Nathália Curty de Andrade, Dário Eluan Kalume, Prescilla Emy Nagao

{"title":"Comparative proteomic profiling of Streptococcus agalactiae recovered from the kidneys of diabetic mice.","authors":"João Matheus Sobral Pena, Lucas Lima de Oliveira, Yuri Pereira Souza, Carina Ferreira Barnabé, Gabriela da Silva Santos, Nathália Curty de Andrade, Dário Eluan Kalume, Prescilla Emy Nagao","doi":"10.1093/lambio/ovag060","DOIUrl":"10.1093/lambio/ovag060","url":null,"abstract":"Streptococcus agalactiae is a significant opportunistic pathogen causing severe infections, including urinary tract infections and pyelonephritis in diabetic individuals. To explore the molecular mechanisms behind this susceptibility, we used a streptozotocin-induced diabetic murine model to evaluate S. agalactiae renal infection and performed the first comparative proteomic analysis of S. agalactiae COH1 isolates recovered from the kidneys of diabetic and non-diabetic mice. Diabetic mice exhibited significantly higher bacterial loads and more severe renal histopathological damage. Comparative proteomic profiling identified 2751 proteins, with 82 significantly upregulated and 91 downregulated proteins in S. agalactiae COH1 isolate recovered from the kidneys of diabetic animals. Upregulated virulence factors included serine-rich repeat 2, type VII secretion, BibA, C5a peptidase, and PI-1. Functional analysis revealed a metabolic shift toward increased glycolysis, PTS system activity, and translation machinery, suggesting that the hyperglycemia promoted bacterial proliferation and energy production. Conversely, proteins related to proteolysis were downregulated. Our findings demonstrated that hyperglycemia induced a metabolic reprogramming and enhanced the expression of virulence-associated proteins in S. agalactiae. These adaptations contributed to increased colonization and tissue damage in diabetic kidney, providing potential molecular targets for future therapeutic interventions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactiplantibacillus plantarum NCHBL-004 improves intestinal motility and modulates serotonergic gene expression in a loperamide-induced constipation model. 植物乳杆菌NCHBL-004在洛哌丁胺诱导便秘模型中改善肠道蠕动并调节血清素能基因表达。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag063

Wan-Gyu Kim, Tae-Sung Lee, Chang-Jung Kim, Yeong-Jun Kim, In-Su Seo, So-Yeon Ahn, Ji-Yeong Kim, Sang-Eun Jung, Geun-Woo Kim, Eun-Jung Song, Ah-Ra Jang, Hong-Bum Koh, Jong-Hwan Park

{"title":"Lactiplantibacillus plantarum NCHBL-004 improves intestinal motility and modulates serotonergic gene expression in a loperamide-induced constipation model.","authors":"Wan-Gyu Kim, Tae-Sung Lee, Chang-Jung Kim, Yeong-Jun Kim, In-Su Seo, So-Yeon Ahn, Ji-Yeong Kim, Sang-Eun Jung, Geun-Woo Kim, Eun-Jung Song, Ah-Ra Jang, Hong-Bum Koh, Jong-Hwan Park","doi":"10.1093/lambio/ovag063","DOIUrl":"10.1093/lambio/ovag063","url":null,"abstract":"Slow-transit constipation (STC) is characterized by reduced colonic motility, impaired smooth muscle function, and altered mucosal signaling. Among these pathways, mucosal serotonin (5-HT), mainly produced by enterochromaffin cells, contributes to intestinal motility regulation. In a loperamide-induced constipation model in male C57BL/6 J mice, we evaluated the effects of Lactiplantibacillus plantarum NCHBL-004 on bowel function and associated molecular changes. Compared with loperamide-treated mice, live NCHBL-004 at 1 × 10⁹ CFU improved fecal output and gastrointestinal transit and partially preserved fecal water content, whereas the lower live dose and heat-killed preparation showed no significant effects. These functional changes were accompanied by preservation of colonic muscularis thickness and increased expression of smooth muscle-associated markers, including Acta2 and Myh11. NCHBL-004 also modulated mucosal serotonergic gene expression, as indicated by increased Tph1, which encodes a key enzyme for 5-HT synthesis, and decreased Slc6a4, which encodes the serotonin transporter involved in 5-HT reuptake. Overall, these findings suggest that live NCHBL-004 improves intestinal motility in loperamide-induced constipation, with associated changes in colonic smooth muscle phenotype and mucosal serotonergic regulation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-read rRNA operon sequencing reveals bacterial community divergence between traditional and industrial soybean fermentations. 长读rRNA操纵子测序揭示了传统和工业大豆发酵之间的细菌群落差异。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag064

Sung-Youn Kim, Yejin Kim, Dae-Jung Kim, Han Sub Chang

{"title":"Long-read rRNA operon sequencing reveals bacterial community divergence between traditional and industrial soybean fermentations.","authors":"Sung-Youn Kim, Yejin Kim, Dae-Jung Kim, Han Sub Chang","doi":"10.1093/lambio/ovag064","DOIUrl":"10.1093/lambio/ovag064","url":null,"abstract":"Traditional and industrial soybean fermentations harbor distinct microbial communities that influence product quality and safety. In this study, long-read rRNA operon sequencing was applied to investigate the bacterial communities of traditional and commercial doenjang and cheonggukjang. The 16S-23S rRNA operon (∼4.2 kb) was amplified and sequenced using the Oxford Nanopore MinION platform to obtain high-resolution taxonomic profiles. Traditional doenjang and cheonggukjang exhibited greater microbial diversity than commercial products, as revealed by alpha and beta diversity analyses. Bacillus species, particularly Bacillus velezensis and Bacillus subtilis, were dominant across all samples, while Loigolactobacillus coryniformis, Caldifermentibacillus hisashii, and Tetragenococcus halophilus were more abundant in traditionally fermented samples. These genera are associated with proteolysis, amino acid metabolism, and flavor compound formation during fermentation. Overall, these findings provide insights into the microbial ecology of fermented soybean foods and suggest that non-starter lactic acid bacteria and thermophilic species may contribute to the unique sensory characteristics of traditionally fermented doenjang.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional dermatological formulation with sophorolipids from Starmerella bombicola and copaiba essential oil: antimicrobial, antiviral, and antioxidant properties. 多功能皮肤学配方，含有从蚕豆草和可帕依巴精油中提取的槐脂：抗菌，抗病毒和抗氧化特性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag067

Tainã de Oliveira Ferreira, Briani Gisele Bigotto, Débora Dahmer, Niumaique Gonçalves da Silva, Sabrina Aparecida Balsarini Marqueti, Yara Dos Santos Vieira Dias, Gerson Nakazato, Renata Katsuko Takayama Kobayashi, Sueli Fumie Yamada Ogatta, Audrey Alessandra S G Lonni, Maria Antonia P Colabone Celligoi

{"title":"Multifunctional dermatological formulation with sophorolipids from Starmerella bombicola and copaiba essential oil: antimicrobial, antiviral, and antioxidant properties.","authors":"Tainã de Oliveira Ferreira, Briani Gisele Bigotto, Débora Dahmer, Niumaique Gonçalves da Silva, Sabrina Aparecida Balsarini Marqueti, Yara Dos Santos Vieira Dias, Gerson Nakazato, Renata Katsuko Takayama Kobayashi, Sueli Fumie Yamada Ogatta, Audrey Alessandra S G Lonni, Maria Antonia P Colabone Celligoi","doi":"10.1093/lambio/ovag067","DOIUrl":"10.1093/lambio/ovag067","url":null,"abstract":"The combination of sophorolipids and copaiba essential oil in a multifunctional facial formulation aimed at the treatment of conditions such as acne and herpetic folliculitis was evaluated. Sophorolipids produced by Starmerella bombicola reached 38.37 g/l, predominantly in the lactonic form. Their antimicrobial activity, alone and combined with copaiba oil, was evaluated against Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. The combination reduced the minimum inhibitory concentration against C. acnes from 62.50 to 31.25 μg/ml, demonstrating an additive effect. Antiviral assays showed strong anti-HSV-1 activity, with 95.58% inhibition in the virucidal test and 63.99% in the antiviral assay. Three dermocosmetic formulations were developed, and the optimized formulation achieved 91.45% inhibition of C. acnes and 26.17% antioxidant activity, confirming its multifunctional potential. The application of a formulation combining sophorolipids and copaiba oil shows promising potential for the treatment of skin conditions, demonstrating significant antimicrobial activity against C. acnes as well as adequate physicochemical stability.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148679160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic mining of Bacillus safensis and Enterococcus lactis from food sources. 食物源中萨氏芽孢杆菌和乳酸肠球菌的基因组挖掘。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag061

Daniel Jesuwenu Ajose, Ifeoma Irene Adetoyinbo, Christ-Donald Kaptchouang Tchatchouang

{"title":"Genomic mining of Bacillus safensis and Enterococcus lactis from food sources.","authors":"Daniel Jesuwenu Ajose, Ifeoma Irene Adetoyinbo, Christ-Donald Kaptchouang Tchatchouang","doi":"10.1093/lambio/ovag061","DOIUrl":"10.1093/lambio/ovag061","url":null,"abstract":"The growing complexity of food-safety systems and the increasing emergence of multidrug-resistant (MDR) foodborne pathogens demonstrate the importance of enhanced genomic surveillance. This study employed whole genome sequencing (WGS) to characterise the genomes of Bacillus safensis NWU MK_WT, Enterococcus lactis ENT7_CNKT_NWU, and ENT3_CNKT_NWU, isolated from food sources. Phenotypic antibiotic susceptibility testing revealed that all strains displayed MDR phenotypes, with resistance to erythromycin, ampicillin, and meropenem. Genome assemblies ranged from 2.6 to 3.7 Mb, exhibiting high completeness (100%) and diverse functional gene profiles. Furthermore, antibiotic resistance genes (ARGs), including vanT and aac(6'), mediating antibiotic inactivation, efflux, and target modification, were identified. Virulence factors, including adhesion, invasion, and biofilm formation, were detected across genomes, indicating pathogenic potential. Mobile genetic element profiling revealed the presence of insertion sequences, plasmids, and an intact prophage in B. safensis NWU MK_WT, demonstrating genomic plasticity and the potential for horizontal gene transfer (HGT). Phylogenomic comparison showed close relatedness between the isolates and strains from Asia, suggesting possible transboundary movement of genetic material. These findings highlight the growing relevance of WGS for monitoring opportunistic foodborne bacteria that harbour and disseminate resistance and virulence determinants, provide foundational data for improving food safety surveillance, and support antimicrobial resistance (AMR) mitigation strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148471438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using double-layer agar and checkerboard assays to detect phage cocktail synergy with selected antimicrobials in Pseudomonas aeruginosa. 采用双层琼脂法和棋盘法检测铜绿假单胞菌中噬菌体鸡尾酒与选定抗菌剂的协同作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag065

Umut Yilmaz, Özlem Erkoç Güleryüz, Mehmet Ünlü, Gülhan Vardar Ünlü

{"title":"Using double-layer agar and checkerboard assays to detect phage cocktail synergy with selected antimicrobials in Pseudomonas aeruginosa.","authors":"Umut Yilmaz, Özlem Erkoç Güleryüz, Mehmet Ünlü, Gülhan Vardar Ünlü","doi":"10.1093/lambio/ovag065","DOIUrl":"10.1093/lambio/ovag065","url":null,"abstract":"The increasing prevalence of multidrug-resistant (MDR) Pseudomonas aeruginosa highlights the need for alternative therapeutic strategies. This study evaluated the potential of a commercial bacteriophage cocktail combined with antimicrobial agents and compared two practical methods for screening phage-antimicrobial synergy. A lytic bacteriophage isolated from thepyophage (PYO) phage cocktail was characterized by host range, size, pH, and thermal stability, and lytic activity against the reference strain PAO1. Synergy was assessed with nine antimicrobial agents, including conventional antibiotics, efflux pump inhibitors (EPIs), and essential oil compounds, using agar-based plaque assays and broth microdilution checkerboard assays. Plaque diameter and plaque number were evaluated at sub-MIC concentrations, whereas synergistic interactions were determined by the fractional inhibitory concentration index (FICI). Statistical significance was analyzed using one-way ANOVA followed by Dunnett's multiple comparison test, and the association between plaque parameters and FICI values was explored using Spearman's rank correlation. The isolated phage exhibited a burst size of approximately 259 PFU per infected cell and remained stable over a broad pH and temperature range, with significant reductions under highly acidic, highly alkaline, and high-temperature conditions. In agar-based assays, colistin, meropenem, and cinnamaldehyde significantly increased plaque diameter and/or plaque number (P <0.05-0.001), depending on the bacterial strain tested. Checkerboard analysis identified the same four combinations as synergistic (FICI ≤0.5). Spearman's rank correlation indicated a relationship between plaque parameters and FICI values. Overall, both methods identified the same synergistic combinations, suggesting that agar-based plaque assays may serve as a practical preliminary screening tool for phage-antimicrobial combinations, although further validation is required.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148604629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0