Letters in Applied Microbiology最新文献_第3页

Native bradyrhizobia for soybean: genetic and functional diversity in Heihe soils, a major production zone of Heilongjiang, China. 大豆原生缓生根瘤菌：黑龙江省主要产区黑河土壤的遗传和功能多样性

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag026

Ke Li, Keyu Chen, Haoran Hao, Kaiwen Zhang, Brigitte Brunel, Wenshan Zhou, Junjie Zhang

{"title":"Native bradyrhizobia for soybean: genetic and functional diversity in Heihe soils, a major production zone of Heilongjiang, China.","authors":"Ke Li, Keyu Chen, Haoran Hao, Kaiwen Zhang, Brigitte Brunel, Wenshan Zhou, Junjie Zhang","doi":"10.1093/lambio/ovag026","DOIUrl":"10.1093/lambio/ovag026","url":null,"abstract":"Soybean (Glycine max) forms symbiotic nitrogen fixation with rhizobia, and compatible, efficient rhizobia in soils are vital for its sustainable production. This study analyzed the distribution and traits of native soybean-associated rhizobia in soils from Heihe, Heilongjiang Province, to identify strains with high symbiotic nitrogen fixation efficiency and environmental adaptability, and select candidates for local microbial inoculants to boost sustainable soybean production. Seventy-four rhizobial isolates were obtained from three Heilongjiang sampling sites using a local soybean variety, and characterized genetically and symbiotically. PCR-RFLP of IGS DNA grouped them into 9 genotypes. Multilocus sequence analysis (16S rRNA, recA, atpD, gyrB genes) placed representative strains into three known species (Bradyrhizobium japonicum, B. diazoefficiens, B. ottawaense) and an uncharacterized Bradyrhizobium group (64%, dominant). Phylogeny of nodC/nifH markers showed affiliation with symbiovar glycinearum. All strains nodulated soybean with symbiotic efficiency (67-88%); about half enhanced plant biomass. Three strains (DG28, GCZ12, SH16) showing superior symbiotic efficiency. Representative strains had varied tolerance to alkalinity, high temperature, and PEG-induced drought. Strain SH16 combined high efficiency and stress tolerance. These results provide promising candidates for local soybean inoculant development.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of rifaximin and rifampicin resistance across Clostridioides difficile ribotypes: a systematic review and meta-analysis. 艰难梭菌核型对利福昔明和利福平耐药性的评估：一项系统综述和荟萃分析。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag018

Nasser Alkhushaym, Rawan Alabbad, Yousef Saeed Alqarni, Naif Almalki, Reem Khader Alanazi, Abdullah Alhifany

{"title":"Assessment of rifaximin and rifampicin resistance across Clostridioides difficile ribotypes: a systematic review and meta-analysis.","authors":"Nasser Alkhushaym, Rawan Alabbad, Yousef Saeed Alqarni, Naif Almalki, Reem Khader Alanazi, Abdullah Alhifany","doi":"10.1093/lambio/ovag018","DOIUrl":"10.1093/lambio/ovag018","url":null,"abstract":"Clostridioides difficile is an opportunistic pathogen of the gastrointestinal tract that can cause illnesses ranging from diarrhea to pseudomembranous colitis. Recurrent Clostridioides difficile infection remains a major clinical challenge, with substantial relapse rates after standard antibiotic therapy. Emerging evidence suggests that rifaximin can be used after the conventional therapy to reduce risk of the recurrence. However, rifaximin resistance in Clostridioides difficile remains the primary concern. The aim of this systematic review and meta-analysis is to estimate the risk of rifaximin resistance in Clostridioides difficile infection and among different ribotypes. The search included PubMed, Scopus, Web of Science, and Cochrane Library for studies reporting rifaximin resistance in Clostridioides difficile isolates. After systematically screening 731 records from all databases and excluding 664 studies, a total of 67 studies were included in the meta-analysis. The findings of the meta-analaysis indicated a resistance rate of 15.1% (95% CI, 12.0%-18.9%) for rifaximin and/or rifampicin and 12.8% (95% CI, 8.8%-18.2%) for rifaximin alone. Ribotype-specific analysis revealed high rifaximin resistance in RT017 (72.3%), RT027 (47.0%), and RT018 (20.9%), while RT012, RT002, RT112, and RT014/020 demonstrated low resistance. The study finding indicate that rifaximin/rifamycin resistance in Clostridioides difficile is concerning and not randomly distributed but is more frequently associated with certain ribotypes.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Staphylococcus aureus extracellular vesicles: navigating challenges, embracing prospects. 金黄色葡萄球菌细胞外囊泡研究进展：挑战与展望。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag009

Chunyan Fu, Zeqi Li, Yanwen Sun, Shaohua Lin, Xuejing Yang, Xiaojuan Li

{"title":"Advances in Staphylococcus aureus extracellular vesicles: navigating challenges, embracing prospects.","authors":"Chunyan Fu, Zeqi Li, Yanwen Sun, Shaohua Lin, Xuejing Yang, Xiaojuan Li","doi":"10.1093/lambio/ovag009","DOIUrl":"10.1093/lambio/ovag009","url":null,"abstract":"Staphylococcus aureus has emerged as a globally widespread pathogen, and its growing antibiotic resistance poses severe challenges to human health. With the advancing research on bacterial extracellular vesicles (bEVs), S. aureus EVs, have been found to act as pathogenic factors, exacerbating the pathophysiology of S. aureus infections. This review summarizes current research on S. aureus EVs, outlining their roles in promoting virulence and contributing to antibiotic resistance, which provides insights into the pathogenic mechanisms and the emergence of resistance in S. aureus. We also summarize certain natural compounds that exhibit disruptive effects on S. aureus EVs, which may offer new strategies for treating S. aureus infections. Although S. aureus EVs aggravate disease progression, their unique properties can be harnessed through bioengineering techniques. This review further highlights the potential applications of S. aureus EVs in vaccine development, drug delivery, and innovative anti-tumor or antiviral therapies. However, the rapid emergence of new technologies and the discovery of non-vesicular nanoparticles have cast doubt on previously established findings. Therefore, this review also addresses the following questions: How should we interpret these existing discoveries? And how should we advance future research related to S. aureus EVs?","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of methicillin-resistant Staphylococcus aureus and multidrug resistance among food handlers in a hospital food and nutrition unit in Rio de Janeiro, Brazil. 巴西里约热内卢一家医院食品和营养部门食品处理人员中耐甲氧西林金黄色葡萄球菌和多药耐药性的流行情况

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag021

Rosevane de Oliveira Cunha, Douglas Guedes, Elaine de Oliveira Pinto, Simone Alves, Aline Dos Santos Garcia-Gomes

{"title":"Prevalence of methicillin-resistant Staphylococcus aureus and multidrug resistance among food handlers in a hospital food and nutrition unit in Rio de Janeiro, Brazil.","authors":"Rosevane de Oliveira Cunha, Douglas Guedes, Elaine de Oliveira Pinto, Simone Alves, Aline Dos Santos Garcia-Gomes","doi":"10.1093/lambio/ovag021","DOIUrl":"10.1093/lambio/ovag021","url":null,"abstract":"Foodborne diseases are a major public health concern, and food handlers play a key role in the transmission of pathogenic and antimicrobial-resistant microorganisms. This study evaluated the occurrence and antimicrobial resistance profiles of Staphylococcus aureus among 50 food handlers working in the food and nutrition unit of a public hospital in Rio de Janeiro, Brazil. Samples from hands and nasal cavities were analyzed using culture-based methods and identified by MALDI-TOF mass spectrometry. Staphylococcus aureus was detected in 12% of hand samples and 22% of nasal swabs. High resistance rates were observed for penicillin, erythromycin, and ampicillin. Methicillin-resistant S. aureus represented 29% of hand isolates and 47% of nasal isolates, and 26% were multidrug resistant. These findings indicate that food handlers may act as reservoirs and transmission sources of antimicrobial resistance in hospital food service settings, highlighting the need for continuous monitoring, strict hygiene practices, and antimicrobial stewardship programs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First evidence of SO2-releasing bags controlling fungal growth, aflatoxins, and cyclopiazonic acid contamination in unshelled peanuts. 释放二氧化硫袋控制去壳花生真菌生长、黄曲霉毒素和环吡唑酸污染的首个证据。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag029

Carla Cervini, Vaibhav Verma, Carol Verheecke-Vaessen, Angel Medina, Naresh Magan

{"title":"First evidence of SO2-releasing bags controlling fungal growth, aflatoxins, and cyclopiazonic acid contamination in unshelled peanuts.","authors":"Carla Cervini, Vaibhav Verma, Carol Verheecke-Vaessen, Angel Medina, Naresh Magan","doi":"10.1093/lambio/ovag029","DOIUrl":"10.1093/lambio/ovag029","url":null,"abstract":"Peanuts (also known as groundnuts; Arachis hypogaea L.) are a globally significant cash crop but are highly susceptible to fungal contamination, particularly by Aspergillus section Flavi, which can contaminate the product with mycotoxins, including aflatoxins. This contamination poses serious food safety concerns, especially in low- and middle-income countries, limiting access to international markets. This study assessed the efficacy of slow-release SO2-bags compared to plastic bags (control) in reducing fungal growth and mycotoxin contamination in stored unshelled peanuts. Naturally contaminated and Aspergillus flavus-inoculated peanuts were stored at water activity (aw) levels of 0.85 and 0.95 for 15 days at 25°C. Fungal populations were monitored, and aflatoxins and cyclopiazonic acid were analysed using LC-MS/MS qTRAP. SO2-releasing bags completely inhibited fungal growth in naturally contaminated peanuts and significantly reduced A. flavus population with effectiveness dependent on water activity. Additionally, SO2-releasing bags suppressed mycotoxin production. This study demonstrates, for the first time, the effectiveness of SO2-releasing bags in preventing fungal spoilage and mycotoxin contamination in postharvest peanuts, offering a promising solution for enhancing peanut safety and quality.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into membrane disruption and oxidative stress in Gram-positive and Gram-negative bacteria: role of sodium benzoate and phenoxyethanol. 对革兰氏阳性和革兰氏阴性细菌的膜破坏和氧化应激的见解：苯甲酸钠和苯氧乙醇的作用。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag022

Ishani Sharma, S M Rose, Madhu Lata, Somnath Das, Nagaraja Is Acharya, Maheshwara Naik, Samiran Mahapatra, Sharmistha Sinha

{"title":"Insights into membrane disruption and oxidative stress in Gram-positive and Gram-negative bacteria: role of sodium benzoate and phenoxyethanol.","authors":"Ishani Sharma, S M Rose, Madhu Lata, Somnath Das, Nagaraja Is Acharya, Maheshwara Naik, Samiran Mahapatra, Sharmistha Sinha","doi":"10.1093/lambio/ovag022","DOIUrl":"10.1093/lambio/ovag022","url":null,"abstract":"This study investigates the antibacterial effects of two widely used preservatives in foods, pharmaceuticals, and personal care products, sodium benzoate and phenoxyethanol, on Gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Gram-negative bacteria Pseudomonas aeruginosa (MTCC 1688). Despite their widespread usage, the exact antibacterial mechanisms of these preservatives are not yet fully understood. Our study demonstrated that the presence of SB and POE lead to extensive cellular rupture, membrane damage, and intracellular content leakage in bacteria. The results were validated through biochemical assays and visualized by transmission electron microscopy. The key findings from this study reveals that P. aeruginosa when treated with SB leads to cell shrinkage, whereas POE treatment leads to cellular bulging, under varied concentrations. Further analysis demonstrates that both compounds induce intracellular aldehyde accumulation leading to an increase in oxidative stress. This increase in oxidative stress can cause protein cross-linking, DNA and membrane destabilization, leading to cellular disruption. In summary, this study sheds light on the mechanisms behind the antibacterial effects and preservative's efficacy of these well-established compounds, offering valuable information for their ongoing use and potential enhancement in microbial control strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities. 使用元条形码检测在基于培养的海洋沉积物细菌群落分析中快速开始的培养偏差。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag020

Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park

{"title":"Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities.","authors":"Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park","doi":"10.1093/lambio/ovag020","DOIUrl":"10.1093/lambio/ovag020","url":null,"abstract":"When cultivation-based microbiology is used to isolate strains from environmental samples, the cultured populations may not represent ecologically relevant taxa in the source community. To address this, we employed pre-cultivation metabarcoding to establish a baseline community profile and detect cultivation bias. Using time-resolved cultivation of marine sediment bacteria, we demonstrated the need for initial community characterization. Sediment-derived microbiomes were cultured in Marine Broth 2216 and analyzed using 16S rRNA gene metabarcoding at 0, 6, 12, 18, and 24 h. A rapid 10-fold reduction in alpha diversity was observed within the 6 h (from 1029 amplicon sequence variants to 34-106), with the genus Vibrio reaching near-complete dominance (>95%) from 18 to 24 h, while environmentally dominant taxa such as Acinetobacter were quickly excluded. This dramatic shift illustrates that, without baseline characterization, cultivation-induced artifacts cannot be clearly distinguished from ecologically meaningful patterns. Fast-growing generalists can quickly outcompete ecologically significant taxa, distorting isolation outcomes and hindering the recovery of functionally important microorganisms. We show that metabarcoding at 0 h can identify cultivation biases, help interpret isolation results, and suggest targeted strategies for recovering ecologically relevant taxa. This integrated approach facilitates more accurate recovery and analysis of functionally significant microbial diversity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecology and antimicrobial resistance of Campylobacter in wildlife: insights into specialist and generalist lineages and zoonotic potential. 野生动物弯曲杆菌的生态学和抗菌素耐药性：对专业和通用谱系和人畜共患潜力的见解。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag031

Manel Gharbi, Mohamed Salah Abbassi

{"title":"Ecology and antimicrobial resistance of Campylobacter in wildlife: insights into specialist and generalist lineages and zoonotic potential.","authors":"Manel Gharbi, Mohamed Salah Abbassi","doi":"10.1093/lambio/ovag031","DOIUrl":"10.1093/lambio/ovag031","url":null,"abstract":"Wildlife is a critical reservoir of Campylobacter species, particularly C. jejuni and C. coli, carrying diverse genetic lineages, virulence factors, and antimicrobial resistance (AMR) genes. Birds, especially migratory and synanthropic species, are the primary carriers, though mammals, reptiles, and other vertebrates also contribute to maintenance and dissemination. Wildlife-associated strains include both host-specific lineages and generalist clonal complexes (e.g. ST21, ST45, and ST828) capable of crossing wildlife, livestock, humans, and environmental interfaces, reflecting high zoonotic potential. Virulence factors, including motility, adhesion and invasion proteins (CadF, CiaB), and cytolethal distending toxin (CDT), facilitate colonization and survival, while efflux pumps and stress-response genes enhance persistence under antibiotic pressure. AMR is widespread, with resistance to fluoroquinolones, macrolides, tetracyclines, and multidrug phenotypes, driven by anthropogenic contamination, environmental reservoirs, and horizontal gene transfer. Key resistance determinants include gyrA mutations, tet(O), erm(B), cmeABC efflux pumps, and β-lactamases. Despite advances, knowledge gaps remain, particularly for non-avian hosts, environmental reservoirs, and resistance mechanisms. A One Health approach integrating microbiology, genomics, ecology, and epidemiology is essential to map transmission pathways, monitor emerging resistance, and guide interventions to reduce the public health impact of zoonotic and antibiotic-resistant Campylobacter.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bactericidal and anti-virulence effects of Melaleuca alternifolia essential oil against foodborne pathogens: in vitro and milk matrix assay. 互花千层精油对食源性病原菌的杀菌和抗毒作用：体外和乳基质试验。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag030

Lucília Rocha Magna, Milena Matesco Carreteiro, Gabriel José Couto, Luana de Carvalho, Matheus Henrique Nogueira, Mariana Lima Braga, André Ricardo Peron Dos Santos, Márcia Cristina Furlaneto, Regiane da Silva Gonzalez, Luciana Furlaneto Maia

{"title":"Bactericidal and anti-virulence effects of Melaleuca alternifolia essential oil against foodborne pathogens: in vitro and milk matrix assay.","authors":"Lucília Rocha Magna, Milena Matesco Carreteiro, Gabriel José Couto, Luana de Carvalho, Matheus Henrique Nogueira, Mariana Lima Braga, André Ricardo Peron Dos Santos, Márcia Cristina Furlaneto, Regiane da Silva Gonzalez, Luciana Furlaneto Maia","doi":"10.1093/lambio/ovag030","DOIUrl":"10.1093/lambio/ovag030","url":null,"abstract":"The increasing demand for natural antimicrobials has driven interest in plant-derived compounds for food safety applications. This study evaluated the antimicrobial activity of tea tree essential oil (Melaleuca alternifolia, TTO) against Escherichia coli and Salmonella spp., combining in vitro and in situ assays. Minimum inhibitory concentrations (MICs) ranged from 3.12 to 6.25 µg ml-1, and the minimum bactericidal concentrations were identical to the MICs, in media broth. Time-kill assays showed significant reductions in viable cells at 1× MIC, with complete inactivation of S. Enteritidis and S. Typhimurium within 24 h. Treatment with TTO resulted in significant changes in zeta potential and increased leakage of nucleic acids and proteins, indicating membrane destabilization. At sub-inhibitory concentrations, TTO reduced swimming motility and biofilm formation in a strain-dependent manner. In sterilized milk, MIC values were identical to those obtained in Brain Heart Infusion (BHI) for all Salmonella strains and for E. coli P2421, while E. coli ATCC 25922 exhibited a higher MIC (6.25 µg ml-1). Checkerboard assays with potassium sorbate showed an additive interaction for S. enteritidis (FICI 1.0), indifferent interactions for most strains, and antagonism for S. Typhi, resulting in a reduction in sorbate MIC from 5.0 to 2.5 mg ml-1 in selected strains.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary supplementation with chicken-derived L. rhamnosus CIQ249 protects broilers against enteropathogens by regulating intestinal homeostasis. 饲粮中添加鸡源性鼠李糖乳杆菌CIQ249可通过调节肠道内稳态来保护肉鸡免受肠道病原体的侵袭。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag024

Yimei Liu, Jiayi Xiang, Han Yu, Renjie Xu, Moxuan Mao, Jiacun Liu, Jiangfei Zhou, Jing Wang, Yigang Xu, Yuan Li

{"title":"Dietary supplementation with chicken-derived L. rhamnosus CIQ249 protects broilers against enteropathogens by regulating intestinal homeostasis.","authors":"Yimei Liu, Jiayi Xiang, Han Yu, Renjie Xu, Moxuan Mao, Jiacun Liu, Jiangfei Zhou, Jing Wang, Yigang Xu, Yuan Li","doi":"10.1093/lambio/ovag024","DOIUrl":"10.1093/lambio/ovag024","url":null,"abstract":"Lacticaseibacillus rhamnosus is commonly used to manage gastrointestinal infections in children, yet its efficacy as a probiotic feed additive for poultry against enteropathogenic bacteria remains insufficiently explored. In this study, a strain L. rhamnosus (CIQ249) was isolated from chicken intestine and evaluated for its ability to enhance intestinal homeostasis and confer resistance against enteropathogens using chicks as animal model. CIQ249 exhibited strong potential for intestinal colonization and high tolerance to gastrointestinal stress, including acid, bile, and osmotic pressure. In vitro, CIQ249 showed effective inhibitory activity against pathogenic bacteria as determined by agar well diffusion assay. During a one-month feeding trial, dietary supplementation with CIQ249 significantly improved the growth performance of broilers. In subsequent experimental challenges, oral gavage administration of CIQ249 conferred protection against E. coli O78 and S. Typhimurium. Furthermore, CIQ249 modulated intestinal immune response by increasing levels of IL-4, IL-10, IFN-γ, and sIgA, while suppressing production of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α). It also upregulated expression of tight junction proteins (ZO-1 and Claudin-1), alleviating gut damage induced by pathogenic infections. These findings indicate that CIQ249 is a promising probiotic feed additive that protects chickens from enteropathogenic bacteria by reinforcing intestinal epithelial barrier and enhancing immune homeostasis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0