Impact of inoculum composition on bacteria and bacteriophage UVC inactivation at 222 nm and 254 nm.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Katherine Ratliff, Michael Worth Calfee, Ahmed Abdel-Hady, Mariela Monge, Denise Aslett, Lukas Oudejans
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引用次数: 0

Abstract

Using technologies that emit ultraviolet-C (UVC) radiation to inactivate pathogens has gained increasing interest as a strategy for reducing the risk of disease transmission, but it can be difficult to predict their performance in applied settings. Directly irradiating occupied spaces with emerging far-UVC has been proposed, as its shorter wavelength (200-230 nm) is reportedly less damaging to human eyes and skin than longer wavelengths. In this study, we conducted surface tests to evaluate the impact of different inoculum compositions (with and without soil loads) and conditions (wet vs. dried droplets) to determine how these factors affect efficacy of UVC sources with peak emissions at 254 nm and 222 nm against five bacteria and two bacteriophages. The presence of a soil load reduced 222 nm UVC efficacy more than 254 nm, and both UVC sources were generally less effective against microbes in dried (vs. wet) inoculum. Given that pathogens will often be exposed to UVC in the presence of proteins, salts, and other constituents depending on how they are emitted, it is important to consider these challenges when quantifying efficacy.

接种量组成对细菌和噬菌体在222 nm和254 nm灭活UVC的影响。
使用发射紫外线c (UVC)辐射的技术来灭活病原体作为一种降低疾病传播风险的策略已经引起了越来越多的兴趣,但是很难预测它们在应用环境中的性能。据报道,远紫外线波长较短(200-230纳米)对人眼和皮肤的伤害比波长较长的要小,因此有人建议用远紫外线直接照射占据的空间。在这项研究中,我们进行了表面测试,以评估不同接种成分(有和没有土壤负荷)和条件(湿滴和干滴)的影响,以确定这些因素如何影响峰值发射在254 nm和222 nm的UVC源对五种细菌和两种噬菌体的效果。土壤负荷的存在使222 nm的UVC效力比254 nm的降低,并且两种UVC源在干燥(与湿)接种物中对微生物的作用通常较低。鉴于病原体经常会暴露在有蛋白质、盐和其他成分存在的UVC中,这取决于它们是如何释放的,因此在量化功效时考虑这些挑战是很重要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Letters in Applied Microbiology
Letters in Applied Microbiology 工程技术-生物工程与应用微生物
CiteScore
4.40
自引率
4.20%
发文量
225
审稿时长
3.3 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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