Letters in Applied Microbiology最新文献

Are foliar extracts of mycorrhizal Hymenaea martiana toxic to human peripheral blood mononuclear cells? 菌根膜菌叶提取物对人外周血单核细胞有毒性吗？

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf075

Brena Coutinho Muniz, Carlos André Ribeiro Costa, Caio Bezerra Barreto, Jefferlone Lopes da Silva Filho, Maria Rafaele Oliveira Bezerra da Silva, Silvana de Fátima Ferreira da Silva, Daniela de Araújo Viana Marques, Qiang-Sheng Wu, Fábio Sérgio Barbosa da Silva

{"title":"Are foliar extracts of mycorrhizal Hymenaea martiana toxic to human peripheral blood mononuclear cells?","authors":"Brena Coutinho Muniz, Carlos André Ribeiro Costa, Caio Bezerra Barreto, Jefferlone Lopes da Silva Filho, Maria Rafaele Oliveira Bezerra da Silva, Silvana de Fátima Ferreira da Silva, Daniela de Araújo Viana Marques, Qiang-Sheng Wu, Fábio Sérgio Barbosa da Silva","doi":"10.1093/lambio/ovaf075","DOIUrl":"10.1093/lambio/ovaf075","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are known to enhance the accumulation of bioactive compounds with medicinal properties in plants. However, the potential cytotoxic effects of extracts from mycorrhizal plants on peripheral blood mononuclear cells (PBMC) remain unexplored. Therefore, this study aimed to verify the cytotoxic potential of foliar extract from Hymenaea martiana Hayne seedlings, either associated or not associated with AMF on PBMC. A greenhouse experiment was conducted with two treatments: a control group (without AMF) and a group inoculated with Acaulospora longula Spain and N.C. Schenck. After 148 days, leaves were collected to prepare aqueous extracts, and cytotoxicity of the extracts was assessed using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Additionally, the antioxidant potential and the content of soluble carbohydrates, flavonoids, flavonols, flavonones, dihydroflavonols, and saponins were assessed. Hymenaea martiana seedlings associated with A. longula exhibited a more than 50% increase in the accumulation of phenolic compounds compared to the control. However, no toxicity was detected for PBMC under any of the conditions evaluated. This study provides the first evidence of the effect of mycorrhizal plant extracts on human blood cells, highlighting their potential safety for medicinal and cosmetic applications.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atoxigenic isolates of Aspergillus flavus effectively reduce cyclopiazonic acid in a sorghum-based matrix under simulated abiotic stress conditions. 模拟非生物胁迫条件下，黄曲霉产氧分离株在高粱基质中有效还原环吡唑酸

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf072

Vanshika Sharma, Carla Cervini, Carol Verheecke-Vaessen, Ranajit Bandyopadhyay, Angel Medina, Alejandro Ortega-Beltran, Naresh Magan

{"title":"Atoxigenic isolates of Aspergillus flavus effectively reduce cyclopiazonic acid in a sorghum-based matrix under simulated abiotic stress conditions.","authors":"Vanshika Sharma, Carla Cervini, Carol Verheecke-Vaessen, Ranajit Bandyopadhyay, Angel Medina, Alejandro Ortega-Beltran, Naresh Magan","doi":"10.1093/lambio/ovaf072","DOIUrl":"10.1093/lambio/ovaf072","url":null,"abstract":"Maize, groundnut, and sorghum are important staple crops in several countries, but are prone to mycotoxin contamination. In the tropics and subtropics, Aspergillus flavus frequently contaminates those crops with aflatoxins and, sometimes, with cyclopiazonic acid (CPA). However, some genotypes cannot produce one or both toxins. In various countries, atoxigenic isolates of A. flavus are formulated into biocontrol products for field use to outcompete aflatoxin producers. The products effectively limit aflatoxin but their utility to reduce CPA remains unexplored. The abilities of four atoxigenic isolates (AF-) from Burkina Faso to control CPA by an isolate with high capacity to produce aflatoxins (AF+) and CPA was tested in co-inoculations at varying ratios (100+, 75+/25-, 50+/50-, 25+/75-, 100-), under simulated abiotic stress conditions. Experiments were conducted on 2% sorghum-based media at 0.95 and 0.90 water activity (aw), at 30°C and 37°C, for 12 days. CPA was quantified using liquid chromatography tandem mass spectroscopy. CPA concentrations gradually decreased as the proportion of atoxigenic isolates increased, with effectiveness varying depending on the environmental conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving maize growth through biopriming with seed endophytic Bacillus tequilensis LRB17. 龙舌兰内生芽孢杆菌LRB17生物雾化促进玉米生长

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf074

Soumya Sephalika Swain, Madhusmita Ghana, Shubhransu Nayak

{"title":"Improving maize growth through biopriming with seed endophytic Bacillus tequilensis LRB17.","authors":"Soumya Sephalika Swain, Madhusmita Ghana, Shubhransu Nayak","doi":"10.1093/lambio/ovaf074","DOIUrl":"10.1093/lambio/ovaf074","url":null,"abstract":"Maize (Zea mays L.) is the third most cultivated crop globally after wheat and rice. It is one of the important staple food crops in the tribal-inhabited forest areas in India, where reduced yield is observed due to lower soil nutrients. Biopriming with bacteria having plant growth promotion (PGP) effects could be an effective way to mitigate this problem. In this context, a potential Bacillus tequilensis LRB17 strain was bioprimed in maize cultivar for the first time for PGP. Treatment of maize with the bacterium could enhance seedling vigor by 19.56%. The root and shoot length and the root and shoot weights were enhanced by 120.21% and 121.41% and 43.03% and 40.07%, respectively. Average height of matured plants, average leaf area, and plant biomass could be enhanced by 19.30%, 30.47%, and 311.30%, respectively. Parenchyma cells in stems increased with well-developed bundle sheaths. The bacterium has been isolated from local millet crop landrace of \"Koraput\" area of the \"Eastern Ghats\" of India, which has been designated as \"Globally Important Agricultural Heritage System (GIAHS)\" by the United Nations. Hence, the native Bacillus tequilensis LRB17 posed as a potential bioagent for yield enhancement of maize for the benefit of tribal population.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila upregulates the expression of γ-aminobutyric acid in response to deoxycholate exposure. 嗜muciniphila在脱氧胆酸暴露下上调γ-氨基丁酸的表达。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf077

Tatsuro Hagi, Masaru Nomura, Clara Belzer

{"title":"Akkermansia muciniphila upregulates the expression of γ-aminobutyric acid in response to deoxycholate exposure.","authors":"Tatsuro Hagi, Masaru Nomura, Clara Belzer","doi":"10.1093/lambio/ovaf077","DOIUrl":"10.1093/lambio/ovaf077","url":null,"abstract":"Akkermansia muciniphila is a beneficial gut bacterium because of its improving metabolic effect. However, it is not fully understood how A. muciniphila interacts with host substances to inhabit the human gut. To examine the effect of deoxycholate (DCA) produced by the combination of host and gut bacteria, which enhances the growth of A. muciniphila, on the metabolic changes of A. muciniphila using transcriptome and proteome analyses. Transcriptome analysis showed that carbohydrate metabolism, including glycosyl hydrolase activity and glycosyl bond activity, was significantly upregulated. Notably, transcriptome and proteome analyses demonstrated that the γ-aminobutyric acid (GABA) production pathway, which is related to acid or osmotic stress responses, was upregulated in the presence of DCA. Our results demonstrated that carbohydrate metabolism and GABA production were altered in response to DCA. Therefore, DCA may be a key intestinal substance for the physiological regulation and persistence of A. muciniphila in the gut. This study provides valuable insights into understanding the interaction between host and gut bacterium to persist in the gut.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced recombinase polymerase amplification via UvsX engineering and reaction optimization for rapid detection of Vibrio parahaemolyticus. 通过UvsX工程增强重组酶聚合酶扩增和反应优化快速检测副溶血性弧菌。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-31 DOI: 10.1093/lambio/ovaf081

Lin Wang, Yiming Li, Pengbo Wang, Yibei Zhang, Qin Liu

{"title":"Enhanced recombinase polymerase amplification via UvsX engineering and reaction optimization for rapid detection of Vibrio parahaemolyticus.","authors":"Lin Wang, Yiming Li, Pengbo Wang, Yibei Zhang, Qin Liu","doi":"10.1093/lambio/ovaf081","DOIUrl":"https://doi.org/10.1093/lambio/ovaf081","url":null,"abstract":"Recombinase polymerase amplification (RPA) is a powerful isothermal nucleic acid amplification technique, yet its efficiency is critically dependent on the catalytic efficiency of the recombinase UvsX, a key enzyme mediating homologous DNA pairing and strand exchange. To address this limitation, in this study, we developed a specific, sensitive, and robust RPA detection method by optimizing the UvsX enzyme through protein engineering and refining the RPA reaction system. By conducting comparative structural and functional analysis of UvsX orthologs from 13 Myoviridae phages, we identified critical determinants of recombinase activity within the Loop 2 domain of T4 UvsX. Furthermore, we systematically optimized the stoichiometric ratios of core enzymes and crowding agents to establish a robust RPA system. This system was subsequently integrated with lateral flow strips (LF) for point-of-need detection of highly lethal Vibrio parahaemolyticus in shrimp. Our results demonstrated that the engineered UvsXv1 variant exhibited significantly improved strand displacement activity, leading to enhanced RPA amplification efficiency and stability.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A two-group quadruplex real-time quantitative PCR assay for molecular patho-serotyping of Yersinia enterocolitica. 结肠炎耶尔森菌分子病理分型的两组四重实时定量PCR方法。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-30 DOI: 10.1093/lambio/ovaf079

Jing Wang, Jingliang Qin, Bin Hu, Yijia Wang, Boyang Cao, Xi Guo

{"title":"A two-group quadruplex real-time quantitative PCR assay for molecular patho-serotyping of Yersinia enterocolitica.","authors":"Jing Wang, Jingliang Qin, Bin Hu, Yijia Wang, Boyang Cao, Xi Guo","doi":"10.1093/lambio/ovaf079","DOIUrl":"https://doi.org/10.1093/lambio/ovaf079","url":null,"abstract":"Yersinia enterocolitica is one of the most important foodborne pathogens with significant impact on public health. It can be divided into six biotypes and approximately 60 O-serotypes, with O:3, O:9, O:8 and O:5,27 being predominantly associated with human yersiniosis. We present a two-group quadruplex real-time quantitative PCR (RT‒qPCR) for the patho-serotyping of Y. enterocolitica. The design of primers and probes within group 1 was based on sero-specific genes within the O antigen gene cluster, and those within group 2 were selected from the virulence markers and the restriction modification system to distinguish O:5,27 from O:5. The specificity was tested using reference strains, and was confirmed by a comparison with those obtained by a previous multiplex PCR. The limit of detection is 0.1 ng, or 104 copies μl-1 of genomic DNA, and the standard curves exhibited high linearity and correlation coefficients, demonstrating our assay's robustness. Among the 81 isolates used to evaluate the reproducibility, the results for 76 were consistent between the two approaches, indicating that the sensitivity of our RT‒qPCR is 100%, and the positive predictive value is 94%. Our assay can serve as a tool for identifying sources of Y. enterocolitica contamination and for epidemiological monitoring of this bacterium.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutagenesis and transcriptomic analysis of a highly pathogenic Alternaria alternata DT-DYLC against Chenopodium album L. 高致病性交替稻瘟病菌DT-DYLC对Chenopodium album L的诱变及转录组学分析

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-30 DOI: 10.1093/lambio/ovaf080

Yuzhe Wang, Haiyang Cheng, Hongyu Cheng, Juan Li, Xin Wang, Haixia Zhu, Youhai Wei, Liang Cheng

引用次数: 0

In vitro and in-planta bioefficacy of bacteriome isolated from diseased leaf lesions of two contrasting rice genotypes against sheath blight disease of rice. 两种不同基因型水稻叶片病变菌群对水稻纹枯病的体外和体内生物药效研究

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-22 DOI: 10.1093/lambio/ovaf078

Indrajeet Usha, Devanna Pramesh, Adke Raghunandana, M K Prasannakumar, G Sunkad, Channappa Manjunatha

{"title":"In vitro and in-planta bioefficacy of bacteriome isolated from diseased leaf lesions of two contrasting rice genotypes against sheath blight disease of rice.","authors":"Indrajeet Usha, Devanna Pramesh, Adke Raghunandana, M K Prasannakumar, G Sunkad, Channappa Manjunatha","doi":"10.1093/lambio/ovaf078","DOIUrl":"https://doi.org/10.1093/lambio/ovaf078","url":null,"abstract":"Rice leaves harbor a diverse agriculturally important bacteriome. Using different dilutions and media, 20 bacterial isolates were obtained from the diseased and healthy leaves of two rice cultivars (cv. BPT-5204 and cv. Tetep). Later, the taxonomy was deduced based on the 16S rDNA sequences, which revealed 17 bacterial species belonging to 13 genera. The antifungal activity of 17 bacteria was tested against Rhizoctonia solani, which showed that Bacillus cereus, Stenotrophomonas maltophilia, and Acinetobacter lwoffii exhibited the mycelial inhibition of 65.18%, 61.85%, and 61.85%, respectively. Further, six selected bacteria (treatments: T1-T6) in three different methods of inoculation (methods: M1-M3) were tested on-field against sheath blight (ShB) for two consecutive Kharif seasons (2022 and 2023). The two-season data indicated a statistically insignificant effect of methods on the percent disease index (PDI) (40.51-42.47), whereas different bacteria treatments (T1-T6) showed a significant impact on PDI (29.15-52.08). S. maltophilia isolated from the diseased BPT-5204 was superior to all other strains in reducing the PDI (29.15). We successfully isolated and functionally characterized 17 bacteria from the artificially diseased contrasting genotypes. This study demonstrated that rice lesions naturally harbor a diverse bacterium with antifungal effects that can be used to develop non-chemical-based disease management strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual role of Bacillus thuringiensis RZ2MS9: its effectiveness as an entomopathogen and a plant growth promoter in vitro and in field conditions. 苏云金芽孢杆菌RZ2MS9在体外和田间条件下的双重作用：昆虫病原和植物生长促进剂。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-21 DOI: 10.1093/lambio/ovaf076

Daniel Prezotto Longatto, Adriana Sturion Lorenzi, Helena Gutierrez Oliveira, Mauricio Santos Rosa, Joelma Marcon, João Lucio Azevedo, Maria Carolina Quecine

{"title":"The dual role of Bacillus thuringiensis RZ2MS9: its effectiveness as an entomopathogen and a plant growth promoter in vitro and in field conditions.","authors":"Daniel Prezotto Longatto, Adriana Sturion Lorenzi, Helena Gutierrez Oliveira, Mauricio Santos Rosa, Joelma Marcon, João Lucio Azevedo, Maria Carolina Quecine","doi":"10.1093/lambio/ovaf076","DOIUrl":"https://doi.org/10.1093/lambio/ovaf076","url":null,"abstract":"Bacillus thuringiensis RZ2MS9, a plant growth-promoting rhizobacterium isolated from the rhizosphere of guarana plants, has shown significant growth enhancement in both soybean and maize crops. To explore its full potential, we investigated RZ2MS9's entomopathogenic properties against Lepidoptera and Coleoptera larvae in vitro, and against Hemiptera populations in both in vitro and field conditions. The strain was evaluated for Insecticidal Crystal Proteins, which were found in cuboid and spherical forms. Comparative genomic analysis revealed that while RZ2MS9 did not share significant pesticidal protein genes with B. thuringiensis HD1, it has unique genes related to plant growth promotion and nutrient acquisition. RZ2MS9 also exhibited chitinolytic activity, linked to the presence of the chitosanase coding gene (chP). Under laboratory conditions, the larvae mortality under RZ2MS9 treatment were 90% for sugarcane borer (Diatraea saccharalis), 92.5% for old-world cotton bollworm (Helicoverpa armigera), 30% for Agrotis ipsilon, and 87.5% for Anthonomus grandis. In addition, RZ2MS9 caused severe wing deformities in 40% of Spodoptera frugiperda moths and repelled Piezodorus guildinii from its food source. In field trials, soybean plants inoculated with RZ2MS9 exhibited significative increased length. P. guildinii was more prevalent in the inoculated plants, while the incidence of Euchistus heros remained unchanged.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-locus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme. 新兴呼吸道病原体双形支原体的多位点序列分型方案。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-20 DOI: 10.1093/lambio/ovaf073

Lily Simpson, Joshua Macleod, Richard S Rowlands, Owen B Spiller, Charlotte Dixon, Jorgen S Jensen, Baharak Afshar, Helena M B Seth-Smith, Victoria J Chalker, Michael L Beeton

{"title":"A multi-locus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme.","authors":"Lily Simpson, Joshua Macleod, Richard S Rowlands, Owen B Spiller, Charlotte Dixon, Jorgen S Jensen, Baharak Afshar, Helena M B Seth-Smith, Victoria J Chalker, Michael L Beeton","doi":"10.1093/lambio/ovaf073","DOIUrl":"https://doi.org/10.1093/lambio/ovaf073","url":null,"abstract":"Mycoplasma amphoriforme is an emerging respiratory pathogen for which little is known about the population structure or transmission dynamics. In this study, we developed the first multilocus sequence typing (MLST) scheme for M. amphoriforme and applied it to a previous genomic data set. The genomes of seven M. amphoriforme isolates from the UK and Denmark were sequenced and used to develop the MLST scheme based on loci used for previous Mycoplasma MLST schemes. The resulting MLST scheme consisted of four loci (gyrB, atpG, uvrA and rpoB) and was applied to 20 previously sequenced genomes obtained from the UK and France/Tunisia. From the 27 sequences examined, 13 sequence types were identified. A phylogenetic tree of concatenated sequences showed a comparable topology to a previously described tree based on whole genome data. Additionally, the MLST scheme corroborated the previous suggestion of possible healthcare-associated transmission of M. amphoriforme between two separate patients. The MLST scheme gave a population structure analysis comparable to previous whole-genome-based analyses.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0