Letters in Applied Microbiology最新文献

Exploring the Anti-Obesity Effects of Lactobacillus in C57BL/6 mice: Mechanisms, Interventions, and Future Directions.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-18 DOI: 10.1093/lambio/ovaf024

Sharoen Yu Ming Lim, E-Jayn Chong, Weng Yan Mah, Yan Pan, Chee Mun Fang, Chandrika Murugaiah, Mustafa Alshagga

{"title":"Exploring the Anti-Obesity Effects of Lactobacillus in C57BL/6 mice: Mechanisms, Interventions, and Future Directions.","authors":"Sharoen Yu Ming Lim, E-Jayn Chong, Weng Yan Mah, Yan Pan, Chee Mun Fang, Chandrika Murugaiah, Mustafa Alshagga","doi":"10.1093/lambio/ovaf024","DOIUrl":"https://doi.org/10.1093/lambio/ovaf024","url":null,"abstract":"Lactobacillus species show strong potential in fighting obesity-related inflammation and metabolic issues. Obesity causes inflammation in adipose tissue, which harms insulin sensitivity and leads to fat buildup. Lactobacillus strains like L. gasseri, L. reuteri, and L. plantarum help regulate lipid metabolism by boosting key genes, preventing fat cell formation, and encouraging fat breakdown. They also produce short-chain fatty acids (SCFAs) that improve gut health, activate metabolic pathways, and reduce inflammation. Studies in animals have shown that Lactobacillus can reduce body weight, fat, and inflammation, with L. plantarum being especially effective in improving gut microbiota and liver function. When combined with other probiotics or prebiotics, these strains work even better, enhancing lipid metabolism and reducing inflammation. These results suggest that Lactobacillus could be an effective way to manage obesity and related health problems by influencing metabolism, gut health, and inflammation. However, more research, particularly human clinical trials, is needed to confirm its potential as a dietary treatment for obesity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clonal expression and structural analysis of polylactic acid degrading enzyme S8SP from Bacillus safensis.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-18 DOI: 10.1093/lambio/ovaf025

Yujun Wang, Yuanyi Zhang, Chunwang Li, Siqi Meng, Dandan Wang, Lehui Zhao, Zhanyong Wang

{"title":"Clonal expression and structural analysis of polylactic acid degrading enzyme S8SP from Bacillus safensis.","authors":"Yujun Wang, Yuanyi Zhang, Chunwang Li, Siqi Meng, Dandan Wang, Lehui Zhao, Zhanyong Wang","doi":"10.1093/lambio/ovaf025","DOIUrl":"https://doi.org/10.1093/lambio/ovaf025","url":null,"abstract":"Polylactic acid (PLA) is one of the most popular biodegradable plastics favored over traditional plastics. However, it is more difficult to degrade than other biodegradable plastics probably due to the low species and number of PLA-degrading microorganisms degrading enzymes in the environment. Therefore, identifying PLA-degrading microorganisms and enzymes is of great significance for the popularization and application of PLA. This study identified a PLA-degrading enzyme, S8 serine peptidase (S8SP), from Bacillus safensis, and the heterologous expression of S8SP was conducted in Escherichia coli. PLA degradation ability of S8SP was investigated using scanning electron microscopy and water contact angle. The surface of S8SP-degraded PLA films showed obvious cracks and pits and exhibited improved hydrophilicity. The molecular weight of S8SP was about 42 kDa, and its optimum temperature and pH were 40 °C and 8.0, respectively. S8SP could maintain high stability in the temperature range of 30-40 °C and pH range of 7.0-9.0. Sodium ions (Na+), potassium ions (K+), Triton X-100, and Tween-80 promoted the enzyme activity of S8SP. S8SP had a high similarity degree to S8 serine peptidase from the genus Bacillus, and had the classical hydrolase-catalyzed triplet structure.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urease-Catalyzed Microbial and Enzymatic Carbonate Precipitation for Eco-Friendly Heavy Metal Remediation.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-12 DOI: 10.1093/lambio/ovaf022

Adharsh Rajasekar, Armstrong Ighodalo Omoregie, Kan Fock Kui

{"title":"Urease-Catalyzed Microbial and Enzymatic Carbonate Precipitation for Eco-Friendly Heavy Metal Remediation.","authors":"Adharsh Rajasekar, Armstrong Ighodalo Omoregie, Kan Fock Kui","doi":"10.1093/lambio/ovaf022","DOIUrl":"https://doi.org/10.1093/lambio/ovaf022","url":null,"abstract":"Heavy metal contamination significantly threatens environmental and public health, necessitating effective and sustainable remediation technologies. This review explores two innovative bioremediation techniques: Microbially Induced Calcium Carbonate Precipitation (MICP) and Enzyme-Induced Calcium Carbonate Precipitation (EICP). Both techniques show promise for immobilizing heavy metals in laboratory and field settings. MICP utilizes the metabolic activity of ureolytic microorganisms to precipitate calcium carbonate (CaCO3), sequestering heavy metals such as lead (Pb), cadmium (Cd), and arsenic (As) as stable metal-carbonate complexes. EICP, on the other hand, employs urease enzymes to catalyze calcium carbonate precipitation, offering greater control over reaction conditions and higher efficiency in environments unfavorable to microbial activity. This mini-review compares the mechanisms of MICP and EICP, focusing on factors influencing their performance, including enzyme or microbial activity, pH, temperature, and nutrient availability. Case studies illustrate their success in sequestering heavy metals, emphasizing their practical applications and environmental benefits. A comparative analysis highlights the strengths and limitations of MICP and EICP regarding cost, scalability, and challenges. This review synthesizes research to support the advancement of MICP and EICP as sustainable solutions for mitigating heavy metal contamination.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Learn from AI: The pursuit of objectivity.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-11 DOI: 10.1093/lambio/ovaf021

Fengyi Wang, Angeliki Marouli, Pisit Charoenwongwatthana, Chien-Yi Chang

{"title":"Learn from AI: The pursuit of objectivity.","authors":"Fengyi Wang, Angeliki Marouli, Pisit Charoenwongwatthana, Chien-Yi Chang","doi":"10.1093/lambio/ovaf021","DOIUrl":"https://doi.org/10.1093/lambio/ovaf021","url":null,"abstract":"Humans continuously face threats from emerging novel pathogens and antimicrobial resistant bacteria or fungi, which requires urgently and efficient solutions. Alternatively, microbes also produce compounds or chemicals highly valuable to humans of which require continuous refinement and improvement of yields. Artificial intelligence (AI) is a promising tool to search for solutions combatting against diseases and facilitating productivity underpinned by robust research providing accurate information. However, the extent of AI credibility is yet to be fully understood. In terms of human bias, AI could arguably act as a means of ensuring scientific objectivity to increase accuracy and precision, however, whether this is possible or not has not been fully discussed. Human bias and error can be introduced at any step of the research process including conducting experiments and data processing, through to influencing clinical applications. Despite AI's contribution to advance knowledge, the question remains, is AI able to achieve objectivity in microbiological research? Here, the benefits, drawbacks and responsibilities of AI utilization in microbiological research and clinical applications were discussed.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hospital environment as reservoir of Pseudomonas aeruginosa in human cases: a molecular epidemiology investigation in a hospital setting in central Italy.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-10 DOI: 10.1093/lambio/ovaf019

Adele Lombardi, Manuela Tamburro, Michela Lucia Sammarco, Giancarlo Ripabelli

{"title":"Hospital environment as reservoir of Pseudomonas aeruginosa in human cases: a molecular epidemiology investigation in a hospital setting in central Italy.","authors":"Adele Lombardi, Manuela Tamburro, Michela Lucia Sammarco, Giancarlo Ripabelli","doi":"10.1093/lambio/ovaf019","DOIUrl":"https://doi.org/10.1093/lambio/ovaf019","url":null,"abstract":"Pseudomonas aeruginosa is one of the main causes of hospital infections that are difficult to manage because of multidrug resistance (MDR). The aim of this study was to describe a molecular investigation on nineteen clinical and fourteen isolates from the environment in the Hub hospital of the Molise region, central Italy. Antimicrobial susceptibility was evaluated using BD Phoenix™ Automated Microbiology System. Pulsed-Field Gel Electrophoresis (PFGE) with SpeI, Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR), and Random Amplified Polymorphic DNA (RAPD-PCR) were performed for genotyping. All 33 P. aeruginosa showed MDR phenotype. PFGE had 0.99 discriminatory power, underlining high heterogeneity among the strains. The genetic relatedness between two human isolates (H12 and H15) from neonatal intensive care (NICU) and one environmental strain (E1) collected from siphon of sink in the delivery room was noticeable, as well as between one strain from faucet in NICU (E2) and siphon sink (E5) from delivery room. The link between H12, H15 and E1 strains was corroborated by ERIC-PCR showing epidemiological concordance, although with a lower discriminatory power. The study findings strengthened the critical correlation between clinical P. aeruginosa and environment, according to previous molecular surveys on outbreaks occurred in Italy.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the bacterial communities in the sediment water interface across different salinities in Pacific White shrimp, Penaeus vannamei by metabarcoding.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-10 DOI: 10.1093/lambio/ovaf020

Natarajan Lalitha, Vinaya Kumar Katneni, Ashok Kumar Jangam, Panjan Nathamuni Suganya, Suvana Sukumaran, Moturi Muralidhar

{"title":"Insight into the bacterial communities in the sediment water interface across different salinities in Pacific White shrimp, Penaeus vannamei by metabarcoding.","authors":"Natarajan Lalitha, Vinaya Kumar Katneni, Ashok Kumar Jangam, Panjan Nathamuni Suganya, Suvana Sukumaran, Moturi Muralidhar","doi":"10.1093/lambio/ovaf020","DOIUrl":"https://doi.org/10.1093/lambio/ovaf020","url":null,"abstract":"Microbes play an important role in food chain by metabolizing organic matter, cycling nutrients, and maintaining a dynamic equilibrium among organisms in water and sediment. The objective is to study fluctuating taxonomic microbial diversity profile in sediment-water interface at different days of culture (DOC) of Penaeus vannamei in varying salinities using Illumina MiSeq platform. Sediment samples were collected in Tamil Nadu State in India from low saline, brackish water, and high saline ponds on 30, 60, and 90 DOC. Bacterial richness and diversity in species was high in low-saline ponds. Beta diversity variation indicated more differences in bacterial composition in high and low saline ponds. The predominant phyla observed were Proteobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, Firmicutes and Cyanobacteria. High saline ponds accounted for more nitrification bacterial communities, sulfur reducing bacterial communities, sulfur oxidizing bacterial communities and high redox potential, whereas denitrification bacterial communities were high in brackishwater ponds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of resistant coliform bacteria in the intestine of pigs following flock versus individual treatment with neomycin against post-weaning diarrhoae or amoxicillin against umbilical infection.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf008

Egle Kudirkiene, Esben Østergaard Eriksen, Gang Liu, Anja Ejlersgård Christensen, Marianne Viuf Agerlin, Inge Larsen, Nicolai Rosager Weber, Ken Steen Pedersen, Jens Peter Nielsen, John Elmerdahl Olsen

{"title":"Selection of resistant coliform bacteria in the intestine of pigs following flock versus individual treatment with neomycin against post-weaning diarrhoae or amoxicillin against umbilical infection.","authors":"Egle Kudirkiene, Esben Østergaard Eriksen, Gang Liu, Anja Ejlersgård Christensen, Marianne Viuf Agerlin, Inge Larsen, Nicolai Rosager Weber, Ken Steen Pedersen, Jens Peter Nielsen, John Elmerdahl Olsen","doi":"10.1093/lambio/ovaf008","DOIUrl":"10.1093/lambio/ovaf008","url":null,"abstract":"The aim of this study was to investigate the effect of avoiding flock treatment on resistance levels in the intestine of pigs. To investigate this, studies, each in two pig herds, quantified resistant coliforms by culture method in pigs treated as flock or as individual animal treatments orally with neomycin against post-weaning diarrhea (PWD) or intramuscularly with amoxicillin to prevent umbilical infection. Individual oral treatment against PWD did not lead to a lower number or proportion of resistant coliforms compared to flock treated pigs in any of the two herds under study, despite reduction of treatment incidences to 32% and 35% of pigs. After intramuscular treatment against umbilical infection, fewer resistant bacteria were seen in individually treated piglets in a herd with low treatment incidence (33%), while no reduction of resistant coliform bacteria compared to flock treatment was observed in the other herd with higher treatment incidence (86%). Thus, individual animal treatment reduced the amount of antimicrobial used, but concurrent reduction in resistant coliform bacteria was not always observed.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf012

Yuchun Liu, Rui Zhu, Dongmei Liu, Yue Hu, Qing Xia, Xinwei Liu, Chunxia Wang, Yongwei Li

{"title":"The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model.","authors":"Yuchun Liu, Rui Zhu, Dongmei Liu, Yue Hu, Qing Xia, Xinwei Liu, Chunxia Wang, Yongwei Li","doi":"10.1093/lambio/ovaf012","DOIUrl":"10.1093/lambio/ovaf012","url":null,"abstract":"Herbaspirillum species are typically isolated from plants and are known for their role in crop nitrogen fixation. Recently, they have been found to colonize humans and cause infections. This study aimed to identify and characterize two Herbaspirillum huttiense strains (CLJ01 and CLJ02) isolated from the blood of a uremic patient, with a focus on evaluating their pathogenicity and antibiotic efficacy. The strains were identified using the VITEK2 system, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry , and 16S rRNA gene sequencing, confirming their identity as H. huttiense. High-throughput sequencing further revealed the presence of the resistance gene arnA, which confers resistance to polymyxin. For the first time, the pathogenicity of H. huttiense was assessed using a Galleria mellonella infection model. The results demonstrated a concentration-dependent virulence, with CLJ01 exhibiting slightly higher pathogenicity. Additionally, meropenem showed significant antimicrobial efficacy in the G. mellonella infection model, particularly under conditions of high bacterial load, indicating strong therapeutic potential. In conclusion, this study provides experimental evidence supporting the correct diagnosis and treatment of H. huttiense infections. Furthermore, the findings underscore the importance of accurately identifying rare pathogens in clinical settings for effective treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf013

Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling

{"title":"Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater.","authors":"Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling","doi":"10.1093/lambio/ovaf013","DOIUrl":"10.1093/lambio/ovaf013","url":null,"abstract":"Sumiling Dam in Sarrat, Ilocos Norte, is home to diverse phytoplankton species. This study aims to isolate and identify phytoplankton species, optimize the cultivation of one phytoplankton under varying light, pH, and temperature conditions, and assess its bioremediation capacity in aquaculture wastewater. Three stations were set up at the site, each with three sampling points spaced 3-5 m apart. From each point, 300 ml of water was collected using standard procedures and transported to the lab for phytoplankton identification. Samples were incubated in algae culture broth for 14 days, streak-plated for isolation, and cultured under light. Microscope examination was used to characterize phytoplankton species, and the identification was verified by a professional. Ten phytoplankton species were identified, with Haematococcus sp. selected for wastewater bioremediation due to its optimal growth under specific conditions (81 µmol m-2 s-1, pH 9, 25°C ± 2°C). Experiments showed that Haematococcus sp. significantly reduced nitrogen and phosphorus in wastewater over 7-14 days, with Treatment 3 (1000 mg/l) achieving the highest growth, chlorophyll, and nitrogen reduction, while Treatment 2 (750 mg/l) excelled in phosphorus removal. These results underscore phytoplankton's potential for sustainable, large-scale wastewater bioremediation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between pathogenic bacteria and different stages of colorectal cancer.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf017

Javad Nezhadi, Hossein Samadi Kafil, Mohammad Sadrkabir, Farshad Mahdavi, Seyed Yaghoub Moaddab, Roghayeh Nouri, Yalda Mohammadzadeh-Asl, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee

{"title":"The relationship between pathogenic bacteria and different stages of colorectal cancer.","authors":"Javad Nezhadi, Hossein Samadi Kafil, Mohammad Sadrkabir, Farshad Mahdavi, Seyed Yaghoub Moaddab, Roghayeh Nouri, Yalda Mohammadzadeh-Asl, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee","doi":"10.1093/lambio/ovaf017","DOIUrl":"10.1093/lambio/ovaf017","url":null,"abstract":"Colorectal cancer (CRC) involves uncontrolled cell growth in the colon and rectum. This study aims to explore the prevalence of key pathogenic bacteria and their role in the progression of CRC, focusing on microbial dysbiosis. This study analyzed 52 stool and tissue samples through polymerase chain reaction (PCR), real-time PCR, and bioinformatics to identify associations between pathogenic bacteria and CRC progression. PCR results revealed a significant association between the Bacteroides fragilis toxin (bft) gene and CRC progression (P = 0.001, r = 0.570). Furthermore, Real-time PCR showed significant differences in the frequency of pks+Escherichia coli in CRC stages 1 (P = 0.03), 2 (P = 0.004), and 3 (P = 0.0002) compared to the control group. Additionally, the frequency of Fusobacterium nucleatum in stage 3 CRC patients was significantly higher than in the control group (P = 0.004) and stage 1 patients (P = 0.01). Furthermore, Streptococcus gallolyticus showed similar significant differences in stage 3 patients (P = 0.004). Bioinformatics analyses using KEGG, Reactome, STRING, and dbSNP highlighted bacteria's roles in colorectal carcinogenesis, emphasizing the need for early identification and management in CRC treatment and prevention strategies. Finally, due to the limitations of the study, the use of more advanced methods and the validation of results through more reliable techniques are essential for future research.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0