Letters in Applied Microbiology最新文献

Enhancing blueberries' safety: antimicrobial effects of alkyltrimethylammonium bromide against foodborne pathogens. 提高蓝莓的安全性：烷基三甲溴化铵对食源性病原体的抗菌作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf042

Syed Ammar Hussain, Majher I Sarker, Tony Z Jin

引用次数: 0

Bioprospecting for probiotics and other technological aspects of the bacteria isolated from goat milk. 羊奶中益生菌及其分离技术的生物勘探。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf050

Namita Ashish Singh, Jyoti, Shakshi, Vidhi Jain, Jaya, Nitish Rai, Rahul Jain

{"title":"Bioprospecting for probiotics and other technological aspects of the bacteria isolated from goat milk.","authors":"Namita Ashish Singh, Jyoti, Shakshi, Vidhi Jain, Jaya, Nitish Rai, Rahul Jain","doi":"10.1093/lambio/ovaf050","DOIUrl":"10.1093/lambio/ovaf050","url":null,"abstract":"Goat milk is a superior nutritional source for human beings. It possesses probiotic, prebiotic, immunomodulatory actions and inhibits the adherence of pathogenic bacteria. The microbiota of raw goat milk is a rich source of novel bacteriocin-producing lactic acid bacteria (LAB). LAB were evaluated for their probiotic attributes, i.e. acid, bile tolerance, and safety assessment. The antimicrobial activity of LAB isolated from goat milk was assessed against the 10 most common indigenous bacterial pathogens confirmed through 16S rRNA sequencing. The strains LAB GM8 and GM121 showed high tolerance to acidic pH while GM121 showed tolerance to high bile salt concentrations. LAB GM121 showed susceptibility to eight antibiotics which meets the safety requirements regarding phenotypic resistance evaluation. Out of 10, 4 LAB GM8, GM26, GM121, GM122 displayed antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus, demonstrating their ability to produce bioactive compounds. So, based on acid, bile tolerance, and safety requirements, it can be concluded that the GM121 has the potential to be explored as probiotic strains in the dairy industry. Bacterial isolates (n-24) were screened for exopolysaccharide production and amylase activity; Bacillus paralicheniformis GM75 showed good exopolysaccharide production and antimicrobial activity while Bacillus cereus GM56 showed maximum amylase activity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of drugs with potential antifungal activity for repurposing in the treatment of cryptococcosis. 隐球菌病治疗中潜在抗真菌活性药物的筛选。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf045

Emanuel Almeida Moreira de Oliveira, Gabriella Freitas Ferreira, Karen Luise Lang

{"title":"Screening of drugs with potential antifungal activity for repurposing in the treatment of cryptococcosis.","authors":"Emanuel Almeida Moreira de Oliveira, Gabriella Freitas Ferreira, Karen Luise Lang","doi":"10.1093/lambio/ovaf045","DOIUrl":"10.1093/lambio/ovaf045","url":null,"abstract":"Current therapeutic alternatives for the treatment of cryptococcosis are scarce, highly toxic, expensive, and difficult to access. Therefore, the aim of this study was to evaluate the anticryptococcal potential of a collection of 27 drugs in vitro against several strains of Cryptococcus neoformans and Cryptococcus gattii. We investigated several parameters to evaluate the antifungal activity of the drugs: determination of minimum inhibitory concentration (MIC), combinatorial effects with fluconazole, kinetics of growth inhibition, post-antifungal effect (PAFE), and morphometric analyses at subinhibitory concentrations. Antiparasitics albendazole, fenbendazole, flubendazole, mebendazole, and antidepressants duloxetine and paroxetine showed antifungal activity with an MIC of 100 µmol l-1 or less for most strains tested. The results of the zero-interaction power model indicated additive effects for combination of fluconazole with drugs finasteride, hydroxyzine, and paroxetine. The combined treatments significantly improved the ability of fluconazole to kill C. neoformans ATCC H99. Same phenomenon occurred in the in vitro PAFE, as the combinations suppressed fungal growth more effectively than fluconazole alone. A significant reduction in capsule size was observed. Screening of the drug collection showed interesting results, with benzimidazoles antiparasitics and serotonin and norepinephrine reuptake inhibitors in foreground. Finasteride, hydroxyzine, and paroxetine significantly improved activity of fluconazole in vitro.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial consortia of ewes' whey in the production of bryndza cheese in Slovakia. 斯洛伐克bryndza奶酪生产中母羊乳清的细菌联合体。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf047

Nikola Klištincová, Janka Koreňová, Zuzana Rešková, Zuzana Čaplová, Alexandra Burdová, Zuzana Farkas, Martin Polovka, Hana Drahovská, Domenico Pangallo, Tomáš Kuchta

{"title":"Bacterial consortia of ewes' whey in the production of bryndza cheese in Slovakia.","authors":"Nikola Klištincová, Janka Koreňová, Zuzana Rešková, Zuzana Čaplová, Alexandra Burdová, Zuzana Farkas, Martin Polovka, Hana Drahovská, Domenico Pangallo, Tomáš Kuchta","doi":"10.1093/lambio/ovaf047","DOIUrl":"10.1093/lambio/ovaf047","url":null,"abstract":"Whey from previous production is often used as a natural starter in the technology of traditional cheeses, including bryndza cheese in Slovakia. Therefore, studying its bacterial community and isolating new potential natural starters is important for improving the characteristics of the final product. Composition of bacterial consortia of fresh and fermented whey in the production of raw ewes' milk-based bryndza cheese from 8 small or medium-sized producers was analysed. Culture-based microbiological analysis and culture-independent analysis based on 16S rRNA gene sequencing by MiSeq and MinION were used. Results showed the dominance of lactococci or streptococci, with 3-8 log CFU ml-¹ of Lactobacillus sensu lato in all whey samples. Potential natural starters comprising Lacticaseibacillus paracasei/casei, Lactiplantibacillus plantarum, Lentilactobacillus parabuchneri, Lactobacillus helveticus, L. diolivorans, Levilactobacillus brevis, Limosilactobacillus fermentum, L. delbrueckii, L. gasseri and L. otakiensis were isolated. Coliforms were also present in all samples, with no consistently lower values in fermented whey samples. Some samples contained pseudomonads and/or acinetobacters. Coagulase-positive staphylococci were present at relevant levels in samples from 4 producers. The results revealed that whey is a source of natural starters due to the presence of lactobacilli.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of multiplex PCR for detection of foodborne pathogens in fresh produce. 多重PCR检测新鲜农产品食源性致病菌的研究进展。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf038

Ian Moppert, Eiseul Kim, Seung-Min Yang, Si Hong Park

{"title":"Development of multiplex PCR for detection of foodborne pathogens in fresh produce.","authors":"Ian Moppert, Eiseul Kim, Seung-Min Yang, Si Hong Park","doi":"10.1093/lambio/ovaf038","DOIUrl":"10.1093/lambio/ovaf038","url":null,"abstract":"Foodborne pathogens present a significant public health concern where fresh produce is a key agricultural product. Rapid and sensitive detection methods are essential to ensure the safety of such produce. This study aimed to develop and optimize a modified multiplex polymerase chain reaction (mPCR) assay, which incorporates enhancements to conventional PCR, for the simultaneous detection of Escherichia coli, Salmonella, and Listeria monocytogenes in fresh produce. The specificity of each primer pair was validated using 15 strains, confirming 100% accurate detection of pathogenic strains without cross-reactivity. Since no false positives were observed, the assay demonstrated 100% precision, highlighting its reliability in distinguishing target pathogens. The sensitivity of the mPCR assay was demonstrated through serial dilutions, detecting Salmonella down to 10 fg µl-1, L. monocytogenes to 100 fg µl-1, and E. coli to 1 pg µl-1. The mPCR assay was then successfully applied to romaine lettuce and kale, demonstrating its effectiveness in detecting pathogens in mixed samples inoculated at varying concentrations (109-101 CFU ml-1). Kale exhibited greater sensitivity, detecting pathogens at lower levels, while romaine lettuce also provided consistent detection. This study highlights the potential of mPCR for enhancing food safety by providing rapid and sensitive pathogen detection in fresh produce.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free supernatant of Clostridium leptum inhibits breast cancer cell proliferation. 瘦梭菌无细胞上清液抑制乳腺癌细胞增殖。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf037

Qingling Wang, Huan Zhao, Huizhe Ding, Hao Zhang, Jizhou Zhang, Liqin Li, Bing Han, Guoyin Kai

{"title":"Cell-free supernatant of Clostridium leptum inhibits breast cancer cell proliferation.","authors":"Qingling Wang, Huan Zhao, Huizhe Ding, Hao Zhang, Jizhou Zhang, Liqin Li, Bing Han, Guoyin Kai","doi":"10.1093/lambio/ovaf037","DOIUrl":"10.1093/lambio/ovaf037","url":null,"abstract":"Breast cancer has emerged as the leading cause of global cancer incidence, surpassing lung cancer. Accumulating evidence suggests that probiotics exhibit inhibitory effect on breast cancer progression, highlighting the need to identify gut flora-derived probiotics with potential anti-breast cancer properties. Here, we investigated the effect of the cell-free supernatant of Clostridium leptum (ClCFS) on breast cancer cells by methyl thiazolyl tetrazolium (MTT) assay. Untargeted metabolomics analysis was employed to characterize metabolite alterations in ClCFS. Furthermore, the core targets were predicted by the protein-protein interaction network and the signaling pathways were enriched by the Kyoto Encyclopedia of Genes and Genomes analysis. Our findings demonstrated that ClCFS inhibited the proliferation of breast cancer cells and that the main metabolite of ClCFS might be acetylcarnitine. Utilizing network pharmacological analysis, we identified apoptosis-related signaling pathways as the principal mechanisms underlying ClCFS activity. Furthermore, five core targets of STAT3, IL-1β, BCL2, CASP3, and ESR1 were identified. This study elucidates the main bioactive constituent and the potential targets of ClCFS against breast cancer. It provides a new understanding of the pharmacological activity of ClCFS in breast cancer treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome sequencing and phylogenetic relative of a pure virulent Newcastle disease virus isolated from an outbreak in northeast Iran. 从伊朗东北部一次疫情中分离出的一种纯新城疫病毒的全基因组测序和系统发育亲缘关系。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf049

Aliakbar Khabiri, Reza Toroghi, Mohammadreza Mohammadabadi, Seyed-Elias Tabatabaeizadeh

{"title":"Whole genome sequencing and phylogenetic relative of a pure virulent Newcastle disease virus isolated from an outbreak in northeast Iran.","authors":"Aliakbar Khabiri, Reza Toroghi, Mohammadreza Mohammadabadi, Seyed-Elias Tabatabaeizadeh","doi":"10.1093/lambio/ovaf049","DOIUrl":"https://doi.org/10.1093/lambio/ovaf049","url":null,"abstract":"Newcastle Disease (ND) is a significant viral disease affecting poultry worldwide, with outbreaks persisting despite extensive vaccination efforts. This study characterizes a novel NDV strain, RT40, isolated from poultry farms in northeast Iran for the first time. RT40, classified as genotype VII, subgenotype VII.1.1, has a genome length of 15 192 nucleotides, with six genes, 12 UTRs, and five IGS, consistent with class II NDVs. Pathogenicity tests confirm its virulent velogenic nature, differentiating it from other known strains. Phylogenetic analysis points to a Western origin, suggesting possible cross-border transmission with Iraqi strains, highlighting the strain's relevance for NDV surveillance. Virus isolation, RNA sequencing, and cross-border transmission analysis were performed, revealing that RT40 shares a high nucleotide sequence identity with Iraqi strains, further supporting cross-border transmission concerns. The findings underscore the need for genotype-specific vaccines tailored to circulating strains. The genome, deposited in GenBank (accession ON184061), serves as an essential resource for future research and vaccine development. By emphasizing whole-genome sequencing, this study aims to improve NDV evolution tracking and enhance understanding of NDV diversity. This work calls for targeted vaccine strategies to effectively manage NDV spread, ensuring the sustainability of the poultry industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human gut microbiome and sleep across adulthood: associations and therapeutic potential. 人类肠道微生物群和成年期睡眠：关联和治疗潜力。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf043

Jacob Cavon, Melissa Basso, Kathrin Cohen Kadosh, Sean M Gibbons

引用次数: 0

Characterization, antifungal activity, and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains. 菝葜精油对假丝酵母菌和新型隐球菌的抑菌活性及其可能的作用机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf040

Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior

{"title":"Characterization, antifungal activity, and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains.","authors":"Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior","doi":"10.1093/lambio/ovaf040","DOIUrl":"10.1093/lambio/ovaf040","url":null,"abstract":"This study aimed to characterize Melissa officinalis essential oil (EOMO) from a region in Northeast Brazil and evaluate its antifungal activity against Candida and Cryptococcus neoformans, by analyzing its action mechanism. EOMO was characterized using gas chromatography-mass spectrometry and the main chemical compounds were Geranial (14.10%) and (Z)-Nerolidol (17.75%). The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) against strains of Candida albicans, Candida parapsilosis, Candida krusei, Candida auris (MIC raging from 256 to 26.7 µg ml-1) and C. neoformans (MIC ranging from 64 to 32 µg ml-1). Flow cytometry and comet assays were employed to investigate EOMO's mechanism of action, which might be related to an increase in the production of reactive oxygen species and damage to fungal DNA. The chemical composition of EOMO from Northeast Brazil showed a higher content of (Z)-Nerolidol and has significant antifungal potential.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caffeine as a Natural Inhibitor of Salmonella Biofilms in Fruit Juices. 咖啡因作为果汁中沙门氏菌生物膜的天然抑制剂。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf053

André Ricardo Peron Dos Santos, Bruna Camila Souza Lima, Gabriel José Couto, Luana de Carvalho, Lucília Rocha Magna, Matheus Henrique Nogueira, Mariana Lima Braga, Milena Matesco Carreteiro, Márcia Cristina Furlaneto, Luciana Furlaneto Maia

{"title":"Caffeine as a Natural Inhibitor of Salmonella Biofilms in Fruit Juices.","authors":"André Ricardo Peron Dos Santos, Bruna Camila Souza Lima, Gabriel José Couto, Luana de Carvalho, Lucília Rocha Magna, Matheus Henrique Nogueira, Mariana Lima Braga, Milena Matesco Carreteiro, Márcia Cristina Furlaneto, Luciana Furlaneto Maia","doi":"10.1093/lambio/ovaf053","DOIUrl":"https://doi.org/10.1093/lambio/ovaf053","url":null,"abstract":"Caffeine holds promise for applications in food safety due to its antioxidant and antibacterial properties. Given rising antimicrobial resistance, its natural antimicrobial potential is valuable for controlling foodborne pathogens and reducing reliance on synthetic preservatives. This study aimed to explore caffeine as an alternative to control Salmonella biofilms in fruit juice substrates. S. Enteritidis and S. Typhimurium biofilm were developed in Brain Heart Infusion (BHI) broth (control), grape and apple juice, before and after caffeine application. Biofilm inhibition was quantified by crystal violet staining, exopolysaccharide (EPS) production, and visualization through Confocal and Scanning Electron Microscopy. Swimming motility assays assessed caffeine's impact on bacterial motility. Both strains formed biofilms in the tested juices. The Minimum Inhibitory Concentration (MIC) of caffeine was 9.37 mM ml-1 for S. Typhimurium and 18.75 mM ml-1 for S. Enteritidis. Biofilm inhibition was observed for treatments before and after caffeine application, with varying levels depending on the matrix. EPS production and inhibition were higher in biofilms formed in grape and apple juices compared to the control (BHI). Sub-inhibitory concentrations of caffeine reduced motility in both strains. These findings suggest that caffeine may be a promising approach to control Salmonella biofilms in the food industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0