Letters in Applied Microbiology最新文献

Protective effects of Rasa Manikya and Rasa Sindura against drug-resistant Salmonella Typhimurium sepsis in mice. Rasa Manikya和Rasa Sindura对小鼠耐药鼠伤寒沙门氏菌败血症的保护作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-19 DOI: 10.1093/lambio/ovag089

Manas Das, Jaydeep Banerjee, Prasanta Kumar Sarkar, Samiran Mondal, Suman Biswas, Pramod Kumar Nanda, Arun Kumar Das, Ujjwal Kumar De, Arnab Sen, Sameer Shrivastava, Sanjay Kumar Singh, Dhruba Jyoti Sarkar, Asit K Bera, Samiran Bandyopadhyay

{"title":"Protective effects of Rasa Manikya and Rasa Sindura against drug-resistant Salmonella Typhimurium sepsis in mice.","authors":"Manas Das, Jaydeep Banerjee, Prasanta Kumar Sarkar, Samiran Mondal, Suman Biswas, Pramod Kumar Nanda, Arun Kumar Das, Ujjwal Kumar De, Arnab Sen, Sameer Shrivastava, Sanjay Kumar Singh, Dhruba Jyoti Sarkar, Asit K Bera, Samiran Bandyopadhyay","doi":"10.1093/lambio/ovag089","DOIUrl":"https://doi.org/10.1093/lambio/ovag089","url":null,"abstract":"This study investigated the in vitro antibacterial efficacy of classical herbo‑mineral formulations, Rasa Manikya (RM) and Rasa Sindura (RS), and also evaluated their ameliorative potential in combating multidrug‑resistant (MDR) Salmonella Typhimurium -induced sepsis in a murine model. Both RM and RS exhibited dose‑dependent antibacterial activity in agar well diffusion and broth microdilution assays. Oral administration of RM and RS to mice at a dose of 25 mg/kg body weight significantly (p < 0.05) improved clinical scores, restored haematological and biochemical parameters, and moderately reduced bacterial burden in liver, spleen, and kidney, compared to the healthy group. RM and RS treatment also rebalanced hepatic oxidant/antioxidant status and ameliorated histopathological damage in vital organs. Principal Component Analysis revealed a clear shift in treated groups toward normal physiological profiles. Analysis of survival trials using the Log-Rank (Mantel-Cox) test identified significant differences, with treated mice groups- RS (87.25%) and RM (81.25%) showing markedly enhanced survival, compared to untreated controls (50%). The ameliorative effects of RM and RS in a murine sepsis model underscore their potential as alternative therapeutics. However, further studies-particularly those addressing potential toxicity, and pharmacokinetics,-are warranted before any consideration of clinical application.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sophorose biosynthesis with heterologous beta-glucosidases for cellulase production in Trichoderma reesei. 异源β -葡萄糖苷酶在里氏木霉纤维素酶生产中的生物合成。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-14 DOI: 10.1093/lambio/ovag088

Jiaming Liu, Chunyang Zhou, Xiaolong Gao, Ke Yu, Yinhang Cai, Xuetong Cui, Peng Xiong, Xiutao Liu

{"title":"Sophorose biosynthesis with heterologous beta-glucosidases for cellulase production in Trichoderma reesei.","authors":"Jiaming Liu, Chunyang Zhou, Xiaolong Gao, Ke Yu, Yinhang Cai, Xuetong Cui, Peng Xiong, Xiutao Liu","doi":"10.1093/lambio/ovag088","DOIUrl":"https://doi.org/10.1093/lambio/ovag088","url":null,"abstract":"Sophorose is a strong soluble inducer of cellulase biosynthesis in Trichoderma reesei, but its practical use remains limited by availability and cost. In this study, an integrated strategy was developed to improve cellulase production by combining sophorose biosynthesis, sophorose-based induction, and heterologous β-glucosidase expression. β-Glucosidases from T. reesei QM6a, Penicillium digitatum Pdw03, and Aspergillus niger CBS 513.88 were expressed in Escherichia coli BL21(DE3) and compared for sophorose formation. The A. niger-derived enzyme rAnCel1A showed the highest transglycosylation activity, producing 8.42 ± 0.21 g L-1 sophorose at 47°C and showing optimal performance at pH 3.2. In a 20-L bioreactor, an enzymatically synthesized sophorose-glucose mixture containing 12.7 ± 0.8 g L-1 sophorose triggered detectable cellulase production within 24 h after induction, whereas glucose-only feeding failed to initiate cellulase production despite comparable biomass formation. Heterologous expression of fungal cel1A genes in T. reesei Rut-C30 further increased β-glucosidase and filter paper activities. The An-cel1A advantage disappeared after gene-dosage normalization, suggesting higher expression. These results provide a feasible framework for coupling inducer biosynthesis with cellulase production in T. reesei.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Last-line antimicrobial resistance genes in food-chain Enterococcus faecium sensu stricto: a 2,090-genome public-archive audit with whole-genome species validation. 食物链中的最后一种抗菌素耐药基因严格感粪肠球菌：2,090个基因组公共档案审计与全基因组物种验证。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-13 DOI: 10.1093/lambio/ovag087

Sheng Song, Yu Liu, Li Yan, BaiYu Li, LanXin Xu

{"title":"Last-line antimicrobial resistance genes in food-chain Enterococcus faecium sensu stricto: a 2,090-genome public-archive audit with whole-genome species validation.","authors":"Sheng Song, Yu Liu, Li Yan, BaiYu Li, LanXin Xu","doi":"10.1093/lambio/ovag087","DOIUrl":"https://doi.org/10.1093/lambio/ovag087","url":null,"abstract":"The extent to which food-chain bacteria carry last-line antimicrobial resistance genes (ARGs) is poorly quantified. We screened 2,090 NCBI genomes whose BioSample metadata indicated food-chain association. Mash-based validation against type strains reassigned 663 genomes (31.7%) to Enterococcus lactis (former clade B) and exposed one misidentified record; a standardized minimap2/ResFinder re-screen removed three annotation-based false-positive calls. The primary estimand was the proportion of last-line ARG (vanA, optrA, poxtA, cfr)-positive genomes within this predefined sampling frame, not population prevalence. After excluding apparent clinical admixture, 140/1,283 E. faecium sensu stricto genomes (10.91%; 95% CI 9.32-12.74) carried a last-line ARG, versus 4/646 E. lactis (0.62%; 0.24-1.58). vanA carriage concentrated in clonal complex CC17 (59/83 observed carriers), whereas optrA/poxtA carriage did not (20-28% CC17). Carriage rose from 4.8% (2013-16) to 13.1% (2021-26) among dated genomes while multi-ARG carriage declined. No carrier had measured susceptibility data; findings are genomic and archive-bound.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing microbial resistance to Artocarpus heterophyllus leaf-mediated AgNO3 colloidal aggregates using adaptive laboratory evolution. 利用适应性实验室进化评估微生物对鹿角树叶片介导的AgNO3胶体聚集体的抗性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-08 DOI: 10.1093/lambio/ovag086

Maya Fitriyanti, Almando Geraldi, Anna Rakhmawati, Karin Rosalinda, Najwa Fadhilatunnisa, Nurhamidah Almatin Hidayat, Arienda Elita Amadea

{"title":"Assessing microbial resistance to Artocarpus heterophyllus leaf-mediated AgNO3 colloidal aggregates using adaptive laboratory evolution.","authors":"Maya Fitriyanti, Almando Geraldi, Anna Rakhmawati, Karin Rosalinda, Najwa Fadhilatunnisa, Nurhamidah Almatin Hidayat, Arienda Elita Amadea","doi":"10.1093/lambio/ovag086","DOIUrl":"https://doi.org/10.1093/lambio/ovag086","url":null,"abstract":"Antimicrobial resistance poses a major global health challenge, highlighting the need for alternative antimicrobial strategies. In this study, silver-containing colloidal aggregates were synthesized using an extract from Artocarpus heterophyllus leaves and evaluated for their antimicrobial activity and effects on microbial survival under adaptive conditions. Silver-containing colloidal aggregates were characterized for size, structure, and stability, followed by antimicrobial assessment using Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) assays. The synthesized silver-containing colloidal aggregates exhibited an average size of 444.00 ± 32.84 nm, a polydispersity index of 0.81 ± 0.16, and a zeta potential of -24.28 ± 10.66 mV. Functional group analysis indicated that phytochemicals mediated the reduction of Ag⁺ ions. Antimicrobial activity was observed against Escherichia coli, Staphylococcus aureus, and Candida albicans, with MIC values of 80, 640, and 320 mg l-1, respectively, and MBC/MFC values of 1280 mg l-1 (E. coli and C. albicans) and 5120 mg l-1 (S. aureus). Adaptive laboratory evolution (ALE) experiments under gradually increasing silver-containing colloidal particle concentrations showed a progressive decline in microbial growth, with no detectable survival at higher exposure levels. These findings suggest that silver-containing colloidal aggregates exhibit antimicrobial activity and may limit short-term microbial adaptation under the tested conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impaired adaptation to smoke-derived phenolic compounds in Listeria monocytogenes CC204 from smoked salmon and trout. 来自熏鲑鱼和鳟鱼的单核增生李斯特菌CC204对烟雾衍生酚类化合物的适应性受损。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-06 DOI: 10.1093/lambio/ovag074

Marion Gouyette, Guillaume Gillot, Virginie Chesnais, Antoine Riou, Nicolas Nguyen Van Long, Thomas Brauge

{"title":"Impaired adaptation to smoke-derived phenolic compounds in Listeria monocytogenes CC204 from smoked salmon and trout.","authors":"Marion Gouyette, Guillaume Gillot, Virginie Chesnais, Antoine Riou, Nicolas Nguyen Van Long, Thomas Brauge","doi":"10.1093/lambio/ovag074","DOIUrl":"10.1093/lambio/ovag074","url":null,"abstract":"Listeria monocytogenes is a major foodborne pathogen in ready-to-eat smoked fish products. This study evaluated whether clonal complex affiliation contributes to variability in growth responses to stresses representative of smoked salmon and trout processing. Ten strains were studied, including strains from CC121, CC26, and CC204, the three major clonal complexes reported in the French smoked salmon and trout sectors, together with the EGDe reference strain. Strains were exposed to salt, cold, smoke-derived phenolic compounds, and combined stress conditions. Growth responses were compared with whole-genome-based phylogeny, and the impaired phenotype observed under phenolic exposure was further investigated using viable counts, live/dead microscopy and comparative genomics. Growth profiles were partly structured by clonal complex, with strains from the same clonal complex showing similar behaviour across stress conditions. Salt and cold reduced growth globally, while smoke-derived phenolic compounds were the most discriminating conditions. CC204 strains showed markedly lower growth rates under phenolic exposure than CC121, CC26, and EGDe. This phenotype was not associated with loss of cultivability or significant loss of membrane integrity. Comparative genomics did not identify a clear gene-content determinant explaining the CC204 phenotype. These results suggest that CC204 has an impaired adaptive response to smoke-derived compounds, likely involving regulatory or physiological mechanisms.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of lytic bacteriophages targeting clinical Staphylococcus other than S. aureus. 非金黄色葡萄球菌裂解噬菌体的分离与鉴定。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-06 DOI: 10.1093/lambio/ovag082

Gerardo Antonio Romero-Borja, Luis Enrique Jiménez-Camacho, Victoria Abigail Sánchez-Herrera, Daniel Huelgas-Méndez, Jesús Guillermo Jiménez-Cortés, Luis Esaú López-Jácome, Jossue Ortiz-Álvarez, Rafael Franco-Cendejas, Clara Estela Díaz-Velásquez, Felipe Vaca-Paniagua, Reyna Lara-Martínez, Luis Felipe Jiménez-García, Fohad Mabood Husain, Altaf Khan, Frederic Cadet, Thomas K Wood, Ana María Fernández-Presas, Rodolfo García-Contreras

{"title":"Isolation and characterization of lytic bacteriophages targeting clinical Staphylococcus other than S. aureus.","authors":"Gerardo Antonio Romero-Borja, Luis Enrique Jiménez-Camacho, Victoria Abigail Sánchez-Herrera, Daniel Huelgas-Méndez, Jesús Guillermo Jiménez-Cortés, Luis Esaú López-Jácome, Jossue Ortiz-Álvarez, Rafael Franco-Cendejas, Clara Estela Díaz-Velásquez, Felipe Vaca-Paniagua, Reyna Lara-Martínez, Luis Felipe Jiménez-García, Fohad Mabood Husain, Altaf Khan, Frederic Cadet, Thomas K Wood, Ana María Fernández-Presas, Rodolfo García-Contreras","doi":"10.1093/lambio/ovag082","DOIUrl":"10.1093/lambio/ovag082","url":null,"abstract":"Staphylococcus spp., other than S. aureus (SOSA, formerly CoNS), are opportunistic pathogens often linked to biofilm infections on indwelling devices. Their rising antibiotic resistance highlights the need for novel therapies. Bacteriophages are promising due to their specificity and ability to inhibit cell growth and biofilm formation. In this study, lytic phages isolated from swab pools were characterized against six clinical S. epidermidis and S. warneri isolates. Two distinct groups were identified via their host range, transmission electron microscopy, and genome sequencing. Group 1 phage ΘBRJ9 belongs to genus Sepunavirus (Myoviridae), while Group 2 phage ΘBRJ18 belongs to genus Andhravirus (Podoviridae). Both showed rapid adsorption, short latent periods (20 min), and high burst sizes (79 and 60 PFU/cell). They strongly inhibited planktonic SOSA growth at MOI 10, with efficacy comparable to or better than vancomycin, and their genomes lacked lysogeny, virulence, or resistance genes. These phages display a lytic lifestyle and are candidates for targeted SOSA therapies. Future work will assess biofilm efficacy, antibiotic synergies, and in vivo performance.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intensity of free radicals - dependent induction of VBNC state in Salmonella typhimurium within carrot juice. 胡萝卜汁中鼠伤寒沙门菌诱导VBNC状态的自由基强度依赖性研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-06 DOI: 10.1093/lambio/ovag081

Yilin Sun, Jingjing Yang, Ruirui Zhang, Liao Hongmei

{"title":"Intensity of free radicals - dependent induction of VBNC state in Salmonella typhimurium within carrot juice.","authors":"Yilin Sun, Jingjing Yang, Ruirui Zhang, Liao Hongmei","doi":"10.1093/lambio/ovag081","DOIUrl":"10.1093/lambio/ovag081","url":null,"abstract":"Hydrogen peroxide is widely used for microbial disinfection, but may induce bacteria into the viable but non-culturable (VBNC) state. However, it may induce bacteria into the VBNC state, and the underlying mechanisms remain unclear. This study employed hydrogen peroxide as a model oxidant to investigate VBNC induction in Salmonella typhimurium. VBNC formation showed a clear concentration-dependent relationship with hydrogen peroxide. Free radicals generated during treatment, including electron spin resonance (ESR) signals consistent with hydroxyl-, hydrogen-, and carbon-centered species, were associated with this transition. Among the observed signals, the hydroxyl-radical-related signal showed the highest apparent intensity under the tested conditions. A strong nonlinear association was observed between radical-related ESR signal intensity and VBNC incidence, and a constrained logistic model was used to quantitatively describe this relationship. Sodium pyruvate reduced the ESR signal and was associated with increased viable and culturable cell counts and decreased VBNC incidence at low to moderate H2O2 concentrations. Overall, the findings indicate that radical-mediated oxidative stress may be an important contributing factor to VBNC induction under the tested conditions, while the specific contributions of H2O2 and individual radical species require further investigation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and functional characterization of Limosilactobacillus fermentum DX2034 identifies a probiotic candidate with TLR2-associated anti-adipogenic activity. 发酵Limosilactobacillus fermentum DX2034的基因组和功能鉴定鉴定了一种具有tlr2相关抗脂肪活性的候选益生菌。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-06 DOI: 10.1093/lambio/ovag080

Do-Won Park, Ji Hyung Kim, Do-Young Park

{"title":"Genomic and functional characterization of Limosilactobacillus fermentum DX2034 identifies a probiotic candidate with TLR2-associated anti-adipogenic activity.","authors":"Do-Won Park, Ji Hyung Kim, Do-Young Park","doi":"10.1093/lambio/ovag080","DOIUrl":"10.1093/lambio/ovag080","url":null,"abstract":"Limosilactobacillus fermentum is a promising probiotic species with reported benefits for gastrointestinal health and host metabolism. In this study, a novel L. fermentum strain, DX2034, isolated from the feces of a healthy Korean infant, was characterized through phenotypic and genomic analyses, and its anti-adipogenic activity was evaluated in differentiated 3T3-L1 adipocytes. DX2034 exhibited high tolerance to simulated gastric and bile conditions and showed stronger adhesion to Caco-2 cells than the reference strain L. fermentum CECT5716. The strain showed no hemolytic activity and was susceptible to most tested antibiotics. Whole-genome sequencing revealed a single circular chromosome of 2 160 171 bp with no acquired antibiotic resistance genes, virulence-associated factors, or concerning secondary metabolite biosynthetic gene clusters. Comparative genomic analysis identified genomic regions showing low similarity to reference strains, including a putative glycosyltransferase-associated locus, and genes associated with stress adaptation, gastrointestinal persistence, surface modification, and quorum sensing. Heat-killed DX2034 significantly reduced lipid accumulation, intracellular triglyceride content, and expression of adipogenesis-related genes, including C/EBPα, PPARγ, FABP4, LPL, and FASN. These effects were attenuated by the TLR2 inhibitor C29, suggesting involvement of TLR2-associated signaling. Collectively, DX2034 exhibits favorable probiotic-associated characteristics, genomic safety, and TLR2-associated anti-adipogenic activity, supporting its potential as a functional probiotic or postbiotic candidate.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porcine methicillin resistant Staphylococcus aureus CC5/t002 from southwestern Nigeria lacks mec gene and carried PVL and IEC genes on prophages incorporated in the β-hemolysin region. 产自尼日利亚西南部的猪耐甲氧西林金黄色葡萄球菌CC5/t002缺乏mec基因，在β-溶血素区整合的前噬菌体上携带PVL和IEC基因。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-10-01 DOI: 10.1093/lambio/ovag085

Adewale Elijah Adejumo, Odion O Ikhimiukhor, Cheryl P Andam, Olawale Olufemi Adelowo

{"title":"Porcine methicillin resistant Staphylococcus aureus CC5/t002 from southwestern Nigeria lacks mec gene and carried PVL and IEC genes on prophages incorporated in the β-hemolysin region.","authors":"Adewale Elijah Adejumo, Odion O Ikhimiukhor, Cheryl P Andam, Olawale Olufemi Adelowo","doi":"10.1093/lambio/ovag085","DOIUrl":"https://doi.org/10.1093/lambio/ovag085","url":null,"abstract":"Despite their public health importance, data on methicillin-resistant Staphylococcus aureus lacking mec genes (MRLM) is not common in Africa. Here, we provide genome-based insights into two porcine MRLM isolates from Ogbomoso, southwestern Nigeria. Antibiotic susceptibility testing (Vitek2) and Illumina whole genome sequencing revealed closely related (ANI value of 99.997%) CC5/t002 strains showing resistance to methicillin without a corresponding mec gene, but with mutation (D105N) in gdpP associated with β-lactam resistance among MRLM isolates. Further, both isolates carried blaZ, arsBC, cadC, and the enterotoxin gene sel30 on identical mobilizable plasmids. Additional virulence genes detected in both isolates include serine proteases (splA, splB), and three copies of the staphylococcal complement inhibitor gene scn. One scn was part of the immune evasion cluster (IEC) genes on prophages which also carried PVL at the 5' end. The prophages were incorporated in the β-hemolysin region as commonly found in human-adapted isolates, and a truncated phage autolysin was inserted between the chp and sak genes at the IEC region. Our results showed a concerning presence of animal-adapted isolates with enhanced human pathogenic potentials in the food animal production chain in the study area.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal adaptation of Lactiplantibacillus plantarum by laboratory evolution does not enhance tagatose biosynthesis. 植物乳杆菌通过实验室进化的热适应性并没有促进塔格糖的生物合成。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-09-26 DOI: 10.1093/lambio/ovag084

Vikas D Trivedi, Todd C Chappell, Nikhil U Nair

{"title":"Thermal adaptation of Lactiplantibacillus plantarum by laboratory evolution does not enhance tagatose biosynthesis.","authors":"Vikas D Trivedi, Todd C Chappell, Nikhil U Nair","doi":"10.1093/lambio/ovag084","DOIUrl":"https://doi.org/10.1093/lambio/ovag084","url":null,"abstract":"D-Tagatose is recognized as a promising sugar alternative, but its biosynthesis is limited by poor thermodynamics of isomerization from D-galactose. Higher reaction temperatures favor tagatose production, but microbial whole cell conversion is limited by viability of cells at elevated temperatures. This work attempts to address this by engineering Lactiplantibacillus plantarum for growth at elevated temperatures towards development of a high-temperature galactose to tagatose isomerization biocatalytic system. Using adaptive laboratory evolution (ALE), a strain capable of growth at 43°C was isolated over 85 passages of increasing thermal stress. The adaptation required using a cycling approach, where cultures were cultured at permissive (37°C) between growth at elevated temperatures. At passage #85, the cells were able to grow at 43°C over multiple passages, indicating complete adaptation. They were also able to grow well at 37°C. Unfortunately, galactose to tagatose conversion reaction at 37, 50, and 60°C using an L-arabinose isomerase transformed strain revealed no benefit of using the evolved strain over the parental strain. Nevertheless, the ALE protocol and isolated strain may be of benefit for other applications.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149061997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0