Letters in Applied Microbiology最新文献

The effects of acidifying agents on the growth, antimicrobial resistance, and plasmid genome of Salmonella enterica serovar Infantis. 酸化剂对婴幼儿肠炎沙门氏菌生长、耐药性和质粒基因组的影响。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-03 DOI: 10.1093/lambio/ovaf115

Sohyun Cho, Taejung Chung, Gregory Zock, Adelumola Oladeinde, Michael Rothrock, Xiang Li, Jodie P Lawrence, Walid Al Hakeem, Micah Lewis

{"title":"The effects of acidifying agents on the growth, antimicrobial resistance, and plasmid genome of Salmonella enterica serovar Infantis.","authors":"Sohyun Cho, Taejung Chung, Gregory Zock, Adelumola Oladeinde, Michael Rothrock, Xiang Li, Jodie P Lawrence, Walid Al Hakeem, Micah Lewis","doi":"10.1093/lambio/ovaf115","DOIUrl":"10.1093/lambio/ovaf115","url":null,"abstract":"This study evaluated if acidifying agents used for ammonia control and pathogen reduction in poultry houses have a deleterious effect on the survival and growth of Salmonella Infantis. Changes in antimicrobial resistance (AMR) and the gene composition of the plasmid of emerging S. Infantis (pESI) were also investigated. When S. Infantis was exposed to sodium bisulfate (SBS) and acidified copper sulfate (ACS), a bacteriostatic effect on Salmonella was observed at recommended concentrations, whereas ACS at double concentration had a bactericidal effect. No difference in the maximum growth and lag phase time (P > .05) between the pESI-carrying (pESI+) and pESI-free (pESI-) strains was observed, suggesting that there was a minimal benefit or burden imposed by pESI. However, several evolved isolates of the pESI+ strains lost resistance to sulfamethoxazole and trimethoprim-sulfamethoxazole in the presence of SBS, potentially by losing the associated genes. Furthermore, applying ACS to litter microcosms post-inoculation with pESI+ strains significantly reduced the gene abundance of S. Infantis and pESI replicon (P < .05), while SBS reduced the gene abundance of pESI- strains. This study suggests that acidifiers such as ACS pose a selective pressure on pESI+ S. Infantis and broader studies are needed to investigate their efficacy for pathogen and AMR reduction in pre-harvest broiler production.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of multiple clones and extended-spectrum β-lactamase production among Klebsiella pneumoniae complex isolates from the milk of cows with different severity scores of clinical mastitis. 临床乳腺炎不同严重程度奶牛乳汁肺炎克雷伯菌复合体多克隆及广谱β-内酰胺酶产量检测

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-03 DOI: 10.1093/lambio/ovaf118

Rafaela Mastrangelo Rissetti, Carolina Lechinski de Paula, Amanda Yaeko Yamada, Amanda Maria de Jesus Bertani, Ana Beatriz Nascimento Costa, Claudio Tavares Sacchi, Karoline Rodrigues Campos, Simony Trevisan Guerra, Felipe Freitas Guimarães, Sâmea Fernandes Joaquim, Hélio Langoni, Vera Lúcia Mores Rall, Amanda Bezerra Bertolini, Patrik Júnior de Lima Paz, Thaís Spessotto Bello, Marcelo Fagali Arabe Filho, Letícia Colin Panegossi, Alec Utida Reznik, Márcio Garcia Ribeiro, Carlos Henrique Camargo

{"title":"Detection of multiple clones and extended-spectrum β-lactamase production among Klebsiella pneumoniae complex isolates from the milk of cows with different severity scores of clinical mastitis.","authors":"Rafaela Mastrangelo Rissetti, Carolina Lechinski de Paula, Amanda Yaeko Yamada, Amanda Maria de Jesus Bertani, Ana Beatriz Nascimento Costa, Claudio Tavares Sacchi, Karoline Rodrigues Campos, Simony Trevisan Guerra, Felipe Freitas Guimarães, Sâmea Fernandes Joaquim, Hélio Langoni, Vera Lúcia Mores Rall, Amanda Bezerra Bertolini, Patrik Júnior de Lima Paz, Thaís Spessotto Bello, Marcelo Fagali Arabe Filho, Letícia Colin Panegossi, Alec Utida Reznik, Márcio Garcia Ribeiro, Carlos Henrique Camargo","doi":"10.1093/lambio/ovaf118","DOIUrl":"10.1093/lambio/ovaf118","url":null,"abstract":"This study aimed to evaluate the antimicrobial susceptibility and clonal relatedness of Klebsiella pneumoniae isolates recovered from the milk of cows with mastitis in a large-scale study that clinical severity was scored. A total of 48 K. pneumoniae complex isolates were subjected to in vitro antimicrobial susceptibility tests (AST), PCR for carbapenemase-encoding genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Thirteen isolates, selected by PFGE type and AST results were subjected to whole-genome sequencing (WGS) for the identification of sequence types (STs) and the detection of antimicrobial resistance genes and virulence-encoding genes. A total of 39 different PFGE restriction profiles were identified. Thirteen different STs, including two novel STs, were identified among the 13 sequenced strains. The blaCTX-M-8, qnrE1, aadA2, cmlA4, dfrA15, sul1, tetA, and tetB genes were identified. Two isolates presented the yersiniabactin-encoding gene ybtAEPQSTUX. Klebsiella pneumoniae isolates from the milk of cows with mastitis clinically scored revealed high genetic diversity, according to both PFGE and MLST analysis, as well as harboring resistance genes commonly found in human clinical isolates.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the genomic landscape of NBAIR BtAe, a potential Bacillus thuringiensis strain against Holotrichia serrata F. 揭示苏云金芽孢杆菌抗锯齿毛枯菌潜力菌株nairbtae的基因组图谱。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-03 DOI: 10.1093/lambio/ovaf117

Lavanya Siddanahalli Manjunath, Muthuraju Ramaiah, Ramya Ramesan Syamala, Keerthi Manikyanahalli Chandrashekara, Divya Chandrashekar, Aditya Kukreti, Kolla Sreedevi, Satya Nand Sushil, Manjunatha Channappa

{"title":"Unveiling the genomic landscape of NBAIR BtAe, a potential Bacillus thuringiensis strain against Holotrichia serrata F.","authors":"Lavanya Siddanahalli Manjunath, Muthuraju Ramaiah, Ramya Ramesan Syamala, Keerthi Manikyanahalli Chandrashekara, Divya Chandrashekar, Aditya Kukreti, Kolla Sreedevi, Satya Nand Sushil, Manjunatha Channappa","doi":"10.1093/lambio/ovaf117","DOIUrl":"10.1093/lambio/ovaf117","url":null,"abstract":"Bacillus thuringiensis Berliner is a well-known biocontrol agent that produces insecticidal crystal proteins encoded by cry genes, which are effective against various insect orders. However, only a limited number of B. thuringiensis strains are known to be toxic to white grubs, a major pest of coconut, groundnut, and sugarcane that can cause up to 70%-80% yield losses. In this study, five indigenous B. thuringiensis strains, isolated from soil and Anomala elata cadavers, were screened for toxicity against second-instar Holotrichia serrata larvae, a highly destructive white grub species. Among them, the strain NBAIR BtAe exhibited the highest toxicity with an LC₅₀ of 115.36 μg mL-1. Whole-genome sequencing of NBAIR BtAe revealed a 5.67 Mb circular chromosome with 35.64% GC content. BtToxin_Digger analysis identified a novel cry gene with 39.57% similarity to cry21Aa2, along with other virulence genes including zwa6, zwa5A, chitinase C, inhA1, inhA2, bmp1, spp1Aa1, enhancin, and tpp80Ab1-like. These genes were validated through PCR. Additionally, genes encoding secondary metabolites such as lanthipeptides, paenilamicin, petrobactin, bacillibactin, and fengycin were detected. The presence of diverse pesticidal and antimicrobial genes highlights the potential of NBAIR BtAe as a promising candidate for bioinsecticide development targeting H. serrata in integrated pest management programs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and biotechnological potential of actinobacteria from the Krem Dam Cave, Meghalaya, India. 印度梅加拉亚邦Krem坝洞放线菌的多样性和生物技术潜力。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-03 DOI: 10.1093/lambio/ovaf120

Debulman Syiemiong, Jintu Rabha, Ramasamy Vijayakumar, Thomas Arbenz, Pynshailang Syiemiong, Kevin Matthew Lamare, Chalcedony Wahlang, Rangehbok Lyngkhoi

{"title":"Diversity and biotechnological potential of actinobacteria from the Krem Dam Cave, Meghalaya, India.","authors":"Debulman Syiemiong, Jintu Rabha, Ramasamy Vijayakumar, Thomas Arbenz, Pynshailang Syiemiong, Kevin Matthew Lamare, Chalcedony Wahlang, Rangehbok Lyngkhoi","doi":"10.1093/lambio/ovaf120","DOIUrl":"10.1093/lambio/ovaf120","url":null,"abstract":"This study aimed to explore the cultivable actinobacterial diversity in Krem Dam cave, Meghalaya, India, and to evaluate their biotechnological potential through antimicrobial activity, plant growth-promoting traits, and metabolic pathway prediction. Sediment samples were collected from five locations within the cave, pretreated, and cultured on selective media to isolate actinobacteria. Isolates were characterized morphologically, physiologically, and chemotaxonomically, followed by 16S rDNA sequencing for molecular identification. The PAPRICA pipeline was used to predict metabolic pathways from 16S rDNA sequences. Antimicrobial activity was assessed against Gram-positive, Gram-negative bacteria, and Candida species using cross-streak and agar-well diffusion methods, while biosynthetic gene clusters (PKS-I, PKS-II, and NRPS) were screened via polymerase chain reaction (PCR). Plant growth-promoting (PGP) traits, including IAA production, phosphate solubilization, siderophore activity, and nitrogen fixation were evaluated, along with antagonism against phytopathogens and seedling vigor in tomato. Forty-eight isolates were identified, predominantly Streptomyces thermocarboxydus-related strains, with one Amycolatopsis species. Seventy-seven percent harbored at least one biosynthetic gene cluster, and significant antimicrobial activity was observed, particularly against Gram-positive bacteria. Several isolates exhibited multiple PGP traits, and two (KD-21, KD-29) enhanced tomato seedling vigor. The study concludes that Krem Dam cave harbors diverse, bioactive actinobacteria with promising applications in pharmaceuticals and sustainable agriculture, warranting further metabolomic and genomic investigations.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequencing of Mycoplasma genitalium genomes using a tiling amplicon method on the Nanopore MinION. 利用纳米孔MinION上的平铺扩增子方法对生殖支原体基因组进行测序。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-03 DOI: 10.1093/lambio/ovaf119

Teck-Phui Chua, Jennifer A Danielewski, Jose L Huaman, Erica L Plummer, Catriona S Bradshaw, Lenka A Vodstrcil, Dorothy A Machalek, Suzanne M Garland, Gerald L Murray

{"title":"Sequencing of Mycoplasma genitalium genomes using a tiling amplicon method on the Nanopore MinION.","authors":"Teck-Phui Chua, Jennifer A Danielewski, Jose L Huaman, Erica L Plummer, Catriona S Bradshaw, Lenka A Vodstrcil, Dorothy A Machalek, Suzanne M Garland, Gerald L Murray","doi":"10.1093/lambio/ovaf119","DOIUrl":"10.1093/lambio/ovaf119","url":null,"abstract":"Mycoplasma genitalium is challenging to work with and new methods are needed to study this bacterium directly in clinical samples. This study designed and validated a proof-of-concept polymerase chain reaction (PCR)-based 'tiling' methodology to sequence M. genitalium genomes. Primers were designed to produce 2.5 kb amplicons covering the 580 kb genome with a minimum overlap of 100 bp. Analysis was performed using the laboratory strain G37 and a clinical isolate. Amplicons were sequenced on the Oxford Nanopore MinION using ligation sequencing. Reads were mapped to a reference to produce a consensus genome. A total of 262 primer pairs were designed and amplification was successful for 99.5% (261/262) of 2.5 kb amplicons, with G37 genome coverage of 99.5% (mean read depth, 1973X). Using larger 5kb amplicons, amplification was successful for 92.4% (121/131) of primer pairs, with a coverage of 92.2% (mean read depth, 223X). When validated on a clinical isolate, 98.3% coverage was achieved (read depth, 443X). In conclusion, this study developed a PCR-based tiling approach to whole genome sequencing of M. genitalium by designing and validating a set of 262 primer pairs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic signatures of beta-lactam resistance and reversion in Staphylococcus aureus from bovine mastitis. 牛乳腺炎金黄色葡萄球菌β -内酰胺耐药和逆转的基因组特征。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-03 DOI: 10.1093/lambio/ovaf107

Bigya Dhital, Ying-Tsong Chen, Sushil Paudyal, Rameshwor Pudasaini, Yi-Tzu Chen, Hsin-I Chiang

{"title":"Genomic signatures of beta-lactam resistance and reversion in Staphylococcus aureus from bovine mastitis.","authors":"Bigya Dhital, Ying-Tsong Chen, Sushil Paudyal, Rameshwor Pudasaini, Yi-Tzu Chen, Hsin-I Chiang","doi":"10.1093/lambio/ovaf107","DOIUrl":"10.1093/lambio/ovaf107","url":null,"abstract":"This study uses whole genome sequencing (WGS) to identify beta-lactam resistance-associated mutations in in vitro selected Staphylococcus aureus subsp. aureus ATCC 25923 strains, and correlates the findings with isolates collected from mastitis-infected dairy cows. Resistance was induced in a susceptible strain of S. aureus subsp. aureus ATCC 25923 through serial in vitro exposure to ampicillin sodium salt, cefapirin, cefuroxime sodium salt, and cefquinome. The resulting resistant isolates exhibited thousands of fold increases in minimum inhibitory concentrations (MICs), compared to the susceptible strain. In the absence of antimicrobial selection pressure, the MIC decreased by up to 32-fold, indicating a significant restoration of antimicrobial susceptibility. WGS identified resistance-associated mutations in the penicillin-binding proteins, ABC transporter ATP-binding protein, cell wall-active antibiotics response protein, cyclic-di-AMP phosphodiesterase, and two-component system sensor histidine kinase. Additionally, these mutations were investigated in the S. aureus isolates collected from mastitis-infected dairy cows. These isolates shared the same resistance-associated mutations as in vitro-selected strains. These findings demonstrate that resistance mutations identified through in vitro selection are also present in clinical isolates, highlighting the clinical applicability of in vitro selection for understanding antimicrobial resistance in mastitis-associated S. aureus.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Standard pH: Unmasking the Importance of pH-Mimicking Conditions for reliability of Enzyme Activity in Vulvovaginal Candida albicans Isolates. 超出标准pH值：揭示pH模拟条件对外阴阴道白色念珠菌分离物酶活性可靠性的重要性。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-10-01 DOI: 10.1093/lambio/ovaf122

Hasti Nouraei, Darya Najafi Tirehshabankareh, Keyvan Pakshir

{"title":"Beyond Standard pH: Unmasking the Importance of pH-Mimicking Conditions for reliability of Enzyme Activity in Vulvovaginal Candida albicans Isolates.","authors":"Hasti Nouraei, Darya Najafi Tirehshabankareh, Keyvan Pakshir","doi":"10.1093/lambio/ovaf122","DOIUrl":"https://doi.org/10.1093/lambio/ovaf122","url":null,"abstract":"Enzymatic activity potency in Candida albicans is depends on different pH levels. This study investigates the importance of matching in vitro pH conditions to those encountered at infection sites. A total of 20 vulvovaginitis Candida albicans isolates were investigated for phospholipase, proteinase, and esterase activities. The activities were measured at three pH levels (4, 5, and 7) representing for the healthy vaginal range, potential infection condition, and a standard in vitro reference point, respectively. Specific media were used to assess enzyme activity. Phospholipase and proteinase activity were significantly higher at acidic pH compared to neutral pH. In contrast, esterase activity showed a slight increase at neutral pH. Analysis revealed significant differences in enzyme activity between pH 5 and 7, highlighting the importance of using pH-relevant conditions for studying Candida virulence. This study reveals that the acidic vaginal pH characteristic of Candida albicans infections significantly enhances the activity of its key damaging enzymes. This highlights the limitations of using a standard pH protocol for enzyme activity analysis. By employing pH-mimicking conditions, future research can unlock a deeper understanding the actual C. albicans virulence that might occur in site of infection. Data analysis should consider beyond this factor.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Specific Detection of Mycoplasma pneumoniae Using Pyrococcus furiosus Argonaute Combined with Recombinase Polymerase Amplification. Argonaute火焰球菌联合重组酶聚合酶扩增快速特异检测肺炎支原体

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-09-26 DOI: 10.1093/lambio/ovaf121

Ping Yao, Jian Xu, Jingyi Jiang, Li Gong, Xin Dong, Bowen Tu, Jinyi Jiang, XuJian Mao, Fengming Wang, Qiong Li

引用次数: 0

Targeting kidney inflammation in colitis: the role of probiotics, paraprobiotics, and autophagy. 针对结肠炎的肾脏炎症：益生菌、副益生菌和自噬的作用。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-09-04 DOI: 10.1093/lambio/ovaf113

Fatemeh Haririzadeh Jouriani, Mahnaz Torfeh, Fatemeh Ashrafian, Niloofar Rezaie, Shadi Aghamohammad, Mahdi Rohani

引用次数: 0

Lactic acid bacteria and related strains isolated from Hibiscus sabdariffa L. calyces: from isolation to evaluation of technological potential. 芙蓉花萼中乳酸菌及其相关菌株：从分离到技术潜力评价。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2025-09-04 DOI: 10.1093/lambio/ovaf114

Migdalia Sarahy Navidad-Murrieta, Adela Y Bueno-Durán, Josué Alegría-Gómez, Victor Ruíz-Valdiviezo, Victor M Zamora-Gasga, Adrián Hernández-Mendoza, Jorge A Sánchez-Burgos, Manuel Iván Girón-Pérez, Sonia G Sáyago-Ayerdi

{"title":"Lactic acid bacteria and related strains isolated from Hibiscus sabdariffa L. calyces: from isolation to evaluation of technological potential.","authors":"Migdalia Sarahy Navidad-Murrieta, Adela Y Bueno-Durán, Josué Alegría-Gómez, Victor Ruíz-Valdiviezo, Victor M Zamora-Gasga, Adrián Hernández-Mendoza, Jorge A Sánchez-Burgos, Manuel Iván Girón-Pérez, Sonia G Sáyago-Ayerdi","doi":"10.1093/lambio/ovaf114","DOIUrl":"10.1093/lambio/ovaf114","url":null,"abstract":"The isolation and identification of native lactic acid bacteria (LAB) from plant matrix and flowers, which are potential sources of novel strains for fermented food development. Accordingly, this study aimed to determine the presence and bacterial diversity in Hibiscus sabdariffa L. calyces, and to characterize their technological properties for potential food application. Nine isolates were identified by 16S rRNA gene sequencing, belonging to the genera Leuconostoc (JC1 and JC2), Bacillus (JC3), Limosilactobacillus (JC4), Lentilactobacillus (JC5 and JC6), Enterococcus (JC7 and JC8), and Lactococcus (JC9). Cell growth kinetics, lactic acid production, and pH reduction were used to characterize the isolated bacteria. Additionally, the technological potential of the isolates was evaluated by assessing their survival under refrigeration (4°C), freezing (-20°C), and pasteurization (both fast and slow) conditions. Susceptibility/resistance to 12 antibiotics was also determined. Results showed that isolate JC3 (Bacillus velezensis) exhibited outstanding growth parameters, tolerance to pasteurization thermal conditions (63°C/30 min and 72°C/15 s) and susceptibility to the 12 tested antibiotics. These results highlight the potential of Hibiscus calyces as a valuable source of diverse native LAB and related strains with promising technological traits. Further studies on the functionality and safety of these bacteria are required to confirm their probiotic potential.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0