Letters in Applied Microbiology最新文献

Role of Fe and Mn in organo-mineral-microbe interactions: evidence of carbon stabilization and transformation of organic matter leading to carbon greenhouse gas emissions.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-21 DOI: 10.1093/lambio/ovaf044

Andrew Elohim Laloo, Abhishek Gupta, Valeria Verrone, Rama Kant Dubey

{"title":"Role of Fe and Mn in organo-mineral-microbe interactions: evidence of carbon stabilization and transformation of organic matter leading to carbon greenhouse gas emissions.","authors":"Andrew Elohim Laloo, Abhishek Gupta, Valeria Verrone, Rama Kant Dubey","doi":"10.1093/lambio/ovaf044","DOIUrl":"https://doi.org/10.1093/lambio/ovaf044","url":null,"abstract":"Up to 90% of organic matter (OM) in soils and sediments are stabilized and protected against microbial decomposition through organo-mineral interactions, formation of soil aggregates, pH and oxygen availability. In soils and sediment systems OM is associated with mineral constituents promoting carbon persistence and sequestration of which iron (Fe) and manganese (Mn) are crucial components. Under anoxic condition, microbes couple the decomposition of OM to the oxidative/reductive transformation of Fe/Mn minerals leading to carbon greenhouse gas (C-GHG) emissions (i.e., CH4 and CO2). Although these organo-mineral-microbe interactions have been observed for decades, the bio-geochemical mechanisms governing the switch from OM stability toward OM degradation are not fully understood. Interest in this field have been growing steadily given the interest in global warming caused by OM decomposition leading to C-GHG emissions. This review emphasizes the dual role of Fe/Mn minerals in both OM stability and decomposition. Additionally, we synthesize the conceptual understanding of how Fe/Mn minerals govern OM dynamics and the resultant C-GHG emissions via microbial-mediated carbon transformation. We highlight the need for interdisciplinary research to better understand organo-Fe/Mn mineral-microbial interactions to develop management handles for climate mitigation strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human gut microbiome and sleep across adulthood: associations and therapeutic potential.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-20 DOI: 10.1093/lambio/ovaf043

Jacob Cavon, Melissa Basso, Kathrin Cohen Kadosh, Sean M Gibbons

引用次数: 0

Enhancing Blueberries Safety: Antimicrobial Effects of Alkyltrimethylammonium Bromide Against Foodborne Pathogens.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-19 DOI: 10.1093/lambio/ovaf042

Syed Ammar Hussain, Majher I Sarker, Tony Z Jin

引用次数: 0

Characterization, antifungal activity and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-18 DOI: 10.1093/lambio/ovaf040

Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior

{"title":"Characterization, antifungal activity and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains.","authors":"Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior","doi":"10.1093/lambio/ovaf040","DOIUrl":"https://doi.org/10.1093/lambio/ovaf040","url":null,"abstract":"This study aimed to characterize Melissa officinalis essential oil (EOMO) from a region in Northeast Brazil and evaluate its antifungal activity against Candida and Cryptococcus neoformans, by analyzing its action mechanism. EOMO was characterized using gas chromatography-mass spectrometry and the main chemical compounds were Geranial (14.10%) and (Z)-Nerolidol (17.75%). The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) against strains of Candida albicans, Candida parapsilosis, Candida krusei, Candida auris (MIC raging from 256 to 26.7 µg ml-1) and C. neoformans (MIC ranging from 64 to 32 µg ml-1). Flow cytometry and comet assays were employed to investigate EOMO's mechanism of action, which might be related to an increase in the production of reactive oxygen species and damage to fungal DNA. The chemical composition of EOMO from Northeast Brazil showed a higher content of (Z)-Nerolidol and has significant antifungal potential.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of multiplex PCR for detection of foodborne pathogens in fresh produce.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-17 DOI: 10.1093/lambio/ovaf038

Ian Moppert, Eiseul Kim, Seung-Min Yang, Si Hong Park

{"title":"Development of multiplex PCR for detection of foodborne pathogens in fresh produce.","authors":"Ian Moppert, Eiseul Kim, Seung-Min Yang, Si Hong Park","doi":"10.1093/lambio/ovaf038","DOIUrl":"https://doi.org/10.1093/lambio/ovaf038","url":null,"abstract":"Foodborne pathogens present a significant public health concern where fresh produce is a key agricultural product. Rapid and sensitive detection methods are essential to ensure the safety of such produce. This study aimed to develop and optimize a modified multiplex PCR (mPCR) assay, which incorporates enhancements to conventional PCR, for the simultaneous detection of Escherichia coli, Salmonella, and Listeria monocytogenes in fresh produce. The specificity of each primer pair was validated using 15 strains, confirming 100% accurate detection of pathogenic strains without cross-reactivity. Since no false positives were observed, the assay demonstrated 100% precision, highlighting its reliability in distinguishing target pathogens. The sensitivity of the mPCR assay was demonstrated through serial dilutions, detecting Salmonella down to 10 fg µl⁻1, L. monocytogenes to 100 fg µl⁻1, and E. coli to 1 pg µl⁻1. The mPCR assay was then successfully applied to romaine lettuce and kale, demonstrating its effectiveness in detecting pathogens in mixed samples inoculated at varying concentrations (109 to 101 CFU ml⁻1). Kale exhibited greater sensitivity, detecting pathogens at lower levels, while romaine lettuce also provided consistent detection. This study highlights the potential of mPCR for enhancing food safety by providing rapid and sensitive pathogen detection in fresh produce.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Vibrio cholerae from the Jukskei River in Johannesburg South Africa.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-12 DOI: 10.1093/lambio/ovaf036

Luyanda Mkhize, Musa Marimani, Sanelisiwe Thinasonke Duze

{"title":"Characterization of Vibrio cholerae from the Jukskei River in Johannesburg South Africa.","authors":"Luyanda Mkhize, Musa Marimani, Sanelisiwe Thinasonke Duze","doi":"10.1093/lambio/ovaf036","DOIUrl":"https://doi.org/10.1093/lambio/ovaf036","url":null,"abstract":"The current study aimed to isolate and characterize Vibrio cholerae (V. cholerae) isolated from the Jukskei River, one of the largest Rivers in Johannesburg, South Africa. Water samples collected from the Jukskei River were subjected to culture-based methods for the detection and isolation of V. cholerae. Twenty-four V. cholerae were isolated, confirmed using real-time PCR, and sequenced using the MInION portable nanopore-sequencing device. Reference-based genome assemblies were constructed from the raw reads using the EPI2ME software followed by bioinformatics analysis using the Centre for Genomic Epidemiology website. All the V. cholerae isolates isolated from the Jukskei River were classified as non-O1/ non-O139 and none of the isolates harbored the cholera toxin gene, ctxA. All 24 V. cholerae isolates belonged to sequence type 741, virulent genes including toxR, vspD, als, hlyA, makA, and rtxA as well as the Vibrio pathogenicity island 2 were detected amongst the isolates. Antimicrobial resistance genes (parC, varG, and gyrA) were detected in 83% of isolates. Although V. cholerae non-O1/non-O139 are not associated with epidemic cholera they can still cause mild to life-threatening illnesses. Therefore, increased surveillance should be considered to better understand the public health risks to the local community.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free supernatant of Clostridium leptum inhibits breast cancer cell proliferation.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-12 DOI: 10.1093/lambio/ovaf037

Qingling Wang, Huan Zhao, Huizhe Ding, Hao Zhang, Jizhou Zhang, Liqin Li, Bing Han, Guoyin Kai

{"title":"Cell-free supernatant of Clostridium leptum inhibits breast cancer cell proliferation.","authors":"Qingling Wang, Huan Zhao, Huizhe Ding, Hao Zhang, Jizhou Zhang, Liqin Li, Bing Han, Guoyin Kai","doi":"10.1093/lambio/ovaf037","DOIUrl":"https://doi.org/10.1093/lambio/ovaf037","url":null,"abstract":"Breast cancer has emerged as the leading cause of global cancer incidence, surpassing lung cancer. Accumulating evidence suggests that probiotics exhibit inhibitory effect on breast cancer progression, highlighting the need to identify gut flora-derived probiotics with potential anti-breast cancer properties. Here, we investigated the effect of the cell-free supernatant of C. leptum (ClCFS) on breast cancer cells by MTT assay. Untargeted metabolomics analysis was employed to characterize metabolite alterations in ClCFS. Furthermore, the core targets were predicted by the protein-protein interaction network and the signaling pathways were enriched by the Kyoto Encyclopedia of Genes and Genomes analysis. Our findings demonstrated that ClCFS inhibited the proliferation of breast cancer cells and that the main metabolite of ClCFS might be acetylcarnitine. Utilizing network pharmacological analysis, we identified apoptosis-related signaling pathways as the principal mechanisms underlying ClCFS activity. Furthermore, five core targets of STAT3, IL-1β, BCL2, CASP3, and ESR1 were identified. This study elucidates the main bioactive constituent and the potential targets of ClCFS against breast cancer. It provides a new understanding of the pharmacological activity of ClCFS in breast cancer treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelic exchange technologies for Clostridioides difficile research and their application in toxin studies. 用于艰难梭菌研究的等位基因交换技术及其在毒素研究中的应用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-11 DOI: 10.1093/lambio/ovaf034

Terry W Bilverstone

引用次数: 0

Designing a method to test the efficacy of different sanitizers for use in rubber footbaths as applied to dairy manufacturing.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-11 DOI: 10.1093/lambio/ovaf035

D Lindsay, V Thompson, M Harrigan

引用次数: 0

Modelling the effect of peracetic acid / hydrogen peroxyde solution concentration and temperature treatment on Bacillus cereus spores inactivation.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-08 DOI: 10.1093/lambio/ovaf033

Leguérinel Ivan, Mathot Anne-Gabrielle, Camarero Adrienne, Coroller Louis

{"title":"Modelling the effect of peracetic acid / hydrogen peroxyde solution concentration and temperature treatment on Bacillus cereus spores inactivation.","authors":"Leguérinel Ivan, Mathot Anne-Gabrielle, Camarero Adrienne, Coroller Louis","doi":"10.1093/lambio/ovaf033","DOIUrl":"https://doi.org/10.1093/lambio/ovaf033","url":null,"abstract":"Cleaning and sanitizing processing equipment are essential for achieving the hygiene standards required in food processing. The term \"sanitizing\" refers to the inactivation of micro-organisms by disinfectants on previously cleaned surfaces and materials. In the food industry, commercial solutions of peracetic acid (PAA) and hydrogen peroxide (HP) show bactericidal and sporicidal activity at room temperature, and are used as disinfectants for food surfaces, fruit and vegetables, or to sanitize water. The aim of this study was to quantify the impact of commercial solutions of peracetic acid and hydrogen peroxide on Bacillus cereus endospores as a function of concentration and temperature, in order to optimize its use. Inactivation kinetics were carried at 5 PAA/HP solution concentration levels and three temperature levels. Weibull model fits on concave inactivation kinetics quantified Bacillus cereus spore resistances. A Bigelow-type model quantified the effects of concentration and temperature on spore resistance Weibull model parameters. The parameters of the Bigelow type model enable to optimize disinfection treatments, reducing the concentration of PAA / HP solutions used by increasing the treatment temperature, while ensuring the same level of disinfectant efficacy against bacterial spores.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0