Letters in Applied Microbiology最新文献_第10页

Xpert MTB/RIF Ultra as a diagnostic tool for pleural tuberculosis: a study at Indian tertiary care centre. 作为胸膜结核诊断工具的 Xpert MTB/RIF Ultra：印度三级医疗中心的一项研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae102

Sumedha Sharma, Prabhdeep Kaur, Ashutosh N Aggarwal, Khushpreet Kaur, Rakesh Yadav, Sunil Sethi, Indu Verma

{"title":"Xpert MTB/RIF Ultra as a diagnostic tool for pleural tuberculosis: a study at Indian tertiary care centre.","authors":"Sumedha Sharma, Prabhdeep Kaur, Ashutosh N Aggarwal, Khushpreet Kaur, Rakesh Yadav, Sunil Sethi, Indu Verma","doi":"10.1093/lambio/ovae102","DOIUrl":"10.1093/lambio/ovae102","url":null,"abstract":"Despite the advent of Xpert MTB/RIF, pleural tuberculosis (TB) diagnosis in pleural fluid is still difficult. Hence, we assessed the diagnostic efficacy of its advanced version, Xpert MTB/RIF Ultra, for pleural TB diagnosis using pleural fluid as a sample. Tuberculosis pleuritis (TBP) suspects (n = 261) were enrolled in the study, of which 29 were excluded. The remaining patients (n = 232) were categorized into definite TBP (n = 31), probable TBP (n = 28), and non-TB controls (n = 173) based on the composite reference standard consisting of smear, culture, histopathology, and Xpert MTB/RIF as well as follow-up/clinical response to anti-TB therapy. Among the TBP suspects, 59 were diagnosed as TBP patients. The sensitivity of Xpert MTB/RIF Ultra (52.5%) using pleural fluid for TBP diagnosis was higher than sensitivity obtained with smear (22.4%), culture (17.6%), and Xpert MTB/RIF (25%) alone, carried out using either pleural fluid or pleural biopsy or both the samples. In cases of probable TBP, where none of the laboratory tests were positive, Xpert MTB/RIF Ultra use led to an increased diagnostic percentage of definite TBP from 52.5% to 69.4%. Overall, Xpert MTB/RIF Ultra showed promising results for a definitive diagnosis of TBP in pleural fluid samples.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The application of ozone within the food industry, mode of action, current and future applications, and regulatory compliance. 臭氧在食品工业中的应用、作用方式、当前和未来的应用以及监管合规性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae101

Zak Hamid, Ben K Meyrick, Joshua Macleod, Emily A Heath, James Blaxland

{"title":"The application of ozone within the food industry, mode of action, current and future applications, and regulatory compliance.","authors":"Zak Hamid, Ben K Meyrick, Joshua Macleod, Emily A Heath, James Blaxland","doi":"10.1093/lambio/ovae101","DOIUrl":"10.1093/lambio/ovae101","url":null,"abstract":"The food industry faces numerous challenges today, with the prevention and reduction of microbial contamination being a critical focus. While traditional chemical-based methods are effective and widely used, rising energy costs, the development of microbial tolerances, and growing awareness of the ecological impact of chemical biocides have renewed interest in novel biocides. Ozone, in both its gaseous and aqueous forms, is recognized as a potent disinfectant against bacteria, viruses, and fungi due to its high oxidation potential. Our review highlights several studies on the applications of ozone within the food industry, including its use for surface and aerosol disinfection and its capacity to reduce viable Listeria monocytogenes, a pertinent foodborne pathogen harbouring environmental and biocide stress tolerances and biofilm former. We also explore the use of ozone in food treatment and preservation, specifically on blueberries, apples, carrots, cabbage, and cherry tomatoes. While ozone is an effective disinfectant, it is important to consider material incompatibility, and the risks associated with prolonged human exposure to high concentrations. Nevertheless, for certain applications, ozone proves to be an efficacious and valuable alternative or complementary method for microbial control. Compliance with the biocide products regulation will require ozone device manufacturers to produce proven efficacy and safety data in line with British standards based on European standards (BS EN), and researchers to propose adaptations to account for ozone's unique properties.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of probiotic Bacillus sp. dietary supplementation on pancreatic and intestinal activities in seabream Sparus aurata. 膳食中添加益生菌对鲷鱼胰腺和肠道活动的影响

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae104

Şükrü Yıldırım, Cüneyt Suzer, Kürşat Fırat, Şahin Saka, Müge Hekimoğlu, Deniz Çoban, Ali Yıldırım Korkut, İbrahim Köse, Onurkan Antepli, Alize Gökvardar, Fatih Perçin

{"title":"Impact of probiotic Bacillus sp. dietary supplementation on pancreatic and intestinal activities in seabream Sparus aurata.","authors":"Şükrü Yıldırım, Cüneyt Suzer, Kürşat Fırat, Şahin Saka, Müge Hekimoğlu, Deniz Çoban, Ali Yıldırım Korkut, İbrahim Köse, Onurkan Antepli, Alize Gökvardar, Fatih Perçin","doi":"10.1093/lambio/ovae104","DOIUrl":"10.1093/lambio/ovae104","url":null,"abstract":"The main objective of this study was to investigate the effects of commercial probiotic Bacillus sp. supplementation on seabream Sparus aurata larviculture under culture conditions. In this context, Bacillus was supplemented via rotifer feeding and water and its effects on pancreatic and intestinal enzyme activities as well as aquaculture parameters were evaluated during early life development. In the experimental group, as probiotic three Bacillus sp. spores were introduced via rotifer and larval culture tanks, while the larvae in control group did not feed any probiotic supplementation. At the end of the experiment on 40 days after hatching, the probiotic-supplemented group exhibited better growth performance and there were statistically differences in between groups of probiotic-treated and control regarding growth parameters (P < 0.01), despite insignificant survival rate (P > 0.05). In terms of enzymatic expressions, S. aurata larvae receiving probiotic supplementation through rotifers demonstrated noteworthy (P < 0.05) enhancements in specific activities of pancreatic and intestinal enzymes, except for amylase (P > 0.05), when compared to the control group. It is concluded that the administration of Bacillus sp. as probiotic bacteria through rotifer supplementation and water intake demonstrates significant positive impacts on both growth parameters and specific activities of main pancreatic and intestinal enzymes of seabream larvae.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermosensitive multivesicular liposomal hydrogel: a potential platform for loco-regional drug delivery in the treatment of osteomyelitis caused by antibiotic-resistant biofilm-forming bacteria. 热敏多囊脂质体水凝胶：治疗抗生素生物膜形成细菌引起的骨髓炎的局部区域给药潜在平台。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae092

Melody Vatankhah, Arash Mahboubi, Reyhaneh Varshochian, Azadeh Haeri, Hamidreza Houri, Zahra Abbasian, Simin Dadashzadeh

{"title":"Thermosensitive multivesicular liposomal hydrogel: a potential platform for loco-regional drug delivery in the treatment of osteomyelitis caused by antibiotic-resistant biofilm-forming bacteria.","authors":"Melody Vatankhah, Arash Mahboubi, Reyhaneh Varshochian, Azadeh Haeri, Hamidreza Houri, Zahra Abbasian, Simin Dadashzadeh","doi":"10.1093/lambio/ovae092","DOIUrl":"10.1093/lambio/ovae092","url":null,"abstract":"Biofilm-mediated osteomyelitis presents significant therapeutic challenges. Given the limitations of existing osteomyelitis treatment approaches, there is a distinct need to develop a localized drug delivery system that is biocompatible, biodegradable, and capable of controlled antibiotic release. Multivesicular liposomes (MVLs), characterized by their non-concentric vesicular structure, distinct composition, and enhanced stability, serve as the system for a robust sustained-release drug delivery platform. In this study, various hydrogel formulations composed of poloxamer 407 and other hydrogels, incorporating vancomycin hydrochloride (VAN HL)-loaded MVLs (VAN HL-MVLs), were prepared and evaluated. The optimized VAN HL-MVL sol-gel system, consisting of poloxamer 407 and hyaluronic acid, successfully maintained drug release for up to 3 weeks and exhibited shear-thinning behavior at 37°C. While complete drug release from MVLs alone took place in 312 h, the hydrogel formulation extended this release to 504 h. The released drug effectively inhibited the Staphylococcus aureus biofilms growth within 24 h and methicillin-resistant S. aureus biofilms within 72 h. It also eradicated preformed biofilms of S. aureus and methicillin-resistant S. aureus in 96 and 120 h, respectively. This injectable in situ gel system incorporating VAN HL-MVLs holds potential as an alternative to undergoing multiple surgeries for osteomyelitis treatment and warrants further studies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-light fluorescence as a confirmation method for presumptive Legionella colonies isolated from water samples. 用紫外荧光法确认从水样中分离出的推定军团菌菌落。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae094

Paul Badoux, Adrie Atsma, Esther van Harmelen-Vrins, Sjoerd Euser

{"title":"UV-light fluorescence as a confirmation method for presumptive Legionella colonies isolated from water samples.","authors":"Paul Badoux, Adrie Atsma, Esther van Harmelen-Vrins, Sjoerd Euser","doi":"10.1093/lambio/ovae094","DOIUrl":"10.1093/lambio/ovae094","url":null,"abstract":"Legionnaires' disease (LD) is a severe and potentially fatal form of bacterial pneumonia caused by Legionella spp. We evaluated the use of UV-light for detecting Legionella non-pneumophila in water samples according to the NEN-EN-ISO 11731:2017 methodology (reference method) in a collaborative effort involving 10 laboratories. First, a test panel was constructed of 298 strains: 157 Legionella strains and 141 non-Legionella strains were cultured on buffered charcoal yeast extract (BCYE)-medium and confirmed according to ISO 11731: 2017 (cultured on BCYE agar plates with and without l-cysteine), and by matrix-assisted laser desorption-ionization time of flight or next generation sequencing. All strains were additionally exposed to an UV-light to assess if they showed a bright blue fluorescence effect (UV-positive) or not (UV-negative). Second, in an interlaboratory study, 10 laboratories analyzed a blinded set of 16 Legionella strains and 8 non-Legionella strains using both methods. The test panel analyses showed 100% accordance between the UV-light and reference method. In addition, the interlaboratory study results showed full agreement between both methods. Our results support the implementation of UV-light detection to confirm Legionella presumptive colonies during analyses of water samples according to the NEN-EN-ISO 11731:2017 methodology. Implementation of UV-light confirmation could reduce workload, time-to-result and costs for the analyses of water samples for the presence of Legionella.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction of: The gene coding for nigrescin produced by Prevotella nigrescens ATCC 25261. 撤回：黑云纹前菌 ATCC 25261 产生的黑云素编码基因。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae093

引用次数: 0

Editor's note on 'Preventing contamination of PCR-based multiplex assays including the use of a dedicated biosafety cabinet'. 关于 "防止基于 PCR 的多重检测污染，包括使用专用生物安全柜 "的编者按。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae096

引用次数: 0

Antibacterial effect of Cu2O/TiO2 visible-light photocatalytic composite on Xanthomonas campestris. Cu2O/TiO2 可见光光催化复合材料对野菜黄单胞菌的抗菌作用

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae087

Ying Jiang, Shiyu Zhou, Liuhong Chen, Yuning Huo, Guozheng Huang, Jianguo Cao, Xiling Dai

{"title":"Antibacterial effect of Cu2O/TiO2 visible-light photocatalytic composite on Xanthomonas campestris.","authors":"Ying Jiang, Shiyu Zhou, Liuhong Chen, Yuning Huo, Guozheng Huang, Jianguo Cao, Xiling Dai","doi":"10.1093/lambio/ovae087","DOIUrl":"10.1093/lambio/ovae087","url":null,"abstract":"In this study, a Cu2O/TiO2 (CuTi) visible-light photocatalytic composite was employed for the treatment of Xanthomonas campestris and X. campestris-infected Brassica napus seedlings. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against X. campestris were determined to be 8 and 32 μg ml-1, respectively. Transmission electron microscopy analysis demonstrated a direct correlation between the extent of bacterial cell damage and the concentration of CuTi. Noteworthily, a bactericidal rate of 100% was achieved at a concentration of 150 μg ml-1 over a treatment duration of 120 min. Moreover, alterations in active oxidants and antioxidants, including reactive oxygen species, glutathione reductase, superoxide dismutase, peroxidase, and catalase within the bacterial cells, were examined to elucidate the underlying mechanism of inhibition by the CuTi. The B. napus infected by X. campestris was treated with CuTi, and the efficacy was validated through determination of plant resistance indexes. The combined data confirmed that the CuTi is characterized by a low dose, fast onset, good effect, and higher safety for killing X. campestris, and it is expected to be developed as an antimicrobial agent for vegetables.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of colistin-resistant carbapenemase producing Klebsiella pneumoniae in a river receiving wastewater treatment plant effluent. 接收污水处理厂污水的河流中产生耐大肠菌素碳青霉烯酶肺炎克雷伯氏菌的特征。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae090

Pragyan Paramita Swain, Saubhagini Sahoo, Birasen Behera, Dibyajyoti Uttameswar Behera, Enketeswara Subudhi, Rajesh Kumar Sahoo

{"title":"Characterization of colistin-resistant carbapenemase producing Klebsiella pneumoniae in a river receiving wastewater treatment plant effluent.","authors":"Pragyan Paramita Swain, Saubhagini Sahoo, Birasen Behera, Dibyajyoti Uttameswar Behera, Enketeswara Subudhi, Rajesh Kumar Sahoo","doi":"10.1093/lambio/ovae090","DOIUrl":"10.1093/lambio/ovae090","url":null,"abstract":"Genes conferring antibiotic resistance phenotype, particularly to last resort antibiotics, pose a significant concern globally. Wastewater treatment plant (WWTP) effluent substantially contributes to antibiotic resistance in receiving rivers, threatening human health. Globally, colistin- and carbapenem-resistant Klebsiella pneumoniae infections cause high morbidity and mortality. We investigated colistin-resistant carbapenemase-producing K. pneumoniae (Co-CRKP) isolates in Kathajodi river receiving WWTP effluent, their resistance genes, and pathogenic potential. Four isolates (Co-CRKP-7, Co-CRKP-8, Co-CRKP-10, and Co-CRKP-15) exhibited extensively drug-resistant (XDR) phenotype, harbouring blaTEM-1, blaCTX-M-15, blaNDM-5, and blaOXA-48 genes. Colistin resistance was attributed to mutations in the pmrA and pmrB genes. Virulence genes (fimH, mrkD, entB, iucA, iutA, and irp1), capsular serotypes (K1, K2) and biofilm formation in the isolates explicated their pathogenicity. Furthermore, Inc plasmid replicons (Y, FrepB, P, K/B, L/M, N, FIA, A/C, and FIB) indicated the dissemination potential of the resistance genes in Co-CRKP isolates. The multi-locus sequence typing showed that Co-CRKP-7 and Co-CRKP-8 belonged to ST42, while Co-CRKP-10 and Co-CRKP-15 were ST16 and ST231, respectively. These high-risk clones carrying multidrug resistance and virulence genes, implicated in numerous outbreaks, have spread worldwide. Our findings emphasize the necessity for effective treatment of hospital wastes to restrict the spread of clinical isolates into aquatic environments.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A One Health exploration of antimicrobial resistance in Escherichia coli originated from urban and rural lakes ecosystem. 源自城市和农村湖泊生态系统的大肠杆菌抗菌药耐药性的综合健康探索。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae095

Priyanka Priyanka, Prem Raj Meena, Dharma Raj, Purnima Mishra, Anand Kumar Jha, K Siddaardha Duggirala, Akshay Dhanokar, Amit Kumar, Anuj Rana, Arvind Pratap Singh

{"title":"A One Health exploration of antimicrobial resistance in Escherichia coli originated from urban and rural lakes ecosystem.","authors":"Priyanka Priyanka, Prem Raj Meena, Dharma Raj, Purnima Mishra, Anand Kumar Jha, K Siddaardha Duggirala, Akshay Dhanokar, Amit Kumar, Anuj Rana, Arvind Pratap Singh","doi":"10.1093/lambio/ovae095","DOIUrl":"10.1093/lambio/ovae095","url":null,"abstract":"Antimicrobial resistance (AMR) has become one of the most serious threats to One Health. Aquatic environments are an ideal non-clinical AMR reservoir and can act as a key battlefront for tackling the AMR. However, AMR data using the One Health approach remain scarce in aquatic environments worldwide. Here, we extensively assessed AMR in Escherichia coli isolated from urban and rural lake ecosystems using the One Health perspective. A total of 162 E. coli isolates obtained from lakes were tested against 25 antimicrobials using an in-vitro antimicrobial susceptibility testing method. A low (2%) to moderate (45%) drug resistance rate was found for all antimicrobials used in human/veterinary medicine or animal/plant agriculture. However, <80% E. coli isolates exhibited multidrug resistance (MDR) phenotype to highly important (amikacin, gentamicin, trimethoprim) or critically important (amoxicillin, ampicillin, colistin) drugs of both human and veterinary medicine. Of concern, >50% of E. coli isolates exhibited MDR to drugs used as last-resorts (chloramphenicol, colistin) or as frontline (nitrofurantoin, sulfamethoxazole, ampicillin, gentamicin) against E. coli infections. In conclusion, the presence of MDR E. coli strains in urban or rural lake ecosystems highlights their possible role as AMR reservoirs with potential One Health risks.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0