Letters in Applied Microbiology最新文献_第10页

Antibacterial mechanism of the methanol extract of Thamnolia subuliformis (Ehrh.) W. Culb against Staphylococcus aureus. Thamnolia subuliformis (Ehrh.) W. Culb 甲醇提取物对金黄色葡萄球菌的抗菌机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/lambio/ovae073

Menglong Liu, Hongqiao Tian, Jiana Zhu, Haiyan Ding

{"title":"Antibacterial mechanism of the methanol extract of Thamnolia subuliformis (Ehrh.) W. Culb against Staphylococcus aureus.","authors":"Menglong Liu, Hongqiao Tian, Jiana Zhu, Haiyan Ding","doi":"10.1093/lambio/ovae073","DOIUrl":"10.1093/lambio/ovae073","url":null,"abstract":"Thamnolia subuliformis (Ehrh.) W. Culb is a species of lichen with edible and medicinal applications in China. Our previous studies demonstrated that the methanol extract of Thamnolia subuliformis (METS) exhibits broad antibacterial activity and stability against foodborne pathogens. This study aimed to investigate the antibacterial mechanism of METS against Staphylococcus aureus using nontargeted metabolomics, focusing on cell wall and membrane damage. The results revealed that the minimum inhibitory concentration (MIC) was 0.625 mg ml-1 and that METS had good biosafety at this concentration. METS caused significant damage to the cell wall and membrane integrity, based on both morphological observation by electron microscopy and the leakage of alkaline phosphatase, protein, and nucleic acid in the cell cultures. Treatment with METS at the MIC disrupted the lipid metabolism of S. aureus, causing a decrease in the metabolism of various phospholipids and sphingolipids in the cell membrane and an increase in the ratio of saturated fatty acids to unsaturated fatty acids. Moreover, it influenced intracellular amino acid and energy metabolism. These results shed light on the antibacterial mechanism of METS against S. aureus while also serving as a reference for the further development of natural antibacterial compounds derived from Thamnolia subuliformis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria. 评估密切相关的氨氧化细菌的氧动力学参数。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/lambio/ovae076

Pallabita Saha, Ann-Kathrin Kniggendorf, Andreas Pommerening-Röser, Regina Nogueira

{"title":"Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria.","authors":"Pallabita Saha, Ann-Kathrin Kniggendorf, Andreas Pommerening-Röser, Regina Nogueira","doi":"10.1093/lambio/ovae076","DOIUrl":"10.1093/lambio/ovae076","url":null,"abstract":"The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis-Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l-1, 0.47 ± 0.09 mg l-1, and 0.28 ± 0.08 mg l-1, and a Vmax[O2] of 0.07 ± 0.04 pg h-1cell-1, 0.25 ± 0.06 pg h-1cell-1, and 0.02 ± 0.001 pg h-1cell-1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of antimicrobial multicyclic terpenoids from the medicinal plant Salvia officinalis and development of a formulation against clinical Staphylococcus aureus strains. 从药用植物丹参中分离和鉴定抗菌多环萜类化合物，并开发针对临床金黄色葡萄球菌菌株的制剂。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/lambio/ovae077

Gislaine Aparecida Purgato, Mayra Soares Píccolo, Maria Aparecida Scatamburlo Moreira, Virgínia Ramos Pizziolo, Gaspar Diaz-Muñoz, Ciro César Rossi, Marisa Alves Nogueira Diaz

{"title":"Isolation and identification of antimicrobial multicyclic terpenoids from the medicinal plant Salvia officinalis and development of a formulation against clinical Staphylococcus aureus strains.","authors":"Gislaine Aparecida Purgato, Mayra Soares Píccolo, Maria Aparecida Scatamburlo Moreira, Virgínia Ramos Pizziolo, Gaspar Diaz-Muñoz, Ciro César Rossi, Marisa Alves Nogueira Diaz","doi":"10.1093/lambio/ovae077","DOIUrl":"10.1093/lambio/ovae077","url":null,"abstract":"Staphylococcus aureus, particularly multi-drug resistant strains, presents significant challenges in dairy farming due to its role in causing bovine mastitis, which leads to substantial economic losses and limited treatment options. Seeking alternative therapies, we investigated the potential of a topical formulation derived from the medicinal herb Salvia officinalis to combat S. aureus growth and biofilms associated with bovine mastitis. Through systematic extraction in different solvents and fractionation by column chromatography, we isolated and identified three key multicyclic terpenoids-ferruginol, sugiol, and sclareol-exhibiting significant antimicrobial activity. The formulation effectively inhibited biofilm formation, with minimum inhibitory concentration (MIC) values ranging from 0.09 to 0.74 mg ml-1 against clinical S. aureus strains, comparable to or lower than those of the pure compounds. Moreover, it displayed robust anti-adhesive properties, reducing biofilm formation by 20%-79% at subinhibitory concentrations. Furthermore, the formulation successfully disrupted pre-existing biofilms, achieving reductions ranging from 30% to 82%. Cytotoxicity assays confirmed the safety of the formulation on mammary epithelial cells, with cell viability maintained at 100% at MIC. Our findings underscore the therapeutic potential of Sa. officinalis-derived compounds in managing bovine mastitis caused by S. aureus, emphasizing their antimicrobial efficacy and safety profile.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of plant growth-promoting bacteria isolated from arid heavy metal contaminated environments in alleviating salt and water stresses in alfalfa. 从干旱重金属污染环境中分离出的植物生长促进菌在缓解紫花苜蓿盐和水胁迫方面的潜力。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/lambio/ovae075

Anas Raklami, Aiman Slimani, Khalid Oufdou, Martin Jemo, Noura Bechtaoui, Boujamaa Imziln, Abdelilah Meddich, Salvadora Navarro-Torre, Ignacio D Rodríguez-Llorente, Eloísa Pajuelo

{"title":"The potential of plant growth-promoting bacteria isolated from arid heavy metal contaminated environments in alleviating salt and water stresses in alfalfa.","authors":"Anas Raklami, Aiman Slimani, Khalid Oufdou, Martin Jemo, Noura Bechtaoui, Boujamaa Imziln, Abdelilah Meddich, Salvadora Navarro-Torre, Ignacio D Rodríguez-Llorente, Eloísa Pajuelo","doi":"10.1093/lambio/ovae075","DOIUrl":"10.1093/lambio/ovae075","url":null,"abstract":"Co-evolution of plant beneficial microbes in contaminated environments enhances plant growth and mitigates abiotic stress. However, few studies on heavy metal (HM) tolerant plant growth-promoting bacteria (PGPB) promoting crop growth in Morocco's farming areas affected by drought and salinity are available. Plant associated bacteria tolerant to HM and able to produce indole acetic acid and siderophores, display ACC-deaminase activity and solubilize phosphate, were isolated from long-term metal exposed environments. Tolerance to HM and biofilms formation in the absence or presence of HM were assessed. A consortium including two Ensifer meliloti strains (RhOL6 and RhOL8), one Pseudomonas sp. strain (DSP17), and one Proteus sp. strain (DSP1), was used to inoculate alfalfa (Medicago sativa) seedlings under various conditions, namely, salt stress (85 mM) and water stress (30% water holding capacity). Shoot and root dry weights of alfalfa were measured 60 days after sowing. In the presence of HM, DSP17 showed the greatest auxin production, whereas RhOL8 had the highest ACC-deaminase activity and DSP17 formed the densest biofilm. Root dry weight increased 138% and 195% in salt and water stressed plants, respectively, regarding non-inoculated controls. Our results confirm the improvement of alfalfa growth and mitigation of salt and drought stress upon inoculation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Competitive exclusion products as an antimicrobial alternative to control Salmonella Heidelberg in broilers. 竞争性排除产品作为控制肉鸡海德堡沙门氏菌的抗菌剂替代品。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/lambio/ovae071

Isis M M Kolososki, Mauro M S Saraiva, Camila F Nascimento, Isabella C Campos, Túlio S Lima, Viviane A Ferreira, André L C Rabelo, Adriana M Almeida, Angelo Berchieri Junior

{"title":"Competitive exclusion products as an antimicrobial alternative to control Salmonella Heidelberg in broilers.","authors":"Isis M M Kolososki, Mauro M S Saraiva, Camila F Nascimento, Isabella C Campos, Túlio S Lima, Viviane A Ferreira, André L C Rabelo, Adriana M Almeida, Angelo Berchieri Junior","doi":"10.1093/lambio/ovae071","DOIUrl":"10.1093/lambio/ovae071","url":null,"abstract":"Intestinal infections caused by non-typhoidal Salmonella spp., along with antimicrobial resistance spread are a major food safety concern worldwide. Here, we evaluate the potential of competitive exclusion products developed by anaerobic or aerobic conditions to control systemic infection, cecal colonization, fecal excretion, and improve the intestinal health in broilers challenged by Salmonella Heidelberg (SH). A total of 105 day-old chickens were randomly distributed into three experimental groups: A (untreated control), B (treated with anaerobic culture), and C (treated with aerobic culture). During 21 days, morphometric parameters of the small intestine were analyzed using microscopy, fecal excretions by cloacal swabs, systemic infection, and cecal colonization by colony-forming unit counts (CFU/g). The results indicated the lowest number of positive swabs (45.33%) recovered from Group C, followed by Group B (71.8%) and Group A (85.33%). The bacterial enumeration revealed the lowest amounts in Group C at the necropsy realized in 5-, 7-, and 14-days post-infection (DPI) (P = 0.0010, P = 0.0048, and P = 0.0094, respectively). Statistical differences between intestinal morphometrics were observed in the Group C at 21 DPI. Our results suggest that the product developed under aerobic conditions can improve intestinal health, protecting birds against SH.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofertilizer and biostimulant potentials of phosphate-solubilizing Bacillus subtilis subsp. subtilis M1 strain and silicon in improving low phosphorus availability tolerance in rosemary. 磷溶解芽孢杆菌亚种 M1 菌株和硅在提高迷迭香耐低磷能力方面的生物肥料和生物刺激潜力

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-08-05 DOI: 10.1093/lambio/ovae072

Hamid Msaad, Nadia Lamsaadi, Omar Farssi, Aziz Oubenali, Soukaina Lahmaoui, Abdelali Boulli, Cherki Ghoulam, Ahmed El Moukhtari, Mohamed Farissi

{"title":"Biofertilizer and biostimulant potentials of phosphate-solubilizing Bacillus subtilis subsp. subtilis M1 strain and silicon in improving low phosphorus availability tolerance in rosemary.","authors":"Hamid Msaad, Nadia Lamsaadi, Omar Farssi, Aziz Oubenali, Soukaina Lahmaoui, Abdelali Boulli, Cherki Ghoulam, Ahmed El Moukhtari, Mohamed Farissi","doi":"10.1093/lambio/ovae072","DOIUrl":"10.1093/lambio/ovae072","url":null,"abstract":"The present study aimed to evaluate the single and combined effects of Si exogenous treatment and Bacillus subtilis subsp. subtilis M1 strain inoculation on rosemary tolerance to low phosphorus (P) availability. Hence, rosemary plants were fertilized with 250 µmol Ca3HPO4 (stressed plants) or 250 µmol KH2PO4 (control plants) under Si treatment and B. subtilis M1 strain inoculation. P starvation negatively affected rosemary growth and its P nutrition. However, exogenous Si supply or B. subtilis M1 strain inoculation significantly (P < 0.001) alleviated the deficiency-induced effects and significantly improved rhizogenesis, acid phosphatase activity, P uptake, and eventually dry weight of shoot and root. Moreover, Si-treatment and/or B. subtilis M1 strain inoculation significantly (P < 0.001) reduced the oxidative damage, in terms of malondialdehyde and hydrogen peroxide accumulation. This was found positively correlated with the higher superoxide dismutase activity, and the elevated non-enzymatic antioxidant molecules accumulation, including total polyphenols in Si-treated and inoculated P-deficient plants. Taken together, Si supplementation and/or B. subtilis M1 strain inoculation could be a good strategy to sustain rosemary plant growth under P starvation conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of novel species-specific and genus-specific primers for the detection of Babaco Mosaic Virus (BabMV). 开发用于检测巴布科马赛克病毒（BabMV）的新型物种特异性和属特异性引物。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-07-01 DOI: 10.1093/lambio/ovae070

Martina Albuja-Quintana, Carolina E Armijos, Andrea Montero-Oleas, Maria de Lourdes Torres

{"title":"Development of novel species-specific and genus-specific primers for the detection of Babaco Mosaic Virus (BabMV).","authors":"Martina Albuja-Quintana, Carolina E Armijos, Andrea Montero-Oleas, Maria de Lourdes Torres","doi":"10.1093/lambio/ovae070","DOIUrl":"10.1093/lambio/ovae070","url":null,"abstract":"Babaco is a hybrid cultivar native to the Andean region of Ecuador and Colombia, commercially attractive for its fruit. Babaco production in Ecuador faces losses from plant pathogens like babaco mosaic virus (BabMV), an RNA virus that causes chlorosis, leaf mottling, and deformation. Phylogenetic studies link BabMV to papaya mosaic virus (PapMV), alternanthera mosaic virus, and senna mosaic virus. To address this threat, we developed novel species-specific primers to detect BabMV targeting a 165 bp region of the coat protein (CP). Genus-specific primers were designed to validate the species-specific primers and attest their ability to discriminate between BabMV and its closest relatives. These primers targeted a 175 bp fragment of the CP region. The most effective sets of primers were chosen for reverse transcription polymerase chain reaction (RT-PCR) and SYBR® Green-based quantitative reverse transcription polymerase chain reaction (RT-qPCR) in symptomatic and asymptomatic babaco plants. Among 28 plants tested, 25 were positive and 3 were negative for BabMV using species-specific and genus-specific primers in RT-PCR and RT-qPCR, while the PapMV positive control was detected with the genus-specific primers and was negative for the species-specific primers. These primers represent a valuable molecular tool for detecting BabMV, potentially enhancing crop management.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prunus persica leaves aqueous extract mediated biosynthesis of Ag nanoparticles and assessment of its anti-quorum sensing potential against Hafnia species. 柿树叶水提取物介导的银纳米颗粒生物合成及其对 Hafnia sp.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-07-01 DOI: 10.1093/lambio/ovae055

Xiqian Tan, Jianbo Pei, Defu Zhang, Fangchao Cui, Dangfeng Wang, Xuepeng Li, Jianrong Li

{"title":"Prunus persica leaves aqueous extract mediated biosynthesis of Ag nanoparticles and assessment of its anti-quorum sensing potential against Hafnia species.","authors":"Xiqian Tan, Jianbo Pei, Defu Zhang, Fangchao Cui, Dangfeng Wang, Xuepeng Li, Jianrong Li","doi":"10.1093/lambio/ovae055","DOIUrl":"10.1093/lambio/ovae055","url":null,"abstract":"Hafnia sp. was one of the specific spoilage bacteria in aquatic products, and the aim of the study was to investigate the inhibition ability of the silver nanoparticles (AgNPs) biosynthesis by an aqueous extract of Prunus persica leaves toward the spoilage-related virulence factors of Hafnia sp. The synthesized P-AgNPs were spherical, with a mean particle size of 36.3 nm and zeta potential of 21.8 ± 1.33 mV. In addition, the inhibition effects of P-AgNPs on the growth of two Hafnia sp. strains and their quorum sensing regulated virulence factors, such as the formation of biofilm, secretion of N-acetyl-homoserine lactone (AHLs), proteases, and exopolysaccharides, as well as their swarming and swimming motilities were evaluated. P-AgNPs had a minimum inhibitory concentration (MIC) of 64 μg ml-1 against the two Hafnia sp. strains. When the concentration of P-AgNPs was below MIC, it could inhibit the formation of biofilms by Hafnia sp at 8-32 μg ml-1, but it promoted the formation of biofilms by Hafnia sp at 0.5-4 μg ml-1. P-AgNPs exhibited diverse inhibiting effects on AHLs and protease production, swimming, and swarming motilities at various concentrations.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of two fillers and process conditions on the water treatment efficiency of a continuous packed bed biofilm reactor. 两种填料和工艺条件对连续填料床生物膜反应器水处理效率的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-07-01 DOI: 10.1093/lambio/ovae060

Yijing Fang, Zhifei Li, Guangjun Wang, Yun Xia, Kai Zhang, Wangbao Gong, Ermeng Yu, Wenping Xie, Hongyan Li, Jingjing Tian, Jun Xie, Qiyou Xu

{"title":"Effects of two fillers and process conditions on the water treatment efficiency of a continuous packed bed biofilm reactor.","authors":"Yijing Fang, Zhifei Li, Guangjun Wang, Yun Xia, Kai Zhang, Wangbao Gong, Ermeng Yu, Wenping Xie, Hongyan Li, Jingjing Tian, Jun Xie, Qiyou Xu","doi":"10.1093/lambio/ovae060","DOIUrl":"10.1093/lambio/ovae060","url":null,"abstract":"This study evaluated the treatment efficiency of two selected fillers and their combination for improving the water quality of aquaculture wastewater using a packed bed biofilm reactor (PBBR) under various process conditions. The fillers used were nanosheet (NS), activated carbon (AC), and a combination of both. The results indicated that the use of combined fillers and the hydraulic retention time (HRT) of 4 h significantly enhanced water quality in the PBBR. The removal rates of chemical oxygen demand, NO2-─N, total suspended solids（TSS）, and chlorophyll a were 63.55%, 74.25%, 62.75%, and 92.85%, respectively. The microbiota analysis revealed that the presence of NS increased the abundance of microbial phyla associated with nitrogen removal, such as Nitrospirae and Proteobacteria. The difference between the M1 and M2 communities was minimal. Additionally, the microbiota in different PBBR samples displayed similar preferences for carbon sources, and carbohydrates and amino acids were the most commonly utilized carbon sources by microbiota. These results indicated that the combination of NS and AC fillers in a PBBR effectively enhanced the treatment efficiency of aquaculture wastewater when operated at an HRT of 4 h. The findings provide valuable insights into optimizing the design of aquaculture wastewater treatment systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of an LysR family transcriptional regulator that activates motility and flagellar gene expression in Vibrio parahaemolyticus. 鉴定可激活副溶血性弧菌运动和鞭毛基因表达的 LysR 家族转录调节器。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-07-01 DOI: 10.1093/lambio/ovae059

Jingyang Chang, Yining Zhou, Xue Li, Miaomiao Zhang, Yiquan Zhang, Bin Ni, Renfei Lu

{"title":"Identification of an LysR family transcriptional regulator that activates motility and flagellar gene expression in Vibrio parahaemolyticus.","authors":"Jingyang Chang, Yining Zhou, Xue Li, Miaomiao Zhang, Yiquan Zhang, Bin Ni, Renfei Lu","doi":"10.1093/lambio/ovae059","DOIUrl":"10.1093/lambio/ovae059","url":null,"abstract":"Vibrio parahaemolyticus utilizes a polar flagellum for swimming in liquids and employs multiple lateral flagella to swarm on surfaces and in viscous environments. The VPA0961 protein is an LysR family transcriptional regulator that can regulate the swimming and swarming motility of V. parahaemolyticus, but the detailed regulatory mechanisms are not yet fully understood. Herein, we designated the protein as AcsS, which stands for activator of swimming and swarming motility. Our data provided evidence that deleting the acsS gene significantly reduced both swimming and swarming motility of V. parahaemolyticus. Furthermore, AcsS was found to activate the expression of both polar (flgA, flgM, flgB, and flgK) and lateral (motY, fliM, lafA, and fliD) flagellar genes. Overexpression of AcsS in Escherichia coli induced the expression of flgA, motY, and lafA, but did not affect the expression of flgB, flgK, flgM, fliM, and fliD. Interestingly, His-tagged AcsS did not bind to the upstream DNA regions of all the tested genes, suggesting indirect regulation. In conclusion, AcsS positively regulated the swimming and swarming motility of V. parahaemolyticus by activating the transcription of polar and lateral flagellar genes. This work enriched our understanding of the gene expression regulation within the dual flagellar systems of V. parahaemolyticus.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0