Letters in Applied Microbiology最新文献_第9页

A dye elution method for the quantification of insecticidal crystal proteins from Bacillus thuringiensis and its compatibility with the presence of agro-industrial raw materials and waste products. 定量测定苏云金芽孢杆菌杀虫晶体蛋白的染料洗脱法及其与农用工业原料和废品的兼容性。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae024

María Isabel Mentel, Flavia Del Valle Loto, Mario Domingo Baigori, Licia María Pera

{"title":"A dye elution method for the quantification of insecticidal crystal proteins from Bacillus thuringiensis and its compatibility with the presence of agro-industrial raw materials and waste products.","authors":"María Isabel Mentel, Flavia Del Valle Loto, Mario Domingo Baigori, Licia María Pera","doi":"10.1093/lambio/ovae024","DOIUrl":"10.1093/lambio/ovae024","url":null,"abstract":"The insecticidal crystal proteins produced by Bacillus thuringiensis during sporulation are active ingredients against lepidopteran, dipteran, and coleopteran insects. Several methods have been reported for their quantification, such as crystal counting, ELISA, and SDS-PAGE/densitometry. One of the major tasks in industrial processes is the analysis of raw material dependency and costs. Thus, the crystal protein quantification method is expected to be compatible with the presence of complex and inexpensive culture medium components. This work presents a revalidated elution-based method for the quantification of insecticidal crystal proteins produced by the native strain B. thuringiensis RT. To quantify proteins, a calibration curve was generated by varying the amount of BSA loaded into SDS-PAGE gels. First, SDS-PAGE was performed for quality control of the bioinsecticide. Then, the stained protein band was excised from 10% polyacrylamide gel and the protein-associated dye was eluted with an alcoholic solution of SDS (3% SDS in 50% isopropanol) during 45 min at 95°C. This protocol was a sensitive procedure to quantify proteins in the range of 2.0-10.0 µg. As proof of concept, proteins of samples obtained from a complex fermented broth were separated by SDS-PAGE. Then, Cry1 and Cry2 proteins were properly quantified.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol strategies against Botrytis cinerea in viticulture: evaluating the efficacy and mode of action of selected winemaking yeast strains. 葡萄栽培中灰霉病的生物防治策略：评估选定酿酒酵母菌株的功效和作用模式。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae026

Artemis Tsioka, Konstantina Psilioti Dourmousi, Eirini G Poulaki, Georgios Papoutsis, Sotirios E Tjamos, Danai Gkizi

{"title":"Biocontrol strategies against Botrytis cinerea in viticulture: evaluating the efficacy and mode of action of selected winemaking yeast strains.","authors":"Artemis Tsioka, Konstantina Psilioti Dourmousi, Eirini G Poulaki, Georgios Papoutsis, Sotirios E Tjamos, Danai Gkizi","doi":"10.1093/lambio/ovae026","DOIUrl":"10.1093/lambio/ovae026","url":null,"abstract":"Botrytis cinerea poses a recurring threat to viticulture, causing significant yield losses each year. The study explored the biocontrol capabilities of commercially used winemaking yeasts as a strategy to manage B. cinerea in grape berries. The winemaking yeast strains-Saccharomyces cerevisiae ES181, Saccharomyces pastorianus KBG6, S. cerevisiae BCS103, Lachancea thermotolerans Omega, and Torulaspora delbrueckii TD291-reduced B. cinerea growth and conidiation in vitro. Furthermore, they demonstrated a decreased disease severity and number of conidia in grape berries. Among these strains, S. cerevisiae BCS103 was the most effective, inducing the expression of the defense-related gene PR4 in berries. Its diffusible compounds and volatile organic compounds also reduced the expression of BcLTF2, a positive regulator of B. cinerea conidiogenesis. The examined winemaking yeast strains, especially S. cerevisiae BCS103, demonstrated effective inhibition of B. cinerea in vitro and in grape berries, influencing key defense genes and reducing BcLTF2 expression, offering potential solutions for disease management in viticulture. The study underscores the promise of commercially available winemaking yeast strains as eco-friendly tools against B. cinerea in viticulture. Leveraging their safety and existing use in winemaking offers a potential avenue for sustainable disease management.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three novel multiplex PCR assays for rapid detection of virulence, antimicrobial resistance, and toxin genes in Acinetobacter calcoaceticus-baumannii complex species. 用于快速检测醋酸钙化杆菌-鲍曼尼氏菌复合菌毒力、抗菌药耐药性和毒素基因的三种新型多重 PCR 检测方法。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae027

Alexander A Sheikh, Danielle Schneiderman, Ellen M E Sykes, Ayush Kumar, Wen Chen, David R Lapen, Izhar U H Khan

{"title":"Three novel multiplex PCR assays for rapid detection of virulence, antimicrobial resistance, and toxin genes in Acinetobacter calcoaceticus-baumannii complex species.","authors":"Alexander A Sheikh, Danielle Schneiderman, Ellen M E Sykes, Ayush Kumar, Wen Chen, David R Lapen, Izhar U H Khan","doi":"10.1093/lambio/ovae027","DOIUrl":"10.1093/lambio/ovae027","url":null,"abstract":"The Acinetobacter calcoaceticus-baumannii (ACB) complex is an often-overlooked group of nosocomial pathogens with a significant environmental presence. Rapid molecular screening methods for virulence, antimicrobial resistance, and toxin (VAT) genes are required to investigate the potential pathogenicity of environmental isolates. This study aimed to develop and apply novel ACB complex-specific multiplex PCR (mPCR) primers and protocols for the rapid detection of eight VAT genes. We optimized three single-tube mPCR assays using reference DNA from ACB complex and other Acinetobacter species. These assays were then applied to detect VAT genes in cultured ACB complex isolates recovered from clinical and environmental sources. Widespread detection of VAT genes in environmental isolates confirmed the validity, functionality, and applicability of these novel assays. Overall, the three newly developed ACB complex species-specific mPCR assays are rapid and simple tools that can be adopted in diagnostic and clinical lab settings. The detection of VAT genes in environmental isolates suggests that environmental niches could serve as a reservoir for potentially pathogenic ACB complex and warrants further investigation. The newly developed mPCR assays are specific, sensitive, and efficient, making them well-suited for high-throughput screening in epidemiological studies and evaluating the potential pathogenicity of ACB complex recovered from various sources.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in milk, udder skin, and fecal microbiota and their relationships with blood metabolites and milk composition in dairy cows. 奶牛牛奶、乳房皮肤和粪便微生物群的变化及其与血液代谢物和牛奶成分的关系。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae014

AoDaohu, Akada Karin, Gathinji Peter Kiiru, Nishino Naoki

{"title":"Variations in milk, udder skin, and fecal microbiota and their relationships with blood metabolites and milk composition in dairy cows.","authors":"AoDaohu, Akada Karin, Gathinji Peter Kiiru, Nishino Naoki","doi":"10.1093/lambio/ovae014","DOIUrl":"10.1093/lambio/ovae014","url":null,"abstract":"This study examined the milk, udder skin, feces, and bedding microbiota in a dairy farm. Blood metabolites concentration and milk composition were also determined to examine their relationship with variations in the microbiota. Samples were collected from 10 healthy cows during the summers of 2018 and 2020. Milk protein, fat, and solid-not-fat contents were higher, and blood urea nitrogen and nonesterified fatty acid levels were lower in the 2020 samples. Principal coordinate analysis demonstrated that milk, udder skin, and fecal microbiota were separate groups. Year-to-year differences were distinct for milk and udder skin microbiota; however, the fecal microbiota of the 2018 and 2020 samples were similar. The bedding microbiota grouped with the udder skin microbiota of the 2018 samples. Although nonpathogens found as prevalent taxa in udder skin microbiota were likely to be found as abundant taxa in milk microbiota, selection and elimination occurred during transmission. Network analysis suggested that bacterial taxa of milk, udder skin, and fecal microbiota were unrelated to blood metabolites and milk composition, regardless of pathogens or nonpathogens.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular polymeric substances in electroactive biofilms play a crucial role in improving the efficiency of microbial fuel and electrolysis cells. 电活性生物膜中的胞外聚合物物质在提高微生物燃料电池和电解电池的效率方面发挥着至关重要的作用。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae017

Tunc Catal, Hong Liu, Burak Kilinc, Kaan Yilancioglu

{"title":"Extracellular polymeric substances in electroactive biofilms play a crucial role in improving the efficiency of microbial fuel and electrolysis cells.","authors":"Tunc Catal, Hong Liu, Burak Kilinc, Kaan Yilancioglu","doi":"10.1093/lambio/ovae017","DOIUrl":"10.1093/lambio/ovae017","url":null,"abstract":"In microbial electrochemical cells (MECs), electroactive microbial biofilms can transmit electrons from organic molecules to anodes. To further understand the production of anodic biofilms, it is essential to investigate the composition and distribution of extracellular polymeric substance (EPS) in the MECs. In this study, the structure of EPS was examined in microbial electrolysis cells from mixed cultures forming biofilm using carbon fiber fabric anode. EPS was extracted from the anode biofilm of microbial electrolysis cells inoculated with mixed microbial culture. The anode biofilm yielded 0.4 mg of EPS, of which 51.2% was humic substance, 16.2% was protein, 12.6% was carbohydrates, and 20% consisted of undetermined substances. Using epifluorescence microscopy, the composition of bacterial cells and their location inside EPS were studied, and the distribution of microbial communities was compared based on current density results in the presence of various carbohydrates. On the electrode surface, bacteria and EPS gathered or overlapped in various locations can affect microbial electrochemical performance. Our findings showed that EPS formation in electroactive biofilms would be important for enhanced efficiency of electricity- or hydrogen-producing microbial electrolysis cells.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139898113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Lactobacillus acidophilus strain and its anti-obesity effect in a diet induced obese murine model. 分离嗜酸乳杆菌菌株及其在饮食诱导肥胖小鼠模型中的抗肥胖作用

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae021

Yanyi Zheng, Silu Zhang, Zhizhu Zhang, Tengxun Zhang, Xin Teng, Guoxun Xiao, Song Huang

引用次数: 0

Validation of rapid detection methods for Salmonella enterica in green chile. 验证绿色智利中肠炎沙门氏菌的快速检测方法。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae011

Raghda Kailany, Yatziri Presmont, Ruben Zapata, James Owusu-Kwarteng, Willis Fedio

{"title":"Validation of rapid detection methods for Salmonella enterica in green chile.","authors":"Raghda Kailany, Yatziri Presmont, Ruben Zapata, James Owusu-Kwarteng, Willis Fedio","doi":"10.1093/lambio/ovae011","DOIUrl":"10.1093/lambio/ovae011","url":null,"abstract":"The objective of this study is to validate the US Food and Drug Administration (FDA) rea-time polymerase chain reaction (qPCR) assay, the Neogen Amplified Nucleic Single Temperature Reaction (ANSR) assay, and the Vitek ImmunoDiagnostic Assay System (VIDAS) SLM procedure against the FDA cultural procedure for Salmonella detection in green chile pepper. Green chile was artificially contaminated with Salmonella according to the FDA guidelines (FDA. Guidelines for the Validation of Microbiological Methods for the FDA Foods Program, 3rd Edition. 2019. www.fda.gov/media/83812/download?attachment (17 March 2024, date last accessed)) at a fractional recovery level (where 50%-25% tests positive and at a level +1 log greater for each organism tested). Enriched samples were tested directly by the ANSR Salmonella test and by qPCR, and were subcultured into Rappaport-Vassiliadis and tetrathionate brilliant green broth for cultural detection and qPCR. For the VIDAS-SLM assay, the selective enrichments were further cultured in M broth before testing. Presumptive salmonellae were confirmed with biochemical tests, serology, and qPCR. All three rapid assays were compared favorably with the FDA-BAM (Bacteriological Analytical Manual) method. No significant differences at P < .05 were found between the procedures using McNemar's χ2 test. The three procedures were found to be rapid and reliable alternatives to cultural detection of Salmonella enterica in green chile.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139746845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Opportunistic pathogens increased and probiotics or short-chain fatty acid-producing bacteria decreased in the intestinal microbiota of pneumonia inpatients during SARS-CoV-2 Omicron variant epidemic. 在 SARS-CoV-2 Omicron 变体流行期间，肺炎住院患者肠道微生物群中的机会性病原体增加，益生菌或产短链脂肪酸的细菌减少。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae022

Fan Huang, Min Luo, Jun Peng, Shide Liu, Jinlei He

{"title":"Opportunistic pathogens increased and probiotics or short-chain fatty acid-producing bacteria decreased in the intestinal microbiota of pneumonia inpatients during SARS-CoV-2 Omicron variant epidemic.","authors":"Fan Huang, Min Luo, Jun Peng, Shide Liu, Jinlei He","doi":"10.1093/lambio/ovae022","DOIUrl":"10.1093/lambio/ovae022","url":null,"abstract":"The global pandemic of COVID-19 has been over four years, and the role of intestinal microbiota in the occurrence and development of COVID-19 needs to be further clarified. During the outbreak of SARS-CoV-2 Omicron variant in China, we analyzed the intestinal microbiome in fecal samples from inpatients with pneumonia and normal individuals in January 2023. The microbiota composition, alpha diversity, beta diversity, differential microbial community, co-occurrence networks, and functional abundance were analyzed. The results showed significant differences in microbiota composition between the two groups. In pneumonia group, the abundance of Bifidobacterium, Blautia, Clostridium, and Coprococcus decreased, while the abundance of Enterococcus, Lactobacillus, and Megamonas increased. Through LEfSe analysis, 37 marker microbiota were identified in pneumonia group. Co-occurrence network analysis found that Lachnospiraceae was critical for the interaction of intestinal microbiota, and the anti-inflammatory bacteria Blautia was negatively correlated with the pro-inflammatory bacteria Ruminococcus. Functional prediction found the up-regulation of steroid biosynthesis, geraniol degradation, and mRNA surveillance pathway in pneumonia group. In conclusion, opportunistic pathogens increased and probiotics, or short-chain fatty acid-producing bacteria, decreased in the intestinal microbiota of pneumonia inpatients during the Omicron epidemic. Blautia could be used as a probiotic in the treatment of pneumonia patients in the future.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant and L-asparaginase activities of culturable endophytic fungi from ornamental Dendrobium orchids. 观赏石斛兰中可培养的内生真菌的抗氧化和 L-天冬酰胺酶活性。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovad096

Ru Wei Chua, Keang Peng Song, Adeline Su Yien Ting

{"title":"Antioxidant and L-asparaginase activities of culturable endophytic fungi from ornamental Dendrobium orchids.","authors":"Ru Wei Chua, Keang Peng Song, Adeline Su Yien Ting","doi":"10.1093/lambio/ovad096","DOIUrl":"10.1093/lambio/ovad096","url":null,"abstract":"This study reports the antioxidant potential and L-asparaginase production of culturable fungal endophytes from Dendrobium orchids in Malaysia. Twenty-nine isolates were screened using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine their free radical scavenging activities and antioxidant capacity (IC50 and AEAC). L-asparaginase production of fungal endophytes was detected by the qualitative plate assay, and the enzyme activities estimated via the Nesslerization method. All 29 endophytic isolates exhibited various degrees of radical scavenging activities (35.37%-77.23%), with Fusarium fujikuroi (D1) identified as having the highest antioxidant capacity (IC50 6.097 mg/mL) and the highest AEAC value (11.55 mg/g). For L-asparaginase production, the majority of the isolates (89.66%) showed positive results, especially among the culturable species of Fusarium, Trichoderma, and Daldinia. Most Fusarium spp. were able to produce L-asparaginase (80.77%), but the highest L-asparaginase activity was detected in Daldinia eschscholtzii (D14) with 2.128 units/mL. Results from this study highlighted the potential of endophytic fungi from medicinal orchids (Dendrobium sp.) as natural sources of bioactive compounds to be developed into novel antioxidants and anticancer drugs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10028119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ochratoxin A detoxification potentials of basil, chan, and chia seeds. 罗勒、陈皮和奇亚籽的赭曲霉毒素解毒潜能。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-03-01 DOI: 10.1093/lambio/ovae018

Hsin-Shun Tseng, Bing-Yi Lin, Yu-Fen Wang, Ya-Fan Liao

{"title":"Ochratoxin A detoxification potentials of basil, chan, and chia seeds.","authors":"Hsin-Shun Tseng, Bing-Yi Lin, Yu-Fen Wang, Ya-Fan Liao","doi":"10.1093/lambio/ovae018","DOIUrl":"10.1093/lambio/ovae018","url":null,"abstract":"The most toxic of the ochratoxins is ochratoxin A (OTA), which is primarily produced by species of Aspergillus and Penicillium that can be found in maize, wheat, coffee, red wine, and various grains. OTA induces immunotoxicity, nephrotoxicity, hepatotoxicity, teratogenicity, and carcinogenicity in both animals and humans. Thus, there is a need to identify mycotoxin detoxification agents that can effectively decontaminate OTA. Seeds of basil (Ocimum basilicum L.), chan (Hyptis suaveolens L.), and chia (Salvia hispanica L.) are functional foods capable of eliminating harmful substances. Despite this potential, the impact of these seeds on OTA detoxification remains unclear. This study reveals that milled basil, chan, and chia seeds adsorb significant levels of OTA, with chia demonstrating the highest adsorption capacity, followed by chan and basil seeds showing the least efficiency. Furthermore, milled basil, chan, and chia seeds effectively reduced OTA residues in artificial gastric and intestinal fluids, where they achieved up to 93% OTA adsorption in the former. In addition, these milled seeds were able to remove OTAs from canned, drip, and instant coffee. This study is the first to report the OTA elimination potential of basil, chan, and chia seeds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0