Letters in Applied Microbiology最新文献_第9页

Comparative evaluation of duplex and singleplex qPCR assays for detection of human-associated HF183 and general Bacteroides markers and implications for duplexing in digital PCR. 双工和单工qPCR检测人类相关HF183和一般拟杆菌标记的比较评价及其在数字PCR中双工的意义。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag011

Melody Lau, Paul Terence Monis, Alexandra Ruth Keegan, Barbara Drigo, Erica Donner, Brendon James King

{"title":"Comparative evaluation of duplex and singleplex qPCR assays for detection of human-associated HF183 and general Bacteroides markers and implications for duplexing in digital PCR.","authors":"Melody Lau, Paul Terence Monis, Alexandra Ruth Keegan, Barbara Drigo, Erica Donner, Brendon James King","doi":"10.1093/lambio/ovag011","DOIUrl":"10.1093/lambio/ovag011","url":null,"abstract":"Microbial source tracking (MST) using Bacteroidales markers provides information on faecal contamination in environmental waters. The marker BacUni targets a range of sources, while HF183 is primarily associated with human faeces. Duplex quantitative PCR (qPCR) assays allow simultaneous detection of both markers, though competition between primer-probe sets can affect accuracy. Digital PCR (dPCR) offers absolute quantification and higher sensitivity, but multiplexing can be challenging when marker concentrations differ. This study compared HF183 and BacUni quantification across singleplex and duplex qPCR formats and duplex dPCR. Synthetic standards revealed variability due to gBlock design and probe orientation, whereas environmental samples showed minimal format-dependent effects. Duplex qPCR provided comparable results to singleplex, with minor underestimation relative to dPCR. These small differences likely reflect the use of dPCR-quantified standards for qPCR calibration, rather than using DNA concentration-based calibration methods. Low-copy HF183 samples highlighted dPCR's superior sensitivity near detection limits. GoTaq qPCR was the most economical option, especially in duplex format, whereas dPCR offered competitive costs for duplexed samples with the advantage of absolute quantification. These findings demonstrate that duplex qPCR assays reliably quantify HF183 and BacUni in environmental waters, with dPCR serving as a robust complementary method for low-abundance or confirmatory analyses.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Cloning, expression and characterization of attachment-invasion locus protein (Ail) of Yersinia enterocolitica and its utilization in rapid detection by immunoassays. 修正：小肠结肠炎耶尔森菌附着-侵袭位点蛋白（Ail）的克隆、表达、鉴定及其在免疫快速检测中的应用。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag016

引用次数: 0

Synbiotic intervention with Bifidobacterium bifidum and prebiotics reduces enterohemorrhagic Escherichia coli colonization. 两歧双歧杆菌和益生元的合成干预可减少肠出血性大肠杆菌的定植。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag019

Wanqiu Sha, Anqi Su, Hong Cheng, Ning Dong, Jia Fu, Liang Zhu, Guoqing Tao, Yunxin Xue, Hongwei Cui, Dai Wang

{"title":"Synbiotic intervention with Bifidobacterium bifidum and prebiotics reduces enterohemorrhagic Escherichia coli colonization.","authors":"Wanqiu Sha, Anqi Su, Hong Cheng, Ning Dong, Jia Fu, Liang Zhu, Guoqing Tao, Yunxin Xue, Hongwei Cui, Dai Wang","doi":"10.1093/lambio/ovag019","DOIUrl":"10.1093/lambio/ovag019","url":null,"abstract":"This study evaluated the protective effect of combining Bifidobacterium bifidum with the prebiotics inulin and isomalto-oligosaccharides against enterohemorrhagic Escherichia coli infection and investigated the underlying mechanisms, using an integrated strategy of in vitro screening followed by in vivo validation. Among ten prebiotics screened, IMO and inulin were identified as optimal for promoting B. bifidum proliferation and acetate production (>2.5-fold), with IMO further enhancing bacterial adhesion to Caco-2 cells (four-fold). In a murine infection model, B. bifidum alone reduced total intestinal EHEC colonization, whereas inulin alone was ineffective. The combination of IMO and B. bifidum markedly suppressed EHEC colonization, particularly in the colon (five-fold reduction), while inulin provided no added benefit. Although fecal acetate levels were elevated in synbiotic groups, no linear correlation with EHEC clearance was observed, suggesting that protection may rely on mechanisms other than bulk acetate production-such as localized metabolite delivery or microbial ecological interactions. These findings demonstrate that an IMO-based synbiotic can effectively limit EHEC colonization through multifactorial modulation of the gut ecosystem. The presented strategy offers a rational framework for designing synbiotics to prevent foodborne infections.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered gut microbiota, SCFAs, and barrier integrity markers in Alzheimer's and Parkinson's disease patients. 阿尔茨海默病和帕金森病患者肠道微生物群、SCFAs和屏障完整性标志物的改变

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag010

Somayeh Ahmadi, Alka Hasani, Mohammad Yasdchi, Akbar Hasani, Vahdat Poortahmasbe, Farzaneh Rafie Sedaghat, Somaye Kakhki, Safa Najmi, Hammed Hamishehkar

{"title":"Altered gut microbiota, SCFAs, and barrier integrity markers in Alzheimer's and Parkinson's disease patients.","authors":"Somayeh Ahmadi, Alka Hasani, Mohammad Yasdchi, Akbar Hasani, Vahdat Poortahmasbe, Farzaneh Rafie Sedaghat, Somaye Kakhki, Safa Najmi, Hammed Hamishehkar","doi":"10.1093/lambio/ovag010","DOIUrl":"10.1093/lambio/ovag010","url":null,"abstract":"Parkinson's disease (PD) and Alzheimer's disease(AD) have significant gut-brain axis interaction via microbial dysbiosis. In this study, Iranian patients with PD (n = 25), ad (n = 25), and neurological disorders (ND, n = 20) were compared with healthy controls (HC, n = 20) in terms of gut microbiota abundance, short-chain fatty acid levels, and gut inflammation markers (calprotectin and zonulin). Stool and blood samples were collected from all participants and analyzed using real-time PCR, high-performance liquid chromatography, and enzyme-linked immunosorbent assay. Significant reductions in Bacteroidetes, Faecalibacterium prausnitzii, Bacteroides spp., Bifidobacterium spp., and Lactobacillus spp. were observed in PD and ad patients versus HC. Enterobacteriaceae levels were elevated in PD, ad, and ND groups, while Proteobacteria were significantly higher only in ND patients. ad patients showed reduced Actinobacteria and increased Akkermansia muciniphila compared to HC. Inflammatory markers calprotectin and zonulin were markedly elevated in all patient groups, indicating intestinal inflammation. These findings suggest that microbial dysbiosis, particularly in PD and ad, may contribute to gut barrier dysfunction and systemic inflammation. Due to sample size and methodological limitations, future studies should incorporate advanced sequencing and longitudinal designs to validate these associations and explore therapeutic implications.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic drought-tolerant bacterial community dynamics across contrasting agroclimatic zones. 不同农业气候带的内生耐旱细菌群落动态。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag013

Lopamudra Hazra, Sudipto Biswas, Kunal Kumar Saha, Sumit Chatterjee, Subhajit Dutta, Subhrangshu Mandal

{"title":"Endophytic drought-tolerant bacterial community dynamics across contrasting agroclimatic zones.","authors":"Lopamudra Hazra, Sudipto Biswas, Kunal Kumar Saha, Sumit Chatterjee, Subhajit Dutta, Subhrangshu Mandal","doi":"10.1093/lambio/ovag013","DOIUrl":"10.1093/lambio/ovag013","url":null,"abstract":"Rice (Oryza sativa) sustains more than half of the global population but is particularly vulnerable to water limitation in rainfed upland and lowland ecosystems. Endophytic microbiomes represent a sustainable strategy for improving drought resilience, yet their diversity and functional potential in traditional rice landraces remain underexplored. This study investigated endophytic bacterial communities associated with indigenous upland and lowland cultivars of West Bengal, India, using 16S rRNA amplicon sequencing, predictive functional profiling, culture-based screening, and biochemical validation. Distinct microbial assemblages revealed for diverse agro-ecosystems, with upland cultivars enriched in stress-adapted taxa such as Proteobacteria, Actinobacteria, and Firmicutes. PICRUSt2 predictions indicated higher representation of KEGG pathways associated with osmolyte biosynthesis, antioxidant defense, and compatible solute metabolism in upland microbiomes. Culture isolates, notably Enterobacter sp. KNR1 and Klebsiella sp. GBR1, exhibited strong tolerance to PEG-induced osmotic stress, producing elevated proline, glycine betaine, EPS, and IAA, alongside efficient root colonization in susceptible rice. Collectively, these findings establish indigenous upland rice as a reservoir of functionally resilient endophytes and provide a foundation for developing microbial consortia to enhance rice performance under water-limited conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary validation study of a new generation of solid phase cytometer for rapid sterility testing of pharmaceutical products. 用于药品快速无菌检测的新一代固相细胞仪的初步验证研究。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-01-14 DOI: 10.1093/lambio/ovaf140

Pauline Silberreiss, Valeria Bugatti, Silvia Scotti, Lucas Lo Giudice, Marine Schnetterle, Joseph Pierquin, Emmanuel Boutant, Valérie Andrée Geoffroy

{"title":"Primary validation study of a new generation of solid phase cytometer for rapid sterility testing of pharmaceutical products.","authors":"Pauline Silberreiss, Valeria Bugatti, Silvia Scotti, Lucas Lo Giudice, Marine Schnetterle, Joseph Pierquin, Emmanuel Boutant, Valérie Andrée Geoffroy","doi":"10.1093/lambio/ovaf140","DOIUrl":"10.1093/lambio/ovaf140","url":null,"abstract":"Ensuring the sterility of pharmaceutical products, particularly those administered parenterally, is crucial for patient safety. Conventional sterility testing methods, while reliable, require incubation periods of up to 14 days, delaying product release. Therefore, exploring alternative rapid microbiological methods is essential for improving testing efficiency and reducing the time-to-result (TTR). This study evaluates the Red One™ system, a novel rapid method utilizing solid-phase cytometry and esterase-based activity staining, which reduces the TTR to just 96 h. Our results demonstrate the non-inferiority of the Red One™ system compared to traditional culture-based techniques by assessing key performance parameters, including limit of detection, accuracy, robustness, and ruggedness for various microorganisms, including slow-growing species such as Cutibacterium acnes. Our results indicate that this alternative system is an effective method for rapid sterility testing in the pharmaceutical industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145810427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: A multiplex nested PCR assay for simultaneous detection of Corchorus golden mosaic virus and a phytoplasma in white jute (Corchorus capsularis L.). 关注表达：同时检测白黄麻（Corchorus capsularis L.）中Corchorus金花叶病毒和植物原体的多重巢式PCR方法。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-01-14 DOI: 10.1093/lambio/ovag005

引用次数: 0

Evaluation of a chromogenic agar medium for the detection of Burkholderia cepacia complex from industrial samples. 工业样品中检测洋葱伯克氏菌复合体的显色琼脂培养基的评价。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-01-14 DOI: 10.1093/lambio/ovaf141

Tomás Herrera Durigneux, Julián Mantovano, José Degrossi

{"title":"Evaluation of a chromogenic agar medium for the detection of Burkholderia cepacia complex from industrial samples.","authors":"Tomás Herrera Durigneux, Julián Mantovano, José Degrossi","doi":"10.1093/lambio/ovaf141","DOIUrl":"10.1093/lambio/ovaf141","url":null,"abstract":"Burkholderia cepacia complex (Bcc) bacteria are opportunistic pathogens that particularly affect people with cystic fibrosis and other immunosuppressed patients. These bacteria are also frequent contaminants of industrial products such as medicines and cosmetics, posing a risk to patients and raising public health concerns. Due to numerous outbreaks caused by contaminated products reported worldwide, the absence of Bcc in drugs has become mandatory under various codifications. For example, the US Pharmacopoeia recently incorporated testing for Bcc detection in non-sterile products (USP < 60 >). Detection of Bcc usually involves the use of the Burkholderia cepacia selective agar (BCSA), a selective but non-differential culture medium. In this study, we compared a recently developed chromogenic agar, CHROMagar™ B.cepacia, with BCSA for the recovery of Bcc and inhibition of non-Bcc species. We also evaluated both media for the detection of Bcc in artificially contaminated pharmaceutical and cosmetic samples. Both media exhibited similar inhibitory properties. Notably, all Bcc isolates in this study developed blue-green colonies on CHROMagar™ B.cepacia, enabling rapid and presumptive identification. These results suggest that CHROMagar™ B.cepacia could be a valuable tool for the rapid detection of Bcc in industrial samples.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: A method of direct PCR without DNA extraction for rapid detection of begomoviruses infecting jute and mesta. 关注表达：一种不提取DNA的直接PCR快速检测黄麻和间瘤begomovirus的方法。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-01-14 DOI: 10.1093/lambio/ovag003

引用次数: 0

Brewery spent yeast medium for Serratia sp. bio-beads improves Chenopodium quinoa Willd. growth in the Northern Altiplano of Bolivia. 啤酒废酵母培养基对沙雷氏菌生物微珠的改良藜麦藜。生长在玻利维亚北部高原。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-01-14 DOI: 10.1093/lambio/ovaf143

Ximena Ramirez, Virginia Gonzales, Rogelio Maydana, Mukesh Dubey, Dan Funck Jensen, Cristhian Carrasco, Magnus Karlsson, Carla Crespo

{"title":"Brewery spent yeast medium for Serratia sp. bio-beads improves Chenopodium quinoa Willd. growth in the Northern Altiplano of Bolivia.","authors":"Ximena Ramirez, Virginia Gonzales, Rogelio Maydana, Mukesh Dubey, Dan Funck Jensen, Cristhian Carrasco, Magnus Karlsson, Carla Crespo","doi":"10.1093/lambio/ovaf143","DOIUrl":"10.1093/lambio/ovaf143","url":null,"abstract":"Quinoa (Chenopodium quinoa Willd.) is a climate-resilient Andean crop with high nutritional value and strategic importance for food security in high-altitude regions. However, its productivity in low-input farming systems remains limited. This study developed scalable strategies for propagation and formulation of a Serratia sp. strain as a biofertilizer, using brewery spent yeast (BSY) as growth substrate. Microwave-assisted extraction (MAE) at 1200 W for 15 min significantly (P ≤ 0.05) enhanced soluble protein release from BSY, and MAE-treated media with a C:N ratio of 24:1 supported optimal bacterial growth. Carrageenan-based bio-bead formulations produced at 40°C with 96 g L⁻¹ carrageenan yielded the highest bacterial viability and moisture retention. In a field trial in the Bolivian Altiplano, bio-beads containing Serratia sp. applied at branching stage increased quinoa yield by up to 3.4-fold (P ≤ 0.01) compared with the control. The formulation control also substantially improved yield (2.2-fold), indicating that both the carrier matrix and bacterial inoculation contributed to growth enhancement. These findings demonstrate the potential of biofertilizer technologies based on agri-food by-product valorization to improve crop performance under extreme and resource-limited agricultural conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0