Letters in Applied Microbiology最新文献_第9页

Integrated neural network and PSO hybrid approach for production of citrulline using immobilized permeabilized Pseudomonas furukawaii. 利用固定化渗透假单胞菌（Pseudomonas furukawaii）生产瓜氨酸的集成神经网络和 PSO 混合方法。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae111

Anubhuti Kawatra, Rakhi Dhankhar, Bharti Datten, Shweta Dhankhar, Deepak Chhabra, Pooja Gulati

{"title":"Integrated neural network and PSO hybrid approach for production of citrulline using immobilized permeabilized Pseudomonas furukawaii.","authors":"Anubhuti Kawatra, Rakhi Dhankhar, Bharti Datten, Shweta Dhankhar, Deepak Chhabra, Pooja Gulati","doi":"10.1093/lambio/ovae111","DOIUrl":"10.1093/lambio/ovae111","url":null,"abstract":"In the present study, nutraceutical citrulline was produced using immobilization of permeabilized whole cells of Pseudomonas furukawaii, an efficient producer of ADI. Since arginine deiminase (ADI) is intracellularly localized, various additives such as SDS (Sodium dodecyl sulfate), Triton X-100, and EDTA (Ethylenediaminetetraacetic Acid) were used to permeabilize the cell to improve substrate accessibility and ADI activity. The maximum ADI activity was observed with 0.25 mg ml-1 biomass concentration treated with 0.5 mmol l-1 EDTA for 15 min using OFAT (One factor at a time) approach. Optimization of permeabilization conditions of P. furukawaii cells using novel neural networks and particle swarm optimization led to maximum ADI activity with 0.10 mmol l-1 EDTA and 0.30 mg ml-1 biomass. Further, the morphological characterization of immobilized cells was assessed by field emission scanning electron microscopy and FTIR (Fourier transform infrared spectroscopy). An optimal citrulline production of 1.19 mmol l-1 was achieved at 2.5% sodium alginate with 20 mmol l-1 arginine at 38°C, and 180 min of incubation. The immobilized cells retained 90.3% productivity after seven reuse cycles. Thus, the formulated immobilized whole-cell biocatalyst, with higher stability offers cost-effective methods of citrulline production.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combined therapy of meropenem-ZnO nanoparticles efficiently eliminates carbapenem-resistant Klebsiella pneumoniae biofilms, with reduced nephrotoxicity (in vitro). 美罗培南-氧化锌-纳米粒子联合疗法可有效消除耐碳青霉烯类抗生素肺炎克雷伯氏菌（CRKP）生物膜，并降低肾毒性（体外）。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae136

Alzhraa Ali Mohamed, Safaa Saed, Sara Ramadan El-Sayed, Mohamed Taha Yassin, Mohamed Gad, Eman Tartour, Hoda A Fathey, Asmaa S Taha, Asmaa H Mohamed, Fatimah Olyan Al-Otibi, Mohamed Ragab AbdelGawwad, Mohamed M Sayed Ahmed, Susan Ahmed Almalki, Mohamed Abdel-Haleem

{"title":"A combined therapy of meropenem-ZnO nanoparticles efficiently eliminates carbapenem-resistant Klebsiella pneumoniae biofilms, with reduced nephrotoxicity (in vitro).","authors":"Alzhraa Ali Mohamed, Safaa Saed, Sara Ramadan El-Sayed, Mohamed Taha Yassin, Mohamed Gad, Eman Tartour, Hoda A Fathey, Asmaa S Taha, Asmaa H Mohamed, Fatimah Olyan Al-Otibi, Mohamed Ragab AbdelGawwad, Mohamed M Sayed Ahmed, Susan Ahmed Almalki, Mohamed Abdel-Haleem","doi":"10.1093/lambio/ovae136","DOIUrl":"10.1093/lambio/ovae136","url":null,"abstract":"In response to the World Health Organization's research agenda of antimicrobial resistance in human health, this study appraised the antibacterial and antibiofilm synergistic activity of meropenem and ZnO nanoparticles (ZnO-NPs) combination against carbapenem-resistant Klebsiella pneumoniae (CRKP). The minimum inhibitory concentration (MIC) of meropenem in combination was found to be ~1/12 of its MIC alone. The results of microtiter dilution assay showed that the combination was more efficient in reducing the biofilm biomass than meropenem alone or ZnO-NPs alone. The scanning-electron-microscopy micrographs elucidated that the combination of meropenem with ZnO-NPs has significantly enhanced its competence in eradicating the preformed biofilms of CRKP strains. In addition, the relative gene expression results showed that the combination compared to the meropenem alone and ZnO-NPs alone eloquently down-regulated the expression of biofilm genes (mrkA, fimA, and ecpA). Besides, the MTT-assay demonstrated that the combination has limited cytotoxicity against Vero-cells (in vitro). Overall, this study represents an efficient safe enhancement of meropenem to tackle the growing health threat of CRKP and carbapenem-resistant Enterobacterals prevalence.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of the main contaminating materials during pig slaughter to the microbial numbers on carcasses. 生猪屠宰过程中主要污染物对屠体微生物数量的影响

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae125

Janna Tholen, Matthias Upmann

{"title":"Contribution of the main contaminating materials during pig slaughter to the microbial numbers on carcasses.","authors":"Janna Tholen, Matthias Upmann","doi":"10.1093/lambio/ovae125","DOIUrl":"10.1093/lambio/ovae125","url":null,"abstract":"During pig slaughter, contaminants such as intestinal and stomach contents, bile, tubular rail fat, and reddish foam from the respiratory tract frequently appear on carcasses, potentially compromising meat safety. This study examined the impact of these contaminants on the bacterial loads of pig carcasses, using total bacterial counts and Enterobacteriaceae counts as hygiene indicators. Examination of the substances as such showed that intestinal and stomach contents were particularly conspicuous to undermine the carcase hygiene due to total bacterial counts of ∼6.0 log10 CFU g-1 (intestinal content) and 5.5 log10 CFU g-1 (stomach content). Tubular rail fat showed varying contamination levels, from low (3.1 log10 CFU g-1) to high (6.4 log10 CFU g-1). The reddish foam had moderate contamination (4.3 log10 CFU g-1). Enterobacteriaceae levels mirrored these results at a lower level. Subsequently, a comparative study analysing bacterial levels in contaminated and in noncontaminated pork rind regions was performed. Even small amounts of intestinal and stomach contents led to significant increases in total bacterial counts of up to 3 log10 CFU cm-² and in Enterobacteriaceae counts (up to 5 log10 CFU cm-²). Other contaminants did not significantly raise bacterial levels: their total viable counts around 3.5 log10 CFU cm-² were similar to those of uncontaminated carcass areas. Nevertheless, they should be removed before further processing.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial decomposer for crop residue management in rice-wheat cropping system. 用于水稻-小麦种植系统作物残留物管理的微生物分解者

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae106

Shubham Lamba, Ranvir Singh Gill

{"title":"Microbial decomposer for crop residue management in rice-wheat cropping system.","authors":"Shubham Lamba, Ranvir Singh Gill","doi":"10.1093/lambio/ovae106","DOIUrl":"10.1093/lambio/ovae106","url":null,"abstract":"Crop residue management is vital in the rice-wheat cropping system, influencing soil health and crop productivity. This study examined the effects of organic and inorganic fertilizers and microbial decomposers on rice growth and yield. We evaluated seven treatments: 100% recommended dose fertilizer (RDF); 50% residue + 50% RDF; 50% residue + 50% RDF + Pusa decomposer; 50% residue + 50% green manuring (GM)/green leaf manuring (GLM); 50% residue + 50% GM/GLM + Pusa decomposer; residue @ 2.5 tons per acre + Pusa decomposer; residue @ 2.5 tons per acre + no Pusa decomposer; and absolute control. Results indicate that integrating organic and inorganic fertilizers with microbial decomposers positively affects rice growth and yield parameters. While adding microbial decomposer to RDF did not consistently enhance rice yield, it improved soil enzymatic properties. This suggests that the effectiveness of microbial decomposers may vary based on specific soil and crop conditions. Therefore, microbial decomposers present a promising approach to boost soil health and fertility. Further research is needed to optimize conditions for their use and systematically assess their impact on crop yields.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of anti-leptospiral antibodies using recombinant ErpY-like lipoprotein based latex agglutination test for serodiagnosis of animal leptospirosis. 使用基于重组 ErpY-Like 脂蛋白的乳胶凝集试验检测抗钩端螺旋体抗体，用于动物钩端螺旋体病的血清诊断。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae100

Kirubakaran Vinod Kumar, Prajakta Prashant Bokade, Archana Pal, Oviya Deenadayalan, Shashikumar SowjanyaKumari, Venkatappa Bharath, Bibek Ranjan Shome, Vinayagamurthy Balamurugan

{"title":"Detection of anti-leptospiral antibodies using recombinant ErpY-like lipoprotein based latex agglutination test for serodiagnosis of animal leptospirosis.","authors":"Kirubakaran Vinod Kumar, Prajakta Prashant Bokade, Archana Pal, Oviya Deenadayalan, Shashikumar SowjanyaKumari, Venkatappa Bharath, Bibek Ranjan Shome, Vinayagamurthy Balamurugan","doi":"10.1093/lambio/ovae100","DOIUrl":"10.1093/lambio/ovae100","url":null,"abstract":"Precise and timely diagnosis is essential to prevent severe outcomes of leptospirosis in humans and animals. Existing diagnostic methods face challenges and limitations, underscoring the need for novel, field-applicable screening, and diagnostic tests/assays. This study evaluates the diagnostic utility of a recombinant ErpY-like lipoprotein (rErpY-LIC11966) in a latex agglutination test (LAT) for diagnosis of animal leptospirosis. The ErpY gene sequence from Leptospira interrogans serovar Pomona, excluding the signal peptide, was amplified, cloned into the pETite vector, and expressed in Escherichia coli. The expressed rErpY (∼16 kDa) was characterized by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Western blot using Leptospira-specific standard sera. To assess the diagnostic potential of rErpY, Ni-NTA affinity-purified protein was used to sensitize latex-coated beads (0.8 µm colour beads), which were then employed in the LAT for standardization and optimization with standard positive and negative sera. For evaluation, the rErpY-LAT was tested on serum samples from 177 suspected animal cases and compared to the microscopic agglutination test. It showed a relative diagnostic sensitivity of 90.6%, a specificity of 89.1%, and an overall accuracy of 90%. This study proposes rErpY-LAT as a field testing/screening diagnostic tool for preliminary serodiagnosis of leptospirosis, highlighting the potential of recombinant protein-based assays to address current diagnostic challenges.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of high hydrostatic pressure and UV-C radiation on total aerobic bacteria in Minas Frescal cheese. 高静水压和紫外线辐射对米纳斯弗雷斯卡尔奶酪中总需氧菌的影响

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae112

Cristiane Correia Teixeira, Rafael Marques Pereira Poeys de Carvalho, Guilherme Castro Luz da Silva, Josiane Roberto Domingues, Amauri Rosenthal, Gustavo Luis de Paiva Anciens Ramos, Alice Gonçalves Martins Gonzalez

{"title":"Impact of high hydrostatic pressure and UV-C radiation on total aerobic bacteria in Minas Frescal cheese.","authors":"Cristiane Correia Teixeira, Rafael Marques Pereira Poeys de Carvalho, Guilherme Castro Luz da Silva, Josiane Roberto Domingues, Amauri Rosenthal, Gustavo Luis de Paiva Anciens Ramos, Alice Gonçalves Martins Gonzalez","doi":"10.1093/lambio/ovae112","DOIUrl":"10.1093/lambio/ovae112","url":null,"abstract":"Minas Frescal cheese (MFC) is a perishable product with high water activity and neutral pH, conditions that favor the development of microorganisms. Total aerobic bacteria (TAB) can deteriorate the cheese, negatively affecting its sensory characteristics. By controlling TAB, the shelf life of the product is extended and its quality is maintained, contributing to meeting consumer expectations. This study aimed to evaluate the individual and combined effect of technological treatments of high hydrostatic pressure (HHP) and ultraviolet C radiation (UV-C) on the TAB count present in the natural microbiota of MFC, as an alternative to control the microbiological quality of this product. After production, MFC were subjected to treatments with different levels of HHP (100-400 MPa/10 min) and UV-C (0.097-0.392 J/cm2 s-1). The combinations of HHP and UV-C doses were determined by a central composite rotational design. The model efficiently described the individual and combined effect of HHP and UV-C on TAB, demonstrating that gradually increasing HHP levels reduces TAB counts in MFC, regardless of the UV-C dose applied. This study contributes significantly to the literature by providing new insights into how these technologies can be used to improve the microbiological quality of fresh cheeses.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of Cellulomonas fimi for polysaccharide-fueled microbial fuel cells. Cellulomonas fimi 在多糖燃料微生物燃料电池方面的潜力。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae105

Naoto Hirose, Iori Kazama, Yuji Aso, Hitomi Ohara

{"title":"Potential of Cellulomonas fimi for polysaccharide-fueled microbial fuel cells.","authors":"Naoto Hirose, Iori Kazama, Yuji Aso, Hitomi Ohara","doi":"10.1093/lambio/ovae105","DOIUrl":"10.1093/lambio/ovae105","url":null,"abstract":"To generate power from various biomass using microbial fuel cells (MFCs), microorganisms with high potential are essential. Therefore, this study examined the feasibility of using Cellulomonas fimi and Shewanella oneidensis as MFCs fueled by starch, cellulose, chitin, and chitosan. To our knowledge, this is the first report of power generation using C. fimi fueled by these polysaccharides other than cellulose, furthermore the first report of S. oneidensis fueled by chitosan. No differences were observed in the power generation capacities between C. fimi and S. oneidensis when chitin and chitosan were used. However, C. fimi demonstrated effective power generation from starch and cellulose, showing a maximum current density of 17.4 mA m-2 for starch and 38.8 mA m-2 for cellulose. Shewanella oneidensis could not utilize these fuels. Power generation using C. fimi fueled by starch and cellulose produced acetic acid, lactic acid, and formic acid. However, when chitin and chitosan were used, only acetic acid was produced. These results indicate that electron transfer from C. fimi to the anode may be inefficient. To improve power generation efficiency, it may be necessary to enhance electron transfer from the cells to the anode, e.g. by adding a mediator.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Staphylococcus aureus from nasal samples of healthy pet cats. 健康宠物猫鼻腔样本中金黄色葡萄球菌的分子特征。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae108

Amel Bezzi, Kenza Antri, Mohamed Azzedine Bachtarzi, Patricia Martins-Simoes, Benjamin Youenou, Samir Gourari, Farida Nateche, Anne Tristan

{"title":"Molecular characterization of Staphylococcus aureus from nasal samples of healthy pet cats.","authors":"Amel Bezzi, Kenza Antri, Mohamed Azzedine Bachtarzi, Patricia Martins-Simoes, Benjamin Youenou, Samir Gourari, Farida Nateche, Anne Tristan","doi":"10.1093/lambio/ovae108","DOIUrl":"10.1093/lambio/ovae108","url":null,"abstract":"The objective of this study was to characterize Staphylococcus aureus isolates recovered from the nasal samples of healthy pet cats in Algiers province. A total of 138 nasal swabs were collected. Antimicrobial susceptibility was conducted using the disk-diffusion method and the VITEK-2 susceptibility system. Whole genome sequencing was performed to identify multiple-locus sequence typing, antimicrobial and virulence genes. Staphylococcus aureus isolates were detected in 23 cats. Among these, 11 were methicillin-resistant S. aureus (MRSA) (one isolate/sample). Three sequence types (ST6, ST5, and ST1) were identified in MRSA, with the predominance of ST6 (n = 7). Seven distinct STs [ST398, ST97, ST15, ST7, ST291, ST5043, and a new ST, (ST9219)] were detected in methicillin-sensitive S. aureus. All MRSA isolates harbored the mecA gene and SCCmec-type-IVa. MRSA exhibited resistance to tetracycline [n = 3/tet(L) and tet(M); n = 1/tet(K)], kanamycin-tobramycin [n = 3/ant(4')-Ia), amikacin-kanamycin (n = 1/aph(3')-IIIa], and erythromycin-clindamycin [n = 1/erm(C)]. Seven S. aureus isolates were multidrug resistant. All the isolates were negative for lukS/lukF-PV and tst-1 genes, while 20 isolates were IEC-positive. This study revealed a diversity of genetic lineages in S. aureus strains isolated from nasal samples of pet cats, including multidrug-resistant and toxigenic strains. The presence of IEC-positive S. aureus suggests possible human-animal transmission.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine Pseudomonas: diving into the waves of blue biotechnology. 海洋假单胞菌：潜入蓝色生物技术的浪潮。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae099

Anna Luiza Bauer Canellas, Marinella Silva Laport

引用次数: 0

Mitigation of chemotherapy-induced experimental intestinal mucositis through postbiotic lactate. 通过乳酸后生物素缓解化疗引起的实验性肠粘膜炎

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-11-06 DOI: 10.1093/lambio/ovae103

Isabel David Matos, Amanda Dias Borges, Luísa Martins Trindade, Maria Emília Rabelo Andrade, Gregório Grama Cavalcante, Paola Caroline Lacerda Leocádio, Jacqueline Isaura Alvarez-Leite, Geovanni Dantas Cassali, Bruno Galotti Costa, Flaviano Dos Santos Martins, Valbert Nascimento Cardoso, Simone de Vasconcelos Generoso

{"title":"Mitigation of chemotherapy-induced experimental intestinal mucositis through postbiotic lactate.","authors":"Isabel David Matos, Amanda Dias Borges, Luísa Martins Trindade, Maria Emília Rabelo Andrade, Gregório Grama Cavalcante, Paola Caroline Lacerda Leocádio, Jacqueline Isaura Alvarez-Leite, Geovanni Dantas Cassali, Bruno Galotti Costa, Flaviano Dos Santos Martins, Valbert Nascimento Cardoso, Simone de Vasconcelos Generoso","doi":"10.1093/lambio/ovae103","DOIUrl":"10.1093/lambio/ovae103","url":null,"abstract":"Postbiotic lactate modulates the immune system in inflammatory bowel diseases. However, its role in experimental intestinal mucositis (IM) has not been elucidated. This study aimed to evaluate the effects of lactate supplementation (1 and 2 × 10-1 mol/l) in a 5-fluorouracil (5-FU)-induced IM model. Male BALB/c mice (6-8 weeks old) were randomly divided into four groups: control (CTL), mucositis (MUC), mucositis with 1 × 10-1 mol/l lactate solution (MUC10), and mucositis with 2 × 10-1 mol/l lactate solution (MUC200). Lactate was administered via oral gavage for 10 days. Following the treatment period, the animals were subjected to an intraperitoneal injection of 300 mg/kg 5-FU to induce IM and were euthanized 72 h later for analysis. The MUC group presented intestinal damage with a poor histological score and decreased morphometric parameters as well as decreased mucus production and increased inflammatory infiltration and intestinal permeability compared to those of the CTL group (P < .05). However, the MUC200 group exhibited better results for the evaluated parameters than the MUC group (P < .05). Notably, the results in the MUC10 group were similar to those in the MUC group (P > .05). In conclusion, lactate supplementation attenuates mucositis-induced damage in a dose-dependent manner.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0