Letters in Applied Microbiology最新文献_第8页

First evidence of SO2-releasing bags controlling fungal growth, aflatoxins, and cyclopiazonic acid contamination in unshelled peanuts. 释放二氧化硫袋控制去壳花生真菌生长、黄曲霉毒素和环吡唑酸污染的首个证据。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag029

Carla Cervini, Vaibhav Verma, Carol Verheecke-Vaessen, Angel Medina, Naresh Magan

{"title":"First evidence of SO2-releasing bags controlling fungal growth, aflatoxins, and cyclopiazonic acid contamination in unshelled peanuts.","authors":"Carla Cervini, Vaibhav Verma, Carol Verheecke-Vaessen, Angel Medina, Naresh Magan","doi":"10.1093/lambio/ovag029","DOIUrl":"10.1093/lambio/ovag029","url":null,"abstract":"Peanuts (also known as groundnuts; Arachis hypogaea L.) are a globally significant cash crop but are highly susceptible to fungal contamination, particularly by Aspergillus section Flavi, which can contaminate the product with mycotoxins, including aflatoxins. This contamination poses serious food safety concerns, especially in low- and middle-income countries, limiting access to international markets. This study assessed the efficacy of slow-release SO2-bags compared to plastic bags (control) in reducing fungal growth and mycotoxin contamination in stored unshelled peanuts. Naturally contaminated and Aspergillus flavus-inoculated peanuts were stored at water activity (aw) levels of 0.85 and 0.95 for 15 days at 25°C. Fungal populations were monitored, and aflatoxins and cyclopiazonic acid were analysed using LC-MS/MS qTRAP. SO2-releasing bags completely inhibited fungal growth in naturally contaminated peanuts and significantly reduced A. flavus population with effectiveness dependent on water activity. Additionally, SO2-releasing bags suppressed mycotoxin production. This study demonstrates, for the first time, the effectiveness of SO2-releasing bags in preventing fungal spoilage and mycotoxin contamination in postharvest peanuts, offering a promising solution for enhancing peanut safety and quality.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities. 使用元条形码检测在基于培养的海洋沉积物细菌群落分析中快速开始的培养偏差。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag020

Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park

{"title":"Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities.","authors":"Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park","doi":"10.1093/lambio/ovag020","DOIUrl":"10.1093/lambio/ovag020","url":null,"abstract":"When cultivation-based microbiology is used to isolate strains from environmental samples, the cultured populations may not represent ecologically relevant taxa in the source community. To address this, we employed pre-cultivation metabarcoding to establish a baseline community profile and detect cultivation bias. Using time-resolved cultivation of marine sediment bacteria, we demonstrated the need for initial community characterization. Sediment-derived microbiomes were cultured in Marine Broth 2216 and analyzed using 16S rRNA gene metabarcoding at 0, 6, 12, 18, and 24 h. A rapid 10-fold reduction in alpha diversity was observed within the 6 h (from 1029 amplicon sequence variants to 34-106), with the genus Vibrio reaching near-complete dominance (>95%) from 18 to 24 h, while environmentally dominant taxa such as Acinetobacter were quickly excluded. This dramatic shift illustrates that, without baseline characterization, cultivation-induced artifacts cannot be clearly distinguished from ecologically meaningful patterns. Fast-growing generalists can quickly outcompete ecologically significant taxa, distorting isolation outcomes and hindering the recovery of functionally important microorganisms. We show that metabarcoding at 0 h can identify cultivation biases, help interpret isolation results, and suggest targeted strategies for recovering ecologically relevant taxa. This integrated approach facilitates more accurate recovery and analysis of functionally significant microbial diversity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecology and antimicrobial resistance of Campylobacter in wildlife: insights into specialist and generalist lineages and zoonotic potential. 野生动物弯曲杆菌的生态学和抗菌素耐药性：对专业和通用谱系和人畜共患潜力的见解。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag031

Manel Gharbi, Mohamed Salah Abbassi

{"title":"Ecology and antimicrobial resistance of Campylobacter in wildlife: insights into specialist and generalist lineages and zoonotic potential.","authors":"Manel Gharbi, Mohamed Salah Abbassi","doi":"10.1093/lambio/ovag031","DOIUrl":"10.1093/lambio/ovag031","url":null,"abstract":"Wildlife is a critical reservoir of Campylobacter species, particularly C. jejuni and C. coli, carrying diverse genetic lineages, virulence factors, and antimicrobial resistance (AMR) genes. Birds, especially migratory and synanthropic species, are the primary carriers, though mammals, reptiles, and other vertebrates also contribute to maintenance and dissemination. Wildlife-associated strains include both host-specific lineages and generalist clonal complexes (e.g. ST21, ST45, and ST828) capable of crossing wildlife, livestock, humans, and environmental interfaces, reflecting high zoonotic potential. Virulence factors, including motility, adhesion and invasion proteins (CadF, CiaB), and cytolethal distending toxin (CDT), facilitate colonization and survival, while efflux pumps and stress-response genes enhance persistence under antibiotic pressure. AMR is widespread, with resistance to fluoroquinolones, macrolides, tetracyclines, and multidrug phenotypes, driven by anthropogenic contamination, environmental reservoirs, and horizontal gene transfer. Key resistance determinants include gyrA mutations, tet(O), erm(B), cmeABC efflux pumps, and β-lactamases. Despite advances, knowledge gaps remain, particularly for non-avian hosts, environmental reservoirs, and resistance mechanisms. A One Health approach integrating microbiology, genomics, ecology, and epidemiology is essential to map transmission pathways, monitor emerging resistance, and guide interventions to reduce the public health impact of zoonotic and antibiotic-resistant Campylobacter.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bactericidal and anti-virulence effects of Melaleuca alternifolia essential oil against foodborne pathogens: in vitro and milk matrix assay. 互花千层精油对食源性病原菌的杀菌和抗毒作用：体外和乳基质试验。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag030

Lucília Rocha Magna, Milena Matesco Carreteiro, Gabriel José Couto, Luana de Carvalho, Matheus Henrique Nogueira, Mariana Lima Braga, André Ricardo Peron Dos Santos, Márcia Cristina Furlaneto, Regiane da Silva Gonzalez, Luciana Furlaneto Maia

{"title":"Bactericidal and anti-virulence effects of Melaleuca alternifolia essential oil against foodborne pathogens: in vitro and milk matrix assay.","authors":"Lucília Rocha Magna, Milena Matesco Carreteiro, Gabriel José Couto, Luana de Carvalho, Matheus Henrique Nogueira, Mariana Lima Braga, André Ricardo Peron Dos Santos, Márcia Cristina Furlaneto, Regiane da Silva Gonzalez, Luciana Furlaneto Maia","doi":"10.1093/lambio/ovag030","DOIUrl":"10.1093/lambio/ovag030","url":null,"abstract":"The increasing demand for natural antimicrobials has driven interest in plant-derived compounds for food safety applications. This study evaluated the antimicrobial activity of tea tree essential oil (Melaleuca alternifolia, TTO) against Escherichia coli and Salmonella spp., combining in vitro and in situ assays. Minimum inhibitory concentrations (MICs) ranged from 3.12 to 6.25 µg ml-1, and the minimum bactericidal concentrations were identical to the MICs, in media broth. Time-kill assays showed significant reductions in viable cells at 1× MIC, with complete inactivation of S. Enteritidis and S. Typhimurium within 24 h. Treatment with TTO resulted in significant changes in zeta potential and increased leakage of nucleic acids and proteins, indicating membrane destabilization. At sub-inhibitory concentrations, TTO reduced swimming motility and biofilm formation in a strain-dependent manner. In sterilized milk, MIC values were identical to those obtained in Brain Heart Infusion (BHI) for all Salmonella strains and for E. coli P2421, while E. coli ATCC 25922 exhibited a higher MIC (6.25 µg ml-1). Checkerboard assays with potassium sorbate showed an additive interaction for S. enteritidis (FICI 1.0), indifferent interactions for most strains, and antagonism for S. Typhi, resulting in a reduction in sorbate MIC from 5.0 to 2.5 mg ml-1 in selected strains.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary supplementation with chicken-derived L. rhamnosus CIQ249 protects broilers against enteropathogens by regulating intestinal homeostasis. 饲粮中添加鸡源性鼠李糖乳杆菌CIQ249可通过调节肠道内稳态来保护肉鸡免受肠道病原体的侵袭。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag024

Yimei Liu, Jiayi Xiang, Han Yu, Renjie Xu, Moxuan Mao, Jiacun Liu, Jiangfei Zhou, Jing Wang, Yigang Xu, Yuan Li

{"title":"Dietary supplementation with chicken-derived L. rhamnosus CIQ249 protects broilers against enteropathogens by regulating intestinal homeostasis.","authors":"Yimei Liu, Jiayi Xiang, Han Yu, Renjie Xu, Moxuan Mao, Jiacun Liu, Jiangfei Zhou, Jing Wang, Yigang Xu, Yuan Li","doi":"10.1093/lambio/ovag024","DOIUrl":"10.1093/lambio/ovag024","url":null,"abstract":"Lacticaseibacillus rhamnosus is commonly used to manage gastrointestinal infections in children, yet its efficacy as a probiotic feed additive for poultry against enteropathogenic bacteria remains insufficiently explored. In this study, a strain L. rhamnosus (CIQ249) was isolated from chicken intestine and evaluated for its ability to enhance intestinal homeostasis and confer resistance against enteropathogens using chicks as animal model. CIQ249 exhibited strong potential for intestinal colonization and high tolerance to gastrointestinal stress, including acid, bile, and osmotic pressure. In vitro, CIQ249 showed effective inhibitory activity against pathogenic bacteria as determined by agar well diffusion assay. During a one-month feeding trial, dietary supplementation with CIQ249 significantly improved the growth performance of broilers. In subsequent experimental challenges, oral gavage administration of CIQ249 conferred protection against E. coli O78 and S. Typhimurium. Furthermore, CIQ249 modulated intestinal immune response by increasing levels of IL-4, IL-10, IFN-γ, and sIgA, while suppressing production of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α). It also upregulated expression of tight junction proteins (ZO-1 and Claudin-1), alleviating gut damage induced by pathogenic infections. These findings indicate that CIQ249 is a promising probiotic feed additive that protects chickens from enteropathogenic bacteria by reinforcing intestinal epithelial barrier and enhancing immune homeostasis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of beneficial bacteria on growth performance and nutritional quality of white button mushroom (Agaricus bisporus ). 有益菌对双孢蘑菇生长性能和营养品质的协同效应。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag023

Reza Salehi Molkabadi, Gregory Bonito, Ali Moghadam, Zabihollah Rigi Karvandari, Fatemeh Raouf Fard

{"title":"Synergistic effects of beneficial bacteria on growth performance and nutritional quality of white button mushroom (Agaricus bisporus ).","authors":"Reza Salehi Molkabadi, Gregory Bonito, Ali Moghadam, Zabihollah Rigi Karvandari, Fatemeh Raouf Fard","doi":"10.1093/lambio/ovag023","DOIUrl":"10.1093/lambio/ovag023","url":null,"abstract":"The white button mushroom (Agaricus bisporus) is one of the most widely cultivated and consumed edible mushrooms worldwide, valued for its nutritional and health-promoting properties. Although recent studies suggest that bacteria can enhance mushroom growth and yield, the specific role of beneficial bacteria in improving productivity and bioactive compound content remains insufficiently understood. This study investigated the effects of individual and combined bacterial inoculations on A. bisporus yield, nutritional composition, and functional metabolites. Six treatments were tested in a completely randomized design, including a control (no inoculation), Bacillus subtilis ICMP 368, Pseudomonas fluorescens ICMP 3512, Pseudomonas putida ICMP 19449, Azotobacter chroococcum ICMP 15214, and co-inoculation of all four strains applied to the casing soil. Co-inoculation increased yield to 34.64 kg per 100 kg compost and biological efficiency to 125.96%, representing an 18.9% improvement over the control. Co-inoculated mushrooms exhibited higher ergosterol (3.92 mg/g dry weight), protein (3.16 g/100 g fresh weight), and trehalose (2.53 g/100 g fresh weight) contents, while the highest β-glucan level (9.86 g/100 g dry weight) was obtained with P. putida inoculation. Overall, co-inoculation of beneficial bacteria effectively enhanced both yield and nutritional quality of A. bisporus, whereas individual strains selectively improved specific bioactive compounds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dermatoprotective and anti-aging potentials of xylooligosaccharide- and glucose-fermented products by Lactiplantibacillus plantarum S61. 植物乳杆菌S61低聚木糖和葡萄糖发酵产物的皮肤保护和抗衰老潜力。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag007

Meryem Idrissi Yahyaoui, Amine Elbouzidi, Mohamed Taibi, Reda Bellaouchi, Lamyae Mehane, Sara Moumnassi, Adem Gharsallaoui, Bouchra El Guerrouj, Bassem Jaouadi, Abdelkarim Abousalham, Ennouamane Saalaoui, Abdeslam Asehraou

{"title":"Dermatoprotective and anti-aging potentials of xylooligosaccharide- and glucose-fermented products by Lactiplantibacillus plantarum S61.","authors":"Meryem Idrissi Yahyaoui, Amine Elbouzidi, Mohamed Taibi, Reda Bellaouchi, Lamyae Mehane, Sara Moumnassi, Adem Gharsallaoui, Bouchra El Guerrouj, Bassem Jaouadi, Abdelkarim Abousalham, Ennouamane Saalaoui, Abdeslam Asehraou","doi":"10.1093/lambio/ovag007","DOIUrl":"10.1093/lambio/ovag007","url":null,"abstract":"The increasing demand for multifunctional and eco-friendly dermatological products has driven the search for natural bioactive agents from microbial fermentation. In this study, the fermented products derived from (XOS) and (Glc) by Lactiplantibacillus plantarum S61, previously isolated and characterized in our laboratory, were evaluated for their dermatoprotective and cosmeceutical potential. The obtained metabolites displayed potent antimicrobial activity against major skin pathogens. Inhibition zones ranged from 12 to 14.02 mm against C. glabrata, 11 to 19.90 mm against C. albicans, and up to 20.01 mm against Staphylococcus aureus and Micrococcus luteus, with minimum inhibitory concentrations as low as 4.16%. Beyond antimicrobial effects, the fermented supernatants exhibited remarkable anti-aging activities, effectively inhibiting tyrosinase and elastase enzymes responsible for pigmentation and skin elasticity loss. The IC₅₀ values for tyrosinase inhibition were 52.49 µg mL-1 (Glc) and 26.81 µg mL-1 (XOS), while elastase inhibition reached 85.46 µg mL-1 and 35.07 µg mL-1, respectively. Moreover, the samples demonstrated photoprotective properties, with Sun Protection Factor values of 7.357 (Glc) and 11.589 (XOS), corresponding to 86.40% and 91.37% Ultraviolet B protection. These findings highlight the XOS fermented product of L. plantarum S61 as a promising multifunctional ingredient for sustainable cosmeceutical formulations combining antimicrobial, anti-aging, and photoprotective benefits.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of probiotics, prebiotics, and synbiotics on P53 and KI-67 in colorectal cancer: a scoping review. 益生菌、益生元和合成菌对结直肠癌中P53和KI-67的影响：范围综述。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag001

Sophia Pires Guimarães, Luísa Martins Trindade, Maria Luiza das Dores Rocha, Amanda Dias Borges, Maria Emilia Rabelo Andrade, Samanta Thomas Valdés, Silvia Fernandes Maurício, Rodrigo Gomes da Silva, Simone de Vasconcelos Generoso

{"title":"Effects of probiotics, prebiotics, and synbiotics on P53 and KI-67 in colorectal cancer: a scoping review.","authors":"Sophia Pires Guimarães, Luísa Martins Trindade, Maria Luiza das Dores Rocha, Amanda Dias Borges, Maria Emilia Rabelo Andrade, Samanta Thomas Valdés, Silvia Fernandes Maurício, Rodrigo Gomes da Silva, Simone de Vasconcelos Generoso","doi":"10.1093/lambio/ovag001","DOIUrl":"10.1093/lambio/ovag001","url":null,"abstract":"Changes in the gut microbiota (GM) are closely linked to colorectal cancer (CRC) development, influencing tumor cell proliferation and apoptosis. Prebiotics, probiotics, and synbiotics have been proposed as modulators of GM and potential regulators of tumor progression through key molecular markers, such as p53 and Ki-67. In this sense, a scoping review was performed following PRISMA-ScR guidelines to map available evidence on the impact of prebiotics, probiotics, and synbiotics on p53 and Ki-67 expression in CRC. Search was conducted in PubMed, SciELo, BVS/Lilacs, Embase, and Web of Science databases without date or language restrictions. Nineteen studies were included (18 preclinical and one randomized clinical trial). Ten studies assessed p53, seven evaluates Ki-67, and two examined both markers. Most preclinical studies suggested that biotic supplementation modulates apoptosis and proliferation-related pathways, often through p53 activation or Ki-67 suppression, although directionality varied across strains and models. Biotic interventions appear to influence tumor proliferation markers in CRC with strain- and model-dependent manner. Nevertheless, the evidence remains limited by heterogeneity of the study design, dosage, exposure time, and biomarker assessment. Well-designed and strain-resolved clinical trials are required to clarify whether these findings have translational and therapeutic relevance in CRC management.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145952566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prevalence and antibiotics resistance profile of Burkholderia cepacia in neonatal sepsis: a pioneer study in Kurdistan Region-Iraq. 新生儿败血症中洋葱伯克氏菌的患病率和抗生素耐药性：伊拉克库尔德斯坦地区的一项先驱研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag012

Marwa S Ibrahim, Delveen R Ibrahim, Azad A Haleem, Nizar B Yahya, Abdulrahman T Saadi, Sawsan S Abdulaziz, Revan Y Hasqyal, Khalid S Ibrahim

{"title":"The prevalence and antibiotics resistance profile of Burkholderia cepacia in neonatal sepsis: a pioneer study in Kurdistan Region-Iraq.","authors":"Marwa S Ibrahim, Delveen R Ibrahim, Azad A Haleem, Nizar B Yahya, Abdulrahman T Saadi, Sawsan S Abdulaziz, Revan Y Hasqyal, Khalid S Ibrahim","doi":"10.1093/lambio/ovag012","DOIUrl":"10.1093/lambio/ovag012","url":null,"abstract":"Burkholderia cepacia (B. cepacia) is Gram-negative, multidrug-resistant bacteria that can infect neonates and hospitalized patients, often spreading through contaminated medical devices. It causes various infections including sepsis, and it is difficult to treat due its antibiotic resistance. Over a three-year period (2021-2023), data from the ICU at Hevi Pediatric Teaching Hospital were reviewed and analyzed. Sepsis cases were identified based on clinical signs and confirmed through blood culture. B. cepacia identification and sensitivity test were confirmed using the VITEK system. Out of a total 1046 blood samples, 524 (50.1%) demonstrated positive bacterial growth, among which 142 (27.1%) identified as B. cepacia sepsis. The total neonatal cases counted for 123, 112 (91.1%) occurred in males and 11(8.9%) in females. Early-onset sepsis (EOS, ≤72 h) accounted for 49 cases (39.8%) and late-onset sepsis (LOS, > 72 h) for 74 (60.2%). The overall in-hospital mortality rate was 10.6% (13/123). Notably, B. cepacia isolates exhibited extensive multidrug resistance. Only a limited number of antimicrobials, including meropenem (3% resistance), ceftazidime (5%) and trimethoprim/sulfamethoxazole (9%) demonstrated promising results. These findings underscore an urgent need for coordinated interventions, emphasizing prudent antibiotic use and the strengthening of antimicrobial stewardship program to mitigate further resistance.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental monitoring for Listeria species in controlled atmosphere apple storage facilities. 苹果贮藏设施李斯特菌的环境监测。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-02-04 DOI: 10.1093/lambio/ovag014

Hui Zeng, De'Anthony D Morris, Avery Evans, Erik Diaz-Santiago, Teresa M Bergholz

{"title":"Environmental monitoring for Listeria species in controlled atmosphere apple storage facilities.","authors":"Hui Zeng, De'Anthony D Morris, Avery Evans, Erik Diaz-Santiago, Teresa M Bergholz","doi":"10.1093/lambio/ovag014","DOIUrl":"10.1093/lambio/ovag014","url":null,"abstract":"Studies have revealed the presence of Listeria species on food and non-food contact surfaces in apple packing facilities. However, the occurrence of Listeria in controlled atmosphere (CA) storage facilities has not been well characterized. This two-year longitudinal study assessed the prevalence, diversity, and distribution of Listeria spp. within nine CA storage rooms at three different facilities (A, B, and C), focusing on Zone 3 non food contact surfaces. A total of 304 samples were collected, and 5.9% (18) were positive for Listeria spp., predominantly on floors below condenser units. Of 18 positive sample sites, Listeria monocytogenes was the main species detected, accounting for 61.1% (11/18), followed by Listeria seeligeri at 22.2% (4/18). None of the facilities showed repeated detection of the same Listeria subtype at the same location during the two-year period. However, in Facility A, L. monocytogenes was repeatedly detected on the floor below condenser units, although the isolates belonged to different subtypes. Our findings highlight the importance of preventive measures such as environmental monitoring and sanitation programs that may mitigate Listeria spp. contamination in CA storage environments, considering the microbes ability to survive in low temperature modified atmosphere conditions conducive to long-term apple storage.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0