Letters in Applied Microbiology最新文献_第8页

Production and characterization of levans with different molecular weights synthesized by Bacillus licheniformis ZM107. 地衣芽孢杆菌ZM107合成不同分子量levans的制备及特性研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf015

Jingqi Yan, Xiangying Zhao, Liping Liu, Jiaxiang Zhang, Jing Ma

{"title":"Production and characterization of levans with different molecular weights synthesized by Bacillus licheniformis ZM107.","authors":"Jingqi Yan, Xiangying Zhao, Liping Liu, Jiaxiang Zhang, Jing Ma","doi":"10.1093/lambio/ovaf015","DOIUrl":"10.1093/lambio/ovaf015","url":null,"abstract":"Levan, a fructan-type polysaccharide with diverse applications in food, pharmaceuticals, and biotechnology, has garnered significant attention for its functional properties, such as prebiotic, immunomodulatory, antioxidant, and antimicrobial activities. In this study, the effects of fermentation temperature, sucrose concentration, and incubation time on the molecular weight of levan produced by Bacillus licheniformis fermentation were investigated. The results showed that as the temperature (37°C-50°C) and sucrose concentration (300-500 g l-1) increased, the molecular weight of levan produced by the strain during fermentation decreased. At the same time, the molecular weight initially increased and then decreased with longer culture times, indicating that levan within a specific size range could be produced by controlling the fermentation conditions. In addition, the properties and characteristics of levan produced by fermentation at two molecular weights (HML and LML) were compared. The results showed that molecular weight significantly affected the micromorphology, thermal behavior, rheological properties, and prebiotic activity of levan. Therefore, this study demonstrated that Bacillus licheniformis SFLV-ZM107 can produce low molecular weight levan, and its molecular weight can be controlled through fermentation conditions. Consequently, the properties and functions of levan can be influenced, allowing the production of levans with the desired characteristics.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-silico study of molecular adaptations in halophilic Cas9. 嗜盐Cas9分子适应性的计算机研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf006

Anisha Debnath, Aveepsa Sengupta, Sujata Rudrapal, Ashutosh Kumar, Mamta Rani

{"title":"In-silico study of molecular adaptations in halophilic Cas9.","authors":"Anisha Debnath, Aveepsa Sengupta, Sujata Rudrapal, Ashutosh Kumar, Mamta Rani","doi":"10.1093/lambio/ovaf006","DOIUrl":"10.1093/lambio/ovaf006","url":null,"abstract":"This study explores the structural adaptations of the CRISPR-Cas9 system in halophilic bacteria, focusing on Cas9 protein of halophilic bacterium Salicibibacter cibi. Protein sequences were analyzed using different tools such as ExPASy ProtParam for different physicochemical properties, Predictor of Natural Disordered Regions web server for disordered regions, and InterPro server and WebLogo for domains. Protein structures were generated using the AlphaFold database, and the quality of the modelled structure was checked through PROCHECK. The protein surface's amino acids and electrostatic potential were visualized using PyMOL, APBS server, and UCSF chimera. Comparative analysis revealed that halophilic Cas9 proteins possess a higher abundance of acidic residues, resulting in enhanced stability and hydration in saline conditions; halophilic Cas9 proteins also shows higher intrinsically disordered regions. Electrostatic potential maps confirmed that S. cibi Cas9 proteins maintain a highly negative surface charge, crucial for adaptation to salt-rich environments. These findings provide insights into the molecular mechanisms driving the structural and functional adaptations of Cas9 in salty environment, highlighting its potential applications in genome editing-based biotechnological approaches in extreme conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of the environmental spread of Salmonella enterica serovars through water in Africa. 非洲肠道沙门氏菌血清型经水环境传播的研究进展。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf005

Akeem Ganiyu Rabiu, Oyinkansola Fadiji, Ayodele Timilehin Adesoji, Simiat Olanike Jimoh, Sohyun Cho

{"title":"A review of the environmental spread of Salmonella enterica serovars through water in Africa.","authors":"Akeem Ganiyu Rabiu, Oyinkansola Fadiji, Ayodele Timilehin Adesoji, Simiat Olanike Jimoh, Sohyun Cho","doi":"10.1093/lambio/ovaf005","DOIUrl":"10.1093/lambio/ovaf005","url":null,"abstract":"Salmonella is an enteric pathogenic bacterium in mammals that thrives in sewage, soil, and aquatic environments because of its wide ecological adaptability. The spread of Salmonella infection is associated with a lack of clean water, poor hygiene, and poor sanitation in developing countries. However, the input of Salmonella-contaminated surface water and groundwater in the environmental dissemination of antimicrobial-resistant Salmonella is obscure outside developed countries. This study aimed to assess literature that described Salmonella from groundwater, surface water, and treated water in Africa and profiled the bacteria for antimicrobial resistance (AMR). We seek to highlight the pitfalls in S. enterica identification schemes and determine the entry routes of S. enterica into surface water and groundwater to comprehend the spread patterns of S. enterica through the water ecosystem. Salmonella was frequently recovered not only from groundwater but also from unprotected springs and river water in Africa. However, the insufficiency of standard methods limited the speciation of the bacteria and only a few studies determined the isolates' AMR patterns. Integrating the Salmonella surveillance in environmental and treated water into the global Salmonella surveillance can assist in controlling typhoid fever spread and generally reduce the burden of waterborne infections in Africa.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hospital environment as reservoir of Pseudomonas aeruginosa in human cases: a molecular epidemiology investigation in a hospital setting in central Italy. 医院环境作为人类病例中铜绿假单胞菌的蓄水池：意大利中部医院环境中的分子流行病学调查。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf019

Adele Lombardi, Manuela Tamburro, Michela Lucia Sammarco, Giancarlo Ripabelli

{"title":"Hospital environment as reservoir of Pseudomonas aeruginosa in human cases: a molecular epidemiology investigation in a hospital setting in central Italy.","authors":"Adele Lombardi, Manuela Tamburro, Michela Lucia Sammarco, Giancarlo Ripabelli","doi":"10.1093/lambio/ovaf019","DOIUrl":"10.1093/lambio/ovaf019","url":null,"abstract":"Pseudomonas aeruginosa is one of the main causes of hospital infections that are difficult to manage because of multidrug resistance (MDR). The aim of this study was to describe a molecular investigation on 19 clinical and 14 isolates from the environment in the Hub hospital of the Molise region, central Italy. Antimicrobial susceptibility was evaluated using BD Phoenix™ Automated Microbiology System. Pulsed-field gel electrophoresis (PFGE) with SpeI, enterobacterial repetitive intergenic consensus (ERIC-PCR), and random amplified polymorphic DNA-PCR were performed for genotyping. All 33 P. aeruginosa showed MDR phenotype. PFGE had 0.99 discriminatory power, underlining high heterogeneity among the strains. The genetic relatedness between two human isolates (H12 and H15) from neonatal intensive care (NICU) and one environmental strain (E1) collected from siphon of sink in the delivery room was noticeable, as well as between one strain from faucet in NICU (E2) and siphon sink (E5) from delivery room. The link between H12, H15, and E1 strains was corroborated by ERIC-PCR showing epidemiological concordance, although with a lower discriminatory power. The study findings strengthened the critical correlation between clinical P. aeruginosa and environment, according to previous molecular surveys on outbreaks occurred in Italy.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genomic characterization of Levilactobacillus brevis YT108: a potential probiotic strain capable of metabolizing xylo-oligosaccharides. 短乳酸杆菌YT108的表型和基因组特征：一种能够代谢低聚木糖的潜在益生菌菌株。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf014

Yang Chen, Jun Zhou, Xin Li, Xin-Yao Li, Shi-Xiu Qiu, Zhen-Ying Xu, Jian-Xia Yang, Yuan-Ting Zhu, Xin-Rui Zhang, Lang Yan

{"title":"Phenotypic and genomic characterization of Levilactobacillus brevis YT108: a potential probiotic strain capable of metabolizing xylo-oligosaccharides.","authors":"Yang Chen, Jun Zhou, Xin Li, Xin-Yao Li, Shi-Xiu Qiu, Zhen-Ying Xu, Jian-Xia Yang, Yuan-Ting Zhu, Xin-Rui Zhang, Lang Yan","doi":"10.1093/lambio/ovaf014","DOIUrl":"10.1093/lambio/ovaf014","url":null,"abstract":"Levilactobacillus brevis YT108, identified for its ability to metabolize prebiotic xylo-oligosaccharides (XOS), emerges as a candidate for probiotic use in synbiotic food formulations. This study aimed to investigate the metabolic and genomic traits associated with XOS metabolism in YT108 and to assess its probiotic attributes through whole genome sequencing and in vitro assays. Strain YT108 exhibited robust growth kinetics on XOS as the sole carbon source, with a growth profile comparable to that on glucose, achieving a pH reduction to 4.68 and an OD600nm of 1.603 after 48 h. Three key gene clusters (xylCDEPFRT, xylHTG, and xylABT) and key enzymes (1,4-β-xylosidase) were identified as potentially involved in XOS metabolism. In vitro assays confirmed the strain's remarkable physiological properties, including tolerance to acid, bile, heat, and NaCl, as well as resistance to simulated gastrointestinal juices and antioxidant capacity. Furthermore, strain YT108 was sensitive to five commonly used antibiotics and lacked transferable resistance genes. Taken together, these results highlight the potential of L. brevis YT108 as a probiotic candidate with beneficial traits for XOS utilization, suggesting its promising application in the formulation of next-generation synbiotic products.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and antimicrobial resistance profiles of Salmonella enterica recovered from house fly intestinal tracts and environments of selected broiler farms in Texas. 德克萨斯州部分肉鸡养殖场家蝇肠道及环境中肠炎沙门氏菌的检测及耐药性分析。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf007

Kirsten M Zwally, Elizabeth Holda, Isaac Perez, Phillip E Kaufman, Brandon Lyons, Giridhar Athrey, T Matthew Taylor

{"title":"Detection and antimicrobial resistance profiles of Salmonella enterica recovered from house fly intestinal tracts and environments of selected broiler farms in Texas.","authors":"Kirsten M Zwally, Elizabeth Holda, Isaac Perez, Phillip E Kaufman, Brandon Lyons, Giridhar Athrey, T Matthew Taylor","doi":"10.1093/lambio/ovaf007","DOIUrl":"10.1093/lambio/ovaf007","url":null,"abstract":"The entry of drug-resistant Salmonella enterica into the food supply is a challenge to public health and food safety. One emerging concern is the role of synanthropic insects for moving microbial pathogens throughout poultry production systems, where insects commonly thrive. We investigated the presence and phenotypic antimicrobial susceptibility of S. enterica from insect and environmental samples from broiler farms. Insects were collected throughout the broiler house and adjacent compost barn. Environmental samples (poultry feed, drinking water, fresh litter, and feces) were collected simultaneously (n = 80). Insect gastrointestinal tracts were dissected and pooled (n = 57). Recovered Salmonella isolates were serotyped and subjected to antimicrobial susceptibility testing against 14 medically important antimicrobials. Overall, six isolates were recovered from 137 total samples (4.4%): 3.5% (2/57) from adult house flies (Musca domestica), 15% (3/20) from poultry feed, and 4.8% (1/21) from litter. Salmonella Montevideo (16.7%; 1/6), Typhimurium (33.3%; 2/6), and Kentucky (50%, 3/6) were identified. All but one Salmonella isolate (83.3%; 5/6) demonstrated resistance to at least one antimicrobial. Further research should investigate movement patterns between broiler operations and food processing facilities to establish efficient biosecurity measures to prevent any instances of foodborne pathogen transmission into human food systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructed wetland-microbial fuel cell (CW-MFC) mediated bio-electrodegradation of azo dyes from textile wastewater. 人工湿地-微生物燃料电池（CW-MFC）介导纺织废水中偶氮染料的生物电降解。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf010

Nikita Kundu, Smriti Yadav, Ananya Bhattacharya, G K Aseri, Neelam Jain

{"title":"Constructed wetland-microbial fuel cell (CW-MFC) mediated bio-electrodegradation of azo dyes from textile wastewater.","authors":"Nikita Kundu, Smriti Yadav, Ananya Bhattacharya, G K Aseri, Neelam Jain","doi":"10.1093/lambio/ovaf010","DOIUrl":"10.1093/lambio/ovaf010","url":null,"abstract":"Azo dyes constitute 60%-70% of commercially used dyes and are complex, carcinogenic, and mutagenic pollutants that negatively impact soil composition, water bodies, flora, and fauna. Conventional azo dye degradation techniques have drawbacks such as high production and maintenance costs, use of hazardous chemicals, membrane clogging, and sludge generation. Constructed wetland-microbial fuel cells (CW-MFCs) offer a promising sustainable approach for the bio-electrodegradation of azo dyes from textile wastewater. CW-MFCs harness the phytodegradation capabilities of wetland plants like Azolla, water hyacinth, and Ipomoea, along with microalgae such as Nostoc, Oscillatoria, Chlorella, and Anabaena, to break down azo dyes into aromatic amines. These intermediates are then reduced to CO2 and H2O by microalgae in the fuel cells while simultaneously generating electricity. CW-MFCs offer advantages including low cost, sustainability, and use of renewable energy. The valorization of the resulting algal and plant biomass further enhances the sustainability of this approach, as it can be used for biofuel production, nutraceuticals, pharmaceuticals, and bio-composting. Implementing CW-MFCs as a tertiary treatment step in textile industries aligns with the circular economy concept and contributes to achieving several sustainable development goals.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common mycorrhizal networks enhance growth and nutrient uptake in non-mycorrhizal Chenopodium album via Parthenium hysterophorus. 普通菌根网络通过帕特嫩草促进非菌根茄子的生长和营养吸收。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf009

Ambika Chauhan, Mankeet Kaur, Meenakshi Singh, Pradeep Kumar Singh

{"title":"Common mycorrhizal networks enhance growth and nutrient uptake in non-mycorrhizal Chenopodium album via Parthenium hysterophorus.","authors":"Ambika Chauhan, Mankeet Kaur, Meenakshi Singh, Pradeep Kumar Singh","doi":"10.1093/lambio/ovaf009","DOIUrl":"10.1093/lambio/ovaf009","url":null,"abstract":"Common mycorrhizal networks (CMNs) facilitate nutrient transfer between plants, but their role in supporting non-mycorrhizal species remains largely unexplored. This study investigates the effect of CMNs on the growth and nutrient uptake of the non-mycorrhizal plant Chenopodium album in association with the mycorrhizal plant Parthenium hysterophorus. The treatments included C. album alone, C. album grown with P. hysterophorus to allow CMN formation, and C. album treated with fungicide to inhibit mycorrhizal activity. Results showed that CMN treatment significantly enhanced the plant growth and uptake of nutrient content (N and P) in C. album compared to the control. There was no mycorrhizal colonization in C. album, while high colonization in P. hysterophorus suggested that the enhanced growth in C. album was due to nutrient uptake transfer through the CMNs. The fungicide treatment resulted in reduced growth and uptake of nutrient content, providing further evidence that CMNs and mycorrhizal associations of mycorrhizal plants enhanced the growth of C. album. These findings provide the first evidence that CMNs can enhance the growth and nutrient uptake of non-mycorrhizal plants through associations with mycorrhizal partners.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the bacterial communities in the sediment-water interface across different salinities of Pacific White shrimp, Penaeus vannamei, by metabarcoding. 利用元条形码技术研究太平洋白对虾（Penaeus vanamei）不同盐度下沉积物水界面的细菌群落。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf020

Natarajan Lalitha, Vinaya Kumar Katneni, Ashok Kumar Jangam, Panjan Nathamuni Suganya, Suvana Sukumaran, Moturi Muralidhar

{"title":"Insight into the bacterial communities in the sediment-water interface across different salinities of Pacific White shrimp, Penaeus vannamei, by metabarcoding.","authors":"Natarajan Lalitha, Vinaya Kumar Katneni, Ashok Kumar Jangam, Panjan Nathamuni Suganya, Suvana Sukumaran, Moturi Muralidhar","doi":"10.1093/lambio/ovaf020","DOIUrl":"10.1093/lambio/ovaf020","url":null,"abstract":"Microbes play an important role in the food chain by metabolizing organic matter, cycling nutrients, and maintaining a dynamic equilibrium among organisms in water and sediment. The objective is to study the fluctuating taxonomic microbial diversity profile in the sediment-water interface at different days of culture (DOC) of Penaeus vannamei in varying salinities using the Illumina MiSeq platform. Sediment samples were collected in Tamil Nadu, India, from low-saline, brackish water, and high-saline ponds at 30, 60, and 90 DOC. Bacterial richness and diversity in species were high in low-saline ponds. Beta-diversity variation indicated more differences in bacterial composition in high- and low-saline ponds. The predominant phyla observed were Proteobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, Firmicutes, and Cyanobacteria. High-saline ponds accounted for more nitrification bacterial communities, sulfur-reducing bacterial communities, sulfur-oxidizing bacterial communities, and high redox potential, whereas denitrification bacterial communities were high in brackish water ponds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the preparation of a spiny spore high-concentrated Aspergillus brasiliensis suspension. 刺孢子高浓度巴西曲霉悬浮液制备工艺优化。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae141

Federica Pisa, Mirella Gionta, Antonio Biasi, Maura Pizzuti, Jodi R Alt, Imma Zampaglione

{"title":"Optimization of the preparation of a spiny spore high-concentrated Aspergillus brasiliensis suspension.","authors":"Federica Pisa, Mirella Gionta, Antonio Biasi, Maura Pizzuti, Jodi R Alt, Imma Zampaglione","doi":"10.1093/lambio/ovae141","DOIUrl":"10.1093/lambio/ovae141","url":null,"abstract":"This work aimed to improve some steps of the existing guidelines of the European Standards to obtain an Aspergillus brasiliensis ATCC 16404 spore suspension with >75% spiny spores without mycelia and a concentration of at least 1.5 × 108 CFU ml-1. Several manufacturers' combinations of \"strain/medium\" were assessed in terms of yield of spiny spores. Criteria to establish success included (i) the growth over different times of incubation (4, 7, and 10 days); (ii) the performance of two different filtration devices (fritted filters and cell strainers); and (iii) the fungal spores susceptibility after 4 days of incubation versus 7 days of incubation against a peracetic acid-based disinfectant. The best combination was \"Microbiologics strain/Oxoid MEA\" with 88.33% of spiny spores already after 4 days, and no statistical differences (P < .05) in terms of growth in the three time points were highlighted (from 1.85 × 108 CFU ml-1 to 2.21 × 108 CFU ml-1). Cell strainers gave a spore suspension much more concentrated (3.31 times) compared to fritted filters. Furthermore, the 4-day-old spore suspension showed the same susceptibility to disinfection when compared to a suspension whose culture was incubated for 7 days.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0