Letters in Applied Microbiology最新文献_第8页

Preparation protocol for epifluorescence microscopy when working close to the detection limit. 接近检出限时的荧光显微镜制备规程。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf026

Rebecca McCerery, Lewis Hall, Jithin Omanakuttan, Hui Zhi Chai, James Lawrence, John Woodward, David A Pearce

{"title":"Preparation protocol for epifluorescence microscopy when working close to the detection limit.","authors":"Rebecca McCerery, Lewis Hall, Jithin Omanakuttan, Hui Zhi Chai, James Lawrence, John Woodward, David A Pearce","doi":"10.1093/lambio/ovaf026","DOIUrl":"10.1093/lambio/ovaf026","url":null,"abstract":"Working with low density, low biomass material can be challenging, especially when working near the detection limit. Although background contamination is a universal consideration in microbiological research, its impact is increased when the cells under assessment approach the same concentration as the background contamination. The aim of this work was to identify and remove laboratory sources of background contamination in the cell mounting process for epifluorescence microscopy to improve the reliability of cell counting for low biomass samples. Microscope slides and coverslips were assessed before and after autoclaving, washing with detergent and rinsing with ethanol solution. The solutions used in sample mounting; 4',6-diamidino-2-phenylindole, phosphate buffered saline, and immersion oil, were tested before and after autoclaving as well as both single and triple filtering with a 0.2 µm membrane filter. Using a combination of detergent and ethanol rinses of glassware and triple filtering of all solutions, we were able to reduce the background contamination by almost two orders of magnitude, down from 1 × 104(±4.3 × 103) cells to 302(±312) cells per filter paper. This method was then validated with low biomass glacial sediment samples from Renardbreen, Svalbard, with cell concentrations of 1.8 × 105(±2.9 × 104) cells g-1, close to the reported detection limit of epifluorescence microscopy.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a method to test the efficacy of different sanitizers for use in rubber footbaths as applied to dairy manufacturing. 设计了一种方法来测试乳制品生产中橡胶足浴中使用的不同消毒剂的功效。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf035

Denise Lindsay, Vanessa Thompson, Megan Harrigan

引用次数: 0

Learn from artificial intelligence: the pursuit of objectivity. 向人工智能学习：追求客观。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf021

Fengyi Wang, Angeliki Marouli, Pisit Charoenwongwatthana, Chien-Yi Chang

{"title":"Learn from artificial intelligence: the pursuit of objectivity.","authors":"Fengyi Wang, Angeliki Marouli, Pisit Charoenwongwatthana, Chien-Yi Chang","doi":"10.1093/lambio/ovaf021","DOIUrl":"10.1093/lambio/ovaf021","url":null,"abstract":"Humans continuously face threats from emerging novel pathogens and antimicrobial resistant bacteria or fungi, which requires urgently and efficient solutions. Alternatively, microbes also produce compounds or chemicals highly valuable to humans of which require continuous refinement and improvement of yields. Artificial intelligence (AI) is a promising tool to search for solutions combatting against diseases and facilitating productivity underpinned by robust research providing accurate information. However, the extent of AI credibility is yet to be fully understood. In terms of human bias, AI could arguably act as a means of ensuring scientific objectivity to increase accuracy and precision, however, whether this is possible or not has not been fully discussed. Human bias and error can be introduced at any step of the research process, including conducting experiments and data processing, through to influencing clinical applications. Despite AI's contribution to advancing knowledge, the question remains, is AI able to achieve objectivity in microbiological research? Here, the benefits, drawbacks, and responsibilities of AI utilization in microbiological research and clinical applications were discussed.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between pathogenic bacteria and different stages of colorectal cancer. 致病菌与结直肠癌不同分期的关系。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf017

Javad Nezhadi, Hossein Samadi Kafil, Mohammad Sadrkabir, Farshad Mahdavi, Seyed Yaghoub Moaddab, Roghayeh Nouri, Yalda Mohammadzadeh-Asl, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee

{"title":"The relationship between pathogenic bacteria and different stages of colorectal cancer.","authors":"Javad Nezhadi, Hossein Samadi Kafil, Mohammad Sadrkabir, Farshad Mahdavi, Seyed Yaghoub Moaddab, Roghayeh Nouri, Yalda Mohammadzadeh-Asl, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee","doi":"10.1093/lambio/ovaf017","DOIUrl":"10.1093/lambio/ovaf017","url":null,"abstract":"Colorectal cancer (CRC) involves uncontrolled cell growth in the colon and rectum. This study aims to explore the prevalence of key pathogenic bacteria and their role in the progression of CRC, focusing on microbial dysbiosis. This study analyzed 52 stool and tissue samples through polymerase chain reaction (PCR), real-time PCR, and bioinformatics to identify associations between pathogenic bacteria and CRC progression. PCR results revealed a significant association between the Bacteroides fragilis toxin (bft) gene and CRC progression (P = 0.001, r = 0.570). Furthermore, Real-time PCR showed significant differences in the frequency of pks+Escherichia coli in CRC stages 1 (P = 0.03), 2 (P = 0.004), and 3 (P = 0.0002) compared to the control group. Additionally, the frequency of Fusobacterium nucleatum in stage 3 CRC patients was significantly higher than in the control group (P = 0.004) and stage 1 patients (P = 0.01). Furthermore, Streptococcus gallolyticus showed similar significant differences in stage 3 patients (P = 0.004). Bioinformatics analyses using KEGG, Reactome, STRING, and dbSNP highlighted bacteria's roles in colorectal carcinogenesis, emphasizing the need for early identification and management in CRC treatment and prevention strategies. Finally, due to the limitations of the study, the use of more advanced methods and the validation of results through more reliable techniques are essential for future research.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of resistant coliform bacteria in the intestine of pigs following flock versus individual treatment with neomycin against post-weaning diarrhoae or amoxicillin against umbilical infection. 猪群后肠道中耐药大肠菌群的选择与单独使用新霉素治疗断奶后腹泻或阿莫西林治疗脐部感染的比较

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf008

Egle Kudirkiene, Esben Østergaard Eriksen, Gang Liu, Anja Ejlersgård Christensen, Marianne Viuf Agerlin, Inge Larsen, Nicolai Rosager Weber, Ken Steen Pedersen, Jens Peter Nielsen, John Elmerdahl Olsen

{"title":"Selection of resistant coliform bacteria in the intestine of pigs following flock versus individual treatment with neomycin against post-weaning diarrhoae or amoxicillin against umbilical infection.","authors":"Egle Kudirkiene, Esben Østergaard Eriksen, Gang Liu, Anja Ejlersgård Christensen, Marianne Viuf Agerlin, Inge Larsen, Nicolai Rosager Weber, Ken Steen Pedersen, Jens Peter Nielsen, John Elmerdahl Olsen","doi":"10.1093/lambio/ovaf008","DOIUrl":"10.1093/lambio/ovaf008","url":null,"abstract":"The aim of this study was to investigate the effect of avoiding flock treatment on resistance levels in the intestine of pigs. To investigate this, studies, each in two pig herds, quantified resistant coliforms by culture method in pigs treated as flock or as individual animal treatments orally with neomycin against post-weaning diarrhea (PWD) or intramuscularly with amoxicillin to prevent umbilical infection. Individual oral treatment against PWD did not lead to a lower number or proportion of resistant coliforms compared to flock treated pigs in any of the two herds under study, despite reduction of treatment incidences to 32% and 35% of pigs. After intramuscular treatment against umbilical infection, fewer resistant bacteria were seen in individually treated piglets in a herd with low treatment incidence (33%), while no reduction of resistant coliform bacteria compared to flock treatment was observed in the other herd with higher treatment incidence (86%). Thus, individual animal treatment reduced the amount of antimicrobial used, but concurrent reduction in resistant coliform bacteria was not always observed.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater. 苏米林坝浮游植物培养及其对水产养殖废水的生物修复能力研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf013

Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling

{"title":"Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater.","authors":"Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling","doi":"10.1093/lambio/ovaf013","DOIUrl":"10.1093/lambio/ovaf013","url":null,"abstract":"Sumiling Dam in Sarrat, Ilocos Norte, is home to diverse phytoplankton species. This study aims to isolate and identify phytoplankton species, optimize the cultivation of one phytoplankton under varying light, pH, and temperature conditions, and assess its bioremediation capacity in aquaculture wastewater. Three stations were set up at the site, each with three sampling points spaced 3-5 m apart. From each point, 300 ml of water was collected using standard procedures and transported to the lab for phytoplankton identification. Samples were incubated in algae culture broth for 14 days, streak-plated for isolation, and cultured under light. Microscope examination was used to characterize phytoplankton species, and the identification was verified by a professional. Ten phytoplankton species were identified, with Haematococcus sp. selected for wastewater bioremediation due to its optimal growth under specific conditions (81 µmol m-2 s-1, pH 9, 25°C ± 2°C). Experiments showed that Haematococcus sp. significantly reduced nitrogen and phosphorus in wastewater over 7-14 days, with Treatment 3 (1000 mg/l) achieving the highest growth, chlorophyll, and nitrogen reduction, while Treatment 2 (750 mg/l) excelled in phosphorus removal. These results underscore phytoplankton's potential for sustainable, large-scale wastewater bioremediation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model. 从尿毒症患者分离的hutiherbaspirillum的特性：在mellonella模型中的毒力和抗菌功效。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf012

Yuchun Liu, Rui Zhu, Dongmei Liu, Yue Hu, Qing Xia, Xinwei Liu, Chunxia Wang, Yongwei Li

{"title":"The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model.","authors":"Yuchun Liu, Rui Zhu, Dongmei Liu, Yue Hu, Qing Xia, Xinwei Liu, Chunxia Wang, Yongwei Li","doi":"10.1093/lambio/ovaf012","DOIUrl":"10.1093/lambio/ovaf012","url":null,"abstract":"Herbaspirillum species are typically isolated from plants and are known for their role in crop nitrogen fixation. Recently, they have been found to colonize humans and cause infections. This study aimed to identify and characterize two Herbaspirillum huttiense strains (CLJ01 and CLJ02) isolated from the blood of a uremic patient, with a focus on evaluating their pathogenicity and antibiotic efficacy. The strains were identified using the VITEK2 system, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry , and 16S rRNA gene sequencing, confirming their identity as H. huttiense. High-throughput sequencing further revealed the presence of the resistance gene arnA, which confers resistance to polymyxin. For the first time, the pathogenicity of H. huttiense was assessed using a Galleria mellonella infection model. The results demonstrated a concentration-dependent virulence, with CLJ01 exhibiting slightly higher pathogenicity. Additionally, meropenem showed significant antimicrobial efficacy in the G. mellonella infection model, particularly under conditions of high bacterial load, indicating strong therapeutic potential. In conclusion, this study provides experimental evidence supporting the correct diagnosis and treatment of H. huttiense infections. Furthermore, the findings underscore the importance of accurately identifying rare pathogens in clinical settings for effective treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Enrichment of laccase production by Phoma herbarum isolate KU4 under solid-state fermentation by optimizing RSM coefficients using genetic algorithm. 修正：利用遗传算法优化RSM系数，在固态发酵条件下提高了Phoma herbarum KU4的漆酶产量。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf018

引用次数: 0

Urease-catalyzed microbial and enzymatic carbonate precipitation for eco-friendly heavy metal remediation. 脲酶催化微生物和酶促碳酸盐沉淀法用于生态友好型重金属修复。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf022

Adharsh Rajasekar, Armstrong Ighodalo Omoregie, Kan Fock Kui

{"title":"Urease-catalyzed microbial and enzymatic carbonate precipitation for eco-friendly heavy metal remediation.","authors":"Adharsh Rajasekar, Armstrong Ighodalo Omoregie, Kan Fock Kui","doi":"10.1093/lambio/ovaf022","DOIUrl":"10.1093/lambio/ovaf022","url":null,"abstract":"Heavy metal contamination significantly threatens environmental and public health, necessitating effective and sustainable remediation technologies. This review explores two innovative bioremediation techniques: microbially induced calcium carbonate precipitation (MICP) and enzyme-induced calcium carbonate precipitation (EICP). Both techniques show promise for immobilizing heavy metals in laboratory and field settings. MICP utilizes the metabolic activity of ureolytic microorganisms to precipitate calcium carbonate, sequestering heavy metals such as lead, cadmium, and arsenic as stable metal-carbonate complexes. EICP, on the other hand, employs urease enzymes to catalyze calcium carbonate precipitation, offering greater control over reaction conditions and higher efficiency in environments unfavorable to microbial activity. This mini-review compares the mechanisms of MICP and EICP, focusing on factors influencing their performance, including enzyme or microbial activity, pH, temperature, and nutrient availability. Case studies illustrate their success in sequestering heavy metals, emphasizing their practical applications and environmental benefits. A comparative analysis highlights the strengths and limitations of MICP and EICP regarding cost, scalability, and challenges. This review synthesizes research to support the advancement of MICP and EICP as sustainable solutions for mitigating heavy metal contamination.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a quantitative SYBR Green real-time PCR and microscopic slide culture for in vitro enumeration of Mycoplasma hyopneumoniae. SYBR绿色实时荧光定量PCR和载玻片培养法体外计数肺炎支原体的建立。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf011

Raziallah Jafari Jozani, Mauida F Hasoon Al Khallawi, Majed H Mohammed, Hanh Thi Hong Nguyen, Darren J Trott, Kiro Petrovski, Farhid Hemmatzadeh

{"title":"Development of a quantitative SYBR Green real-time PCR and microscopic slide culture for in vitro enumeration of Mycoplasma hyopneumoniae.","authors":"Raziallah Jafari Jozani, Mauida F Hasoon Al Khallawi, Majed H Mohammed, Hanh Thi Hong Nguyen, Darren J Trott, Kiro Petrovski, Farhid Hemmatzadeh","doi":"10.1093/lambio/ovaf011","DOIUrl":"10.1093/lambio/ovaf011","url":null,"abstract":"Mycoplasma hyopneumoniae's slow growth makes enumeration challenging using the traditional colony-forming unit (CFU) method. We introduced an innovative CFU-slide culture (CFU-SC) approach to address this issue. CFU-SC allows accurate counting of M. hyopneumoniae microcolonies, ranging from 7 to 50 µm, using high magnification. While comparing the accuracy of enumeration among CFU, CFU-SC, quantitative polymerase chain reaction (qPCR), and colour change unit (CCU), we found a robust positive correlation between qPCR and CFU-SC with colourCCU method for quantifying M. hyopneumoniae. Newly isolated strains exhibited the lowest Pearson correlation coefficient (r = 0.52) when comparing CFU and real-time PCR, while the highest coefficient (r = 0.86) was observed for the type strain J and new isolates using real-time PCR and CFU-SC. A consensus growth pattern was observed among all quantification methods, except CFU, between newly isolated strains and the type strain J. Notably, new isolates of M. hyopneumoniae showed no senescence phase after 228 h of cultivation, differing from the type strain J. The disparity in growth rate and pattern between new isolates and the type strain J is evident in the smaller agar microcolonies (7-10 µm) of the isolates, contrasting with the larger colonies (100-200 µm) of type strain J.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0