Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater.

IF 2.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling
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Abstract

Sumiling Dam in Sarrat, Ilocos Norte, is home to diverse phytoplankton species. This study aims to isolate and identify phytoplankton species, optimize the cultivation of one phytoplankton under varying light, pH, and temperature conditions, and assess its bioremediation capacity in aquaculture wastewater. Three stations were set up at the site, each with three sampling points spaced 3-5 m apart. From each point, 300 ml of water was collected using standard procedures and transported to the lab for phytoplankton identification. Samples were incubated in algae culture broth for 14 days, streak-plated for isolation, and cultured under light. Microscope examination was used to characterize phytoplankton species, and the identification was verified by a professional. Ten phytoplankton species were identified, with Haematococcus sp. selected for wastewater bioremediation due to its optimal growth under specific conditions (81 µmol m-2 s-1, pH 9, 25°C ± 2°C). Experiments showed that Haematococcus sp. significantly reduced nitrogen and phosphorus in wastewater over 7-14 days, with Treatment 3 (1000 mg/l) achieving the highest growth, chlorophyll, and nitrogen reduction, while Treatment 2 (750 mg/l) excelled in phosphorus removal. These results underscore phytoplankton's potential for sustainable, large-scale wastewater bioremediation.

苏米林坝浮游植物培养及其对水产养殖废水的生物修复能力研究。
位于北伊洛科斯省萨拉特的苏米林大坝是多种浮游植物的家园。本研究旨在分离和鉴定浮游植物种类,优化一种浮游植物在不同光照、pH和温度条件下的培养,并评估其对水产养殖废水的生物修复能力。在现场设置了三个站点,每个站点有三个采样点,间隔3-5米。从每个点收集300毫升水,使用标准程序,并运送到实验室进行浮游植物鉴定。样品在藻类培养液中孵育14天,镀条隔离,光照培养。利用显微镜对浮游植物种类进行了鉴定,鉴定由专业人员进行了验证。共鉴定了10种浮游植物,其中红球菌(Haematococcus sp.)在特定条件(81µmol m-2s-1, pH 9, 25±2°C)下生长最佳,因此被选中用于废水的生物修复。实验表明,在7 ~ 14天内,红球菌对废水中的氮、磷具有显著的还原作用,其中处理3 (1000 mg/L)的生长、叶绿素和氮还原效果最高,处理2 (750 mg/L)的除磷效果最好。这些结果强调了浮游植物在可持续的大规模废水生物修复方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Letters in Applied Microbiology
Letters in Applied Microbiology 工程技术-生物工程与应用微生物
CiteScore
4.40
自引率
4.20%
发文量
225
审稿时长
3.3 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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