Letters in Applied Microbiology最新文献_第7页

Biotransformation of naproxen by an atoxigenic Bacillus cereus group strain closely related to Bacillus anthracis: linking genomic features to biodegradative activity. 与炭疽芽孢杆菌密切相关的嗜氧蜡样芽孢杆菌群菌株对萘普生的生物转化：将基因组特征与生物降解活性联系起来。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag028

Jessica L Ortega-Balleza, Mailyn S Porras-García, Ana Verónica Martinez-Vázquez, Eyra Ortiz-Pérez, Alma D Paz-González, Gildardo Rivera

{"title":"Biotransformation of naproxen by an atoxigenic Bacillus cereus group strain closely related to Bacillus anthracis: linking genomic features to biodegradative activity.","authors":"Jessica L Ortega-Balleza, Mailyn S Porras-García, Ana Verónica Martinez-Vázquez, Eyra Ortiz-Pérez, Alma D Paz-González, Gildardo Rivera","doi":"10.1093/lambio/ovag028","DOIUrl":"10.1093/lambio/ovag028","url":null,"abstract":"Nonsteroidal anti-inflammatory drugs (NSAIDs), such as naproxen (Nap), are among the most widely consumed medications worldwide. However, wastewater treatments are inefficient at removing them, leading to their persistence in the environment. In this study, bacteria isolated from the contaminated lagoon \"La Escondida\", Reynosa, Mexico, were initially screened for tolerance to pharmaceutical drugs, including Nap. Ten bacterial isolates showed growth in the presence of both Amp (ampicillin) and Nap (100 mg l-1). Isolate P43-LBF was identified as a Bacillus group, showing high genomic similarity (ANI 98.26 %) to Bacillus anthracis but lacking the pOX1 and pOX2 virulence plasmids. It caused a degradation of 67.43 % and 43.78 % (5 and 25 mg l-1, respectively) of Nap in glucose-supplemented minimal medium in seven days. Degradation products, such as 6-O-desmethylnaproxen, were detected by Ultra Performance Liquid Chromatography Mass Spectrometry (UPLC-MS), indicating active biotransformation. Genomic analysis revealed genes associated with xenobiotic degradation, including catE, encoding catechol-2,3-dioxygenase. Additionally, genes associated with virulence and resistance were also identified, which support stress tolerance. Phylogenetic analysis of Bacillus species known for their ability to degrade aromatic compounds supports their potential application in pharmaceutical bioremediation. These findings highlight strain P43-LBF as a promising candidate for pharmaceutical bioremediation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147290113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of a blend of four Alkalihalobacillus clausii strains (O/C, N/R, SIN, and T) on gut barrier integrity and immune modulation with PPIs: an in vitro SHIME study. 四种克劳氏碱杆菌（O/C、N/R、SIN和T）混合对肠道屏障完整性和PPIs免疫调节的影响：一项体外SHIME研究

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag025

Lynn Verstrepen, Cindy Duysburgh, Mattia Van den Broeck, Marcos Iii Perez, Denis Guyonnet

{"title":"Impact of a blend of four Alkalihalobacillus clausii strains (O/C, N/R, SIN, and T) on gut barrier integrity and immune modulation with PPIs: an in vitro SHIME study.","authors":"Lynn Verstrepen, Cindy Duysburgh, Mattia Van den Broeck, Marcos Iii Perez, Denis Guyonnet","doi":"10.1093/lambio/ovag025","DOIUrl":"10.1093/lambio/ovag025","url":null,"abstract":"Alkalihalobacillus clausii is effective in alleviating PPI-induced dysbiosis; however, its effects on gut barrier function and inflammation remain unclear. This study explored A. clausii (O/C, N/R, SIN, and T) effects on gut barrier function during PPI-induced dysbiosis using Triple-M-SHIME® model. Reactors were supplemented with PPI alone (PPI control); simultaneous PPI + A. clausii (preventive); and PPI for 2 weeks followed by A. clausii (curative). Caco-2/THP1-Blue™ co-cultures were exposed to colonic SHIME® suspensions for 24 h for TEER measurement, followed by lipopolysaccharide stimulation for measurement of cytokine secretion and NF-κB. Preventive treatment did not improve inflammation-induced barrier disruption, but was slightly improved with curative treatment, particularly in the distal colon. Colonic ferments following A. clausii supplementation increased IL-10 secretion and pro-inflammatory signals in the ileum. In the proximal and distal colon, PPI treatment reduced pro-inflammatory markers during and after treatment, while increasing anti-inflammatory cytokines following prolonged incubation. Preventive treatment with A. clausii, reduced pro-inflammatory markers and IL-10, while increasing IL-6 secretion. Curative A. clausii treatment lowered pro-inflammatory markers. Enhanced colonic fermentation by A. clausii thus showed potential in improving gut barrier function and modulating inflammation with complex colon region-specific responses, particularly with curative treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic characterization of Bacillus subtilis Strain BS-Q and its cell-free-supernatant-mediated inhibition of Clostridium perfringens. 枯草芽孢杆菌菌株BS-Q的益生菌特性及其对产气荚膜梭菌的无上清抑制作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag008

Qianqian Dai, Junli Ni, Junying Lu, Weiwei Shi, Xiangjie Chen, Yibin Zhu, Yongle Song, Haiming Cai, Xuhui Lin, Minna Lv, Juan Li, Shenquan Liao, Mingfei Sun, Jianfei Zhang, Xiaohui Zhang, Qianqian Hu, Nanshan Qi

{"title":"Probiotic characterization of Bacillus subtilis Strain BS-Q and its cell-free-supernatant-mediated inhibition of Clostridium perfringens.","authors":"Qianqian Dai, Junli Ni, Junying Lu, Weiwei Shi, Xiangjie Chen, Yibin Zhu, Yongle Song, Haiming Cai, Xuhui Lin, Minna Lv, Juan Li, Shenquan Liao, Mingfei Sun, Jianfei Zhang, Xiaohui Zhang, Qianqian Hu, Nanshan Qi","doi":"10.1093/lambio/ovag008","DOIUrl":"10.1093/lambio/ovag008","url":null,"abstract":"This study characterized Bacillus subtilis (B. subtilis) BS-Q as a novel probiotic candidate against Clostridium perfringens (C. perfringens). Polyphasic analysis, including 16S rRNA gene sequencing (>99% similarity to reference B. subtilis strains), identified the isolate as BS-Q. It exhibited robust growth, and sporulation was observed. Its cell-free supernatant (CFS) demonstrated potent, broad-spectrum inhibition against C. perfringens types A, C, and G with a minimum inhibitory concentration (MIC) of 33.06 ± 0.53 μg/mL. The antibacterial activity was localized to the CFS and showed remarkable stability after exposure to harsh conditions: high temperature (100°C for 30 min), a broad pH range (3.0-11.0), and high salinity (up to 10% NaCl). Protease treatment markedly reduced the activity, confirming the proteinaceous nature of the antimicrobial compounds, which was supported by the presence of unique protein bands (25-35 kDa) in the CFS as revealed by SDS-PAGE. In conclusion, B. subtilis BS-Q produces highly stable, proteinaceous antimicrobials effective against C. perfringens. Its strong anti-clostridial activity and resilience position it as a promising probiotic and a potential antibiotic alternative for controlling necrotic enteritis in poultry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Native bradyrhizobia for soybean: genetic and functional diversity in Heihe soils, a major production zone of Heilongjiang, China. 大豆原生缓生根瘤菌：黑龙江省主要产区黑河土壤的遗传和功能多样性

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag026

Ke Li, Keyu Chen, Haoran Hao, Kaiwen Zhang, Brigitte Brunel, Wenshan Zhou, Junjie Zhang

{"title":"Native bradyrhizobia for soybean: genetic and functional diversity in Heihe soils, a major production zone of Heilongjiang, China.","authors":"Ke Li, Keyu Chen, Haoran Hao, Kaiwen Zhang, Brigitte Brunel, Wenshan Zhou, Junjie Zhang","doi":"10.1093/lambio/ovag026","DOIUrl":"10.1093/lambio/ovag026","url":null,"abstract":"Soybean (Glycine max) forms symbiotic nitrogen fixation with rhizobia, and compatible, efficient rhizobia in soils are vital for its sustainable production. This study analyzed the distribution and traits of native soybean-associated rhizobia in soils from Heihe, Heilongjiang Province, to identify strains with high symbiotic nitrogen fixation efficiency and environmental adaptability, and select candidates for local microbial inoculants to boost sustainable soybean production. Seventy-four rhizobial isolates were obtained from three Heilongjiang sampling sites using a local soybean variety, and characterized genetically and symbiotically. PCR-RFLP of IGS DNA grouped them into 9 genotypes. Multilocus sequence analysis (16S rRNA, recA, atpD, gyrB genes) placed representative strains into three known species (Bradyrhizobium japonicum, B. diazoefficiens, B. ottawaense) and an uncharacterized Bradyrhizobium group (64%, dominant). Phylogeny of nodC/nifH markers showed affiliation with symbiovar glycinearum. All strains nodulated soybean with symbiotic efficiency (67-88%); about half enhanced plant biomass. Three strains (DG28, GCZ12, SH16) showing superior symbiotic efficiency. Representative strains had varied tolerance to alkalinity, high temperature, and PEG-induced drought. Strain SH16 combined high efficiency and stress tolerance. These results provide promising candidates for local soybean inoculant development.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of rifaximin and rifampicin resistance across Clostridioides difficile ribotypes: a systematic review and meta-analysis. 艰难梭菌核型对利福昔明和利福平耐药性的评估：一项系统综述和荟萃分析。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag018

Nasser Alkhushaym, Rawan Alabbad, Yousef Saeed Alqarni, Naif Almalki, Reem Khader Alanazi, Abdullah Alhifany

{"title":"Assessment of rifaximin and rifampicin resistance across Clostridioides difficile ribotypes: a systematic review and meta-analysis.","authors":"Nasser Alkhushaym, Rawan Alabbad, Yousef Saeed Alqarni, Naif Almalki, Reem Khader Alanazi, Abdullah Alhifany","doi":"10.1093/lambio/ovag018","DOIUrl":"10.1093/lambio/ovag018","url":null,"abstract":"Clostridioides difficile is an opportunistic pathogen of the gastrointestinal tract that can cause illnesses ranging from diarrhea to pseudomembranous colitis. Recurrent Clostridioides difficile infection remains a major clinical challenge, with substantial relapse rates after standard antibiotic therapy. Emerging evidence suggests that rifaximin can be used after the conventional therapy to reduce risk of the recurrence. However, rifaximin resistance in Clostridioides difficile remains the primary concern. The aim of this systematic review and meta-analysis is to estimate the risk of rifaximin resistance in Clostridioides difficile infection and among different ribotypes. The search included PubMed, Scopus, Web of Science, and Cochrane Library for studies reporting rifaximin resistance in Clostridioides difficile isolates. After systematically screening 731 records from all databases and excluding 664 studies, a total of 67 studies were included in the meta-analysis. The findings of the meta-analaysis indicated a resistance rate of 15.1% (95% CI, 12.0%-18.9%) for rifaximin and/or rifampicin and 12.8% (95% CI, 8.8%-18.2%) for rifaximin alone. Ribotype-specific analysis revealed high rifaximin resistance in RT017 (72.3%), RT027 (47.0%), and RT018 (20.9%), while RT012, RT002, RT112, and RT014/020 demonstrated low resistance. The study finding indicate that rifaximin/rifamycin resistance in Clostridioides difficile is concerning and not randomly distributed but is more frequently associated with certain ribotypes.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Staphylococcus aureus extracellular vesicles: navigating challenges, embracing prospects. 金黄色葡萄球菌细胞外囊泡研究进展：挑战与展望。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag009

Chunyan Fu, Zeqi Li, Yanwen Sun, Shaohua Lin, Xuejing Yang, Xiaojuan Li

{"title":"Advances in Staphylococcus aureus extracellular vesicles: navigating challenges, embracing prospects.","authors":"Chunyan Fu, Zeqi Li, Yanwen Sun, Shaohua Lin, Xuejing Yang, Xiaojuan Li","doi":"10.1093/lambio/ovag009","DOIUrl":"10.1093/lambio/ovag009","url":null,"abstract":"Staphylococcus aureus has emerged as a globally widespread pathogen, and its growing antibiotic resistance poses severe challenges to human health. With the advancing research on bacterial extracellular vesicles (bEVs), S. aureus EVs, have been found to act as pathogenic factors, exacerbating the pathophysiology of S. aureus infections. This review summarizes current research on S. aureus EVs, outlining their roles in promoting virulence and contributing to antibiotic resistance, which provides insights into the pathogenic mechanisms and the emergence of resistance in S. aureus. We also summarize certain natural compounds that exhibit disruptive effects on S. aureus EVs, which may offer new strategies for treating S. aureus infections. Although S. aureus EVs aggravate disease progression, their unique properties can be harnessed through bioengineering techniques. This review further highlights the potential applications of S. aureus EVs in vaccine development, drug delivery, and innovative anti-tumor or antiviral therapies. However, the rapid emergence of new technologies and the discovery of non-vesicular nanoparticles have cast doubt on previously established findings. Therefore, this review also addresses the following questions: How should we interpret these existing discoveries? And how should we advance future research related to S. aureus EVs?","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of methicillin-resistant Staphylococcus aureus and multidrug resistance among food handlers in a hospital food and nutrition unit in Rio de Janeiro, Brazil. 巴西里约热内卢一家医院食品和营养部门食品处理人员中耐甲氧西林金黄色葡萄球菌和多药耐药性的流行情况

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag021

Rosevane de Oliveira Cunha, Douglas Guedes, Elaine de Oliveira Pinto, Simone Alves, Aline Dos Santos Garcia-Gomes

{"title":"Prevalence of methicillin-resistant Staphylococcus aureus and multidrug resistance among food handlers in a hospital food and nutrition unit in Rio de Janeiro, Brazil.","authors":"Rosevane de Oliveira Cunha, Douglas Guedes, Elaine de Oliveira Pinto, Simone Alves, Aline Dos Santos Garcia-Gomes","doi":"10.1093/lambio/ovag021","DOIUrl":"10.1093/lambio/ovag021","url":null,"abstract":"Foodborne diseases are a major public health concern, and food handlers play a key role in the transmission of pathogenic and antimicrobial-resistant microorganisms. This study evaluated the occurrence and antimicrobial resistance profiles of Staphylococcus aureus among 50 food handlers working in the food and nutrition unit of a public hospital in Rio de Janeiro, Brazil. Samples from hands and nasal cavities were analyzed using culture-based methods and identified by MALDI-TOF mass spectrometry. Staphylococcus aureus was detected in 12% of hand samples and 22% of nasal swabs. High resistance rates were observed for penicillin, erythromycin, and ampicillin. Methicillin-resistant S. aureus represented 29% of hand isolates and 47% of nasal isolates, and 26% were multidrug resistant. These findings indicate that food handlers may act as reservoirs and transmission sources of antimicrobial resistance in hospital food service settings, highlighting the need for continuous monitoring, strict hygiene practices, and antimicrobial stewardship programs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into membrane disruption and oxidative stress in Gram-positive and Gram-negative bacteria: role of sodium benzoate and phenoxyethanol. 对革兰氏阳性和革兰氏阴性细菌的膜破坏和氧化应激的见解：苯甲酸钠和苯氧乙醇的作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag022

Ishani Sharma, S M Rose, Madhu Lata, Somnath Das, Nagaraja Is Acharya, Maheshwara Naik, Samiran Mahapatra, Sharmistha Sinha

{"title":"Insights into membrane disruption and oxidative stress in Gram-positive and Gram-negative bacteria: role of sodium benzoate and phenoxyethanol.","authors":"Ishani Sharma, S M Rose, Madhu Lata, Somnath Das, Nagaraja Is Acharya, Maheshwara Naik, Samiran Mahapatra, Sharmistha Sinha","doi":"10.1093/lambio/ovag022","DOIUrl":"10.1093/lambio/ovag022","url":null,"abstract":"This study investigates the antibacterial effects of two widely used preservatives in foods, pharmaceuticals, and personal care products, sodium benzoate and phenoxyethanol, on Gram-positive bacteria Staphylococcus aureus (ATCC 6538) and Gram-negative bacteria Pseudomonas aeruginosa (MTCC 1688). Despite their widespread usage, the exact antibacterial mechanisms of these preservatives are not yet fully understood. Our study demonstrated that the presence of SB and POE lead to extensive cellular rupture, membrane damage, and intracellular content leakage in bacteria. The results were validated through biochemical assays and visualized by transmission electron microscopy. The key findings from this study reveals that P. aeruginosa when treated with SB leads to cell shrinkage, whereas POE treatment leads to cellular bulging, under varied concentrations. Further analysis demonstrates that both compounds induce intracellular aldehyde accumulation leading to an increase in oxidative stress. This increase in oxidative stress can cause protein cross-linking, DNA and membrane destabilization, leading to cellular disruption. In summary, this study sheds light on the mechanisms behind the antibacterial effects and preservative's efficacy of these well-established compounds, offering valuable information for their ongoing use and potential enhancement in microbial control strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First evidence of SO2-releasing bags controlling fungal growth, aflatoxins, and cyclopiazonic acid contamination in unshelled peanuts. 释放二氧化硫袋控制去壳花生真菌生长、黄曲霉毒素和环吡唑酸污染的首个证据。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag029

Carla Cervini, Vaibhav Verma, Carol Verheecke-Vaessen, Angel Medina, Naresh Magan

{"title":"First evidence of SO2-releasing bags controlling fungal growth, aflatoxins, and cyclopiazonic acid contamination in unshelled peanuts.","authors":"Carla Cervini, Vaibhav Verma, Carol Verheecke-Vaessen, Angel Medina, Naresh Magan","doi":"10.1093/lambio/ovag029","DOIUrl":"10.1093/lambio/ovag029","url":null,"abstract":"Peanuts (also known as groundnuts; Arachis hypogaea L.) are a globally significant cash crop but are highly susceptible to fungal contamination, particularly by Aspergillus section Flavi, which can contaminate the product with mycotoxins, including aflatoxins. This contamination poses serious food safety concerns, especially in low- and middle-income countries, limiting access to international markets. This study assessed the efficacy of slow-release SO2-bags compared to plastic bags (control) in reducing fungal growth and mycotoxin contamination in stored unshelled peanuts. Naturally contaminated and Aspergillus flavus-inoculated peanuts were stored at water activity (aw) levels of 0.85 and 0.95 for 15 days at 25°C. Fungal populations were monitored, and aflatoxins and cyclopiazonic acid were analysed using LC-MS/MS qTRAP. SO2-releasing bags completely inhibited fungal growth in naturally contaminated peanuts and significantly reduced A. flavus population with effectiveness dependent on water activity. Additionally, SO2-releasing bags suppressed mycotoxin production. This study demonstrates, for the first time, the effectiveness of SO2-releasing bags in preventing fungal spoilage and mycotoxin contamination in postharvest peanuts, offering a promising solution for enhancing peanut safety and quality.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities. 使用元条形码检测在基于培养的海洋沉积物细菌群落分析中快速开始的培养偏差。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag020

Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park

{"title":"Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities.","authors":"Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park","doi":"10.1093/lambio/ovag020","DOIUrl":"10.1093/lambio/ovag020","url":null,"abstract":"When cultivation-based microbiology is used to isolate strains from environmental samples, the cultured populations may not represent ecologically relevant taxa in the source community. To address this, we employed pre-cultivation metabarcoding to establish a baseline community profile and detect cultivation bias. Using time-resolved cultivation of marine sediment bacteria, we demonstrated the need for initial community characterization. Sediment-derived microbiomes were cultured in Marine Broth 2216 and analyzed using 16S rRNA gene metabarcoding at 0, 6, 12, 18, and 24 h. A rapid 10-fold reduction in alpha diversity was observed within the 6 h (from 1029 amplicon sequence variants to 34-106), with the genus Vibrio reaching near-complete dominance (>95%) from 18 to 24 h, while environmentally dominant taxa such as Acinetobacter were quickly excluded. This dramatic shift illustrates that, without baseline characterization, cultivation-induced artifacts cannot be clearly distinguished from ecologically meaningful patterns. Fast-growing generalists can quickly outcompete ecologically significant taxa, distorting isolation outcomes and hindering the recovery of functionally important microorganisms. We show that metabarcoding at 0 h can identify cultivation biases, help interpret isolation results, and suggest targeted strategies for recovering ecologically relevant taxa. This integrated approach facilitates more accurate recovery and analysis of functionally significant microbial diversity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0