Letters in Applied Microbiology最新文献_第7页

Combination therapy strategies against multidrug resistant bacteria in vitro and in vivo. 体外和体内多重耐药细菌的联合治疗策略。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae129

Daliang Wang, Jie Yang, Lilan Yang, Yanglin Du, Qunchao Zhu, Chendong Ma, Dongdong Zhou

{"title":"Combination therapy strategies against multidrug resistant bacteria in vitro and in vivo.","authors":"Daliang Wang, Jie Yang, Lilan Yang, Yanglin Du, Qunchao Zhu, Chendong Ma, Dongdong Zhou","doi":"10.1093/lambio/ovae129","DOIUrl":"10.1093/lambio/ovae129","url":null,"abstract":"Exploring effective combination antibacterial therapies has become a research focus. This study selected seven common antibiotics to perform a series of tests on different Gram-negative bacteria isolated from clinical samples of chronic obstructive pulmonary disease patients. More than 70% of the strains exhibited multidrug resistance but remained sensitive to polymyxin B. The checkerboard assay revealed a significant synergistic effect between polymyxin B and tetracycline against different resistant strains, with fractional inhibitory concentration index values consistently below 0.5. Further time-kill curve analysis demonstrated that the use of minimal inhibit concentration of polymyxin B or tetracycline alone had limited bactericidal effects, while their combination significantly reduced bacterial counts by 2-3 log colony-forming units within 12 h. Additionally, the survival rate of larvae treated with the polymyxin B and tetracycline combination was significantly higher than that of the mono-therapy and untreated groups. In brief, this study demonstrates that the combination of polymyxin B and tetracycline exhibits potent antibacterial activity against multidrug resistant Gram-negative bacteria both in vitro and in vivo.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivar-dependent differences in plant bud microbiome and functional gene pathways in woody plants commonly used in urban green space. 城市绿地常用木本植物植物芽微生物组和功能基因通路的栽培品种依赖性差异。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae110

Marja I Roslund, Polina Galitskaya, Mika Saarenpää, Aki Sinkkonen

{"title":"Cultivar-dependent differences in plant bud microbiome and functional gene pathways in woody plants commonly used in urban green space.","authors":"Marja I Roslund, Polina Galitskaya, Mika Saarenpää, Aki Sinkkonen","doi":"10.1093/lambio/ovae110","DOIUrl":"10.1093/lambio/ovae110","url":null,"abstract":"Plant richness and microbiota have been associated with plant health; hardly any studies have investigated how plant taxa differs in microbiota in the context of human health. We investigated the microbial differences in buds of 83 woody plant taxa used in urban green spaces in hemiboreal climate, using 16S rRNA and whole metagenome shotgun sequencing. Bud microbial community was the richest in Cotoneaster Nanshan and C. integerrimus, and Malus domestica cultivars \"Sandra\" and \"Lobo\" and poorest in Ribes glandulosum. Metagenomic shotgun sequencing of two M. domestica and four Ribes varieties confirmed differences in taxa in bud microbiota and indicated higher siderophore synthesis in Malus. Microbial richness, including bacteria, archaea, and viruses, and functional richness of gene pathways was higher in Malus compared to Ribes. The 10 most abundant amplicon sequence units, often referred as species, belonged to the phylum Proteobacteria. The differences between plant taxa were evident in classes Alpha- and Gammaproteobacteria, known for potential human health benefits. Since environmental microbiota contributes to human microbiota and immunoregulation, horticultural cultivars hosting rich microbiota may have human health benefits. Further studies are needed to confirm the effectiveness of microbially-oriented plant selection in optimizing human microbiota and planetary health.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of heterotrophic endophytic bacteria in four kinds of edible raw vegetables: species distribution, antibiotic resistance, and related genes. 四种可食生蔬菜中异养内生细菌的种类分布、耐药性及相关基因

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae120

Chong-Miao Zhang, Qiao-Qiao Yuan, Yong-Qiang Li, An Liu

{"title":"Characteristics of heterotrophic endophytic bacteria in four kinds of edible raw vegetables: species distribution, antibiotic resistance, and related genes.","authors":"Chong-Miao Zhang, Qiao-Qiao Yuan, Yong-Qiang Li, An Liu","doi":"10.1093/lambio/ovae120","DOIUrl":"10.1093/lambio/ovae120","url":null,"abstract":"This study aimed to explore antibiotic resistance characteristics and species of heterotrophic endophytic bacteria (HEB) in four kinds of edible raw vegetables, including radishes, lettuces, onions, and tomatoes. A total of 144 HEB were isolated and tested for resistance to sulfamethoxazole (SMZ), tetracycline (TET), cefotaxime (CTX), and ciprofloxacin (CIP), and their species were identified by 16S rRNA gene sequencing. Antibiotic resistance genes (ARGs) and class I integron in antibiotic-resistant isolates were analyzed by polymerase chain reaction. The results showed radishes had the highest, while tomatoes had the lowest concentration of antibiotic-resistant HEB. SMZ and CTX were predominant antibiotic-resistant phenotypes in HEB. The multi-resistant phenotypes, the combinations SMZ-TET-CTX and SMZ-TET-CIP, accounted for 9.34% of all antibiotic-resistant phenotypes, mainly in radishes and lettuces. Bacillus, Pseudomonas, Staphylococcus, and Stenotrophomonas showed resistance to two antibiotics and existed in more than one kind of vegetable, and were the main carriers of sul1, sul2, blaTEM, and intI1 genes. Therefore, these four genera were considered potential hosts of ARGs in edible raw vegetables. The study provides an early warning regarding health risks associated with ingesting antibiotic-resistant bacteria through raw vegetable consumption.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of physiological functions and metabolome of Vibrio alginolyticus by quorum-regulatory sRNA, Qrr1. 群体调节sRNA （Qrr1）对溶藻弧菌生理功能和代谢组的调节

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae126

Yanni Zhao, Ruobing Wu, Xuan Wu, Ningning Zhou, Jiamin Ren, Wang Liu, Rui Yu, Senhu Zhang, Jinfang Yang, Hua Li, Huan Liu

{"title":"Modulation of physiological functions and metabolome of Vibrio alginolyticus by quorum-regulatory sRNA, Qrr1.","authors":"Yanni Zhao, Ruobing Wu, Xuan Wu, Ningning Zhou, Jiamin Ren, Wang Liu, Rui Yu, Senhu Zhang, Jinfang Yang, Hua Li, Huan Liu","doi":"10.1093/lambio/ovae126","DOIUrl":"10.1093/lambio/ovae126","url":null,"abstract":"Vibrio alginolyticus, the causative agent of aquatic vertebrates and invertebrates, can cause severe infections (e.g. septicemia, gill necrosis, and surface ulcers) and high mortality in aquatic organisms, leading to serious economic losses in global aquaculture. Small non-coding RNAs (sRNAs), emerging modulators of gene expression, played vital regulatory roles in virulence, pathogenicity, and physiological metabolism of bacteria. In this work, the modulation of physiological functions and metabolome of V. alginolyticus by the quorum-regulatory sRNA, Qrr1, was figured out. We found that the deletion of qrr1 induced significant cell shape elongation. Meanwhile, Qrr1 could inhibit the production of alkaline serine protease by weakening the expression of main regulator LuxR in the quorum sensing (QS) system. Moreover, the untargeted metabolomics and lipidomics approaches showed that most of nucleotides, organic acids, carbohydrates, and lipidome (both lipid content and category) were significantly altered in response to the qrr1 deletion. Spearman correlation analysis demonstrated that most of the intermediates involved in glutamate metabolism, sphingolipid metabolism, and glycerolipid metabolism displayed high correlations with cell virulence factors. These findings illuminate the mechanism of bacterial virulence regulation and further exploit potential therapeutic targets for virulence prevention in V. alginolyticus.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of polyaromatic hydrocarbon polluted sewage sludge soil employing a bacterial consortium and phytotoxicity evaluation. 利用细菌联合体对受多芳烃污染的污水污泥土壤进行生物修复和植物毒性评估。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae130

Gulfishan Khan, Anshul Tiwari, Devendra K Patel, Sadasivam Anbumani, Natesan Manickam

{"title":"Bioremediation of polyaromatic hydrocarbon polluted sewage sludge soil employing a bacterial consortium and phytotoxicity evaluation.","authors":"Gulfishan Khan, Anshul Tiwari, Devendra K Patel, Sadasivam Anbumani, Natesan Manickam","doi":"10.1093/lambio/ovae130","DOIUrl":"10.1093/lambio/ovae130","url":null,"abstract":"A consortium of five distinct bacterial strains was evaluated for their ability to biodegrade multiple polyaromatic hydrocarbons (PAHs) in sewage sludge under microcosm studies. The presence of PAHs was determined from the sludge samples collected during pre- and post-monsoon seasons from three different wastewater treatment plants (WWTPs). Among the 16 PAHs found, the lowest concentration detected was 1.75 ng g-1 of benz(k)fluoranthene, and the highest concentration of 5.41 mg g-1 indeno(1,2,3-cd) pyrene was found in both dry and wet samples, perhaps owing to its multiple origin of contamination. A bacterial consortium comprising of well characterized bacteria Stenotrophomonas maltophilia IITR87, Ochrobactrum anthropi IITR07, Microbacterium esteraromaticum IITR47, Pseudomonas aeruginosa IITR48, and Pseudomonas mendocina IITR46 employed for PAHs bioremediation in a microcosm study. In 20 days, 65%-70% of PAHs were remediated, and low molecular weight PAHs such as naphthalene, phenanthrene, and pyrene showed enhanced degradation. Bioremediated samples showed a significant reduction in phytotoxicity using plant germination of wheat (Triticum aestivum), black chickpea (Cicer arietinum), and mustard (Brassica juncea), whereas the contaminated soil showed severe inhibition of plant growth. The results comprehensively suggest a possible remediation option for PAHs occurring in complex sewage sludge, preventing further contamination into other environmental compartments.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Conserved CRISPR arrays in Salmonella enterica serovar Infantis can serve as qPCR targets to detect Infantis in mixed serovar populations. 修正：肠炎沙门氏菌血清型婴儿中保守的CRISPR序列可作为混合血清型人群中检测婴儿的qPCR靶点。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae131

引用次数: 0

Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B. 重组酯酶 B 对耐亚胺培南铜绿假单胞菌的再敏感性和肠杆菌科细菌对头孢菌素的敏感性的恢复。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae118

Wirongrong Whangsuk, Punyawee Dulyayangkul, Suvit Loprasert, James M Dubbs, Paiboon Vattanaviboon, Skorn Mongkolsuk

{"title":"Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B.","authors":"Wirongrong Whangsuk, Punyawee Dulyayangkul, Suvit Loprasert, James M Dubbs, Paiboon Vattanaviboon, Skorn Mongkolsuk","doi":"10.1093/lambio/ovae118","DOIUrl":"10.1093/lambio/ovae118","url":null,"abstract":"Sphingobium sp. SM42 Esterase B (EstB) is an enzyme with a dual function in degrading dibutyl phthalate and catalyzing the cleavage of the C-S bond in C3-sidechains of the dihydrothiazine ring of cephalosporins, generating more active β-lactam derivatives. Global prokaryotic genome analysis revealed the existence of a gene identical to estB in Pseudomonas aeruginosa strain PS1 suggesting a horizontal gene transfer event involving estB. To investigate the effect of ectopic expression of EstB in the periplasm of P. aeruginosa and several Enterobacteriaceae on antibiotic susceptibility levels, plasmid, pEstB, carrying a recombinant EstB fused with the signal peptide from Escherichia coli outer membrane protein A (OmpA) for periplasmic localization was constructed. The expression of EstB in the periplasm of P. aeruginosa and the Enterobacteriaceae: E. coli, Klebsiella pneumoniae, and Salmonella enterica serovar Typhi, increased susceptibility to carbapenems and cephalosporins. EstB reversed the imipenem resistance of P. aeruginosa ΔmexS and restored the changes in susceptibility to cephalosporins conferred by the downregulation of the outer membrane proteins, OmpK35 and OmpK36, in K. pneumoniae ΔramR-ompK36 to wild-type level. The introduction of EstB to the periplasmic space of Gram-negative bacteria can increase carbapenem and cephalosporin susceptibility.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depth-dependent bacterial colonization on model chitin particles in the open ocean. 开阔海洋中甲壳素模型颗粒上随深度变化的细菌定殖。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae107

Cordelia Roberts, Kimberley Bird, Nathan Chrismas, Susan Hartman, Michael Cunliffe

{"title":"Depth-dependent bacterial colonization on model chitin particles in the open ocean.","authors":"Cordelia Roberts, Kimberley Bird, Nathan Chrismas, Susan Hartman, Michael Cunliffe","doi":"10.1093/lambio/ovae107","DOIUrl":"10.1093/lambio/ovae107","url":null,"abstract":"Sinking particles transport carbon from the surface to the deep ocean. Microbial colonization and remineralization are important ecosystem services constraining ocean biogeochemistry by recycling and redistributing nutrients from the surface to the deep ocean. Fragmentation of particles by zooplankton and the resulting colonization by microorganisms before ingestion, known as 'microbial gardening', allows for trophic upgrading and increased microbial biomass for detritivorous zooplankton. Using model chitin particles incubated with seawater collected from the surface, mesopelagic and bathypelagic depths in the Northeast Atlantic Ocean, we determined particle-attaching bacterial communities to identify general and depth-specific candidates of particle colonization. Comparison of particle-attached communities at the amplicon sequence variant level showed that bacteria found on surface particles were also colonizers in the bathypelagic, in line with sinking particles promoting vertical connectivity. Bathypelagic particle-attached communities were most diverse. We propose that some particle colonizers attach to the surface and sink out with the particle, whilst other colonizers are depth-specific. This suggests that candidates for particle colonization differ with depth, which may be important when considering the implications for the delivery of ecosystem services, including carbon cycling and the role they play for zooplankton grazers.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter. 新型光谱学方法揭示大肠杆菌发酵罐最佳培养条件。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae123

Jia Chen, Huakun Wu, Jingru Chen, Jia Zheng, Wen Liu, Meifang Yu

{"title":"Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter.","authors":"Jia Chen, Huakun Wu, Jingru Chen, Jia Zheng, Wen Liu, Meifang Yu","doi":"10.1093/lambio/ovae123","DOIUrl":"https://doi.org/10.1093/lambio/ovae123","url":null,"abstract":"Fermentation engineering is critical for mass-producing chemicals, food additives, and medicines, where optimal culture conditions maximize microbial growth and metabolite production. Although monitoring bacterial growth during fermentation is critical, there is a lack of a non-invasive and sensitive method to directly monitor the bacterial metabolism. In this paper, a novel optical monitoring method is proposed based on tunable diode laser absorption spectroscopy. First, the detecting system consisting of a laser, detection, a homemade board, and an incubator is established and verified to be able to monitor the metabolite production of CO2 in Escherichia coli through a 25-h detection period. Second, the quantitative growth rate analysis method is specified by calculating the threshold time (TT) intervals between consecutive dilution gradients, and the threshold with the least sum of residuals is chosen as the optimal threshold. Finally, alongside varied pH and temperature settings in a simulated fermenter, we elucidated the influence of these factors on E. coli metabolism curves and calculated the growth rates via TT, identifying 38°C as the optimal temperature and 7.0 as the optimal pH. This study presents a novel approach to reveal optimal culture conditions during fermentation holding promises for online real-time monitoring in the future.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"77 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and scaling up of extracellular polysaccharide production by submerged culture of Ganoderma lucidum on starch-containing medium using response surface methodology and laboratory bioreactors of various designs. 利用响应面方法学和各种设计的实验室生物反应器，优化和扩大灵芝在含淀粉培养基上的浸没培养生产胞外多糖的规模。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae115

Tatyana S Tikhomirova, Maxim R Taraskevich, Yuriy A Lepekhin, Marina P Shevelyova, Vitaliy A Nemashkalov

{"title":"Optimization and scaling up of extracellular polysaccharide production by submerged culture of Ganoderma lucidum on starch-containing medium using response surface methodology and laboratory bioreactors of various designs.","authors":"Tatyana S Tikhomirova, Maxim R Taraskevich, Yuriy A Lepekhin, Marina P Shevelyova, Vitaliy A Nemashkalov","doi":"10.1093/lambio/ovae115","DOIUrl":"10.1093/lambio/ovae115","url":null,"abstract":"Basidiomycetes, known for their production of bioactive compounds, traditionally use simple sugars for fermentation. However, their ability to degrade complex plant polysaccharides through enzyme secretion presents potential for the use of renewable raw materials. This study focused on the optimization of exopolysaccharide (EPS) production and efficient substrate consumption by Ganoderma lucidum using response surface methodology (RSM). Using an optimized medium containing 15 g⋅l-1 wheat starch, 0.375 g⋅l-1 NH4Cl, and 0.75 g⋅l-1 CaCl2 (C/N ratio of 40), a significant increase in EPS concentration from 121.1 ± 10.2 mg⋅l-1 to 229.0 ± 20.3 mg⋅l-1 and starch degradation degree (SDD) from 9.1% to 57.6% was achieved after 9 d of submerged cultivation. Scale-up experiments were conducted in both column and stirred tank bioreactors, employing submerged and immobilized cultivation modes. Submerged cultivation in the column bioreactor yielded the highest process desirability of 0.56, achieving EPS concentration of 192.5 ± 5.4 mg⋅l-1 and 60.2% SDD within 7 d. These results highlight the potential of the used column bioreactor for efficient and rapid EPS production. Notably, bioreactor experiments revealed local maxima in EPS content at specific time points, suggesting that cell wall degradation, potentially induced by shear stress, may contribute to the release of polysaccharides into the culture broth.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0