Letters in Applied Microbiology最新文献_第6页

Occurrence of multidrug-resistant ESBL-producing Enterobacteriaceae in livestock and in silico identification of probable catalytic domains in circulating ESBL variants. 畜禽中产生多重耐药ESBL的肠杆菌科细菌的出现及循环ESBL变体中可能催化结构域的计算机鉴定

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovaf003

Jai Sunder, Ayan Mukherjee, T Sujatha, Arun Kumar De, Debasis Bhattacharya, Samiran Bandyopadhyay, Aditya Paul, Indranil Samanta

{"title":"Occurrence of multidrug-resistant ESBL-producing Enterobacteriaceae in livestock and in silico identification of probable catalytic domains in circulating ESBL variants.","authors":"Jai Sunder, Ayan Mukherjee, T Sujatha, Arun Kumar De, Debasis Bhattacharya, Samiran Bandyopadhyay, Aditya Paul, Indranil Samanta","doi":"10.1093/lambio/ovaf003","DOIUrl":"10.1093/lambio/ovaf003","url":null,"abstract":"This study was conducted to detect the occurrence and phenotypic resistance pattern of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in livestock using docking-based analysis to reveal the classes of antibiotics against which ESBL-producers are active. Rectal swabs from healthy cattle (n = 100), goats (n = 88), and pigs (n = 66) were collected from backyard farms in Andaman and Nicobar Island (India). In total, 304 isolates comprising Escherichia coli (131), Salmonella (75), and Klebsiella pneumoniae (98) were recovered. The phenotypic resistance pattern showed that the highest resistance was found against erythromycin, followed by ampicillin/cloxacillin, ciprofloxacin, amoxicillin/clavulanic acid, and oxytetracycline. Screening of the isolates demonstrated maximum presence of blaTEM-Type, followed by blaCTX-M-Type, and blaSHV-Type. Sequence homology revealed the presence of CTX-M-15, SHV-228, SHV-40, and TEM-82. The possible interactions of CTX-M-15 with cephalosporins and SHV with carbapenems have been identified by docking analysis. The study revealed the occurrence of ESBL-producers with circulating emerging ESBL variants like SHV-40, along with the common variants with clinical relevance (CTX-M-15, TEM-82), highlighting the potential role of livestock as reservoirs for antimicrobial resistance genes. Identifying these ESBLs in livestock is crucial for understanding their contribution to the dissemination of antimicrobial resistant bacteria into the human food chain.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, validation, and implementation of multiplex digital PCR assays for simultaneous quantification of multiple targets. 设计，验证和实施多重数字PCR分析，同时定量多个目标。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae137

Jolinda de Korne-Elenbaas, Lea Caduff, Adrian Lison, Rachel McLeod, Melissa Pitton, Charles Gan, Timothy R Julian

{"title":"Design, validation, and implementation of multiplex digital PCR assays for simultaneous quantification of multiple targets.","authors":"Jolinda de Korne-Elenbaas, Lea Caduff, Adrian Lison, Rachel McLeod, Melissa Pitton, Charles Gan, Timothy R Julian","doi":"10.1093/lambio/ovae137","DOIUrl":"10.1093/lambio/ovae137","url":null,"abstract":"Quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) are applied for quantifying molecular targets in disease diagnostics, pathogen detection, and ecological monitoring. Uptake of dPCR is increasing due to its higher quantification accuracy relative to qPCR, which stems from its independence from standard curves and its increased resistance to PCR inhibitors. Throughput can be increased through multiplexing, which allows simultaneous quantification of multiple targets. However, multiplexing with dPCR faces unique challenges relative to qPCR. Here, we describe the three-phase development process of non-competing multiplex dPCR assays using target-specific fluorescently labeled hydrolysis probes. We highlight common challenges encountered, along with recommended solutions. Phase 1: In silico assay design; target-specific primers and probes are selected or designed, potential issues with primer and probe interactions are identified, and fluorophores and quenchers are chosen based on dPCR instrumentation. Phase 2: Wet-lab validation; assays are benchmarked using positive controls. Insufficient performance leads to assay redesign, as needed. Phase 3: Assay implementation; assay specificity and sensitivity are validated on relevant sample matrices. Finally, we provide recommendations on the future design and standardization of multiplexed dPCR assays, highlighting the need for better in silico predictions of assay performance, standardizing positive controls, and automating partition classification systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thank you to reviewers. 感谢审稿人。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovaf001

引用次数: 0

Antibacterial, antibiofilm, and gene expression assessment of ajwain (Trachyspermum ammi) essential oil on drug-resistant gastrointestinal pathogens and its combination effect with ampicillin. 阿月浑子（Trachyspermum ammi）精油对耐药性胃肠道病原体的抗菌、抗生物膜和基因表达评估及其与氨苄西林的联合作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae138

Mahdi Asghari Ozma, Niloofar Fallahi Alileh, Amin Abbasi, Sina Mahdavi, Manouchehr Fadaee, Javad Nezhadi, Masoud Asghari Ozma, Mohammad Asgharzadeh, Hossein Samadi Kafil

{"title":"Antibacterial, antibiofilm, and gene expression assessment of ajwain (Trachyspermum ammi) essential oil on drug-resistant gastrointestinal pathogens and its combination effect with ampicillin.","authors":"Mahdi Asghari Ozma, Niloofar Fallahi Alileh, Amin Abbasi, Sina Mahdavi, Manouchehr Fadaee, Javad Nezhadi, Masoud Asghari Ozma, Mohammad Asgharzadeh, Hossein Samadi Kafil","doi":"10.1093/lambio/ovae138","DOIUrl":"10.1093/lambio/ovae138","url":null,"abstract":"Essential oils are natural substances used as therapeutic agents and food preservatives to inhibit harmful microorganisms. This study aimed to assess the synergistic effect of Trachyspermum ammi essential oil and ampicillin on antibiotic-resistant gastrointestinal pathogens, including Escherichia coli, Enterococcus faecalis, Shigella flexneri, and Salmonella serotype Typhimurium. Using gas chromatography-mass spectrometry (GC-MS), the main components of T. ammi essential oil were identified as thymol, gamma terpenes, and cymene. The antibacterial and antibiofilm properties were evaluated by minimum inhibitory concentration (MIC), disk diffusion, and microtiter plate methods, revealing MIC values of 2, 1, 4, and 4 mg ml-1 for E. coli, E. faecalis, S. flexneri, and S. Typhimurium, respectively, and inhibition zones between 10 and 14 mm. Pathogens were examined for their biofilm-related virulence genes, including aggR, esp, icsA, and fliC, using real-time polymerase chain reaction (RT-PCR) in E. coli, E. faecalis, S. flexneri, and S. Typhimurium, respectively. The methyl thiazole tetrazolium (MTT) assay was used to evaluate the essential oil's effect on the viability of human embryonic kidney 293 (HEK293) cells, which showed cell viability of over 80%. The combination of T. ammi oil and ampicillin demonstrated a synergistic effect, and biofilm formation was inhibited. E. faecalis exhibited the greatest sensitivity, while S. flexneri exhibited the lowest sensitivity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of Amikacin-loaded carbopol hydrogel for topical treatment of Mycobacterium marinum skin infections. 载阿米卡霉素卡波醇水凝胶局部治疗海洋分枝杆菌皮肤感染的研制与评价。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae134

Praise Mary Francis, Vivek Vinod, V Anil Kumar, C Gopi Mohan, Raja Biswas

{"title":"Development and evaluation of Amikacin-loaded carbopol hydrogel for topical treatment of Mycobacterium marinum skin infections.","authors":"Praise Mary Francis, Vivek Vinod, V Anil Kumar, C Gopi Mohan, Raja Biswas","doi":"10.1093/lambio/ovae134","DOIUrl":"10.1093/lambio/ovae134","url":null,"abstract":"Mycobacterium marinum is a slow growing Non-Tuberculosis Mycobacteria known to cause skin and subcutaneous tissue infections known as \"fish tank granuloma\" in humans. Treatment of M. marinum skin infections can last for several months or even years. Intravenous or intramuscular administration of Amikacin, a bactericidal inhibitor, in a multidrug regimen is a good therapeutic option in treating M. marinum infections. No topical hydrogels are currently available to treat M. marinum skin infections. We developed a 2.5% amikacin-loaded carbopol hydrogel containing the permeation enhancers 1% Tween-80 and 1% PEG (AMK-CPTP) for topical application to treat M. marinum skin infections. A molecular docking study of carbopol-Amikacin interaction predicted a stable structure and was confirmed with rheological characterization. The developed gel was found to be non-hemolytic, cytocompatible, and non-irritant. Sustained release of Amikacin from the AMK-CPTP hydrogel was observed, with a release of 60% Amikacin after 48 h. The antibacterial activity and skin permeability of amikacin released from AMK-CPTP were assessed using both in vitro and ex vivo assays. Overall. AMK-CPTP demonstrated anti-microbial activity against M. marinum with a 35% increase in skin permeation on the addition of Tween-80 and PEG. AMK-CPTP could be used to treat M. marinum skin infections.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic antimicrobial effects of thiamine dilauryl sulphate combined with the heartwood extract of Pterocarpus marsupium and antifungal mechanism in their combination. 硫酸硫胺二auryl与有袋翼果心材提取物的协同抑菌作用及其协同抑菌机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae139

Moe Manbo Inoue, Hideki Kurotaki, Naoko Mizuhara, Akira Ogita, Kazuyori Matsumoto, Ken-Ichi Fujita

{"title":"Synergistic antimicrobial effects of thiamine dilauryl sulphate combined with the heartwood extract of Pterocarpus marsupium and antifungal mechanism in their combination.","authors":"Moe Manbo Inoue, Hideki Kurotaki, Naoko Mizuhara, Akira Ogita, Kazuyori Matsumoto, Ken-Ichi Fujita","doi":"10.1093/lambio/ovae139","DOIUrl":"10.1093/lambio/ovae139","url":null,"abstract":"Thiamine dilauryl sulphate (TDS) exerts a bacteriostatic effect against Gram-positive bacteria and is used as a food additive. The heartwood extract of the Indian kino tree (Pterocarpus marsupium), a medicinal tree native to India, shows weak antimicrobial effects against bacteria and superficial dermatophyte-infective fungi. Herein, we report that the combination of TDS and P. marsupium extract exerts an additive or synergistic antimicrobial effect against various food spoilage microorganisms. Among the 13 species tested, this combination showed synergistic antimicrobial effects against Bacillus subtilis, Lactococcus lactis, Lactobacillus plantarum, and Candida albicans in an agar dilution method. We also found that the combination promoted the production of reactive oxygen species against a model yeast Saccharomyces cerevisiae using a fluorescnt 2',7'-dichlorofluorescein diacetate. Furthermore, the combination restricted drug efflux from yeast cells via efflux pumps in an efflux assay using a fluorescent rhodamine 6 G. These results suggest that the antifungal effect of TDS is improved in combination with P. marsupium extract via oxidative stress induction and drug efflux restriction. The combination of TDS and P. marsupium extract has a low environmental impact and toxicity when used in foods; therefore, it is also expected to be used in food and industrial products in the future.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of rice and edible starch compound ratios, starch type, and rice nutrients on Burkholderia gladioli pathovar cocovenenans growth and bongkrekic acid production in wet rice noodles and starch products. 大米与食用淀粉复合比例、淀粉种类和大米营养成分对湿米线和淀粉制品中角兰伯克氏菌生长和产酸的影响

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae124

Jingmin Huang, Jianfei Huang, Xiaoqing Liu, Leshi Li, Zeyu Lin, Xiaole Liu, Xintian Lai, Lirong Kang, Jingru Liao, Jing Chen

{"title":"The effect of rice and edible starch compound ratios, starch type, and rice nutrients on Burkholderia gladioli pathovar cocovenenans growth and bongkrekic acid production in wet rice noodles and starch products.","authors":"Jingmin Huang, Jianfei Huang, Xiaoqing Liu, Leshi Li, Zeyu Lin, Xiaole Liu, Xintian Lai, Lirong Kang, Jingru Liao, Jing Chen","doi":"10.1093/lambio/ovae124","DOIUrl":"10.1093/lambio/ovae124","url":null,"abstract":"Bongkrekic acid (BA) toxin, produced by Burkholderia gladioli pathovar cocovenenans bacteria, has been implicated in foodborne illness outbreaks. BA poisoning is associated with rice noodle consumption; hence, this study investigated B. cocovenenans growth and BA production in wet rice noodles comprising varying starch ratios, starch types, rice nutrients, and saccharides. Noodles with 100% rice flour had BA concentrations 47.7 times higher than that of 100% starch samples (30.53 ± 1.08 mg∙kg-1 vs 0.65 ± 0.03 mg∙kg-1). Moreover, protein powder and glucose significantly promoted BA production (P < 0.05). Corn starch with 120 μl∙100 ml-1 rice oil had a BA concentration of 0.23 ± 0.01 mg∙kg-1, which was 31% lower than those without rice oil. Acid-treated starch had a BA concentration 7.24 times higher than that of other natural starch and no BA was detected in acetate starch. Of the carbohydrates tested, only hexacarbon sugars stimulated BA production. Overall, the degree of carbohydrate hydrolysis critically influenced BA formation. Increasing the use of edible starch and reducing the amount of polysaccharides hydrolyzed into monosaccharides during production will mitigate the risk of B. cocovenenans-related food poisoning in rice noodles and starch products. Rice oil serves as a possible additive to hinder BA production.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical characterization and evaluation of antiviral activity of two fucoidans extracted from Mediterranean brown seaweeds, Padina pavonica and Dictyopteris membranacea. 地中海褐海带Padina pavonica和Dictyopteris membraneides的翻译结果：

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovaf002

Safa Amri, Lamjed Bouslama, Zeineb Mzoughi, Fatma Nouira, Hatem Majdoub, Abderrahman Bouraoui

{"title":"Chemical characterization and evaluation of antiviral activity of two fucoidans extracted from Mediterranean brown seaweeds, Padina pavonica and Dictyopteris membranacea.","authors":"Safa Amri, Lamjed Bouslama, Zeineb Mzoughi, Fatma Nouira, Hatem Majdoub, Abderrahman Bouraoui","doi":"10.1093/lambio/ovaf002","DOIUrl":"10.1093/lambio/ovaf002","url":null,"abstract":"Brown seaweeds are known for their bioactive compounds, particularly sulfated polysaccharides such as fucoidans, which have demonstrated antiviral properties. However, limited studies have focused on the antiviral potential of fucoidans extracted from Mediterranean brown seaweeds. In this study, two brown seaweeds Padina pavonica and Dictyopteris membranacea (Fuc-Pad and Fuc-Dic, respectively) were collected from monastir coasts, Tunisia, and a specific extraction protocol was employed to obtain fucoidans. The main objective of this study was to evaluate their antiviral activity against Herpes simplex virus type 2 (HSV-2) and coxsackievirus B3 (CVB-3). Fuc-Pad and Fuc-Dic exhibited potent antiviral activity with high selectivity indexes (>158 780 and 3125 for Fuc-Pad and 6101 and 84 for Fuc-Dic against HSV-2 and CVB-3, respectively). On the other hand, the two brown algae demonstrated different mechanisms of antiviral action according to virus type since they inhibited HSV-2 during the adsorption and penetration stages likely through interaction with cellular receptors that block viral binding while directly inhibiting CVB-3 by blocking specific viral ligands, preventing their attachment to host cell receptors. This antiviral activity seems to be associated with the high degree of sulfating of the fucoidans. These results may suggest the possibility of developing new antiviral drugs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysine succinylome analysis of MRSA reveals critical roles in energy metabolism and virulence. MRSA的赖氨酸琥珀酰酶分析揭示了能量代谢和毒力的关键作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovaf004

Xiangqin Zhu, Hui Min, Yishan Tang, Min Gao

{"title":"Lysine succinylome analysis of MRSA reveals critical roles in energy metabolism and virulence.","authors":"Xiangqin Zhu, Hui Min, Yishan Tang, Min Gao","doi":"10.1093/lambio/ovaf004","DOIUrl":"10.1093/lambio/ovaf004","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus's (MRSA) resistance poses a global health challenge. This study investigates lysine succinylation in MRSA using proteomics and bioinformatics approaches to uncover metabolic and virulence mechanisms, with the goal of identifying novel therapeutic targets. Mass spectrometry and bioinformatics analyses mapped the MRSA succinylome, identifying 8048 succinylation sites on 1210 proteins. These analyses included Gene Ontology annotation, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, and protein-protein interaction (PPI) network construction (e.g. using the STRING database, a widely used online tool for analyzing protein-protein interactions), providing a comprehensive functional and interactive landscape of succinylated proteins. The succinylated proteins were predominantly involved in cytoplasmic metabolic processes, with enrichment in glycolysis/gluconeogenesis and the tricarboxylic acid cycle. Both of these pathways are critical for MRSA's energy production, growth, and virulence, supplying the necessary metabolic intermediates and energy to support bacterial survival and pathogenicity. Motif analysis revealed 13 conserved motifs, while PPI analysis highlighted fibronectin-binding protein A (FnbA) as a central virulence factor. Succinylation significantly influences MRSA's metabolism and virulence, potentially impacting biofilm by modifying key proteins such as FnbA, bifunctional autolysin, and S-ribosylhomocysteine lyase(LuxS). These findings provide new avenues for developing antibiofilm strategies and therapeutic interventions against MRSA.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Antimicrobial potential of Streptomyces sp. NP73 isolated from the forest soil of Northeast India against multi-drug resistant Escherichia coli. 更正：从印度东北部森林土壤中分离的Streptomyces sp. NP73对多重耐药大肠杆菌的抗菌潜力。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae132

引用次数: 0