Letters in Applied Microbiology最新文献_第6页

Characterization, antifungal activity, and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains. 菝葜精油对假丝酵母菌和新型隐球菌的抑菌活性及其可能的作用机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf040

Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior

{"title":"Characterization, antifungal activity, and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains.","authors":"Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior","doi":"10.1093/lambio/ovaf040","DOIUrl":"10.1093/lambio/ovaf040","url":null,"abstract":"This study aimed to characterize Melissa officinalis essential oil (EOMO) from a region in Northeast Brazil and evaluate its antifungal activity against Candida and Cryptococcus neoformans, by analyzing its action mechanism. EOMO was characterized using gas chromatography-mass spectrometry and the main chemical compounds were Geranial (14.10%) and (Z)-Nerolidol (17.75%). The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) against strains of Candida albicans, Candida parapsilosis, Candida krusei, Candida auris (MIC raging from 256 to 26.7 µg ml-1) and C. neoformans (MIC ranging from 64 to 32 µg ml-1). Flow cytometry and comet assays were employed to investigate EOMO's mechanism of action, which might be related to an increase in the production of reactive oxygen species and damage to fungal DNA. The chemical composition of EOMO from Northeast Brazil showed a higher content of (Z)-Nerolidol and has significant antifungal potential.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human gut microbiome and sleep across adulthood: associations and therapeutic potential. 人类肠道微生物群和成年期睡眠：关联和治疗潜力。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf043

Jacob Cavon, Melissa Basso, Kathrin Cohen Kadosh, Sean M Gibbons

引用次数: 0

Caffeine as a natural inhibitor of Salmonella biofilms in fruit juices. 咖啡因作为果汁中沙门氏菌生物膜的天然抑制剂。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf053

André Ricardo Peron Dos Santos, Bruna Camila Souza Lima, Gabriel José Couto, Luana de Carvalho, Lucília Rocha Magna, Matheus Henrique Nogueira, Mariana Lima Braga, Milena Matesco Carreteiro, Márcia Cristina Furlaneto, Luciana Furlaneto Maia

{"title":"Caffeine as a natural inhibitor of Salmonella biofilms in fruit juices.","authors":"André Ricardo Peron Dos Santos, Bruna Camila Souza Lima, Gabriel José Couto, Luana de Carvalho, Lucília Rocha Magna, Matheus Henrique Nogueira, Mariana Lima Braga, Milena Matesco Carreteiro, Márcia Cristina Furlaneto, Luciana Furlaneto Maia","doi":"10.1093/lambio/ovaf053","DOIUrl":"10.1093/lambio/ovaf053","url":null,"abstract":"Caffeine holds promise for applications in food safety due to its antioxidant and antibacterial properties. Given rising antimicrobial resistance, its natural antimicrobial potential is valuable for controlling foodborne pathogens and reducing reliance on synthetic preservatives. This study aimed to explore caffeine as an alternative to control Salmonella biofilms in fruit juice substrates. Salmonella Enteritidis and S. Typhimurium biofilm were developed in brain heart infusion (BHI) broth (control) and grape and apple juice before and after caffeine application. Biofilm inhibition was quantified by crystal violet staining, exopolysaccharide (EPS) production, and visualization through confocal and scanning electron microscopy. Swimming motility assays assessed caffeine's impact on bacterial motility. Both strains formed biofilms in the tested juices. The minimum inhibitory concentration (MIC) of caffeine was 9.37 mM mL-1 for S. Typhimurium and 18.75 mM mL-1 for S. Enteritidis. Biofilm inhibition was observed for treatments before and after caffeine application, with varying levels depending on the matrix. EPS production and inhibition were higher in biofilms formed in grape and apple juices compared to the control (BHI). Sub-inhibitory concentrations of caffeine reduced motility in both strains. These findings suggest that caffeine may be a promising approach to control Salmonella biofilms in the food industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a rapid diagnostic method for Dickeya dadantii, the bacterial canker pathogen of peach, using loop-mediated isothermal amplification. 建立一种环介导等温扩增快速诊断桃溃疡病病菌的方法。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf057

Ryoji Suzuki, Namiko Kirino, Ryotaro Nakamura, Yuta Uchida, Takashi Fujikawa

{"title":"Development of a rapid diagnostic method for Dickeya dadantii, the bacterial canker pathogen of peach, using loop-mediated isothermal amplification.","authors":"Ryoji Suzuki, Namiko Kirino, Ryotaro Nakamura, Yuta Uchida, Takashi Fujikawa","doi":"10.1093/lambio/ovaf057","DOIUrl":"https://doi.org/10.1093/lambio/ovaf057","url":null,"abstract":"Given that accurate diagnosis of bacterial canker in peach trees that cause rapid death is difficult based on disease symptoms alone, a rapid genetic diagnosis technique is required. In this study, we aimed to develop a rapid and efficient method to identify Dickeya dadantii, the causative agent, using the loop-mediated isothermal amplification (LAMP) method. Two types of DNA were extracted from the thin cambium slices of peach tree trunks and branches. Purified DNA was obtained using a commercial kit. Crude DNA was boiled and diluted with the Tris-HCl buffer. Then, 119 peach tree cambium samples were analyzed to detect D. dadantii using the previously reported polymerase chain reaction method and newly designed LAMP primers. Notably, LAMP exhibited equivalent or superior positivity rates compared to polymerase chain reaction for both DNA types, particularly crude DNA. Additionally, D. dadantii was detected in the resin formed on the peach tree trunks and branches. Overall, the developed method shows potential for the non-invasive diagnosis of infections and mitigation of infection risk for effective fruit tree management.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermophilic Pseudomonas aeruginosa strain Ch39 isolated from Chignahuapan hot springs in Puebla, Mexico. 墨西哥普埃布拉Chignahuapan温泉中分离的嗜热绿脓杆菌Ch39。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf059

Hugo G Castelán-Sánchez, Jennifer V Fernández Dodero, Jorge Rojas-Vargas, Fernando Martínez-Ocampo, Juan Manuel Hurtado-Ramírez, David I Ríos-Vázquez, Patricia Sánchez-Alonso, Candelario Vazquez-Cruz, Norma E Rojas-Ruiz

{"title":"Thermophilic Pseudomonas aeruginosa strain Ch39 isolated from Chignahuapan hot springs in Puebla, Mexico.","authors":"Hugo G Castelán-Sánchez, Jennifer V Fernández Dodero, Jorge Rojas-Vargas, Fernando Martínez-Ocampo, Juan Manuel Hurtado-Ramírez, David I Ríos-Vázquez, Patricia Sánchez-Alonso, Candelario Vazquez-Cruz, Norma E Rojas-Ruiz","doi":"10.1093/lambio/ovaf059","DOIUrl":"https://doi.org/10.1093/lambio/ovaf059","url":null,"abstract":"Thermophilic bacteria have specific metabolic specializations to survive at high temperatures. This study focuses on Pseudomonas aeruginosa strain Ch39, a new isolate from mineral-rich thermal water from Chignahuapan, Puebla, Mexico. Biochemical testing, whole genome sequencing, and antimicrobial resistance profiling of strain Ch39 yielded significant detailed results. Genome sequencing yielded a high-quality 6.68 Mb assembly with a GC content of 66.13%, and annotation identified 4 356 protein-coding genes, including heat shock and antibiotic resistance genes. Comparative analysis of growth kinetics with the reference strains ATCC 27853 and PAO1 showed that Ch39 exhibited good growth and thermotolerance, with viability at 45°C, due to putative genetic adaptations such as heat shock proteins. Antibiotic resistance profiling showed variability in resistance profiles and the presence of resistance genes. In particular, strain Ch39 showed increased minimum inhibitory concentrations for some of the antibiotics tested, such as tetracycline (>1000 µg/mL), compared to the control strains. The observations made here emphasize the thermoadaptation of the strain and its suitability as a reservoir for antibiotic-resistance genes. This study expands our understanding of the thermophilic adaptation of P. aeruginosa and its ecological and clinical significance.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing microbial functionalities and ecological roles in Rajpardi lignite mine: insights from metagenomics analysis. 揭示Rajpardi褐煤矿微生物功能和生态作用：来自宏基因组学分析的见解。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf048

Anjana Sanghani, Komal Antaliya, Rajesh Patel, Shailesh Dave, Devayani Tipre

{"title":"Revealing microbial functionalities and ecological roles in Rajpardi lignite mine: insights from metagenomics analysis.","authors":"Anjana Sanghani, Komal Antaliya, Rajesh Patel, Shailesh Dave, Devayani Tipre","doi":"10.1093/lambio/ovaf048","DOIUrl":"10.1093/lambio/ovaf048","url":null,"abstract":"The present study employs a metagenomics approach to evaluate microbial communities' ecological functions and potential within the Rajpardi lignite mine of Gujarat, India. Through whole genome shotgun sequencing on the Illumina Miseq platform, we obtained 10 071 318 sequences, which unveiled a diverse and abundant microbial community primarily composed of Proteobacteria, Acidobacteria, and Nitrospirae. Comprehensive taxonomic profiling and gene prediction was carried out using the SqueezeMeta pipline, which highlighted significant contributions to carbohydrate, amino acid, and energy metabolism. The detection of antimicrobial resistance and stress resistance genes, such as blaTEM and merA, suggests that these microbes possess the ability to adapt to harsh environmental conditions. Genome binning revealed species such as Acidiphilum sp. 20-67-58, emphasizing the nature of these communities as they adapted to an acidic environment. This finding highlights the crucial role of microbes in biogeochemical cycles, emphasizing their potential in bioremediation, pollutant degradation, and ecosystem restoration.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrocleys nymphoides (water poppy): a cosmopolitan aquatic plant with a phytochemical-rich extract exhibiting antimicrobial and antibiofilm activity against multidrug-resistant Staphylococcus. 水罂粟：一种世界性的水生植物，具有丰富的植物化学物质提取物，对多重耐药葡萄球菌具有抗菌和抗生物膜活性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf046

Marcos Toneto Francisco, Pedro Henrique de Jesus Barbosa, Faizan Ahmad, Sérgio Antônio Fernandes, Marcia Giambiagi-deMarval, Marisa Alves Nogueira Diaz, Ciro César Rossi

{"title":"Hydrocleys nymphoides (water poppy): a cosmopolitan aquatic plant with a phytochemical-rich extract exhibiting antimicrobial and antibiofilm activity against multidrug-resistant Staphylococcus.","authors":"Marcos Toneto Francisco, Pedro Henrique de Jesus Barbosa, Faizan Ahmad, Sérgio Antônio Fernandes, Marcia Giambiagi-deMarval, Marisa Alves Nogueira Diaz, Ciro César Rossi","doi":"10.1093/lambio/ovaf046","DOIUrl":"10.1093/lambio/ovaf046","url":null,"abstract":"Antimicrobial resistance among Staphylococcus species, including multidrug-resistant and biofilm-forming strains, poses a critical threat to global health, demanding innovative therapeutic solutions. In this context, this study explores the antimicrobial and antibiofilm potential of the aquatic plant Hydrocleys nymphoides as a promising alternative. Extracts from the plant's leaves and roots were obtained using solvents of increasing polarity and tested against five key pathogenic Staphylococcus species: S. aureus, S. epidermidis, S. haemolyticus, S. pseudintermedius, and S. coagulans. The hexane extract of H. nymphoides leaves showed the most notable activity, with inhibition zones of 9-17 mm and minimum inhibitory concentrations (MICs) as low as 0.8 mg/ml for certain strains. Subinhibitory concentrations of the extract significantly reduced biofilm formation in most strains, with reductions up to 46.9%. Gas chromatography-mass spectrometry revealed bioactive compounds such as linoleic acid, n-hexadecanoic acid, 9-octadecenal, eicosane, and tetratriacontane, known for their antimicrobial and antibiofilm properties. Although cytotoxicity was observed at concentrations near the MIC, lower concentrations were non-toxic, indicating potential for controlled therapeutic applications. These findings underscore the biotechnological value of H. nymphoides as a sustainable source of antimicrobial agents against multidrug-resistant Staphylococcus. This work emphasizes the critical role of phytotherapy in combating the escalating antimicrobial resistance crisis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Vibrio cholerae from the Jukskei River in Johannesburg, South Africa. 来自南非约翰内斯堡Jukskei河的霍乱弧菌的特征。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf036

Luyanda Mkhize, Musa Marimani, Sanelisiwe Thinasonke Duze

{"title":"Characterization of Vibrio cholerae from the Jukskei River in Johannesburg, South Africa.","authors":"Luyanda Mkhize, Musa Marimani, Sanelisiwe Thinasonke Duze","doi":"10.1093/lambio/ovaf036","DOIUrl":"10.1093/lambio/ovaf036","url":null,"abstract":"The current study aimed to isolate and characterize Vibrio cholerae isolated from the Jukskei River, one of the largest Rivers in Johannesburg, South Africa. Water samples collected from the Jukskei River were subjected to culture-based methods for the detection and isolation of V. cholerae. Twenty-four V. cholerae were isolated, confirmed using real-time PCR, and sequenced using the MInION portable nanopore-sequencing device. Reference-based genome assemblies were constructed from the raw reads using the EPI2ME software followed by bioinformatics analysis using the Centre for Genomic Epidemiology website. All the V. cholerae isolates isolated from the Jukskei River were classified as non-O1/non-O139 and none of the isolates harbored the cholera toxin gene, ctxA. All 24 V. cholerae isolates belonged to sequence type 741, virulent genes including toxR, vspD, als, hlyA, makA, and rtxA as well as the Vibrio pathogenicity island 2 were detected amongst the isolates. Antimicrobial resistance genes (parC, varG, and gyrA) were detected in 83% of isolates. Although V. cholerae non-O1/non-O139 are not associated with epidemic cholera they can still cause mild to life-threatening illnesses. Therefore, increased surveillance should be considered to better understand the public health risks to the local community.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global epidemiology of high priority and pandemic Pseudomonas aeruginosa in pets, livestock, wild, and aquatic animals: a systematic review and meta-analysis. 宠物、家畜、野生动物和水生动物中高度优先和大流行的铜绿假单胞菌的全球流行病学：系统回顾和荟萃分析。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf028

Idris Nasir Abdullahi, Sirine Mejri, Chukwudi Cresent Okwume, Nurudeen Adedeji Lawal, Olatunde A Olusegun, Rym Ben Sallem, Karim Ben Slama

{"title":"Global epidemiology of high priority and pandemic Pseudomonas aeruginosa in pets, livestock, wild, and aquatic animals: a systematic review and meta-analysis.","authors":"Idris Nasir Abdullahi, Sirine Mejri, Chukwudi Cresent Okwume, Nurudeen Adedeji Lawal, Olatunde A Olusegun, Rym Ben Sallem, Karim Ben Slama","doi":"10.1093/lambio/ovaf028","DOIUrl":"10.1093/lambio/ovaf028","url":null,"abstract":"High-priority and international high-risk Pseudomonas aeruginosa (PA) are emergent strains. Here, we performed the first systematic review and meta-analysis of studies that reported the frequency of PA, carbapenem (CARBR), and colistin (COLR) resistant PA in healthy and sick livestock (A), pets (B), wild (C), and aquatic animals (D) from January 2013 to January 2023, globally. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, 57 cross-sectional and eight prospective studies were found eligible. Random-effect models were used to determine the pooled prevalences. Phylogenetic analyses based on core-genome single nucleotide polymorphisms (SNPs) were performed using CSI Phylogeny 1.4. The overall pooled prevalence of PA, COLR-PA, and CARBR-PA were 18.6%, 4%, and 11.7%, respectively. Wild animals had the highest pooled prevalence of PA, 33.5%. The pooled prevalence of COLR was significantly higher in PA strains from sick than healthy animals (P < 0.0001). Furthermore, COLR was significantly highest in PA strains from wild animals, 9.8% [95% confidence interval (CI): 0.8-58.9], while CARBR was highest in PA strains from pets, 14.8% (95% CI: 6.7-29.8). Seven (ST235, ST111, ST233, ST244, ST357, ST308, and ST277) of the 10 high-risk CARBR-PA clones carrying ExoS/U were reported in animals. The SNP-based phylogenetic analysis of 23 genomes of CARBR-PA from animals obtained from publicly available databases revealed two closely related blaGES-carrying strains (<100 SNPs) in sick pets, suggesting a transmission event. A higher frequency of high-risk carbapenem than colistin-resistant PA strains exists in animals. These findings highlight the roles of different animal species, especially pets and wild animals in the persistence and dissemination of high-priority PA.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation potential of Pseudomonas aeruginosa to counteract Arsenite-induced phytotoxicity in Solanum lycopersicum cultivated within a contaminated agroecosystem. 铜绿假单胞菌对受污染农业生态系统栽培的番茄茄中砷酸盐诱导的植物毒性的生物修复潜力

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf027

Vivek Patel, Naga Rathna Supriya G

{"title":"Bioremediation potential of Pseudomonas aeruginosa to counteract Arsenite-induced phytotoxicity in Solanum lycopersicum cultivated within a contaminated agroecosystem.","authors":"Vivek Patel, Naga Rathna Supriya G","doi":"10.1093/lambio/ovaf027","DOIUrl":"10.1093/lambio/ovaf027","url":null,"abstract":"Trivalent arsenic (AsIII) is the most toxic form of arsenic, accumulates in plant systems through aquaporins, and inhibiting plant growth. This study focuses on mitigating the bioavailability of arsenite (AsIII) in agricultural soils through biological approaches. A potential AsIII tolerant bacterium Pseudomonas aeruginosa VS3 was isolated from contaminated soil. Subsequent analysis revealed that this strain can produce exopolysaccharides (EPS) and biofilms. Additionally, the strain exhibited production of plant growth promoting traits, incuding Indole-3-acetic acid (IAA), gibberellins, and silicon (Si) solubilisation. Biotransformation assay demonstrated that strain can oxidize AsIII to the less toxic arsenate (AsV) with conversion efficiency of 51%. Findings from the field trial proven that P. aeruginosa significantly reduced AsIII toxicity in Solanum lycopersicum and boosted plant growth under AsIII stress conditions. Additionally, inoculation with P. aeruginosa enhanced the activities of antioxidant enzymes (40% increase in peroxidase and a 17% increase in phenylalanine ammonia-lyase) compared to untreated controls under AsIII stress. The bacterial treatment reduced arsenic accumulation in root tissues, demonstrating P. aeruginosa VS3's potential as a bioremediation agent to alleviate arsenite stress and enhance plant growth.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0