Letters in Applied Microbiology最新文献_第5页

Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B. 重组酯酶 B 对耐亚胺培南铜绿假单胞菌的再敏感性和肠杆菌科细菌对头孢菌素的敏感性的恢复。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae118

Wirongrong Whangsuk, Punyawee Dulyayangkul, Suvit Loprasert, James M Dubbs, Paiboon Vattanaviboon, Skorn Mongkolsuk

{"title":"Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B.","authors":"Wirongrong Whangsuk, Punyawee Dulyayangkul, Suvit Loprasert, James M Dubbs, Paiboon Vattanaviboon, Skorn Mongkolsuk","doi":"10.1093/lambio/ovae118","DOIUrl":"10.1093/lambio/ovae118","url":null,"abstract":"Sphingobium sp. SM42 Esterase B (EstB) is an enzyme with a dual function in degrading dibutyl phthalate and catalyzing the cleavage of the C-S bond in C3-sidechains of the dihydrothiazine ring of cephalosporins, generating more active β-lactam derivatives. Global prokaryotic genome analysis revealed the existence of a gene identical to estB in Pseudomonas aeruginosa strain PS1 suggesting a horizontal gene transfer event involving estB. To investigate the effect of ectopic expression of EstB in the periplasm of P. aeruginosa and several Enterobacteriaceae on antibiotic susceptibility levels, plasmid, pEstB, carrying a recombinant EstB fused with the signal peptide from Escherichia coli outer membrane protein A (OmpA) for periplasmic localization was constructed. The expression of EstB in the periplasm of P. aeruginosa and the Enterobacteriaceae: E. coli, Klebsiella pneumoniae, and Salmonella enterica serovar Typhi, increased susceptibility to carbapenems and cephalosporins. EstB reversed the imipenem resistance of P. aeruginosa ΔmexS and restored the changes in susceptibility to cephalosporins conferred by the downregulation of the outer membrane proteins, OmpK35 and OmpK36, in K. pneumoniae ΔramR-ompK36 to wild-type level. The introduction of EstB to the periplasmic space of Gram-negative bacteria can increase carbapenem and cephalosporin susceptibility.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depth-dependent bacterial colonization on model chitin particles in the open ocean. 开阔海洋中甲壳素模型颗粒上随深度变化的细菌定殖。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae107

Cordelia Roberts, Kimberley Bird, Nathan Chrismas, Susan Hartman, Michael Cunliffe

{"title":"Depth-dependent bacterial colonization on model chitin particles in the open ocean.","authors":"Cordelia Roberts, Kimberley Bird, Nathan Chrismas, Susan Hartman, Michael Cunliffe","doi":"10.1093/lambio/ovae107","DOIUrl":"10.1093/lambio/ovae107","url":null,"abstract":"Sinking particles transport carbon from the surface to the deep ocean. Microbial colonization and remineralization are important ecosystem services constraining ocean biogeochemistry by recycling and redistributing nutrients from the surface to the deep ocean. Fragmentation of particles by zooplankton and the resulting colonization by microorganisms before ingestion, known as 'microbial gardening', allows for trophic upgrading and increased microbial biomass for detritivorous zooplankton. Using model chitin particles incubated with seawater collected from the surface, mesopelagic and bathypelagic depths in the Northeast Atlantic Ocean, we determined particle-attaching bacterial communities to identify general and depth-specific candidates of particle colonization. Comparison of particle-attached communities at the amplicon sequence variant level showed that bacteria found on surface particles were also colonizers in the bathypelagic, in line with sinking particles promoting vertical connectivity. Bathypelagic particle-attached communities were most diverse. We propose that some particle colonizers attach to the surface and sink out with the particle, whilst other colonizers are depth-specific. This suggests that candidates for particle colonization differ with depth, which may be important when considering the implications for the delivery of ecosystem services, including carbon cycling and the role they play for zooplankton grazers.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter. 新型光谱学方法揭示大肠杆菌发酵罐最佳培养条件。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae123

Jia Chen, Huakun Wu, Jingru Chen, Jia Zheng, Wen Liu, Meifang Yu

{"title":"Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter.","authors":"Jia Chen, Huakun Wu, Jingru Chen, Jia Zheng, Wen Liu, Meifang Yu","doi":"10.1093/lambio/ovae123","DOIUrl":"https://doi.org/10.1093/lambio/ovae123","url":null,"abstract":"Fermentation engineering is critical for mass-producing chemicals, food additives, and medicines, where optimal culture conditions maximize microbial growth and metabolite production. Although monitoring bacterial growth during fermentation is critical, there is a lack of a non-invasive and sensitive method to directly monitor the bacterial metabolism. In this paper, a novel optical monitoring method is proposed based on tunable diode laser absorption spectroscopy. First, the detecting system consisting of a laser, detection, a homemade board, and an incubator is established and verified to be able to monitor the metabolite production of CO2 in Escherichia coli through a 25-h detection period. Second, the quantitative growth rate analysis method is specified by calculating the threshold time (TT) intervals between consecutive dilution gradients, and the threshold with the least sum of residuals is chosen as the optimal threshold. Finally, alongside varied pH and temperature settings in a simulated fermenter, we elucidated the influence of these factors on E. coli metabolism curves and calculated the growth rates via TT, identifying 38°C as the optimal temperature and 7.0 as the optimal pH. This study presents a novel approach to reveal optimal culture conditions during fermentation holding promises for online real-time monitoring in the future.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"77 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and scaling up of extracellular polysaccharide production by submerged culture of Ganoderma lucidum on starch-containing medium using response surface methodology and laboratory bioreactors of various designs. 利用响应面方法学和各种设计的实验室生物反应器，优化和扩大灵芝在含淀粉培养基上的浸没培养生产胞外多糖的规模。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae115

Tatyana S Tikhomirova, Maxim R Taraskevich, Yuriy A Lepekhin, Marina P Shevelyova, Vitaliy A Nemashkalov

{"title":"Optimization and scaling up of extracellular polysaccharide production by submerged culture of Ganoderma lucidum on starch-containing medium using response surface methodology and laboratory bioreactors of various designs.","authors":"Tatyana S Tikhomirova, Maxim R Taraskevich, Yuriy A Lepekhin, Marina P Shevelyova, Vitaliy A Nemashkalov","doi":"10.1093/lambio/ovae115","DOIUrl":"10.1093/lambio/ovae115","url":null,"abstract":"Basidiomycetes, known for their production of bioactive compounds, traditionally use simple sugars for fermentation. However, their ability to degrade complex plant polysaccharides through enzyme secretion presents potential for the use of renewable raw materials. This study focused on the optimization of exopolysaccharide (EPS) production and efficient substrate consumption by Ganoderma lucidum using response surface methodology (RSM). Using an optimized medium containing 15 g⋅l-1 wheat starch, 0.375 g⋅l-1 NH4Cl, and 0.75 g⋅l-1 CaCl2 (C/N ratio of 40), a significant increase in EPS concentration from 121.1 ± 10.2 mg⋅l-1 to 229.0 ± 20.3 mg⋅l-1 and starch degradation degree (SDD) from 9.1% to 57.6% was achieved after 9 d of submerged cultivation. Scale-up experiments were conducted in both column and stirred tank bioreactors, employing submerged and immobilized cultivation modes. Submerged cultivation in the column bioreactor yielded the highest process desirability of 0.56, achieving EPS concentration of 192.5 ± 5.4 mg⋅l-1 and 60.2% SDD within 7 d. These results highlight the potential of the used column bioreactor for efficient and rapid EPS production. Notably, bioreactor experiments revealed local maxima in EPS content at specific time points, suggesting that cell wall degradation, potentially induced by shear stress, may contribute to the release of polysaccharides into the culture broth.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial gamma-aminobutyric acid synthesis: a promising approach for functional food and pharmaceutical applications. 微生物合成γ-氨基丁酸：功能性食品和制药应用的前景广阔的方法。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae122

Racheal Oluwayemisi Fashogbon, Oyindamola John Samson, Theresa Abimbola Awotundun, Afolake Atinuke Olanbiwoninu, Bukola Christiana Adebayo-Tayo

{"title":"Microbial gamma-aminobutyric acid synthesis: a promising approach for functional food and pharmaceutical applications.","authors":"Racheal Oluwayemisi Fashogbon, Oyindamola John Samson, Theresa Abimbola Awotundun, Afolake Atinuke Olanbiwoninu, Bukola Christiana Adebayo-Tayo","doi":"10.1093/lambio/ovae122","DOIUrl":"10.1093/lambio/ovae122","url":null,"abstract":"Gamma-aminobutyric acid (GABA) is a non-protein amino acid that is a main inhibitory neurotransmitter in the mammalian central nervous system. This mini-review emphasis on the microbial production of GABA and its potential benefits in various applications. Numerous microorganisms, including lactic acid bacteria, have been identified as efficient GABA producers. These microbes utilize glutamate decarboxylase enzymes to convert L-glutamate to GABA. Notable GABA-producing strains include Lactobacillus brevis, Lactobacillus plantarum, and certain Bifidobacterium species. Microbial GABA production offers numerous benefits over chemical synthesis, including cost-effectiveness, sustainability, and the potential for in situ production in fermented foods. Recent research has optimized fermentation conditions, genetic engineering approaches, and substrate utilization to enhance GABA yields. The benefits of GABA extend beyond its neurotransmitter role. Studies have shown its potential to reduce blood pressure, assuage anxiety, improve sleep quality, and improve cognitive function. These properties make microbial GABA production particularly attractive for developing functional foods, nutraceuticals, and pharmaceuticals. Future research directions include exploring novel GABA-producing strains, improving production efficiency, and investigating additional health benefits of microbially produced GABA.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the probiotic and functional properties of Enterococcus faecalis AQ10 isolated from chicken cecum. 鸡盲肠粪肠球菌AQ10的益生菌特性及功能特性研究

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae116

Haichang Yin, Xinyu Zhang, Xinjie Jiang, Di Liu

{"title":"Characterization of the probiotic and functional properties of Enterococcus faecalis AQ10 isolated from chicken cecum.","authors":"Haichang Yin, Xinyu Zhang, Xinjie Jiang, Di Liu","doi":"10.1093/lambio/ovae116","DOIUrl":"10.1093/lambio/ovae116","url":null,"abstract":"Lactic acid bacteria are considered the safest alternative to antibiotics and have a broad range of applications in animal husbandry. Enterococcus faecalis is a primary component of the chicken gut microbiota; it is known for its ability to regulate intestinal microbial balance and its probiotic functions. In this study, E. faecalis strain AQ10 from isolated from was the chicken cecum and used a combination of whole-genome sequencing and phenotypic analyses to investigate its probiotic properties. Whole-genome sequencing revealed that the genome length of E. faecalis AQ10 is 2.98 Mbp, with an average guanine-cytosine content of 38.12%, and includes 2832 protein-coding genes. Genome mining tools were used to identify an antimicrobial compound gene cluster. Additionally, E. faecalis AQ10 exhibited probiotic characteristics in vivo and antibacterial effects in vitro, with organic acids potentially contributing to its anti-Klebsiella pneumoniae activity. Metabolomics analyses revealed that the supernatant of E. faecalis AQ10 contained seven organic acids. In conclusion, E. faecalis AQ10 demonstrates significant probiotic potential and may be a suitable candidate for use in livestock and poultry breeding.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviation of Helicobacter pylori- or aspirin-induced gastritis and neuroinflammation in mice by Lactococcus lactis and Bifidobacterium longum. 乳球菌和长双歧杆菌缓解幽门螺旋杆菌或阿司匹林诱发的小鼠胃炎和神经炎症

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae128

Jung-Woo Shin, Yoon-Jung Shin, Dong-Yun Lee, Dong-Hyun Kim

{"title":"Alleviation of Helicobacter pylori- or aspirin-induced gastritis and neuroinflammation in mice by Lactococcus lactis and Bifidobacterium longum.","authors":"Jung-Woo Shin, Yoon-Jung Shin, Dong-Yun Lee, Dong-Hyun Kim","doi":"10.1093/lambio/ovae128","DOIUrl":"10.1093/lambio/ovae128","url":null,"abstract":"Helicobacter pylori (HP) causes gastritis and peptic ulcer. Therefore, we examined whether probiotics Lactococcus lactis P135 and Bifidobacterium longum P142, which inhibited HP growth by 37.9% and 35.3%, respectively, and HP-induced IL-8 expression in KATO III cells by 68.6% and 63.1%, respectively, compared to those of normal controls, could mitigate HP-induced gastritis and psychiatric disorder in mice. Oral administration of P135 and/or P142 alleviated HP- or aspirin-induced gastritis, colitis, neuroinflammation, and depression/cognitive impairment-like behavior. They also suppressed HP infection, neutrophil infiltration, and NF-κB activation in the stomach and TNF-α expression and NF-κB activation in the colon and hippocampus. of P135 and/or P142 alleviated HP- or aspirin-induced gut dysbiosis: they decreased Lachnospiracease, Helicobacteriaceae, and Akkermansiaceae populations and increased Bacteroidaceae and Muribaculaceae populations. These findings suggest that HP growth/inflammation-inhibitory P135 and/or P142 may alleviate gut inflammation (gastritis and colitis) and neuroinflammation through the suppression of neutrophil infiltration, NF-κB activation, and HP growth, thereby leading to the attenuation of systemic inflammation and psychiatric disorder.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating gut microbiota for treating antibiotic-associated diarrhea from Clostridium difficile infection: insights from Lizhong decoction and its polysaccharide component. 调节肠道菌群治疗难辨梭菌感染引起的抗生素相关性腹泻：来自理中汤及其多糖成分的见解。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae121

XinQian Rong, QingLong Shu

{"title":"Modulating gut microbiota for treating antibiotic-associated diarrhea from Clostridium difficile infection: insights from Lizhong decoction and its polysaccharide component.","authors":"XinQian Rong, QingLong Shu","doi":"10.1093/lambio/ovae121","DOIUrl":"10.1093/lambio/ovae121","url":null,"abstract":"To investigate whether the polysaccharide component of the Traditional Chinese Medicine (TCM) formula Lizhong decoction (LZD) has therapeutic effects and regulates gut microbiota in antibiotic-associated diarrhea (AAD) related to Clostridium difficile infection, a polysaccharide was extracted from LZD, consisting of rhamnose, five monosaccharides, arabinose, galactose, glucose, and galacturonic acid in a molar ratio of 1.12:8.49:4.06:80.67:5.66. An AAD model related to C. difficile infection was established using clindamycin gavage and oral colonization with C. difficile. Subsequently, oral treatment experiments with LZD polysaccharides and different doses of the decoction were conducted. The results indicated that the polysaccharide showed the best therapeutic effect on AAD related to C. difficile infection, with post-treatment gut microbiota clustering closest to the normal control group. The polysaccharide from LZD has therapeutic effects on AAD related to C. difficile infection, possibly achieved through the regulation of gut microbiota. It modulates the dominant gut microbiota, specifically increasing the abundance of beneficial bacteria by adjusting the ratio of Bacteroidetes and Firmicutes, thereby reversing the dysbiosis caused by C. difficile infection and antibiotics. Thus, LZD polysaccharide may be one of the important active components for gut microbiota regulation in this TCM formula.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Lactiplantibacillus plantarum for treatment of Salmonella infection in mice. 分离植物乳杆菌以治疗小鼠沙门氏菌感染。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae114

Silu Zhang, Tian Liu, Xiaoli Zhou, Jun Wang, Tengxun Zhang, Guoxun Xiao, Song Huang, Chitong Rao, Xin Teng

{"title":"Isolation of Lactiplantibacillus plantarum for treatment of Salmonella infection in mice.","authors":"Silu Zhang, Tian Liu, Xiaoli Zhou, Jun Wang, Tengxun Zhang, Guoxun Xiao, Song Huang, Chitong Rao, Xin Teng","doi":"10.1093/lambio/ovae114","DOIUrl":"10.1093/lambio/ovae114","url":null,"abstract":"Salmonella infections, often acquired through contaminated food or water, pose significant health risks, particularly amid rising antibiotic resistance and the adverse effects of conventional treatments on gut microbiota. This study isolated the probiotic Lactiplantibacillus plantarum GOLDGUT-LP618 from healthy human fecal samples, demonstrating its potential against Salmonella enterica. Lactiplantibacillus plantarum LP618 produced high levels of short-chain fatty acids (SCFAs) and exhibited robust inhibition of Salmonella in vitro. The strain showed resilience in gastric and bile environments, effectively curbing Salmonella growth. Post-infection treatment with L. plantarum LP618 significantly alleviated diarrhea, improved body weight, and moderated inflammatory responses. Histological analysis indicated restoration of intestinal structure, suggesting protection against mucosal damage. These results highlight L. plantarum LP618 as a promising probiotic candidate, offering a safe and effective alternative to antibiotics for managing Salmonella infections and promoting recovery in affected individuals.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the efficacy of postbiotics derived from Lactobacillus plantarum on antibiotic resistance genes in nosocomial pathogens such as Enterococcus faecalis and Pseudomonas aeruginosa. 评估植物乳杆菌衍生后生制剂对粪肠球菌和铜绿假单胞菌等医院病原菌耐药基因的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-12-02 DOI: 10.1093/lambio/ovae127

Javad Nezhadi, Ali Ahmadi

{"title":"Assessing the efficacy of postbiotics derived from Lactobacillus plantarum on antibiotic resistance genes in nosocomial pathogens such as Enterococcus faecalis and Pseudomonas aeruginosa.","authors":"Javad Nezhadi, Ali Ahmadi","doi":"10.1093/lambio/ovae127","DOIUrl":"10.1093/lambio/ovae127","url":null,"abstract":"This study investigated the antibacterial and anti-biofilm properties of postbiotics derived from Lactobacillus plantarum and their effect on the expression of antibiotic resistance genes (ermB and blaKPC) in Enterococcus faecalis and Pseudomonas aeruginosa, respectively. Cell-free supernatants (CFSs) were analyzed through gas chromatography-mass spectrometry (GC-MS), which showed that butyric acid (14.31%) was the major compound, other metabolites present in CFSs included lactic acid (5.94%), hdroxyacetone (5,21%), benzoic acid (3.12%), Pyrrolo[1,2-a] pyrazine-1,4-dione (1.91%), 2,3-Butanediol (1.04%), and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (0.73.%). To investigate the effect of postbiotics on bacterial growth and biofilm formation, minimal inhibitory concentration (MIC) and microtiter plate assays were used. MIC results showed that resistant En. faecalis and P. aeruginosa can grow at concentrations of 2.5 and 5 mg/ml, respectively, after exposure to postbiotics. Furthermore, the microtiter plate results showed that postbiotics significantly reduced biofilm formation: 51%, 45%, and 39% in En. faecalis and 46%, 38%, and 27% in P. aeruginosa at different concentrations. Real-time polymerase chain reaction also confirmed the reduction of resistance genes (ermB; P = 0.007 and blaKPC; P = 0.02) expression. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that the cell survival rate was 80%. These findings suggest that postbiotics from L. plantarum may be a promising approach for combating bacterial growth, biofilm formation, and antibiotic resistance.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0