Letters in Applied Microbiology最新文献_第5页

Bacteria as anticancer agents: bioactive metabolites, engineered platforms, and translational mechanisms. 细菌作为抗癌剂：生物活性代谢物、工程平台和转化机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-06-12 DOI: 10.1093/lambio/ovag050

Manel Gharbi, Mohamed Salah Abbassi

{"title":"Bacteria as anticancer agents: bioactive metabolites, engineered platforms, and translational mechanisms.","authors":"Manel Gharbi, Mohamed Salah Abbassi","doi":"10.1093/lambio/ovag050","DOIUrl":"10.1093/lambio/ovag050","url":null,"abstract":"Bacteria represent a vast and underexplored reservoir of bioactive compounds with significant anticancer potential. Numerous bacterial taxa, particularly actinomycetes, Bacillus, Pseudomonas, and marine-derived species, produce structurally diverse metabolites exhibiting cytotoxic, cytostatic, pro-apoptotic, immunomodulatory, and anti-angiogenic activities against cancer cells. Clinically established agents such as actinomycin D and bleomycin highlight the therapeutic relevance of bacterial natural products, while recent discoveries continue to expand the repertoire of bioactive polyketides, peptides, alkaloids, and proteins. These compounds act through multiple mechanisms, including DNA intercalation, induction of apoptosis, cell cycle arrest, metabolic disruption, and modulation of the tumor microenvironment. Advances in metagenomics, genome mining, and synthetic biology have enabled the identification and activation of previously silent biosynthetic gene clusters, significantly enhancing drug discovery potential. In addition to metabolite-based anticancer agents, advances in synthetic biology have enabled the development of engineered bacterial platforms capable of selectively colonizing tumors, delivering therapeutic molecules, and activating prodrug therapies within the tumor microenvironment. Despite ongoing challenges related to toxicity, limited yield, selectivity, and clinical translation, bacterial-derived compounds remain a promising frontier in oncology. This review summarizes bacterial sources, bioactive metabolites, molecular mechanisms, preclinical and clinical applications, and future prospects for developing effective and safe anticancer strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic epidemiology and antimicrobial resistance of diarrheagenic Escherichia coli isolated from kindergarten children without diarrhoea symptoms in Hengyang, China. 衡阳市无腹泻症状幼儿园致泻性大肠杆菌基因组流行病学及耐药性分析

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-06-12 DOI: 10.1093/lambio/ovag051

Qiwei Shi, Qiwen Zhou, Ping Lou, Qiang Luo, Jie Li, Yali Zhou, Bo Pang, Huiling Li

{"title":"Genomic epidemiology and antimicrobial resistance of diarrheagenic Escherichia coli isolated from kindergarten children without diarrhoea symptoms in Hengyang, China.","authors":"Qiwei Shi, Qiwen Zhou, Ping Lou, Qiang Luo, Jie Li, Yali Zhou, Bo Pang, Huiling Li","doi":"10.1093/lambio/ovag051","DOIUrl":"10.1093/lambio/ovag051","url":null,"abstract":"Asymptomatic carriage of diarrheagenic Escherichia coli (DEC) in kindergarten children without diarrhoea symptoms represents an important yet underexplored component of community transmission. This study aimed to investigate the prevalence, genomic epidemiology, and antimicrobial susceptibility of DEC recovered from kindergarten children without diarrhoea symptoms in Hengyang, China. Faecal samples (n=150) were collected from kindergarten children without diarrhoea symptoms in Hengyang City in 2024. Molecular screening of these samples was performed using multiplex real-time polymerase chain reaction (PCR) targeting 16 enteric pathogens. PCR-positive samples were subjected to bacterial isolation, antimicrobial susceptibility testing, and whole-genome sequencing. Overall, 37 samples (24.7%) were PCR-positive for at least 1 enteric pathogen. DEC was the only pathogen group successfully recovered by culture, yielding 20 isolates, including 12 enteroaggregative Escherichia coli (EAEC), 7 enteropathogenic Escherichia coli and 1 enterotoxigenic Escherichia coli. Core genome multilocus sequence typing identified a highly conserved EAEC cluster comprising four isolates with zero allelic differences, alongside additional closely related isolates. Antimicrobial susceptibility testing revealed resistance to nalidixic acid, streptomycin, and ampicillin, while most isolates remained susceptible to critically important antibiotics. Genomic surveillance revealed that kindergarten children without diarrhoea asymptomatically carry genetically diverse DEC, including closely related EAEC lineages, underscoring the epidemiological relevance of asymptomatic carriage in community settings beyond clinically apparent diarrhoeal disease.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria surface charge varies across species and is independent of cell wall type. 细菌表面电荷在不同种类之间是不同的，并且与细胞壁类型无关。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-06-12 DOI: 10.1093/lambio/ovag048

Brittany J Carnathan, Christie M Sayes

{"title":"Bacteria surface charge varies across species and is independent of cell wall type.","authors":"Brittany J Carnathan, Christie M Sayes","doi":"10.1093/lambio/ovag048","DOIUrl":"10.1093/lambio/ovag048","url":null,"abstract":"Variation in the surface charge of the cell envelope across bacterial species is not well understood. The absence of systematic investigations comparing taxonomically diverse bacterial species has led to confusion about the influence of cell wall type (i.e. Gram-negative or Gram-positive bacteria) on surface charge. In this study, dynamic light scattering (DLS) was used to determine surface charge via zeta potential measurements of 12 phylogenetically diverse bacterial species spanning four bacterial phyla. Surface charge of six Gram-positive species (Bacillus subtilis, Streptococcus thermophilus, Paenibacillus polymyxa, Kocuria rosea, Mycobacterium smegmatis, and Streptomyces albus) and six Gram-negative species (Chryseobacterium shigense, Sphingobacterium multivorum, Flavobacterium pectinovorum, Escherichia coli, Stenotrophomonas maltophilia, and Pseudomonas putida) were characterized. Results show that surface charge is not dependent on cell wall type but instead varies across diverse species, as there is significant overlap between the surface charge means of the Gram-positive and Gram-negative bacterial groups. The methodology used in this study provides a consistent, high-throughput technique for assessing surface charge. Data presented herein suggest that routine surface charge measurements are informative for investigations of interactions between bacteria and other systems, such as antimicrobial therapeutics and water purification systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Lyme disease in a small urban city through wastewater detection of Borrelia burgdorferi. 利用污水检测伯氏疏螺旋体监测小城市莱姆病。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-05-11 DOI: 10.1093/lambio/ovag046

Sharon C Kosgei, Rylee N Reagan, Olivia N Birch, Justin C Greaves

{"title":"Monitoring Lyme disease in a small urban city through wastewater detection of Borrelia burgdorferi.","authors":"Sharon C Kosgei, Rylee N Reagan, Olivia N Birch, Justin C Greaves","doi":"10.1093/lambio/ovag046","DOIUrl":"10.1093/lambio/ovag046","url":null,"abstract":"Lyme disease remains the most reported vector-borne disease in the United States, yet traditional surveillance methods rely heavily on clinical diagnosis and laboratory confirmation, both of which are subject to underreporting and diagnostic limitations. This study evaluated the feasibility of wastewater-based epidemiology (WBE) as a complementary surveillance tool for monitoring the Lyme disease causing agent, Borrelia burgdorferi in a small urban community. It was conducted between 2023 and 2025 with 92 influent wastewater samples collected from two municipal treatment facilities in Bloomington, Indiana. Samples were concentrated using 0.45 µm pore size electronegative membrane filtration and analyzed via digital PCR for B. burgdorferi, with crAssphage quantified as a fecal normalization marker. Borrelia burgdorferi was detected intermittently at low concentrations, with peak values reaching 3649 gene copies/L. Detection exhibited pronounced seasonal variability, with the highest positivity occurring in fall (62.5%) and no detections observed in spring. No statistically significant differences were observed between treatment plants. Our findings indicate that B. burgdorferi can be detected in municipal wastewater and that detection patterns align with known seasonal trends in Lyme disease transmission. Despite low and intermittent detections, WBE may serve as a useful complementary population- level surveillance tool for B. burgdorferi in small urban systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fate of Clostridioides difficile spores in brain heart infusion broth and selected foods. 艰难梭菌孢子在脑心灌注（BHI）肉汤和选定食物中的命运。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-05-11 DOI: 10.1093/lambio/ovag043

Pilar Marcos, Paul Whyte, Declan Bolton

引用次数: 0

One-pot CRISPR/Cas12b-LAMP platform for dual-mode detection of Pasteurella multocida. 多杀性巴氏杆菌双模检测的CRISPR/Cas12b-LAMP单锅平台

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-05-11 DOI: 10.1093/lambio/ovag044

Ningyuan Zhong, Mengdi Wang, Wei Jiang, Guoxin Li, Jinfeng Miao, Huifang Yin, Phouthapane Vanhnaseng, Jiansen Gong, Zugong Yu, Xiangan Han

{"title":"One-pot CRISPR/Cas12b-LAMP platform for dual-mode detection of Pasteurella multocida.","authors":"Ningyuan Zhong, Mengdi Wang, Wei Jiang, Guoxin Li, Jinfeng Miao, Huifang Yin, Phouthapane Vanhnaseng, Jiansen Gong, Zugong Yu, Xiangan Han","doi":"10.1093/lambio/ovag044","DOIUrl":"10.1093/lambio/ovag044","url":null,"abstract":"Pasteurella multocida is a significant bacterial pathogen that poses a significant threat to public health and causes substantial economic losses. Existing detection methods for P. multocida have limitations, including time-consuming and technically complex methods. Here, we describe a simple and accurate detection platform that combines loop-mediated isothermal amplification (LAMP) with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12b system. By using heparin sodium to modulate the cis-cleavage activity of Cas12b, we developed a one-pot LAMP-CRISPR/Cas12b assay in a single closed tube. The assay achieved a detection limit of 5.0 × 101 CFU ml-1 and showed no cross-reactivity with other bacterial species, indicating high sensitivity and specificity. Furthermore, we validated the clinical utility of the platform using milk samples artificially contaminated with P. multocida, which successfully detected P. multocida in the LAMP-CRISPR/Cas12b results. In summary, this study establishes a novel and robust detection system for P. multocida and highlights its potential for nucleic acid-based diagnostics in practical applications.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in PCR-based detection of Escherichia coli. 基于pcr的大肠杆菌检测研究进展。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-05-11 DOI: 10.1093/lambio/ovag045

Bohan Hu, Chuanjin Cui, Jingdong Bo, Qian Li, Shubao Wang

引用次数: 0

Chemical composition and antimicrobial efficacy of essential oils from Cuminum cyminum leaves and seeds for preserving Sardina Pilchardus and Pagellus erythrinus. 沙丁鱼和红颡鱼保鲜用孜然叶和种子精油的化学成分及抑菌效果。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag037

Lamyae Mehane, Ibrahim Sadougui, Meryem Idrissi Yahyaoui, Mohamed Taibi, Amine Elbouzidi, Reda Bellaouchi, Bouchra El Guerrouj, Rachid Sabbahi, Viktória Lilla Balázs, Aleksandar Széchenyi, Belkheir Hammouti, Abdeslam Asehraou

{"title":"Chemical composition and antimicrobial efficacy of essential oils from Cuminum cyminum leaves and seeds for preserving Sardina Pilchardus and Pagellus erythrinus.","authors":"Lamyae Mehane, Ibrahim Sadougui, Meryem Idrissi Yahyaoui, Mohamed Taibi, Amine Elbouzidi, Reda Bellaouchi, Bouchra El Guerrouj, Rachid Sabbahi, Viktória Lilla Balázs, Aleksandar Széchenyi, Belkheir Hammouti, Abdeslam Asehraou","doi":"10.1093/lambio/ovag037","DOIUrl":"10.1093/lambio/ovag037","url":null,"abstract":"This study examines the chemical composition and antimicrobial activity of essential oils (EOs) extracted from cumin (Cuminum cyminum L.) seeds and leaves harvested in the Bouarfa region of Morocco, focusing on their potential application in food preservation. Gas chromatography-mass spectrometry (GC-MS) and thin layer chromatography-bioautography analyses were employed to identify key antimicrobial components in both seed and leaf EOs. GC-MS revealed that cumin seed EO was dominated by cuminaldehyde (31.07%), α-Thujenal (19.86%), and 2-Caren-10-al (18.51%), while the leaf EO contained cuminaldehyde (32.45%), γ-Terpinene (15.46%), and α-Thujenal (12.89%) as the major components. Bioautography confirmed strong antimicrobial activity, particularly for cuminaldehyde, α-Thujenal, and 2-Caren-10-al, with inhibition zones ranging from 7.5 to 16.3 mm against both bacterial and fungal pathogens. The minimum inhibitory concentrations for these compounds ranged from 0.39 to 1.56 mg mL-1, while the minimum bactericidal concentrations (MBCs) varied from 1.56 to 6.25 mg mL-1, indicating potent antibacterial effects. Overall, these findings highlight the potential of cumin-derived compounds as natural preservatives capable of extending the shelf life of sardine (Sardina pilchardus) and white fish (Pagellus erythrinus) by inhibiting microbial growth. Their application in fish-based matrices demonstrates promising potential for preventing spoilage and enhancing food safety.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivation of Mycobacterium avium ssp. paratuberculosis in cattle manure by lactic acid fermentation is dependent on carbohydrate source. 鸟分枝杆菌的失活。乳酸发酵牛粪中的副结核与碳水化合物来源有关。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag015

Hannah Halm, Heike Köhler, Charlotte Schröder, Hendrik Scheinemann

{"title":"Inactivation of Mycobacterium avium ssp. paratuberculosis in cattle manure by lactic acid fermentation is dependent on carbohydrate source.","authors":"Hannah Halm, Heike Köhler, Charlotte Schröder, Hendrik Scheinemann","doi":"10.1093/lambio/ovag015","DOIUrl":"10.1093/lambio/ovag015","url":null,"abstract":"Mycobacterium avium ssp. paratuberculosis (MAP) is an intestinal pathogen which is excreted fecally and can be spread in the environment through contaminated manure. Lactic acid fermentation (LAF) was evaluated as a method to inactivate MAP in cattle manure (CM). As carbohydrate (CHO) sources oats and saccharose (Sac) were used. After mixing, manure was incubated at 21°C for 8 weeks. The microbial shift was determined using cultural methods. The results showed different suitability of the selected CHOs for inactivation of MAP by LAF. Using squeezed oats as an additive, culturable MAP was reduced to below the detection limit after 35 and 42 days of fermentation. Additional Sac decreased the reduction time to 21 days. With Sac only addition, inactivation of MAP was not successful and bacterial counts did not differ from the negative control. Detection of IS900 genome fragments using real time-polymerase chain-reaction (PCR) showed that the number of gene copies in the manure did not decrease during the course of the experiments. This study showed that LAF is a valuable option for decontaminating CM with natural resources in the case of paratuberculosis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of stale bread into protein-rich biomass: an optimized Rhizopus oryzae fermentation strategy and characterization study. 变质面包转化为富含蛋白质的生物质：一种优化的米根霉发酵策略和特性研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag034

Burcu Kaya, Özlem Şahiner, Gökçe Örüntaş, Erol Kestek, Harun Efe Bozaslan, Seda Savranoğlu Kulabaş, Tugba Boyunegmez Tumer, Onur Güneşer, Yonca Karagül Yüceer

{"title":"Valorization of stale bread into protein-rich biomass: an optimized Rhizopus oryzae fermentation strategy and characterization study.","authors":"Burcu Kaya, Özlem Şahiner, Gökçe Örüntaş, Erol Kestek, Harun Efe Bozaslan, Seda Savranoğlu Kulabaş, Tugba Boyunegmez Tumer, Onur Güneşer, Yonca Karagül Yüceer","doi":"10.1093/lambio/ovag034","DOIUrl":"10.1093/lambio/ovag034","url":null,"abstract":"Bread is one of the most widely consumed and most wasted foods worldwide. It contains essential nutrients; bread provides an environment for microbial growth. This study aims to maximize biomass and protein production by Rhizopus oryzae through fermentation optimization. The RNA concentration of the biomass produced under optimal conditions was quantified. The cytotoxicity was evaluated in HT-29, HUVEC, and HepG2. A dilute acid-heat pretreatment, conducted with 2.5% H2SO4 (v v-1) and 5% (w v-1) stale bread at 121°C for 2 min (4.83 g l-1 fermentable sugars, 0.86 mg 100 ml-1 hydroxymethyl furfural). Then, enzymatic hydrolysis of the pretreated substrate increased fermentable sugar, with an optimum enzyme concentration of 0.05% and a hydrolysis time of 2 h. Under the optimized fermentation conditions (35°C, 3 days, 200 rpm, and pH 4.5), a biomass yield of 7.06 g l-1 and a protein content of 36.76% were obtained. The RNA content of biomass was determined to be within 0.24%-0.35%. The absence of adverse effects on cellular proliferation and growth indicates the biocompatibility of the produced biomass. The results suggest that R. oryzae cultivated on stale bread is a promising candidate for functional food applications with enhanced protein content.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147458287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0