Letters in Applied Microbiology最新文献_第5页

A review of the environmental spread of Salmonella enterica serovars through water in Africa.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf005

Akeem Ganiyu Rabiu, Oyinkansola Fadiji, Ayodele Timilehin Adesoji, Simiat Olanike Jimoh, Sohyun Cho

{"title":"A review of the environmental spread of Salmonella enterica serovars through water in Africa.","authors":"Akeem Ganiyu Rabiu, Oyinkansola Fadiji, Ayodele Timilehin Adesoji, Simiat Olanike Jimoh, Sohyun Cho","doi":"10.1093/lambio/ovaf005","DOIUrl":"10.1093/lambio/ovaf005","url":null,"abstract":"Salmonella is an enteric pathogenic bacterium in mammals that thrives in sewage, soil, and aquatic environments because of its wide ecological adaptability. The spread of Salmonella infection is associated with a lack of clean water, poor hygiene, and poor sanitation in developing countries. However, the input of Salmonella-contaminated surface water and groundwater in the environmental dissemination of antimicrobial-resistant Salmonella is obscure outside developed countries. This study aimed to assess literature that described Salmonella from groundwater, surface water, and treated water in Africa and profiled the bacteria for antimicrobial resistance (AMR). We seek to highlight the pitfalls in S. enterica identification schemes and determine the entry routes of S. enterica into surface water and groundwater to comprehend the spread patterns of S. enterica through the water ecosystem. Salmonella was frequently recovered not only from groundwater but also from unprotected springs and river water in Africa. However, the insufficiency of standard methods limited the speciation of the bacteria and only a few studies determined the isolates' AMR patterns. Integrating the Salmonella surveillance in environmental and treated water into the global Salmonella surveillance can assist in controlling typhoid fever spread and generally reduce the burden of waterborne infections in Africa.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hospital environment as reservoir of Pseudomonas aeruginosa in human cases: a molecular epidemiology investigation in a hospital setting in central Italy.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf019

Adele Lombardi, Manuela Tamburro, Michela Lucia Sammarco, Giancarlo Ripabelli

{"title":"Hospital environment as reservoir of Pseudomonas aeruginosa in human cases: a molecular epidemiology investigation in a hospital setting in central Italy.","authors":"Adele Lombardi, Manuela Tamburro, Michela Lucia Sammarco, Giancarlo Ripabelli","doi":"10.1093/lambio/ovaf019","DOIUrl":"10.1093/lambio/ovaf019","url":null,"abstract":"Pseudomonas aeruginosa is one of the main causes of hospital infections that are difficult to manage because of multidrug resistance (MDR). The aim of this study was to describe a molecular investigation on 19 clinical and 14 isolates from the environment in the Hub hospital of the Molise region, central Italy. Antimicrobial susceptibility was evaluated using BD Phoenix™ Automated Microbiology System. Pulsed-field gel electrophoresis (PFGE) with SpeI, enterobacterial repetitive intergenic consensus (ERIC-PCR), and random amplified polymorphic DNA-PCR were performed for genotyping. All 33 P. aeruginosa showed MDR phenotype. PFGE had 0.99 discriminatory power, underlining high heterogeneity among the strains. The genetic relatedness between two human isolates (H12 and H15) from neonatal intensive care (NICU) and one environmental strain (E1) collected from siphon of sink in the delivery room was noticeable, as well as between one strain from faucet in NICU (E2) and siphon sink (E5) from delivery room. The link between H12, H15, and E1 strains was corroborated by ERIC-PCR showing epidemiological concordance, although with a lower discriminatory power. The study findings strengthened the critical correlation between clinical P. aeruginosa and environment, according to previous molecular surveys on outbreaks occurred in Italy.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructed wetland-microbial fuel cell (CW-MFC) mediated bio-electrodegradation of azo dyes from textile wastewater.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf010

Nikita Kundu, Smriti Yadav, Ananya Bhattacharya, G K Aseri, Neelam Jain

{"title":"Constructed wetland-microbial fuel cell (CW-MFC) mediated bio-electrodegradation of azo dyes from textile wastewater.","authors":"Nikita Kundu, Smriti Yadav, Ananya Bhattacharya, G K Aseri, Neelam Jain","doi":"10.1093/lambio/ovaf010","DOIUrl":"10.1093/lambio/ovaf010","url":null,"abstract":"Azo dyes constitute 60%-70% of commercially used dyes and are complex, carcinogenic, and mutagenic pollutants that negatively impact soil composition, water bodies, flora, and fauna. Conventional azo dye degradation techniques have drawbacks such as high production and maintenance costs, use of hazardous chemicals, membrane clogging, and sludge generation. Constructed wetland-microbial fuel cells (CW-MFCs) offer a promising sustainable approach for the bio-electrodegradation of azo dyes from textile wastewater. CW-MFCs harness the phytodegradation capabilities of wetland plants like Azolla, water hyacinth, and Ipomoea, along with microalgae such as Nostoc, Oscillatoria, Chlorella, and Anabaena, to break down azo dyes into aromatic amines. These intermediates are then reduced to CO2 and H2O by microalgae in the fuel cells while simultaneously generating electricity. CW-MFCs offer advantages including low cost, sustainability, and use of renewable energy. The valorization of the resulting algal and plant biomass further enhances the sustainability of this approach, as it can be used for biofuel production, nutraceuticals, pharmaceuticals, and bio-composting. Implementing CW-MFCs as a tertiary treatment step in textile industries aligns with the circular economy concept and contributes to achieving several sustainable development goals.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genomic characterization of Levilactobacillus brevis YT108: a potential probiotic strain capable of metabolizing xylo-oligosaccharides.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf014

Yang Chen, Jun Zhou, Xin Li, Xin-Yao Li, Shi-Xiu Qiu, Zhen-Ying Xu, Jian-Xia Yang, Yuan-Ting Zhu, Xin-Rui Zhang, Lang Yan

{"title":"Phenotypic and genomic characterization of Levilactobacillus brevis YT108: a potential probiotic strain capable of metabolizing xylo-oligosaccharides.","authors":"Yang Chen, Jun Zhou, Xin Li, Xin-Yao Li, Shi-Xiu Qiu, Zhen-Ying Xu, Jian-Xia Yang, Yuan-Ting Zhu, Xin-Rui Zhang, Lang Yan","doi":"10.1093/lambio/ovaf014","DOIUrl":"10.1093/lambio/ovaf014","url":null,"abstract":"Levilactobacillus brevis YT108, identified for its ability to metabolize prebiotic xylo-oligosaccharides (XOS), emerges as a candidate for probiotic use in synbiotic food formulations. This study aimed to investigate the metabolic and genomic traits associated with XOS metabolism in YT108 and to assess its probiotic attributes through whole genome sequencing and in vitro assays. Strain YT108 exhibited robust growth kinetics on XOS as the sole carbon source, with a growth profile comparable to that on glucose, achieving a pH reduction to 4.68 and an OD600nm of 1.603 after 48 h. Three key gene clusters (xylCDEPFRT, xylHTG, and xylABT) and key enzymes (1,4-β-xylosidase) were identified as potentially involved in XOS metabolism. In vitro assays confirmed the strain's remarkable physiological properties, including tolerance to acid, bile, heat, and NaCl, as well as resistance to simulated gastrointestinal juices and antioxidant capacity. Furthermore, strain YT108 was sensitive to five commonly used antibiotics and lacked transferable resistance genes. Taken together, these results highlight the potential of L. brevis YT108 as a probiotic candidate with beneficial traits for XOS utilization, suggesting its promising application in the formulation of next-generation synbiotic products.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and antimicrobial resistance profiles of Salmonella enterica recovered from house fly intestinal tracts and environments of selected broiler farms in Texas.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf007

Kirsten M Zwally, Elizabeth Holda, Isaac Perez, Phillip E Kaufman, Brandon Lyons, Giridhar Athrey, T Matthew Taylor

{"title":"Detection and antimicrobial resistance profiles of Salmonella enterica recovered from house fly intestinal tracts and environments of selected broiler farms in Texas.","authors":"Kirsten M Zwally, Elizabeth Holda, Isaac Perez, Phillip E Kaufman, Brandon Lyons, Giridhar Athrey, T Matthew Taylor","doi":"10.1093/lambio/ovaf007","DOIUrl":"10.1093/lambio/ovaf007","url":null,"abstract":"The entry of drug-resistant Salmonella enterica into the food supply is a challenge to public health and food safety. One emerging concern is the role of synanthropic insects for moving microbial pathogens throughout poultry production systems, where insects commonly thrive. We investigated the presence and phenotypic antimicrobial susceptibility of S. enterica from insect and environmental samples from broiler farms. Insects were collected throughout the broiler house and adjacent compost barn. Environmental samples (poultry feed, drinking water, fresh litter, and feces) were collected simultaneously (n = 80). Insect gastrointestinal tracts were dissected and pooled (n = 57). Recovered Salmonella isolates were serotyped and subjected to antimicrobial susceptibility testing against 14 medically important antimicrobials. Overall, six isolates were recovered from 137 total samples (4.4%): 3.5% (2/57) from adult house flies (Musca domestica), 15% (3/20) from poultry feed, and 4.8% (1/21) from litter. Salmonella Montevideo (16.7%; 1/6), Typhimurium (33.3%; 2/6), and Kentucky (50%, 3/6) were identified. All but one Salmonella isolate (83.3%; 5/6) demonstrated resistance to at least one antimicrobial. Further research should investigate movement patterns between broiler operations and food processing facilities to establish efficient biosecurity measures to prevent any instances of foodborne pathogen transmission into human food systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common mycorrhizal networks enhance growth and nutrient uptake in non-mycorrhizal Chenopodium album via Parthenium hysterophorus. 普通菌根网络通过帕特嫩草促进非菌根茄子的生长和营养吸收。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf009

Ambika Chauhan, Mankeet Kaur, Meenakshi Singh, Pradeep Kumar Singh

{"title":"Common mycorrhizal networks enhance growth and nutrient uptake in non-mycorrhizal Chenopodium album via Parthenium hysterophorus.","authors":"Ambika Chauhan, Mankeet Kaur, Meenakshi Singh, Pradeep Kumar Singh","doi":"10.1093/lambio/ovaf009","DOIUrl":"10.1093/lambio/ovaf009","url":null,"abstract":"Common mycorrhizal networks (CMNs) facilitate nutrient transfer between plants, but their role in supporting non-mycorrhizal species remains largely unexplored. This study investigates the effect of CMNs on the growth and nutrient uptake of the non-mycorrhizal plant Chenopodium album in association with the mycorrhizal plant Parthenium hysterophorus. The treatments included C. album alone, C. album grown with P. hysterophorus to allow CMN formation, and C. album treated with fungicide to inhibit mycorrhizal activity. Results showed that CMN treatment significantly enhanced the plant growth and uptake of nutrient content (N and P) in C. album compared to the control. There was no mycorrhizal colonization in C. album, while high colonization in P. hysterophorus suggested that the enhanced growth in C. album was due to nutrient uptake transfer through the CMNs. The fungicide treatment resulted in reduced growth and uptake of nutrient content, providing further evidence that CMNs and mycorrhizal associations of mycorrhizal plants enhanced the growth of C. album. These findings provide the first evidence that CMNs can enhance the growth and nutrient uptake of non-mycorrhizal plants through associations with mycorrhizal partners.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the bacterial communities in the sediment-water interface across different salinities of Pacific White shrimp, Penaeus vannamei, by metabarcoding.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf020

Natarajan Lalitha, Vinaya Kumar Katneni, Ashok Kumar Jangam, Panjan Nathamuni Suganya, Suvana Sukumaran, Moturi Muralidhar

{"title":"Insight into the bacterial communities in the sediment-water interface across different salinities of Pacific White shrimp, Penaeus vannamei, by metabarcoding.","authors":"Natarajan Lalitha, Vinaya Kumar Katneni, Ashok Kumar Jangam, Panjan Nathamuni Suganya, Suvana Sukumaran, Moturi Muralidhar","doi":"10.1093/lambio/ovaf020","DOIUrl":"10.1093/lambio/ovaf020","url":null,"abstract":"Microbes play an important role in the food chain by metabolizing organic matter, cycling nutrients, and maintaining a dynamic equilibrium among organisms in water and sediment. The objective is to study the fluctuating taxonomic microbial diversity profile in the sediment-water interface at different days of culture (DOC) of Penaeus vannamei in varying salinities using the Illumina MiSeq platform. Sediment samples were collected in Tamil Nadu, India, from low-saline, brackish water, and high-saline ponds at 30, 60, and 90 DOC. Bacterial richness and diversity in species were high in low-saline ponds. Beta-diversity variation indicated more differences in bacterial composition in high- and low-saline ponds. The predominant phyla observed were Proteobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, Firmicutes, and Cyanobacteria. High-saline ponds accounted for more nitrification bacterial communities, sulfur-reducing bacterial communities, sulfur-oxidizing bacterial communities, and high redox potential, whereas denitrification bacterial communities were high in brackish water ponds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the preparation of a spiny spore high-concentrated Aspergillus brasiliensis suspension. 刺孢子高浓度巴西曲霉悬浮液制备工艺优化。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae141

Federica Pisa, Mirella Gionta, Antonio Biasi, Maura Pizzuti, Jodi R Alt, Imma Zampaglione

{"title":"Optimization of the preparation of a spiny spore high-concentrated Aspergillus brasiliensis suspension.","authors":"Federica Pisa, Mirella Gionta, Antonio Biasi, Maura Pizzuti, Jodi R Alt, Imma Zampaglione","doi":"10.1093/lambio/ovae141","DOIUrl":"10.1093/lambio/ovae141","url":null,"abstract":"This work aimed to improve some steps of the existing guidelines of the European Standards to obtain an Aspergillus brasiliensis ATCC 16404 spore suspension with >75% spiny spores without mycelia and a concentration of at least 1.5 × 108 CFU ml-1. Several manufacturers' combinations of \"strain/medium\" were assessed in terms of yield of spiny spores. Criteria to establish success included (i) the growth over different times of incubation (4, 7, and 10 days); (ii) the performance of two different filtration devices (fritted filters and cell strainers); and (iii) the fungal spores susceptibility after 4 days of incubation versus 7 days of incubation against a peracetic acid-based disinfectant. The best combination was \"Microbiologics strain/Oxoid MEA\" with 88.33% of spiny spores already after 4 days, and no statistical differences (P < .05) in terms of growth in the three time points were highlighted (from 1.85 × 108 CFU ml-1 to 2.21 × 108 CFU ml-1). Cell strainers gave a spore suspension much more concentrated (3.31 times) compared to fritted filters. Furthermore, the 4-day-old spore suspension showed the same susceptibility to disinfection when compared to a suspension whose culture was incubated for 7 days.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of root-lesion nematode, Pratylenchus species, and their prevalence in New Zealand maize fields. 新西兰玉米根损线虫的分子特征及其流行状况。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae140

Nagarathnam Thiruchchelvan, Manjula Kularathna, Romy Moukarzel, Seona Casonato, Leo M Condron

{"title":"Molecular characterization of root-lesion nematode, Pratylenchus species, and their prevalence in New Zealand maize fields.","authors":"Nagarathnam Thiruchchelvan, Manjula Kularathna, Romy Moukarzel, Seona Casonato, Leo M Condron","doi":"10.1093/lambio/ovae140","DOIUrl":"10.1093/lambio/ovae140","url":null,"abstract":"Root-lesion nematodes (Pratylenchus spp.) are significant plant parasites, causing substantial crop damage worldwide. This study aimed to characterize Pratylenchus spp. in New Zealand maize fields using molecular techniques and map their prevalence. Soil sampling from 24 maize fields across the North and South Islands provided 381 composite samples. Root-lesion nematodes were extracted using the sieving-centrifugal-sugar flotation method and differentiated into five morphospecies. Molecular characterization involved direct partial sequencing of the D2/D3 28S rDNA, ITS rDNA, and COX1 mtDNA regions using Sanger technology from a single nematode. Five Pratylenchus species were identified: P. neglectus, P. crenatus, P. thornei, P. penetrans, and P. pratensis, confirmed by phylogenetic analysis. Prevalence mapping showed P. neglectus and P. crenatus in all sampled fields, while P. thornei, P. penetrans, and P. pratensis were more localized. This study is the first to report these Pratylenchus species on maize in New Zealand and provides the first partial sequences of the D2/D3, COX1, and ITS regions for these species on maize in New Zealand. The findings highlight the diversity of Pratylenchus populations in New Zealand maize fields and emphasize the need for region-specific management strategies to mitigate crop damage.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting. 利用移动实验室检测和定量废水中的 SARS-CoV-2

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-01-06 DOI: 10.1093/lambio/ovae135

Shubham Mathur, Ikram Salah, Haneen Msarwa, Hillary A Craddock, Jacob Moran-Gilad

{"title":"Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting.","authors":"Shubham Mathur, Ikram Salah, Haneen Msarwa, Hillary A Craddock, Jacob Moran-Gilad","doi":"10.1093/lambio/ovae135","DOIUrl":"10.1093/lambio/ovae135","url":null,"abstract":"This study evaluated a point-of-care device for wastewater-based epidemiology (WBE) of SARS-CoV-2 circulation. WBE is a critical tool but faces challenges such as laboratory infrastructure, sample transport, skilled personnel, and time-consuming methods. Currently, a rapid, portable solution for wastewater surveillance is lacking. From June to September 2022, the performance of the GeneXpert instrument was compared with an established in-house method for detecting SARS-CoV-2 in 151 wastewater samples across laboratory, field, and mobile testing environments. In the laboratory setting, GeneXpert showed 100% agreement in detection, with 94.6% of positive samples displaying a viral load difference of ± 1 log compared to the in-house method. Field testing demonstrated 90% agreement, with 92.6% of GeneXpert-positive samples showing similar log differences. In mobile testing, GeneXpert detected 62.5% of samples, and testing faced technical issues. Overall, GeneXpert displayed a limit of detection of 12 cpml-1 with results available in under an hour, making it a promising tool for rapid and deployable wastewater testing. This requires further validation while addressing technical challenges related to field and mobile settings.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0