Letters in Applied Microbiology最新文献_第4页

An enhanced model for accumulated CO2 concentration in closed Escherichia coli fermenters. 封闭大肠杆菌发酵罐中积累CO2浓度的增强模型。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf052

Yiqin Dai, Wen Liu, Xiaowei Dong, Qi Li

引用次数: 0

Bacterial consortia of ewes' whey in the production of bryndza cheese in Slovakia. 斯洛伐克bryndza奶酪生产中母羊乳清的细菌联合体。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf047

Nikola Klištincová, Janka Koreňová, Zuzana Rešková, Zuzana Čaplová, Alexandra Burdová, Zuzana Farkas, Martin Polovka, Hana Drahovská, Domenico Pangallo, Tomáš Kuchta

{"title":"Bacterial consortia of ewes' whey in the production of bryndza cheese in Slovakia.","authors":"Nikola Klištincová, Janka Koreňová, Zuzana Rešková, Zuzana Čaplová, Alexandra Burdová, Zuzana Farkas, Martin Polovka, Hana Drahovská, Domenico Pangallo, Tomáš Kuchta","doi":"10.1093/lambio/ovaf047","DOIUrl":"10.1093/lambio/ovaf047","url":null,"abstract":"Whey from previous production is often used as a natural starter in the technology of traditional cheeses, including bryndza cheese in Slovakia. Therefore, studying its bacterial community and isolating new potential natural starters is important for improving the characteristics of the final product. Composition of bacterial consortia of fresh and fermented whey in the production of raw ewes' milk-based bryndza cheese from 8 small or medium-sized producers was analysed. Culture-based microbiological analysis and culture-independent analysis based on 16S rRNA gene sequencing by MiSeq and MinION were used. Results showed the dominance of lactococci or streptococci, with 3-8 log CFU ml-¹ of Lactobacillus sensu lato in all whey samples. Potential natural starters comprising Lacticaseibacillus paracasei/casei, Lactiplantibacillus plantarum, Lentilactobacillus parabuchneri, Lactobacillus helveticus, L. diolivorans, Levilactobacillus brevis, Limosilactobacillus fermentum, L. delbrueckii, L. gasseri and L. otakiensis were isolated. Coliforms were also present in all samples, with no consistently lower values in fermented whey samples. Some samples contained pseudomonads and/or acinetobacters. Coagulase-positive staphylococci were present at relevant levels in samples from 4 producers. The results revealed that whey is a source of natural starters due to the presence of lactobacilli.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promising antifungal properties of the orally active autophagy inhibitor SBP-7455 against fluconazole-resistant Candida clinical isolates. 口服活性自噬抑制剂SBP-7455对氟康唑耐药念珠菌临床分离株具有良好的抗真菌性能。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf055

Mark A Rassan, Radwa Ewaisha, Hend Zeitoun, Michael G Shehat

{"title":"Promising antifungal properties of the orally active autophagy inhibitor SBP-7455 against fluconazole-resistant Candida clinical isolates.","authors":"Mark A Rassan, Radwa Ewaisha, Hend Zeitoun, Michael G Shehat","doi":"10.1093/lambio/ovaf055","DOIUrl":"https://doi.org/10.1093/lambio/ovaf055","url":null,"abstract":"Candida species, the single most common cause of fungal infections, are major opportunistic pathogens. Novel antifungal agents are needed to address the threat of Candida infections resistant to first-line antifungal agents and those that are multi-drug resistant, both being increasingly reported. Here, we explore the antifungal properties of the novel autophagy inhibitor SBP-7455, whose anticancer effects have been recently described. Using broth microdilution, SBP-7455 inhibited the fluconazole-resistant standard C. albicans strain with minimum inhibitory concentration (MIC) values of 43.91 and 21.95 µM in the presence and absence of d-glucose, respectively. SBP-7455 inhibited the growth of six fluconazole-resistant Candida clinical isolates (MIC range 5.48-87.82 µM). Using the checkerboard assay, the fluconazole-resistant standard strain (MIC > 250 µg/ml) was rendered sensitive (MIC = 3.9 µg/ml) to fluconazole when combined with SBP-7455, but combining SBP-7455 with chloroquine was antagonistic. Compared with control, SBP-7455 treated cell membranes showed disrupted integrity and bulging on SEM images. Treatment with SBP-7455 significantly (P < 0.01) increased reduced glutathione levels with no significant change in nitric oxide levels, possibly adapting to oxidative stress induced by autophagy inhibition. Taken together, our results report for the first time the promising antifungal effects of the dual autophagy inhibitor SBP-7455 against fluconazole-resistant Candida, worthy of further investigation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of commercial sanitizers on Listeria monocytogenes (planktonic and biofilm forms) experimentally inoculated materials commonly used during tree-fruit harvesting. 商业杀菌剂对果树采收过程中常用的单核细胞增生李斯特菌（浮游和生物膜形式）实验接种材料的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf056

Savannah Stewart, Samhitha Chalamalasetti, Blanca Ruiz-Llacsahuanga, Faith Critzer, Manreet Bhullar, Londa Nwadike, Umut Yucel, Valentina Trinetta

{"title":"The effect of commercial sanitizers on Listeria monocytogenes (planktonic and biofilm forms) experimentally inoculated materials commonly used during tree-fruit harvesting.","authors":"Savannah Stewart, Samhitha Chalamalasetti, Blanca Ruiz-Llacsahuanga, Faith Critzer, Manreet Bhullar, Londa Nwadike, Umut Yucel, Valentina Trinetta","doi":"10.1093/lambio/ovaf056","DOIUrl":"https://doi.org/10.1093/lambio/ovaf056","url":null,"abstract":"This study compares the efficacy of commercially available sanitizers in reducing Listeria monocytogenes planktonic and biofilm form on surfaces commonly used during tree fruit harvesting. Planktonic L. monocytogenes cells were grown to create a bacterial lawn and inoculated on high-density polyethylene plastic, wood, or nylon. Biofilms were grown for 96 h in a Centers for Disease Control reactor on the same surfaces. Bacteria were exposed to free chlorine, peroxyacetic acid, silver-dihydrogen citrate, steam, and chlorine dioxide. In planktonic forms, peroxyacetic acid and silver-dihydrogen citrate, applied for 2 min resulted in ≥3-log reduction regardless surface type (P < 0.05). Steam and chlorine dioxide efficacy differed significantly by surface type. When applied to Listeria biofilms, steam, chlorine, peroxyacetic acid, and chlorine dioxide resulted in an overall significant difference compared to untreated coupons for all surfaces (P < 0.05). Silver-dihydrogen citrate significantly affected population on plastic (P < 0.05), but not on wood or nylon. For porous surfaces few treatments, such as peroxyacetic acid (for nylon) and chlorine dioxide (for wood), resulted in a ≥3 log reduction of both forms. Interactions between sanitizer, surfaces, and exposure time should be considered by tree fruit harvesting facilities when determining sanitation strategies to be implemented.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-free supernatant of Clostridium leptum inhibits breast cancer cell proliferation. 瘦梭菌无细胞上清液抑制乳腺癌细胞增殖。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf037

Qingling Wang, Huan Zhao, Huizhe Ding, Hao Zhang, Jizhou Zhang, Liqin Li, Bing Han, Guoyin Kai

{"title":"Cell-free supernatant of Clostridium leptum inhibits breast cancer cell proliferation.","authors":"Qingling Wang, Huan Zhao, Huizhe Ding, Hao Zhang, Jizhou Zhang, Liqin Li, Bing Han, Guoyin Kai","doi":"10.1093/lambio/ovaf037","DOIUrl":"10.1093/lambio/ovaf037","url":null,"abstract":"Breast cancer has emerged as the leading cause of global cancer incidence, surpassing lung cancer. Accumulating evidence suggests that probiotics exhibit inhibitory effect on breast cancer progression, highlighting the need to identify gut flora-derived probiotics with potential anti-breast cancer properties. Here, we investigated the effect of the cell-free supernatant of Clostridium leptum (ClCFS) on breast cancer cells by methyl thiazolyl tetrazolium (MTT) assay. Untargeted metabolomics analysis was employed to characterize metabolite alterations in ClCFS. Furthermore, the core targets were predicted by the protein-protein interaction network and the signaling pathways were enriched by the Kyoto Encyclopedia of Genes and Genomes analysis. Our findings demonstrated that ClCFS inhibited the proliferation of breast cancer cells and that the main metabolite of ClCFS might be acetylcarnitine. Utilizing network pharmacological analysis, we identified apoptosis-related signaling pathways as the principal mechanisms underlying ClCFS activity. Furthermore, five core targets of STAT3, IL-1β, BCL2, CASP3, and ESR1 were identified. This study elucidates the main bioactive constituent and the potential targets of ClCFS against breast cancer. It provides a new understanding of the pharmacological activity of ClCFS in breast cancer treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of fermentation conditions for enhancing the antioxidant activity of medicine and food homologous materials using Lactobacillus plantarum. 植物乳杆菌提高药物和食品同源材料抗氧化活性的发酵条件优化。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf051

Yikai Wang, Qiming Chen, Zhengrong Lu, Quanling Dong, Jiaxu Wang, Yuanlong Hu, Tiantian Tang, Zhanmin Liu

{"title":"Optimization of fermentation conditions for enhancing the antioxidant activity of medicine and food homologous materials using Lactobacillus plantarum.","authors":"Yikai Wang, Qiming Chen, Zhengrong Lu, Quanling Dong, Jiaxu Wang, Yuanlong Hu, Tiantian Tang, Zhanmin Liu","doi":"10.1093/lambio/ovaf051","DOIUrl":"10.1093/lambio/ovaf051","url":null,"abstract":"Codonopsis radix, Astragalus membranaceus, Platycodon grandiflorus, and Tiger milk mushroom are promising candidates for functional food development. However, their limited bioavailability impedes a challenge to broader applications. In this study, fermentation of these medicine and food homologous (MFH) materials using Lactobacillus plantarum was optimized to enhance antioxidant activity. Through single-factor experiments and Pareto analysis, the key factors influencing DPPH radical scavenging activity, including the material-to-water ratio, sucrose concentration, and inoculum size, were identified. Response surface methodology and artificial neural networks were then employed to optimize fermentation parameters. The optimal conditions resulted in a DPPH radical scavenging rate of 95.8%. Furthermore, the levels of bioactive compounds, including polysaccharides, polyphenols, flavonoids, and saponins, were significantly elevated, which enhanced antioxidant activity and may have improved bioavailability. FTIR analysis confirmed the structural changes in the active compounds after fermentation. This study provides valuable insights into developing MFH-based probiotic fermented products, enhancing their nutritional and biological properties.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of multiplex PCR for detection of foodborne pathogens in fresh produce. 多重PCR检测新鲜农产品食源性致病菌的研究进展。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf038

Ian Moppert, Eiseul Kim, Seung-Min Yang, Si Hong Park

{"title":"Development of multiplex PCR for detection of foodborne pathogens in fresh produce.","authors":"Ian Moppert, Eiseul Kim, Seung-Min Yang, Si Hong Park","doi":"10.1093/lambio/ovaf038","DOIUrl":"10.1093/lambio/ovaf038","url":null,"abstract":"Foodborne pathogens present a significant public health concern where fresh produce is a key agricultural product. Rapid and sensitive detection methods are essential to ensure the safety of such produce. This study aimed to develop and optimize a modified multiplex polymerase chain reaction (mPCR) assay, which incorporates enhancements to conventional PCR, for the simultaneous detection of Escherichia coli, Salmonella, and Listeria monocytogenes in fresh produce. The specificity of each primer pair was validated using 15 strains, confirming 100% accurate detection of pathogenic strains without cross-reactivity. Since no false positives were observed, the assay demonstrated 100% precision, highlighting its reliability in distinguishing target pathogens. The sensitivity of the mPCR assay was demonstrated through serial dilutions, detecting Salmonella down to 10 fg µl-1, L. monocytogenes to 100 fg µl-1, and E. coli to 1 pg µl-1. The mPCR assay was then successfully applied to romaine lettuce and kale, demonstrating its effectiveness in detecting pathogens in mixed samples inoculated at varying concentrations (109-101 CFU ml-1). Kale exhibited greater sensitivity, detecting pathogens at lower levels, while romaine lettuce also provided consistent detection. This study highlights the potential of mPCR for enhancing food safety by providing rapid and sensitive pathogen detection in fresh produce.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lead (Pb) removal and suppression of Pb-induced inflammation by Lacticaseibacillus rhamnosus A6-6. 鼠李糖乳杆菌A6-6去除铅及抑制铅诱导炎症的研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf039

Se-Won Lee, Jeong-Muk Lim, Hyeon-Jin Jeong, Gwang-Min Lee, Kamala-Kannan Seralathan, Jung-Hee Park, Byung-Taek Oh

引用次数: 0

Whole genome sequencing and phylogenetic relative of a pure virulent Newcastle disease virus isolated from an outbreak in northeast Iran. 从伊朗东北部一次疫情中分离出的一种纯新城疫病毒的全基因组测序和系统发育亲缘关系。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf049

Aliakbar Khabiri, Reza Toroghi, Mohammadreza Mohammadabadi, Seyed-Elias Tabatabaeizadeh

{"title":"Whole genome sequencing and phylogenetic relative of a pure virulent Newcastle disease virus isolated from an outbreak in northeast Iran.","authors":"Aliakbar Khabiri, Reza Toroghi, Mohammadreza Mohammadabadi, Seyed-Elias Tabatabaeizadeh","doi":"10.1093/lambio/ovaf049","DOIUrl":"10.1093/lambio/ovaf049","url":null,"abstract":"Newcastle disease (ND) is a significant viral disease affecting poultry worldwide, with outbreaks persisting despite extensive vaccination efforts. This study characterizes a novel NDV strain, RT40, isolated from poultry farms in northeast Iran for the first time. RT40, classified as genotype VII, subgenotype VII.1.1, has a genome length of 15 192 nucleotides, with six genes, 12 UTRs, and five IGS, consistent with class II NDVs. Pathogenicity tests confirm its virulent velogenic nature, differentiating it from other known strains. Phylogenetic analysis points to a Western origin, suggesting possible cross-border transmission with Iraqi strains, highlighting the strain's relevance for NDV surveillance. Virus isolation, RNA sequencing, and cross-border transmission analysis were performed, revealing that RT40 shares a high nucleotide sequence identity with Iraqi strains, further supporting cross-border transmission concerns. The findings underscore the need for genotype-specific vaccines tailored to circulating strains. The genome, deposited in GenBank (accession ON184061), serves as an essential resource for future research and vaccine development. By emphasizing whole-genome sequencing, this study aims to improve NDV evolution tracking and enhance understanding of NDV diversity. This work calls for targeted vaccine strategies to effectively manage NDV spread, ensuring the sustainability of the poultry industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization, antifungal activity, and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains. 菝葜精油对假丝酵母菌和新型隐球菌的抑菌活性及其可能的作用机制。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf040

Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior

{"title":"Characterization, antifungal activity, and possible action mechanism of Melissa officinalis essential oil against Candida spp. and Cryptococcus neoformans strains.","authors":"Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Ingrid de Carvalho Follone, Álvaro Gabriel de Souza Araújo, Marcos Vinicius Queiroz Ambrósio Correia, Daniel Sampaio Rodrigues, Vitória Pessoa de Farias Cabral, Lara Elloyse Almeida Moreira, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Cecília Rocha da Silva, Manoel Odorico de Moraes, Cláudio Costa Dos Santos, Hélio Vitoriano Nobre Júnior","doi":"10.1093/lambio/ovaf040","DOIUrl":"10.1093/lambio/ovaf040","url":null,"abstract":"This study aimed to characterize Melissa officinalis essential oil (EOMO) from a region in Northeast Brazil and evaluate its antifungal activity against Candida and Cryptococcus neoformans, by analyzing its action mechanism. EOMO was characterized using gas chromatography-mass spectrometry and the main chemical compounds were Geranial (14.10%) and (Z)-Nerolidol (17.75%). The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) against strains of Candida albicans, Candida parapsilosis, Candida krusei, Candida auris (MIC raging from 256 to 26.7 µg ml-1) and C. neoformans (MIC ranging from 64 to 32 µg ml-1). Flow cytometry and comet assays were employed to investigate EOMO's mechanism of action, which might be related to an increase in the production of reactive oxygen species and damage to fungal DNA. The chemical composition of EOMO from Northeast Brazil showed a higher content of (Z)-Nerolidol and has significant antifungal potential.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0