Letters in Applied Microbiology最新文献_第4页

Selection of resistant coliform bacteria in the intestine of pigs following flock versus individual treatment with neomycin against post-weaning diarrhoae or amoxicillin against umbilical infection.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf008

Egle Kudirkiene, Esben Østergaard Eriksen, Gang Liu, Anja Ejlersgård Christensen, Marianne Viuf Agerlin, Inge Larsen, Nicolai Rosager Weber, Ken Steen Pedersen, Jens Peter Nielsen, John Elmerdahl Olsen

{"title":"Selection of resistant coliform bacteria in the intestine of pigs following flock versus individual treatment with neomycin against post-weaning diarrhoae or amoxicillin against umbilical infection.","authors":"Egle Kudirkiene, Esben Østergaard Eriksen, Gang Liu, Anja Ejlersgård Christensen, Marianne Viuf Agerlin, Inge Larsen, Nicolai Rosager Weber, Ken Steen Pedersen, Jens Peter Nielsen, John Elmerdahl Olsen","doi":"10.1093/lambio/ovaf008","DOIUrl":"10.1093/lambio/ovaf008","url":null,"abstract":"The aim of this study was to investigate the effect of avoiding flock treatment on resistance levels in the intestine of pigs. To investigate this, studies, each in two pig herds, quantified resistant coliforms by culture method in pigs treated as flock or as individual animal treatments orally with neomycin against post-weaning diarrhea (PWD) or intramuscularly with amoxicillin to prevent umbilical infection. Individual oral treatment against PWD did not lead to a lower number or proportion of resistant coliforms compared to flock treated pigs in any of the two herds under study, despite reduction of treatment incidences to 32% and 35% of pigs. After intramuscular treatment against umbilical infection, fewer resistant bacteria were seen in individually treated piglets in a herd with low treatment incidence (33%), while no reduction of resistant coliform bacteria compared to flock treatment was observed in the other herd with higher treatment incidence (86%). Thus, individual animal treatment reduced the amount of antimicrobial used, but concurrent reduction in resistant coliform bacteria was not always observed.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Enrichment of laccase production by Phoma herbarum isolate KU4 under solid-state fermentation by optimizing RSM coefficients using genetic algorithm.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf018

引用次数: 0

The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf012

Yuchun Liu, Rui Zhu, Dongmei Liu, Yue Hu, Qing Xia, Xinwei Liu, Chunxia Wang, Yongwei Li

{"title":"The characterization of Herbaspirillum huttiense isolated from a uremic patient: virulence and antimicrobial efficacy in the Galleria mellonella model.","authors":"Yuchun Liu, Rui Zhu, Dongmei Liu, Yue Hu, Qing Xia, Xinwei Liu, Chunxia Wang, Yongwei Li","doi":"10.1093/lambio/ovaf012","DOIUrl":"10.1093/lambio/ovaf012","url":null,"abstract":"Herbaspirillum species are typically isolated from plants and are known for their role in crop nitrogen fixation. Recently, they have been found to colonize humans and cause infections. This study aimed to identify and characterize two Herbaspirillum huttiense strains (CLJ01 and CLJ02) isolated from the blood of a uremic patient, with a focus on evaluating their pathogenicity and antibiotic efficacy. The strains were identified using the VITEK2 system, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry , and 16S rRNA gene sequencing, confirming their identity as H. huttiense. High-throughput sequencing further revealed the presence of the resistance gene arnA, which confers resistance to polymyxin. For the first time, the pathogenicity of H. huttiense was assessed using a Galleria mellonella infection model. The results demonstrated a concentration-dependent virulence, with CLJ01 exhibiting slightly higher pathogenicity. Additionally, meropenem showed significant antimicrobial efficacy in the G. mellonella infection model, particularly under conditions of high bacterial load, indicating strong therapeutic potential. In conclusion, this study provides experimental evidence supporting the correct diagnosis and treatment of H. huttiense infections. Furthermore, the findings underscore the importance of accurately identifying rare pathogens in clinical settings for effective treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf013

Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling

{"title":"Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater.","authors":"Lynette G Balisacan, Prima Fe R Franco, Henedine A Aguinaldo, Mae Ann R Batuyong, Alice Geraldine Hernando-Pagaling","doi":"10.1093/lambio/ovaf013","DOIUrl":"10.1093/lambio/ovaf013","url":null,"abstract":"Sumiling Dam in Sarrat, Ilocos Norte, is home to diverse phytoplankton species. This study aims to isolate and identify phytoplankton species, optimize the cultivation of one phytoplankton under varying light, pH, and temperature conditions, and assess its bioremediation capacity in aquaculture wastewater. Three stations were set up at the site, each with three sampling points spaced 3-5 m apart. From each point, 300 ml of water was collected using standard procedures and transported to the lab for phytoplankton identification. Samples were incubated in algae culture broth for 14 days, streak-plated for isolation, and cultured under light. Microscope examination was used to characterize phytoplankton species, and the identification was verified by a professional. Ten phytoplankton species were identified, with Haematococcus sp. selected for wastewater bioremediation due to its optimal growth under specific conditions (81 µmol m-2 s-1, pH 9, 25°C ± 2°C). Experiments showed that Haematococcus sp. significantly reduced nitrogen and phosphorus in wastewater over 7-14 days, with Treatment 3 (1000 mg/l) achieving the highest growth, chlorophyll, and nitrogen reduction, while Treatment 2 (750 mg/l) excelled in phosphorus removal. These results underscore phytoplankton's potential for sustainable, large-scale wastewater bioremediation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between pathogenic bacteria and different stages of colorectal cancer.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf017

Javad Nezhadi, Hossein Samadi Kafil, Mohammad Sadrkabir, Farshad Mahdavi, Seyed Yaghoub Moaddab, Roghayeh Nouri, Yalda Mohammadzadeh-Asl, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee

{"title":"The relationship between pathogenic bacteria and different stages of colorectal cancer.","authors":"Javad Nezhadi, Hossein Samadi Kafil, Mohammad Sadrkabir, Farshad Mahdavi, Seyed Yaghoub Moaddab, Roghayeh Nouri, Yalda Mohammadzadeh-Asl, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee","doi":"10.1093/lambio/ovaf017","DOIUrl":"10.1093/lambio/ovaf017","url":null,"abstract":"Colorectal cancer (CRC) involves uncontrolled cell growth in the colon and rectum. This study aims to explore the prevalence of key pathogenic bacteria and their role in the progression of CRC, focusing on microbial dysbiosis. This study analyzed 52 stool and tissue samples through polymerase chain reaction (PCR), real-time PCR, and bioinformatics to identify associations between pathogenic bacteria and CRC progression. PCR results revealed a significant association between the Bacteroides fragilis toxin (bft) gene and CRC progression (P = 0.001, r = 0.570). Furthermore, Real-time PCR showed significant differences in the frequency of pks+Escherichia coli in CRC stages 1 (P = 0.03), 2 (P = 0.004), and 3 (P = 0.0002) compared to the control group. Additionally, the frequency of Fusobacterium nucleatum in stage 3 CRC patients was significantly higher than in the control group (P = 0.004) and stage 1 patients (P = 0.01). Furthermore, Streptococcus gallolyticus showed similar significant differences in stage 3 patients (P = 0.004). Bioinformatics analyses using KEGG, Reactome, STRING, and dbSNP highlighted bacteria's roles in colorectal carcinogenesis, emphasizing the need for early identification and management in CRC treatment and prevention strategies. Finally, due to the limitations of the study, the use of more advanced methods and the validation of results through more reliable techniques are essential for future research.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a quantitative SYBR Green real-time PCR and microscopic slide culture for in vitro enumeration of Mycoplasma hyopneumoniae.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf011

Raziallah Jafari Jozani, Mauida F Hasoon Al Khallawi, Majed H Mohammed, Hanh Thi Hong Nguyen, Darren J Trott, Kiro Petrovski, Farhid Hemmatzadeh

{"title":"Development of a quantitative SYBR Green real-time PCR and microscopic slide culture for in vitro enumeration of Mycoplasma hyopneumoniae.","authors":"Raziallah Jafari Jozani, Mauida F Hasoon Al Khallawi, Majed H Mohammed, Hanh Thi Hong Nguyen, Darren J Trott, Kiro Petrovski, Farhid Hemmatzadeh","doi":"10.1093/lambio/ovaf011","DOIUrl":"https://doi.org/10.1093/lambio/ovaf011","url":null,"abstract":"Mycoplasma hyopneumoniae's slow growth makes enumeration challenging using the traditional colony-forming unit (CFU) method. We introduced an innovative CFU-slide culture (CFU-SC) approach to address this issue. CFU-SC allows accurate counting of M. hyopneumoniae microcolonies, ranging from 7 to 50 µm, using high magnification. While comparing the accuracy of enumeration among CFU, CFU-SC, quantitative polymerase chain reaction (qPCR), and colour change unit (CCU), we found a robust positive correlation between qPCR and CFU-SC with colourCCU method for quantifying M. hyopneumoniae. Newly isolated strains exhibited the lowest Pearson correlation coefficient (r = 0.52) when comparing CFU and real-time PCR, while the highest coefficient (r = 0.86) was observed for the type strain J and new isolates using real-time PCR and CFU-SC. A consensus growth pattern was observed among all quantification methods, except CFU, between newly isolated strains and the type strain J. Notably, new isolates of M. hyopneumoniae showed no senescence phase after 228 h of cultivation, differing from the type strain J. The disparity in growth rate and pattern between new isolates and the type strain J is evident in the smaller agar microcolonies (7-10 µm) of the isolates, contrasting with the larger colonies (100-200 µm) of type strain J.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and anti-Xanthomonas citri activity of unguinol produced by Aspergillus unguis CBMAI 2140.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf016

Eduarda Araujo Dos Santos, Vítor Rodrigues Marin, Derlene Atilli de Angelis, Henrique Ferreira, Daiane Cristina Sass

{"title":"Isolation and anti-Xanthomonas citri activity of unguinol produced by Aspergillus unguis CBMAI 2140.","authors":"Eduarda Araujo Dos Santos, Vítor Rodrigues Marin, Derlene Atilli de Angelis, Henrique Ferreira, Daiane Cristina Sass","doi":"10.1093/lambio/ovaf016","DOIUrl":"10.1093/lambio/ovaf016","url":null,"abstract":"This research investigated active biomolecules from the endophytic fungus Aspergillus unguis (CBMAI 2140), isolated from Passiflora incarnata leaves, as potential antibacterial agents against Xanthomonas citri subsp. citri, the causative agent of citrus canker. The fungal extract was obtained via liquid-liquid extraction with ethyl acetate. Antibacterial activity was assessed using the Resazurin Microtiter Assay Plate, determining inhibitory concentrations (IC90) and the minimum bactericidal concentration (MBC). The extract displayed microbicidal activity at 1050 µg ml-¹ and inhibited bacterial growth at 350 µg ml-¹. Fractionation of the extract via normal-phase column chromatography yielded six fractions, with fraction F11 showing the highest antibacterial potential (MBC: 200 µg ml-¹). Nuclear magnetic resonance analysis identified Unguinol as the main compound in F11. Fluorescence microscopy demonstrated rapid permeabilization of the X. citri cell membrane upon exposure to the compound, with significant effects observed after 15-30 min. Although no notable anti-biofilm activity was detected, this study represents the first report of Unguinol's antibacterial activity against Xanthomonas species. These findings highlight its potential for agricultural applications, contributing to sustainable development goal 2.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urease-catalyzed microbial and enzymatic carbonate precipitation for eco-friendly heavy metal remediation.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf022

Adharsh Rajasekar, Armstrong Ighodalo Omoregie, Kan Fock Kui

{"title":"Urease-catalyzed microbial and enzymatic carbonate precipitation for eco-friendly heavy metal remediation.","authors":"Adharsh Rajasekar, Armstrong Ighodalo Omoregie, Kan Fock Kui","doi":"10.1093/lambio/ovaf022","DOIUrl":"10.1093/lambio/ovaf022","url":null,"abstract":"Heavy metal contamination significantly threatens environmental and public health, necessitating effective and sustainable remediation technologies. This review explores two innovative bioremediation techniques: microbially induced calcium carbonate precipitation (MICP) and enzyme-induced calcium carbonate precipitation (EICP). Both techniques show promise for immobilizing heavy metals in laboratory and field settings. MICP utilizes the metabolic activity of ureolytic microorganisms to precipitate calcium carbonate, sequestering heavy metals such as lead, cadmium, and arsenic as stable metal-carbonate complexes. EICP, on the other hand, employs urease enzymes to catalyze calcium carbonate precipitation, offering greater control over reaction conditions and higher efficiency in environments unfavorable to microbial activity. This mini-review compares the mechanisms of MICP and EICP, focusing on factors influencing their performance, including enzyme or microbial activity, pH, temperature, and nutrient availability. Case studies illustrate their success in sequestering heavy metals, emphasizing their practical applications and environmental benefits. A comparative analysis highlights the strengths and limitations of MICP and EICP regarding cost, scalability, and challenges. This review synthesizes research to support the advancement of MICP and EICP as sustainable solutions for mitigating heavy metal contamination.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and characterization of levans with different molecular weights synthesized by Bacillus licheniformis ZM107.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf015

Jingqi Yan, Xiangying Zhao, Liping Liu, Jiaxiang Zhang, Jing Ma

{"title":"Production and characterization of levans with different molecular weights synthesized by Bacillus licheniformis ZM107.","authors":"Jingqi Yan, Xiangying Zhao, Liping Liu, Jiaxiang Zhang, Jing Ma","doi":"10.1093/lambio/ovaf015","DOIUrl":"10.1093/lambio/ovaf015","url":null,"abstract":"Levan, a fructan-type polysaccharide with diverse applications in food, pharmaceuticals, and biotechnology, has garnered significant attention for its functional properties, such as prebiotic, immunomodulatory, antioxidant, and antimicrobial activities. In this study, the effects of fermentation temperature, sucrose concentration, and incubation time on the molecular weight of levan produced by Bacillus licheniformis fermentation were investigated. The results showed that as the temperature (37°C-50°C) and sucrose concentration (300-500 g l-1) increased, the molecular weight of levan produced by the strain during fermentation decreased. At the same time, the molecular weight initially increased and then decreased with longer culture times, indicating that levan within a specific size range could be produced by controlling the fermentation conditions. In addition, the properties and characteristics of levan produced by fermentation at two molecular weights (HML and LML) were compared. The results showed that molecular weight significantly affected the micromorphology, thermal behavior, rheological properties, and prebiotic activity of levan. Therefore, this study demonstrated that Bacillus licheniformis SFLV-ZM107 can produce low molecular weight levan, and its molecular weight can be controlled through fermentation conditions. Consequently, the properties and functions of levan can be influenced, allowing the production of levans with the desired characteristics.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-silico study of molecular adaptations in halophilic Cas9. 嗜盐Cas9分子适应性的计算机研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-02-03 DOI: 10.1093/lambio/ovaf006

Anisha Debnath, Aveepsa Sengupta, Sujata Rudrapal, Ashutosh Kumar, Mamta Rani

{"title":"In-silico study of molecular adaptations in halophilic Cas9.","authors":"Anisha Debnath, Aveepsa Sengupta, Sujata Rudrapal, Ashutosh Kumar, Mamta Rani","doi":"10.1093/lambio/ovaf006","DOIUrl":"10.1093/lambio/ovaf006","url":null,"abstract":"This study explores the structural adaptations of the CRISPR-Cas9 system in halophilic bacteria, focusing on Cas9 protein of halophilic bacterium Salicibibacter cibi. Protein sequences were analyzed using different tools such as ExPASy ProtParam for different physicochemical properties, Predictor of Natural Disordered Regions web server for disordered regions, and InterPro server and WebLogo for domains. Protein structures were generated using the AlphaFold database, and the quality of the modelled structure was checked through PROCHECK. The protein surface's amino acids and electrostatic potential were visualized using PyMOL, APBS server, and UCSF chimera. Comparative analysis revealed that halophilic Cas9 proteins possess a higher abundance of acidic residues, resulting in enhanced stability and hydration in saline conditions; halophilic Cas9 proteins also shows higher intrinsically disordered regions. Electrostatic potential maps confirmed that S. cibi Cas9 proteins maintain a highly negative surface charge, crucial for adaptation to salt-rich environments. These findings provide insights into the molecular mechanisms driving the structural and functional adaptations of Cas9 in salty environment, highlighting its potential applications in genome editing-based biotechnological approaches in extreme conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143007963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0