Letters in Applied Microbiology最新文献_第4页

Sublethal benzalkonium chloride exposure induced direct protection and cross-protection to freezing stress in Salmonella Typhimurium. 亚致死苯扎氯铵暴露诱导鼠伤寒沙门菌对冰冻胁迫的直接保护和交叉保护。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-08-03 DOI: 10.1093/lambio/ovag066

Yuan Liang, Xin Li, Changqian Zhou, Lu Sun

{"title":"Sublethal benzalkonium chloride exposure induced direct protection and cross-protection to freezing stress in Salmonella Typhimurium.","authors":"Yuan Liang, Xin Li, Changqian Zhou, Lu Sun","doi":"10.1093/lambio/ovag066","DOIUrl":"10.1093/lambio/ovag066","url":null,"abstract":"Benzalkonium chloride (BAC) is a widely used disinfectant, but its improper application can induce Salmonella Typhimurium (S. Typhimurium) to develop tolerance to subsequent stressors. In this study, we demonstrated that adaptation of S. Typhimurium to BAC conferred both direct protection and cross-protection against cold stress, resulting in significantly enhanced survival at -20°C compared with non-adapted cells. BAC-adapted S. Typhimurium also exhibited a survival advantage on chicken meat during frozen storage at -20°C. Membrane fatty acid analysis indicated that adaptation of S. Typhimurium to BAC led to a 2.2-fold increase in the levels of cyclopropane fatty acids. Moreover, BAC-adapted S. Typhimurium showed a significant 8.0% increase in cell surface hydrophobicity. RT-qPCR analysis demonstrated upregulation of efflux pump genes tolC, acrB, and mdfA (3.6-, 3.4-, and 3.0-fold, respectively) and cold shock-related genes cspA, cspE, and rpoS (2.4-, 4.0-, and 2.6-fold, respectively). These findings suggest that upregulation of cold shock genes in BAC-adapted cells was associated with the observed cross-protection. Taken together, adaptation of S. Typhimurium to BAC promoted the development of direct and cross-protective phenotypes, which may increase the risk of cross-contamination and salmonellosis in the food industry, posing a serious threat to food safety and public health.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mangrove health shapes lignocellulolytic bacterial communities. 红树林的健康状况塑造了木质纤维素分解细菌群落。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-07-03 DOI: 10.1093/lambio/ovag049

Rishi R Gandhi, Rakhee D S Khandeparker, P R Nikhita, Khanjan Chudasama

{"title":"Mangrove health shapes lignocellulolytic bacterial communities.","authors":"Rishi R Gandhi, Rakhee D S Khandeparker, P R Nikhita, Khanjan Chudasama","doi":"10.1093/lambio/ovag049","DOIUrl":"https://doi.org/10.1093/lambio/ovag049","url":null,"abstract":"The process of lignocellulosic biofuel production needs enzymes that are resistant to high temperatures and low pH. The mangrove sediments, which are typified by variable conditions, can contain bacteria that synthesize intrinsically steady enzymes. We selected 193 bacterial isolates of 12 mangrove sites in Goa, India and tested them to produce lignocellulolytic enzymes (cellulase, laccase, xylanase, xylose isomerase) under the conditions of neutral (37°C, pH 7), acidic (37°C, pH 5), thermophilic (50°C, pH 7), and combined stress (50°C, pH 5). Bacillus and Vibrio dominated, with 22 genera identified. There were no significant differences in alpha diversity following Benjamini-Hochberg FDR correction (all P_adj = 1.00, Cohen d < 0.5) but significant compositional differentiation in beta diversity (PERMANOVA: R² = 0.243, P = 0.017). Salinity (R² = 0.903, P_adj = 0.003) and temperature (R² = 0.722, P_adj = 0.006) were major structuring factors. Site-type differentiation was the most significant factor in xylanase-producing communities (R² = 0.272, P = 0.013). Although there was limited replication of dead sites (n = 3), the results confirmed that candidates undergo biochemical characterization and that ecosystem degradation does not decrease diversity but alters community composition.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"79 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cinnamon essential oil on quorum sensing-associated virulence and biofilm formation in Pseudomonas tolaasii. 肉桂精油对陶氏假单胞菌群体感应相关毒力和生物膜形成的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-07-03 DOI: 10.1093/lambio/ovag056

Xinyu Luan, Lisha Song, Xiuxiu Chen, Yan Feng, Huaiyuan Zhang, Xiangbo Han, Zhanli Liu

{"title":"Effects of cinnamon essential oil on quorum sensing-associated virulence and biofilm formation in Pseudomonas tolaasii.","authors":"Xinyu Luan, Lisha Song, Xiuxiu Chen, Yan Feng, Huaiyuan Zhang, Xiangbo Han, Zhanli Liu","doi":"10.1093/lambio/ovag056","DOIUrl":"10.1093/lambio/ovag056","url":null,"abstract":"Cinnamon essential oil (CEO), one of volatile plant compounds, exhibits potent inhibitory effects on bacterial growth. Pseudomonas tolaasii (P. tolaasii) is the primary spoilage bacterium that causes the decay of Agaricus bisporus. This study investigated the effects of CEO on quorum sensing (QS) and biofilm formation in Pseudomonas tolaasii. Whole-genome analysis revealed 102 QS-related genes and their association with 10 virulence protein clusters. AHL signaling molecules (C4-HSL, C6-HSL, C8-HSL, and 3-Oxo-C10-HSL) were identified using biosensor methods and LC-MS/MS. CEO significantly inhibited virulence factor secretion, including protease, elastase, and rhamnolipid. It also reduced swarming and swimming motility by 77.21% and 71.38%, respectively, and effectively suppressed biofilm formation by lowering bacterial viscosity and surface hydrophobicity. At a concentration of 0.4 μL/mL, CEO reduced biofilm formation by 40.89% within 24 h, as measured by the crystal violet method. Microscopic and FTIR analysis confirmed CEO's effect on biofilm and extracellular polymer secretion. Thus, it may serve as a novel type of anti-biofilm agent.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-based fermented foods harbor autochthonous lactic acid bacteria with broad antimicrobial activity, bacteriocin diversity, and probiotic potential. 植物性发酵食品含有具有广泛抗菌活性、细菌素多样性和益生菌潜力的原生乳酸菌。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-07-03 DOI: 10.1093/lambio/ovag055

Rine Christopher Reuben, Ojonugwa Precious John, Anayochukwu Chibuike Ngene, Kayode Olayinka Afolabi, Yakubu Aliyu, Silas Dogara Gyar

{"title":"Plant-based fermented foods harbor autochthonous lactic acid bacteria with broad antimicrobial activity, bacteriocin diversity, and probiotic potential.","authors":"Rine Christopher Reuben, Ojonugwa Precious John, Anayochukwu Chibuike Ngene, Kayode Olayinka Afolabi, Yakubu Aliyu, Silas Dogara Gyar","doi":"10.1093/lambio/ovag055","DOIUrl":"10.1093/lambio/ovag055","url":null,"abstract":"Plant-based fermented foods are increasingly recognized as an underexplored niche for autochthonous and beneficial lactic acid bacteria (LAB) with multiple biotechnological and functional potential in health and agrifood systems. We isolated LAB from plant-based fermented foods and assessed their antimicrobial, functional, and probiotic potential using agar-well diffusion technique, in vitro colonization resistance and gastric-environment survivability assays, and 16S rRNA sequencing. Thirteen autochthonous LAB identified as Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, Leuconostoc mesenteroides, Pediococcus acidilactici, Pediococcus pentosaceus, Weissella confusa, Weissella paramesenteroides, and Weissella cibaria demonstrated inhibitory activity against ≥3 pathogens, including Methicillin-resistant Staphylococcus aureus, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, Enterococcus faecium, Enterococcus faecalis, and Pasteurella multocida. The selected LAB strains harbor at least 1 bacteriocin gene, including Plantaricin EF, Plantaricin K, Plantaricin J, Nisin, and Pediocin, and also competitively excluded most pathogens in co-culture experiments, with viable counts ranging from 3.0 to 6.0 log CFU mL-1. Interestingly, the LAB strains showed varying degrees of survivability to simulated gastric conditions, as well as auto- and co-aggregation, hydrophobicity, hydroxyl scavenging, and exopolysaccharide production, and were universally non-hemolytic. Our study demonstrates plant-based fermented foods as notable sources of beneficial LAB with multifunctional biotechnological potential for application in health and agrifood systems.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148277850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High glucose drives metabolic reprogramming toward stress resistance in Zygosaccharomyces mellis. 高糖驱动糖酵母菌的代谢重编程以抵抗逆境。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-07-03 DOI: 10.1093/lambio/ovag057

Tomoko Tamura

{"title":"High glucose drives metabolic reprogramming toward stress resistance in Zygosaccharomyces mellis.","authors":"Tomoko Tamura","doi":"10.1093/lambio/ovag057","DOIUrl":"10.1093/lambio/ovag057","url":null,"abstract":"This study aimed to elucidate transcriptional adaptation mechanisms in Zygosaccharomyces mellis under high-glucose (50%) conditions and to identify cellular processes involved in osmotic stress tolerance. Transcriptomic analysis was performed to characterize global gene expression changes in cells cultured in yeast extract-malt medium containing 50% glucose (G50). Genes involved in glycolysis, including GAL7 and FBP1, were downregulated, whereas PYC2, associated with the tricarboxylic acid cycle (TCA), was upregulated, suggesting suppression of fermentative metabolism and maintenance of respiratory activity. Genes involved in membrane phospholipid synthesis (AGPAT and PLPP) were downregulated, while an acid sphingomyelinase-like gene (ASM) was upregulated, suggesting altered membrane lipid metabolism under osmotic stress. In addition, the cytokinesis-related gene MYO1 was upregulated, suggesting structural reinforcement under osmotic stress. Furthermore, the growth of Δmmr1 and Δhxt4 mutants in Saccharomyces cerevisiae was significantly impaired under G50 conditions, highlighting the importance of mitochondrial inheritance and regulated glucose uptake. These findings suggest that Z. mellis adapts to high-glucose stress by coordinating changes in gene expression associated with respiratory activity, lipid metabolism, and cell division processes.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the macin family of antimicrobial peptides: structure, function, and emerging applications in food safety. 探索抗菌肽的Macin家族：结构、功能和在食品安全中的新应用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-07-03 DOI: 10.1093/lambio/ovag059

Elmesseri Rana, Sorenson Alanna, Bourne G David

{"title":"Exploring the macin family of antimicrobial peptides: structure, function, and emerging applications in food safety.","authors":"Elmesseri Rana, Sorenson Alanna, Bourne G David","doi":"10.1093/lambio/ovag059","DOIUrl":"10.1093/lambio/ovag059","url":null,"abstract":"Rising levels of antimicrobial resistance (AMR) among foodborne pathogens is an increasing threat to food safety and product quality, driving the search for effective natural alternatives to conventional preservatives. Antimicrobial peptides (AMPs) have emerged as promising candidates for food preservation because of their broad-spectrum activity, diverse mechanisms of action and lower propensity for resistance development. Within this group, the macin family represents a promising yet underexplored class of cysteine-rich AMPs characterised by eight conserved cysteine residues that form four disulfide bonds. Macins have been identified across diverse taxa, including leeches, bivalves, gastropods, and hydrozoans and exhibit diverse antimicrobial mechanisms ranging from bacterial aggrelgation mediated by hydrophobic and electrostatic interactions and membrane permeabilization. This review provides a comprehensive synthesis of current knowledge of the macin family within the broader landscape of AMPs, highlighting their structural diversity, mechanisms of action and potential applications. Collectively, these characteristics suggest that macins may represent promising candidates for controlling resistant foodborne pathogens while maintaining product quality.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into antimicrobial resistance and isolation source classification of Cronobacter sakazakii from infant and toddler food in the United States. 美国婴幼儿食品中阪崎克罗诺杆菌抗微生物药物耐药性和分离来源分类的基因组学见解

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-07-03 DOI: 10.1093/lambio/ovag054

Zhao Chen, Meiwen Cui

{"title":"Genomic insights into antimicrobial resistance and isolation source classification of Cronobacter sakazakii from infant and toddler food in the United States.","authors":"Zhao Chen, Meiwen Cui","doi":"10.1093/lambio/ovag054","DOIUrl":"10.1093/lambio/ovag054","url":null,"abstract":"Cronobacter sakazakii is an opportunistic foodborne pathogen associated with severe infections in infants, linked to powdered infant formula (PIF) and related products. We conducted genomic profiling of C. sakazakii (n=209) from infant and toddler food in the United States, comprising all publicly available genomes for this source, through the integration of antimicrobial resistance (AMR) gene (ARG), plasmid replicon, virulence gene, phylogenetic, and pan-genome analyses. We further applied a machine learning (ML)-driven isolation source classification approach based on pan-genome features to distinguish food and clinical isolates. AMR analysis revealed a conserved resistome dominated by three β-lactam resistance genes (blaCSA, blaCSA-1, and blaCSA-2). Independent co-occurrence and pairwise association analysis of ARGs and plasmid replicons indicated sparse and gene-specific relationships, suggesting that observed AMR patterns were more consistent with conserved resistance determinants than extensive plasmid-mediated dissemination. Phylogenetic analysis identified two major clades, while pan-genome assessment demonstrated an open genome dominated by accessory genes. Using gene presence/absence profiles, a random forest classifier achieved high accuracy in distinguishing food and clinical isolates, highlighting the classification power of pan-genome signatures within the dataset. These findings provide insights into the genomic structure of food-associated C. sakazakii and the utility of integrating comparative genomics with ML for food safety surveillance.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-activity relationships of canonical and methyl-substituted isoprenoids delineate determinants of antimetabolic activity in Pseudomonas aeruginosa. 典型类异戊二烯和甲基取代类异戊二烯的构效关系描述了铜绿假单胞菌抗代谢活性的决定因素。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-06-12 DOI: 10.1093/lambio/ovag053

Ramazan Tolga Niçin, Ömer Şimşek

{"title":"Structure-activity relationships of canonical and methyl-substituted isoprenoids delineate determinants of antimetabolic activity in Pseudomonas aeruginosa.","authors":"Ramazan Tolga Niçin, Ömer Şimşek","doi":"10.1093/lambio/ovag053","DOIUrl":"10.1093/lambio/ovag053","url":null,"abstract":"Canonical and non-canonical isoprenoids offer structurally diverse scaffolds for examining how carbon chain length, methyl-substitution pattern, molecular skeleton, and functional group chemistry influence bacterial metabolic activity. In this study, canonical C5/C10 terpenes and novel non-canonical, methyl-substituted C6/C7/C11 isoprenoids were systematically evaluated against the clinical isolate Pseudomonas aeruginosa ESP050. The comparative analysis showed that antimetabolic activity was not governed by carbon number alone. Short-chain non-canonical C6-C7 isoprenoids displayed comparatively stronger and more discriminating suppression profiles than C10-C11 compounds, which exhibited greater compound-specific heterogeneity. Among C7 dimethyl-isoprenol isomers, the position of the methyl substitution significantly influenced activity threshold, recovery dynamics and sustained suppression, indicating that positional isomerism is a key determinant of the antimetabolic phenotype. In the C10 class, the oxygenated linear compound geraniol demonstrated a more prolonged tendency for metabolic suppression compared to the hydrocarbon bicyclic monoterpenes 3-carene and β-pinene. Furthermore, C11 derivatives demonstrated that methylation modulates activity in a scaffold- and position- dependent manner rather than uniformly enhancing efficacy. Overall, these findings indicated that isoprenoid antimetabolic activity arises from the combined effects of chain length, methyl substitution position, molecular skeleton and oxygenation state, supporting the prioritization of selected C6-C7 scaffolds for further antimicrobial evaluation against P. aeruginosa.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptomyces consortia from Chilika Lake suppress pathogenic fungi (Fusarium oxysporum and Rhizoctonia solani) and promote growth in Oryza sativa: potential application as formulation using various carrier materials. 赤利卡湖产的联合链霉菌抑制致病真菌（尖孢镰刀菌和枯丝核菌），促进水稻生长：作为多种载体材料配方的潜在应用

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-06-12 DOI: 10.1093/lambio/ovag052

Subhransu Sekhar Behera, Sourav Ranjan Parida, Lopamudra Ray

{"title":"Streptomyces consortia from Chilika Lake suppress pathogenic fungi (Fusarium oxysporum and Rhizoctonia solani) and promote growth in Oryza sativa: potential application as formulation using various carrier materials.","authors":"Subhransu Sekhar Behera, Sourav Ranjan Parida, Lopamudra Ray","doi":"10.1093/lambio/ovag052","DOIUrl":"10.1093/lambio/ovag052","url":null,"abstract":"The genus Streptomyces encompasses diverse strains capable of producing antifungal compounds that combat various soil pathogens, responsible for reduced rice yields. The primary aim of this research was to investigate the potential of a consortium comprising four Streptomyces species, specifically Streptomyces chilikensis RC1830, Streptomyces barkulensis RC1831, Streptomyces chitinovorans RC1832, and Streptomyces griseoincarnatus RB7AG, isolated from the Chilika Lake estuary, against Fusarium oxysporum and Rhizoctonia solani. The consortia exhibited significant antagonistic activity against both soil-borne pathogens. In addition, inoculation with the consortium increased the shoot length by 40%, root length by 47.2%, total chlorophyll by 47.5%, and shoot dry weight 45.3% compared to the control. The disease severity index (DSI) was also reduced by approximately 50% following application of the Streptomyces consortium. Formulation trials revealed that formulations prepared using vermiculite, activated charcoal, and biochar were most effective in maintaining viable propagule counts, greatest shelf life when stored at 4°C (∼40-60 × 106 CFU mL⁻¹ after 3 months). These findings indicate that Streptomyces-based consortia can be efficiently utilized for sustainable disease management and growth promotion in rice.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An applied framework of CLSM and rheology for controlling foodborne biofilms and their cleaning agents and disinfectants resistance: a review. CLSM和流变学在控制食源性生物膜及其对清洗剂和消毒剂的耐药性中的应用框架

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2026-06-12 DOI: 10.1093/lambio/ovag047

Haoyue Xue, Yanchao Chen

{"title":"An applied framework of CLSM and rheology for controlling foodborne biofilms and their cleaning agents and disinfectants resistance: a review.","authors":"Haoyue Xue, Yanchao Chen","doi":"10.1093/lambio/ovag047","DOIUrl":"10.1093/lambio/ovag047","url":null,"abstract":"Biofilms formed by foodborne pathogens pose a significant threat to food safety, as they enhance microbial resistance to disinfectants and environmental stress. Understanding the relationship between their intricate structure and macroscopic mechanical properties is crucial for developing effective control strategies. This review systematically investigates how the combined application of confocal laser scanning microscopy (CLSM) and rheology provides a powerful solution to this problem. CLSM technology reveals biofilm structure and extracellular polymeric substance (EPS) distribution through high-resolution 3D imaging and multi-component fluorescence labeling. Rheology quantitatively analyzes key mechanical parameters such as viscoelasticity and yield stress. Their integration establishes a multidimensional \"structure-function-mechanics\" research framework, enabling cross-scale correlations from microscopic features to macroscopic mechanical responses. Additionally, this review analyzes current biofilm removal strategies, fluorescent labeling methods for EPS components, and the composition and functions of EPS in typical bacterial biofilms. We further explore optimized technical approaches to overcome food matrix interference, applications in cleaning process optimization, and future standardization directions integrated with artificial intelligence. This comprehensive methodology provides a systematic and efficient paradigm for understanding biofilm mechanisms and developing control strategies, holding significant implications for biofilm prevention in the food industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0