Native bradyrhizobia for soybean: genetic and functional diversity in Heihe soils, a major production zone of Heilongjiang, China.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI:10.1093/lambio/ovag026

Ke Li, Keyu Chen, Haoran Hao, Kaiwen Zhang, Brigitte Brunel, Wenshan Zhou, Junjie Zhang

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引用次数: 0

Abstract

Soybean (Glycine max) forms symbiotic nitrogen fixation with rhizobia, and compatible, efficient rhizobia in soils are vital for its sustainable production. This study analyzed the distribution and traits of native soybean-associated rhizobia in soils from Heihe, Heilongjiang Province, to identify strains with high symbiotic nitrogen fixation efficiency and environmental adaptability, and select candidates for local microbial inoculants to boost sustainable soybean production. Seventy-four rhizobial isolates were obtained from three Heilongjiang sampling sites using a local soybean variety, and characterized genetically and symbiotically. PCR-RFLP of IGS DNA grouped them into 9 genotypes. Multilocus sequence analysis (16S rRNA, recA, atpD, gyrB genes) placed representative strains into three known species (Bradyrhizobium japonicum, B. diazoefficiens, B. ottawaense) and an uncharacterized Bradyrhizobium group (64%, dominant). Phylogeny of nodC/nifH markers showed affiliation with symbiovar glycinearum. All strains nodulated soybean with symbiotic efficiency (67-88%); about half enhanced plant biomass. Three strains (DG28, GCZ12, SH16) showing superior symbiotic efficiency. Representative strains had varied tolerance to alkalinity, high temperature, and PEG-induced drought. Strain SH16 combined high efficiency and stress tolerance. These results provide promising candidates for local soybean inoculant development.

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大豆原生缓生根瘤菌：黑龙江省主要产区黑河土壤的遗传和功能多样性

大豆（Glycine max）与根瘤菌形成共生固氮，土壤中相容、高效的根瘤菌对大豆的可持续生产至关重要。本研究分析了黑龙江省黑河地区土壤中大豆伴生根瘤菌的分布和性状，以鉴定具有高共生固氮效率和环境适应性的菌株，为大豆可持续生产选择适宜的本地微生物接种剂。利用黑龙江省一个大豆品种从3个取样点分离出74株根瘤菌，并进行了遗传和共生鉴定。IGS DNA的PCR-RFLP将其分为9个基因型。多位点序列分析（16S rRNA, recA, atpD， gyrB基因）将代表性菌株分为三个已知种（日本慢生根瘤菌，重氮根瘤菌，ottawawense）和一个未鉴定的慢生根瘤菌群（64%，优势）。nodC/nifH标记在系统发育上与glycinearum有亲缘关系。所有菌株结瘤大豆的共生效率为67 ~ 88%；增加了约一半的植物生物量。3株菌株DG28、GCZ12、SH16表现出较好的共生效率。代表性菌株对碱性、高温和peg诱导的干旱具有不同的耐受性。应变SH16结合了高效率和耐应力性。这些结果为当地大豆接种剂的开发提供了有希望的候选材料。

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.