Allelic exchange technologies for Clostridioides difficile research and their application in toxin studies.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Terry W Bilverstone
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引用次数: 0

Abstract

The ability to precisely manipulate the genome of Clostridioides difficile allows for the generation of mutants that can be characterized to unveil critical aspects relating to pathogenesis and virulence. Such findings can ultimately direct the development of antivirulence agents to combat C. difficile infection, by small molecule inhibition. In this review, the mechanisms underpinning the most widely adopted allelic exchange technologies for generating precise genomic mutations in C. difficile, are discussed. The advantages and disadvantages of each system are considered. In addition, a comprehensive review of the application of these tools for the study of C. difficile toxins and genes residing within the toxin loci, is presented.

用于艰难梭菌研究的等位基因交换技术及其在毒素研究中的应用。
精确操纵艰难梭菌基因组的能力允许突变体的产生,这些突变体可以被描述为揭示与发病机制和毒力有关的关键方面。这些发现最终可以指导抗毒剂的发展,通过小分子抑制来对抗艰难梭菌感染。在这篇综述中,讨论了在艰难梭菌中产生精确基因组突变的最广泛采用的等位基因交换技术的机制。考虑了每种系统的优缺点。此外,对这些工具在艰难梭菌毒素和毒素位点内的基因研究中的应用进行了全面的综述。
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来源期刊
Letters in Applied Microbiology
Letters in Applied Microbiology 工程技术-生物工程与应用微生物
CiteScore
4.40
自引率
4.20%
发文量
225
审稿时长
3.3 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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