用于艰难梭菌研究的等位基因交换技术及其在毒素研究中的应用。

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI:10.1093/lambio/ovaf034

Terry W Bilverstone

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Allelic exchange technologies for Clostridioides difficile research and their application in toxin studies.

The ability to precisely manipulate the genome of Clostridioides difficile allows for the generation of mutants that can be characterized to unveil critical aspects relating to pathogenesis and virulence. Such findings can ultimately direct the development of antivirulence agents to combat C. difficile infection, by small molecule inhibition. In this review, the mechanisms underpinning the most widely adopted allelic exchange technologies for generating precise genomic mutations in C. difficile, are discussed. The advantages and disadvantages of each system are considered. In addition, a comprehensive review of the application of these tools for the study of C. difficile toxins and genes residing within the toxin loci, is presented.

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来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.