Stress resistance of the biofilm and planktonic forms of Lactobacillus delbrueckii in adverse environments.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jiangyang Zhai, Lili Nie, Jianjun Tian, Yinfeng He, Yue Gu
{"title":"Stress resistance of the biofilm and planktonic forms of Lactobacillus delbrueckii in adverse environments.","authors":"Jiangyang Zhai, Lili Nie, Jianjun Tian, Yinfeng He, Yue Gu","doi":"10.1093/lambio/ovaf031","DOIUrl":null,"url":null,"abstract":"<p><p>Biofilm and planktonic forms are different kinds of self-protection mechanisms in microorganisms for resistance to adverse environments. The research explored the physicochemical properties, antimicrobial, and antioxidant activities of Lactobacillus delbrueckii (L. delbrueckii) 5E, focusing on the biofilm's stress tolerance. L. delbrueckii 5E's high lactic acid production (709.1 g·L-1) enhances its antimicrobial activity, which is pH-dependent and decreases when the supernatant is adjusted to pH 6.5. The cell-free fermentation supernatant, bacterial suspension, and cell-free extract of L. delbrueckii 5E showed significant scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion radicals. L. delbrueckii 5E formed biofilms with a bacterial count of 9.00 × 106 CFU·mL-1 on polyacrylonitrile electrospun membranes. Stress-tolerance tests indicated that the biofilm form of L. delbrueckii 5E exhibited superior survival under high temperatures, osmotic pressures, bile salts, potassium sorbate, and H2O2 exposure. The biofilm consistently released free bacteria, maintaining a stable total colony count of 106 CFU·mL-1. The remarkable antibacterial and antioxidant properties of L. delbrueckii 5E, along with its resilience to harsh environments, establish its potential for applications in the food industry.</p>","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Letters in Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/lambio/ovaf031","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Biofilm and planktonic forms are different kinds of self-protection mechanisms in microorganisms for resistance to adverse environments. The research explored the physicochemical properties, antimicrobial, and antioxidant activities of Lactobacillus delbrueckii (L. delbrueckii) 5E, focusing on the biofilm's stress tolerance. L. delbrueckii 5E's high lactic acid production (709.1 g·L-1) enhances its antimicrobial activity, which is pH-dependent and decreases when the supernatant is adjusted to pH 6.5. The cell-free fermentation supernatant, bacterial suspension, and cell-free extract of L. delbrueckii 5E showed significant scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion radicals. L. delbrueckii 5E formed biofilms with a bacterial count of 9.00 × 106 CFU·mL-1 on polyacrylonitrile electrospun membranes. Stress-tolerance tests indicated that the biofilm form of L. delbrueckii 5E exhibited superior survival under high temperatures, osmotic pressures, bile salts, potassium sorbate, and H2O2 exposure. The biofilm consistently released free bacteria, maintaining a stable total colony count of 106 CFU·mL-1. The remarkable antibacterial and antioxidant properties of L. delbrueckii 5E, along with its resilience to harsh environments, establish its potential for applications in the food industry.

德氏乳杆菌生物膜和浮游形式在不利环境下的抗逆性。
生物膜形式和浮游形式是微生物抵抗不利环境的不同的自我保护机制。本研究探讨了德布鲁克氏乳杆菌5E的理化性质、抗菌和抗氧化活性,重点研究了其生物膜的耐受性。L. delbrueckii 5E的高乳酸产量(709.1 g·L-1)增强了其抑菌活性,其抑菌活性与pH有关,当上清液调整到pH 6.5时,抑菌活性降低。delbrueckii 5E的无细胞发酵上清液、细菌悬浮液和无细胞提取物对DPPH、羟基和超氧阴离子自由基具有明显的清除活性。L. delbrueckii 5E在聚丙烯腈静电纺丝膜上形成细菌数为9.00 × 106 CFU·mL-1的生物膜。胁迫耐受性试验表明,delbrueckii 5E的生物膜形式在高温、渗透压、胆盐、山梨酸钾和H2O2暴露下表现出优越的存活率。生物膜持续释放游离菌,菌落总数稳定在106 CFU·mL-1。L. delbrueckii 5E具有显著的抗菌和抗氧化性能,以及对恶劣环境的适应能力,在食品工业中具有应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Letters in Applied Microbiology
Letters in Applied Microbiology 工程技术-生物工程与应用微生物
CiteScore
4.40
自引率
4.20%
发文量
225
审稿时长
3.3 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信