Letters in Applied Microbiology最新文献_第2页

The contribution of irrigation water and growth substrate for microbial flux in a vertical farm. 灌溉水和生长基质对垂直农场微生物通量的贡献。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf093

Elliot Erskine, Niall Skinner, Nicola Holden

{"title":"The contribution of irrigation water and growth substrate for microbial flux in a vertical farm.","authors":"Elliot Erskine, Niall Skinner, Nicola Holden","doi":"10.1093/lambio/ovaf093","DOIUrl":"10.1093/lambio/ovaf093","url":null,"abstract":"Controlled environmental agriculture (CEA) is an emerging technology with increasing adoption for commercial applications. However, its impact on the plant microbiome is not entirely clear. The assumption is that controlled conditions reduce the risk of introduction and spread of pathogens, human or plant. Here, we assessed the microbial flux through a commercially relevant CEA plant growth tower from culture-dependent and independent approaches. This allowed the relationship between two of the main entry points for microbes to be determined, the circulating water system and plant growth substrates, on two crop species systems, kale and lettuce. There was a clear distinction between the taxonomic compositions of bacteria in the water-associated and coir-associated compartments. Overall, water did not contribute the most abundant members of the microbiota on plants. Rainwater, used as a top-up source of water, was not the major source of sequenced microbes in either the circulating water system or in coir compartments. The main points of expansion of cultural microbes were in the irrigation tray system and the physical presence and growth of the crop plants. The effect of UV-C, typically used to treat water, and the LED lighting system were quantified for proxy pathogen strains.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cervical cancer pathology and vaginal and gut microbiota: conception of the association. 子宫颈癌病理与阴道和肠道微生物群：关联的概念。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf088

Samaneh Mazinani, Mohammad Aghazadeh, Vahdat Poortahmasebi, Vahid Arafi, Alka Hasani

{"title":"Cervical cancer pathology and vaginal and gut microbiota: conception of the association.","authors":"Samaneh Mazinani, Mohammad Aghazadeh, Vahdat Poortahmasebi, Vahid Arafi, Alka Hasani","doi":"10.1093/lambio/ovaf088","DOIUrl":"10.1093/lambio/ovaf088","url":null,"abstract":"The role of gut and vaginal microbiota has been examined as potential markers in the progression of cervical cancer and inspired heightened attention. We attempted to identify the current status, the influence of changing trends in the gut and vaginal microbiota, and their impact on cervical cancer. All publications were searched in online databases such as PubMed, Scopus, Medline, and Google Scholar using the keywords \"cervical cancer,\" \"vaginal microbiota,\" \"gut microbiota,\" \"dysbiosis,\" \"cervical intraepithelial neoplasia,\" and \"HPV.\" Growing evidence that gut microbiota dysbiosis is involved in many diseases has attracted many researchers. The vaginal microbiome is a micro-ecosystem complex that undergoes continuous fluctuations throughout a woman's life. Decrease in Lactobacilli spp. Levels can lead to an overgrowth of anaerobic bacteria, resulting in bacterial vaginosis that accounts for adverse health outcomes, including increased susceptibility to sexually transmitted infections, including human papillomavirus, and cervical intraepithelial neoplasia, leading to cervical cancer. Thus, dysbiosis, characterized by an imbalance of beneficial and harmful bacteria, can negatively affect health. Understanding the intricate interactions between the microbiota and cervical cancer can provide valuable insights into potential therapeutic strategies and the development of preventive measures.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paulownin triazole-chloroquinoline derivative: a promising antiviral candidate against chikungunya virus. 泡桐碱三唑-氯喹啉衍生物：一种有前途的基孔肯雅病毒抗病毒药物。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf092

Adriana Cotta Cardoso Reis, Camila Mendes de Oliveira, Beatriz Carvalho Rangel, Laura Vogas Bonsucesso de Carvalho, Camila Portruneli, Livia da Cunha Agostini, Isadora Oliveira Ansaloni Pereira, Breno de Mello Silva, Cíntia Lopes de Brito Magalhães, Glenda Nicioli da Silva, Guilherme Rocha Pereira, Geraldo Célio Brandão

{"title":"Paulownin triazole-chloroquinoline derivative: a promising antiviral candidate against chikungunya virus.","authors":"Adriana Cotta Cardoso Reis, Camila Mendes de Oliveira, Beatriz Carvalho Rangel, Laura Vogas Bonsucesso de Carvalho, Camila Portruneli, Livia da Cunha Agostini, Isadora Oliveira Ansaloni Pereira, Breno de Mello Silva, Cíntia Lopes de Brito Magalhães, Glenda Nicioli da Silva, Guilherme Rocha Pereira, Geraldo Célio Brandão","doi":"10.1093/lambio/ovaf092","DOIUrl":"10.1093/lambio/ovaf092","url":null,"abstract":"Viral infections, including arboviruses such as chikungunya, zika, dengue, and mayaro fever, remain significant global health and economic challenges, fueled by the emergence and resurgence of mosquito-borne diseases. Natural products, especially plant-derived compounds, have been crucial in drug discovery and often serve as scaffolds for synthetic drug development. This study focused on modifying paulownin, an isolated lignan, through a Click reaction to incorporate 1,2,3-triazole and quinolinic ring frameworks. The resulting derivative 7 was evaluated for its in vitro antiviral activity against Alphavirus chikungunya (CHIKV). The paulownin derivative 7 did not exhibit cytotoxicity in Vero cells and demonstrated potent activity against CHIKV, with median effective concentration value of 9.05 µM and a selectivity index exceeding 16.8. Furthermore, compound 7 outperformed positive controls, being over 46 times more active against CHIKV. Cytopathic effect assays confirmed this anti-CHIKV activity. The virucidal assay indicated that the compound does not exert a direct effect on CHIKV particles before cell infection. RT-qPCR studies further demonstrated derivative 7 significantly reduces CHIKV replication. These findings highlight the paulownin derivative 7 as a promising and selective candidate for CHIKV treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bactericidal and anti-biofilm activity of ebastine against Staphylococcus aureus. 依巴斯汀对金黄色葡萄球菌的杀菌和抗生物膜活性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-07-02 DOI: 10.1093/lambio/ovaf086

Liping Zhao, Hui Zhang, Li Zha, Xicheng Zhou, Meng Yang

{"title":"Bactericidal and anti-biofilm activity of ebastine against Staphylococcus aureus.","authors":"Liping Zhao, Hui Zhang, Li Zha, Xicheng Zhou, Meng Yang","doi":"10.1093/lambio/ovaf086","DOIUrl":"10.1093/lambio/ovaf086","url":null,"abstract":"Drug repurposing, offers promising opportunities to address infections caused by multidrug-resistant bacteria. This study was to evaluate the bactericidal activity, anti-biofilm properties, and potential mechanisms of the antihistamine drug ebastine against Staphylococcus aureus. The minimum inhibitory concentrations of ebastine against standard and clinical S. aureus isolates were determined using the broth microdilution method. The MIC values ranged from 2 to 8 µg·mL-1, indicating good activity against clinical drug-resistant strains. Time-kill curve analyses revealed a dose-dependent bactericidal effect. Regarding anti-biofilm activity, ebastine significantly inhibited biofilm formation at higher concentrations and demonstrated a moderate ability to eradicate preformed biofilms. Mechanistic studies revealed that ebastine exerted the antimicrobial effects by causing disruption to bacterial membrane integrity and inducing reactive oxygen species generation. Furthermore, safety evaluations showed that ebastine exhibited limited toxicity to mammalian cells, with negligible hemolytic effects and good overall safety profiles. This study provided new insights into the potential applications of ebastine in the field of antimicrobial therapy, highlighting its promise as a non-traditional antibacterial agent.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of a bacterial lysate on composition of the lung microbiota in a mouse model of asthma exposed to titanium dioxide as one of air pollutants. 细菌裂解物对暴露于二氧化钛作为空气污染物之一的哮喘小鼠模型中肺微生物群组成的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf089

Da-Yeon Hwang, Min-Hyeok An, Pureun-Haneul Lee, Ae-Rin Baek, Shinhee Park, Yunha Nam, June-Hyuk Lee, Sung-Woo Park, Do-Jin Kim, An-Soo Jang

{"title":"The impact of a bacterial lysate on composition of the lung microbiota in a mouse model of asthma exposed to titanium dioxide as one of air pollutants.","authors":"Da-Yeon Hwang, Min-Hyeok An, Pureun-Haneul Lee, Ae-Rin Baek, Shinhee Park, Yunha Nam, June-Hyuk Lee, Sung-Woo Park, Do-Jin Kim, An-Soo Jang","doi":"10.1093/lambio/ovaf089","DOIUrl":"https://doi.org/10.1093/lambio/ovaf089","url":null,"abstract":"Environmental microbes have been linked to both beneficial and harmful effects on the lungs in relation to asthma. The aim of our study was to characterize the bacterial microbiome in lung samples from a mouse model of asthma exposed to titanium dioxide (TiO2) particles, and the effect of OM-85 Broncho-Vaxom®, a bacterial lysate on lung microbiome in mice. The mice were divided randomly into four groups of 6-8 mice per group. To identify the microbial communities in lung samples the upper right lung of all groups, we amplified and sequenced the rRNA gene regions from bacterial DNA (16S). The amplified 16S region was sequenced using the Roche-454 Life Sciences Titanium pyrosequencing platform. In the OVA + TiO2 + OM-85 group, airway hyperresponsiveness and inflammatory cells in bronchoalveolar lavage fluid (BALF) decreased compared to the OVA + TiO2 group. Inflammatory cytokine levels were lower in the OVA + TiO2 + OM-85 group. Certain bacteria, and Collinsella aerofaciens, decreased in the OVA + TiO2 + OM-85 group, while Neisseria perflava and Fusobacterium periodonticum increased. Lactic acid bacteria groups decreased in the OVA + TiO2 + OM-85 group. TiO2 particles exposure changed lung microbial taxa, and modified by a bacterial lysate, suggesting that a probiotic can be helpful in reducing exacerbation of airway disease exposed to air pollutants.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are foliar extracts of mycorrhizal Hymenaea martiana toxic to human peripheral blood mononuclear cells? 菌根膜菌叶提取物对人外周血单核细胞有毒性吗？

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf075

Brena Coutinho Muniz, Carlos André Ribeiro Costa, Caio Bezerra Barreto, Jefferlone Lopes da Silva Filho, Maria Rafaele Oliveira Bezerra da Silva, Silvana de Fátima Ferreira da Silva, Daniela de Araújo Viana Marques, Qiang-Sheng Wu, Fábio Sérgio Barbosa da Silva

{"title":"Are foliar extracts of mycorrhizal Hymenaea martiana toxic to human peripheral blood mononuclear cells?","authors":"Brena Coutinho Muniz, Carlos André Ribeiro Costa, Caio Bezerra Barreto, Jefferlone Lopes da Silva Filho, Maria Rafaele Oliveira Bezerra da Silva, Silvana de Fátima Ferreira da Silva, Daniela de Araújo Viana Marques, Qiang-Sheng Wu, Fábio Sérgio Barbosa da Silva","doi":"10.1093/lambio/ovaf075","DOIUrl":"10.1093/lambio/ovaf075","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are known to enhance the accumulation of bioactive compounds with medicinal properties in plants. However, the potential cytotoxic effects of extracts from mycorrhizal plants on peripheral blood mononuclear cells (PBMC) remain unexplored. Therefore, this study aimed to verify the cytotoxic potential of foliar extract from Hymenaea martiana Hayne seedlings, either associated or not associated with AMF on PBMC. A greenhouse experiment was conducted with two treatments: a control group (without AMF) and a group inoculated with Acaulospora longula Spain and N.C. Schenck. After 148 days, leaves were collected to prepare aqueous extracts, and cytotoxicity of the extracts was assessed using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay. Additionally, the antioxidant potential and the content of soluble carbohydrates, flavonoids, flavonols, flavonones, dihydroflavonols, and saponins were assessed. Hymenaea martiana seedlings associated with A. longula exhibited a more than 50% increase in the accumulation of phenolic compounds compared to the control. However, no toxicity was detected for PBMC under any of the conditions evaluated. This study provides the first evidence of the effect of mycorrhizal plant extracts on human blood cells, highlighting their potential safety for medicinal and cosmetic applications.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual role of Bacillus thuringiensis RZ2MS9: its effectiveness as an entomopathogen and a plant growth promoter in vitro and in field conditions. 苏云金芽孢杆菌RZ2MS9在体外和田间条件下的双重作用：昆虫病原和植物生长促进剂。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf076

Daniel Prezotto Longatto, Adriana Sturion Lorenzi, Helena Gutierrez Oliveira, Mauricio Santos Rosa, Joelma Marcon, João Lucio Azevedo, Maria Carolina Quecine

{"title":"The dual role of Bacillus thuringiensis RZ2MS9: its effectiveness as an entomopathogen and a plant growth promoter in vitro and in field conditions.","authors":"Daniel Prezotto Longatto, Adriana Sturion Lorenzi, Helena Gutierrez Oliveira, Mauricio Santos Rosa, Joelma Marcon, João Lucio Azevedo, Maria Carolina Quecine","doi":"10.1093/lambio/ovaf076","DOIUrl":"10.1093/lambio/ovaf076","url":null,"abstract":"Bacillus thuringiensis RZ2MS9, a plant growth-promoting rhizobacterium isolated from the rhizosphere of guarana plants, has shown significant growth enhancement in both soybean and maize crops. To explore its full potential, we investigated RZ2MS9's entomopathogenic properties against Lepidoptera and Coleoptera larvae in vitro, and against Hemiptera populations in both in vitro and field conditions. The strain was evaluated for insecticidal crystal proteins, which were found in cuboid and spherical forms. Comparative genomic analysis revealed that while RZ2MS9 did not share significant pesticidal protein genes with B. thuringiensis HD1, it has unique genes related to plant growth promotion and nutrient acquisition. RZ2MS9 also exhibited chitinolytic activity, linked to the presence of the chitosanase coding gene (chP). Under laboratory conditions, the larvae mortality under RZ2MS9 treatment was 90% for sugarcane borer (Diatraea saccharalis), 92.5% for old-world cotton bollworm (Helicoverpa armigera), 30% for Agrotis ipsilon, and 87.5% for Anthonomus grandis. In addition, RZ2MS9 caused severe wing deformities in 40% of Spodoptera frugiperda moths and repelled Piezodorus guildinii from its food source. In field trials, soybean plants inoculated with RZ2MS9 exhibited significative increased length. P. guildinii was more prevalent in the inoculated plants, while the incidence of Euchistus heros remained unchanged.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Singlet oxygen antioxidant capacity of carotenoids from Halorubrum salinarum. 盐碱地类胡萝卜素单线态氧抗氧化能力研究。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf085

Nestly Anne M Cruz-Angeles, Mark Kevin P Devanadera, Kenshi Watanabe, Reuel M Bennett, Tsunehiro Aki, Gina R Dedeles

引用次数: 0

In vitro and in-planta bioefficacy of bacteriome isolated from diseased leaf lesions of two contrasting rice genotypes against sheath blight disease of rice. 两种不同基因型水稻叶片病变菌群对水稻纹枯病的体外和体内生物药效研究

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf078

Indrajeet Usha, Devanna Pramesh, Adke Raghunandana, Muthakapalli Krishnareddy Prasannakumar, Gururaj Sunkad, Channappa Manjunatha

{"title":"In vitro and in-planta bioefficacy of bacteriome isolated from diseased leaf lesions of two contrasting rice genotypes against sheath blight disease of rice.","authors":"Indrajeet Usha, Devanna Pramesh, Adke Raghunandana, Muthakapalli Krishnareddy Prasannakumar, Gururaj Sunkad, Channappa Manjunatha","doi":"10.1093/lambio/ovaf078","DOIUrl":"10.1093/lambio/ovaf078","url":null,"abstract":"Rice leaves harbor a diverse agriculturally important bacteriome. Using different dilutions and media, 20 bacterial isolates were obtained from the diseased and healthy leaves of two rice cultivars (cv. BPT-5204 and cv. Tetep). Later, the taxonomy was deduced based on the 16S rDNA sequences, which revealed 17 bacterial species belonging to 13 genera. The antifungal activity of 17 bacteria was tested against Rhizoctonia solani, which showed that Bacillus cereus, Stenotrophomonas maltophilia, and Acinetobacter lwoffii exhibited the mycelial inhibition of 65.18%, 61.85%, and 61.85%, respectively. Further, six selected bacteria (treatments: T1-T6) in three different methods of inoculation (methods: M1-M3) were tested on-field against sheath blight (ShB) for two consecutive Kharif seasons (2022 and 2023). The two-season data indicated a statistically insignificant effect of methods on the % disease index (PDI) (40.51-42.47), whereas different bacteria treatments (T1-T6) showed a significant impact on PDI (29.15-52.08). S. maltophilia isolated from the diseased BPT-5204 was superior to all other strains in reducing the PDI (29.15). We successfully isolated and functionally characterized 17 bacteria from the artificially diseased contrasting genotypes. This study demonstrated that rice lesions naturally harbor a diverse bacterium with antifungal effects that can be used to develop non-chemical-based disease management strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multilocus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme. 新兴呼吸道病原体双形支原体的多位点序列分型方案。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf073

Lily Simpson, Joshua Macleod, Richard S Rowlands, Owen B Spiller, Charlotte Dixon, Jorgen S Jensen, Baharak Afshar, Helena M B Seth-Smith, Victoria J Chalker, Michael L Beeton

{"title":"A multilocus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme.","authors":"Lily Simpson, Joshua Macleod, Richard S Rowlands, Owen B Spiller, Charlotte Dixon, Jorgen S Jensen, Baharak Afshar, Helena M B Seth-Smith, Victoria J Chalker, Michael L Beeton","doi":"10.1093/lambio/ovaf073","DOIUrl":"10.1093/lambio/ovaf073","url":null,"abstract":"Mycoplasma amphoriforme is an emerging respiratory pathogen for which little is known about the population structure or transmission dynamics. In this study, we developed the first multilocus sequence typing (MLST) scheme for M. amphoriforme and applied it to a previous genomic data set. The genomes of seven M. amphoriforme isolates from the UK and Denmark were sequenced and used to develop the MLST scheme based on loci used for previous Mycoplasma MLST schemes. The resulting MLST scheme consisted of four loci (gyrB, atpG, uvrA, and rpoB) and was applied to 20 previously sequenced genomes obtained from the UK and France/Tunisia. From the 27 sequences examined, 13 sequence types were identified. A phylogenetic tree of concatenated sequences showed a comparable topology to a previously described tree based on whole genome data. Additionally, the MLST scheme corroborated the previous suggestion of possible healthcare-associated transmission of M. amphoriforme between two separate patients. The MLST scheme gave a population structure analysis comparable to previous whole-genome-based analyses.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0