Letters in Applied Microbiology最新文献_第2页

Hydrocleys nymphoides (water poppy): a cosmopolitan aquatic plant with a phytochemical-rich extract exhibiting antimicrobial and antibiofilm activity against multidrug-resistant Staphylococcus. 水罂粟：一种世界性的水生植物，具有丰富的植物化学物质提取物，对多重耐药葡萄球菌具有抗菌和抗生物膜活性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-04-01 DOI: 10.1093/lambio/ovaf046

Marcos Toneto Francisco, Pedro Henrique de Jesus Barbosa, Faizan Ahmad, Sérgio Antônio Fernandes, Marcia Giambiagi-deMarval, Marisa Alves Nogueira Diaz, Ciro César Rossi

{"title":"Hydrocleys nymphoides (water poppy): a cosmopolitan aquatic plant with a phytochemical-rich extract exhibiting antimicrobial and antibiofilm activity against multidrug-resistant Staphylococcus.","authors":"Marcos Toneto Francisco, Pedro Henrique de Jesus Barbosa, Faizan Ahmad, Sérgio Antônio Fernandes, Marcia Giambiagi-deMarval, Marisa Alves Nogueira Diaz, Ciro César Rossi","doi":"10.1093/lambio/ovaf046","DOIUrl":"10.1093/lambio/ovaf046","url":null,"abstract":"Antimicrobial resistance among Staphylococcus species, including multidrug-resistant and biofilm-forming strains, poses a critical threat to global health, demanding innovative therapeutic solutions. In this context, this study explores the antimicrobial and antibiofilm potential of the aquatic plant Hydrocleys nymphoides as a promising alternative. Extracts from the plant's leaves and roots were obtained using solvents of increasing polarity and tested against five key pathogenic Staphylococcus species: S. aureus, S. epidermidis, S. haemolyticus, S. pseudintermedius, and S. coagulans. The hexane extract of H. nymphoides leaves showed the most notable activity, with inhibition zones of 9-17 mm and minimum inhibitory concentrations (MICs) as low as 0.8 mg/ml for certain strains. Subinhibitory concentrations of the extract significantly reduced biofilm formation in most strains, with reductions up to 46.9%. Gas chromatography-mass spectrometry revealed bioactive compounds such as linoleic acid, n-hexadecanoic acid, 9-octadecenal, eicosane, and tetratriacontane, known for their antimicrobial and antibiofilm properties. Although cytotoxicity was observed at concentrations near the MIC, lower concentrations were non-toxic, indicating potential for controlled therapeutic applications. These findings underscore the biotechnological value of H. nymphoides as a sustainable source of antimicrobial agents against multidrug-resistant Staphylococcus. This work emphasizes the critical role of phytotherapy in combating the escalating antimicrobial resistance crisis.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of fermentation conditions for enhancing the antioxidant activity of medicine and food homologous (MFHs) materials using Lactobacillus plantarum. 植物乳杆菌提高药物和食品同源材料抗氧化活性的发酵条件优化。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-29 DOI: 10.1093/lambio/ovaf051

Yikai Wang, Qiming Chen, Zhengrong Lu, Quanling Dong, Jiaxu Wang, Yuanlong Hu, Tiantian Tang, Zhanmin Liu

{"title":"Optimization of fermentation conditions for enhancing the antioxidant activity of medicine and food homologous (MFHs) materials using Lactobacillus plantarum.","authors":"Yikai Wang, Qiming Chen, Zhengrong Lu, Quanling Dong, Jiaxu Wang, Yuanlong Hu, Tiantian Tang, Zhanmin Liu","doi":"10.1093/lambio/ovaf051","DOIUrl":"https://doi.org/10.1093/lambio/ovaf051","url":null,"abstract":"Codonopsis radix, Astragalus membranaceus, Platycodon grandiflorus, and Tiger milk mushroom are promising candidates for functional food development. However, their limited bioavailability impedes a challenge to broader applications. In this study, fermentation of these medicine and food homologous (MFHs) materials using Lactobacillus plantarum was optimized to enhance antioxidant activity. Through single-factor experiments and Pareto analysis, the key factors influencing DPPH radical scavenging activity, including the material-to-water ratio, sucrose concentration, and inoculum size, were identified. Response surface methodology and Artificial neural networks were then employed to optimize fermentation parameters. The optimal conditions resulted in a DPPH radical scavenging rate of 95.8%. Furthermore, the levels of bioactive compounds, including polysaccharides, polyphenols, flavonoids, and saponins, were significantly elevated, which enhanced antioxidant activity and may have improved bioavailability. FTIR analysis confirmed the structural changes in the active compounds after fermentation. This study provides valuable insights into developing MFH-based probiotic fermented products, enhancing their nutritional and biological properties.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing Microbial Functionalities and Ecological Roles in Rajpardi Lignite Mine: Insights from Metagenomics Analysis. 揭示Rajpardi褐煤矿微生物功能和生态作用：来自宏基因组学分析的见解。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-28 DOI: 10.1093/lambio/ovaf048

Anjana Sanghani, Komal Antaliya, Rajesh Patel, Shailesh Dave, Devayani Tipre

{"title":"Revealing Microbial Functionalities and Ecological Roles in Rajpardi Lignite Mine: Insights from Metagenomics Analysis.","authors":"Anjana Sanghani, Komal Antaliya, Rajesh Patel, Shailesh Dave, Devayani Tipre","doi":"10.1093/lambio/ovaf048","DOIUrl":"https://doi.org/10.1093/lambio/ovaf048","url":null,"abstract":"The present study employs a metagenomics approach to evaluate microbial communities' ecological functions and potential within the Rajpardi lignite mine of Gujarat, India. Through whole genome shotgun sequencing on the Illumina Miseq platform, we obtained 10,071,318 sequences, which unveiled a diverse and abundant microbial community primarily composed of Proteobacteria, Acidobacteria, and Nitrospirae. Comprehensive taxonomic profiling and gene prediction was carried out using the SqueezeMeta pipline, which highlighted significant contributions to carbohydrate, amino acid, and energy metabolism. The detection of antimicrobial resistance (AMR) and stress resistance genes, such as blaTEM and merA, suggests that these microbes possess the ability to adapt to harsh environmental conditions. Genome binning revealed species such as Acidiphilum sp. 20-67-58, emphasising the nature of these communities as they adapted to an acidic environment. This finding highlights the crucial role of microbes in biogeochemical cycles, emphasizing their potential in bioremediation, pollutant degradation, and ecosystem restoration.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Fe and Mn in organo-mineral-microbe interactions: evidence of carbon stabilization and transformation of organic matter leading to carbon greenhouse gas emissions. 铁和锰在有机矿物-微生物相互作用中的作用：碳稳定和有机物转化导致碳温室气体排放的证据。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-21 DOI: 10.1093/lambio/ovaf044

Andrew Elohim Laloo, Abhishek Gupta, Valeria Verrone, Rama Kant Dubey

{"title":"Role of Fe and Mn in organo-mineral-microbe interactions: evidence of carbon stabilization and transformation of organic matter leading to carbon greenhouse gas emissions.","authors":"Andrew Elohim Laloo, Abhishek Gupta, Valeria Verrone, Rama Kant Dubey","doi":"10.1093/lambio/ovaf044","DOIUrl":"https://doi.org/10.1093/lambio/ovaf044","url":null,"abstract":"Up to 90% of organic matter (OM) in soils and sediments are stabilized and protected against microbial decomposition through organo-mineral interactions, formation of soil aggregates, pH and oxygen availability. In soils and sediment systems OM is associated with mineral constituents promoting carbon persistence and sequestration of which iron (Fe) and manganese (Mn) are crucial components. Under anoxic condition, microbes couple the decomposition of OM to the oxidative/reductive transformation of Fe/Mn minerals leading to carbon greenhouse gas (C-GHG) emissions (i.e., CH4 and CO2). Although these organo-mineral-microbe interactions have been observed for decades, the bio-geochemical mechanisms governing the switch from OM stability toward OM degradation are not fully understood. Interest in this field have been growing steadily given the interest in global warming caused by OM decomposition leading to C-GHG emissions. This review emphasizes the dual role of Fe/Mn minerals in both OM stability and decomposition. Additionally, we synthesize the conceptual understanding of how Fe/Mn minerals govern OM dynamics and the resultant C-GHG emissions via microbial-mediated carbon transformation. We highlight the need for interdisciplinary research to better understand organo-Fe/Mn mineral-microbial interactions to develop management handles for climate mitigation strategies.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global epidemiology of high priority and pandemic Pseudomonas aeruginosa in pets, livestock, wild, and aquatic animals: a systematic review and meta-analysis. 宠物、家畜、野生动物和水生动物中高度优先和大流行的铜绿假单胞菌的全球流行病学：系统回顾和荟萃分析。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf028

Idris Nasir Abdullahi, Sirine Mejri, Chukwudi Cresent Okwume, Nurudeen Adedeji Lawal, Olatunde A Olusegun, Rym Ben Sallem, Karim Ben Slama

{"title":"Global epidemiology of high priority and pandemic Pseudomonas aeruginosa in pets, livestock, wild, and aquatic animals: a systematic review and meta-analysis.","authors":"Idris Nasir Abdullahi, Sirine Mejri, Chukwudi Cresent Okwume, Nurudeen Adedeji Lawal, Olatunde A Olusegun, Rym Ben Sallem, Karim Ben Slama","doi":"10.1093/lambio/ovaf028","DOIUrl":"10.1093/lambio/ovaf028","url":null,"abstract":"High-priority and international high-risk Pseudomonas aeruginosa (PA) are emergent strains. Here, we performed the first systematic review and meta-analysis of studies that reported the frequency of PA, carbapenem (CARBR), and colistin (COLR) resistant PA in healthy and sick livestock (A), pets (B), wild (C), and aquatic animals (D) from January 2013 to January 2023, globally. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, 57 cross-sectional and eight prospective studies were found eligible. Random-effect models were used to determine the pooled prevalences. Phylogenetic analyses based on core-genome single nucleotide polymorphisms (SNPs) were performed using CSI Phylogeny 1.4. The overall pooled prevalence of PA, COLR-PA, and CARBR-PA were 18.6%, 4%, and 11.7%, respectively. Wild animals had the highest pooled prevalence of PA, 33.5%. The pooled prevalence of COLR was significantly higher in PA strains from sick than healthy animals (P < 0.0001). Furthermore, COLR was significantly highest in PA strains from wild animals, 9.8% [95% confidence interval (CI): 0.8-58.9], while CARBR was highest in PA strains from pets, 14.8% (95% CI: 6.7-29.8). Seven (ST235, ST111, ST233, ST244, ST357, ST308, and ST277) of the 10 high-risk CARBR-PA clones carrying ExoS/U were reported in animals. The SNP-based phylogenetic analysis of 23 genomes of CARBR-PA from animals obtained from publicly available databases revealed two closely related blaGES-carrying strains (<100 SNPs) in sick pets, suggesting a transmission event. A higher frequency of high-risk carbapenem than colistin-resistant PA strains exists in animals. These findings highlight the roles of different animal species, especially pets and wild animals in the persistence and dissemination of high-priority PA.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Vibrio cholerae from the Jukskei River in Johannesburg, South Africa. 来自南非约翰内斯堡Jukskei河的霍乱弧菌的特征。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf036

Luyanda Mkhize, Musa Marimani, Sanelisiwe Thinasonke Duze

{"title":"Characterization of Vibrio cholerae from the Jukskei River in Johannesburg, South Africa.","authors":"Luyanda Mkhize, Musa Marimani, Sanelisiwe Thinasonke Duze","doi":"10.1093/lambio/ovaf036","DOIUrl":"10.1093/lambio/ovaf036","url":null,"abstract":"The current study aimed to isolate and characterize Vibrio cholerae isolated from the Jukskei River, one of the largest Rivers in Johannesburg, South Africa. Water samples collected from the Jukskei River were subjected to culture-based methods for the detection and isolation of V. cholerae. Twenty-four V. cholerae were isolated, confirmed using real-time PCR, and sequenced using the MInION portable nanopore-sequencing device. Reference-based genome assemblies were constructed from the raw reads using the EPI2ME software followed by bioinformatics analysis using the Centre for Genomic Epidemiology website. All the V. cholerae isolates isolated from the Jukskei River were classified as non-O1/non-O139 and none of the isolates harbored the cholera toxin gene, ctxA. All 24 V. cholerae isolates belonged to sequence type 741, virulent genes including toxR, vspD, als, hlyA, makA, and rtxA as well as the Vibrio pathogenicity island 2 were detected amongst the isolates. Antimicrobial resistance genes (parC, varG, and gyrA) were detected in 83% of isolates. Although V. cholerae non-O1/non-O139 are not associated with epidemic cholera they can still cause mild to life-threatening illnesses. Therefore, increased surveillance should be considered to better understand the public health risks to the local community.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling the effect of peracetic acid/hydrogen peroxide solution concentration and temperature treatment on Bacillus cereus spores inactivation. 模拟过氧乙酸/过氧化氢溶液浓度和温度对蜡样芽孢杆菌孢子灭活的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf033

Ivan Leguérinel, Anne-Gabrielle Mathot, Adrienne Camarero, Louis Coroller

{"title":"Modeling the effect of peracetic acid/hydrogen peroxide solution concentration and temperature treatment on Bacillus cereus spores inactivation.","authors":"Ivan Leguérinel, Anne-Gabrielle Mathot, Adrienne Camarero, Louis Coroller","doi":"10.1093/lambio/ovaf033","DOIUrl":"10.1093/lambio/ovaf033","url":null,"abstract":"Cleaning and sanitizing processing equipment are essential for achieving the hygiene standards required in food processing. The term \"sanitizing\" refers to the inactivation of microorganisms by disinfectants on previously cleaned surfaces and materials. In the food industry, commercial solutions of peracetic acid (PAA) and hydrogen peroxide (HP) show bactericidal and sporicidal activity at room temperature, and are used as disinfectants for food surfaces, fruit, and vegetables, or to sanitize water. The aim of this study was to quantify the impact of commercial solutions of PAA and HP on Bacillus cereus endospores as a function of concentration and temperature, in order to optimize its use. Inactivation kinetics were carried at 5 PAA/HP solution concentration levels and three temperature levels. Weibull model fits on concave inactivation kinetics quantified B. cereus spore resistances. A Bigelow-type model quantified the effects of concentration and temperature on spore resistance Weibull model parameters. The parameters of the Bigelow-type model enable to optimize disinfection treatments, reducing the concentration of PAA/HP solutions used by increasing the treatment temperature, while ensuring the same level of disinfectant efficacy against bacterial spores.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation potential of Pseudomonas aeruginosa to counteract Arsenite-induced phytotoxicity in Solanum lycopersicum cultivated within a contaminated agroecosystem. 铜绿假单胞菌对受污染农业生态系统栽培的番茄茄中砷酸盐诱导的植物毒性的生物修复潜力

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf027

Vivek Patel, Naga Rathna Supriya G

{"title":"Bioremediation potential of Pseudomonas aeruginosa to counteract Arsenite-induced phytotoxicity in Solanum lycopersicum cultivated within a contaminated agroecosystem.","authors":"Vivek Patel, Naga Rathna Supriya G","doi":"10.1093/lambio/ovaf027","DOIUrl":"10.1093/lambio/ovaf027","url":null,"abstract":"Trivalent arsenic (AsIII) is the most toxic form of arsenic, accumulates in plant systems through aquaporins, and inhibiting plant growth. This study focuses on mitigating the bioavailability of arsenite (AsIII) in agricultural soils through biological approaches. A potential AsIII tolerant bacterium Pseudomonas aeruginosa VS3 was isolated from contaminated soil. Subsequent analysis revealed that this strain can produce exopolysaccharides (EPS) and biofilms. Additionally, the strain exhibited production of plant growth promoting traits, incuding Indole-3-acetic acid (IAA), gibberellins, and silicon (Si) solubilisation. Biotransformation assay demonstrated that strain can oxidize AsIII to the less toxic arsenate (AsV) with conversion efficiency of 51%. Findings from the field trial proven that P. aeruginosa significantly reduced AsIII toxicity in Solanum lycopersicum and boosted plant growth under AsIII stress conditions. Additionally, inoculation with P. aeruginosa enhanced the activities of antioxidant enzymes (40% increase in peroxidase and a 17% increase in phenylalanine ammonia-lyase) compared to untreated controls under AsIII stress. The bacterial treatment reduced arsenic accumulation in root tissues, demonstrating P. aeruginosa VS3's potential as a bioremediation agent to alleviate arsenite stress and enhance plant growth.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial community transition in Surti buffalo-based fermented formulations sustainably enhances soil fertility and plant growth. 以苏尔特水牛为基础的发酵配方中微生物群落的转变可持续地提高了土壤肥力和植物生长。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf030

Komal Antaliya, Manoj Godhaniya, Janvi Galawala, Ashaka Vansia, Amit Mangrola, Anjana Ghelani, Rajesh Patel

{"title":"Microbial community transition in Surti buffalo-based fermented formulations sustainably enhances soil fertility and plant growth.","authors":"Komal Antaliya, Manoj Godhaniya, Janvi Galawala, Ashaka Vansia, Amit Mangrola, Anjana Ghelani, Rajesh Patel","doi":"10.1093/lambio/ovaf030","DOIUrl":"10.1093/lambio/ovaf030","url":null,"abstract":"This study investigates the role of microbial dynamics during the fermentation of buffalo dung and urine-fermented plant growth-promoting formulation, a natural biofertilizer, and its impact on plant growth and soil health. This formulation was prepared using Surti buffalo dung, urine, jaggery, gram flour, and soil and fermented for up to 14 days. Metagenomic analysis revealed microbial succession from a diverse initial community to a Bacillus-dominated population, especially the Lactic Acid Bacteria, after 8 days of fermentation. The changes were accompanied by increases in the plant growth-promoting genes related to nutrient acquisition, phytohormone production, and stress resistance. The pot experiment revealed a significant increase in mung bean growth, with the maximum effect obtained from the eighth-day fermented formulation. The experiment showed considerable improvement in the physicochemical properties of soil, including increased organic carbon and nutrient availability. These findings underscore the ecological importance of microbial input preparation in enhancing soil fertility and plant growth sustainably. Future research should delve deeper into the specific mechanisms these microbes facilitate nutrient cycling and resilience in various agroclimatic conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic nanovesicles encapsulating baicalin: a strategy to overcome colorectal cancer. 包裹黄芩苷的益生菌纳米颗粒：攻克结肠直肠癌的策略。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovae117

Yifan Li, Xinrui Zhao, Dan Wang, Ruilin Wang, Rui Zhu, Xiaojuan You, Xinwei Liu, Yongwei Li

{"title":"Probiotic nanovesicles encapsulating baicalin: a strategy to overcome colorectal cancer.","authors":"Yifan Li, Xinrui Zhao, Dan Wang, Ruilin Wang, Rui Zhu, Xiaojuan You, Xinwei Liu, Yongwei Li","doi":"10.1093/lambio/ovae117","DOIUrl":"10.1093/lambio/ovae117","url":null,"abstract":"Escherichia coli Nissle 1917 (EcN) is a unique probiotic utilized to treat inflammatory bowel disease and irritable bowel syndrome. Outer membrane vesicles (OMVs) from EcN can not only deliver pro-biotic anti-inflammatory molecules to cancer cells to exert anti-rectal cancer effects, but also deliver therapeutic drugs, genes, and other \"cargoes\" encapsulated in the vesicles to specific cells, making them an ideal vehicle for drug delivery. In this study, we successfully isolated OMVs of probiotic origin and obtained BCN-OMVs by encapsulating baicalin (BCN) in EcN-OMVs. Fluorescence microscopy showed that both fluorescently labeled EcN-OMVs and BCN-OMVs were able to enter HT-29 cells. The results of CCK-8, plate cloning, flow cytometry, and western blotting indicated that the vitality and proliferation of HT-29 cells were sensibly inhibited after treatment with BCN-OMVs. Additionally, apoptosis-related proteins and proteins involved in the NF-κB pathway were also expressed differently. The results above suggest that EcN-OMVs, serving as a bio-nanocarrier of BCN, can effectively address the limitations of BCN, such as poor water solubility and low bioavailability. They also play a significant anti-tumor role by enhancing cancer cell apoptosis, showing promising potential in targeted therapy for colorectal cancer (CRC).","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0