Letters in Applied Microbiology最新文献_第2页

Editor's note on 'Preventing contamination of PCR-based multiplex assays including the use of a dedicated biosafety cabinet'. 关于 "防止基于 PCR 的多重检测污染，包括使用专用生物安全柜 "的编者按。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae096

引用次数: 0

Antibacterial effect of Cu2O/TiO2 visible-light photocatalytic composite on Xanthomonas campestris. Cu2O/TiO2 可见光光催化复合材料对野菜黄单胞菌的抗菌作用

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae087

Ying Jiang, Shiyu Zhou, Liuhong Chen, Yuning Huo, Guozheng Huang, Jianguo Cao, Xiling Dai

{"title":"Antibacterial effect of Cu2O/TiO2 visible-light photocatalytic composite on Xanthomonas campestris.","authors":"Ying Jiang, Shiyu Zhou, Liuhong Chen, Yuning Huo, Guozheng Huang, Jianguo Cao, Xiling Dai","doi":"10.1093/lambio/ovae087","DOIUrl":"10.1093/lambio/ovae087","url":null,"abstract":"In this study, a Cu2O/TiO2 (CuTi) visible-light photocatalytic composite was employed for the treatment of Xanthomonas campestris and X. campestris-infected Brassica napus seedlings. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against X. campestris were determined to be 8 and 32 μg ml-1, respectively. Transmission electron microscopy analysis demonstrated a direct correlation between the extent of bacterial cell damage and the concentration of CuTi. Noteworthily, a bactericidal rate of 100% was achieved at a concentration of 150 μg ml-1 over a treatment duration of 120 min. Moreover, alterations in active oxidants and antioxidants, including reactive oxygen species, glutathione reductase, superoxide dismutase, peroxidase, and catalase within the bacterial cells, were examined to elucidate the underlying mechanism of inhibition by the CuTi. The B. napus infected by X. campestris was treated with CuTi, and the efficacy was validated through determination of plant resistance indexes. The combined data confirmed that the CuTi is characterized by a low dose, fast onset, good effect, and higher safety for killing X. campestris, and it is expected to be developed as an antimicrobial agent for vegetables.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of colistin-resistant carbapenemase producing Klebsiella pneumoniae in a river receiving wastewater treatment plant effluent. 接收污水处理厂污水的河流中产生耐大肠菌素碳青霉烯酶肺炎克雷伯氏菌的特征。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae090

Pragyan Paramita Swain, Saubhagini Sahoo, Birasen Behera, Dibyajyoti Uttameswar Behera, Enketeswara Subudhi, Rajesh Kumar Sahoo

{"title":"Characterization of colistin-resistant carbapenemase producing Klebsiella pneumoniae in a river receiving wastewater treatment plant effluent.","authors":"Pragyan Paramita Swain, Saubhagini Sahoo, Birasen Behera, Dibyajyoti Uttameswar Behera, Enketeswara Subudhi, Rajesh Kumar Sahoo","doi":"10.1093/lambio/ovae090","DOIUrl":"10.1093/lambio/ovae090","url":null,"abstract":"Genes conferring antibiotic resistance phenotype, particularly to last resort antibiotics, pose a significant concern globally. Wastewater treatment plant (WWTP) effluent substantially contributes to antibiotic resistance in receiving rivers, threatening human health. Globally, colistin- and carbapenem-resistant Klebsiella pneumoniae infections cause high morbidity and mortality. We investigated colistin-resistant carbapenemase-producing K. pneumoniae (Co-CRKP) isolates in Kathajodi river receiving WWTP effluent, their resistance genes, and pathogenic potential. Four isolates (Co-CRKP-7, Co-CRKP-8, Co-CRKP-10, and Co-CRKP-15) exhibited extensively drug-resistant (XDR) phenotype, harbouring blaTEM-1, blaCTX-M-15, blaNDM-5, and blaOXA-48 genes. Colistin resistance was attributed to mutations in the pmrA and pmrB genes. Virulence genes (fimH, mrkD, entB, iucA, iutA, and irp1), capsular serotypes (K1, K2) and biofilm formation in the isolates explicated their pathogenicity. Furthermore, Inc plasmid replicons (Y, FrepB, P, K/B, L/M, N, FIA, A/C, and FIB) indicated the dissemination potential of the resistance genes in Co-CRKP isolates. The multi-locus sequence typing showed that Co-CRKP-7 and Co-CRKP-8 belonged to ST42, while Co-CRKP-10 and Co-CRKP-15 were ST16 and ST231, respectively. These high-risk clones carrying multidrug resistance and virulence genes, implicated in numerous outbreaks, have spread worldwide. Our findings emphasize the necessity for effective treatment of hospital wastes to restrict the spread of clinical isolates into aquatic environments.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A One Health exploration of antimicrobial resistance in Escherichia coli originated from urban and rural lakes ecosystem. 源自城市和农村湖泊生态系统的大肠杆菌抗菌药耐药性的综合健康探索。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae095

Priyanka Priyanka, Prem Raj Meena, Dharma Raj, Purnima Mishra, Anand Kumar Jha, K Siddaardha Duggirala, Akshay Dhanokar, Amit Kumar, Anuj Rana, Arvind Pratap Singh

{"title":"A One Health exploration of antimicrobial resistance in Escherichia coli originated from urban and rural lakes ecosystem.","authors":"Priyanka Priyanka, Prem Raj Meena, Dharma Raj, Purnima Mishra, Anand Kumar Jha, K Siddaardha Duggirala, Akshay Dhanokar, Amit Kumar, Anuj Rana, Arvind Pratap Singh","doi":"10.1093/lambio/ovae095","DOIUrl":"10.1093/lambio/ovae095","url":null,"abstract":"Antimicrobial resistance (AMR) has become one of the most serious threats to One Health. Aquatic environments are an ideal non-clinical AMR reservoir and can act as a key battlefront for tackling the AMR. However, AMR data using the One Health approach remain scarce in aquatic environments worldwide. Here, we extensively assessed AMR in Escherichia coli isolated from urban and rural lake ecosystems using the One Health perspective. A total of 162 E. coli isolates obtained from lakes were tested against 25 antimicrobials using an in-vitro antimicrobial susceptibility testing method. A low (2%) to moderate (45%) drug resistance rate was found for all antimicrobials used in human/veterinary medicine or animal/plant agriculture. However, <80% E. coli isolates exhibited multidrug resistance (MDR) phenotype to highly important (amikacin, gentamicin, trimethoprim) or critically important (amoxicillin, ampicillin, colistin) drugs of both human and veterinary medicine. Of concern, >50% of E. coli isolates exhibited MDR to drugs used as last-resorts (chloramphenicol, colistin) or as frontline (nitrofurantoin, sulfamethoxazole, ampicillin, gentamicin) against E. coli infections. In conclusion, the presence of MDR E. coli strains in urban or rural lake ecosystems highlights their possible role as AMR reservoirs with potential One Health risks.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and molecular characterization of vancomycin resistant enterococci from wild birds: first detection of a plasmid-borne vanC1 in Enterococcus faecalis. 野生鸟类中抗万古霉素肠球菌的表型和分子特征：首次在粪肠球菌中检测到质粒携带的 vanC1。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae098

Yousra Hachem, Lydia Neila Djouadi, Anis Raddaoui, Fella Boukli-Hacene, Hanane Boumerdassi, Wafa Achour, Farida Nateche

{"title":"Phenotypic and molecular characterization of vancomycin resistant enterococci from wild birds: first detection of a plasmid-borne vanC1 in Enterococcus faecalis.","authors":"Yousra Hachem, Lydia Neila Djouadi, Anis Raddaoui, Fella Boukli-Hacene, Hanane Boumerdassi, Wafa Achour, Farida Nateche","doi":"10.1093/lambio/ovae098","DOIUrl":"https://doi.org/10.1093/lambio/ovae098","url":null,"abstract":"Vancomycin-resistant enterococci (VRE) are a public health concern as they lead to therapeutic impasses and play a pivotal role in the dissemination of vancomycin resistance genes. As recent evidence suggests that wildlife can play a role in the dissemination of bacterial resistomes, this study explored the potential role of Algerian wild birds as a reservoir of VRE. A total of 222 cloacal and fecal samples were collected from various wild bird species and screened for VRE using a selective medium. Of the 47 isolated strains, 22 were identified as Enterococcus casseliflavus with the vanC2/C3 gene, 24 as Enterococcus gallinarum (19 carrying vanC1 and five carrying vanC2/C3), and one strain as Enterococcus faecalis with the vanC1 gene. Twenty-four (24) strains were multidrug-resistant with 61.7% resistant to rifampicin, while no resistance to teicoplanin, linezolid, and gentamicin was found. Additionally, 53.20% of the strains exhibited at least one virulence factor. To our knowledge, this study represents the first documentation of the vanC1 gene in E. faecalis isolated from wild birds. Furthermore, this gene was found to be carried by a conjugative plasmid, highlighting its ability to spread among bacterial populations and lead to the emergence of novel resistance phenotypes.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the medium composition for the production of the natural blue-violet pigment violacein by a new Janthinobacterium sp. isolate. 改进培养基成分，利用新分离的 Janthinobacterium sp.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae091

Caroline Kie Ishimoto, Rodolfo Dantas Lima Junior, Simone Aparecida de Lima Scaramussa, Taicia Pacheco Fill, Valéria Maia Oliveira, Juliano Lemos Bicas

{"title":"Improving the medium composition for the production of the natural blue-violet pigment violacein by a new Janthinobacterium sp. isolate.","authors":"Caroline Kie Ishimoto, Rodolfo Dantas Lima Junior, Simone Aparecida de Lima Scaramussa, Taicia Pacheco Fill, Valéria Maia Oliveira, Juliano Lemos Bicas","doi":"10.1093/lambio/ovae091","DOIUrl":"10.1093/lambio/ovae091","url":null,"abstract":"The interest in natural compounds has increased primarily due to their beneficial health and environmental aspects. However, natural sources of some compounds, such as bluish pigments, are limited, requiring the development of efficient processes to meet commercial demands. This study isolated a blue-violet bacterium from spoiled cooked rice and identified it as a potential new species of Janthinobacterium through 16S rDNA analysis. Ultra-high performance liquid chromatography-tandem mass spectrometry analyses confirmed that the blue-violet pigment violacein was responsible for the bluish color. In laboratory conditions, different carbon and nitrogen sources were evaluated in submerged culture media to enhance pigment production. Glycerol did not result in significant pigment production by this strain, as expected from previous reports. Instead, a culture medium composed of yeast extract and fructose yielded higher pigment production, reaching about 113.68 ± 16.68 mg l-1 after 120 h. This result provides crucial insights for future studies aiming for sustainable and commercially viable violacein production. Based on a bioeconomy concept, this approach has the potential to supply natural and economic bluish pigments for various industrial sectors, including pharmaceutical, cosmetic, and food.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More than just the gene: investigating expression using a non-native plasmid and host and its impact on resistance conferred by β-lactamase OXA-58 isolated from a hospital wastewater microbiome. 不仅仅是基因：研究使用非本地质粒和宿主的表达及其对从医院废水微生物组中分离出的β-内酰胺酶 OXA-58 所产生的抗药性的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2024-10-01 DOI: 10.1093/lambio/ovae097

J Wu, T P Thompson, N H O'Connell, K McCracken, J Powell, B F Gilmore, C P Dunne, S A Kelly

{"title":"More than just the gene: investigating expression using a non-native plasmid and host and its impact on resistance conferred by β-lactamase OXA-58 isolated from a hospital wastewater microbiome.","authors":"J Wu, T P Thompson, N H O'Connell, K McCracken, J Powell, B F Gilmore, C P Dunne, S A Kelly","doi":"10.1093/lambio/ovae097","DOIUrl":"10.1093/lambio/ovae097","url":null,"abstract":"With the escalation of hospital-acquired infections by multidrug resistant bacteria, understanding antibiotic resistance is of paramount importance. This study focuses on the β-lactamase gene, blaOXA-58, an important resistance determinant identified in a patient-facing hospital wastewater system. This study aimed to characterize the behaviour of the OXA-58 enzyme when expressed using a non-native plasmid and expression host. blaOXA-58 was cloned using a pET28a(+)/Escherichia coli BL21(DE3) expression system. Nitrocefin hydrolysis and antimicrobial susceptibility of OXA-58-producing cells were assessed against penicillin G, ampicillin, meropenem, and amoxicillin. blaOXA-58 conferred resistance to amoxicillin, penicillin G, and ampicillin, but not to meropenem. This was unexpected given OXA-58's annotation as a carbapenemase. The presence of meropenem also reduced nitrocefin hydrolysis, suggesting it acts as a competitive inhibitor of the OXA-58 enzyme. This study elucidates the phenotypic resistance conferred by an antimicrobial resistance gene (ARG) obtained from a clinically relevant setting and reveals that successful functional expression of ARGs is multifaceted. This study challenges the reliability of predicting antimicrobial resistance based solely on gene sequence alone, and serves as a reminder of the intricate interplay between genetics and structural factors in understanding resistance profiles across different host environments.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of surface swab with optical microscopy for detection and quantification of bacterial cells from stainless-steel surfaces. 将表面拭子与光学显微镜相结合，用于检测和量化不锈钢表面的细菌细胞。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-09-17 DOI: 10.1093/lambio/ovae089

Yuzhen Zhang,Zili Gao,Lili He

{"title":"Integration of surface swab with optical microscopy for detection and quantification of bacterial cells from stainless-steel surfaces.","authors":"Yuzhen Zhang,Zili Gao,Lili He","doi":"10.1093/lambio/ovae089","DOIUrl":"https://doi.org/10.1093/lambio/ovae089","url":null,"abstract":"Swab sampling is a common method for recovering microbes on various environmental surfaces. Its successful application for a specific target depends on the proper swab method and the following detection assay. Herein, we evaluated critical factors influencing surface swab sampling, aiming to achieve the optimal detection and quantification performance of optical detection for bacterial cells on stainless-steel surfaces. Our results showed the recovery rate of Salmonella enterica (SE1045) cells from the 10×10 cm2 stainless-steel surface reached up to 92.71±2.19% when using ammonia bicarbonate-moistened polyurethane foam swabs for gentle collection, followed by ultrasound-assisted release in NH4HCO3 solution. Among the six different foam swabs, the Puritan™ Sterile Large Foam Swab contributed the lowest background noise and highest recovery efficiency when integrated with the optical detection assay. Notably, our method exhibited a strong linear relationship (r2 = 0.9983) between the detected cell numbers and the theoretical number of SE1045 cells seeded on surfaces in the range of 104-107 CFU, with a limit of detection of 7.2×104 CFU 100 cm-2. This integration was completed within 2 hours, exhibiting the applicable potential in various settings.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro efficacy of aquaculture antimicrobials and genetic determinants of resistance in bacterial isolates from tropical aquaculture disease outbreaks. 水产养殖抗菌剂的体外药效和热带水产养殖疾病爆发中细菌分离物的抗药性遗传决定因素。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-09-13 DOI: 10.1093/lambio/ovae088

Sumithra T Gopakumar,KrupeshaSharma S Ramachandra,Suja Gangadharan,Anusree V Nair,Surya S Gayathri,Vishnu Prasad,Lailaja V Purakal,George J Chakkalakkal,Prasanna K Patil

{"title":"In vitro efficacy of aquaculture antimicrobials and genetic determinants of resistance in bacterial isolates from tropical aquaculture disease outbreaks.","authors":"Sumithra T Gopakumar,KrupeshaSharma S Ramachandra,Suja Gangadharan,Anusree V Nair,Surya S Gayathri,Vishnu Prasad,Lailaja V Purakal,George J Chakkalakkal,Prasanna K Patil","doi":"10.1093/lambio/ovae088","DOIUrl":"https://doi.org/10.1093/lambio/ovae088","url":null,"abstract":"Understanding the efficacy of antimicrobials against pathogens from clinical samples is critical for their responsible use. The manuscript presents in vitro efficacy and antimicrobial resistance (AMR) genes in seven species of fish pathogens from the disease outbreaks of Indian aquaculture against oxytetracycline, florfenicol, oxolinic acid, and enrofloxacin. In vitro efficacy was evaluated by minimum inhibitory concentration and minimum bactericidal concentration. The gene-specific PCR screened AMR genes against quinolones (qnrA, qnrB and qnrS) and tetracyclines (tetM, tetS, tetA, tetC, tetB, tetD, tetE, tetH, tetJ, tetG, and tetY). The results showed that Aeromonas veronii (45%) showed the maximum resistance phenotype followed by Streptococcus agalactiae (40%), Photobacterium damselae (15%), Vibrio parahaemolyticus (10%), and Vibrio vulnificus (5%). There was no resistance among Vibrio harveyi and Vibrio alginolyticus against the tested antimicrobials. The positive association between tetA, tetB, tetC, tetM, or a combination of these genes to oxytetracycline resistance and qnrS to quinolone resistance indicated their potential in surveillance studies. The prevalence of resistance phenotypes (16.43%) and evaluated AMR genes (2.65%) against aquaculture antimicrobials was low. The resistance phenotype pattern abundance was 0.143. All the isolates showed susceptibility to florfenicol. The results help with the appropriate drug selection against each species in aquaculture practices.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial potential of Streptomyces sp. NP73 isolated from the forest soil of Northeast India against multi-drug resistant Escherichia coli. 从印度东北部森林土壤中分离出的链霉菌 NP73 对耐多药大肠杆菌的抗菌潜力。

IF 2.4 4区生物学

Letters in Applied Microbiology Pub Date : 2024-09-11 DOI: 10.1093/lambio/ovae086

Aditya Narayan Konwar,Surajit Basak,Kangkon Saikia,Shalini Gurumayum,Nitya Panthi,Jagat Chandra Borah,Debajit Thakur

{"title":"Antimicrobial potential of Streptomyces sp. NP73 isolated from the forest soil of Northeast India against multi-drug resistant Escherichia coli.","authors":"Aditya Narayan Konwar,Surajit Basak,Kangkon Saikia,Shalini Gurumayum,Nitya Panthi,Jagat Chandra Borah,Debajit Thakur","doi":"10.1093/lambio/ovae086","DOIUrl":"https://doi.org/10.1093/lambio/ovae086","url":null,"abstract":"This study reports the isolation and characterization of a Streptomyces sp. from soil, capable of producing bioactive secondary metabolites active against a variety of bacterial human pathogens. We targeted the antimicrobial activity against Escherichia coli ATCC-BAA 2469, a clinically relevant strain of bacteria harbouring resistance genes for carbapenems, extended spectrum beta-lactams, tetracyclines, fluoroquinones, etc. Preliminary screening using the spot inoculation technique identified Streptomyces sp. NP73 as the potent strain among the 74 isolated Actinomycetia strain. 16S rRNA gene and whole genome sequencing (WGS) confirmed its taxonomical identity and helped in the construction of the phylogenetic tree. WGS revealed the predicted pathways and biosynthetic gene clusters responsible for producing various types of antibiotics including the isolated compound. Bioactivity guided fractionation and chemical characterization of the active fraction, carried out using liquid chromatography, Gas chromatography-mass spectrometry, Infra-red spectroscopy, and Nuclear magnetic resonance spectroscopy, led to the tentative identification of the active compound as Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-, a diketopiperazine molecule. This compound exhibited excellent antimicrobial and anti-biofilm properties against Escherichia coli ATCC-BAA 2469 with an MIC value of 15.64 µg mL-1, and the low cytotoxicity of the compound identified in this study provides hope for future drug development.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0