Letters in Applied Microbiology最新文献_第2页

Bioremediation potential of Pseudomonas aeruginosa to counteract Arsenite-induced phytotoxicity in Solanum lycopersicum cultivated within a contaminated agroecosystem.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf027

Vivek Patel, Naga Rathna Supriya G

{"title":"Bioremediation potential of Pseudomonas aeruginosa to counteract Arsenite-induced phytotoxicity in Solanum lycopersicum cultivated within a contaminated agroecosystem.","authors":"Vivek Patel, Naga Rathna Supriya G","doi":"10.1093/lambio/ovaf027","DOIUrl":"10.1093/lambio/ovaf027","url":null,"abstract":"Trivalent arsenic (AsIII) is the most toxic form of arsenic, accumulates in plant systems through aquaporins, and inhibiting plant growth. This study focuses on mitigating the bioavailability of arsenite (AsIII) in agricultural soils through biological approaches. A potential AsIII tolerant bacterium Pseudomonas aeruginosa VS3 was isolated from contaminated soil. Subsequent analysis revealed that this strain can produce exopolysaccharides (EPS) and biofilms. Additionally, the strain exhibited production of plant growth promoting traits, incuding Indole-3-acetic acid (IAA), gibberellins, and silicon (Si) solubilisation. Biotransformation assay demonstrated that strain can oxidize AsIII to the less toxic arsenate (AsV) with conversion efficiency of 51%. Findings from the field trial proven that P. aeruginosa significantly reduced AsIII toxicity in Solanum lycopersicum and boosted plant growth under AsIII stress conditions. Additionally, inoculation with P. aeruginosa enhanced the activities of antioxidant enzymes (40% increase in peroxidase and a 17% increase in phenylalanine ammonia-lyase) compared to untreated controls under AsIII stress. The bacterial treatment reduced arsenic accumulation in root tissues, demonstrating P. aeruginosa VS3's potential as a bioremediation agent to alleviate arsenite stress and enhance plant growth.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial community transition in Surti buffalo-based fermented formulations sustainably enhances soil fertility and plant growth.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf030

Komal Antaliya, Manoj Godhaniya, Janvi Galawala, Ashaka Vansia, Amit Mangrola, Anjana Ghelani, Rajesh Patel

{"title":"Microbial community transition in Surti buffalo-based fermented formulations sustainably enhances soil fertility and plant growth.","authors":"Komal Antaliya, Manoj Godhaniya, Janvi Galawala, Ashaka Vansia, Amit Mangrola, Anjana Ghelani, Rajesh Patel","doi":"10.1093/lambio/ovaf030","DOIUrl":"10.1093/lambio/ovaf030","url":null,"abstract":"This study investigates the role of microbial dynamics during the fermentation of buffalo dung and urine-fermented plant growth-promoting formulation, a natural biofertilizer, and its impact on plant growth and soil health. This formulation was prepared using Surti buffalo dung, urine, jaggery, gram flour, and soil and fermented for up to 14 days. Metagenomic analysis revealed microbial succession from a diverse initial community to a Bacillus-dominated population, especially the Lactic Acid Bacteria, after 8 days of fermentation. The changes were accompanied by increases in the plant growth-promoting genes related to nutrient acquisition, phytohormone production, and stress resistance. The pot experiment revealed a significant increase in mung bean growth, with the maximum effect obtained from the eighth-day fermented formulation. The experiment showed considerable improvement in the physicochemical properties of soil, including increased organic carbon and nutrient availability. These findings underscore the ecological importance of microbial input preparation in enhancing soil fertility and plant growth sustainably. Future research should delve deeper into the specific mechanisms these microbes facilitate nutrient cycling and resilience in various agroclimatic conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the anti-obesity effects of Lactobacillus in C57BL/6 mice: mechanisms, interventions, and future directions.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf024

Sharoen Yu Ming Lim, E-Jayn Chong, Weng Yan Mah, Yan Pan, Chee Mun Fang, Chandrika Murugaiah, Mustafa Alshagga

{"title":"Exploring the anti-obesity effects of Lactobacillus in C57BL/6 mice: mechanisms, interventions, and future directions.","authors":"Sharoen Yu Ming Lim, E-Jayn Chong, Weng Yan Mah, Yan Pan, Chee Mun Fang, Chandrika Murugaiah, Mustafa Alshagga","doi":"10.1093/lambio/ovaf024","DOIUrl":"10.1093/lambio/ovaf024","url":null,"abstract":"Lactobacillus species show strong potential in fighting obesity-related inflammation and metabolic issues. Obesity causes inflammation in adipose tissue, which harms insulin sensitivity and leads to fat buildup. Lactobacillus strains like Lactobacillus gasseri, Lactobacillus reuteri, and Lactobacillus plantarum help regulate lipid metabolism by boosting key genes, preventing fat cell formation, and encouraging fat breakdown. They also produce short-chain fatty acids (SCFAs) that improve gut health, activate metabolic pathways, and reduce inflammation. Studies in animals have shown that Lactobacillus can reduce body weight, fat, and inflammation, with Lactobacillus plantarum being especially effective in improving gut microbiota and liver function. When combined with other probiotics or prebiotics, these strains work even better, enhancing lipid metabolism and reducing inflammation. These results suggest that Lactobacillus could be an effective way to manage obesity and related health problems by influencing metabolism, gut health, and inflammation. However, more research, particularly human clinical trials, is needed to confirm its potential as a dietary treatment for obesity.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress resistance of the biofilm and planktonic forms of Lactobacillus delbrueckii in adverse environments.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf031

Jiangyang Zhai, Lili Nie, Jianjun Tian, Yinfeng He, Yue Gu

{"title":"Stress resistance of the biofilm and planktonic forms of Lactobacillus delbrueckii in adverse environments.","authors":"Jiangyang Zhai, Lili Nie, Jianjun Tian, Yinfeng He, Yue Gu","doi":"10.1093/lambio/ovaf031","DOIUrl":"10.1093/lambio/ovaf031","url":null,"abstract":"Biofilm and planktonic forms are different kinds of self-protection mechanisms in microorganisms for resistance to adverse environments. The research explored the physicochemical properties, antimicrobial, and antioxidant activities of Lactobacillus delbrueckii (L. delbrueckii) 5E, focusing on the biofilm's stress tolerance. L. delbrueckii 5E's high lactic acid production (709.1 g·L-1) enhances its antimicrobial activity, which is pH-dependent and decreases when the supernatant is adjusted to pH 6.5. The cell-free fermentation supernatant, bacterial suspension, and cell-free extract of L. delbrueckii 5E showed significant scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion radicals. L. delbrueckii 5E formed biofilms with a bacterial count of 9.00 × 106 CFU·mL-1 on polyacrylonitrile electrospun membranes. Stress-tolerance tests indicated that the biofilm form of L. delbrueckii 5E exhibited superior survival under high temperatures, osmotic pressures, bile salts, potassium sorbate, and H2O2 exposure. The biofilm consistently released free bacteria, maintaining a stable total colony count of 106 CFU·mL-1. The remarkable antibacterial and antioxidant properties of L. delbrueckii 5E, along with its resilience to harsh environments, establish its potential for applications in the food industry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beltraniella portoricensis strain MRH42, a saprophytic fungus from Mexico, produces antimicrobial and cytotoxic hymenopsins.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf023

Ana L Ruiz-Jiménez, Gabriela Heredia, Karla Peraza-Jiménez, Felicia Amalia Moo-Koh, Francisca Vicente, Mercedes de la Cruz, Nuria de Pedro, Jesús Martín, Fernando Reyes, Marcela Gamboa-Angulo

引用次数: 0

1,3,4-oxadiazoles with effective anti-mycobacterial activity.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf029

Patrícia de Mattos Andriato, Vanessa Pietrowski Baldin, Aryadne Larissa de Almeida, Eloisa Gibin Sampiron, Sandra Sayuri Nakamura de Vasconcelos, Katiany Rizzieri Caleffi-Fercioli, Regiane Bertin de Lima Scodro, Jean Eduardo Meneguello, Bernard Maigret, Érika Seki Kioshima, Rosilene Fressatti Cardoso

{"title":"1,3,4-oxadiazoles with effective anti-mycobacterial activity.","authors":"Patrícia de Mattos Andriato, Vanessa Pietrowski Baldin, Aryadne Larissa de Almeida, Eloisa Gibin Sampiron, Sandra Sayuri Nakamura de Vasconcelos, Katiany Rizzieri Caleffi-Fercioli, Regiane Bertin de Lima Scodro, Jean Eduardo Meneguello, Bernard Maigret, Érika Seki Kioshima, Rosilene Fressatti Cardoso","doi":"10.1093/lambio/ovaf029","DOIUrl":"10.1093/lambio/ovaf029","url":null,"abstract":"The search for new drugs to treat tuberculosis and nontuberculous mycobacteria (NTM)-caused diseases is still desired. This is the first study aimed at determining the activity of two innovative synthetic 1,3,4-oxadiazole molecules, (4-[cyclohexyl(ethyl) sulfamoyl]-N-[5-(furan-2-yl)-1,3,4-oxadiazol-2-yl]benzamide), namely LMM11, and ((N-cyclo-hexyl-N-ethylsulfamoil)-N-(5- (4-fluorophenyl)-1,3,4-oxadiazol-2-il) benzamide), namely LMM6, against Mycobacterium tuberculosis and nontuberculous mycobacteria, and their ability to present synergism in activity against M. tuberculosis when combined with anti-TB drugs. In vitro cytotoxicity studies were conducted in HeLa and VERO cells. The minimum inhibitory concentration (MIC) and combinatory effect were carried out in M. tuberculosis H37Rv and resistant isolates, NTM, and other genera of bacteria. The LMM6 and LMM11 MIC ranged from 8.27 to 33.07 µM and 15.58 to 70.30 µM in M. tuberculosis, respectively. LMM6 showed activity against M. smegmatis mc2 155 (8.25 μM), M. szulgai (2.05 μM), and M. kansasii (66.03 μM), while LMM11 showed activity against M. szulgai (8.77 μM), and M. smegmatis (70.19 μM). Synergism and modulatory activity of LMM6 and LMM11 with anti-TB drugs were observed, and they showed to be more selective for mycobacteria than HeLa and VERO cells. Both new oxadiazoles showed activity against mycobacteria, in fact, more pronounced against M. tuberculosis, and seem to bring light to the synthesis of new antimicobacterial.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ability of Salmonella enterica subsp. enterica strains to form biofilms on abiotic surfaces and their susceptibility to selected essential oil components. 肠炎沙门氏菌亚种在非生物表面形成生物膜的能力及其对精油成分的敏感性。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf032

Liliana Pérez-Lavalle, Anabela Borges, Inês B Gomes, Elena Carrasco, Antonio Valero, Manuel Simões

{"title":"The ability of Salmonella enterica subsp. enterica strains to form biofilms on abiotic surfaces and their susceptibility to selected essential oil components.","authors":"Liliana Pérez-Lavalle, Anabela Borges, Inês B Gomes, Elena Carrasco, Antonio Valero, Manuel Simões","doi":"10.1093/lambio/ovaf032","DOIUrl":"10.1093/lambio/ovaf032","url":null,"abstract":"The ability of Salmonella enterica subsp. enterica to persist and form biofilms on different surfaces can constitute a source of food contamination, being an issue of global concern. The objective of this study was to understand the biofilm formation profile of 14 S. enterica strains among different serovars and sources and to evaluate the ability of essential oil (EO) components (carveol, citronellol, and citronellal) to disinfect the biofilms formed on stainless steel and polypropylene surfaces. All the strains were able to form biofilms with counts between 5.34 to 6.78 log CFU cm-2. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EO components were evaluated on two selected strains. All compounds inhibited the growth of Salmonella Typhimurium (strain 1; MIC = 800-1000 µg ml-1) and Salmonella Enteritidis (strain 5; MIC = 400-1000 µg ml-1) and only carveol showed bactericidal activity against strains 1 and 5 (MBC = 1200 µg ml-1). Biofilms were exposed to the EO components at 10 × MIC for 30 min and polypropylene surfaces were more difficult to disinfect showing reductions between 0.9 and <1.2 log CFU cm-2. In general, the S. enterica biofilms demonstrated a significant tolerance to disinfection, demonstrating their high degree of recalcitrance on food processing surfaces.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelic exchange technologies for Clostridioides difficile research and their application in toxin studies. 用于艰难梭菌研究的等位基因交换技术及其在毒素研究中的应用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf034

Terry W Bilverstone

引用次数: 0

Clonal expression and structural analysis of polylactic acid-degrading enzyme S8SP from Bacillus safensis.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf025

Yujun Wang, Yuanyi Zhang, Chunwang Li, Siqi Meng, Dandan Wang, Lehui Zhao, Zhanyong Wang

{"title":"Clonal expression and structural analysis of polylactic acid-degrading enzyme S8SP from Bacillus safensis.","authors":"Yujun Wang, Yuanyi Zhang, Chunwang Li, Siqi Meng, Dandan Wang, Lehui Zhao, Zhanyong Wang","doi":"10.1093/lambio/ovaf025","DOIUrl":"10.1093/lambio/ovaf025","url":null,"abstract":"Polylactic acid (PLA) is one of the most popular biodegradable plastics favored over traditional plastics. However, it is more difficult to degrade than other biodegradable plastics probably due to the low species and number of PLA-degrading microorganisms degrading enzymes in the environment. Therefore, identifying PLA-degrading microorganisms and enzymes is of great significance for the popularization and application of PLA. This study identified a PLA-degrading enzyme, S8 serine peptidase (S8SP), from Bacillus safensis, and the heterologous expression of S8SP was conducted in Escherichia coli. PLA degradation ability of S8SP was investigated using scanning electron microscopy and water contact angle. The surface of S8SP-degraded PLA films showed obvious cracks and pits and exhibited improved hydrophilicity. The molecular weight of S8SP was about 42 kDa, and its optimum temperature and pH were 40°C and 8.0, respectively. S8SP could maintain high stability in the temperature range of 30°C-40°C and pH range of 7.0-9.0. Sodium ions (Na+), potassium ions (K+), Triton X-100, and Tween-80 promoted the enzyme activity of S8SP. S8SP had a high similarity degree to S8 serine peptidase from the genus Bacillus, and had the classical hydrolase-catalyzed triplet structure.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Learn from artificial intelligence: the pursuit of objectivity.

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-03-03 DOI: 10.1093/lambio/ovaf021

Fengyi Wang, Angeliki Marouli, Pisit Charoenwongwatthana, Chien-Yi Chang

{"title":"Learn from artificial intelligence: the pursuit of objectivity.","authors":"Fengyi Wang, Angeliki Marouli, Pisit Charoenwongwatthana, Chien-Yi Chang","doi":"10.1093/lambio/ovaf021","DOIUrl":"10.1093/lambio/ovaf021","url":null,"abstract":"Humans continuously face threats from emerging novel pathogens and antimicrobial resistant bacteria or fungi, which requires urgently and efficient solutions. Alternatively, microbes also produce compounds or chemicals highly valuable to humans of which require continuous refinement and improvement of yields. Artificial intelligence (AI) is a promising tool to search for solutions combatting against diseases and facilitating productivity underpinned by robust research providing accurate information. However, the extent of AI credibility is yet to be fully understood. In terms of human bias, AI could arguably act as a means of ensuring scientific objectivity to increase accuracy and precision, however, whether this is possible or not has not been fully discussed. Human bias and error can be introduced at any step of the research process, including conducting experiments and data processing, through to influencing clinical applications. Despite AI's contribution to advancing knowledge, the question remains, is AI able to achieve objectivity in microbiological research? Here, the benefits, drawbacks, and responsibilities of AI utilization in microbiological research and clinical applications were discussed.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0