Letters in Applied Microbiology最新文献_第2页

Arbuscular mycorrhizal fungal symbiosis in New Zealand ecosystems: challenges and opportunities. 新西兰生态系统中的丛枝菌根真菌共生：挑战和机遇。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-09 DOI: 10.1093/lambio/ovaf070

Romy Moukarzel, Lauren P Waller, E Eirian Jones, Hayley J Ridgway

{"title":"Arbuscular mycorrhizal fungal symbiosis in New Zealand ecosystems: challenges and opportunities.","authors":"Romy Moukarzel, Lauren P Waller, E Eirian Jones, Hayley J Ridgway","doi":"10.1093/lambio/ovaf070","DOIUrl":"10.1093/lambio/ovaf070","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs that form a symbiotic and mutualistic relationship with most terrestrial plants, playing an important role in plant growth, nutrient acquisition, and ecosystem stability. This review synthesizes current knowledge on AMF colonization in plants within New Zealand ecosystems, including the challenges and opportunities of molecular identification techniques used in characterizing AMF communities in natural and managed systems. The ecosystem services provided by AMF, such as improved growth parameters, enhanced nutrition, and disease control, are discussed in detail, highlighting their significance in sustainable agriculture and natural ecosystems. Additionally, the role of AMF in invasion ecology was examined, revealing their dual potential to either facilitate or hinder invasive plant species. Despite significant advances in understanding AMF biology, future research is needed to explore the underlying mechanisms of AMF-plant interactions and to address the challenges caused by changing environmental conditions. This review focused on the importance of AMF in promoting ecosystem resilience and suggests avenues for future research to harness their full potential in agricultural and ecological contexts.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Lactiplantibacillus plantarum LP5 for the bioremediation of wastewater effluent from a dairy product manufacturing plant. 植物乳杆菌LP5在某乳制品厂废水生物修复中的应用

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf071

María Julia Ruiz, Juan Pablo Conti, Daniel Fernández, Marcela Juliarena, Vanesa Fernández, Alejandro Bricker, Adriana Díaz, Nora Lía Padola, Silvina Etcheverría, Analía Inés Etcheverría

{"title":"Application of Lactiplantibacillus plantarum LP5 for the bioremediation of wastewater effluent from a dairy product manufacturing plant.","authors":"María Julia Ruiz, Juan Pablo Conti, Daniel Fernández, Marcela Juliarena, Vanesa Fernández, Alejandro Bricker, Adriana Díaz, Nora Lía Padola, Silvina Etcheverría, Analía Inés Etcheverría","doi":"10.1093/lambio/ovaf071","DOIUrl":"10.1093/lambio/ovaf071","url":null,"abstract":"The objective was to evaluate the capacity of Lactiplantibacillus plantarum LP5r to improve the microbiological and physicochemical quality of wastewater from a dairy company. Lactiplantibacillus plantarum LP5r was inoculated (8.8 log10 CFU g-1 mL-1) into 50 L containers of wastewater, 10 mL was added to T1, 10 mL to T2 with monthly replenishment, 50 mL to T3, and 50 mL to T4 with monthly replenishment. The CT was kept without inoculum. Initially, L. plantarum LP5r showed values above 6 log10 CFU g-1. In T1 and T3 decreased to undetectable values, but in T2 and T4 remained above 4 log10 CFU g-1. Enterobacteriaceae were initially 5.46 log10 CFU g-1 and decreased to 4.88 and 4.73 log10 CFU g-1 for T2 and T4 and increased to 7.31 log10 CFU g-1 in CT. Coliforms were reduced from 7.38 log10 CFU g-1 to 2.95 and 2.11 log10 CFU g-1 in T2 and T4. Initial suspended solids were 19 997 mg L-1, which decreased to 6058.3 mg L-1 and 4118.9 mg L-1 for CT and T4. The pH did not differ between samples. An initial BOD5 of 7680 mg L-1 was detected in all samples, which decreased to 2385 mg L-1 for CT and 820 mg L-1 for T4, and the COD decreased from 17 827 mg L-1 to 4839 mg L-1 and 1649 mg L-1 for the CT and T4 samples. Lactiplantibacillus plantarum LP5 positively influences the microbiological quality and COD and BOD5 of the dairy's wastewater.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing raw trout fillet quality with Lactiplantibacillus plantarum postbiotics and potassium sorbate. 植物乳杆菌后生制剂和山梨酸钾提高生鳟鱼鱼片品质。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf054

Amin Abbasi, Hadi Pourjafar, Mahdi Asghari Ozma, Mansoureh Taghizadeh, Hedayat Hosseini

{"title":"Enhancing raw trout fillet quality with Lactiplantibacillus plantarum postbiotics and potassium sorbate.","authors":"Amin Abbasi, Hadi Pourjafar, Mahdi Asghari Ozma, Mansoureh Taghizadeh, Hedayat Hosseini","doi":"10.1093/lambio/ovaf054","DOIUrl":"10.1093/lambio/ovaf054","url":null,"abstract":"This study investigates the impact of Lactiplantibacillus plantarum postbiotics (LPP) on microbiological and chemical alterations in raw rainbow trout fillets stored at 4°C. LPP, containing chemicals such as methyl ester, oleic acid, and eucalyptol, demonstrated considerable radical scavenging action and improved cellular safety, enhancing cell survival by 118.70% to 170.60% and elevating SOD (20.10-33.14 mU mg-1) and GSH-Px (99.80-120.30 mU mg-1) activity. The use of LPP (1.5%, 0.8%, 0.5%) and potassium sorbate (PS) in raw fish fillets resulted in a significant decrease in microbial proliferation, with the 1.5% LPP treatment achieving a reduction in Listeria monocytogenes count by 5.6 ± 0.07 log CFU g-1 by day 2. Additionally, chemical parameters showed marked stability; the pH value increased from 6.22 ± 0.08 to 6.4 ± 0.03 in the 1.5% LPP-treated fillets by day 12. Total volatile basic nitrogen levels for the same treatment remained below the acceptable threshold of 25 mg 100 g-1 until day 8 (21.23 ± 0.19 mg 100 g-1), while TBA levels peaked at 1.1 ± 0.02 mg kg-1 on day 12. This approach prolonged shelf life by up to 12 days, maintaining fillet quality with no adverse sensory consequences.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resuscitation-promoting factor diversity of Rhodococcus erythropolis KB1 and their promoting effect on bacterial growth and viable but non-culturable cell resuscitation. 红红红球菌KB1的复苏促进因子多样性及其对细菌生长和活细胞复苏的促进作用。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf068

Jianhui Fu, Jixiang Chen, Yonggang Wang, Dan Luo, Qingfang Zhang, Tianfeng Wang

{"title":"Resuscitation-promoting factor diversity of Rhodococcus erythropolis KB1 and their promoting effect on bacterial growth and viable but non-culturable cell resuscitation.","authors":"Jianhui Fu, Jixiang Chen, Yonggang Wang, Dan Luo, Qingfang Zhang, Tianfeng Wang","doi":"10.1093/lambio/ovaf068","DOIUrl":"https://doi.org/10.1093/lambio/ovaf068","url":null,"abstract":"Resuscitation-promoting factors (Rpfs) are proteins essential for reactivating microorganisms from the viable but non-culturable (VBNC) state and exhibit considerable diversity across bacterial species. Rhodococcus erythropolis KB1 is an efficient petroleum-degrading actinomycete with strong environmental adaptability. Its genome encodes a variety of Rpf proteins. In this study, we characterized these Rpfs through multiple sequence alignments, domain analysis, tertiary structure modeling, and biological activity assays. Each protein harbors a conserved Rpf domain of ∼70 amino acids and auxiliary domains such as DUF348, G5, and LysM. Reverse Transcription Quantitative Polymerase Chain (RT-qPCR) analysis revealed that rpf1 was highly expressed during the logarithmic phase in R. erythropolis KB1, while rpf4 and rpf5 exhibited lower expression levels. All recombinant proteins, expressed and purified from Escherichia coli, demonstrated muralytic activity. Notably, the muralytic activity was independent of auxiliary domain complexity, with Rpf5 showing the highest activity (18.24 ± 1.06 U nmol-1). Furthermore, all Rpfs promoted the growth and resuscitation of VBNC R. erythropolis KB1 cells, with Rpf5 providing the greatest effect. The efficiencies of the recombinant proteins in promoting bacterial growth and VBNC cell resuscitation varied significantly, and these variations were strongly correlated with their muralytic activities.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of multidrug resistant/extended spectrum beta-lactamase-producing Escherichia coli isolates belonging to high-risk clones from healthy 1-day-old broiler and layer chicks without in ovo or day-of-hatch antibiotic administration. 从健康的1日龄肉鸡和蛋鸡中发现多药耐药/产生广谱β -内酰胺酶的大肠杆菌分离株，属于高风险克隆，未给蛋或孵化日龄抗生素。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf062

Meki Boutaiba Benklaouz, Qada Benameur, Mohamed Salah Abbassi, Hebib Aggad

{"title":"Occurrence of multidrug resistant/extended spectrum beta-lactamase-producing Escherichia coli isolates belonging to high-risk clones from healthy 1-day-old broiler and layer chicks without in ovo or day-of-hatch antibiotic administration.","authors":"Meki Boutaiba Benklaouz, Qada Benameur, Mohamed Salah Abbassi, Hebib Aggad","doi":"10.1093/lambio/ovaf062","DOIUrl":"https://doi.org/10.1093/lambio/ovaf062","url":null,"abstract":"This study aimed to investigate the presence of antimicrobial resistant Escherichia coli (E. coli) among healthy day-old broiler and layer chicks in the absence of in ovo or day-of-hatch antibiotic administration. A total of 100 pooled samples from 14 hatcheries across western Algeria were collected for analyses. Antimicrobial susceptibility testing was performed using the disc-diffusion method. Genes encoding antibiotic resistance, integrons, and phylogenetic groups were screened using Polymerase Chain Reaction (PCR), with the genetic relatedness of extended spectrum beta-lactamase (ESBL)-producing isolates determined via multilocus sequence typing. Sixty-eight samples contained E. coli, with high levels of resistance to multiple antibiotics found among broiler (92.10%) and layer chicks (100%) (no significant statistical association, P < 0.05). Multidrug-resistant (MDR)/ESBL-producing isolates were detected in samples from both broiler (n = 11) and layer (n = 2) hatcheries, from which three and five harbored blaCTX-M-1 and blaCTX-M-14, respectively. Additionally, tetA (n = 7), sul1 (n = 5), aac(6´)-Ib-cr (n = 2), and int1 (n = 7) genes were detected. Isolates belonged to the clones ST10 (n = 1), ST617 (n = 1), ST405 (n = 3), ST69 (n = 4), ST224 (n = 3), and ST4494 (n = 1). Study findings indicate that even in the absence of any prior antibiotic administration, day-old chicks in western Algerian hatcheries carry MDR isolates capable of spreading across the national poultry sector, representing a significant public health concern.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenobiotics mediated modulation of gut microbiota and its role in lifestyle diseases: a critical appraisal on exposomics. 外源药物介导的肠道菌群调节及其在生活方式疾病中的作用：对暴露组学的重要评价。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf067

Arush Behl, Krishna Kant Sharma

{"title":"Xenobiotics mediated modulation of gut microbiota and its role in lifestyle diseases: a critical appraisal on exposomics.","authors":"Arush Behl, Krishna Kant Sharma","doi":"10.1093/lambio/ovaf067","DOIUrl":"https://doi.org/10.1093/lambio/ovaf067","url":null,"abstract":"Gastrointestinal tract of humans provides a niche to thousands of microbes, referred as gut microbiota (GM). GM establishes an intricate relationship with other organs via gut-organ axis, and modulates host health. The structure and functioning of these gut microbes can be influenced by the type of external exposome an individual experiences. Depending upon GM perturbations and host genotype, this can result in variable health implications. On the other hand, the huge arsenal of enzymes possessed by GM can chemically alter the xenobiotic structure. Its consequences can be numerous, including formation of harmful metabolites that cause organ damage, reversal of host detoxification pathways, or favourable health effects. Additionally, GM-mediated bio-transformation of pharmaceuticals can alter their pharmacokinetics and pharmacodynamics, potentially yielding variable drug responses, resulting into prolonged or ineffective treatments. To address this bi-facial relationship and the pivotal role of GM, this review incorporates recent in vitro, in vivo, and multiomics studies. It also suggests the need of machine learning approaches to decode the complex host-microbiota-xenobiotics interactions. These knowledge will aid in comprehending recent rise in chronic lifestyle-diseases which poses a huge burden on the health sector, and can also be a learning curve in making formulations and therapies for personalized treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic and molecular insights into adenosine triphosphate synthase inhibitors from Micromonospora sp. as novel antimicrobial agents against multidrug-resistant Gram-negative pathogens of enteric origin. 代谢组学和分子研究表明，来自小单孢菌的三磷酸腺苷合成酶抑制剂可作为抗肠源多重耐药革兰氏阴性病原体的新型抗菌剂。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf064

Sahar A El-Shatoury, Hanan K Megawer, Amro M Hanora, Rabab R Makharita, Raúl Riesco, Martha E Trujillo, Mohamed S Nafie

{"title":"Metabolomic and molecular insights into adenosine triphosphate synthase inhibitors from Micromonospora sp. as novel antimicrobial agents against multidrug-resistant Gram-negative pathogens of enteric origin.","authors":"Sahar A El-Shatoury, Hanan K Megawer, Amro M Hanora, Rabab R Makharita, Raúl Riesco, Martha E Trujillo, Mohamed S Nafie","doi":"10.1093/lambio/ovaf064","DOIUrl":"https://doi.org/10.1093/lambio/ovaf064","url":null,"abstract":"The overuse of antibiotics has accelerated the emergence of antibiotic-resistant bacteria, necessitating alternative treatment options. Micromonospora spp., known for producing bioactive metabolites, is a promising source of novel antimicrobials. This study evaluated the antimicrobial potential of metabolic ethyl-acetate extracts from three Micromonospora strains against multidrug-resistant (MDR) Gram-negative clinical isolates of enteric origin. Using the Kirby-Bauer modified disc diffusion method, following Clinical and Laboratory Standards Institute guidelines, the extract from Micromonospora strain 65SH exhibited the most potent activity, with minimum inhibitory concentrations of 25 µg/ml against Enterobacter aerogenes and 12.5 µg/ml against Escherichia coli. 16S rRNA gene sequencing identified the strain as closely related to Micromonospora fluminis (99.6% similarity). Further analysis using LC-QTOF-MS/MS non-targeted metabolomics identified six bioactive compounds-melibiose, oligomycin A, queuine, heptelidic acid, diethyl phthalate, and 2'-deoxyguanosine-linked to the inhibition of bacterial enzymes essential for proliferation. Molecular modeling suggested that these compounds disrupt E. coli ATP synthase and inhibit ATP-dependent bacterial topoisomerases. This study integrates metabolomics, molecular docking, and genomics, offering robust mechanistic insights into ATP synthase inhibition. Future research will include fecal isolate testing, detailed structural elucidation using nuclear magnetic resonance (NMR) spectroscopy, and experimental validation to explore the therapeutic potential of Micromonospora-derived compounds.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular mycorrhizal fungal symbiosis in New Zealand ecosystems: challenges and opportunities. 新西兰生态系统中的丛枝菌根真菌共生：挑战和机遇。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 Epub Date: 2025-05-09 DOI: 10.1093/lambio/ovaf070

Romy Moukarzel, Lauren P Waller, E Eirian Jones, Hayley J Ridgway

{"title":"Arbuscular mycorrhizal fungal symbiosis in New Zealand ecosystems: challenges and opportunities.","authors":"Romy Moukarzel, Lauren P Waller, E Eirian Jones, Hayley J Ridgway","doi":"10.1093/lambio/ovaf070","DOIUrl":"10.1093/lambio/ovaf070","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs that form a symbiotic and mutualistic relationship with most terrestrial plants, playing an important role in plant growth, nutrient acquisition, and ecosystem stability. This review synthesizes current knowledge on AMF colonization in plants within New Zealand ecosystems, including the challenges and opportunities of molecular identification techniques used in characterizing AMF communities in natural and managed systems. The ecosystem services provided by AMF, such as improved growth parameters, enhanced nutrition, and disease control, are discussed in detail, highlighting their significance in sustainable agriculture and natural ecosystems. Additionally, the role of AMF in invasion ecology was examined, revealing their dual potential to either facilitate or hinder invasive plant species. Despite significant advances in understanding AMF biology, future research is needed to explore the underlying mechanisms of AMF-plant interactions and to address the challenges caused by changing environmental conditions. This review focused on the importance of AMF in promoting ecosystem resilience and suggests avenues for future research to harness their full potential in agricultural and ecological contexts.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life history strategies to study the succession of H2-oxidizing bacteria and their ecological role along H2 concentration gradients in upland soils. 陆地土壤H2氧化细菌演替的生活史策略及其沿H2浓度梯度的生态作用

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf061

Xavier Baril, Philippe Constant

{"title":"Life history strategies to study the succession of H2-oxidizing bacteria and their ecological role along H2 concentration gradients in upland soils.","authors":"Xavier Baril, Philippe Constant","doi":"10.1093/lambio/ovaf061","DOIUrl":"https://doi.org/10.1093/lambio/ovaf061","url":null,"abstract":"The recent discovery of atmospheric chemosynthesis has caused a paradigm shift in the way H2-oxidizing bacteria (HOB) are examined. The field has transitioned from the consideration of HOB as a specialized functional group benefiting from the energy potential of H2 for mixotrophic growth or persistence to a versatile group of bacteria using multiple trace gases. We discuss four life history strategies supported by H2, namely chemolithoautotrophic growth, mixotrophic growth, persistence, and atmospheric chemosynthesis. There is experimental evidence supporting the role of HOB in various ecosystem services beyond the uptake of H2 including, for instance, carbon cycling, plant growth promotion, and primary production. Decoupling between the intensity of HOB activation in soil and compositional change of microbial communities remains puzzling, highlighting our poor understanding of the ecological role of HOB. We call for new experimental approaches to delineate the interactions between HOB and the other members of the community. We propose a dedicated framework integrating life history strategies of HOB for mechanistic assessment of microbial interactions and processes supported by H2 in soil.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a high-throughput xMAP technology for detection of 16 pathogens in raw milk. 建立高通量xMAP技术检测原料奶中16种病原菌。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-05-01 DOI: 10.1093/lambio/ovaf063

Na Liu, Sha Xiao, Jianmei Zhao, Yubin Gao, Xiumei Huang, Xiyue Zhang, Lin Wang, Ge Zhao, Junwei Wang

{"title":"Establishment of a high-throughput xMAP technology for detection of 16 pathogens in raw milk.","authors":"Na Liu, Sha Xiao, Jianmei Zhao, Yubin Gao, Xiumei Huang, Xiyue Zhang, Lin Wang, Ge Zhao, Junwei Wang","doi":"10.1093/lambio/ovaf063","DOIUrl":"https://doi.org/10.1093/lambio/ovaf063","url":null,"abstract":"To establish a rapid detection method for pathogens potentially contaminating raw milk, species-specific primers and probes were designed and modified for the simultaneous identification of 16 different target pathogens. Probes were coupled with fluorescent carboxylated microspheres, while specific positive plasmids and strains constructed previously were positive controls. Critical reaction parameters, including hybridization temperature (52°C) and duration (20 min), were systematically optimized. The assay demonstrated excellent specificity, with each probe exhibiting distinct fluorescence signals and no observable cross-hybridization. Analytical sensitivity test revealed detection limits of 10 CFU mL-1 for eight pathogens, and 102 CFU mL-1 for the remaining eight. Reproducibility was confirmed, with coefficients of variation ranging from 6.23% to 13.4%. Validation using artificially contaminated raw milk samples (six pathogens) demonstrated similar results between the xMAP assay and two reference methods (PCR and culture-based). The established xMAP-based multiplex detection system exhibits high specificity, sensitivity, and throughput, demonstrating its applicability for routine monitoring of raw milk pathogens.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":"78 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0