Letters in Applied Microbiology最新文献_第2页

A multilocus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme. 新兴呼吸道病原体双形支原体的多位点序列分型方案。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf073

Lily Simpson, Joshua Macleod, Richard S Rowlands, Owen B Spiller, Charlotte Dixon, Jorgen S Jensen, Baharak Afshar, Helena M B Seth-Smith, Victoria J Chalker, Michael L Beeton

{"title":"A multilocus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme.","authors":"Lily Simpson, Joshua Macleod, Richard S Rowlands, Owen B Spiller, Charlotte Dixon, Jorgen S Jensen, Baharak Afshar, Helena M B Seth-Smith, Victoria J Chalker, Michael L Beeton","doi":"10.1093/lambio/ovaf073","DOIUrl":"10.1093/lambio/ovaf073","url":null,"abstract":"Mycoplasma amphoriforme is an emerging respiratory pathogen for which little is known about the population structure or transmission dynamics. In this study, we developed the first multilocus sequence typing (MLST) scheme for M. amphoriforme and applied it to a previous genomic data set. The genomes of seven M. amphoriforme isolates from the UK and Denmark were sequenced and used to develop the MLST scheme based on loci used for previous Mycoplasma MLST schemes. The resulting MLST scheme consisted of four loci (gyrB, atpG, uvrA, and rpoB) and was applied to 20 previously sequenced genomes obtained from the UK and France/Tunisia. From the 27 sequences examined, 13 sequence types were identified. A phylogenetic tree of concatenated sequences showed a comparable topology to a previously described tree based on whole genome data. Additionally, the MLST scheme corroborated the previous suggestion of possible healthcare-associated transmission of M. amphoriforme between two separate patients. The MLST scheme gave a population structure analysis comparable to previous whole-genome-based analyses.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atoxigenic isolates of Aspergillus flavus effectively reduce cyclopiazonic acid in a sorghum-based matrix under simulated abiotic stress conditions. 模拟非生物胁迫条件下，黄曲霉产氧分离株在高粱基质中有效还原环吡唑酸

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf072

Vanshika Sharma, Carla Cervini, Carol Verheecke-Vaessen, Ranajit Bandyopadhyay, Angel Medina, Alejandro Ortega-Beltran, Naresh Magan

{"title":"Atoxigenic isolates of Aspergillus flavus effectively reduce cyclopiazonic acid in a sorghum-based matrix under simulated abiotic stress conditions.","authors":"Vanshika Sharma, Carla Cervini, Carol Verheecke-Vaessen, Ranajit Bandyopadhyay, Angel Medina, Alejandro Ortega-Beltran, Naresh Magan","doi":"10.1093/lambio/ovaf072","DOIUrl":"10.1093/lambio/ovaf072","url":null,"abstract":"Maize, groundnut, and sorghum are important staple crops in several countries, but are prone to mycotoxin contamination. In the tropics and subtropics, Aspergillus flavus frequently contaminates those crops with aflatoxins and, sometimes, with cyclopiazonic acid (CPA). However, some genotypes cannot produce one or both toxins. In various countries, atoxigenic isolates of A. flavus are formulated into biocontrol products for field use to outcompete aflatoxin producers. The products effectively limit aflatoxin but their utility to reduce CPA remains unexplored. The abilities of four atoxigenic isolates (AF-) from Burkina Faso to control CPA by an isolate with high capacity to produce aflatoxins (AF+) and CPA was tested in co-inoculations at varying ratios (100+, 75+/25-, 50+/50-, 25+/75-, 100-), under simulated abiotic stress conditions. Experiments were conducted on 2% sorghum-based media at 0.95 and 0.90 water activity (aw), at 30°C and 37°C, for 12 days. CPA was quantified using liquid chromatography tandem mass spectroscopy. CPA concentrations gradually decreased as the proportion of atoxigenic isolates increased, with effectiveness varying depending on the environmental conditions.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Typical skin and oral bacterial species present in human milk are not the result of contamination during the sampling process. 母乳中存在的典型皮肤和口腔细菌种类不是采样过程中污染的结果。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf084

Lisa F Stinson, Donna T Geddes

{"title":"Typical skin and oral bacterial species present in human milk are not the result of contamination during the sampling process.","authors":"Lisa F Stinson, Donna T Geddes","doi":"10.1093/lambio/ovaf084","DOIUrl":"10.1093/lambio/ovaf084","url":null,"abstract":"Human milk has a low microbial biomass with a microbiome dominated by typical skin and oral taxa, raising concerns about contamination during sample collection. However, to date, no study has directly compared samples collected with and without aseptic technique, leaving questions related to potential contamination within the field. To address this, we compared the microbiota of hand-expressed milk samples collected from 23 mothers before and after cleansing of the hands and breast. Metataxonomic analysis showed that taxonomic profiles were largely unaffected by cleansing, with only Rothia mucilaginosa significantly more abundant in non-aseptically collected samples (P = 0.007). Although aseptically and non-aseptically collected samples were taxonomically similar, there was a higher level of bacterial richness (P = 0.003) and evenness (Shannon diversity, P = 0.0002) in non-aseptically collected samples, suggesting that multiple low-abundance taxa are introduced via skin contamination. These findings support the use of aseptic collection methods to minimize external contamination and accurately assess milk microbial diversity. Importantly, they also suggest that common skin and oral taxa detected in human milk are likely true members of the mammary microbiome.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of phosphorus on genome copy number of Synechocystis sp. PCC 6803. 磷对聚囊藻pcc6803基因组拷贝数的影响。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf087

Junkui Zhao, Mengting Wang, Chengwei Liang, Yongzhong Lu

{"title":"Influence of phosphorus on genome copy number of Synechocystis sp. PCC 6803.","authors":"Junkui Zhao, Mengting Wang, Chengwei Liang, Yongzhong Lu","doi":"10.1093/lambio/ovaf087","DOIUrl":"10.1093/lambio/ovaf087","url":null,"abstract":"The regulatory mechanisms underlying ploidy dynamics in cyanobacteria under phosphorus (P) limitation remain poorly understood. In this study, we investigated the impact of phosphate deprivation on the polyploidy of Synechocystis sp. PCC 6803 through integrated approaches combining spectrofluorometry, flow cytometry, comparative transcriptomics, Pho regulon prediction, and enzymatic activity assays. Our results revealed a significant reduction in genomic DNA content (P < 0.01) during cultivation in phosphate-free BG11 medium, with average genome copy numbers decreasing from 24.34 ± 0.27 in standard BG11 to 6.18 ± 0.25 under P-depleted conditions (P < 0.01). Transcriptomic analysis demonstrated marked upregulation of genes associated with two-component signaling systems, ABC transporters, and nucleotide metabolism, while DNA replication, homologous recombination, and mismatch repair pathways were significantly downregulated (P < 0.05). Concurrently, alkaline phosphatase activity exhibited a substantial increase (P < 0.01), suggesting enhanced phosphate mobilization. These findings collectively indicated that genome copy number in Synechocystis sp. PCC 6803 was dynamically regulated through the coordinated interplay between DNA replication suppression and degradation activation in response to phosphorus availability. This work provides novel insights into the molecular basis of ploidy regulation in cyanobacteria and offers valuable implications for understanding analogous mechanisms in chloroplasts.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving maize growth through biopriming with seed endophytic Bacillus tequilensis LRB17. 龙舌兰内生芽孢杆菌LRB17生物雾化促进玉米生长

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf074

Soumya Sephalika Swain, Madhusmita Ghana, Shubhransu Nayak

{"title":"Improving maize growth through biopriming with seed endophytic Bacillus tequilensis LRB17.","authors":"Soumya Sephalika Swain, Madhusmita Ghana, Shubhransu Nayak","doi":"10.1093/lambio/ovaf074","DOIUrl":"10.1093/lambio/ovaf074","url":null,"abstract":"Maize (Zea mays L.) is the third most cultivated crop globally after wheat and rice. It is one of the important staple food crops in the tribal-inhabited forest areas in India, where reduced yield is observed due to lower soil nutrients. Biopriming with bacteria having plant growth promotion (PGP) effects could be an effective way to mitigate this problem. In this context, a potential Bacillus tequilensis LRB17 strain was bioprimed in maize cultivar for the first time for PGP. Treatment of maize with the bacterium could enhance seedling vigor by 19.56%. The root and shoot length and the root and shoot weights were enhanced by 120.21% and 121.41% and 43.03% and 40.07%, respectively. Average height of matured plants, average leaf area, and plant biomass could be enhanced by 19.30%, 30.47%, and 311.30%, respectively. Parenchyma cells in stems increased with well-developed bundle sheaths. The bacterium has been isolated from local millet crop landrace of \"Koraput\" area of the \"Eastern Ghats\" of India, which has been designated as \"Globally Important Agricultural Heritage System (GIAHS)\" by the United Nations. Hence, the native Bacillus tequilensis LRB17 posed as a potential bioagent for yield enhancement of maize for the benefit of tribal population.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A two-group quadruplex real-time quantitative PCR assay for molecular patho-serotyping of Yersinia enterocolitica. 结肠炎耶尔森菌分子病理分型的两组四重实时定量PCR方法。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf079

Jing Wang, Jingliang Qin, Bin Hu, Yijia Wang, Boyang Cao, Xi Guo

{"title":"A two-group quadruplex real-time quantitative PCR assay for molecular patho-serotyping of Yersinia enterocolitica.","authors":"Jing Wang, Jingliang Qin, Bin Hu, Yijia Wang, Boyang Cao, Xi Guo","doi":"10.1093/lambio/ovaf079","DOIUrl":"10.1093/lambio/ovaf079","url":null,"abstract":"Yersinia enterocolitica is one of the most important foodborne pathogens with significant impact on public health. It can be divided into six biotypes and approximately 60 O-serotypes, with O:3, O:9, O:8, and O:5,27 being predominantly associated with human yersiniosis. We present a two-group quadruplex real-time quantitative PCR (RT‒qPCR) for the patho-serotyping of Y. enterocolitica. The design of primers and probes within group 1 was based on sero-specific genes within the O antigen gene cluster, and those within group 2 were selected from the virulence markers and the restriction modification system to distinguish O:5,27 from O:5. The specificity was tested using reference strains, and was confirmed by a comparison with those obtained by a previous multiplex PCR. The limit of detection is 0.1 ng, or 104 copies μl-1 of genomic DNA, and the standard curves exhibited high linearity and correlation coefficients, demonstrating our assay's robustness. Among the 81 isolates used to evaluate the reproducibility, the results for 76 were consistent between the two approaches, indicating that the sensitivity of our RT‒qPCR is 100%, and the positive predictive value is 94%. Our assay can serve as a tool for identifying sources of Y. enterocolitica contamination and for epidemiological monitoring of this bacterium.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila upregulates the expression of γ-aminobutyric acid in response to deoxycholate exposure. 嗜muciniphila在脱氧胆酸暴露下上调γ-氨基丁酸的表达。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf077

Tatsuro Hagi, Masaru Nomura, Clara Belzer

{"title":"Akkermansia muciniphila upregulates the expression of γ-aminobutyric acid in response to deoxycholate exposure.","authors":"Tatsuro Hagi, Masaru Nomura, Clara Belzer","doi":"10.1093/lambio/ovaf077","DOIUrl":"10.1093/lambio/ovaf077","url":null,"abstract":"Akkermansia muciniphila is a beneficial gut bacterium because of its improving metabolic effect. However, it is not fully understood how A. muciniphila interacts with host substances to inhabit the human gut. To examine the effect of deoxycholate (DCA) produced by the combination of host and gut bacteria, which enhances the growth of A. muciniphila, on the metabolic changes of A. muciniphila using transcriptome and proteome analyses. Transcriptome analysis showed that carbohydrate metabolism, including glycosyl hydrolase activity and glycosyl bond activity, was significantly upregulated. Notably, transcriptome and proteome analyses demonstrated that the γ-aminobutyric acid (GABA) production pathway, which is related to acid or osmotic stress responses, was upregulated in the presence of DCA. Our results demonstrated that carbohydrate metabolism and GABA production were altered in response to DCA. Therefore, DCA may be a key intestinal substance for the physiological regulation and persistence of A. muciniphila in the gut. This study provides valuable insights into understanding the interaction between host and gut bacterium to persist in the gut.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional paddy cultivated soil-borne bacteria: potential biofertilizer. 多功能水稻栽培土传细菌：潜在的生物肥料。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf082

Gamze Kurtuluş, Emel Kayali, Nüzhet Cenk Sesal, Filiz Vardar

{"title":"Multifunctional paddy cultivated soil-borne bacteria: potential biofertilizer.","authors":"Gamze Kurtuluş, Emel Kayali, Nüzhet Cenk Sesal, Filiz Vardar","doi":"10.1093/lambio/ovaf082","DOIUrl":"10.1093/lambio/ovaf082","url":null,"abstract":"Biofertilizers have gained attention as eco-friendly alternatives to mitigate the adverse effects of chemical fertilizers. This study focuses on recycling paddy straw waste using lignocellulolytic soil-borne bacteria isolated from paddy fields in Kırklareli and Samsun, Türkiye. This approach supports soil organic matter enrichment and reduces chemical fertilizer dependence, thus lowering greenhouse gas emissions. Microorganisms were isolated using selective media. Cellulolytic and ligninolytic activities were assessed via DNS and Azure-B methods, respectively. Among the isolates, Bacillus sp. S2 (Samsun) exhibited the highest enzymatic activities and grew on nitrogen-free Jensen's medium, suggesting potential nitrogen-fixing ability. Pseudomonas sp. K2 (Kırklareli) showed moderate but consistent lignocellulolytic activity. Following 30 days of incubation with powdered paddy straw, gas chromatography-mass spectrometry (GC-MS) analysis revealed that S2 produced the highest levels of 4-coumaric acid (2.371%), benzoic acid (3.019%), and formic acid (1.280%), along with xylitol (1.390%) and 1-triacontanol (1.298%)-compounds with agronomic relevance for plant growth, stress tolerance, and soil health. K2 uniquely produced arabitol, associated with osmoprotection. BLAST analysis showed that S2 shares 98% identity with Bacillus velezensis and B. amyloliquefaciens, while K2 showed 95% similarity to Ectopseudomonas chengduensis, E. alcaliphila, and P. sihuiensis. These traits support their potential use in sustainable agriculture and crop residue management.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutagenesis and transcriptomic analysis of a highly pathogenic Alternaria alternata DT-DYLC against Chenopodium album L. 高致病性交替稻瘟病菌DT-DYLC对Chenopodium album L的诱变及转录组学分析

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf080

Yuzhe Wang, Haiyang Cheng, Hongyu Cheng, Juan Li, Xin Wang, Haixia Zhu, Youhai Wei, Liang Cheng

引用次数: 0

Enhanced recombinase polymerase amplification via UvsX engineering and reaction optimization for rapid detection of Vibrio parahaemolyticus. 通过UvsX工程增强重组酶聚合酶扩增和反应优化快速检测副溶血性弧菌。

IF 2 4区生物学

Letters in Applied Microbiology Pub Date : 2025-06-02 DOI: 10.1093/lambio/ovaf081

Lin Wang, Yiming Li, Pengbo Wang, Yibei Zhang, Qin Liu

{"title":"Enhanced recombinase polymerase amplification via UvsX engineering and reaction optimization for rapid detection of Vibrio parahaemolyticus.","authors":"Lin Wang, Yiming Li, Pengbo Wang, Yibei Zhang, Qin Liu","doi":"10.1093/lambio/ovaf081","DOIUrl":"10.1093/lambio/ovaf081","url":null,"abstract":"Recombinase polymerase amplification (RPA) is a powerful isothermal nucleic acid amplification technique, yet its efficiency is critically dependent on the catalytic efficiency of the recombinase UvsX, a key enzyme mediating homologous DNA pairing and strand exchange. To address this limitation, in this study, we developed a specific, sensitive, and robust RPA detection method by optimizing the UvsX enzyme through protein engineering and refining the RPA reaction system. By conducting comparative structural and functional analysis of UvsX orthologs from 13 Myoviridae phages, we identified critical determinants of recombinase activity within the Loop 2 domain of T4 UvsX. Furthermore, we systematically optimized the stoichiometric ratios of core enzymes and crowding agents to establish a robust RPA system. This system was subsequently integrated with lateral flow strips for point-of-need detection of highly lethal Vibrio parahaemolyticus in shrimp. Our results demonstrated that the engineered UvsXv1 variant exhibited significantly improved strand displacement activity, leading to enhanced RPA amplification efficiency and stability.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0