Letters in Applied Microbiology最新文献_第2页

Immunomodulatory effects of Lactiplantibacillus paraplantarum BAL-28-ITTG paraprobiotics and postbiotics. 副植物乳杆菌BAL-28-ITTG副益生菌和后益生菌的免疫调节作用。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag033

Aurora Anahí Ocampo-Zaragoza, Alicia Grajales-Lagunes, Sandy Luz Ovando-Chacón, María Celina Luján-Hidalgo, Victor Manuel Ruíz-Valdiviezo, Miguel Angel Ruiz-Cabrera, Luz Eugenia Alcántara-Quintana, Miguel Abud-Archila

{"title":"Immunomodulatory effects of Lactiplantibacillus paraplantarum BAL-28-ITTG paraprobiotics and postbiotics.","authors":"Aurora Anahí Ocampo-Zaragoza, Alicia Grajales-Lagunes, Sandy Luz Ovando-Chacón, María Celina Luján-Hidalgo, Victor Manuel Ruíz-Valdiviezo, Miguel Angel Ruiz-Cabrera, Luz Eugenia Alcántara-Quintana, Miguel Abud-Archila","doi":"10.1093/lambio/ovag033","DOIUrl":"10.1093/lambio/ovag033","url":null,"abstract":"Intracellular paraprobiotics (PP) and postbiotics (PB) derived from lactic acid bacteria are being increasingly explored as alternatives to conventional probiotics. We examined the immunomodulatory effects of PP and PB obtained from Lactiplantibacillus paraplantarum BAL-28-ITTG using human intestinal epithelial cell (HIEC-6) and dendritic cell (NHDC) lines. PP and PB were obtained by thermal and thermal-sonication treatments and characterized by scanning electron microscopy, fatty acid profiling (Gas Chromatography - Mass Spectrometry (GC-MS)), and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS‒PAGE). Cellular responses were assessed through metabolic activity (MTT), catalase/caspase activity, and multiplex cytokine quantification. Statistical analyses were performed using Analysis of Variance (ANOVA) and appropriate post hoc tests. PP and PB did not induce cytotoxic effects in HIEC-6 or NHDC cells. PP treatment increased cellular metabolic activity in a dose-dependent manner and modulated the secretion of cytokines, particularly IL-1β, IL-4, IL-6, IL-10, and TNF-α. Interestingly, PP-2 enhanced cytokine production under basal conditions while attenuating cytokine responses in lipopolysaccharide-stimulated cultures. These findings demonstrate that BAL-28-ITTG-derived PP and PB modulate cytokine responses in human intestinal and immune cell models in vitro, warranting further investigation to determine their biological relevance in vivo.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of allyl isothiocyanate vapor on Salmonella enterica inactivation and quality characteristics of blueberries. 异硫氰酸烯丙酯蒸气对蓝莓肠沙门氏菌灭活及品质特性的影响。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag032

Eungyeong Kim, Huyong Lee, In Hee Cho, Jee-Hoon Ryu, Hoikyung Kim

{"title":"Effects of allyl isothiocyanate vapor on Salmonella enterica inactivation and quality characteristics of blueberries.","authors":"Eungyeong Kim, Huyong Lee, In Hee Cho, Jee-Hoon Ryu, Hoikyung Kim","doi":"10.1093/lambio/ovag032","DOIUrl":"10.1093/lambio/ovag032","url":null,"abstract":"This study examined the antimicrobial efficacy of allyl isothiocyanate (AITC) vapor against three Salmonella enterica serotypes (Salmonella Agona, Salmonella Cubana, and Salmonella Newport) on blueberries and assessed their effects on fruit quality. AITC vapors were generated from 300, 600, and 900 µl ml-1 solutions and applied at 12°C or 25°C. At 25°C, Population of S. Agona and S. Cubana were reduced to below the detection limit within 10 and 8 h, respectively, at all concentrations, while S. Newport required exposure of 900 µl ml-1 vapor for over 6 h to achieve a similar reduction. Antimicrobial activity increased with temperature, concentration, and exposure time. Quality analysis showed no significant change in L* and a* values, but b* values decreased after treatment at 25°C for more than 10 h, indicating enhanced blue intensity. Hardness of fruit decreased faster at 25°C than at 12°C, suggesting temperature-dependent softening. Sensory evaluation revealed no major differences in color or odor except for odor at the highest concentration, but texture changed significantly. These findings suggest that AITC vapor can effectively control Salmonella on blueberries while treatment conditions require optimization to maintain fruit quality.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ciprofloxacin-functionalized silver nanocomposite: a promising strategy against colistin-resistant Pseudomonas aeruginosa. 环丙沙星功能化银纳米复合材料：抗耐粘菌素铜绿假单胞菌的有希望的策略。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag027

Sameera Mujahid, Samiya Farooq, Absar Talat, Jawed Iqbal, Kafil Akhtar, Asad U Khan

{"title":"Ciprofloxacin-functionalized silver nanocomposite: a promising strategy against colistin-resistant Pseudomonas aeruginosa.","authors":"Sameera Mujahid, Samiya Farooq, Absar Talat, Jawed Iqbal, Kafil Akhtar, Asad U Khan","doi":"10.1093/lambio/ovag027","DOIUrl":"10.1093/lambio/ovag027","url":null,"abstract":"The rapid rise of colistin resistance in Pseudomonas aeruginosa presents a critical challenge, necessitating innovative therapeutic alternatives. In this study, we developed a novel ciprofloxacin-functionalized silver nanocomposite (NC) by modifying phospholipid-PEG (polyethylene glycol)-coated silver nanoparticles to enhance antibacterial efficacy against colistin-resistant P. aeruginosa. The NC was comprehensively characterized by UV-Visible spectroscopy, dynamic light scattering, Fourier transform infrared spectroscopy, transmission electron microscope, scanning electron microscopy, and EDX, confirming spherical morphology with an average size of 29.74 nm. Biological evaluations revealed potent antibacterial and antibiofilm activity: CFU (colony forming unit) and crystal violet assays demonstrated a 6.9 log₁₀ CFU mL-1 reduction in bacterial colonies and a 31.45% decrease in biofilm formation. Mechanistic studies revealed an elevated production of reactive oxygen species, singlet oxygen, and hydroxyl radicals upon treatment, correlating with a 39.70% decline in bacterial viability in XTT assays. Importantly, methyl thiazolyl tetrazolium cytotoxicity tests against MLE12 cells confirmed the NC's biocompatibility. Efficacy was further validated in an in vivo lung infection model in albino Wistar rats, where histopathological analysis and cytokine profiling confirmed therapeutic potential. Collectively, these findings establish ciprofloxacin-modified silver NCs as a stable, safe, and highly effective strategy for treating multidrug-resistant P. aeruginosa, offering a promising alternative to colistin in combating life-threatening lung infections.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147645754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Naproxen and ampicillin degradation by the Ascomycota fungi Penicillium and Aspergillus. 子囊菌属真菌青霉菌和曲霉降解萘普生和氨苄西林的研究。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag040

Mailyn S Porras-Garcia, Alma D Paz-González, Ana V Martínez-Vázquez, Timoteo Delgado-Maldonado, Eyra Ortiz-Pérez, Gildardo Rivera

{"title":"Naproxen and ampicillin degradation by the Ascomycota fungi Penicillium and Aspergillus.","authors":"Mailyn S Porras-Garcia, Alma D Paz-González, Ana V Martínez-Vázquez, Timoteo Delgado-Maldonado, Eyra Ortiz-Pérez, Gildardo Rivera","doi":"10.1093/lambio/ovag040","DOIUrl":"10.1093/lambio/ovag040","url":null,"abstract":"The global population has increased the use of pharmaceutical compounds, emerging as contaminants. Due to their persistence in the environment, pharmaceutical residues have been detected in water and soil sources, causing adverse effects on ecosystems. Therefore, methods and techniques are necessary to degrade this pollution. An environmentally sustainable option is fungal remediation, which can decompose or degrade various xenobiotics. In this study, 30 phenanthrene-tolerant fungal isolates were analyzed to determine their potential for pharmaceutical degradation. Nine isolates were tolerant to 100 mg L-1 and three to 200 mg L-1 of naproxen and ampicillin. An enzyme activity analysis detected the production of laccase and manganese peroxidase. Penicillium crustosum H10 causes a high degradation of naproxen 95 % at 5 mg L-1 in 7 days and 100 % at 50 mg L-1 in 15 days and a low degradation of ampicillin (11.15% at 5 mg L-1). These results encourage exploring P. crustosum H10 as a viable alternative for the elimination of non-steroidal anti-inflammatory drugs.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic lipopeptides from Bacillus velezensis differentially affect spore germination and cell membranes in fungal plant pathogens. 来自velezensis芽孢杆菌的环状脂肽对真菌植物病原体孢子萌发和细胞膜的影响存在差异。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag035

Hannah Schott, Irina Popescu, Caitlin Kehoe, Marie Ouangni, Erika Wareberg, Tyler J Avis

{"title":"Cyclic lipopeptides from Bacillus velezensis differentially affect spore germination and cell membranes in fungal plant pathogens.","authors":"Hannah Schott, Irina Popescu, Caitlin Kehoe, Marie Ouangni, Erika Wareberg, Tyler J Avis","doi":"10.1093/lambio/ovag035","DOIUrl":"10.1093/lambio/ovag035","url":null,"abstract":"This study investigated the antifungal and mechanistic activity of two Bacillus velezensis strains against the mycelial and spore morphologies of the plant pathogens Alternaria solani (tomato/potato early blight), Cladosporium cucumerinum (cucurbit scab), and Fusarium sambucinum (potato dry rot). The pathogens were tested against B. velezensis strains SHRb and SHRc and their secondary metabolites to evaluate their ability to suppress fungal growth. The effects of Bacillus cyclic lipopeptides on spore germination and membrane permeability were assessed and compared to the ability of B. velezensis SHRb and SHRc precipitates to alter spore membrane permeability. Confrontation assays and treatments with filtrates demonstrated that both strains inhibited mycelial growth of all three fungi by up to 52% and 79%, respectively. Conversely, the cyclic lipopeptides exhibited varying abilities to inhibit spore germination and increase membrane permeability (up to 7.8-fold over the control). The lipopeptide iturin was consistently the most inhibitory of spore germination for all fungi. Membrane permeability assays indicated that the mode of action of B. velezensis SHRb likely includes membrane disruption, while B. velezensis SHRc may act through alternative antimicrobial mechanisms. Spore germination was differentially sensitive when compared to mycelial growth, implying that specific lipopeptide effects may be distinct depending on morphological state.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repurposing onion peel waste as a cost-effective and sustainable substrate for xanthan production. 利用洋葱皮废料作为成本效益和可持续的黄原胶生产基质。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-06 DOI: 10.1093/lambio/ovag038

Kriti Dutta, Rahul Dey, Rituparna Debnath, Sabrina Sultana Rahman, Biswa Prasun Chatterji

{"title":"Repurposing onion peel waste as a cost-effective and sustainable substrate for xanthan production.","authors":"Kriti Dutta, Rahul Dey, Rituparna Debnath, Sabrina Sultana Rahman, Biswa Prasun Chatterji","doi":"10.1093/lambio/ovag038","DOIUrl":"10.1093/lambio/ovag038","url":null,"abstract":"This study investigates the potential of onion peel waste as a sustainable and cost-effective alternative substrate for xanthan gum production using Xanthomonas campestris NCIM 2961. The results demonstrate that onion peel-based medium consistently produced higher xanthan gum yields compared to the standard medium, achieving a maximum yield of 5.21 g L-1 at optimal conditions (pH 7.0, 30°C, 100 rpm agitation, and a 48 h incubation period), without supplements or pretreatments. FT-IR confirmed the chemical identity and purity of xanthan gum derived from the onion peel-based medium, closely matching commercial food-grade xanthan gum. Utilizing onion peels as a carbon substrate can contribute to waste management in India, a major onion producer, generating tons of onion peel waste every year. This would also align with sustainable development and circular economic goals. However, large-scale production of xanthan gum may be challenging due to factors like variable peel composition, inhibitory compounds, microbial contaminants, and extraction limitations. Future efforts focused on refining pretreatment and purification techniques, engineering Xanthomonas strains for improved waste utilization, and optimizing fermentation conditions using response surface methodology could further boost production efficiency. These findings highlight the viability of onion peel waste as a standalone substrate for cost-effective and sustainable xanthan gum production.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in PCR-Based Detection of Escherichia coli. 基于pcr的大肠杆菌检测研究进展。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-04-05 DOI: 10.1093/lambio/ovag045

Bohan Hu, Chuanjin Cui, Jingdong Bo, Qian Li, Shubao Wang

引用次数: 0

Biotransformation of naproxen by an atoxigenic Bacillus cereus group strain closely related to Bacillus anthracis: linking genomic features to biodegradative activity. 与炭疽芽孢杆菌密切相关的嗜氧蜡样芽孢杆菌群菌株对萘普生的生物转化：将基因组特征与生物降解活性联系起来。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag028

Jessica L Ortega-Balleza, Mailyn S Porras-García, Ana Verónica Martinez-Vázquez, Eyra Ortiz-Pérez, Alma D Paz-González, Gildardo Rivera

{"title":"Biotransformation of naproxen by an atoxigenic Bacillus cereus group strain closely related to Bacillus anthracis: linking genomic features to biodegradative activity.","authors":"Jessica L Ortega-Balleza, Mailyn S Porras-García, Ana Verónica Martinez-Vázquez, Eyra Ortiz-Pérez, Alma D Paz-González, Gildardo Rivera","doi":"10.1093/lambio/ovag028","DOIUrl":"10.1093/lambio/ovag028","url":null,"abstract":"Nonsteroidal anti-inflammatory drugs (NSAIDs), such as naproxen (Nap), are among the most widely consumed medications worldwide. However, wastewater treatments are inefficient at removing them, leading to their persistence in the environment. In this study, bacteria isolated from the contaminated lagoon \"La Escondida\", Reynosa, Mexico, were initially screened for tolerance to pharmaceutical drugs, including Nap. Ten bacterial isolates showed growth in the presence of both Amp (ampicillin) and Nap (100 mg l-1). Isolate P43-LBF was identified as a Bacillus group, showing high genomic similarity (ANI 98.26 %) to Bacillus anthracis but lacking the pOX1 and pOX2 virulence plasmids. It caused a degradation of 67.43 % and 43.78 % (5 and 25 mg l-1, respectively) of Nap in glucose-supplemented minimal medium in seven days. Degradation products, such as 6-O-desmethylnaproxen, were detected by Ultra Performance Liquid Chromatography Mass Spectrometry (UPLC-MS), indicating active biotransformation. Genomic analysis revealed genes associated with xenobiotic degradation, including catE, encoding catechol-2,3-dioxygenase. Additionally, genes associated with virulence and resistance were also identified, which support stress tolerance. Phylogenetic analysis of Bacillus species known for their ability to degrade aromatic compounds supports their potential application in pharmaceutical bioremediation. These findings highlight strain P43-LBF as a promising candidate for pharmaceutical bioremediation.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147290113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of a blend of four Alkalihalobacillus clausii strains (O/C, N/R, SIN, and T) on gut barrier integrity and immune modulation with PPIs: an in vitro SHIME study. 四种克劳氏碱杆菌（O/C、N/R、SIN和T）混合对肠道屏障完整性和PPIs免疫调节的影响：一项体外SHIME研究

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag025

Lynn Verstrepen, Cindy Duysburgh, Mattia Van den Broeck, Marcos Iii Perez, Denis Guyonnet

{"title":"Impact of a blend of four Alkalihalobacillus clausii strains (O/C, N/R, SIN, and T) on gut barrier integrity and immune modulation with PPIs: an in vitro SHIME study.","authors":"Lynn Verstrepen, Cindy Duysburgh, Mattia Van den Broeck, Marcos Iii Perez, Denis Guyonnet","doi":"10.1093/lambio/ovag025","DOIUrl":"10.1093/lambio/ovag025","url":null,"abstract":"Alkalihalobacillus clausii is effective in alleviating PPI-induced dysbiosis; however, its effects on gut barrier function and inflammation remain unclear. This study explored A. clausii (O/C, N/R, SIN, and T) effects on gut barrier function during PPI-induced dysbiosis using Triple-M-SHIME® model. Reactors were supplemented with PPI alone (PPI control); simultaneous PPI + A. clausii (preventive); and PPI for 2 weeks followed by A. clausii (curative). Caco-2/THP1-Blue™ co-cultures were exposed to colonic SHIME® suspensions for 24 h for TEER measurement, followed by lipopolysaccharide stimulation for measurement of cytokine secretion and NF-κB. Preventive treatment did not improve inflammation-induced barrier disruption, but was slightly improved with curative treatment, particularly in the distal colon. Colonic ferments following A. clausii supplementation increased IL-10 secretion and pro-inflammatory signals in the ileum. In the proximal and distal colon, PPI treatment reduced pro-inflammatory markers during and after treatment, while increasing anti-inflammatory cytokines following prolonged incubation. Preventive treatment with A. clausii, reduced pro-inflammatory markers and IL-10, while increasing IL-6 secretion. Curative A. clausii treatment lowered pro-inflammatory markers. Enhanced colonic fermentation by A. clausii thus showed potential in improving gut barrier function and modulating inflammation with complex colon region-specific responses, particularly with curative treatment.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic characterization of Bacillus subtilis Strain BS-Q and its cell-free-supernatant-mediated inhibition of Clostridium perfringens. 枯草芽孢杆菌菌株BS-Q的益生菌特性及其对产气荚膜梭菌的无上清抑制作用。

IF 2.1 4区生物学

Letters in Applied Microbiology Pub Date : 2026-03-06 DOI: 10.1093/lambio/ovag008

Qianqian Dai, Junli Ni, Junying Lu, Weiwei Shi, Xiangjie Chen, Yibin Zhu, Yongle Song, Haiming Cai, Xuhui Lin, Minna Lv, Juan Li, Shenquan Liao, Mingfei Sun, Jianfei Zhang, Xiaohui Zhang, Qianqian Hu, Nanshan Qi

{"title":"Probiotic characterization of Bacillus subtilis Strain BS-Q and its cell-free-supernatant-mediated inhibition of Clostridium perfringens.","authors":"Qianqian Dai, Junli Ni, Junying Lu, Weiwei Shi, Xiangjie Chen, Yibin Zhu, Yongle Song, Haiming Cai, Xuhui Lin, Minna Lv, Juan Li, Shenquan Liao, Mingfei Sun, Jianfei Zhang, Xiaohui Zhang, Qianqian Hu, Nanshan Qi","doi":"10.1093/lambio/ovag008","DOIUrl":"10.1093/lambio/ovag008","url":null,"abstract":"This study characterized Bacillus subtilis (B. subtilis) BS-Q as a novel probiotic candidate against Clostridium perfringens (C. perfringens). Polyphasic analysis, including 16S rRNA gene sequencing (>99% similarity to reference B. subtilis strains), identified the isolate as BS-Q. It exhibited robust growth, and sporulation was observed. Its cell-free supernatant (CFS) demonstrated potent, broad-spectrum inhibition against C. perfringens types A, C, and G with a minimum inhibitory concentration (MIC) of 33.06 ± 0.53 μg/mL. The antibacterial activity was localized to the CFS and showed remarkable stability after exposure to harsh conditions: high temperature (100°C for 30 min), a broad pH range (3.0-11.0), and high salinity (up to 10% NaCl). Protease treatment markedly reduced the activity, confirming the proteinaceous nature of the antimicrobial compounds, which was supported by the presence of unique protein bands (25-35 kDa) in the CFS as revealed by SDS-PAGE. In conclusion, B. subtilis BS-Q produces highly stable, proteinaceous antimicrobials effective against C. perfringens. Its strong anti-clostridial activity and resilience position it as a promising probiotic and a potential antibiotic alternative for controlling necrotic enteritis in poultry.","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0