Journal of Traditional and Complementary Medicine最新文献_第4页

Analyzing mechanisms of Qing Fei Bao Yuan Decoction granules in treating COPD based on LC-MS, network pharmacology and in vivo methods 基于LC-MS、网络药理学和活体方法分析清热保元颗粒治疗慢性阻塞性肺疾病的机制

IF 4.5 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-04-01 DOI: 10.1016/j.jtcme.2024.04.005

Amei Tang, Yang Liu, Guoqiang Guan, Tong Hao, Feng-Di Cao

引用次数: 0

Pulsatilla saponin inhibits the proliferation of keratinocytes and ameliorates imiquimod-induced psoriasis through the NF-κB and STAT3 signaling pathways 白头翁皂苷通过 NF-κB 和 STAT3 信号通路抑制角朊细胞增殖并改善咪喹莫特诱导的银屑病

IF 4.5 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-04-01 DOI: 10.1016/j.jtcme.2024.04.001

Jilang Li, Haixin Qiu, Siyuan Li, Shan Han, Yuming He, Jiangcheng He, Xiang Gao, Jingjing Li, Jianfang Feng, Shilin Yang, Renyikun Yuan, Hongwei Gao

引用次数: 0

A proteasome-dependent inhibition of SIRT-1 by the resveratrol analogue 4,4′-dihydroxy-trans-stilbene 白藜芦醇类似物 4,4′-二羟基-反式-二苯乙烯对蛋白酶体 SIRT-1 的依赖性抑制作用

IF 3.3 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-08 DOI: 10.1016/j.jtcme.2024.03.001

{"title":"A proteasome-dependent inhibition of SIRT-1 by the resveratrol analogue 4,4′-dihydroxy-trans-stilbene","authors":"","doi":"10.1016/j.jtcme.2024.03.001","DOIUrl":"10.1016/j.jtcme.2024.03.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>Resveratrol (RSV), is a stilbene-based compound exerting wide biological properties. Its analogue 4,4′-dihydroxy-<em>trans</em>-stilbene (DHS) has shown improved bioavailability and antiproliferative activity <em>in vitro</em> and <em>in vivo</em>. One of the hypotheses on how resveratrol works is based on SIRT1 activation. Since their strict structural similarities, we have explored a potential interaction between DHS and SIRT1, in comparison with the parental molecule.</p></div><div><h3>Experimental procedure</h3><p>Timing of incubation and concentrations of DHS have been determined using MTT assay in normal human lung fibroblasts. Untreated, DHS- or RSV-treated cells were harvested and analysed by Western Blotting or RT-PCR, in order to evaluate SIRT1 levels/activity and expression, and by Cellular Thermal shift assay (CETSA) to check potential DHS or RSV-SIRT1 interaction. Transfection experiments have been performed with two SIRT1 mutants, based on the potential binding pockets identified by Molecular Docking analysis.</p></div><div><h3>Results and conclusion</h3><p>We unexpectedly found that DHS, but not RSV, exerted a time-dependent inhibitory effect on both SIRT1 protein levels and activity, the latter measured as p53 acetylation. At the mRNA level no significant changes were observed, whereas a proteasome-dependent mechanism was highlighted for the reduction of SIRT1 levels by DHS in experiments performed with the proteasome inhibitor MG132. Bioinformatics analysis suggested a higher affinity of RSV in binding all SIRT1 complexes compared to DHS, except comparable results for complex SIRT1-p53. Nevertheless, both CETSA and SIRT1 mutants transfected in cells did not confirm this interaction. In conclusion, DHS reduces SIRT1 protein level, thereby inhibiting its activity through a proteasome-mediated mechanism.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000208/pdfft?md5=47fe3c459e6cf2bb0ea6bf4959afa54c&pid=1-s2.0-S2225411024000208-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jintiange capsule ameliorates glucocorticoid-induced osteonecrosis of the femoral head in rats by regulating the activity and differentiation of BMSCs 金天戈胶囊通过调节BMSCs的活性和分化改善糖皮质激素诱发的大鼠股骨头坏死

IF 3.3 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-07 DOI: 10.1016/j.jtcme.2024.03.013

{"title":"Jintiange capsule ameliorates glucocorticoid-induced osteonecrosis of the femoral head in rats by regulating the activity and differentiation of BMSCs","authors":"","doi":"10.1016/j.jtcme.2024.03.013","DOIUrl":"10.1016/j.jtcme.2024.03.013","url":null,"abstract":"<div><h3>Background and aim</h3><p>A surplus of glucocorticoids (GC) is a main cause of non-traumatic osteonecrosis of the femoral head (ONFH), and Jintiange (JTG), as one of the traditional Chinese medicines (TCM), also plays an instrumental role in the alleviation of bone loss simultaneously. Therefore, JTG was thought to be able to reverse GC-induced ONFH (GC-ONFH) to a certain extent.</p></div><div><h3>Experimental procedure</h3><p>In vivo, the effect of JTG on trabeculae in the subchondral bone of the femoral head was investigated using micro-computed tomography (micro-CT), TdT-mediated dUTP nick end labeling (TUNEL) and histological staining; in vitro, proliferation, viability, apoptosis, and senescence of purified bone mesenchymal stem cells (BMSCs) were examined to demonstrate the direct impact of JTG on these cells. Meanwhile after using a series of interventions, the function of JTG on BMSC differentiation could be assessed by measuring of osteogenic and adipogenic markers at levels of protein and mRNA.</p></div><div><h3>Results</h3><p>Our final results demonstrated that with the involvement of Wnt/β-catenin pathway, JTG was able to significantly promote osteogenesis, restrain adipogenesis, delay senescence in BMSCs, reduce osteoclast number, weaken apoptosis, and enhance proliferation of osteocytes, all of which could mitigate the progression of subchondral osteonecrosis.</p></div><div><h3>Conclusion</h3><p>According to the results of experiments in vitro and vivo, JTG was deemed to relieve the early GC-ONFH using the prevention of destruction of subchondral bone, which was contributed to regulating the differentiation of BMSCs and the number of osteoclasts.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000312/pdfft?md5=eb30723a9cbb02e155129ca1b2543747&pid=1-s2.0-S2225411024000312-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140087971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase 1 clinical trial evaluating safety, bioavailability, and gut microbiome with a combination of curcumin and ursolic acid in lipid enhanced capsules 评估姜黄素和熊果酸脂质强化胶囊组合的安全性、生物利用率和肠道微生物组的 1 期临床试验

IF 3.3 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-07 DOI: 10.1016/j.jtcme.2024.03.002

{"title":"Phase 1 clinical trial evaluating safety, bioavailability, and gut microbiome with a combination of curcumin and ursolic acid in lipid enhanced capsules","authors":"","doi":"10.1016/j.jtcme.2024.03.002","DOIUrl":"10.1016/j.jtcme.2024.03.002","url":null,"abstract":"<div><p>As screening strategies employ better biomarkers and genetics to identify individuals at an increased risk of prostate cancer, there are currently no chemotherapeutic prevention strategies. With any chemoprevention strategy, the population will be younger and healthier; therefore, they will be less tolerant of side effects. This study translated findings from screening a natural product library and pre-clinical evaluation of curcumin (CURC) in combination with ursolic acid (UA) in prostate cancer models. After manufacturing capsules for each compound, 18 subjects were enrolled. The study used a 3 × 3 phase 1 clinical trial to evaluate CURC (1200 mg/day) and UA (300 mg/day) alone and in combination over a 2-week period with endpoints of safety, bioavailability, and microbiome alterations. After enrolling six subjects in each arm, we found no grade 3 or 4 events and only minor changes in the safety laboratory values. In the pooled analysis of groups, we noted a statistically significant difference between median serum levels of UA when administered alone vs administered in the combination (2.7 ng/mL vs 43.8 ng/mL, p = 0.03). Individuals receiving the combination also had a favorable impact on gut microbiome status and a reduction in “microbiome score” predictive of prostate cancer risk.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S222541102400021X/pdfft?md5=d678b810e7bfbd0337e3fd51f0011836&pid=1-s2.0-S222541102400021X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical profiling and mechanisms of Agarikon pill in a rat model of cigarette smoke-induced chronic obstructive pulmonary disease 阿加瑞康丸在香烟烟雾诱发慢性阻塞性肺病大鼠模型中的化学分析和作用机制

IF 3.3 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-06 DOI: 10.1016/j.jtcme.2024.03.006

{"title":"Chemical profiling and mechanisms of Agarikon pill in a rat model of cigarette smoke-induced chronic obstructive pulmonary disease","authors":"","doi":"10.1016/j.jtcme.2024.03.006","DOIUrl":"10.1016/j.jtcme.2024.03.006","url":null,"abstract":"<div><h3>Background and aim</h3><p>Agarikon pill (AGKP), a traditional Chinese herbal formula, and has been used for chronic obstructive pulmonary disease (COPD) treatment clinically. However, the active components and exact pharmacological mechanisms are still unclear. We aimed to investigate the therapeutic effects and mechanisms of AGKP on COPD and identify the chemical constituents and active compounds.</p></div><div><h3>Experimental procedure</h3><p>The chemical components of AGKP were identified by ultrahigh-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS). Network pharmacology analysis was performed to uncover the potential mechanism of AGKP. The efficiencies and mechanisms of AGKP were further confirmed in COPD animal models.</p></div><div><h3>Results and conclusion</h3><p>Ninety compounds from AGKP, such as flavonoids, triterpenoids, saponins, anthracenes, derivatives, phenyl propionic acid, and other organic acids, were identified in our study. AGKP improved lung function and pathological changes in COPD model rats. Additionally, inflammatory cell infiltration and proinflammatory cytokine levels were markedly reduced in COPD rats administered AGKP. Network pharmacology analysis showed that the inflammatory response is the crucial mechanism by which AGKP exerts therapeutic effects on COPD rats. WB and PCR data indicated that AGKP attenuated the inflammatory response in COPD model rats. AGKP reduces the pulmonary inflammatory response through the PI3K/AKT and MAPK TLR/NF-κB signaling pathways and exerts therapeutic effects via inhibition of inflammation and mucus hypersecretion on COPD model rats.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000257/pdfft?md5=cee256381934180d5a2cc9c49d4be55b&pid=1-s2.0-S2225411024000257-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring hepatic fibrosis screening via deep learning analysis of tongue images 通过舌头图像的深度学习分析探索肝纤维化筛查

IF 3.3 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-06 DOI: 10.1016/j.jtcme.2024.03.010

{"title":"Exploring hepatic fibrosis screening via deep learning analysis of tongue images","authors":"","doi":"10.1016/j.jtcme.2024.03.010","DOIUrl":"10.1016/j.jtcme.2024.03.010","url":null,"abstract":"<div><h3>Background</h3><p>Tongue inspection, an essential diagnostic method in Traditional Chinese Medicine (TCM), has the potential for early-stage disease screening. This study aimed to evaluate the effectiveness of deep learning-based analysis of tongue images for hepatic fibrosis screening.</p></div><div><h3>Methods</h3><p>A total of 1083 tongue images were collected from 741 patients and divided into training, validation, and test sets. DenseNet-201, a convolutional neural network, was employed to train the AI model using these tongue images. The predictive performance of AI was assessed and compared with that of FIB-4, using real-time two-dimensional shear wave elastography as the reference standard.</p></div><div><h3>Results</h3><p>The proposed AI model achieved an accuracy of 0.845 (95% CI: 0.79–0.90) and 0.814 (95% CI: 0.76–0.87) in the validation and test sets, respectively, with negative predictive values (NPVs) exceeding 90% in both sets. The AI model outperformed FIB-4 in all aspects, and when combined with FIB-4, the NPV reached 94.4%.</p></div><div><h3>Conclusion</h3><p>Tongue inspection, with the assistance of AI, could serve as a first-line screening method for hepatic fibrosis.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000294/pdfft?md5=95254b587bfa96b8670590c12fc58de5&pid=1-s2.0-S2225411024000294-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140271350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chinese herbal medicine may reduce major adverse cardiovascular events in patients with dialysis hypotension: A taiwan nationwide cohort study 中药可减少透析低血压患者的主要心血管不良事件：一项台湾全国性队列研究

IF 3.3 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-06 DOI: 10.1016/j.jtcme.2024.03.009

{"title":"Chinese herbal medicine may reduce major adverse cardiovascular events in patients with dialysis hypotension: A taiwan nationwide cohort study","authors":"","doi":"10.1016/j.jtcme.2024.03.009","DOIUrl":"10.1016/j.jtcme.2024.03.009","url":null,"abstract":"<div><h3>Background</h3><p>The association between Chinese herbal medicine (CHM) and the risk of developing major adverse cardiovascular events (MACEs) in patients with dialysis hypotension is unclear and has not yet been investigated. This study aimed to determine whether CMH intervention could reduce the risk of MACEs in patients with dialysis hypotension.</p></div><div><h3>Methods</h3><p>The study data from the Taiwan National Health Insurance Research Database were analyzed to clarify this association. For this study, a case-control design with a cohort of patients who received hemodialysis (HD) from 2008 to 2018, 20 295 HD patients who had received blood pressure (BP) raising drugs were identified. After 1:1 frequency-matching, 730 patients were identified as CHM users and CHM non-users. Vascular access revision/reconstruction and MACEs were observed as the main outcomes during the follow-up period.</p></div><div><h3>Results</h3><p>The occurrence of vascular access revision/reconstruction in HD patients receiving BP raising drugs was associated with a 0.34-fold lower risk in CHM users than in CHM non-users [adjusted hazard ratio (aHR) = 0.34, 95% confidence interval (CI) = 0.26, 0.45]. The occurrences of MACEs in HD patients receiving BP raising drugs was associated with a 0.41-fold lower risk in CHM users than in CHM non-users (aHR = 0.41, 95% CI = 0.33, 0.51). A markedly predominant effect was observed in those receiving CHM for more than 180 days (aHR = 0.32; 95% CI = 0.22, 0.45).</p></div><div><h3>Conclusion</h3><p>The findings revealed lower vascular access dysfunction and MACEs risk correlated with the use of CHM treatment among HD patients who received BP raising drugs.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000282/pdfft?md5=5141fff4055070402a9c46f7307c0e52&pid=1-s2.0-S2225411024000282-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140267936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zhilong Huoxue Tongyu capsule protects against atherosclerosis by suppressing End MT via modulating Hippo/YAP signaling pathway 芝龙藿香通脉胶囊通过调节 Hippo/YAP 信号通路抑制 End MT 防止动脉粥样硬化

IF 4.5 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-01 DOI: 10.1016/j.jtcme.2024.03.015

Yanan Zhou, Hong Wang, Tao Bi, P. Liang, Xinyue Liu, Hongping Shen, Qin Sun, Gang Luo, Ping Liu, Sijin Yang, Wei-Ming Ren

引用次数: 0

Antcin-H, a natural triterpene derived from Antrodia cinnamomea, ameliorates dextran sulfate sodium-induced colitis in mice by inhibiting the NLRP3 inflammasome Antcin-H 是一种从桂枝中提取的天然三萜类化合物，可通过抑制 NLRP3 炎性体改善右旋糖酐硫酸钠诱导的小鼠结肠炎

IF 4.5 3区医学

Journal of Traditional and Complementary Medicine Pub Date : 2024-03-01 DOI: 10.1016/j.jtcme.2024.03.016

Wei-Ting Wong, Lan-hui Li, Hsiao-Wen Chiu, M. Menon, Hsien-Ta Hsu, Wen-Yu Lin, Chun-Hsien Wu, Chen-Lung Ho, Kuo-Feng Hua

引用次数: 0