Journal of Traditional and Complementary Medicine最新文献

{"title":"Gut-joint axis: Oral Probiotic ameliorates Osteoarthritis","authors":"Uzma Amin ,&nbsp;Rong Jiang ,&nbsp;Shahid Masood Raza ,&nbsp;Mengtian Fan ,&nbsp;Li Liang ,&nbsp;Naibo Feng ,&nbsp;Xiaoli Li ,&nbsp;Yuyou Yang ,&nbsp;Fengjin Guo","doi":"10.1016/j.jtcme.2023.06.002","DOIUrl":"10.1016/j.jtcme.2023.06.002","url":null,"abstract":"<div><p>Osteoarthritis (OA) etiology is multifactorial, and its prevalence is growing globally. The Gut microbiota shapes our immune system and impacts all aspects of health and disease. The idea of utilizing probiotics to treat different conditions prevails. Concerning musculoskeletal illness and health, current data lack the link to understand the interactions between the host and microbiome. We report that <em>S. thermophilus, L. pentosus</em> (as probiotics), and γ-aminobutyric acid (GABA) harbour against osteoarthritis in vivo and alleviate IL-1β induced changes in chondrocytes in vitro. We examined the increased GABA concentration in mice's serum and small intestine content followed by bacterial treatment. The treatment inhibited the catabolism of cartilage and rescued mice joints from degradation. Furthermore, the anabolic markers upregulated and decreased inflammatory markers in mice knee joints and chondrocytes. This study is the first to represent GABA's chondrogenic and chondroprotective effects on joints and human chondrocytes. This data provides a foundation for future studies to elucidate the role of GABA in regulating chondrocyte cell proliferation. These findings opened future horizons to understanding the gut-joint axis and OA treatment. Thus, probiotic/GABA therapy shields OA joints in mice and could at least serve as adjuvant therapy to treat osteoarthritis.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 26-39"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000743/pdfft?md5=17795c8fb1abc93eb8a29e28722a4a35&pid=1-s2.0-S2225411023000743-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43122981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhoushi Qi Ling decoction inhibits the progression of castration-resistant prostate cancer in vivo by regulating macrophage infiltration via IL6-STAT3 signaling","authors":"Hongwen Cao ,&nbsp;Dan Wang ,&nbsp;Renjie Gao ,&nbsp;Lei Chen,&nbsp;Yigeng Feng,&nbsp;Peng Sun","doi":"10.1016/j.jtcme.2023.05.005","DOIUrl":"10.1016/j.jtcme.2023.05.005","url":null,"abstract":"<div><h3>Background and aim</h3><p>Prostate cancer is a leading malignant tumor in men, associated with a high rate of mortality. Androgen deprivation therapy is commonly used to treat prostate cancer, which contributes to the progression of castration-resistant prostate cancer (CRPC). The current therapy has a low survival rate in patients with CRPC. Our study aims to develop a novel effective approach for CRPC treatment and improve survival benefits.</p></div><div><h3>Experimental procedure</h3><p>CRPC cell line PC-3-Luc expressing luciferase and the CRPC cell line PC-3-IL6-Luc stably overexpressing IL-6 were used to establish the xenograft tumor mouse model. The tumor was monitored weekly using Bioluminescence imaging. Infiltrated macrophages were quantified by fluorescence-activated cell sorting using flow cytometry. IL6 mRNA level was determined using quantitative real-time PCR. The protein levels of total STAT3 and phosphorylated STAT3 were determined using Western blot.</p></div><div><h3>Results and conclusion</h3><p>Zhoushi Qi Ling decoction (ZQD) treatment significantly reduced PC3 the xenograft tumor progression and the number of infiltrated macrophages when compared with saline treatment. IL6 mRNA level was remarkedly suppressed by ZQD treatment. Notably, the protein level of phosphorylated STAT3 was significantly decreased in PC3 the xenograft tumor treated with ZQD compared to saline treatment. Our findings demonstrated that ZQD treatment significantly reduced the progression of prostate cancer, evidenced by the reduced population of infiltrated macrophages and the inhibition of the IL6/STAT3 pathway.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 19-25"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000603/pdfft?md5=c5400407e9f8415585b69fef4c69703e&pid=1-s2.0-S2225411023000603-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49138082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture on Baihui (DU20) and Xuehai (SP10) acupoints alleviates psoriatic inflammation by regulating neurotransmitter substance P- Neurokinin-1 receptor signaling","authors":"Cong Qi ,&nbsp;Fang Feng ,&nbsp;JianNing Guo ,&nbsp;Yu Liu ,&nbsp;XiaoYao Guo ,&nbsp;YuJiao Meng ,&nbsp;TingTing Di ,&nbsp;XueQing Hu ,&nbsp;Yazhuo Wang ,&nbsp;Ning Zhao ,&nbsp;XiaWei Zhang ,&nbsp;Yan Wang ,&nbsp;Jingxia Zhao ,&nbsp;Ping Li","doi":"10.1016/j.jtcme.2023.07.005","DOIUrl":"10.1016/j.jtcme.2023.07.005","url":null,"abstract":"<div><h3>Background</h3><p>At present, acupuncture-related practices have been widely used to treat psoriasis. In our study, we investigated the effect and explored the mechanism of electroacupuncture (EA) on acupoints Baihui (DU20) and Xuehai (SP10) for the treatment of psoriasis.</p></div><div><h3>Methods</h3><p>Imiquimod-induced psoriasis-like mouse model was used in this study. Mice were treated with electroacupuncture at DU20 and SP10 (depth of 2–3 mm, frequency of 2/15 Hz, intensity of 0.5–1.0 mA, 10 min/day). The severity of psoriasis-like lesions for each group was assessed. In addition, histological analysis of the lesions were performed. The levels of inflammatory cytokines were determined using Elisa. The expression levels of Substance P (SP) and NK1R were measured using Western blotting. In addition, NK1R inhibitor was administrated to evaluate the target of electroacupuncture in our mouse model.</p></div><div><h3>Results</h3><p>Electroacupuncture significantly alleviated IMQ-induced skin lesions and epidermal thickness, accompanied with reduced keratinocyte proliferation, CD3⁺, CD4⁺, and CD8⁺ T cells infiltration. The reduced levels of inflammatory cytokines was observed after electroacupuncture treatment. In addition, electroacupuncture inhibited the expression levels of SP and NK1R. NK1R inhibitor could ameliorate lesional symptoms and suppress epidermal thickening and CD3⁺, CD4⁺, and CD8 + T cell infiltration.</p></div><div><h3>Conclusions</h3><p>Electroacupuncture relieved psoriasis-like inflammation and T cell infiltration. This therapeutic action was likely mediated by the modulation of Substance P and its receptor NK1R.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 91-100"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000792/pdfft?md5=1912b64497714134f2b9a21e7986a019&pid=1-s2.0-S2225411023000792-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41519230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gracilaria chorda subcritical-water extracts as ameliorant of insulin resistance induced by high-glucose in zebrafish and dexamethasone in L6 myotubes","authors":"Laxmi Sen Thakuri ,&nbsp;Chul Min Park ,&nbsp;Jin Woo Park ,&nbsp;Dong Young Rhyu","doi":"10.1016/j.jtcme.2023.07.006","DOIUrl":"10.1016/j.jtcme.2023.07.006","url":null,"abstract":"<div><h3>Background and aim</h3><p>Insulin resistance (IR) is a pathological condition in which cells fail to respond normally to insulin. Loss of insulin sensitivity disrupts glucose homeostasis and elevates the risk of developing the metabolic syndrome that includes Type 2 diabetes. This study assesses the effect on subcritical-water extract of <em>Gracilaria chorda</em> (GC) at 210 °C (GCSW210) in IR induction models of high glucose (HG)-induced zebrafish larvae and dexamethasone (DEX)-induced L6 myotubes.</p></div><div><h3>Experimental procedure</h3><p>The dose of HG and DEX for IR induction in zebrafish larvae and L6 myotubes was 130 mM or 0.5 μM. The capacity of glucose uptake was quantified by fluorescence staining or intensity. In addition, the activation of protein and mRNA expressions for insulin signaling (insulin-dependent or independent pathways) was measured.</p></div><div><h3>Results and conclusion</h3><p>Exposure of zebrafish larvae to HG significantly reduced the intracellular glucose uptake with dose-dependnet manner compared to control. However, the group treated with GCSW210 significantly averted HG levels like the insulin-treated group, and significantly up- or down-regulated the mRNA expressions related to insulin production (<em>insα</em>) and insulin signaling pathways. Moreover, the treatment with GCSW210 effectively regulated the protein expression of PI3K/AKT, AMPK, and GLUT4 involved in the action of insulin in IR models of L6 myotubes compared to DEX-treated control. Our data indicate that GCSW210 stimulates activation of PI3K/AKT and AMPK pathways to attenuate the development of IR induced by HG in zebrafish and DEX in L6 myotubes. In conclusion, GCSW210 is a potential agent for alleviating various diseases associated with the insulin resistance.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 1","pages":"Pages 82-90"},"PeriodicalIF":4.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000810/pdfft?md5=cffa21840dfcf96023d4538c6a69cb26&pid=1-s2.0-S2225411023000810-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characterization of traditional Chinese medicine natures and flavors using network pharmacology integrated strategy","authors":"Hong Wang,&nbsp;Wenfeng Wei,&nbsp;Jing Liu,&nbsp;Shuang Zhang,&nbsp;Yunli Zhao,&nbsp;Zhiguo Yu","doi":"10.1016/j.jtcme.2023.12.004","DOIUrl":"10.1016/j.jtcme.2023.12.004","url":null,"abstract":"<div><h3>Background and aim</h3><p>Due to the complexity of TCM ingredients and medication compatibility, TCM cannot be used like chemical medicines. The theory of “Four Natures and five Flavors” provides a theoretical basis for the use of TCM. “Four Natures and five Flavors” are originated from pharmacological rules based on clinical practices. Whereas, How to describe and characterize “Natures”(Warm, Hot, Cold and Cool) and “Flavors” (Pungent, Sour, Sweet, Bitter and Salty) scientifically remain the issue that needs to be solved. The aim of this study is to establish the TCM characterization models based on the integrated pharmacology network strategy and provide a deeper understanding of TCM theory.</p></div><div><h3>Experimental procedure</h3><p>Five “Pungent-Neutral”, nine “Sweet-Neutral and nine “Bitter-Neutral” TCMs were selected to characterize the “Flavors” (Pungent, Sweet and Bitter). Nine “Pungent-Warm” and nine “Bitter-Cold” TCMs were selected to characterize the “Natures” (Warm and Cold). The screened chemical ingredients were analyzed by classification and the screened characteristics targets were analyzed by GO and KEGG enrichment analysis.</p></div><div><h3>Results and conclusion</h3><p>In the “Pungent” group, flavonoids are the most. “Pungent” may have immune-regulatory effects and potential anticancer activity. In the “Sweet” group, isoflavones are the most. “Sweet” are related to effectively invigorate health. Fatty acids in the “Warm” group are the most. Flavonoids in the “Cold’ group are far more than other components. “Warm” and “Cold” are both related to fatty acid and energy metabolism.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 343-349"},"PeriodicalIF":4.5,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001384/pdfft?md5=9062875cd7de154665e6dc5e2908a99b&pid=1-s2.0-S2225411023001384-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139194919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcuma longa alone and in combination with pioglitazone attenuates paraquat-induced lung injury in rats through improving inflammation, oxidative stress and fibrosis","authors":"Seyedeh Zahra Ghasemi, Sima Beigoli, S. Behrouz, Z. Gholamnezhad, Nema Mohammadian Roshan, M. Boskabady","doi":"10.1016/j.jtcme.2023.12.001","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.12.001","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"12 10","pages":""},"PeriodicalIF":4.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A diet enriched with Pistacia atlantica fruits improves the female rats' reproductive system","authors":"Manijeh Dogani ,&nbsp;Nayere Askari ,&nbsp;Ali Kalantari Hesari","doi":"10.1016/j.jtcme.2023.09.004","DOIUrl":"10.1016/j.jtcme.2023.09.004","url":null,"abstract":"<div><h3>Background and aim</h3><p>Baneh (<em>Pistacia atlantica</em>) is a plant species that is commonly consumed as food and has a long-standing traditional use as a sexual enhancer. Despite its widespread use, a limited amount of academic and scientific literature is available regarding its potential impact on the reproductive system. The present research aimed to study the effect of a diet enriched with Baneh on the female rats’ reproductive system.</p></div><div><h3>Experimental procedure</h3><p>Three groups of rats (n = 8) were subjected to the intended diet for six weeks. Subsequently, their histomorphometric parameters, sex hormone levels, as well as the expression of oxytocin (OXT) and oxytocin receptor (OXTR) genes were measured. The rats’ serum vitamin D, zinc, and lipid profiles were also evaluated.</p></div><div><h3>Results and conclusion</h3><p>Results revealed that compared to the normal food, the diet containing 20 % Baneh significantly increased the progesterone and estradiol levels three and two times, respectively. It decreased the total body weight while increasing the ratio of ovary weight to the body weight. Furthermore, the Baneh-enriched diet raised HDL, zinc, and vitamin D levels, though it reduced the LDL and TG levels by 15 μg/dl and 24 μg/dl, respectively, and the concentration of ovary malondialdehyde decreased by 50 % in the treated group. Also, the diet increased the follicle graph, corpus luteum, the thickness of the epithelium, the number of endometrial glands, and the expression of both <em>OXT</em> and <em>OXTR</em> genes. Our findings suggested that <em>P. atlantica</em> could considerably improve the female sex hormone levels and their reproductive system.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 335-342"},"PeriodicalIF":4.5,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001013/pdfft?md5=c89325b06fbc02c4920852738ea6fa25&pid=1-s2.0-S2225411023001013-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139305238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating single-cell and spatial transcriptomics to elucidate the crosstalk between cancer-associated fibroblasts and cancer cells in hepatocellular carcinoma with spleen-deficiency syndrome","authors":"Qiuxia Chen ,&nbsp;Jin Luo ,&nbsp;Jiahui Liu ,&nbsp;He Yu ,&nbsp;Meiling Zhou ,&nbsp;Ling Yu ,&nbsp;Yan Chen ,&nbsp;Shijun Zhang ,&nbsp;Zhuomao Mo","doi":"10.1016/j.jtcme.2023.11.008","DOIUrl":"10.1016/j.jtcme.2023.11.008","url":null,"abstract":"<div><h3>Background and aim</h3><p>Most patients with hepatocellular carcinoma (HCC) in China have been diagnosed with spleen deficiency syndrome (SDS), which accelerates the progression of HCC by disrupting the tumor microenvironment homeostasis. This study aimed to investigate the intercellular crosstalk in HCC with SDS.</p></div><div><h3>Experimental procedure</h3><p>An HCC-SDS mouse model was established using orthotopic HCC transplantation based on reserpine-induced SDS. Single-cell data analysis and cancer cell prediction were conducted using Seurat and CopyKAT package, respectively. Intercellular interactions were explored using CellPhoneDB and CellChat and subsequently validated using co-culture assays, ELISA and histological staining. We performed pathway activity analysis using gene set variation analysis and the Seurat package. The extracellular matrix (ECM) remodeling was assessed using a gel contraction assay, atomic force microscopy, and Sirius red staining. The deconvolution of the spatial transcriptomics data using the “CARD” package based on single-cell data.</p></div><div><h3>Results and conclusion</h3><p>We successfully established the HCC-SDS mouse model. Twenty-nine clusters were identified. The interactions between cancer cells and cancer-associated fibroblasts (CAFs) were significantly enhanced via platelet-derived growth factor (PDGF) signaling in HCC-SDS. CAFs recruited in HCC-SDS lead to ECM remodeling and the activation of TGF-β signaling pathway. Deconvolution of the spatial transcriptome data revealed that CAFs physically surround cancer cells in HCC-SDS. This study reveals that the crosstalk of CAFs-cancer cells is crucial for the tumor-promoting effect of SDS. CAFs recruited by HCC via PDGFA may lead to ECM remodeling through activation of the TGF-β pathway, thereby forming a physical barrier to block immune cell infiltration under SDS.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 321-334"},"PeriodicalIF":4.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001220/pdfft?md5=2ef88edfa3b4522b4edf258637a5fb64&pid=1-s2.0-S2225411023001220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139292618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qufeng tongluo decoction decreased proteinuria in diabetic mice by protecting podocytes via promoting autophagy","authors":"Boran Ni ,&nbsp;Yao Xiao ,&nbsp;Ruojun Wei ,&nbsp;Weijing Liu ,&nbsp;Liwei Zhu ,&nbsp;Yifan Liu ,&nbsp;Zhichao Ruan ,&nbsp;Jiamu Li ,&nbsp;Shidong Wang ,&nbsp;Jinxi Zhao ,&nbsp;Weijun Huang","doi":"10.1016/j.jtcme.2023.11.007","DOIUrl":"10.1016/j.jtcme.2023.11.007","url":null,"abstract":"<div><h3>Background</h3><p>Diabetic kidney disease (DKD) is one of diabetic complications, which has become the leading cause of end-stage kidney disease. In addition to angiotensin-converting enzyme inhibitor/angiotensin II receptor blocker(ACEI/ARB) and sodium-glucose cotransporter-2 inhibitor (SGLT2i), traditional Chinese medicine (TCM) is an effective alternative treatment for DKD. In this study, the effect of Qufeng Tongluo (QFTL) decoction in decreasing proteinuria has been observed and its mechanism has been explored based on autophagy regulation in podocyte.</p></div><div><h3>Methods</h3><p>In vivo study, db/db mice were used as diabetes model and db/m mice as blank control. Db/db mice were treated with QFTL decoction, rapamycin, QFTL + 3-Methyladenine (3-MA), trehalose, chloroquine (CQ) and QFTL + CQ. Mice urinary albumin/creatinine (UACR), nephrin and autophagy related proteins (LC3 and p62) in kidney tissue were detected after intervention of 9 weeks. Transcriptomics was operated with the kidney tissue from model group and QFTL group. In vitro study, mouse podocyte clone-5 (MPC-5) cells were stimulated with hyperglycemic media (30 mmol/L glucose) or cultured with normal media. High-glucose-stimulated MPC-5 cells were treated with QFTL freeze-drying powder, rapamycin, CQ, trehalose, QFTL+3-MA and QFTL + CQ. Cytoskeletal actin, nephrin, ATG-5, ATG-7, Beclin-1, cathepsin L and cathepsin B were assessed. mRFP-GFP-LC3 was established by stubRFP-sensGFP-LC3 lentivirus transfection.</p></div><div><h3>Results</h3><p>QFTL decoction decreased the UACR and increased the nephrin level in kidney tissue and high-glucose-stimulated podocytes. Autophagy inhibitors, including 3-MA and chloroquine blocked the effects of QFTL decoction. Further study showed that QFTL decoction increased the LC3 expression and relieved p62 accumulation in podocytes of db/db mice. In high-glucose-stimulated MPC-5 cells, QFTL decoction rescued the inhibited LC3 and promoted the expression of ATG-5, ATG-7, and Beclin-1, while had no effect on the activity of cathepsin L and cathepsin B. Results of transcriptomics also showed that 51 autophagy related genes were regulated by QFTL decoction, including the genes of ATG10, SCOC, ATG4C, AMPK catalytic subunit, PI3K catalytic subunit, ATG3 and DRAM2.</p></div><div><h3>Conclusion</h3><p>QFTL decoction decreased proteinuria and protected podocytes in db/db mice by regulating autophagy.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 312-320"},"PeriodicalIF":4.5,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001219/pdfft?md5=d07f00c2512f8ebe69e9f4c1b19adf52&pid=1-s2.0-S2225411023001219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139299659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral pulse harmonic analysis and its clinical application: A systematic review","authors":"Kun-Yuan Hsiao ,&nbsp;Jiunn-Horng Kang ,&nbsp;Yeong-Shing Wu ,&nbsp;Hen-Hong Chang ,&nbsp;Cheng-Ta Yang","doi":"10.1016/j.jtcme.2023.11.006","DOIUrl":"10.1016/j.jtcme.2023.11.006","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;p&gt;Pulse harmonic analysis is a quantitative and objective methodology within traditional Chinese medicine (TCM) used to evaluate pulse characteristics. However, interpreting pulse wave data is challenging due to its inherent complexity. This study aims to provide a comprehensive review and comparison of existing human pulse wave harmonic analysis methods to elucidate their patterns and characteristics.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;A systematic review of clinical research reports published from 1990 to 2021 was conducted, focusing on variations in harmonic characteristics across different medical conditions and physiological states. Keyword searches included terms related to analysis methods (e.g., \"Pulse Spectrum,\" \"harmonic analysis,\" \"harmonic index\") and measured indicators (e.g., \"vascular response,\" \"PPG,\" \"Photoplethysmography,\" \"aortic,\" \"arterial,\" \"blood pressure\"). Supplementary research using PubMed's Mesh terms specifically targeted \"Pulse wave analysis\" within the methods and statistical analysis domain. Articles were filtered based on predefined criteria, including human participants and research related to pulse pressure or vascular volume changes. Conference papers, animal studies, and irrelevant research were excluded, with literature evaluation scales selected based on the retrieved research reports.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Initially, 6487 research reports were identified, and after screening, 50 reports were included in the review. The analysis revealed that low-frequency harmonics increase following vigorous activity or sympathetic excitation but decrease during rest or parasympathetic excitation. Cardiovascular patients exhibited elevated first harmonics associated with the liver meridian, while diabetes patients displayed weakened third harmonics related to the spleen meridian. Liver dysfunction was linked to changes in the first harmonic, and cancer patients showed signs of liver and kidney yin deficiency in the first and second harmonics. These findings underscore the potential of harmonic analysis for TCM disease diagnosis and organ assessment. Moreover, individuals with conditions such as liver dysfunction, cancer, and gynecological disorders displayed distinct intensity patterns across harmonics one through ten compared to healthy controls, albeit with some variations. Heterogeneity in these studies mainly stemmed from differences in measurement methods and study populations. Additionally, research suggested that factors like blood circulation and cognitive activity influenced harmonic intensity.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;p&gt;In summary, this report consolidates prior research on pulse wave harmonics analysis, revealing unique patterns associated with various physiological conditions. Despite limitations, such as limited sample sizes in previous studies, the observed associations between physiological states and harmonics hold promise for potential clinical applications. Th","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 223-236"},"PeriodicalIF":4.5,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001207/pdfft?md5=30babb8588217a198dc1d70c2df3ce04&pid=1-s2.0-S2225411023001207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139296191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}