Journal of Traditional and Complementary Medicine最新文献

{"title":"The chemical composition of Diwu YangGan capsule and its potential inhibitory roles on hepatocellular carcinoma by microarray-based transcriptomics","authors":"Qingxin Shi ,&nbsp;Jiangcheng He ,&nbsp;Guangya Chen ,&nbsp;Jinlin Xu ,&nbsp;Zhaoxiang Zeng ,&nbsp;Xueyan Zhao ,&nbsp;Binbin Zhao ,&nbsp;Xiang Gao ,&nbsp;Zhihua Ye ,&nbsp;Mingzhong Xiao ,&nbsp;Hanmin Li","doi":"10.1016/j.jtcme.2023.12.002","DOIUrl":"10.1016/j.jtcme.2023.12.002","url":null,"abstract":"<div><p>The Traditional Chinese Medicine compound preparation known as Diwu Yanggan capsule (DWYG) can effectively hinder the onset and progression of hepatocellular carcinoma (HCC), which is recognized worldwide as a significant contributor to fatalities associated with cancer. Nevertheless, the precise mechanisms implicated have remained ambiguous. In present study, the model of HCC was set up by the 2-acetylaminofluorene (2-AAF)/partial hepatectomy (PH) in rats. To confirm the differentially expressed genes (DEGs) identified in the microarray analysis, real-time quantitative reverse transcription PCR (qRT-PCR) was conducted. In the meantime, the liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS) was employed to characterize the component profile of DWYG. Consequently, the DWYG treatment exhibited the ability to reverse 51 variation genes induced by 2-AAF/PH. Additionally, there was an overlap of 54 variation genes between the normal and model groups. Upon conducting RT-qPCR analysis, it was observed that the expression levels of all genes were increased by 2-AAF/PH and subsequently reversed after DWYG treatment. Notably, the fold change of expression levels for all genes was below 0.5, with 3 genes falling below 0.25. Moreover, an investigation was conducted to determine the signaling pathway that was activated/inhibited in the HCC group and subsequently reversed in the DWYG group. Moreover, the component profile of DWYG encompassed a comprehensive compilation of 206 compounds that were identified or characterized. The findings of this study elucidated the potential alleviative mechanisms of DWYG in the context of HCC, thereby holding significant implications for its future clinical utilization and widespread adoption.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 381-390"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001360/pdfft?md5=bfa76b9673e97a298fbe5a8c4cb54ab9&pid=1-s2.0-S2225411023001360-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture improves articular microcirculation and attenuates cartilage hypoxia in a male rabbit model of knee osteoarthritis","authors":"Ma Weiwei ,&nbsp;Du Mei ,&nbsp;Lu Juan ,&nbsp;Xing Longfei ,&nbsp;Chen Xilin ,&nbsp;Hu Tingyao ,&nbsp;Zhu Wenting ,&nbsp;Guo Changqing","doi":"10.1016/j.jtcme.2024.01.002","DOIUrl":"10.1016/j.jtcme.2024.01.002","url":null,"abstract":"<div><h3>Background and aim</h3><p>Hypoxia of the cartilage has been considered as a potential pathogenic factor in knee osteoarthritis (KOA). Studies have shown that impaired blood perfusion of joint leads to cartilage hypoxia. Electroacupuncture (EA) has proven effects on pain relief and improving microcirculation. This study aimed to explore the effect of EA on articular microcirculation and cartilage anoxic and the underlying mechanisms.</p></div><div><h3>Procedures</h3><p>Videman's method was used for 6 weeks to establish the KOA model. EA intervention was performed in four points around the knee for 3 weeks after KOA modeling. The Lequesne MG score was used to assess ethology. We recorded the oxygen tension of synovial fluid and the synovial microcirculation in vivo. HE-staining was used to assess cartilage morphology, and immunohistochemistry (IHC), Western blotting, and RT-PCR were used to assess expression of the major glycolytic enzymes glucosetransporter1 (GLUT1), pyruvate kinase M2(PKM2), and lactate dehydrogenase A (LDHA). Enzyme-linked immunosorbent assay (Elisa) was used to detect lactate content.</p></div><div><h3>Results and conclusion</h3><p>There was a significant decrease in Lequesne MG score and improvement in Mankin score after EA intervention (P &lt; 0.01), a significant increase in synovial microcirculation (P &lt; 0.05) and synovial fluid oxygen tension (P &lt; 0.01), and there was significant decrease in the expression of GLUT1, PKM2 and LDHA (P &lt; 0.01) and lactate (P &lt; 0.05). This study suggested that EA ameliorate cartilage hypoxia and regulate glycolytic metabolism in chondrocytes in KOA model rabbits by improving articular microcirculation and oxygen tension.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 414-423"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000026/pdfft?md5=fc573f1adf9c6cdfa3fad8fda1b0460f&pid=1-s2.0-S2225411024000026-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139394091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sophorae tonkinensis radix polysaccharide attenuates acetaminophen-induced liver injury by regulating the miR-140-5p-related antioxidant mechanism","authors":"Liangliang Cai ,&nbsp;Lixing Xu ,&nbsp;Kai Shen ,&nbsp;Qin Wang ,&nbsp;Ronghua Ni ,&nbsp;Xin Xu ,&nbsp;Xiaofei Ma","doi":"10.1016/j.jtcme.2024.01.006","DOIUrl":"10.1016/j.jtcme.2024.01.006","url":null,"abstract":"<div><p>STRP1, a polysaccharide active ingredient isolated from the traditional Chinese medicine <em>Sophorae tonkinensis radix</em>, has demonstrated a protective effect against acetaminophen (APAP)-induced liver injury (AILI). The underlying molecular mechanism was investigated in this study. Here, an acute liver damage mouse model was generated by APAP (400 mg/kg) and used to identify the protective effect of STRP1 (200 mg/kg) on mouse livers. In vitro cell experiments were used to further verify the related signaling pathways. Initially, in our study, STRP1 treatment reduced APAP-induced liver injury by decreasing aminotransferase activity and cell apoptosis and increasing cell proliferation. Furthermore, STRP1 treatment significantly increased <em>Nrf2</em> expression and alleviated oxidative stress caused by reactive oxygen species in AILI. Based on bioinformatics and experimental studies, miR-140-5p was identified and found to be reduced by STRP1, increasing <em>Nrf2</em> expression. Additionally, <em>Nrf2</em> played an important role in the protective impact of STRP1-suppressed miR-140-5p expression. Generally, these results showed that STRP1-mediated suppression of miR-140-5p expression mitigates AILI by activating the <em>Nrf2</em>-mediated Nrf2-Keap1 pathway. This study revealed that STRP1 might be a potential treatment agent for AILI.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 467-476"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000063/pdfft?md5=6344fc8428deb46231aa1beaad07448e&pid=1-s2.0-S2225411024000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139540029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atractylodes macrocephala Koidz. and Cuscuta chinensis Lam. extract relieves insulin resistance via PI3K/Akt signalling in diabetic Drosophila","authors":"Yinghong Li ,&nbsp;Ye Xu ,&nbsp;Biwei Zhang ,&nbsp;Zhigang Wang ,&nbsp;Leilei Ma ,&nbsp;Longyu Sun ,&nbsp;Xiuping Wang ,&nbsp;Yimin Lin ,&nbsp;Ji-an Li ,&nbsp;Chenxi Wu","doi":"10.1016/j.jtcme.2024.01.010","DOIUrl":"10.1016/j.jtcme.2024.01.010","url":null,"abstract":"<div><h3>Background and aim</h3><p>Type-2 diabetes mellitus (T2DM) is mainly characterized by insulin resistance (IR) induced by hyperglycaemia and insufficient insulin secretion. We employed a diabetic fly model to examine the effect and molecular mechanism of <em>Atractylodes macrocephala</em> Koidz. and <em>Cuscuta chinensis</em> Lam. (AMK–CCL) extract as traditional Chinese medicine in treating IR and T2DM.</p></div><div><h3>Experimental procedure</h3><p>The contents of the active ingredients (rhamnose, xylose, mannose, and hyperoside) in AMK–CCL extract were determined by high-performance liquid chromatography. Wild-type (<em>Cg-</em>GAL4/+) or diabetic (<em>Cg</em> &gt; InR^K1409A) <em>Drosophila</em> flies were divided into the control group or metformin group and AMK–CCL (0.0125, 0.025, 0.05, 0.1 g/ml) groups. Food intake, haemolymph glucose and trehalose, protein, weight, triglycerides (TAG), and glycogen were measured to assess glycolipid metabolism. Phosphatidylinositol-3-kinase (PI3K)/Akt signalling was detected using fluorescent reporters [tGPH, <em>Drosophila</em> forkhead box O (dFoxO)–green fluorescent protein (GFP), <em>Glut1</em>–GFP, 2-NBDG] <em>in vivo</em>. <em>Glut1/</em>3 mRNA levels and Akt phosphorylation levels were detected by quantitative polymerase chain reaction and western blotting, respectively, <em>in vitro</em>.</p></div><div><h3>Results</h3><p>AMK–CCL extract contained 0.038 % rhamnose, 0.017 % xylose, 0.69 % mannose, and 0.039 % hyperoside. AMK–CCL at 0.0125 g/mL significantly suppressed the increase in circulating glucose, and the decrease in body weight, TAG, and glycogen contents of diabetic flies. AMK–CCL improved PI3K activity, Akt phosphorylation, Glut1/3 expression, and glucose uptake in diabetic flies, and also rescued diabetes-induced dFoxO nuclear localisation.</p></div><div><h3>Conclusions</h3><p>These findings indicate that AMK–CCL extract ameliorates IR-induced diabetes via the PI3K/Akt signalling pathway, providing an experimental basis for clinical treatment.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 424-434"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000105/pdfft?md5=6b23edcc8ec860e3dba0c8b648b13f51&pid=1-s2.0-S2225411024000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140527368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective effects of Tradescantia spathacea tea bioactives in Parkinson’s disease: In vivo proof-of-concept","authors":"Lorenna E.S. Lopes ,&nbsp;Sheilla da Silva Barroso ,&nbsp;Joanny K.M. Caldas ,&nbsp;Paulo R. Vasconcelos ,&nbsp;Kirley M. Canuto ,&nbsp;Claudio Dariva ,&nbsp;Klebson S. Santos ,&nbsp;Patricia Severino ,&nbsp;Juliana C. Cardoso ,&nbsp;Eliana B. Souto ,&nbsp;Margarete Z. Gomes","doi":"10.1016/j.jtcme.2024.01.003","DOIUrl":"10.1016/j.jtcme.2024.01.003","url":null,"abstract":"<div><h3>Background and aim</h3><p><em>Tradescantia spathacea</em> (<em>T. spathacea</em>) is a traditional medicinal plant from Central America and its tea, obtained by infusion, has been recognized as a functional food. The aim of this work was to investigate the effects of dry tea containing biocompounds from <em>T. spathacea</em> tea on motor and emotional behavior, as well as tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP) expression in 6-hydroxydopamine (6-OHDA)-lesioned rats.</p></div><div><h3>Experimental procedure</h3><p>Bioactives were identified by Ultra Performance Liquid Chromatography (UPLC) and an <em>in vivo</em> study in male Wistar rats was run as proof of concept of neuroprotective effects of DTTS.</p></div><div><h3>Results and conclusion</h3><p>We found 15 biocompounds that had not been previously reported in <em>T. spathacea</em>: the UPLC-QTOF-MS/MS allowed identification five phenolic acids, one coumarin, two flavonoids, one iridoid, one phenylpropanoid glycoside, and six fatty acid derivatives. The dry tea of <em>T. spathacea</em> (DTTS) presented significant antioxidant activity and high contents of phenolic compounds and flavonoids. Doses of 10, 30, and 100 mg/kg of DTTS were protective against dopaminergic neurodegeneration and exhibited modulatory action on the astrocyte-mediated neuroinflammatory response. Behavioral tests showed that 30 mg/kg of DTTS counteracted motor impairment, while 100 mg/kg produced an anxiolytic effect. The DTTS could be, therefore, a promising strategy for the management of Parkinson's disease.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 435-445"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000038/pdfft?md5=60ab07b9c5f33562dd6de3c77145b80a&pid=1-s2.0-S2225411024000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on ethnomedicinal uses, phytochemistry, toxicology, and pharmacological activities of Dittrichia viscosa (L.) Greuter","authors":"Rania Jerada ,&nbsp;Abdeljalil Er-Rakibi ,&nbsp;Abha Cherkani Hassani ,&nbsp;Hanane Benzeid ,&nbsp;Abdelmoula El Ouardi ,&nbsp;Hicham Harhar ,&nbsp;Bey Hing Goh ,&nbsp;Yoon-Yen Yow ,&nbsp;Hooi-Leng Ser ,&nbsp;Abdelhakim Bouyahya ,&nbsp;Brahim Mojemmi ,&nbsp;Anass Doukkali","doi":"10.1016/j.jtcme.2024.03.012","DOIUrl":"10.1016/j.jtcme.2024.03.012","url":null,"abstract":"<div><p><em>Dittrichia viscosa</em> is a perennial herb that has been used for generations in traditional medicine to address a variety of diseases, including diabetes, hypertension, cancer, microbial disorders, inflammatory conditions, and wound healing. The objective of this review is to provide an overview of existing knowledge on <em>D. viscosa</em> with regards to its botanical description, ethnomedicinal uses, and pharmacological properties. Databases such as Scopus, Wiley-Online, PubMed, Springer, Google Scholar, and ScienceDirect were used to select relevant articles based on their title and abstract.</p><p>The reviewed studies found a strong correlation between <em>D. viscosa</em>'s traditional uses and its observed biological effects. Pharmacological research has shown that the essential oils and extracts from <em>D. viscosa</em> possess a variety of biological activities, such as anti-inflammatory, anticancer, antibacterial, antifungal, analgesic, and antioxidant properties. The chemical compounds found in <em>D. viscosa</em> include sesquiterpenes, monoterpenes, flavonoids, and phenolic acids; some of these compounds, such as tometosin and inuviscolide, have been isolated and displayed promising cytotoxic and anti-inflammatory activity.</p><p>The present review suggests that the pharmacological properties of <em>D. viscosa</em> align well with its ethnomedicinal uses. These findings support the traditional use of <em>D. viscosa</em> in treating various illnesses. Additionally, toxicological examinations of <em>D. viscosa</em> extracts and essential oil have demonstrated the plant's safety, which supports the need for comprehensive pharmacological studies, <em>in vivo</em> studies, and clinical trials to evaluate the best doses for optimal medicinal effects. This work underscores the medicinal value of <em>D. viscosa</em> and its potential in developing new pharmacological agents to address major health challenges like antibiotic resistance and cancers.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 355-380"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000324/pdfft?md5=7ba9811db09e71ac401faaac781fbcf1&pid=1-s2.0-S2225411024000324-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140272571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute toxicity profiling of medicinal herb Ardisia elliptica leaf extract by conventional evaluations and proton nuclear magnetic resonance (NMR) metabolomics","authors":"Pei Lou Wong ,&nbsp;Nur Khaleeda Zulaikha Zolkeflee ,&nbsp;Nurul Shazini Ramli ,&nbsp;Chin Ping Tan ,&nbsp;Azrina Azlan ,&nbsp;Faridah Abas","doi":"10.1016/j.jtcme.2024.02.001","DOIUrl":"10.1016/j.jtcme.2024.02.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of <em>Ardisia elliptica</em>, a traditional medicinal plant used in the treatment of various illnesses.</p></div><div><h3>Experimental procedure</h3><p>Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract, using a combination of conventional investigations and ¹H-NMR-based metabolomics approaches.</p></div><div><h3>Results</h3><p>Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. ¹H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, ¹H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.</p></div><div><h3>Conclusion</h3><p>This study suggested that 2000 mg/kg BW of 70% ethanolic <em>A. elliptica</em> leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 456-466"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000117/pdfft?md5=0cd1d2568e7879636a4f67485bf5bedf&pid=1-s2.0-S2225411024000117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of stimulating single acupoint and combination acupoints on diabetic gastroparesis: A randomised controlled trial study","authors":"Renming Liu ,&nbsp;Min He ,&nbsp;Xuewei Zhao ,&nbsp;Mengmeng Sun ,&nbsp;Jiazhen Cao ,&nbsp;Xinyue Wang ,&nbsp;Xingbang Wang ,&nbsp;Shuming Zhao ,&nbsp;Fuchun Wang ,&nbsp;Tie Li","doi":"10.1016/j.jtcme.2024.01.008","DOIUrl":"10.1016/j.jtcme.2024.01.008","url":null,"abstract":"<div><h3>Background and aim</h3><p>The most effective among the acupoints remains to be determined for treating diabetic gastroparesis (DGP). This study aimed to compare single and combination acupoints for their effectiveness in DGP.</p></div><div><h3>Experimental procedure</h3><p>A prospective, patient-assessor-blinded randomised controlled trial was designed to compare the efficacy of 8-week acupuncture at a single acupoint (Zhongwan, CV-12), combination acupoints (Zhongwan, CV-12 and Zusanli, ST-36), and a sham-acupoint, in 99 adults with DGP. The primary clinical outcome was measured using the Gastroparesis Cardinal Symptom Index (GCSI), while barium meal examination, fasting plasma glucose, the 2-h plasma glucose, short-form health survey (SF-36), and GCSI subscales were performed for evaluating secondary clinical outcomes. These results were analysed by two factorial analysis of variance (ANOVA) test, Chi-Square, Fisher Exact, Kruskal–Wallis tests and Tukey's Honest Significant Difference (HSD) test.</p></div><div><h3>Results</h3><p>After randomization, 97 patients completed the study. GCSI scores of all groups decreased during both post-treatment and the follow-up period, they were statistically significant compared to the baseline period (<em>p</em> &lt; 0.01), but there was no significant difference among the groups (<em>p</em> &gt; 0.05) during the post-treatment period. GCSI scores improved more in the combination acupoints group than in the single acupoint group which was better than the sham group after treatment. During the follow-up period, the same trend was observed.</p></div><div><h3>Conclusions</h3><p>Among patients with DGP, the combination acupoints were more beneficial compared with single and sham acupoints.</p></div><div><h3>Trial registration number</h3><p>NCT02452489.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 446-455"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000087/pdfft?md5=2dd43bff8074cfa32227d692779c7b07&pid=1-s2.0-S2225411024000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139636381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red rice bran aqueous extract ameliorate diabetic status by inhibiting intestinal glucose transport in high fat diet/STZ-induced diabetic rats","authors":"Atcharaporn Ontawong ,&nbsp;Sirinat Pengnet ,&nbsp;Arthid Thim-Uam ,&nbsp;Chutima S. Vaddhanaphuti ,&nbsp;Narongsuk Munkong ,&nbsp;Manussaborn Phatsara ,&nbsp;Kullanat Kuntakhut ,&nbsp;Jakkapong Inchai ,&nbsp;Doungporn Amornlerdpison ,&nbsp;Teerawat Rattanaphot","doi":"10.1016/j.jtcme.2023.12.003","DOIUrl":"10.1016/j.jtcme.2023.12.003","url":null,"abstract":"<div><p>Red rice (<em>Oryza sativa</em> L.) consumption has grown recently, partly due to its potential health benefits in several disease prevention. The impact of red rice bran aqueous extract (RRBE) on intestinal glucose uptake and diabetes mellitus (DM) progression has not been thoroughly investigated. This study aimed to evaluate the effect of RRBE on <em>ex vivo</em> intestinal glucose absorption and its potential as an antihyperglycemic compound using a high-fat diet and streptozotocin (STZ)-induced diabetic rats. High-fat diet/STZ-induced diabetic rats were supplemented with either 1000 mg/kg body weight (BW) of RRBE, 70 mg/kg BW of metformin (Met), or a combination of RRBE and Met for 3 months. Plasma parameters, intestinal glucose transport, morphology, liver and soleus muscle glycogen accumulation were assessed. Treatment with RRBE, metformin, or combination markedly reversed hyperglycemia, hypertriglyceridemia, insulin resistance, and pancreatic morphology changes associated with T2DM. Correspondingly, all supplements effectively downregulated glucose transporters, resulting in a reduction of intestinal glucose transport—additionally, liver and soleus muscle glycogen accumulation was reduced in RRBE + Met treated group. Taken together, RRBE potentially suppressed intestinal glucose transporters' function and expression, reducing diabetic status.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 391-402"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001372/pdfft?md5=663a0e0f9b7bc8979fe2cc809ab8ed5e&pid=1-s2.0-S2225411023001372-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139188804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic effect and metabolic mechanism analysis of Guilingji on idiopathic oligo-asthenoteratozoospermia","authors":"Yuming Feng ,&nbsp;Shanmeizi Zhao ,&nbsp;Rong Ju ,&nbsp;Jianbo Li ,&nbsp;Chengshuang Pan ,&nbsp;Qinglai Tang ,&nbsp;Zhichuan Zou ,&nbsp;Jun Jing ,&nbsp;Yao Xu ,&nbsp;Hualong Ding ,&nbsp;Jinzhao Ma ,&nbsp;Zhou Li ,&nbsp;Ting Tang ,&nbsp;Yong Shao ,&nbsp;Li Chen ,&nbsp;Xuefeng Huang ,&nbsp;Xiaohong Wang ,&nbsp;Bing Yao","doi":"10.1016/j.jtcme.2024.01.001","DOIUrl":"10.1016/j.jtcme.2024.01.001","url":null,"abstract":"<div><h3>Introduction</h3><p>Guilingji, a famous traditional Chinese medicine (TCM) formula, has been used to combat aging and male sexual dysfunction in China for centuries. To date, there has been little evidence-based clinical research on the use of Guilingji to treat idiopathic oligo-asthenoteratozoospermia (OAT), and the therapeutic mechanism from a metabolic perspective needs to be investigated further.</p></div><div><h3>Methods</h3><p>This was a multicenter, double-blind, randomized controlled clinical study of 240 patients with idiopathic OAT recruited from four hospitals between January 2020 and January 2022. Patients were randomly assigned in a 1꞉1 ratio to receive oral Guilingji capsules or placebo for 12 weeks. The total progressive motile sperm count (TPMSC) was considered the primary outcome, and the other sperm parameters, seminal plasma parameters and serum hormones were considered the secondary outcome. A nontargeted metabolomics analysis of serum from OAT patients before and after Guilingji administration was performed by HPLC–MS to identify key metabolites. Furthermore, we used a rat model to show spermatogenesis phenotypes to validate the effect of the key metabolites screened from the patients.</p></div><div><h3>Results</h3><p>At weeks 4, 8 and 12, TPMSC and other sperm parameters were significantly improved in the Guilingji group compared with the placebo group (<em>P</em> &lt; 0.05 for all comparisons). At week 4, superoxide dismutase (SOD) and acrosomal enzyme activity of seminal plasma were significantly elevated in the Guilingji group compared with the placebo group, while reactive oxygen species (ROS) levels were significantly reduced (<em>P</em> &lt; 0.05). Lactate dehydrogenase-X (LDHX) levels appeared to be significantly increased after 12 weeks continuous medication compared with Placebo group (<em>P</em> = 0.032). The metabolomics analysis of serum from OAT patients before and after Guilingji administration showed that the glucose-6-phosphate (G6P) concentration in patients' serum was significantly elevated after Guilingji treatment. Compared to the control, when Kidney-Yang deficiency model rats were treated with Guilingji or its key intermediate metabolite G6P, their sperm concentration and spermatozoic activity were improved similarly, and their structural damage of rat's testicular and epididymal tissues were recovered.</p></div><div><h3>Conclusion</h3><p>This study provided valuable clinical evidence for the utility of Guilingji as a treatment for OAT. These findings thus demonstrate that G6P is involved in the therapeutic mechanism of Guilingji in OAT treatment based on clinical and rat intervention studies.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 4","pages":"Pages 403-413"},"PeriodicalIF":3.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411024000014/pdfft?md5=1beea949ce36b7b4c117aec01a97eabc&pid=1-s2.0-S2225411024000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139391853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}