Journal of Traditional and Complementary Medicine最新文献

{"title":"Unveiling pharmacological targets of Rihimaside C for radiation-induced lung injury: An in silico and experimental integrated approach","authors":"Youyi Liu ,&nbsp;Jingrou Guo ,&nbsp;Chuang Liu ,&nbsp;Xingyi Chen ,&nbsp;Yifei Tang ,&nbsp;Minchen Wu ,&nbsp;Jianfeng Huang","doi":"10.1016/j.jtcme.2024.05.009","DOIUrl":"10.1016/j.jtcme.2024.05.009","url":null,"abstract":"<div><h3>Background and aim</h3><div>Radiation-induced lung injury (RILI) is a common complication during caner radiotherapy, mainly characterized by oxidative stress and inflammation. Rihimaside C, a novel dihydroflavonol compound isolated from <em>Ribes himalense</em>, exhibits significant anti-inflammatory and antioxidant properties. The study aims to investigate the therapeutic efficacy of Rihimaside C in treating RILI, as well as to uncover the potential targets and mechanisms involved.</div></div><div><h3>Experimental procedure</h3><div>Animal experiments were performed to evaluate the pharmacological efficacy of Rihimaside C for RILI. A computer-based strategy was employed to retrieve and screen potential targets for the therapy of Rihimaside C against RILI. STRING, DAVID databases, and Cytoscape software were utilized to construct a protein-protein interaction network and identify hub targets. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted to illuminate the underlying mechanisms. Molecular docking and Cellular Thermal Shift Assay (CETSA) were performed to further validate the hub targets.</div></div><div><h3>Results and conclusion</h3><div>The results of animal experiments showed that Rihimaside C effectively alleviated RILI. Four hub targets (TNF, HSP90AA1, ESR1 and HIF1A) among the 72 possible targets of Rihimaside C involved in the treatment of RILI were finally identified through network pharmacology, which were enriched in MAPK, IL-17, and PI3K/Akt signaling pathways. Molecular docking and CETSA analyses indicated that HSP90AA1 displayed highest binding affinity with Rihimaside C. This study investigated the therapeutic effects of Rihimaside C on RILI and identified potential targets, providing a novel strategy in treating RILI.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 286-295"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of recent artificial intelligence for traditional medicine","authors":"Chengbin Hou ,&nbsp;Yanzhuo Gao ,&nbsp;Xinyu Lin ,&nbsp;Jinchao Wu ,&nbsp;Ning Li ,&nbsp;Hairong Lv ,&nbsp;William Cheng-Chung Chu","doi":"10.1016/j.jtcme.2025.02.009","DOIUrl":"10.1016/j.jtcme.2025.02.009","url":null,"abstract":"<div><div>Traditional Medicine (TM) has played a crucial role in global healthcare due to its long history and holistic approach. Artificial Intelligence (AI) has emerged as a revolutionary technology, offering exceptional capabilities in areas such as data mining, pattern recognition, and decision-making. The integration of Artificial Intelligence for Traditional Medicine (AITM) presents a promising frontier in advancing medicine and healthcare. In this review, we explore AITM from two perspectives: recent AI techniques and TM applications. Specifically, we investigate how Machine Learning, Deep Learning, and Large Language Models are applied to TM, covering applications such as diagnosis (before, during, after) and research (drug research, structured knowledge, data analysis). By leveraging advanced algorithms and models, AI can improve decision-making efficiency, optimize diagnosis accuracy, enhance patient experience, and reduce costs. We anticipate this review can bridge the gap between AI and TM communities. And the goal is to foster collaboration and innovation between both communities, enabling them to exploit the state-of-the-art AI techniques to advance TM diagnosis and research, ultimately contributing to the enhancement of human health.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 215-228"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated serum pharmacochemistry and network pharmacology to reveal the kernel material basis and underlying mechanisms of the fuzi-lizhong pill for ulcerative colitis","authors":"You Huang ,&nbsp;Xia Lin ,&nbsp;Qiuhong Wu ,&nbsp;XunJian Wu ,&nbsp;Shasha Yang ,&nbsp;Yidian Dong ,&nbsp;Chaomei Fu ,&nbsp;Wei Lin ,&nbsp;Zhen Zhang","doi":"10.1016/j.jtcme.2024.06.003","DOIUrl":"10.1016/j.jtcme.2024.06.003","url":null,"abstract":"<div><h3>Background</h3><div>In traditional Chinese medicine, Fuzi-Lizhong pill (FLZP) has been used for millennia as a treatment for the Spleen-Kidney-Yang-deficiency (SKYD) diseases. FLZP has increasingly been employed in the clinic as a therapeutic option for ulcerative colitis with SKYD syndrome (SKYD-UC). In the present study, we revealed the kernel material basis and underlying mechanisms of the FLZP for treating SKYD-UC.</div></div><div><h3>Methods and results</h3><div>The therapeutic effects of FLZP were assessed in SKYD-UC rats. In total, 55 absorbed components of FLZP were identified, thus forming the main material basis for the use of FLZP for treating SKYD-UC. Network pharmacology analyses revealed that the ability of FLZP to exert multi-target synergistic activity was found to be related to both antioxidant and anti-inflammatory activity. More specifically, FLZP was suggested to alleviate SKYD-UC through the regulation of targets associated with inflammation such as interleukin-6 (IL-6), myeloperoxidase (MPO), and tumor necrosis factor-α (TNF-α), while also regulating the mitogen-activated protein kinase (MAPK), TNF, and phosphoinositol-3 kinase-RAC-alpha serine/threonine-protein kinase (PI3K-Akt) pathways. Ultimately, the integration of network analyses, molecular docking studies, and Pearson correlation analyses enabled the identification of 9 core compounds (including linolenic acid, liquirtigenin, 7-hydroxycoumarin, glycyrrhizic acid, 6-shogaol, dehydro-10-gingerdione, caffeic acid, 6-gingerol, liquiritin), which can serve as kernel material basis for FLZP in the treatment of SKYD-UC.</div></div><div><h3>Conclusion</h3><div>Together, these findings offer a valuable foundation for additional research focused on the mechanistic effects and broader clinical application of FLZP as a treatment option for SKYD-UC.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 307-318"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141392959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology approach and experimental verification of earthworm protein in the treatment of diabetes mellitus-induced erectile dysfunction","authors":"Liming Liu ,&nbsp;Yuanfeng Zhang ,&nbsp;Aiping Zhang ,&nbsp;Rui Yan ,&nbsp;Xiaowei Ji ,&nbsp;Jiashu Yang ,&nbsp;Xinping Wang ,&nbsp;Yongze Gao ,&nbsp;Xiping Xing","doi":"10.1016/j.jtcme.2024.06.002","DOIUrl":"10.1016/j.jtcme.2024.06.002","url":null,"abstract":"<div><h3>Background</h3><div>Diabetes mellitus-induced erectile dysfunction (DMED) is a common complication of diabetes mellitus. Earthworm protein (EWP) is an active protein extracted from the Chinese herbal medicine earthworm, which is used in clinical practice for treating DMED.</div></div><div><h3>Objective</h3><div>To investigate the mechanism of action of EWP in improving DMED in rats using network pharmacology and in vivo experimental validation.</div></div><div><h3>Materials and methods</h3><div>Network pharmacology predicts key targets. After identifying the DMED targets of EWP, a protein-protein interaction network was constructed using the STRING platform. The target genes were then enriched using Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes. A “drug-component-disease-target-pathway” network map with Cytoscape 3.9.1 software was constructed. The nuclear factor-kappa B (NF-κB) signaling pathway was selected for further in vivo experimental validation in rats.</div></div><div><h3>Results</h3><div>EWP was mainly involved in the inflammatory response and NF-κB signaling pathway to regulate DMED. In vivo experiments showed that EWP was able to reduce Interleukin-1β, Interleukin-6 and Tumour Necrosis Factor-α levels, significantly inhibit NF-κB, nuclear factor-κB inhibitor protein α and mRNA expression, increase serum testosterone (T), and improve the erectile function of DMED rats.</div></div><div><h3>Conclusion</h3><div>EWP improves erectile function in DMED rats. This mechanism may be related to the inhibition of the NF-κB signaling pathway, reduction of the inflammatory response in testicular tissue, promotion of testicular and penile tissue repair, and increase in serum T levels.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 296-306"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141400215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragalus mongholicus polysaccharides alleviate insulin resistance through modulation of PI3K/AKT, TLR4/NF-kB signaling pathway and microbiota in rats with Type 2 Diabetes Mellitus","authors":"Haisheng Yuan ,&nbsp;Guoquan Xu ,&nbsp;Jingran Liu ,&nbsp;Yan Yan ,&nbsp;Shimin Zhao ,&nbsp;Fujuan Cai ,&nbsp;Xiuling Yu ,&nbsp;Yuzhen Wang ,&nbsp;Minhui Li","doi":"10.1016/j.jtcme.2024.05.007","DOIUrl":"10.1016/j.jtcme.2024.05.007","url":null,"abstract":"<div><h3>Background and aim</h3><div><em>Astragali Radix</em> has been widely used in traditional Chinese medicine to treat diabetes and a variety of other diseases. This study aims to evaluate the alleviating effects and mechanisms of <em>Astragalus mongholicus</em> Polysaccharide (mAPS) against diet combined with streptozotocin (STZ)-induced Type 2 Diabetes Mellitus (T2DM).</div></div><div><h3>Experimental procedure</h3><div>T2DM rats were orally administrated either with 200 mg/kg mAPS or 300 mg/kg Metformin (MET) once daily for four weeks. Body weight and Fasting Blood Glucose (FBG) were detected every 6 days. Serum fasting insulin (FINS) was measured by ELISA and the homeostatic model assessment of insulin resistance (HOMA-IR) was calculated accordingly. Histological change was studied by Hematoxylin and eosin (HE) staining. 16S rDNA sequencing was used to detect the changes in gut microbiota.</div></div><div><h3>Results and conclusion</h3><div>Oral administration of mAPS significantly decreased body weight, FBG, and HOMA-IR in T2DM rats (<em>p</em>＜0.05). Moreover, HE staining showed that mAPS could alleviate histological distortion in the liver and pancreas. Treatment with mAPS elevated the hepatic levels of phosphatidylinositol-3 kinase (PI3K), phospho-protein kinase B (AKT), and glucose transporter type 4 (GLUT4), while reducing phospho-nuclear factor kappa-B (NF-κB), Toll-like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) (<em>p</em>＜0.05). Furthermore, mAPS supplementation could reverse the ratio of <em>Firmicutes</em>/<em>Bacteroidetes</em> (F/B) and reduce the abundance of <em>Clostridia</em> and <em>Proteobacteria</em> (<em>p</em>＜0.05). These results indicate that mAPS have the potential to enhance insulin sensitivity in diabetic rats by modifying gut microbiota and controlling the hepatic glycolipid metabolism and inflammation.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 274-285"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141138041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough","authors":"Leyi Huang ,&nbsp;Yangyang Wang ,&nbsp;Xinyi Yue ,&nbsp;Fangfang Yu ,&nbsp;Tong Wu ,&nbsp;Yimeng Ge ,&nbsp;Chunmiao Wang ,&nbsp;Meihong Tong","doi":"10.1016/j.jtcme.2024.04.006","DOIUrl":"10.1016/j.jtcme.2024.04.006","url":null,"abstract":"<div><h3>Background and aim</h3><div>Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pharmacokinetics and network pharmacology.</div></div><div><h3>Materials and methods</h3><div>PIC rats were measured for cough frequency, organ index, pathological section of lung and inflammatory factors to evaluate the effects of QLOL. Pharmacokinetic analyses were performed using HPLC-QQQ-MS/MS to explore the possible Q-markers of QLOL and the changes of them <em>in vivo</em>. Network pharmacology was used to obtain potential important targets and action mechanisms of treating PIC. The possible hub genes were evaluated using QPCR.</div></div><div><h3>Results</h3><div>The symptoms of cough and lung injury were significantly alleviated and IL-6 and IL-1β were significantly decreased after treatment of QLOL. 6 compounds were considered as possible Q-makers and their pharmacokinetic parameters were analyzed. The compound-target network was constructed to identify 53 important targets. Among them, HSPA8, HSP90AA1, HSP90AB1, YWHAZ, EGFR, ESR1 and EP300 were selected as the core targets because of high degree value and direct connection with inflammation or microbial infection. All 6 compounds had potently binding activity to core targets in molecular docking. The QPCR results showed the up-regulation of core targets expression in QLOL group compared with PIC rats, which validated the effects of QLOL and the accuracy of Q-markers selection.</div></div><div><h3>Conclusion</h3><div>QLOL alleviated PIC symptoms through various targets and mechanisms. The potential Q-markers were baicalein, baicalin, oroxylin A-7-<em>O</em>-glucuronideloside, wogonoside, oroxylin A and forsythoside E.</div></div><div><h3>Taxonomy (classification by evise)</h3><div>the experimental approach.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 250-263"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140797251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Himatanthus bracteatus stem bark ethanolic extract obtained by sequential pressurized liquid extraction: Chromatographic characterization and profiling of cytotoxic, antitumoral and immunopharmacological properties","authors":"Rose N. Pereira-Filho ,&nbsp;Wilson D. Gonçalves-Júnior ,&nbsp;Agenor G. dos Santos-Neto ,&nbsp;John L.S. Cunha ,&nbsp;Oslei P. de Almeida ,&nbsp;Luciana N. Andrade ,&nbsp;Daniela Droppa-Almeida ,&nbsp;Ricardo G. Amaral ,&nbsp;Cláudio Dariva ,&nbsp;Juliana C. Cardoso ,&nbsp;Patricia Severino ,&nbsp;Eliana B. Souto ,&nbsp;Ricardo L.C. de Albuquerque-Júnior","doi":"10.1016/j.jtcme.2024.06.004","DOIUrl":"10.1016/j.jtcme.2024.06.004","url":null,"abstract":"<div><div>This study aims to characterize the cytotoxic, antitumoral and immunopharmacological profile of the ethanolic extract of <em>Himatanthus bracteatus</em> (EEHB) stem bark. Chromatographic analysis revealed the major EEHB composition in dimethyl isoplumerideo acid, 13-deoxyplumerido, isoplumeride, and plumeride. Cytotoxicity was performed on MCF-7 and MCF-10A cell lines using MTT assay. The antitumor activity was assessed using sarcoma 180 tumor cells subcutaneously implanted in mice. After seven days, hematological and biochemical analysis, and pathological evaluation of tumors and visceral organs were carried out. The IC₅₀ value was 28.49 ± 2.05 μg/mL on MCF7 cells, but over 320 μg/mL on MCF-10A cells. Molecular docking was predicted using the caspase 3 molecular target with plumeride and isoplumeride ligands. Both compounds were also analyzed by PreADMET. The tumor growth inhibition was comparable to 5-FU. EEHB reduced the proliferative index (Ki67 immunoexpression) but increased the expression of apoptotic markers in a sarcoma 180 model. The ligands showed interaction with Caspase 3 with a binding energy between −7.2 and −6.6 kcal/mol for isoplumeride and −7.8 to −7.0 kcal/mol for plumeride. Hydrogen interactions were present between the ligands and caspase 3. Both phytochemicals showed low or no permeability in blood-brain barrier and medium permeability in Caco-2 cells and only isoplumeride showed mutagenic potential and carcinogenic. EEHB presented no toxicological effect either on the hematological parameters or average weight and histological features of liver, kidneys, and spleen. Our data suggest that EEHB has antitumor activity in S-180 tumor-bearing mice by blocking cell cycle and increasing apoptosis.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 319-329"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141397265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology-based study to investigate the mechanism of compound houttuynia mixture against influenza virus infection by suppressing TLR7/MyD88 signaling pathway","authors":"Hailin Wei ,&nbsp;Wenlei Wang ,&nbsp;Qin Su ,&nbsp;Zhihui Zheng ,&nbsp;Zihan Chen ,&nbsp;Xinyue Zhang ,&nbsp;Yihan Xu ,&nbsp;Xiaoquan Wang ,&nbsp;Pinghu Zhang","doi":"10.1016/j.jtcme.2024.03.008","DOIUrl":"10.1016/j.jtcme.2024.03.008","url":null,"abstract":"<div><h3>Aim of the study</h3><div>Compound houttuynia mixture (CHM) has been approved to cure respiratory diseases in China. However, the anti-influenza virus effect and underlying mechanism of CHM remains to be confirmed.</div></div><div><h3>Materials and methods</h3><div>The <em>in vitro</em> activity of CHM against H3N2 virus was evaluated using MDCK, A549 and THP-1 cells as an <em>in vitro</em> model. The <em>in vivo</em> protective effect on H3N2 virus infection was investigated. Moreover, serum cytokines were measured with high throughput liquid phase protein chip. The anti-influenza mechanism of CHM was predicted by network pharmacology and further validated with immunoblotting.</div></div><div><h3>Results</h3><div>Our results indicated that CHM has significant inhibitory effect on the replication of influenza A H3N2 virus. Furthermore, CHM could effectively reduce the mortality caused by lethal H3N2 virus infection by prolonging the survival time, reducing lung pathological injury, and suppressing excessive cytokines storm. Network pharmacology revealed that the protective effect of CHM on influenza virus infection was involved in multiple targets and multiple pathways. Mechanistic validation indicated that inhibiting the excessive activation of TLR7/MyD88 signaling pathway may be the critical mechanism of CHM exerting the protective effect against influenza virus infection.</div></div><div><h3>Conclusion</h3><div>Regulating of TLR7/MyD88/NF-κB signaling pathway in multiple target cells might be one of key mechanisms of CHM by inhibiting virus replication and excessive inflammatory. Our findings indicated that CHM might be an effective treatment for influenza virus infection.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 237-249"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140281268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing therapeutic effects of velvet antler using different omics strategies","authors":"Shang-Tse Ho ,&nbsp;Ching-Yun Kuo ,&nbsp;Ming-Ju Chen","doi":"10.1016/j.jtcme.2024.08.002","DOIUrl":"10.1016/j.jtcme.2024.08.002","url":null,"abstract":"<div><div>Velvet antler (VA) has been widely used in traditional Chinese medicine more than thousand years. Several pharmacological properties of VA have been demonstrated, such as anti-osteoporosis, anti-osteoarthritis, anti-aging, and anti-ischemia-hypoxia effects. Recently, many studies applied different omics in VA studies to illustrate biological pathways or processes, identify bioactive compounds, and discover pharmacological properties. This mini-review article summarizes the application of different omics for investigating therapeutic effects of VA. The limitation and future challenges facing the application of multi-omics in VA is also briefly discussed.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 229-236"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing mechanisms of qing fei bao yuan decoction granules in treating COPD based on LC-MS, network pharmacology and in vivo methods","authors":"Amei Tang ,&nbsp;Yang Liu ,&nbsp;Guoqiang Guan ,&nbsp;Tong Hao ,&nbsp;Feng Cao","doi":"10.1016/j.jtcme.2024.04.005","DOIUrl":"10.1016/j.jtcme.2024.04.005","url":null,"abstract":"<div><h3>Background and aim</h3><div>The current therapeutic interventions of chronic obstructive pulmonary disease offer only partial alleviation of symptoms, leaving the majority of patients with persistent and significant clinical manifestations. This investigation seeks to elucidate the underlying pharmacological mechanisms of Qing Fei Bao Yuan Decoction (QFBYD) employing a multidisciplinary approach that includes network pharmacology and molecular docking techniques.</div></div><div><h3>Experimental procedure</h3><div>The QFBYD formulation were subjected to mass spectrometry analysis, while critical compounds and biological targets were subsequently identified through the TCMSP database. Disease- and drug-specific targets were collated from a plethora of databases, including Batman-TCM, Stitch, Swiss Target Prediction and GeneCards. GO and KEGG pathways were analyzed for the collected targets. A PPI network was constructed using STRING database to isolate core targets. Molecular docking was executed using Auto Dock Tools and PyMOL software, and an animal model of COPD was developed for experimental validation.</div></div><div><h3>Results and conclusions</h3><div>Seven salient compounds and five core biological targets were ascertained through our analysis. Additionally, four compounds demonstrated high-affinity binding to the identified targets. Pathways involving bacterial endotoxin response, oxidative stress regulation, and endothelial cell migration were significantly enriched according to the KEGG database. Animal models substantiated that QFBYD ameliorated pathological hallmarks, enhanced respiratory functionality, mitigated overexpression of pro-inflammatory cytokines, augmented the antioxidant defense mechanism, and suppressed the hyperactivity of the five core targets. The efficacy of QFBYD in COPD treatment may be primarily attributed to its role in moderating inflammatory responses and rectifying oxidative imbalances.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 264-273"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140788186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}