Journal of Traditional and Complementary Medicine最新文献

{"title":"Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties","authors":"Nalini Singh ,&nbsp;Aditi Chatterjee ,&nbsp;Wahengbam Kabita Chanu ,&nbsp;Pradeep Mini Vaishalli ,&nbsp;Chingakham Brajakishor Singh ,&nbsp;Viswanathan Arun Nagaraj","doi":"10.1016/j.jtcme.2023.05.004","DOIUrl":"10.1016/j.jtcme.2023.05.004","url":null,"abstract":"<div><h3>Background and aim</h3><p>Malaria is a global health issue causing substantial morbidity and mortality. Screening of various traditionally important medicinal plants is a key source for the discovery of new antimalarials. We evaluated the antimalarial and antioxidant activities, and performed detailed phytochemical analyses of <em>Toona ciliata</em> MJ Roem aqueous methanolic leaf extract (<em>Tc</em>MLE).</p></div><div><h3>Experimental procedures</h3><p><em>In vitro</em> antiplasmodial studies in <em>Plasmodium falciparum</em> (<em>Pf</em>) 3D7 and <em>Pf</em>Cam3.I^R539T strains were performed by [³H]-hypoxanthine uptake assays. <em>In vitro</em> cytotoxicity in HeLa and HEK293T cell lines was evaluated using MTT assays. Hemolysis assay was performed using RBCs. Phytochemical analysis by GC-MS and <em>in vitro</em> antioxidant studies by DPPH and ABTS assays were performed. <em>In vivo</em> antimalarial studies in <em>Pb</em>-infected mice were carried out using Rane's test and Peters' 4-day test.</p></div><div><h3>Results and conclusions</h3><p><em>Tc</em>MLE showed significant <em>in vitro</em> antioxidant activity and had phytochemicals reported for antimalarial activity. <em>In vitro</em> studies showed prominent antiplasmodial activity against <em>Pf</em>3D7 strain (IC₅₀ ∼22 μg/ml) and <em>Pf</em>Cam3. I^R539Tstrain (IC₅₀ value ∼43 μg/ml). <em>In vitro</em> cytotoxicity studies, <em>in vitro</em> hemolytic assays, and <em>in vivo</em> acute toxicity studies further suggested that <em>Tc</em>MLE is nontoxic. <em>In vivo</em> antimalarial studies using Rane's test showed a significant decrease in parasitemia by ∼70% at 1200 mg/kg doses and delayed the mortality of mice by ∼10–14 days. Peters' 4-day test also showed a similar pattern. The present study demonstrated the antimalarial potential of <em>Tc</em>MLE. These findings deliver a platform for further studies to identify the active components of <em>Tc</em>MLE and discover new antimalarials.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 6","pages":"Pages 550-560"},"PeriodicalIF":4.5,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49125325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the mechanism of action of effective monomeric, berberine of Xianglian Pill in inhibiting human colon cancer cells based on fatty acid synthase target","authors":"Shi-ying Li ,&nbsp;Yun Li ,&nbsp;Zhong-hua Wu ,&nbsp;Zhang-jie Zhou ,&nbsp;Cun-ya Li ,&nbsp;Ting-ting Wu ,&nbsp;Shu-juan Fu ,&nbsp;Zhi-ying Wang ,&nbsp;Zhi-xian Zhong ,&nbsp;Yi Zhong","doi":"10.1016/j.jtcme.2023.05.008","DOIUrl":"10.1016/j.jtcme.2023.05.008","url":null,"abstract":"<div><h3>Background and aim</h3><p>Xianglian Wan (XLW) as a classic prescription of traditional Chinese medicine protects digestive function; however, few studies have investigated its anti-colorectal cancer effects. This study verified that the effective monomer berberine of XLW plays an antitumo r role by regulating the acetyl-CoA carboxylase (ACC)/fatty acid synthase (FASN) lipid metabolism-related signaling pathway.</p></div><div><h3>Experimental procedure</h3><p>The connection between XLW and FASN was identified through literature mining, bioinformatics and structural biology. In vivo experiments verified the rationality of the antitumor effect of berberine by regulating the ACC/FASN pathway, and in vitro experiments verified the regulatory relationship between berberine and FASN.</p></div><div><h3>Results and conclusion</h3><p>The most frequent Chinese medicine component in XLW was <em>Coptis chinensis</em>. Berberine, the active ingredient of XLW, has a FASN binding site. FASN expression is higher in tumor tissues than in normal tissues. FASN is related to colorectal adenocarcinoma occurrence and patient survival time. Experiments showed that XLW, berberine and orlistat (FASN inhibitor) can cooperate with palmitic acid (PA) to inhibit tumors in mice. Berberine can downregulate FASN and ACC expression in tumor tissues and inhibit the increase in acetyl-CoA, the intermediate product of exogenous PA intake. The mechanism by which berberine inhibits colon cancer cell proliferation by lowering lipids is related to its downregulation of FASN protein expression. The ACC/FASN signaling pathway is a critical pathway through which berberine, the effective monomer of XLW, plays an antitumor role in colon cancer.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 6","pages":"Pages 538-549"},"PeriodicalIF":4.5,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48379377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM2 - Aims and scope","authors":"","doi":"10.1016/S2225-4110(23)00106-2","DOIUrl":"https://doi.org/10.1016/S2225-4110(23)00106-2","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 6","pages":"Page ii"},"PeriodicalIF":4.5,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71753070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antispasmodic, cardioprotective and blood-pressure lowering properties of Gomphrena perennis L. and its mechanisms of action","authors":"Adriana Milena Bonilla Bonilla ,&nbsp;Tatiana C. Gavilánez Buñay ,&nbsp;Matías Bayley ,&nbsp;Germán A. Colareda ,&nbsp;Soledad I. Matera ,&nbsp;María Luján Flores ,&nbsp;Osvaldo León Córdoba ,&nbsp;Julián J. Prieto ,&nbsp;María Esperanza Ruiz ,&nbsp;Alicia E. Consolini ,&nbsp;María Inés Ragone","doi":"10.1016/j.jtcme.2023.10.005","DOIUrl":"10.1016/j.jtcme.2023.10.005","url":null,"abstract":"<div><h3>Background</h3><p><em>Gomphrena perennis</em> L. is a native plant of South America whose pharmacological properties have not been studied yet.</p></div><div><h3>Aim</h3><p>To evaluate the cardiovascular and intestinal pharmacological effects of <em>Gomphrena perennis</em> L. leaves tincture (GphT) and the mechanisms involved.</p></div><div><h3>Experimental procedure</h3><p>The chromatographic profile of GphT was done. Its <em>ex vivo</em> effects were evaluated by contractile concentration-response curves (CRCs) obtained from the agonist carbachol or calcium found in isolated rat small intestine, as well as in the relaxant CRCs. Cardiac effects were evaluated on isolated rat hearts exposed to ischemia/reperfusion (I/R). Experiments <em>in vivo</em> were performed to evaluate the diuretic activity in conscious rats and the hypotensive effect in anaesthetised rats.</p></div><div><h3>Results</h3><p>Fifteen flavonoids were identified in GphT by HPLC-UV, including diosmin. GphT induced a non-competitive inhibition in both carbachol and calcium CRCs on rat small intestine. The first was not affected by indomethacin. Moreover, GphT, unlike diosmin, relaxed the contracture produced by a high-potassium solution in a dose-dependently way. Neither propranolol nor <span>l</span>-NAME changed it. GphT did not show diuretic activity but induced hypotension insensitive to <span>l</span>-NAME. While GphT perfusion of isolated hearts increased injury consequent to I/R, oral administration was cardioprotective and reversed by <span>l</span>-NAME. However, diosmin did not improve the post-ischemic recovery.</p></div><div><h3>Conclusions</h3><p>This study supports the use of <em>Gomphrena perennis</em> L. tincture as an antispasmodic and hypotensive agent. Moreover, it has been demonstrated to be preventive of post-ischemic cardiac dysfunction. However, diosmin would not be responsible for these effects.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 182-190"},"PeriodicalIF":4.5,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001128/pdfft?md5=a830411edcdb54fde8447f7173d4b611&pid=1-s2.0-S2225411023001128-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136128228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qingguang'an-induced autophagy in TFs inhibits scar formation: A follow-up in vivo mechanistic investigation","authors":"Rong Hu ,&nbsp;Xian-jing Wang ,&nbsp;Shu Chen ,&nbsp;Yun Huang ,&nbsp;Juan Yu","doi":"10.1016/j.jtcme.2023.10.002","DOIUrl":"10.1016/j.jtcme.2023.10.002","url":null,"abstract":"<div><h3>Purpose</h3><p>To investigate the mechanism by which Qingguang'an inhibits scar formation in rabbits administered glaucoma filtering surgery (GFS).</p></div><div><h3>Methods</h3><p>Combined trabeculectomy was performed in 100 rabbits diagnosed with glaucoma, which were assigned to five groups, including the no surgery, surgery only, mitomycin C (MMC; positive control), Qingguang'an (experimental) and PBS (negative control) groups. The animals were followed up at postoperative days 1–28. Ultrastructure was observed under a transmission electron microscope (TEM). Real-Time Polymerase Chain Reaction (RT-PCR), Western blot, Hematoxylin and Eosin (H&amp;E) staining, Masson's trichrome staining and Immuno-histochemistry (IHC) were performed to assess the harvested blocks.</p></div><div><h3>Results</h3><p>In the Qingguang'an group, intraocular pressure (IOP) on postoperative D28 was significantly lower than values in the no surgery, surgery only and PBS groups (P &lt; 0.05). Its blebs kept better filtering function and less complications in follow-up, which be detected to have less fibroblasts and collagen deposition histologically. Compared with the PBS group, ATG5, Beclin1 and LC3-II mRNA levels were significantly increased while P62 was downregulated in the Qingguang'an group (P &lt; 0.05). Correspondingly, ATG5 and Beclin1 protein amounts in the Qingguang'an group were increased while P62 was downregulated. The LC3-II/Ⅰ ratio tended to rise to the process of autophagy. Abundant autophagosomes were captured under TEM in this condition.</p></div><div><h3>Conclusions</h3><p>Qingguang'an granules can inhibit scar formation in rabbits after GFS and restrain IOP increase by inducing autophagy in TFs.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 173-181"},"PeriodicalIF":4.5,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001098/pdfft?md5=f5e871d8434c68ae51199f1336e86275&pid=1-s2.0-S2225411023001098-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135852211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis’ [J. Trad, Complementary Med 10 (6) (November 2020) 529–543]","authors":"Amrita Chaudhary ,&nbsp;Swarnendu Bag ,&nbsp;Provas Banerjee ,&nbsp;Jyotirmoy Chatterjee","doi":"10.1016/j.jtcme.2023.10.001","DOIUrl":"10.1016/j.jtcme.2023.10.001","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 350-352"},"PeriodicalIF":4.5,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001025/pdfft?md5=56c43bfbbf3364f2e0a877b67166b24d&pid=1-s2.0-S2225411023001025-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134934909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dachengqi decoction ameliorates sepsis-induced liver injury by inhibiting the TGF-β1/Smad3 pathways","authors":"Guangtao Pan ,&nbsp;Yanran Wu ,&nbsp;Yuhan Liu ,&nbsp;Fangyuan Zhou ,&nbsp;Sen Li ,&nbsp;Shenglan Yang","doi":"10.1016/j.jtcme.2023.09.001","DOIUrl":"10.1016/j.jtcme.2023.09.001","url":null,"abstract":"<div><h3>Background</h3><p>Sepsis-induced acute liver injury (ALI) is a major contributor to mortality in septic patients. Exploring the pathogenesis and developing effective treatment strategies for sepsis-induced ALI is critical for improving patient outcomes. Dachengqi decoction (DCQD), which is a classic Chinese herbal medicine, has been shown to possess potent anti-inflammatory properties. However, the protective effects and underlying mechanisms of DCQD against sepsis-induced ALI remain unclear. This study aimed to investigate the protective effect of DCQD on sepsis-induced ALI and elucidate the involvement of the TGF-1β/Smad3 pathways.</p></div><div><h3>Methods</h3><p>A septic mouse model was established using caecal ligation and puncture (CLP) to evaluate the protective effect of DCQD on sepsis-induced ALI in vivo. An in vitro cellular inflammation model was established using LPS-stimulated LO2 cells to further investigate the underlying mechanism.</p></div><div><h3>Results</h3><p>DCQD (2.5, 5.0, and 10.0 g/kg body weight) was administered twice daily for 2 days and exerted a dose-dependent protective effect against sepsis-induced ALI. DCQD treatment significantly inhibited inappropriate inflammatory responses and oxidative stress in liver tissue. Moreover, DCQD maintained liver homeostasis by inhibiting hepatocyte apoptosis and improving sepsis-induced liver damage. In vivo and in vitro studies indicated that the TGF-β1/Smad3 signalling pathway played an important role in sepsis-induced ALI, and DCQD treatment significantly inhibited the activation of this pathway.</p></div><div><h3>Conclusions</h3><p>DCQD can effectively suppress excessive inflammatory responses and oxidative stress, leading to a substantial reduction in hepatocyte apoptosis in sepsis-induced ALI.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 256-265"},"PeriodicalIF":4.5,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000986/pdfft?md5=def899755182ac87462074234c9986d8&pid=1-s2.0-S2225411023000986-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135298176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wound healing potential of Acacia catechu in streptozotocin-induced diabetic mice using in vivo and in silico approach","authors":"Vinayak P. Nakhate ,&nbsp;Natasha S. Akojwar ,&nbsp;Saurabh K. Sinha ,&nbsp;Amarsinh D. Lomte ,&nbsp;Mahaveer Dhobi ,&nbsp;Prakash R. Itankar ,&nbsp;Satyendra K. Prasad","doi":"10.1016/j.jtcme.2023.05.001","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.05.001","url":null,"abstract":"<div><h3>Background and aim</h3><p><em>Acacia catechu</em> Wild<strong>.</strong> (Fabaceae) barks are traditionally used in the treatment of diabetes and wounds. Therefore, the objective of the present study was to evaluate the wound healing potential of the alcoholic extract of <em>A. catechu</em> (EAC) in streptozotocin-induced diabetic mice.</p></div><div><h3>Experimental procedures</h3><p>EAC was first subjected to phytochemical estimations and standardization using (−) epicatechin as marker with the help of HPLC. Diabetes was induced in mice using streptozotocin and the wound healing potential of EAC was evaluated using excision and incision wound models on topical and oral treatment. Various biochemical parameters, <em>in vivo</em> antioxidants, cytokine profiling, VEGF, and histopathological examination were also performed. Further, molecular docking studies were performed using ligand (−) epicatechin on human inducible nitric oxide synthase.</p></div><div><h3>Results and conclusion</h3><p>Phytochemically, EAC showed the presence of tannins, flavonoids, phenolic compounds, and saponins, while the content of (−) epicatechin was reported to be 7.81% w/w. The maximum healing of wounds (91.84 ± 1.10%) was observed in mice treated with a combination of both topical (10% gel) and oral (extract at 200 mg/kg) followed by topically and orally treated groups respectively after 14 days of treatment. These groups also showed significant restoration of altered biochemical parameters, antioxidant enzymes and cytokines. The molecular docking studies confirmed the role of (−) epicatechin in stabilizing the human inducible nitric oxide synthase with inhibitor showing binding energy of −8.31 kcal/mol. The present study confirmed the role of (−) epicatechin as a major marker in diabetic wound healing potential of <em>A. catechu.</em></p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 5","pages":"Pages 489-499"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49754878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational studies on searching potential phytochemicals against DNA polymerase activity of the monkeypox virus","authors":"Seshu Vardhan,&nbsp;Suban K. Sahoo","doi":"10.1016/j.jtcme.2023.04.002","DOIUrl":"10.1016/j.jtcme.2023.04.002","url":null,"abstract":"<div><h3>Objectives</h3><p>The outbreak of monkeypox virus (MPXV) is an emerging epidemic of medical concern with 65353 confirmed cases of infection and a fatality of 115 worldwide. Since May 2022, MPXV has been rapidly disseminating across the globe through various modes of transmission, including direct contact, respiratory droplets, and consensual sex. Because of the limited medical countermeasures available to treat MPXV, the present study aimed to identify potential phytochemicals (limonoids, triterpenoids, and polyphenols) as antagonists to target the DNA polymerase protein of MPXV with the ultimate goal to inhibit the viral DNA replication mechanism and immune-mediated responses.</p></div><div><h3>Methods</h3><p>The protein-DNA and protein-ligand molecular docking were performed with the help of computational programs AutoDock Vina, iGEMDOCK and HDOCK server. The BIOVIA Discovery studio and ChimeraX were used to evaluate the protein-ligand interactions. The GROMACS 2021 was used for the molecular dynamics simulations. The ADME and toxicity properties were computed by using online servers SwissADME and pKCSM.</p></div><div><h3>Results</h3><p>Molecular docking of 609 phytochemicals and molecular dynamics simulations of lead phytochemicals glycyrrhizinic acid and apigenin-7-O-glucuronide generated useful data that supported the ability of phytochemicals to obstruct the DNA polymerase activity of the monkeypox virus.</p></div><div><h3>Conclusions</h3><p>The computational results supported that appropriate phytochemicals can be used to formulate an adjuvant therapy for the monkeypox virus.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 5","pages":"Pages 465-478"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5d/7e/main.PMC10165885.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10574206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effects and mechanisms of Guizhigancao Decoction on heart failure using an integrated approach based on experimental support and network pharmacology strategy","authors":"Jianhua Qu ,&nbsp;Jiao Wang ,&nbsp;Biao Zheng ,&nbsp;Xiaoxiao Jiang ,&nbsp;Jikui Liu ,&nbsp;Jing Chen","doi":"10.1016/j.jtcme.2023.03.010","DOIUrl":"10.1016/j.jtcme.2023.03.010","url":null,"abstract":"<div><h3>Background and aim</h3><p>HF (Heart Failure) is the leading cause of mortality and is a significant clinical problem affecting millions of patients worldwide. To date, the mechanisms of HF remain largely elusive. The effective treatments contributing to HF remain incompletely understood. Therefore, the development of an effective strategy for HF is urgently needed.</p></div><div><h3>Experimental procedure</h3><p>In the present study, we devoted to investigating the effective treatments and sought to systematically decipher the related molecular mechanisms of Guizhigancao Decoction (GZGCD, Cinnamomum cassia Presl and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle) for treating HF. We examined the therapeutic effect of GZGCD on HF <em>in vivo</em>. An integrative approach combining biomarker examination, echocardiography, myocardial fibrosis and cardiac apoptosis condition using Masson and TUNEL staining was performed to assess the efficacy of GZGCD against HF. Subsequently, comprehensive network pharmacology analyses were performed to explore the mechanisms involved in GZGCD therapeutic effects on HF.</p></div><div><h3>Results and conclusions</h3><p>The results showed that GZGCD could reverse cardiac function in rats with HF by reducing NT-proBNP, increasing EF, decreasing LVESV, LVEDV, LVIDs, LVIDd, increasing running time, and ameliorate myocardial collagen fiber hyperplasia and cardiomyocyte apoptosis. We showed that GZGCD might contribute to HF treatment via oxidative related pathways through bioinformatics. Eventually, promising compound quercetin in GZGCD for HF therapeutics was proposed in database-based analysis. Collectively, our findings indicate that GZGCD has a treatment effect on HF. We proposed that GZGCD might contribute HF treatment via oxidative response-related pathways.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 5","pages":"Pages 454-464"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/b5/main.PMC10491989.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10276558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}