Journal of Traditional and Complementary Medicine最新文献

{"title":"Antihyperglycemic effect of extra virgin sacha inchi oil in type 2 diabetic rats: Mechanisms involved in pancreatic β-cell function and apoptosis","authors":"Navinee Wongmanee ,&nbsp;Worarat Rojanaverawong ,&nbsp;Tantip Boonsong ,&nbsp;Wanthanee Hanchang","doi":"10.1016/j.jtcme.2023.08.005","DOIUrl":"10.1016/j.jtcme.2023.08.005","url":null,"abstract":"<div><h3>Background and aim</h3><p>The purpose of the study was to investigate the anti-hyperglycemic effect of extra virgin sacha inchi oil (EVSIO) and its possible mechanisms and actions against pancreatic β-cell death and dysfunction in type 2 diabetic (T2D) rats.</p></div><div><h3>Experimental procedure</h3><p>T2D rats were induced with a high-fat diet and low-dose of streptozotocin. The rats were then treated for 5 weeks with EVSIO (0.5, 1, and 2 ml/kg), or pioglitazone. Biochemical and histopathological studies, oxidative and inflammatory markers, and expression of apoptotic-related proteins were then evaluated.</p></div><div><h3>Results</h3><p>EVSIO treatment exhibited a dose-dependent reduction of fasting blood glucose, area under the curve of glucose, total cholesterol, and triglyceride levels in the diabetic rats, while improved pancreatic β-function was demonstrated by increasing pancreatic and serum insulin levels. EVSIO treatment effectively lowered atrophic pancreatic islets and reduced the level of serum and pancreatic MDA in the diabetic rats. In addition to serum and pancreatic GPx activities in the diabetic rats, EVSIO also augmented serum SOD. Increased levels of NF-κB, TNF-α and IL-6 present in the diabetic rats were greatly reduced by EVSIO treatment. Furthermore, EVSIO revealed an anti-apoptotic effect on the diabetic rat pancreas by upregulating Bcl-2, and downregulating Bax and cleaved caspase-3 protein expression.</p></div><div><h3>Conclusion</h3><p>The overall study results demonstrated the potential anti-hyperglycemic effect of EVSIO in the diabetic rats. The beneficial effects of EVSIO may be attributed to its ability to improve pancreatic β-cell function and ameliorate β-cell apoptosis by inhibiting oxidative stress and inflammatory cytokines.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000871/pdfft?md5=455785f356ab96d56a528dab2dcf6da9&pid=1-s2.0-S2225411023000871-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48359460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatic transcriptome discloses the potential targets of Xuefu Zhuyu Decoction ameliorating non-alcoholic fatty liver disease induced by high-fat diet","authors":"Hao Tang ,&nbsp;Jun Wang ,&nbsp;Yi Fang ,&nbsp;Yixiao Yin ,&nbsp;Wei Liu ,&nbsp;Yiyang Hu ,&nbsp;Jinghua Peng","doi":"10.1016/j.jtcme.2023.07.008","DOIUrl":"10.1016/j.jtcme.2023.07.008","url":null,"abstract":"<div><h3>Background and Aim</h3><p>Xuefu Zhuyu decoction (XZD), a traditional Chinese medicinal formula, was firstly recorded in the Qing dynasty of ancient China and previously demonstrated to ameliorate hepatic steatosis. In the present study, the effects of XZD on non-alcoholic fatty liver disease (NAFLD) induced by high-fat diet (HFD) were evaluated in mice and the hepatic transcriptome was detected to disclose the potential mechanisms of XZD.</p></div><div><h3>Experimental procedure</h3><p>The effects of XZD (low- and high-dosage) on NAFLD induced by HFD for 16 weeks were evaluated. Obeticholic acid was used as control drug. Body weight, food intake and index of homeostatic model assessment for insulin resistance (HOMA-IR) were analyzed. Hepatic histology were observed in haematoxylin and eosin stained sections and quantified with NAFLD activity score (NAS). Lipid in hepatocytes was visualized by Oil red staining. Alanine aminotransferase (ALT) and hepatic triglyceride (TG) was measured. The hepatic transcriptom was detected with RNA-sequencing and validated with real-time polymerase chain reaction, western-blotting and hepatic quantitative metabolomics.</p></div><div><h3>Results</h3><p>XZD ameliorated hepatic histology of NAFLD mice, accompanied with decreasing fasting insulin, HOMA-IR, NAS, ALT and hepatic TG. The hepatic transcriptom of NAFLD was significantly reversed by XZD treatment, especially the genes enriched in the pathways of arachidonic acid metabolism, fatty acid degradation, cytokine-cytokine receptor interaction and extracellular matrix (ECM) -receptor interaction. The hepatic quantitative metabolomics analysis confirmed fatty acid degradation as the key targeting pathway of XZD.</p></div><div><h3>Conclusions</h3><p>XZD ameliorated NAFLD induced by HFD, which probably correlated closely to the pathways of fatty acid degradation.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023000834/pdfft?md5=d6577a90de5ce496d196e49aa8df4957&pid=1-s2.0-S2225411023000834-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43782025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antihyperglycemic and anti-type 2 diabetic activity of marine hydroquinone isolated from brown algae (Dictyopteris polypodioides)","authors":"Thi Phuong Thao Truong,&nbsp;Thanh Men Tran,&nbsp;Thi Xuan Trang Dai,&nbsp;Chi Linh Tran","doi":"10.1016/j.jtcme.2023.03.007","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.03.007","url":null,"abstract":"<div><h3>Background and aims</h3><p>Brown algae (<em>Dictyopteris polypodioides</em>) extract (DP) presented high inhibitory potential against α-amylase. The present study aims to isolate, purify and evaluate the antihyperglycemic and anti-type 2 diabetic activities of marine hydroquinone from DP.</p></div><div><h3>Methods</h3><p>Marine hydroquinones were isolated using silica gel, HPLC, and NMR spectroscopy was used to identify compound 1 and compound 2 as zonarol and isozonarol, respectively. The anti-hyperglycemic and anti-type 2 diabetic activities of zonarol were investigated by <em>in vitro</em> assay (α-amylase, α-glucosidase), Lineweaver–Burk plot and Type 2 diabetes mellitus model (T2DM) mice induced by streptozotocin (STZ).</p></div><div><h3>Result</h3><p>Zonarol had the highest content and the strongest inhibitory activity against α-glucosidase (IC₅₀ value of 6.03 mg L⁻¹) and α-amylase (IC₅₀ value of 19.29 mg L⁻¹) in a competitive inhibition and mix-type manner, respectively. The maltose and starch loading tests revealed that zonarol significantly reduced postprandial glycemia after 30 min loading (9.12 and 8.12 mg/dL, respectively), compared to normal (11.37 and 12.37 mg/dL, respectively). Zonarol exhibited pancreatic islet cell rejuvenation, as evidenced by increased pancreatic islet mass, and hence helps in the restoration of insulin levels and therefore improves the glucose metabolism in STZ-induced diabetic mice. Zonarol treatment in T2DM elevated abundant levels of main SCFAs (propionate, butyrate, and valeric acid), which are closely related to glucose metabolism homeostasis.</p></div><div><h3>Conclusion</h3><p>Our finding indicates that zonarol could be used as a food supplement to treat hyperglycemia and diabetes.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49756200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM1 - Title Page","authors":"","doi":"10.1016/S2225-4110(23)00068-8","DOIUrl":"https://doi.org/10.1016/S2225-4110(23)00068-8","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49754521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM2- Aims & Scope","authors":"","doi":"10.1016/S2225-4110(23)00069-X","DOIUrl":"https://doi.org/10.1016/S2225-4110(23)00069-X","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49760205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypericum sampsonii exhibits anti-inflammatory activity in a lipopolysaccharide-induced sepsis mouse model","authors":"Yin-Chieh Hsu ,&nbsp;Shih-Ming Ou ,&nbsp;Kai-Ru Zhuang ,&nbsp;Ai-Ling Kuo ,&nbsp;Wan-Jhen Li ,&nbsp;Chun-Yi Huang ,&nbsp;Chao-Hsiung Lin ,&nbsp;Jih-Jung Chen ,&nbsp;Shu-Ling Fu","doi":"10.1016/j.jtcme.2023.03.002","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.03.002","url":null,"abstract":"<div><h3>Background and aim</h3><p>Sepsis causes an uncontrolled systemic response characterized by excessive inflammation and immune suppression, leading to multiple organ failure and death. An effective therapeutic strategy for sepsis-related syndromes is urgently needed. <em>Hypericum sampsonii</em> Hance (HS) is a folk herbal plant used to treat arthritis and dermatitis, but the anti-inflammatory properties of HS and its related compounds have rarely been investigated. In this study, we aimed to explore the anti-inflammatory effects of HS.</p></div><div><h3>Experimental procedure</h3><p>Models of bacterial lipopolysaccharide (LPS)-induced activated macrophages and endotoxemia mice were used, in which the TLR4/NF-κB signaling pathway is upregulated to trigger inflammatory responses. The HS extract (HSE) was delivered into LPS-induced endotoxemia mice via oral administration. Three compounds were purified using column chromatography and preparative thin layer chromatography and were validated by physical and spectroscopic data.</p></div><div><h3>Results</h3><p>HSE suppressed NF-κB activation and proinflammatory molecules (TNF-α, IL-6, iNOS) in LPS-activated RAW 264.7 macrophages. Furthermore, oral administration of HSE (200 mg/kg) to LPS-treated mice improved the survival rate, restored body temperature, decreased TNF-α and IL-6 in serum, and reduced IL-6 expression in bronchoalveolar lavage fluid (BALF). In lung tissues, HSE reduced LPS-induced leukocyte infiltration and the expression of proinflammatory molecules (TNF-α, IL-6, iNOS, CCL4 and CCL5). Three pure compounds isolated from HSE, including 2,4,6-trihydroxybenzophenone-4-O-geranyl ether, 1-hydroxy-7 methoxyxanthone and euxanthone, were demonstrated to exhibit anti-inflammatory activities in LPS-stimulated RAW 264.7 macrophages.</p></div><div><h3>Conclusion</h3><p>The present study demonstrated the anti-inflammatory effects of HS <em>in vitro</em> and <em>in vivo</em>. Further clinical studies of HS in human sepsis are warranted.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49766324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acacia nilotica stem bark extract ameliorates obesity, hyperlipidemia, and insulin resistance in a rat model of high fat diet-induced obesity","authors":"Samar S. Khalaf ,&nbsp;Ola A. Shalaby ,&nbsp;Ahmed R. Hassan ,&nbsp;Mohamed K. El-Kherbetawy ,&nbsp;Eman T. Mehanna","doi":"10.1016/j.jtcme.2023.03.005","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.03.005","url":null,"abstract":"<div><h3>Background and aim</h3><p><em>Acacia nilotica</em> (<em>A. nilotica</em>) is an imperative plant with many medicinal uses. The current study aimed to investigate the protective effects of the stem bark of <em>A. nilotica</em> and its fractions in a high fat diet (HFD) rat model.</p></div><div><h3>Experimental procedure</h3><p>Seventy-two male albino rats were randomly divided into 9 groups, 8 rats per each. Group 1 was the normal control and received standard balanced diet. All the remaining groups were fed HFD for 8 weeks to induce obesity. Group 2 served as the HFD control group, group 3 received orlistat (5 mg/kg/day), groups 4 and 5 received total extract of <em>A. nilotica</em> stem bark (250 and 500 mg/kg). Groups 6 and 7 received <em>A. nilotica</em> ethyl acetate fraction (250 and 500 mg/kg), while groups 8 and 9 received butanol fraction (250 and 500 mg/kg).</p></div><div><h3>Results and conclusion</h3><p>Both doses of the ethyl acetate fraction of the stem bark of <em>A. nilotica</em> significantly decreased the body weight, blood glucose, lipid profile and improved insulin sensitivity. Levels of MDA, leptin and inflammatory cytokines were significantly decreased by the ethyl acetate fraction while adiponectin and HDL-C were significantly increased relative to the HFD control group. Both doses of the ethyl acetate fraction significantly abolished HDF induced oxidative stress and normalized the values of antioxidant enzymes. Furthermore, metabolic profiling of the ethyl acetate fraction was performed by UHPLC/Q-TOF-MS. In conclusion, the ethyl acetate fraction of <em>A. nilotica</em> stem bark possessed antioxidant, anti-inflammatory and insulin sensitizing properties in HFD rat model.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49760182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetes mellitus: Preventive and curative therapies with aqueous extract of Rytigynia senegalensis Blume (Rubiaceae) in Wistar rats","authors":"Barthelemy Maidadi ,&nbsp;Fidèle Ntchapda ,&nbsp;David Miaffo ,&nbsp;Abba Talba Mahamad","doi":"10.1016/j.jtcme.2023.03.001","DOIUrl":"https://doi.org/10.1016/j.jtcme.2023.03.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>African traditional healers use <em>Rytigynia senegalensis</em> Blume to treat diseases such as diabetes mellitus, malaria, dysentery, constipation, and hemorrhoids. This study aimed to assess the hypoglycemic, lipid-lowering, and antioxidant properties of <em>R. senegalensis</em> extract (AERS) in type 1 diabetic (T1D) and insulin-resistant (T2D) rats.</p></div><div><h3>Experimental procedure</h3><p>The induction of T1D was made by intraperitoneal administration of streptozotocin (55 mg/kg b.w). As for T2D, it was induced for 10 days by daily subcutaneous administration of dexamethasone (1 mg/kg b.w). Diabetic animals were divided and treated with AERS (50, 100, and 200 mg/kg b.w) for 28 and 10 days for T1D and T2D, respectively. Glycaemia, food and water consumption, relative body weight, insulinemia, lipid profile, and oxidative stress parameters were evaluated. Histological sections were made on the pancreas of T1D rats.</p></div><div><h3>Results and conclusion</h3><p>AERS (100 and/or 200 mg/kg) prevented (p &lt; 0.05 to p &lt; 0.001) weight loss, polyphagia, and polysipsia in diabetic rats. AERS significantly lowered (p &lt; 0.05 to p &lt; 0.001) insulinemia, hyperglycemia, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c) total cholesterol (TC),and malondialdehyde (MDA). In contrast, a significant increase (p &lt; 0.05 to p &lt; 0.001) in high-density lipoprotein cholesterol (HDL-c) levels, reduced glutathione levels, and superoxide dismutase (SOD) and catalase (CAT) activities were observed with all doses of AERS. Histopathological analysis showed an increase in the number and size of islets of Langerhans in the pancreas of T1D rats receiving AERS. AERS has an important antidiabetic, antidyslipidemic, and antioxidant potential.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49766322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM2- Aims & Scope","authors":"","doi":"10.1016/S2225-4110(23)00049-4","DOIUrl":"https://doi.org/10.1016/S2225-4110(23)00049-4","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49754518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vasorelaxing effect of Garcinia cowa leaf extract in rat thoracic aorta and its underlying mechanisms","authors":"Somruedee Yorsin ,&nbsp;Somchai Sriwiriyajan ,&nbsp;Watchara Chongsa","doi":"10.1016/j.jtcme.2022.12.001","DOIUrl":"https://doi.org/10.1016/j.jtcme.2022.12.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>The leaves of <em>Garcinia cowa (G. cowa)</em> are used in Thai traditional medicine to improve blood circulation. However, there is no scientific evidence to confirm this therapeutic claim. Here, we investigated the vasorelaxing effect and its underlying mechanisms of an aqueous extract of <em>G. cowa</em> leaves in rat thoracic aortic rings.</p></div><div><h3>Materials and methods</h3><p>Dried leaves of <em>G. cowa</em> were extracted with water, followed by phytochemical analysis. Vascular reactivity experiments were performed in isolated rat thoracic aortic rings using an organ bath system. The results were recorded using the data acquisition system Power Lab.</p></div><div><h3>Results</h3><p>Phytochemical analysis showed that the leaves of <em>G. cowa</em> are rich in polyphenols and flavonoids, especially kaempferol, vitexin, and isovitexin. The <em>G. cowa</em> leaf extract caused a concentration-dependent relaxation of aortic rings. This effect was attenuated by denudation of the endothelium, or by pre-treatment of the aortic rings with <span>l</span>-NAME, ODQ, indomethacin, or glibenclamide, but not with TEA.</p></div><div><h3>Conclusion</h3><p>This study indicates that <em>G. cowa</em> leaf extract induces vasorelaxation through both endothelium-dependent and endothelium-independent manners. Its mechanism of action mainly involves the production of nitric oxide and prostanoids, as well as opening ATP-sensitive K⁺ channels. The vasorelaxing effect of <em>G. cowa</em> leaf extract is probable promoted by the action of flavonoids.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49754522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}