Journal of Traditional and Complementary Medicine最新文献

{"title":"Preparation and evaluation of wound healing activity of phytosomes of crocetin from Nyctanthes arbor-tristis in rats","authors":"Manasi Varadkar,&nbsp;Chhaya Gadgoli","doi":"10.1016/j.jtcme.2021.10.002","DOIUrl":"https://doi.org/10.1016/j.jtcme.2021.10.002","url":null,"abstract":"<div><h3>Background and aim</h3><p>Orange colored tubular calyx of the flowers of <em>Nyctanthes arbor-tristis</em> contains an apocarotenoid crocin as a major ingredient, which is originally detected to be major colouring principle of saffron stigma. Saffron stigma exhibits good wound healing activity owing to the presence of crocin. The present study is aimed at isolation of crocetin, from tubular calyx of N. arbortristis and improve stability through entrapping in vesicles followed by evaluation of wound healing activity of the topical formulation thereof.</p></div><div><h3>Experimental procedure</h3><p>Crocetin was isolated by treating ethanolic extract of tubular calyx of <em>N. arbor-tristis</em> with sodium bicarbonate followed by regeneration of crocetin using hydrochloric acid. The phytosomes were prepared by lipid film hydration technique. The gel containing phytosomes equivalent to crocetin 1% w/w, was then evaluated for wound healing activity through applications on incision and excision wounds inflicted in Wister albino rats.</p></div><div><h3>Results</h3><p>Stability of crocetin was found to be increased due to entrapment into phytosomes. The studies revealed that both types of wounds upon treatment with gel containing crocetin phytosomes, indicated good wound healing potential, as the epithelization period was significantly (P &lt; 0.001)decreased as compared to the control group from 26 to 9 days, in excision wound model and significant (P &lt; 0.001)increase in breaking strength of repaired skin, as compared to control from 328.8 to 857.0 gm in incision wound model.</p></div><div><h3>Conclusion</h3><p>Crocetin from tubular calyx of <em>Nyctanthes arbor-tristis</em> indicated to be potential wound healing phytoconstituent.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411021001255/pdfft?md5=4a975bfe0ee0b1e71f96e9f86795e85b&pid=1-s2.0-S2225411021001255-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72225337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrocotyle bonariensis Comm ex Lamm (Araliaceae) leaves extract inhibits IKs not IKr potassium currents: Potential implications for anti-arrhythmic therapy","authors":"Komla Kaboua ,&nbsp;Aklesso Mouzou ,&nbsp;Tcha Pakoussi ,&nbsp;Mindede Assih ,&nbsp;Aurelien Chatelier ,&nbsp;Aboudoulatifou Diallo ,&nbsp;Patrick Bois ,&nbsp;Jocelyn Bescond","doi":"10.1016/j.jtcme.2021.09.004","DOIUrl":"https://doi.org/10.1016/j.jtcme.2021.09.004","url":null,"abstract":"<div><h3>Background and aim</h3><p><em>Hydrocotyle bonariensis</em> Comm ex Lamm (Araliaceae) is one of these plants sufficiently exploited in traditional African medicine for its hypotensive effect. However, the pharmacological effects of those plants on cardiac functions are not well known. The potassium currents IKs and IKr, responsible for the repolarization of cardiac cell action potential, strongly influence the human cardiac rhythm. Therefore, modulators of these currents have a beneficial or undesirable medical importance in relation to cardiac arrhythmias. In order to optimize the therapeutic use of this medicinal plant, we studied the effects of hydro-ethanolic leaf extract of <em>Hydrocotyle bonariensi</em>s on both potassium currents.</p></div><div><h3>Experimental procedure</h3><p>The patch clamp experiments for IK currents recording were performed on the HEK 293 (Human Embryonic Kidney 293) cell line, stably transfected with either KCNQ1 and KCNE1 genes encoding the channel responsible for the \"IKs\" current (HEK293 IKs), or with hERG (human ether-a-go-go related gene) gene encoding \"IKr\" current (HEK293 IKr).</p></div><div><h3>Results and conclusion</h3><p>This study revealed that the hydro-ethanolic leaf extract of <em>H. bonariensis</em> significantly inhibits the slow potassium component (IKs) without altering the fast potassium component (IKr). The extract at 0.5 mg/ml decreases IKs conductance by 24 ± 4.1% (n = 6) without modifying its activation threshold suggesting a direct blockade of the slow potassium channel. This selective action of the extract on the IKs current reflects a class III anti-arrhythmic effect.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411021001206/pdfft?md5=9324a28143c4150d541cece51524fc9f&pid=1-s2.0-S2225411021001206-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72225675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detam 1 black soybean against cisplatin-induced acute ren failure on rat model via antioxidant, antiinflammatory and antiapoptosis potential","authors":"Wahyu Widowati ,&nbsp;Sijani Prahastuti ,&nbsp;Meilinah Hidayat ,&nbsp;Stella Tinia Hasianna ,&nbsp;Roro Wahyudianingsih ,&nbsp;The Fransiska Eltania ,&nbsp;Alya Mardhotillah Azizah ,&nbsp;Jenifer Kiem Aviani ,&nbsp;Mawar Subangkit ,&nbsp;Rr Anisa Siwianti Handayani ,&nbsp;Hanna Sari Widya Kusuma","doi":"10.1016/j.jtcme.2022.01.004","DOIUrl":"https://doi.org/10.1016/j.jtcme.2022.01.004","url":null,"abstract":"<div><h3>Background and aim</h3><p>Cis-Diamminedichloroplatinum (II) (Cisplatin) is one of the most synthetic anticancer drug but have several adverse effects and one of them is acute ren failure. Cisplatin can induce nephrotoxicity occur via the toxic generation of reactive oxygen species (ROS). Black soybean (<em>Glycine</em> max L. Merr.) has been reported contain high levels of phenolics and anthocyanins that has antioxidant activity. This study aims to determine the effect of ethanol extract of black soybean (EEBS) against cisplatin-induced nephrotoxicity in rats.</p></div><div><h3>Experimental procedure</h3><p>Cisplatin-induced nephrotoxicity rats treated with EEBS and the blood samples taken on days 0, 9, and 18. The effects of EEBS was evaluated by determining Interferon-γ (IFN-γ), Caspase-3 (Casp-3), and Interleukin-1β (IL-1β) expression using immunohistochemistry (IHC), blood urea nitrogen (BUN), Uric Acid (UA) content and catalase (CAT) content in the blood plasma with colorimetric assay kit.</p></div><div><h3>Results and conclusion</h3><p>Based on the results, EEBS treatment had successfully reduced pro-inflammatory cytokines IL-1β and IFN-γ, and improved physiological condition by lowering BUN and UA content while increasing CAT activity. No significant effect was found in Casp-3 expression. EEBS has potential to improve acute renal failure condition through inflammatory suppression and renal function improvement.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411022000049/pdfft?md5=595465f86c994f66ca0feaf558ed26a6&pid=1-s2.0-S2225411022000049-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72263825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Thai Mucuna pruriens (L.) DC. var. pruriens seeds on sexual behaviors and essential reproductive markers in chronic unpredictable mild stress mice","authors":"Pannawat Choowong-in ,&nbsp;Jintana Sattayasai ,&nbsp;Preecha Boonchoong ,&nbsp;Chanasorn Poodendaen ,&nbsp;Alexander TH. Wu ,&nbsp;Nareelak Tangsrisakda ,&nbsp;Tarinee Sawatpanich ,&nbsp;Supatcharee Arun ,&nbsp;Nongnut Uabundit ,&nbsp;Sitthichai Iamsaard","doi":"10.1016/j.jtcme.2021.12.001","DOIUrl":"https://doi.org/10.1016/j.jtcme.2021.12.001","url":null,"abstract":"<div><h3>Background and aims</h3><p>Chronic stress is a major common cause of male infertility. Many species of velvet beans are shown to be rich in <span>l</span>-DOPA. In Thai folklore medicine, seeds of <em>Mucuna pruriens</em> (L.) DC. var. pruriens (Thai Mhamui or T-MP) have been used for treating erectile dysfunction. This study aimed to determine <span>l</span>-DOPA levels in T-MP seed extract and investigate its preventive on sexual behaviors and reproductive parameter damages including essential proteins in chronic unpredictable mild stress (CUMS) mice.</p></div><div><h3>Experimental procedure</h3><p>Mice were divided into 4 groups: (I) control, (II) CUMS, (III) T-MP300 + CUMS, and (IV) T-MP600 + CUMS. Groups I and II received DW while groups III and IV were pretreated with the seed extracts (300 and 600 mg/kg BW) for 14 consecutive days before co-treatment with a randomly different CUMS/day (from 12 mild stressors) for 43 days.</p></div><div><h3>Results and conclusion</h3><p>T-MP seed extract contained <span>l</span>-DOPA approximately 10% of total dried weight. A dose of 600 mg/kg improved sexual performances and degenerative seminiferous epithelium in CUMS mice. Sperm qualities and testosterone level were elevated while corticosterone was decreased in co-treatment groups. T-MP-CUMS cotreated groups also improved expressions of AKAP4, AR, and TyrPho proteins in testis, epididymis, and sperm. T-MP increased StAR and CYP11A1 expressions in testis. It also suppressed testicular apoptosis via decreased expressions of Hsp70, caspases 3, and 9. T-MP seeds containing <span>l</span>-DOPA could improve sexual behaviors and essential reproductive proteins caused by CUMS.</p></div><div><h3>Section</h3><p>Natural Products;</p></div><div><h3>Taxonomy (classification by evise)</h3><p>Traditional Herbal Medicine; Animal Model; Histopathology.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411021001413/pdfft?md5=0113360f96c464aee2a9b78c200879c9&pid=1-s2.0-S2225411021001413-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72225673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of action involved in the anxiolytic-like effects of Hibalactone isolated from Hydrocotyle umbellata L.","authors":"Matheus Gabriel de Oliveira ,&nbsp;Lorrane Kelle da Silva Moreira ,&nbsp;Larissa Cordova Turones ,&nbsp;Dionys de Souza Almeida ,&nbsp;Aline Nazareth Martins ,&nbsp;Thiago Levi Silva Oliveira ,&nbsp;Vinicius Barreto da Silva ,&nbsp;Leonardo Luiz Borges ,&nbsp;Elson Alves Costa ,&nbsp;José Realino de Paula","doi":"10.1016/j.jtcme.2021.08.012","DOIUrl":"https://doi.org/10.1016/j.jtcme.2021.08.012","url":null,"abstract":"<div><h3>Background and aim</h3><p>Hibalactone (HB) is a lignan related to the anxiolytic-like effects of <em>Hydrocotyle umbellata</em> L. However, there is a need to understand better the mechanism of action of this lignan to support the ethnopharmacological uses of the species. This work aimed to evaluate by <em>in vivo</em> and <em>in silico</em> analysis the mechanism of action of HB involved in its anxiolytic-like effects.</p></div><div><h3>Experimental procedure</h3><p>The effects of HB in mice were evaluated on light-dark box (LDB) and elevated plus maze (EPM) tests. The participation of 5-HT_1A receptor and the benzodiazepine site of GABA_A receptor was evaluated to investigate the possible mechanism of action. <em>In silico</em> tools were used to better elucidate the anxiolytic-like effects of HB.</p></div><div><h3>Results</h3><p>Oral treatment with HB at a dose of 33 mg/kg showed an anxiolytic-like effect in the LDB and EPM tests. Besides that, the treatment altered the ethological parameters, frequency of head dips, and stretched-attend postures (SAP), important to better describe the anxiolytic profile of HB. Pretreatment with flumazenil (2 mg/kg) reverted the anxiolytic-like effect of HB on LDB and EPM tests. On the other hand, pretreatment with NAN-190 (0.5 mg/kg) not reverted the activity observed. <em>In silico</em> predictions revealed the potential of HB to increase GABAergic neurotransmission. Pharmacophore modelling and docking simulations showed that HB might interact with the α1β2γ2 GABA_A receptor.</p></div><div><h3>Conclusion</h3><p>Together, the results presented herein suggest that activation of the benzodiazepine site of the GABA_A receptor contributes to the anxiolytic-like effect of HB.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcme.2021.08.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72263858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM1 - Title Page","authors":"","doi":"10.1016/S2225-4110(22)00036-0","DOIUrl":"https://doi.org/10.1016/S2225-4110(22)00036-0","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411022000360/pdfft?md5=3e85a0d494e6b981349a40baaddc7a38&pid=1-s2.0-S2225411022000360-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72223459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM2- Aims & Scope","authors":"","doi":"10.1016/S2225-4110(22)00037-2","DOIUrl":"https://doi.org/10.1016/S2225-4110(22)00037-2","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411022000372/pdfft?md5=1b3f22df1cd5624bbd0639eecc1cf3a0&pid=1-s2.0-S2225411022000372-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72223460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Memory of Professor Kuo-Hsiung Lee (K. H. Lee)","authors":"Yi-Hong Tsai ,&nbsp;Fang-Rong Chang ,&nbsp;Yang Chang Wu","doi":"10.1016/j.jtcme.2022.02.003","DOIUrl":"https://doi.org/10.1016/j.jtcme.2022.02.003","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411022000190/pdfft?md5=ee96228ab011c83ed98f82a953f2993e&pid=1-s2.0-S2225411022000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72262161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM2- Aims & Scope","authors":"","doi":"10.1016/S2225-4110(22)00013-X","DOIUrl":"https://doi.org/10.1016/S2225-4110(22)00013-X","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S222541102200013X/pdfft?md5=6a971cd043974eaf758984cd5d59101c&pid=1-s2.0-S222541102200013X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72225953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FM1 - Title Page","authors":"","doi":"10.1016/S2225-4110(22)00012-8","DOIUrl":"https://doi.org/10.1016/S2225-4110(22)00012-8","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411022000128/pdfft?md5=3e660f6d0639bd1081cfd21c66c32de5&pid=1-s2.0-S2225411022000128-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72225954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}