Journal of Traditional and Complementary Medicine最新文献

筛选
英文 中文
Integrating single-cell and spatial transcriptomics to elucidate the crosstalk between cancer-associated fibroblasts and cancer cells in hepatocellular carcinoma with spleen-deficiency syndrome 整合单细胞和空间转录组学,阐明肝细胞癌伴脾脏缺失综合征中癌症相关成纤维细胞与癌细胞之间的串联关系
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-22 DOI: 10.1016/j.jtcme.2023.11.008
Qiuxia Chen , Jin Luo , Jiahui Liu , He Yu , Meiling Zhou , Ling Yu , Yan Chen , Shijun Zhang , Zhuomao Mo
{"title":"Integrating single-cell and spatial transcriptomics to elucidate the crosstalk between cancer-associated fibroblasts and cancer cells in hepatocellular carcinoma with spleen-deficiency syndrome","authors":"Qiuxia Chen ,&nbsp;Jin Luo ,&nbsp;Jiahui Liu ,&nbsp;He Yu ,&nbsp;Meiling Zhou ,&nbsp;Ling Yu ,&nbsp;Yan Chen ,&nbsp;Shijun Zhang ,&nbsp;Zhuomao Mo","doi":"10.1016/j.jtcme.2023.11.008","DOIUrl":"10.1016/j.jtcme.2023.11.008","url":null,"abstract":"<div><h3>Background and aim</h3><p>Most patients with hepatocellular carcinoma (HCC) in China have been diagnosed with spleen deficiency syndrome (SDS), which accelerates the progression of HCC by disrupting the tumor microenvironment homeostasis. This study aimed to investigate the intercellular crosstalk in HCC with SDS.</p></div><div><h3>Experimental procedure</h3><p>An HCC-SDS mouse model was established using orthotopic HCC transplantation based on reserpine-induced SDS. Single-cell data analysis and cancer cell prediction were conducted using Seurat and CopyKAT package, respectively. Intercellular interactions were explored using CellPhoneDB and CellChat and subsequently validated using co-culture assays, ELISA and histological staining. We performed pathway activity analysis using gene set variation analysis and the Seurat package. The extracellular matrix (ECM) remodeling was assessed using a gel contraction assay, atomic force microscopy, and Sirius red staining. The deconvolution of the spatial transcriptomics data using the “CARD” package based on single-cell data.</p></div><div><h3>Results and conclusion</h3><p>We successfully established the HCC-SDS mouse model. Twenty-nine clusters were identified. The interactions between cancer cells and cancer-associated fibroblasts (CAFs) were significantly enhanced via platelet-derived growth factor (PDGF) signaling in HCC-SDS. CAFs recruited in HCC-SDS lead to ECM remodeling and the activation of TGF-β signaling pathway. Deconvolution of the spatial transcriptome data revealed that CAFs physically surround cancer cells in HCC-SDS. This study reveals that the crosstalk of CAFs-cancer cells is crucial for the tumor-promoting effect of SDS. CAFs recruited by HCC via PDGFA may lead to ECM remodeling through activation of the TGF-β pathway, thereby forming a physical barrier to block immune cell infiltration under SDS.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 321-334"},"PeriodicalIF":4.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001220/pdfft?md5=2ef88edfa3b4522b4edf258637a5fb64&pid=1-s2.0-S2225411023001220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139292618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Qufeng tongluo decoction decreased proteinuria in diabetic mice by protecting podocytes via promoting autophagy 曲风通络汤通过促进自噬保护荚膜细胞,从而减少糖尿病小鼠的蛋白尿
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-21 DOI: 10.1016/j.jtcme.2023.11.007
Boran Ni , Yao Xiao , Ruojun Wei , Weijing Liu , Liwei Zhu , Yifan Liu , Zhichao Ruan , Jiamu Li , Shidong Wang , Jinxi Zhao , Weijun Huang
{"title":"Qufeng tongluo decoction decreased proteinuria in diabetic mice by protecting podocytes via promoting autophagy","authors":"Boran Ni ,&nbsp;Yao Xiao ,&nbsp;Ruojun Wei ,&nbsp;Weijing Liu ,&nbsp;Liwei Zhu ,&nbsp;Yifan Liu ,&nbsp;Zhichao Ruan ,&nbsp;Jiamu Li ,&nbsp;Shidong Wang ,&nbsp;Jinxi Zhao ,&nbsp;Weijun Huang","doi":"10.1016/j.jtcme.2023.11.007","DOIUrl":"10.1016/j.jtcme.2023.11.007","url":null,"abstract":"<div><h3>Background</h3><p>Diabetic kidney disease (DKD) is one of diabetic complications, which has become the leading cause of end-stage kidney disease. In addition to angiotensin-converting enzyme inhibitor/angiotensin II receptor blocker(ACEI/ARB) and sodium-glucose cotransporter-2 inhibitor (SGLT2i), traditional Chinese medicine (TCM) is an effective alternative treatment for DKD. In this study, the effect of Qufeng Tongluo (QFTL) decoction in decreasing proteinuria has been observed and its mechanism has been explored based on autophagy regulation in podocyte.</p></div><div><h3>Methods</h3><p>In vivo study, db/db mice were used as diabetes model and db/m mice as blank control. Db/db mice were treated with QFTL decoction, rapamycin, QFTL + 3-Methyladenine (3-MA), trehalose, chloroquine (CQ) and QFTL + CQ. Mice urinary albumin/creatinine (UACR), nephrin and autophagy related proteins (LC3 and p62) in kidney tissue were detected after intervention of 9 weeks. Transcriptomics was operated with the kidney tissue from model group and QFTL group. In vitro study, mouse podocyte clone-5 (MPC-5) cells were stimulated with hyperglycemic media (30 mmol/L glucose) or cultured with normal media. High-glucose-stimulated MPC-5 cells were treated with QFTL freeze-drying powder, rapamycin, CQ, trehalose, QFTL+3-MA and QFTL + CQ. Cytoskeletal actin, nephrin, ATG-5, ATG-7, Beclin-1, cathepsin L and cathepsin B were assessed. mRFP-GFP-LC3 was established by stubRFP-sensGFP-LC3 lentivirus transfection.</p></div><div><h3>Results</h3><p>QFTL decoction decreased the UACR and increased the nephrin level in kidney tissue and high-glucose-stimulated podocytes. Autophagy inhibitors, including 3-MA and chloroquine blocked the effects of QFTL decoction. Further study showed that QFTL decoction increased the LC3 expression and relieved p62 accumulation in podocytes of db/db mice. In high-glucose-stimulated MPC-5 cells, QFTL decoction rescued the inhibited LC3 and promoted the expression of ATG-5, ATG-7, and Beclin-1, while had no effect on the activity of cathepsin L and cathepsin B. Results of transcriptomics also showed that 51 autophagy related genes were regulated by QFTL decoction, including the genes of ATG10, SCOC, ATG4C, AMPK catalytic subunit, PI3K catalytic subunit, ATG3 and DRAM2.</p></div><div><h3>Conclusion</h3><p>QFTL decoction decreased proteinuria and protected podocytes in db/db mice by regulating autophagy.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 312-320"},"PeriodicalIF":4.5,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001219/pdfft?md5=d07f00c2512f8ebe69e9f4c1b19adf52&pid=1-s2.0-S2225411023001219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139299659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Peripheral pulse harmonic analysis and its clinical application: A systematic review 外周脉搏谐波分析及其临床应用:系统综述
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-20 DOI: 10.1016/j.jtcme.2023.11.006
Kun-Yuan Hsiao , Jiunn-Horng Kang , Yeong-Shing Wu , Hen-Hong Chang , Cheng-Ta Yang
{"title":"Peripheral pulse harmonic analysis and its clinical application: A systematic review","authors":"Kun-Yuan Hsiao ,&nbsp;Jiunn-Horng Kang ,&nbsp;Yeong-Shing Wu ,&nbsp;Hen-Hong Chang ,&nbsp;Cheng-Ta Yang","doi":"10.1016/j.jtcme.2023.11.006","DOIUrl":"10.1016/j.jtcme.2023.11.006","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;p&gt;Pulse harmonic analysis is a quantitative and objective methodology within traditional Chinese medicine (TCM) used to evaluate pulse characteristics. However, interpreting pulse wave data is challenging due to its inherent complexity. This study aims to provide a comprehensive review and comparison of existing human pulse wave harmonic analysis methods to elucidate their patterns and characteristics.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;A systematic review of clinical research reports published from 1990 to 2021 was conducted, focusing on variations in harmonic characteristics across different medical conditions and physiological states. Keyword searches included terms related to analysis methods (e.g., \"Pulse Spectrum,\" \"harmonic analysis,\" \"harmonic index\") and measured indicators (e.g., \"vascular response,\" \"PPG,\" \"Photoplethysmography,\" \"aortic,\" \"arterial,\" \"blood pressure\"). Supplementary research using PubMed's Mesh terms specifically targeted \"Pulse wave analysis\" within the methods and statistical analysis domain. Articles were filtered based on predefined criteria, including human participants and research related to pulse pressure or vascular volume changes. Conference papers, animal studies, and irrelevant research were excluded, with literature evaluation scales selected based on the retrieved research reports.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Initially, 6487 research reports were identified, and after screening, 50 reports were included in the review. The analysis revealed that low-frequency harmonics increase following vigorous activity or sympathetic excitation but decrease during rest or parasympathetic excitation. Cardiovascular patients exhibited elevated first harmonics associated with the liver meridian, while diabetes patients displayed weakened third harmonics related to the spleen meridian. Liver dysfunction was linked to changes in the first harmonic, and cancer patients showed signs of liver and kidney yin deficiency in the first and second harmonics. These findings underscore the potential of harmonic analysis for TCM disease diagnosis and organ assessment. Moreover, individuals with conditions such as liver dysfunction, cancer, and gynecological disorders displayed distinct intensity patterns across harmonics one through ten compared to healthy controls, albeit with some variations. Heterogeneity in these studies mainly stemmed from differences in measurement methods and study populations. Additionally, research suggested that factors like blood circulation and cognitive activity influenced harmonic intensity.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;p&gt;In summary, this report consolidates prior research on pulse wave harmonics analysis, revealing unique patterns associated with various physiological conditions. Despite limitations, such as limited sample sizes in previous studies, the observed associations between physiological states and harmonics hold promise for potential clinical applications. Th","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 223-236"},"PeriodicalIF":4.5,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001207/pdfft?md5=30babb8588217a198dc1d70c2df3ce04&pid=1-s2.0-S2225411023001207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139296191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Wound healing potential of Acacia catechu in streptozotocin-induced diabetic mice using in vivo and in silico approach” [J Tradit Compl Med 13 (5) (2023) 489–499] 采用体内和硅学方法研究刺槐对链脲佐菌素诱导的糖尿病小鼠伤口愈合的潜力☆"一文的更正
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-15 DOI: 10.1016/j.jtcme.2023.11.004
Vinayak P. Nakhate , Natasha S. Akojwar , Saurabh K. Sinha , Amarsinh D. Lomte , Mahaveer Dhobi , Prakash R. Itankar , Satyendra K. Prasad
{"title":"Corrigendum to “Wound healing potential of Acacia catechu in streptozotocin-induced diabetic mice using in vivo and in silico approach” [J Tradit Compl Med 13 (5) (2023) 489–499]","authors":"Vinayak P. Nakhate ,&nbsp;Natasha S. Akojwar ,&nbsp;Saurabh K. Sinha ,&nbsp;Amarsinh D. Lomte ,&nbsp;Mahaveer Dhobi ,&nbsp;Prakash R. Itankar ,&nbsp;Satyendra K. Prasad","doi":"10.1016/j.jtcme.2023.11.004","DOIUrl":"10.1016/j.jtcme.2023.11.004","url":null,"abstract":"","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Page 353"},"PeriodicalIF":4.5,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001189/pdfft?md5=4842447e1610f15ae37a81e3096c0e77&pid=1-s2.0-S2225411023001189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139303229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nootropic effect of Indian Royal Jelly against okadaic acid induced rat model of Alzheimer's disease: Inhibition of neuroinflammation and acetylcholineesterase 印度蜂王浆对 okadaic 酸诱导的阿尔茨海默病大鼠模型的促智作用:抑制神经炎症和乙酰胆碱酯酶
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-15 DOI: 10.1016/j.jtcme.2023.11.005
Rahul Dubey , L. Sathiyanarayanan , Sandeep Sankaran , S. Arulmozhi
{"title":"Nootropic effect of Indian Royal Jelly against okadaic acid induced rat model of Alzheimer's disease: Inhibition of neuroinflammation and acetylcholineesterase","authors":"Rahul Dubey ,&nbsp;L. Sathiyanarayanan ,&nbsp;Sandeep Sankaran ,&nbsp;S. Arulmozhi","doi":"10.1016/j.jtcme.2023.11.005","DOIUrl":"10.1016/j.jtcme.2023.11.005","url":null,"abstract":"<div><h3>Background</h3><p>Royal jelly is an anti-inflammatory, antioxidant, and neuroprotective bee product. There are several sources for royal jelly and one of them is Indian Royal Jelly (IRJ). However, the neuroprotective actions of IRJ and the underlying molecular mechanisms involved are not well known.</p></div><div><h3>Objective</h3><p>To evaluate the neuroprotective effect of IRJ in the okadaic acid (OKA)-induced Alzheimer's disease (AD) model in rats.</p></div><div><h3>Methods</h3><p>In male Wistar rats, OKA was intracerebroventricularly (ICV) administered, and from day 7, they were treated orally with IRJ or memantine for 21 days. Spatial and recognition learning and memory were evaluated from days 27–34; employing the Morris water maze (MWM) and the novel object recognition tests (NORT), respectively. <em>In vitro</em> biochemical measurements were taken of the cholinergic system and oxidative stress markers. In silico docking was used to find the role of tau protein kinase and phosphatase in the pharmacological action.</p></div><div><h3>Results</h3><p>In OKA-induced rats, IRJ decreased the escape latency and path length in MWM and increased the exploration time for novel objects and the discrimination index in NORT. ICV-OKA rats had higher free radicals and cytokines that caused inflammation and their level of free radical scavengers was back to normal with IRJ treatment. IRJ increased the level of acetylcholine and inhibited acetylcholinesterase. Moreover, the in silico docking study revealed the strong binding affinity of 10-hydroxy-2-decenoic acid (10-HDA), a bioactive constituent of IR, to the tau protein kinases and phosphatases.</p></div><div><h3>Conclusion</h3><p>IRJ may serve as a nootropic agent in the treatment of dementia, and owing to its capacity to prevent oxidative stress and neuroinflammation, and increase cholinergic tone; it has the potential to be explored as a novel strategy for the treatment of dementia and AD. More studies may be needed to develop 10-HDA as a novel drug entity for AD.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 300-311"},"PeriodicalIF":4.5,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001190/pdfft?md5=490b1a5e251ff3f8e98f5b97dc52125f&pid=1-s2.0-S2225411023001190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139294067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Betanin combined with virgin coconut oil inhibits neuroinflammation in aluminum chloride-induced toxicity in rats by regulating NLRP3 inflammasome 白桦脂素与初榨椰子油通过调节 NLRP3 炎症小体,抑制氯化铝诱导的大鼠毒性中的神经炎症
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-11 DOI: 10.1016/j.jtcme.2023.11.001
Baban S. Thawkar, Ginpreet Kaur
{"title":"Betanin combined with virgin coconut oil inhibits neuroinflammation in aluminum chloride-induced toxicity in rats by regulating NLRP3 inflammasome","authors":"Baban S. Thawkar,&nbsp;Ginpreet Kaur","doi":"10.1016/j.jtcme.2023.11.001","DOIUrl":"10.1016/j.jtcme.2023.11.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>Activating NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) is crucial in the pathogenesis of Alzheimer's disease (AD). A multimodal treatment intervention is the most feasible way to alter the course of AD progression. Hence, the current study was conducted to study the combination of betanin (BET) and virgin coconut oil (VCO) on NLRP3 regulation in aluminum chloride-induced AD in Wistar rats.</p></div><div><h3>Experimental procedure</h3><p>BET (100,200 mg/kg) and VCO (1, 5 g/kg) alone and in combination (BET 100 mg/kg + VCO 1 g/kg and BET 200 mg/kg + VCO 5 g/kg) were given orally for 42 days. On day 21 and 42nd, the behavioral test was performed to check the animal's cognition. Acetylcholinesterase (AChE) activity, oxidative stress markers, estimation of NLRP3 and IL-1β, and histological examinations were conducted in the hippocampus (H) and cortex (C).</p></div><div><h3>Results and conclusion</h3><p>Treatment with BET and VCO alone or combined improved behavioral characteristics (MWM and PA p &lt; 0.0001; EPM p = 0.5184), inhibited AChE activity (C, p = 0.0101; H, p &lt; 0.0001), and lowered oxidative stress in the brain. Also, combination treatment restored the levels of NLRP3 (C, p = 0.0062; H, p &lt; 0.0001) and IL1β (C, p = 0.0005; H, p = 0.0098). The combination treatment significantly reduced the degree of neuronal degeneration, amyloid deposition, and necrosis in the brain tissue. The current study revealed that the combination strategy effectively controlled neuroinflammation via modulation of the NLRP3 inflammasome pathway, paving the way for the new treatment.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 3","pages":"Pages 287-299"},"PeriodicalIF":4.5,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001153/pdfft?md5=4382b25dee87f482f508aac225787d02&pid=1-s2.0-S2225411023001153-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135669817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Eriodictyol attenuates doxorubicin-induced nephropathy by activating the AMPK/Nrf2 signalling pathway 桉叶油醇通过激活 AMPK/Nrf2 信号通路减轻多柔比星诱发的肾病
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-08 DOI: 10.1016/j.jtcme.2023.11.003
Rehab Mustafa Badi , Eman Farok Khaleel , Huda Hammed Satti , Rehan Monir
{"title":"Eriodictyol attenuates doxorubicin-induced nephropathy by activating the AMPK/Nrf2 signalling pathway","authors":"Rehab Mustafa Badi ,&nbsp;Eman Farok Khaleel ,&nbsp;Huda Hammed Satti ,&nbsp;Rehan Monir","doi":"10.1016/j.jtcme.2023.11.003","DOIUrl":"10.1016/j.jtcme.2023.11.003","url":null,"abstract":"<div><p>Doxorubicin (DOX), an anthracycline chemotherapy, plays a prominent role in the treatment of various cancers. Unfortunately, its nephrotoxic effects limit its dosing and expose cancer survivors to increased morbidity and mortality. This study examined the nephroprotective effects of eriodictyol, a natural polyphenolic flavanone, in DOX-treated rats and the molecular pathways involved. Forty adult rats were divided into five groups (8/group): Control; eriodictyol (20 mg/kg/day); DOX (2.5 mg/kg, twice/week); DOX + Eriodictyol; and DOX + Eriodictyol + Compound C (CC), an AMPK inhibitor (0.2 mg/kg/day). Experiments continued for 21 days. Eriodictyol administration in DOX-treated rats reduced their fasting glucose levels and increased food intake, final body weight, and kidney weight, improved kidney function, prevented glomerular and tubular damage, and reduced collagen deposition and renal TGF-β1 mRNA levels. Furthermore, eriodictyol reduced their renal levels of Bax, caspase-3, and cytochrome-c; and enhanced the levels of Bcl2. Noticeably, in the kidneys of both controls and DOX-treated rats, eriodictyol increased levels of phosphorylated-AMPK(Thr<sup>172</sup>) but not AMPK mRNA nor protein levels. Also, in the same two groups, eriodictyol increased mRNA and nuclear Nrf2 levels, and levels of glutathione, superoxide dismutase, catalase, and hemeoxygenase-1, but reduced the levels of malonaldehyde, TNF-α, and mRNA, total, and nuclear levels of NF-κB. All the detected nephroprotective effects and improvements in the levels of markers of oxidation and inflammation were prevented by coadministration of CC. In conclusion, the coadministration of eriodictyol and DOX alleviates DOX-induced renal damage. In renal tissues, eriodictyol is an AMPK activator and its nephroprotective antioxidant and anti-inflammatory effects are AMPK-dependent.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 203-214"},"PeriodicalIF":4.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001177/pdfft?md5=47e15311f5f67e2a26523a576a0cb419&pid=1-s2.0-S2225411023001177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135515715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effectiveness of Chinese herbal medicine compared with angiotensin II receptor blockers in patients with diabetic kidney disease: A hospital-based matched cohort study 中药与血管紧张素 II 受体阻滞剂对糖尿病肾病患者的疗效比较:一项基于医院的匹配队列研究
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-08 DOI: 10.1016/j.jtcme.2023.11.002
Yuan-Ching Liao , Mei-Yao Wu , Yu-Chuen Huang , Che-Yi Chou , Hung-Rong Yen
{"title":"Effectiveness of Chinese herbal medicine compared with angiotensin II receptor blockers in patients with diabetic kidney disease: A hospital-based matched cohort study","authors":"Yuan-Ching Liao ,&nbsp;Mei-Yao Wu ,&nbsp;Yu-Chuen Huang ,&nbsp;Che-Yi Chou ,&nbsp;Hung-Rong Yen","doi":"10.1016/j.jtcme.2023.11.002","DOIUrl":"10.1016/j.jtcme.2023.11.002","url":null,"abstract":"<div><p>Angiotensin II receptor blockers (ARBs) are one of the standard treatments for diabetic kidney disease (DKD). Some patients may opt for Chinese herbal medicine (CHM) of their own free will. However, there is no real-world evidence regarding the effectiveness and safety of CHM. We aimed to explore the effectiveness of CHM for DKD in comparison to ARBs. We enrolled 732 DKD patients (72 used only CHM and 661 used ARBs) from 2007 to 2016, and all patients were followed until December 2016 at China Medical University Hospital in Taiwan. A total of 355 ARB users and 71 CHM users were analyzed after propensity score matching. The estimated glomerular filtration rate (eGFR) after treatment was 84.9 ± 28.1 ml/min/1.73 m<sup>2</sup> in CHM users, which was higher than that (67.8 ± 35.4 ml/min/1.73 m<sup>2</sup>) in ARB users (p &lt; 0.001). The change in the eGFR was −6.0 ± 21.4 ml/min/1.73 m<sup>2</sup> in CHM users and −12.9 ± 24.8 ml/min/1.73 m<sup>2</sup> in ARB users (p = 0.029). The blood urea nitrogen (BUN) and creatinine levels of patients taking CHM were 22 ± 16 mg/dl and 0.9 ± 0.4 mg/dl, respectively, and were lower than those (30 ± 28 mg/dl and 1.7 ± 2.0 mg/dl) of patients taking ARBs (p = 0.025 and p = 0.003). Using linear regression with adjustments for age, sex, BMI, baseline eGFR, and HbA1c levels, we found that the declines in the eGFR/baseline eGFR and changes in the urine albumin–creatinine ratio (ACR) were comparable between the two groups (p = 0.86 and 0.73). This study suggests that CHM may have comparable effectiveness to ARBs, which provides insights for further investigations.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 215-222"},"PeriodicalIF":4.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001165/pdfft?md5=16d6e923a0eba64f3f96abd00e92d2df&pid=1-s2.0-S2225411023001165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135515825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Network pharmacology analysis of a patented Chinese herbal medicine for alleviating anxiety disorder in in vitro fertilization-embryo transfer 体外受精-胚胎移植中缓解焦虑症的专利中药的网络药理学分析
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-02 DOI: 10.1016/j.jtcme.2023.10.003
Chang Liu , Weihuan Hu , Xiaoling Feng , Fan Qu
{"title":"Network pharmacology analysis of a patented Chinese herbal medicine for alleviating anxiety disorder in in vitro fertilization-embryo transfer","authors":"Chang Liu ,&nbsp;Weihuan Hu ,&nbsp;Xiaoling Feng ,&nbsp;Fan Qu","doi":"10.1016/j.jtcme.2023.10.003","DOIUrl":"10.1016/j.jtcme.2023.10.003","url":null,"abstract":"<div><h3>Objective</h3><p>Qu's formula 3 (QUF3) is a patented Chinese herbal medicine used to alleviate anxiety disorders during in vitro fertilization-embryo transfer (IVF-ET). This study aimed to identify the potential active constituents and molecular mechanisms of action of QUF3 in alleviating anxiety disorders during IVF-ET.</p></div><div><h3>Methods</h3><p>The active constituents of QUF3 were identified from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and literatures. Potential targets of anxiety disorder and IVF-ET were identified using GeneCards, Online Mendelian Inheritance in Man, and the UniProt Database. Protein-protein interaction (PPI) network, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to identify the potential mechanisms. Molecular docking and molecular dynamics (MD) simulations were performed to visualize and verify the results.</p></div><div><h3>Results</h3><p>Quercetin, sophoranol, luteolin, kaempferol, and neurotoxin inhibitors were identified as the TOP 5 active constituents of QUF3. Forty common targets were shared among QUF3, anxiety disorders, and IVF-ET. Tumour necrosis factor, interleukin-6, vascular endothelial growth factor A, epidermal growth factor, interleukin-1B, cellular tumour antigen p53, matrix metalloproteinase-9, and oestrogen receptor were identified as the TOP 8 potential targets through PPI analysis. A total of 697 biological processes, 20 cellular components, and 54 molecular functions were identified. Further, 91 KEGG pathways were revealed to be enriched. The TOP 5 active constituents were verified to have good binding activity with the TOP 8 potential targets using molecular docking and MD simulations.</p></div><div><h3>Conclusions</h3><p>The mechanism of QUF3 in alleviating anxiety disorders in patients undergoing IVF-ET may be related to the interleukin-17 and tumour necrosis factor signalling pathways, inhibiting inflammatory responses and antioxidants, which may provide a solid foundation for the clinical application and further study of QUF3.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"14 2","pages":"Pages 191-202"},"PeriodicalIF":4.5,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2225411023001104/pdfft?md5=2f9ff6fd6e86772582da2fa8befc3590&pid=1-s2.0-S2225411023001104-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135372245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prosopis juliflora (Sw.) DC.induces apoptotic-like programmed cell death in Leishmania donovani via over production of oxidative stress, mitochondrial dysfunction and ATP depletion 黄花拟豆(Sw.)直流。通过过度产生氧化应激、线粒体功能障碍和ATP耗竭,诱导多诺瓦利什曼原虫的凋亡样程序性细胞死亡
IF 4.5 3区 医学
Journal of Traditional and Complementary Medicine Pub Date : 2023-11-01 DOI: 10.1016/j.jtcme.2023.06.003
Bhimashankar Utage , Milind Patole , Punam Nagvenkar , Rajesh Gacche
{"title":"Prosopis juliflora (Sw.) DC.induces apoptotic-like programmed cell death in Leishmania donovani via over production of oxidative stress, mitochondrial dysfunction and ATP depletion","authors":"Bhimashankar Utage ,&nbsp;Milind Patole ,&nbsp;Punam Nagvenkar ,&nbsp;Rajesh Gacche","doi":"10.1016/j.jtcme.2023.06.003","DOIUrl":"10.1016/j.jtcme.2023.06.003","url":null,"abstract":"<div><h3>Background</h3><p>Leishmaniasis is endemic in more than 60 countries with a large number of mortality cases. The current chemotherapy approaches employed for managing the leishmaniasis is associated with severe side effects. Therefore there is a need to develop effective, safe, and cost affordable antileishmanial drug candidates.</p></div><div><h3>Purpose of the study</h3><p>This study was designed to evaluate the <em>in vitro</em> antileishmanial activity of a <em>Prosopis juliflora</em> leaves extract (PJLME) towards the <em>Leishmania donovani</em> parasites.</p></div><div><h3>Material and methods</h3><p>PJLME was evaluated for its cytotoxicity against the <em>L. donovani</em> parasites and the mouse macrophage cells. Further, various <em>in vitro</em> experiments like ROS assay, mitochondrial membrane potential assay, annexin v assay, cell cycle assay, and caspase 3/7 assay were performed to understand the mechanism of cell death. Phytochemical profiling of <em>P. juliflora</em>was performed by utilizing HPTLC and GC-MS analysis.</p></div><div><h3>Results</h3><p>PJLME demonstrated antileishmanial activity at a remarkably lower concentration of IC<sub>50</sub> 6.5 μg/mL. Of note, interestingly PJLME IC<sub>50</sub> concentration has not demonstrated cytotoxicity against the mouse macrophage cell line. Performed experiments confirmed ROS inducing potential of PJLME which adversely affected the mitochondrial membrane potential and caused loss of mitochondrial membrane potential and thereby ATP levels. PJLME also arrested the cell cycle and induced apoptotic-like cell death in PJLME treated <em>L. donovani</em> promastigotes.</p></div><div><h3>Conclusion</h3><p>The results clearly established the significance of <em>Prosopis juliflora</em> as an effective and safe natural resource for managing visceral leishmaniasis. The findings can be used as a baseline reference for developing novel leads/formulations for effective management of visceral leishmaniasis.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"13 6","pages":"Pages 611-622"},"PeriodicalIF":4.5,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41537489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信