止龙活血通瘀胶囊通过改善内皮细胞功能障碍减轻心肌纤维化

IF 3.3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Tao Bi , Yanan Zhou , Linshen Mao , Pan Liang , Jiali Liu , Luyin Yang , Guilin Ren , Maryam Mazhar , Hongping Shen , Ping Liu , Roman Spáčil , Qing Guo , Gang Luo , Sijin Yang , Wei Ren
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引用次数: 0

摘要

背景和目的芝龙化痰通瘀胶囊是一种疗效确切的经典中药。内皮细胞(EC)功能障碍在心肌纤维化(MF)过程中起着重要作用。但ZL胶囊对心肌纤维化过程中内皮细胞功能障碍的治疗效果尚不清楚。实验过程在体内注射异丙肾上腺素14天,建立心肌纤维化模型,同时观察ZL胶囊对心肌纤维化的治疗效果。我们采用生物标志物检查、超声心动图和心肌纤维化情况的综合方法,使用苏木精-伊红染色、Masson 染色和天狼星红染色来评估 ZL 胶囊对 MF 的疗效。随后,进行了全面的免疫荧光染色,以评估 ZL 胶囊对心肌细胞功能障碍的治疗效果。结果与结论在实验之前,对已发表的单细胞测序数据进行了分析,发现心肌细胞功能障碍发挥了重要作用。进一步的药理研究结果表明,ZL 胶囊能减轻纤维化损伤和胶原纤维沉积。机理研究结果表明,内皮细胞向间质转化(EndMT)和MHC-II(MHC-II)在EC中的表达得到了改善。此外,ZL胶囊还能通过干预EC介导的CD4+T细胞活化,减轻中风期间的炎症反应。我们首次提供了证据,证明ZL胶囊可通过调节内皮细胞间质转化和MHC-II的表达,缓解心肌细胞功能障碍,从而改善心肌纤维化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction

Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction

Background and aim

Zhilong Huoxue Tongyu (ZL) capsule is a classical traditional Chinese medicine (TCM) with satisfactory curative effects. Endothelial cell (EC) dysfunction plays an important role during myocardial fibrosis (MF). But the therapeutic effect of ZL capsule on EC dysfunction remains unknown in the development of MF. This study aims to investigate the effect of ZL capsule on EC dysfunction during MF in vivo.

Experimental procedure

The model of MF is established in vivo by injecting isoproterenol for 14 days, simultaneously, we examined the therapeutic effect of ZL capsule on MF in vivo. An integrative approach combining biomarker examination, echocardiography and myocardial fibrosis condition using Hematoxylin-eosin staining, Masson staining, and Sirius red staining were performed to assess the efficacy of ZL capsule against MF. Subsequently, comprehensive immunofluorescence staining was performed to evaluate the therapeutic effect of ZL capsule on EC dysfunction.

Results and conclusion

Prior to experiments, analysis of the published single-cell sequencing data was performed and it was discovered that EC dysfunction plays an important role. Further pharmacological results showed that ZL capsule could alleviate fibrosis injury and collagen fiber deposition. The mechanism investigation results showed that the endothelial-to-mesenchymal transition (EndMT) and MHC class-II (MHC-II) expression in EC were improved. In addition, ZL capsule can attenuate the inflammatory response during MF by intervening the activation of CD4+T cell mediated by EC. For the first time, we provided evidence that ZL capsule could improve MF by alleviating EC dysfunction via the regulation of EndMT and expression of MHC-II.

Taxonomy (classification by evise)

Myocardial fibrosis, Chinese Herbal Medicine, Traditional Medicine, Endothelium, dysfunction, Endothelial-to-mesenchymal transition.

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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
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